Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
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| antimicrobial susceptibilities of multidrug-resistant campylobacter jejuni and c. coli strains: in vitro activities of 20 antimicrobial agents. | there is a paucity of information regarding antimicrobial agents that are suitable to treat severe infections caused by multidrug-resistant campylobacter spp. our aim was to identify agents that are potentially effective against multiresistant campylobacter strains. the in vitro activities of 20 antimicrobial agents against 238 campylobacter strains were analyzed by determining mics by the agar plate dilution method or the etest. these strains were selected from 1,808 campylobacter isolates coll ... | 2010 | 20038624 |
| lyophilization prior to direct dna extraction from bovine feces improves the quantification of escherichia coli o157:h7 and campylobacter jejuni. | lyophilization was used to concentrate bovine feces prior to dna extraction and analysis using real-time pcr. lyophilization significantly improved the sensitivity of detection compared to that in fresh feces and was associated with reliable quantification of both escherichia coli o157:h7 and campylobacter jejuni bacteria present in feces at concentrations ranging between 2 log(10) and 6 log(10) cfu g(-)(1). | 2010 | 20038690 |
| attachment, invasion, and translocation of campylobacter jejuni in pig small-intestinal epithelial cells. | campylobacters are susceptible to environmental conditions such as starvation, temperature, and oxidative stress. species such as campylobacter jejuni have developed a number of mechanisms for responding to these conditions. we conducted a study to investigate whether survival of c. jejuni and pathogen-host cell interactions such as adherence, invasiveness, and intraepithelial survival in pig small-intestinal (psi) epithelial cells are altered in response to starvation, changes in temperature, a ... | 2010 | 20039793 |
| spatio-temporal epidemiology of campylobacter jejuni enteritis, in an area of northwest england, 2000-2002. | a total of 969 isolates of campylobacter jejuni originating in the preston, lancashire postcode district over a 3-year period were characterized using multi-locus sequence typing. recently developed statistical methods and a genetic model were used to investigate temporal, spatial, spatio-temporal and genetic variation in human c. jejuni infections. the analysis of the data showed statistically significant seasonal variation, spatial clustering, small-scale spatio-temporal clustering and spatio- ... | 2010 | 20202286 |
| reduction of campylobacter jejuni and campylobacter coli in poultry skin by fruit extracts. | campylobacter spp. are a major cause of foodborne bacterial gastroenteritis in humans, and current methods to control campylobacter contamination in foods are not completely successful. plants are a promising source of antimicrobial agents, particularly given the growing interest in "all natural" foods. in this study, the antimicrobial activity of extracts from 28 edible plants against campylobacter jejuni and campylobacter coli was evaluated in vitro and in a poultry skin model. nine of 28 extr ... | 2010 | 20202332 |
| where does campylobacter come from? a molecular odyssey. | 2010 | 20204754 | |
| survey of campylobacter jejuni in retail chicken meat products by application of a quantitative pcr protocol. | campylobacter-contaminated food products are currently the cause of the highest number of gastroenteritis cases in developed countries. apart for biosafety measures at the primary production level, no other official control measures are currently in place for its control. this is partly due to the lack of quantitative data regarding the prevalence and contamination level of different food products by campylobacter spp. that does not allow for quantitative risk assessment. pcr-based methods, appl ... | 2010 | 20207040 |
| crystal structure of activated chey1 from helicobacter pylori. | chemotaxis is an important virulence factor for helicobacter pylori colonization and infection. the chemotactic system of h. pylori is marked by the presence of multiple response regulators: chey1, one chey-like-containing chea protein (cheay2), and three chev proteins. recent studies have demonstrated that these molecules play unique roles in the chemotactic signal transduction mechanisms of h. pylori. here we report the crystal structures of bef(3(-)-activated chey1 from h. pylori resolved to ... | 2010 | 20207758 |
| influence of refuse sites on the prevalence of campylobacter spp. and salmonella serovars in seagulls. | wild animals are well-known reservoirs of campylobacter and salmonella. we investigated the influence of insalubrious diets on the prevalence of both enterobacteria in seagulls. campylobacter occurrence in gull chicks sampled along the northeastern iberian coast was directly related to the degree of refuse consumption. high salmonella values from the sampling sites did not reflect any dietary relationship. | 2010 | 20208027 |
| human intestinal muc17 mucin augments intestinal cell restitution and enhances healing of experimental colitis. | the membrane-bound mucins, muc17 (human) and muc3 (mouse), are highly expressed on the apical surface of intestinal epithelia and are thought to be cytoprotective. the extracellular regions of these mucins contain egf-like cys-rich segments (crd1 and crd2) connected by an intervening linker domain (l). the purpose of this study was to determine the functional activity of human muc17 membrane-bound mucin. methods: endogenous muc17 was inhibited in ls174t colon cells by stable transfection of a sm ... | 2010 | 20211273 |
| requirement of nmb0065 for connecting assembly and export of sialic acid capsular polysaccharides in neisseria meningitidis. | capsule expression in neisseria meningitidis is encoded by the cps locus comprised of genes required for biosynthesis and surface translocation. located adjacent to the gene encoding the polysialyltransferase in serogroups expressing sialic acid-containing capsule, nmb0065 is likely a member of the cps locus, but it is not found in serogroups a or x that express non-sialic acid capsules. to further understand its role in cps expression, nmb0065 mutants were created in the serogroups b, c and y s ... | 2010 | 20215001 |
| descriptive proteomic analysis shows protein variability between closely related clinical isolates of mycobacterium tuberculosis. | the use of isobaric tags such as itraq allows the relative and absolute quantification of hundreds of proteins in a single experiment for up to eight different samples. more classical techniques such as 2-de can offer a complimentary approach for the analysis of complex protein samples. in this study, the proteomes of secreted and cytosolic proteins of genetically closely related strains of mycobacterium tuberculosis were analyzed. analysis of 2-d gels afforded 28 spots with variations in protei ... | 2010 | 20217870 |
| comparative genomics and proteomics of helicobacter mustelae, an ulcerogenic and carcinogenic gastric pathogen. | helicobacter mustelae causes gastritis, ulcers and gastric cancer in ferrets and other mustelids. h. mustelae remains the only helicobacter other than h. pylori that causes gastric ulceration and cancer in its natural host. to improve understanding of h. mustelae pathogenesis, and the ulcerogenic and carcinogenic potential of helicobacters in general, we sequenced the h. mustelae genome, and identified 425 expressed proteins in the envelope and cytosolic proteome. | 2010 | 20219135 |
| helicobacter pullorum outbreak in c57bl/6ntac and c3h/hentac barrier-maintained mice. | helicobacter pullorum is a bacterial pathogen in humans. by using microaerobic culture techniques, h. pullorum was isolated from the feces of barrier-maintained mice and identified, on the basis of biochemical, restriction fragment length polymorphism, and 16s rrna gene sequence analyses. this finding presents an opportunity to study h. pullorum pathogenesis in mice. | 2010 | 20220161 |
| performance of matrix-assisted laser desorption ionization-time of flight mass spectrometry for identification of bacterial strains routinely isolated in a clinical microbiology laboratory. | matrix-assisted laser desorption ionization-time of flight mass spectrometry (maldi-tof ms) has recently been introduced in diagnostic microbiology laboratories for the identification of bacterial and yeast strains isolated from clinical samples. in the present study, we prospectively compared maldi-tof ms to the conventional phenotypic method for the identification of routine isolates. colonies were analyzed by maldi-tof ms either by direct deposition on the target plate or after a formic acid- ... | 2010 | 20220166 |
| risk factors for indigenous campylobacter jejuni and campylobacter coli infections in the netherlands: a case-control study. | a case-control study comprising 1315 campylobacter jejuni cases, 121 campylobacter coli cases and 3409 frequency-matched controls was conducted in the netherlands in 2002-2003. risk factors for both c. jejuni and c. coli enteritis were consumption of undercooked meat and barbecued meat, ownership of cats and use of proton pump inhibitors. consumption of chicken was a predominant risk factor for c. jejuni enteritis, but many additional risk factors were identified. unique risk factors for c. coli ... | 2010 | 20223048 |
| phenotypic and genotypic antimicrobial resistance profiles of campylobacter jejuni isolated from cattle, sheep, and free-range poultry faeces. | minimum inhibitory concentrations (mic) of 13 antimicrobial agents were determined by broth microdilution for 72 campylobacter jejuni strains from livestock. twenty-three (31.9%) isolates were fully susceptible; all isolates were susceptible to erythromycin, chloramphenicol, streptomycin, gentamicin, sulfamethoxazole, and meropenem, and all but one to kanamycin. resistance to quinolones was highest (52.8%), reaching similar values among poultry, dairy cattle, and sheep, but lower in beef cattle. ... | 2010 | 20224816 |
| in vitro susceptibility to antimicrobial agents and ultrastructural characteristics related to swimming motility and drug action in campylobacter jejuni and c. coli. | campylobacter jejuni has recently been noted as the most common cause of bacterial food-borne diseases in japan. in this study, we examined in vitro susceptibility to 36 antimicrobial agents of 109 strains of c. jejuni and c. coli isolated from chickens and patients with enteritis or guillain-barré syndrome from 1996 to 2009. among these agents, carbapenems (imipenem, meropenem, panipenem, and biapenem) showed the greatest activity [minimal inhibitory concentration (mic)(90), 0.03-0.125 microg/m ... | 2010 | 20225076 |
| mutant prevention concentrations of fluoroquinolones against campylobacter jejuni isolated from chicken. | the mutant prevention concentration (mpc) and mutant selection window (msw) concepts have been used to evaluate antibiotic concentration ranges that prevent the emergence of antibiotic resistant mutants. campylobacter jejuni is highly mutable to fluoroquinolone (fq) antibiotics, but it is unknown if the mpc concept can be used to prevent mutant emergence. in this study, the mpcs of three fqs including enrofloxacin, norfloxacin and ciprofloxacin were determined using 13 c. jejuni isolates. also, ... | 2010 | 20226601 |
| a localized multimeric anchor attaches the caulobacter holdfast to the cell pole. | caulobacter crescentus attachment is mediated by the holdfast, a complex of polysaccharide anchored to the cell by hfaa, hfab and hfad. we show that all three proteins are surface exposed outer membrane (om) proteins. hfaa is similar to fimbrial proteins and assembles into a high molecular weight (hmw) form requiring hfad, but not holdfast polysaccharide. the hfad hmw form is dependent on hfaa but not on holdfast polysaccharide. we show that hfaa and hfad form homomultimers and that they require ... | 2010 | 20233308 |
| proteins involved in campylobacter jejuni 81-176 recovery after high-pressure treatment. | campylobacteriosis has been recognized as the major bacterial food-borne infection worldwide. campylobacter, especially c. jejuni, contaminate mainly poultry meat. although more sensitive than other food-borne pathogens to many stresses, c. jejuni can survive food processing and go on to reach its final reservoir (the human gut). genomic analyses of this organism indicate a lack of genes described in other gram-negative bacteria to overcome stresses. the high-pressure recovery response of c. jej ... | 2010 | 20233379 |
| the chemical-in-plug bacterial chemotaxis assay is prone to false positive responses. | abstract: | 2010 | 20233446 |
| crystal structure and functional insight of hp0420-homolog from helicobacter felis. | helicobacter pylori infect more than half of the world's population and are considered a cause of peptic ulcer disease and gastric cancer. recently, hypothetical gene hp0421 was identified in h. pylori as a cholesterol alpha-glucosyltransferase, which is required to synthesize cholesteryl glucosides, essential cell wall components of the bacteria. in the same gene-cluster, hp0420 was co-identified, whose function remains unknown. here we report the crystal structure of hp0420-homolog of h. felis ... | 2010 | 20302842 |
| differential regulation of the multiple flagellins in spirochetes. | the expression of flagellin genes in most bacteria is typically regulated by the flagellum-specific sigma(28) factor flia, and an anti-sigma(28) factor, flgm. however, the regulatory hierarchy in several bacteria that have multiple flagellins is more complex. in these bacteria, the flagellin genes are often transcribed by at least two different sigma factors. the flagellar filament in spirochetes consists of one to three flab core proteins and at least one flaa sheath protein. here, the genetica ... | 2010 | 20304988 |
| functional characterization of the ilpa protein of vibrio vulnificus as an adhesin and its role in bacterial pathogenesis. | vibrio vulnificus is a gram-negative bacterium that causes a fatal septicemia. one of its virulence factors is a membrane-bound lipoprotein, ilpa, which can induce cytokine production in human immune cells. in the present study, the role of ilpa as an adhesion molecule was investigated. an ilpa-deleted v. vulnificus mutant showed significantly decreased adherence to int-407 human intestinal epithelial cells, which in turn resulted in reduced cytotoxicity. the deltailpa mutant recovered the adher ... | 2010 | 20308294 |
| probiotic colonization of the adherent mucus layer of ht29mtxe12 cells attenuates campylobacter jejuni virulence properties. | the ht29mtxe12 (e12) cell line harbors an adherent mucus layer, providing a novel technique to model mucosal infection in vitro. in this study, we have characterized the interaction of campylobacter jejuni with the e12 cell line and exploited its unique mucus layer to examine the potential efficacy of probiotic treatment to attenuate c. jejuni virulence properties. c. jejuni 81-176 colonized and reproduced in e12 mucus. adhesion to and internalization of c. jejuni were enhanced in e12 cells harb ... | 2010 | 20308300 |
| infection of human mucosal tissue by pseudomonas aeruginosa requires sequential and mutually dependent virulence factors and a novel pilus-associated adhesin. | tissue damage predisposes humans to life-threatening disseminating infection by the opportunistic pathogen pseudomonas aeruginosa. bacterial adherence to host tissue is a critical first step in this infection process. it is well established that p. aeruginosa attachment to host cells involves type iv pili (tfp), which are retractile surface fibres. the molecular details of attachment and the identity of the bacterial adhesin and host receptor remain controversial. using a mucosal epithelium mode ... | 2010 | 20331639 |
| overexpression and topology of bacterial oligosaccharyltransferase pglb. | campylobacter jejuni contains a post-translational n-glycosylation system in which a stt3 homologue, pglb, functions as the oligosaccharyltransferase. herein, we established a method for obtaining relatively large quantities of homogenous pglb proteins. pglb was overexpressed in escherichia coli c43(de3) at a level of 1 mg/l cell cultures. the activity of purified pglb was verified using a chemically synthesized sugar donor: n-acetylgalactosamine-diphospho-undecaprenyl (galnac-pp-und) and a synt ... | 2010 | 20331969 |
| enhanced antigen uptake by dendritic cells induced by the b pentamer of the type ii heat-labile enterotoxin lt-iia requires engagement of tlr2. | the potent mucosal adjuvant properties of the type ii heat-labile enterotoxin lt-iia of escherichia coli are dependent upon binding of the b pentamer of the enterotoxin (lt-iia-b(5)) to ganglioside receptors on immunocompetent cells. to evaluate the immunomodulatory activities of lt-iia-b(5), in vitro experiments employing bone marrow-derived dendritic cells (bmdc) were performed. uptake of ova-fitc, a model antigen (ag), was enhanced by treatment of bmdc with lt-iia-b5, but not by treatment of ... | 2010 | 20332049 |
| helicobacter pylori lipopolysaccharide is synthesized via a novel pathway with an evolutionary connection to protein n-glycosylation. | lipopolysaccharide (lps) is a major component on the surface of gram negative bacteria and is composed of lipid a-core and the o antigen polysaccharide. o polysaccharides of the gastric pathogen helicobacter pylori contain lewis antigens, mimicking glycan structures produced by human cells. the interaction of lewis antigens with human dendritic cells induces a modulation of the immune response, contributing to the h. pylori virulence. the amount and position of lewis antigens in the lps varies a ... | 2010 | 20333251 |
| is there a risk of cancer development after campylobacter infection? | all campylobacter jejuni species produce a genotoxin, which induce dna double strand breaks, could lead to an increased risk of cancer especially in the gastro-intestinal tract. | 2010 | 20334473 |
| multi-locus sequence types of campylobacter carried by flies and slugs acquired from local ruminant faeces. | to assess whether flies and slugs acquire strains of campylobacter jejuni and campylobacter coli present in local ruminant faeces. | 2010 | 20337762 |
| immune response to cytolethal distending toxin of aggregatibacter actinomycetemcomitans in periodontitis patients. | cytolethal distending toxin (cdt) is a genotoxin produced by aggregatibacter actinomycetemcomitans. in spite of its association with pathogenesis, little is known about the humoral immune response against the cdt. this study aimed to test whether subgingival colonization and humoral response to a. actinomycetemcomitans would lead to a response against cdt. | 2010 | 20337882 |
| modification of the campylobacter jejuni flagellin glycan by the product of the cj1295 homopolymeric-tract-containing gene. | the campylobacter jejuni flagellin protein is o-glycosylated with structural analogues of the nine-carbon sugar pseudaminic acid. the most common modifications in the c. jejuni 81-176 strain are the 5,7-di-n-acetylated derivative (pse5ac7ac) and an acetamidino-substituted version (pse5am7ac). other structures detected include o-acetylated and n-acetylglutamine-substituted derivatives (pse5am7ac8oac and pse5am7ac8glnnac, respectively). recently, a derivative of pseudaminic acid modified with a di ... | 2010 | 20338909 |
| host factors determine anti-gm1 response following oral challenge of chickens with guillain-barré syndrome derived campylobacter jejuni strain gb11. | anti-ganglioside antibodies with a pathogenic potential are present in c. jejuni-associated guillain-barré syndrome (gbs) patients and are probably induced by molecular mimicry. immunization studies in rabbits and mice have demonstrated that these anti-ganglioside antibodies can be induced using purified lipo-oligosaccharides (los) from c. jejuni in a strong adjuvant. | 2010 | 20339556 |
| growth phase-dependent activation of the dccrs regulon of campylobacter jejuni. | two-component systems are widespread prokaryotic signal transduction devices which allow the regulation of cellular functions in response to changing environmental conditions. the two-component system dccrs (cj1223c-cj1222c) of campylobacter jejuni is important for the colonization of chickens. here, we dissect the dccrs system in more detail and provide evidence that the sensor dccs selectively phosphorylates the cognate effector, dccr. microarray expression profiling, real-time reverse transcr ... | 2010 | 20348251 |
| the internal transcribed spacer region, a new tool for use in species differentiation and delineation of systematic relationships within the campylobacter genus. | the campylobacter genus consists of a number of important human and animal pathogens. although the 16s rrna gene has been used extensively for detection and identification of campylobacter species, there is currently limited information on the 23s rrna gene and the internal transcribed spacer (its) region that lies between the 16s and 23s rrna genes. we examined the potential of the 23s rrna gene and the its region to be used in species differentiation and delineation of systematic relationships ... | 2010 | 20348308 |
| a review of the clinical presentation of dientamoebiasis. | among 750 symptomatic and asymptomatic patients, dientamoeba fragilis was detected at a prevalence of 5.2% and more common than giardia intestinalis. most infected patients presented with diarrhea and abdominal pain with symptoms greater than 2 weeks duration being common. bacterial and viral causes of infection were excluded by routine microbiological techniques. treatment of d. fragilis infection with either iodoquinol, paromomycin, or combination therapy resulted in the eradication of the par ... | 2010 | 20348509 |
| altered linkage of hydroxyacyl chains in lipid a of campylobacter jejuni reduces tlr4 activation and antimicrobial resistance. | modification of the lipid a moiety of bacterial lipopolysaccharide influences cell wall properties, endotoxic activity, and bacterial resistance to antimicrobial peptides. known modifications are variation in the number or length of acyl chains and/or attached phosphoryl groups. here we identified two genes (gnna and gnnb) in the major foodborne pathogen campylobacter jejuni that enable the synthesis of a glcn3n precursor udp 2-acetamido-3-amino-2,3-dideoxy-alpha-d-glucopyranose (udp-glcnac3n) i ... | 2010 | 20351099 |
| the effect of clinical outbreaks of salmonellosis on the prevalence of fecal salmonella shedding among dairy cattle in new york. | the objective of this study was to determine if the within-herd prevalence of fecal salmonella shedding is higher in dairy herds with clinical outbreaks of disease, as compared to herds with subclinical infections only. data were collected prospectively from dairy herds throughout new york that had at least 150 lactating cows and that received clinical service from participating veterinarians. after enrollment, salmonella surveillance consisted of both environmental screening and disease monitor ... | 2010 | 20353290 |
| evidence for a lineage of virulent bacteriophages that target campylobacter. | our understanding of the dynamics of genome stability versus gene flux within bacteriophage lineages is limited. recently, there has been a renewed interest in the use of bacteriophages as 'therapeutic' agents; a prerequisite for their use in such therapies is a thorough understanding of their genetic complement, genome stability and their ecology to avoid the dissemination or mobilisation of phage or bacterial virulence and toxin genes. campylobacter, a food-borne pathogen, is one of the organi ... | 2010 | 20353581 |
| enteritis caused by campylobacter jejuni followed by acute motor axonal neuropathy: a case report. | abstract: | 2010 | 20356396 |
| genetic diversity in campylobacter jejuni is associated with differential colonization of broiler chickens and c57bl/6j il10-deficient mice. | previous studies have demonstrated that campylobacter jejuni, the leading causative agent of bacterial food-borne disease in the usa, exhibits high-frequency genetic variation that is associated with changes in cell-surface antigens and ability to colonize chickens. to expand our understanding of the role of genetic diversity in the disease process, we analysed the ability of three c. jejuni human disease isolates (strains 11168, 33292 and 81-176) and genetically marked derivatives to colonize r ... | 2010 | 20360176 |
| culture-independent techniques applied to food industry water surveillance--a case study. | culture-independent techniques were used for the detection of pathogenic bacteria in drinking water at potentially critical control points along the production lines at a german dairy company and a spanish dry cured ham company. denaturing gradient gel electrophoresis (dgge) was used to describe bacterial population shifts indicating biological instability in the drinking water samples. autochthonous bacteria were identified by sequencing the excised dgge dna bands. more specifically, real-time ... | 2010 | 20363042 |
| genetic, structural, and antigenic analyses of glycan diversity in the o-linked protein glycosylation systems of human neisseria species. | bacterial capsular polysaccharides and lipopolysaccharides are well-established ligands of innate and adaptive immune effectors and often exhibit structural and antigenic variability. although many surface-localized glycoproteins have been identified in bacterial pathogens and symbionts, it not clear if and how selection impacts associated glycoform structure. here, a systematic approach was devised to correlate gene repertoire with protein-associated glycoform structure in neisseria species imp ... | 2010 | 20363948 |
| antimicrobial resistance, virulence, and genotypic profile comparison of campylobacter jejuni and campylobacter coli isolated from humans and retail meats. | a total of 360 spatially and temporally related campylobacter isolates, including 168 from clinical human cases (campylobacter jejuni n = 148; campylobacter coli n = 20) and 192 from retail meats (c. jejuni n = 114; c. coli n = 78), were analyzed for antimicrobial susceptibilities, virulence, and genotypic profiles. ciprofloxacin-resistant c. jejuni was observed in 13.5% and 19% of the isolates from humans and retail chicken breasts, respectively. antimicrobial resistance to ciprofloxacin and er ... | 2010 | 20367499 |
| coupled amino acid deamidase-transport systems essential for helicobacter pylori colonization. | in addition to their classical roles as carbon or nitrogen sources, amino acids can be used for bacterial virulence, colonization, or stress resistance. we found that original deamidase-transport systems impact colonization by helicobacter pylori, a human pathogen associated with gastric pathologies, including adenocarcinoma. we demonstrated that l-asparaginase (hp-ansb) and gamma-glutamyltranspeptidase (hp-gammagt) are highly active periplasmic deamidases in h. pylori, producing ammonia and asp ... | 2010 | 20368342 |
| salmonella enterica serovar typhimurium invades fibroblasts by multiple routes differing from the entry into epithelial cells. | fibroblasts are ubiquitous cells essential to tissue homeostasis. despite their nonphagocytic nature, fibroblasts restrain replication of intracellular bacterial pathogens such as salmonella enterica serovar typhimurium. the extent to which the entry route of the pathogen determines this intracellular response is unknown. here, we analyzed s. typhimurium invasion in fibroblasts obtained from diverse origins, including primary cultures and stable nontransformed cell lines derived from normal tiss ... | 2010 | 20368348 |
| structural determination and daily variations of porcine milk oligosaccharides. | free milk oligosaccharides (os) are major components of mammalian milk. swine are important agricultural species and biomedical models. despite their importance, little is known of the os profile of porcine milk. herein, the porcine milk glycome was elucidated and monitored over the entire lactation period by liquid chromatography profiling and structural determination with mass spectrometry. milk was collected from second-parity sows (n = 3) at farrowing and on days 1, 4, 7, and 24 of lactation ... | 2010 | 20369835 |
| prevalence and antimicrobial resistance of campylobacter species isolated from chicken carcasses during processing in iran. | the objective of this study was to determine the prevalence and antimicrobial resistance of campylobacter spp. isolated from chicken carcasses during different stages of broiler processing in a major commercial poultry processing plant in southwestern iran. overall, 84 chicken carcasses were sampled from 4 sites along the processing line during a total of 7 visits. in addition, 14 water samples from the chiller tank were taken. using the cultural method, 186 of 336 (55.4%) carcasses were positiv ... | 2010 | 20371855 |
| the hp0256 gene product is involved in motility and cell envelope architecture of helicobacter pylori. | helicobacter pylori is the causative agent for gastritis, and peptic and duodenal ulcers. the bacterium displays 5-6 polar sheathed flagella that are essential for colonisation and persistence in the gastric mucosa. the biochemistry and genetics of flagellar biogenesis in h. pylori has not been fully elucidated. bioinformatics analysis suggested that the gene hp0256, annotated as hypothetical, was a flij homologue. in salmonella, flij is a chaperone escort protein for flgn and flit, two proteins ... | 2010 | 20377912 |
| prevalence and biomolecular characterization of campylobacter spp. isolated from retail meat. | we estimated the prevalence of campylobacter spp. in retail meat (n = 352 samples; 104 chicken, 106 pork, and 142 beef) collected in campobasso, italy, comparing two microbiological methods. all the isolates were characterized by biomolecular techniques for epidemiological purposes. campylobacter isolation was performed by selective culture and membrane filtration methods. phenotypic and genotypic methods for genus and species identification were evaluated together with antimicrobial resistance ... | 2010 | 20377962 |
| exposure of campylobacter jejuni to 6 degrees c: effects on heat resistance and electron transport activity. | human infection with campylobacter jejuni is frequently associated with the consumption of foods, especially chicken meat, which have been exposed to a range of temperatures during processing, storage, and cooking. despite the public health importance of c. jejuni, little is known about the effects of cold exposure (refrigeration) on the subsequent ability of this pathogen to survive heat challenge. this work examined the effect of rapid exposure to 6 degrees c for 24 h on the heat resistance at ... | 2010 | 20377963 |
| an overview of methods used to clarify pathogenesis mechanisms of campylobacter jejuni. | thermotolerant campylobacters are the most frequent cause of bacterial infection of the lower intestine worldwide. the mechanism of pathogenesis of campylobacter jejuni comprises four main stages: adhesion to intestinal cells, colonization of the digestive tract, invasion of targeted cells, and toxin production. in response to the high number of cases of human campylobacteriosis, various virulence study models are available according to the virulence stage being analyzed. the aim of this review ... | 2010 | 20377972 |
| role of the feob protein and siderophore in promoting virulence of xanthomonas oryzae pv. oryzae on rice. | xanthomonas oryzae pv. oryzae causes bacterial blight, a serious disease of rice. our analysis revealed that the x. oryzae pv. oryzae genome encodes genes responsible for iron uptake through feob (homolog of the major bacterial ferrous iron transporter) and a siderophore. a mutation in the x. oryzae pv. oryzae feob gene causes severe virulence deficiency, growth deficiency in iron-limiting medium, and constitutive production of a siderophore. we identified an iron regulated xss gene cluster, in ... | 2010 | 20382771 |
| activation of pmra inhibits lpxt-dependent phosphorylation of lipid a promoting resistance to antimicrobial peptides. | during its transport to the bacterial surface, the phosphate groups of the lipid a anchor of escherichia coli and salmonella lipopolysaccharide are modified by membrane enzymes including arnt, epta and lpxt. arnt and epta catalyse the periplasmic addition of the positively charged substituents 4-amino-4-deoxy-l-arabinose and phosphoethanolamine respectively. these modifications are controlled by the pmra transcriptional regulator and confer resistance to cationic antimicrobial peptides, includin ... | 2010 | 20384697 |
| the caenorhabditis elegans bus-2 mutant reveals a new class of o-glycans affecting bacterial resistance. | microbacterium nematophilum causes a deleterious infection of the c. elegans hindgut initiated by adhesion to rectal and anal cuticle. c. elegans bus-2 mutants, which are resistant to m. nematophilum and also to the formation of surface biofilms by yersinia sp., carry genetic lesions in a putative glycosyltransferase containing conserved domains of core-1 beta1,3-galactosyltransferases. bus-2 is predicted to act in the synthesis of core-1 type o-glycans. this observation implies that the infecti ... | 2010 | 20385555 |
| cytolethal distending toxin family members are differentially affected by alterations in host glycans and membrane cholesterol. | cytolethal distending toxins (cdts) are tripartite protein exotoxins produced by a diverse group of pathogenic gram-negative bacteria. based on their ability to induce dna damage, cell cycle arrest, and apoptosis of cultured cells, cdts are proposed to enhance virulence by blocking cellular division and/or directly killing epithelial and immune cells. despite the widespread distribution of cdts among several important human pathogens, our understanding of how these toxins interact with host cell ... | 2010 | 20385557 |
| zinc uptake contributes to motility and provides a competitive advantage to proteus mirabilis during experimental urinary tract infection. | proteus mirabilis, a gram-negative bacterium, represents a common cause of complicated urinary tract infections in catheterized patients or those with functional or anatomical abnormalities of the urinary tract. znub, the membrane component of the high-affinity zinc (zn(2+)) transport system znuacb, was previously shown to be recognized by sera from infected mice. since this system has been shown to contribute to virulence in other pathogens, its role in proteus mirabilis was investigated by con ... | 2010 | 20385754 |
| indoleamine 2,3-dioxygenase 1 is a lung-specific innate immune defense mechanism that inhibits growth of francisella tularensis tryptophan auxotrophs. | upon microbial challenge, organs at various anatomic sites of the body employ different innate immune mechanisms to defend against potential infections. accordingly, microbial pathogens evolved to subvert these organ-specific host immune mechanisms to survive and grow in infected organs. francisella tularensis is a bacterium capable of infecting multiple organs and thus encounters a myriad of organ-specific defense mechanisms. this suggests that f. tularensis may possess specific factors that ai ... | 2010 | 20385761 |
| a timescale for evolution, population expansion, and spatial spread of an emerging clone of methicillin-resistant staphylococcus aureus. | due to the lack of fossil evidence, the timescales of bacterial evolution are largely unknown. the speed with which genetic change accumulates in populations of pathogenic bacteria, however, is a key parameter that is crucial for understanding the emergence of traits such as increased virulence or antibiotic resistance, together with the forces driving pathogen spread. methicillin-resistant staphylococcus aureus (mrsa) is a common cause of hospital-acquired infections. we have investigated an mr ... | 2010 | 20386717 |
| detection of a mixed infection in a culture-negative brain abscess by broad-spectrum bacterial 16s rrna gene pcr. | we describe the identification of two bacterial pathogens from a culture-negative brain abscess by the use of broad-spectrum 16s rrna gene pcr. simultaneous detection of fusobacterium nucleatum and porphyromonas endodontalis was possible due to a 24-bp length difference of their partially amplified 16s rrna genes, which allowed separation by high-resolution polyacrylamide gel electrophoresis. | 2010 | 20392909 |
| consumption of human milk oligosaccharides by gut-related microbes. | human milk contains large amounts of complex oligosaccharides that putatively modulate the intestinal microbiota of breast-fed infants by acting as decoy binding sites for pathogens and as prebiotics for enrichment of beneficial bacteria. several bifidobacterial species have been shown to grow well on human milk oligosaccharides. however, few data exist on other bacterial species. this work examined 16 bacterial strains belonging to 10 different genera for growth on human milk oligosaccharides. ... | 2010 | 20394371 |
| ivig treatment and prognosis in guillain-barré syndrome. | guillain-barré syndrome (gbs) is an acute, immune-mediated polyneuropathy that often leads to severe weakness. intravenous immunoglobulin (ivig) is a proven effective treatment for gbs (class 1 evidence). however, about 25% of patients need artificial ventilation and 20% are still unable to walk unaided after 6 months. important clinical factors associated with poor outcome are age, presence of preceding diarrhea and the severity of disability in the early course of disease. these clinical facto ... | 2010 | 20396937 |
| amino-terminal residues dictate the export efficiency of the campylobacter jejuni filament proteins via the flagellum. | bacterial flagella play an essential role in the pathogenesis of numerous enteric pathogens. the flagellum is required for motility, colonization, and in some instances, for the secretion of effector proteins. in contrast to the intensively studied flagella of escherichia coli and salmonella typhimurium, the flagella of campylobacter jejuni, helicobacter pylori and vibrio cholerae are less well characterized and composed of multiple flagellin subunits. this study was performed to gain a better u ... | 2010 | 20398207 |
| high taxonomic level fingerprint of the human intestinal microbiota by ligase detection reaction--universal array approach. | affecting the core functional microbiome, peculiar high level taxonomic unbalances of the human intestinal microbiota have been recently associated with specific diseases, such as obesity, inflammatory bowel diseases, and intestinal inflammation. | 2010 | 20398430 |
| host-interactive genes in amerindian helicobacter pylori diverge from their old world homologs and mediate inflammatory responses. | helicobacter pylori is the dominant member of the gastric microbiota and has been associated with an increased risk of gastric cancer and peptic ulcers in adults. h. pylori populations have migrated and diverged with human populations, and health effects vary. here, we describe the whole genome of the cag-positive strain v225d, cultured from a venezuelan piaroa amerindian subject. to gain insight into the evolution and host adaptation of this bacterium, we undertook comparative h. pylori genomic ... | 2010 | 20400544 |
| investigation of the presence and protective effects of maternal antibodies against campylobacter jejuni in chickens. | the role of maternal antibodies in the lag phase of campylobacter positivity, widely observed in commercial broiler flocks, was investigated. the results indicate that 3-wk-old birds derived from a commercial flock are more susceptible to colonization with campylobacter jejuni than 1-to-2-wk-old birds. this increasing susceptibility parallels the loss of maternally derived, circulating, anti-campylobacter, immunoglobulin y antibodies as detected by enzyme-linked immunosorbent assay. the role of ... | 2010 | 20408404 |
| occurrence of thermotolerant campylobacter species in surface waters of a mediterranean area and in its prevailing pollution sources. | to determine the prevalence of campylobacter in surface waters of a highly populated mediterranean area. | 2010 | 20408911 |
| selecting for development of fluoroquinolone resistance in a campylobacter jejuni strain 81116 in chickens using various enrofloxacin treatment protocols. | to determine the effect of various enrofloxacin dose regimes on the colonization and selection of resistance in campylobacter jejuni strain 81116p in experimentally colonized chickens. | 2010 | 20408918 |
| the occurrence of campylobacter in river water and waterfowl within a watershed in southern ontario, canada. | quantitative pcr and a culture method were used to investigate campylobacter occurrence over 3 years in a watershed located in southern ontario, canada that is used as a source of drinking water. | 2010 | 20408936 |
| tropheryma whipplei in children with gastroenteritis. | tropheryma whipplei, which causes whipple disease, is found in human feces and may cause gastroenteritis. to show that t. whipplei causes gastroenteritis, pcrs for t. whipplei were conducted with feces from children 2-4 years of age. western blotting was performed for samples from children with diarrhea who had positive or negative results for t. whipplei. t. whipplei was found in samples from 36 (15%) of 241 children with gastroenteritis and associated with other diarrheal pathogens in 13 (33%) ... | 2010 | 20409366 |
| nursing our microbiota: molecular linkages between bifidobacteria and milk oligosaccharides. | as the sole nutrition provided to infants, bioactive molecules dissolved in milk influence the development of our gut microbiota. accordingly, human milk oligosaccharides (hmos) are minimally digested by the infant and persist to negatively and positively regulate gut microbiota. infant-type bifidobacteria utilize these soluble carbohydrate oligomers by convergent mechanisms. bifidobacterium longum subsp. infantis efficiently consumes several small mass hmos and possesses a large gene cluster an ... | 2010 | 20409714 |
| a membrane-located glycosyltransferase complex required for biosynthesis of the d-galactan i lipopolysaccharide o antigen in klebsiella pneumoniae. | d-galactan i is a polysaccharide with the disaccharide repeat unit structure [-->3-beta-d-galf-(1-->3)-alpha-d-galp-(1-->]. this glycan represents the lipopolysaccharide o antigen found in many gram-negative bacteria, including several klebsiella pneumoniae o serotypes. the polysaccharide is synthesized in the cytoplasm prior to its export via an atp-binding cassette transporter. sequence analysis predicts three galactosyltransferases in the d-galactan i genetic locus. they are wbbo (belonging t ... | 2010 | 20410291 |
| helicobacter pylori induces erk-dependent formation of a phospho-c-fos c-jun activator protein-1 complex that causes apoptosis in macrophages. | macrophages are essential components of innate immunity, and apoptosis of these cells impairs mucosal defense to microbes. helicobacter pylori is a gastric pathogen that infects half of the world population and causes peptic ulcer disease and gastric cancer. the host inflammatory response fails to eradicate the organism. we have reported that h. pylori induces apoptosis of macrophages by generation of polyamines from ornithine decarboxylase (odc), which is dependent on c-myc as a transcriptional ... | 2010 | 20410304 |
| impact of pseudomonas aeruginosa genomic instability on the application of typing methods for chronic cystic fibrosis infections. | the liverpool epidemic strain (les) of pseudomonas aeruginosa is widespread among cystic fibrosis (cf) patients in the united kingdom and has emerged recently in north america. in this study, we report the analysis of 24 "anomalous" cf isolates of p. aeruginosa that produced inconsistent results with regard to either pulsed-field gel electrophoresis (pfge) or pcr tests for the les. we used a new typing method, the arraytube genotyping system, to determine that of the 24 anomalous isolates tested ... | 2010 | 20410349 |
| detection of campylobacter jejuni by culture and real-time pcr in a french cohort of patients with guillain-barre syndrome. | bacteriological culture and real-time pcr (rt-pcr) were used to detect campylobacter jejuni in fecal samples from a french cohort of 237 patients with guillain-barré syndrome (gbs). we provide evidence that diverse serotypes and genotypes of c. jejuni are a major trigger of gbs in france. | 2010 | 20410350 |
| erythema nodosum associated with salmonella enteritidis. | erythema nodosum (en) is the most frequent type of panniculitis in childhood. although frequently idiopathic, it may be associated with a wide variety of conditions ranging from infections, to sarcoidosis, to collagen vascular diseases to drugs. | 2010 | 20411062 |
| quantitative analysis of viable, stressed and dead cells of campylobacter jejuni strain 81-176. | campylobacter jejuni is an important foodborne gastrointestinal pathogen and highly sensitive to environmental stresses. research has shown that changes in culturability, cell morphology, and viability occur when c. jejuni cells are subjected to stresses. in this study, real-time pcr, ethidium monoazide (ema) in combination with real-time pcr (ema-pcr), baclight bacterial viability staining, and agar plate counting methods were used to quantitatively analyze viable, stressed, and dead c. jejuni ... | 2010 | 20417391 |
| killing of bacteria by copper surfaces involves dissolved copper. | bacteria are rapidly killed on copper surfaces. however, the mechanism of this process remains unclear. using enterococcus hirae, the effect of inactivation of copper homeostatic genes and of medium compositions on survival and copper dissolution was tested. the results support a role for dissolved copper ions in killing. | 2010 | 20418419 |
| multiplex identification of microbes. | we have adapted molecular inversion probe technology to identify microbes in a highly multiplexed procedure. this procedure does not require growth of the microbes. rather, the technology employs dna homology twice: once for the molecular probe to hybridize to its homologous dna and again for the 20-mer oligonucleotide barcode on the molecular probe to hybridize to a commercially available molecular barcode array. as proof of concept, we have designed, tested, and employed 192 molecular probes f ... | 2010 | 20418427 |
| adhesion ability of campylobacter jejuni to ht-29 cells increases with the augmentation of oxidant agent concentration. | campylobacter jejuni (c. jejuni) is a leading cause of human enteritis worldwide and the most frequently reported zoonotic agent in the european union. despite the fact that c. jejuni is a microaerobic bacteria, known as a fragile one, it is able to survive through adverse conditions such as oxidative stress. the purpose of this study was first to test the oxidative stress resistance in 22 c. jejuni strains of various origins, and to compare adhesive and invasive abilities of four selected strai ... | 2010 | 20419374 |
| detailed in vivo analysis of the role of helicobacter pylori fur in colonization and disease. | helicobacter pylori persistently colonizes the harsh and dynamic environment of the stomach in over one-half of the world's population and has been identified as a causal agent in a spectrum of pathologies that range from gastritis to invasive adenocarcinoma. the ferric uptake regulator (fur) is one of the few regulatory proteins that has been identified in h. pylori. fur regulates genes important for acid acclimation and oxidative stress and has been shown to be important for colonization of h. ... | 2010 | 20421381 |
| characterization of the specific interaction between sialoadhesin and sialylated campylobacter jejuni lipooligosaccharides. | in campylobacter jejuni-induced guillain-barré syndrome (gbs), molecular mimicry between c. jejuni lipooligosaccharide (los) and host gangliosides leads to the production of cross-reactive antibodies directed against the peripheral nerves of the host. currently, the presence of surface exposed sialylated los in c. jejuni is the single known bacterial pathogenesis factor associated with the development of gbs. using a unique, well-characterized strain collection, we demonstrate that gbs-associate ... | 2010 | 20421384 |
| molecular structure of wlbb, a bacterial n-acetyltransferase involved in the biosynthesis of 2,3-diacetamido-2,3-dideoxy-d-mannuronic acid . | the pathogenic bacteria pseudomonas aeruginosa and bordetella pertussis contain in their outer membranes the rare sugar 2,3-diacetamido-2,3-dideoxy-d-mannuronic acid. five enzymes are required for the biosynthesis of this sugar starting from udp-n-acetylglucosamine. one of these, referred to as wlbb, is an n-acetyltransferase that converts udp-2-acetamido-3-amino-2,3-dideoxy-d-glucuronic acid (udp-glcnac3na) to udp-2,3-diacetamido-2,3-dideoxy-d-glucuronic acid (udp-glcnac3naca). here we report t ... | 2010 | 20433200 |
| survival of campylobacter jejuni on raw chicken legs packed in high-oxygen or high-carbon dioxide atmosphere after the decontamination with lactic acid/sodium lactate buffer. | quantitative risk assessment studies performed elsewhere showed the importance of reducing counts of campylobacter jejuni on chicken carcasses for decrease of incidence of human campylobacteriosis. the current study indicated that 1.8 log cfu/g reduction of inoculated c. jejuni (6 log cfu/g) can be achieved by decontamination with lactic acid buffered with sodium lacatate (la/nala, 10% w/v, ph 3.0). subsequent packaging under modified atmosphere of 80% o(2)/20%n(2) resulted in additional reducti ... | 2010 | 20434228 |
| a rapid protein folding assay for the bacterial periplasm. | an array of genetic screens and selections has been developed for reporting protein folding and solubility in the cytoplasm of living cells. however, there are currently no analogous folding assays for the bacterial periplasm, despite the significance of this compartment for the expression of recombinant proteins, especially those requiring important posttranslational modifications (e.g., disulfide bond formation). here, we describe an engineered genetic selection for monitoring protein folding ... | 2010 | 20440843 |
| n-linked glycoengineering for human therapeutic proteins in bacteria. | approx. 70% of human therapeutic proteins are n-linked glycoproteins, and therefore host cells for production must contain the relevant protein modification machinery. the discovery and characterisation of the n-linked glycosylation pathway in the pathogenic bacterium campylobacter jejuni, and subsequently its functional transfer to escherichia coli, presents the opportunity of using prokaryotes as cell factories for therapeutic protein production. not only could bacteria reduce costs and increa ... | 2010 | 20449632 |
| extracellular hsp90 serves as a co-factor for mapk activation and latent viral gene expression during de novo infection by kshv. | the kaposi's sarcoma-associated herpesvirus (kshv) is the causative agent of kaposi's sarcoma (ks), an important cause of morbidity and mortality in immunocompromised patients. kshv interaction with the cell membrane triggers activation of specific intracellular signal transduction pathways to facilitate virus entry, nuclear trafficking, and ultimately viral oncogene expression. extracellular heat shock protein 90 localizes to the cell surface (cshsp90) and facilitates signal transduction in can ... | 2010 | 20451233 |
| characterization of probiotic strains: an application as feed additives in poultry against campylobacter jejuni. | campylobacteriosis is at present the most frequent zoonosis in humans and the main source is poultry meat contaminated by campylobacter jejuni. an alternative and effective approach to antibiotic administration to livestock to reduce bacterial contamination is the use of probiotics, which can help to improve the natural defence of animals against pathogenic bacteria. in this study 55 lactic acid bacteria and bifidobacteria were screened for desirable properties for their application as probiotic ... | 2010 | 20452074 |
| highly discriminatory single-nucleotide polymorphism interrogation of escherichia coli by use of allele-specific real-time pcr and eburst analysis. | in total, 782 escherichia coli strains originating from various host sources have been analyzed in this study by using a highly discriminatory single-nucleotide polymorphism (snp) approach. a set of eight snps, with a discrimination value (simpson's index of diversity [d]) of 0.96, was determined using the minimum snps software, based on sequences of housekeeping genes from the e. coli multilocus sequence typing (mlst) database. allele-specific real-time pcr was used to screen 114 e. coli isolat ... | 2010 | 20453128 |
| increase in acid tolerance of campylobacter jejuni through coincubation with amoebae. | campylobacter jejuni is a recognized and common gastrointestinal pathogen in most parts of the world. human infections are often food borne, and the bacterium is frequent among poultry and other food animals. however, much less is known about the epidemiology of c. jejuni in the environment and what mechanisms the bacterium depends on to tolerate low ph. the sensitive nature of c. jejuni stands in contrast to the fact that it is difficult to eradicate from poultry production, and even more contr ... | 2010 | 20453130 |
| association study between an outbreak of guillain-barre syndrome in jilin, china, and preceding campylobacter jejuni infection. | from june to july 2007, 36 cases of guillain-barre syndrome (gbs) occurred in a township in north china. serological study and bacteria culture were performed to investigate the association between preceding campylobacter jejuni infection and this gbs outbreak. anti-c. jejuni antibodies were found in significantly higher numbers of gbs patients (igm 84%, igg 87.5%) than in healthy inspection cases (igm 33%, igg 27%). igg anti-gm1 was the dominant anti-ganglioside antibody among the gbs patients. ... | 2010 | 20455754 |
| nucleic acid sensing receptors in systemic lupus erythematosus: development of novel dna- and/or rna-like analogues for treating lupus. | double-stranded (ds) dna, dna- or rna-associated nucleoproteins are the primary autoimmune targets in sle, yet their relative inability to trigger similar autoimmune responses in experimental animals has fascinated scientists for decades. while many cellular proteins bind non-specifically negatively charged nucleic acids, it was discovered only recently that several intracellular proteins are involved directly in innate recognition of exogenous dna or rna, or cytosol-residing dna or rna viruses. ... | 2010 | 20456414 |
| overexpression of the reca gene decreases oral but not intraperitoneal fitness of salmonella enterica. | transcription of the salmonella enterica reca gene is negatively controlled by the lexa protein, the repressor of the sos response. the introduction of a mutation (recao6869) in the lexa binding site, in the promoter region of the s. enterica atcc 14028 reca gene, allowed the analysis of the effect that reca protein overproduction has on the fitness of this virulent strain. the fitness of orally but not intraperitoneally inoculated recao6869 cells decreased dramatically. however, the sos respons ... | 2010 | 20457791 |
| systematic review and meta-analysis of saccharomyces boulardii in adult patients. | this article reviews the evidence for efficacy and safety of saccharomyces boulardii (s. boulardii) for various disease indications in adults based on the peer-reviewed, randomized clinical trials and pre-clinical studies from the published medical literature (medline, clinical trial websites and meeting abstracts) between 1976 and 2009. for meta-analysis, only randomized, blinded controlled trials unrestricted by language were included. pre-clinical studies, volunteer studies and uncontrolled s ... | 2010 | 20458757 |
| intestinal mucus protects campylobacter jejuni in the ceca of colonized broiler chickens against the bactericidal effects of medium-chain fatty acids. | campylobacter jejuni is the most common cause of bacterial-mediated diarrheal disease worldwide. because poultry and poultry products are a major source of c. jejuni infections in humans, efforts should be taken to develop strategies to decrease campylobacter shedding during primary production. for this purpose, the efficacy of medium-chain fatty acids (mcfa) as feed additives to control c. jejuni colonization in broiler chickens was analyzed. first, the antimicrobial activity of the mcfa caproi ... | 2010 | 20460660 |
| cytochrome p450 2d6 as a model antigen. | cytochrome p450 2d6 (cyp2d6) has been identified as the major autoantigen in type 2 autoimmune hepatitis (aih). however, because of a lack of appropriate animal models, the etiology of aih is still poorly understood. we generated a mouse model for aih using the human cyp2d6 as a triggering molecule for autoimmunity. we infected wild-type fvb mice with an adenovirus expressing human cyp2d6 (ad-2d6) to break self-tolerance to the mouse cyp2d6 homologues. ad-2d6-infected mice showed persistent feat ... | 2010 | 20460894 |
| purified chicken intestinal mucin attenuates campylobacter jejuni pathogenicity in vitro. | campylobacter jejuni is a major causative agent of diarrhoeal disease worldwide in the human population. in contrast, heavy colonization of poultry typically does not lead to disease and colonized chickens are a major source of campylobacter infections in humans. previously, we have shown that chicken (but not human) intestinal mucus inhibits c. jejuni internalization. in this study, we test the hypothesis that chicken mucin, the main component of mucus, is responsible for this inhibition of c. ... | 2010 | 20466838 |
| variation among genome sequences of h37rv strains of mycobacterium tuberculosis from multiple laboratories. | the publication of the complete genome sequence for mycobacterium tuberculosis h37rv in 1998 has had a great impact on the research community. nonetheless, it is suspected that genetic differences have arisen in stocks of h37rv that are maintained in different laboratories. in order to assess the consistency of the genome sequences among h37rv strains in use and the extent to which they have diverged from the original strain sequenced, we carried out whole-genome sequencing on six strains of h37 ... | 2010 | 20472797 |