Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| temporomandibular joint involvement caused by borrelia burgdorferi. | lyme borreliosis is an endemic disease in slovenia with an incidence of around 150 patients per 100,000 inhabitants. although the large joints are most typically affected in lyme borreliosis, there are also periods of disease activity with arthritis or arthralgias involving smaller joints, including the temporo-mandibular joint. | 2007 | 17942315 |
| detection and identification of borrelia burgdorferi sensu lato genospecies in ticks from three different regions in slovakia. | lyme borreliosis is one of the most common tick-borne diseases that occur in slovakia. in this study, borrelia burgdorferi sensu lato was detected and cultivated from questing ticks collected in three areas of slovakia. two methods, restriction fragment length polymorphism and reverse line blot, were used for identification of isolates and determination of the prevalence of b. burgdorferi s.l. in the ticks. the prevalence of b. burgdorferi s.l. in i. ricinus detected by reverse line blot was 31. ... | 2007 | 17943405 |
| decline in the frequencies of borrelia burgdorferi ospa161 175-specific t cells after antibiotic therapy in hla-drb1*0401-positive patients with antibiotic-responsive or antibiotic-refractory lyme arthritis. | synovitis in patients with antibiotic-refractory lyme arthritis persists for months to several years after antibiotic therapy. this course, which may result from infection-induced autoimmunity, is associated with t cell recognition of borrelia burgdorferi outer surface protein a (ospa(161-175)) and with hla-dr molecules that bind this epitope, including the drb1*0401 molecule. in this study, we used tetramer reagents to determine the frequencies of ospa(161-175)-specific t cells in samples of pb ... | 2007 | 17947711 |
| acute respiratory distress syndrome in persons with tickborne relapsing fever--three states, 2004-2005. | tickborne relapsing fever (tbrf) is a bacterial illness caused by certain species of borrelia and transmitted through brief and painless bites from ornithodoros ticks. illness usually is characterized by intermittent periods of fever, fatigue, and muscle aches. in april 2005, cdc received reports of two cases of severe tbrf associated with acute respiratory distress syndrome (ards) in residents of california and nevada. after a report describing these cases was posted on cdc's epidemic informati ... | 2007 | 17947965 |
| [epidemiology of borreliosis in workers of the district forestry offices in lower silesia]. | the work presents epidemiological analysis of borrelia burgdorferi (b.b.) infections and borreliosis cases in workers of the district forestry offices in lower silesia in 2003-2005. the infections rate was proved to be high and increasing in time. in 2003-2005 the presence of b.b. antibodies was noted in 672 (35%) examined people. in particular district forestry offices the rate of positive results was between 19.42-50.50%. in several district forestry offices a marked increase of b.b. infection ... | 2007 | 17956058 |
| a novel leptospiral protein increases icam-1 and e-selectin expression in human umbilical vein endothelial cells. | it has been reported previously that activation of vascular endothelium by outer membrane proteins of the spirochetes borrelia sp. and treponema sp. resulted in enhanced expression of endothelial cell adhesion molecules. to investigate the role of leptospiral proteins in this process, a predicted lipoprotein encoded by the gene lic10365 was selected, which belongs to a paralogous family that presents a domain of unknown function, duf1565. the lic10365 gene was cloned and the protein expressed in ... | 2007 | 17956423 |
| zinc is the metal cofactor of borrelia burgdorferi peptide deformylase. | peptide deformylase (pdf, e.c. 3.5.1.88) catalyzes the removal of n-terminal formyl groups from nascent ribosome-synthesized polypeptides. pdf contains a catalytically essential divalent metal ion, which is tetrahedrally coordinated by three protein ligands (his, his, and cys) and a water molecule. previous studies revealed that the metal cofactor is a fe2+ ion in escherichia coli and many other bacterial pdfs. in this work, we found that pdfs from two iron-deficient bacteria, borrelia burgdorfe ... | 2007 | 17977509 |
| [lyme carditis--a bitter lesson or a delayed diagnostic success--a case report]. | lyme carditis is a well known disorder; however, its diagnosis still remains a challenge because of varied clinical picture, low incidence rate and difficulties in detection of the aetiological agent (borrelia burgdorferi). we report a case of a 60-year-old man with a 2.5-year history of dilated cardiomyopathy, recurring episodes of acute heart failure and arrhythmias which finally were diagnosed as lyme carditis. the diagnosis was confirmed by endomyocardial biopsy that revealed spirochetes as ... | 2007 | 17979052 |
| lyme borreliosis incidence in two french departments: correlation with infection of ixodes ricinus ticks by borrelia burgdorferi sensu lato. | we conducted a prospective study to estimate the lyme borreliosis incidence in two rural french departments, meuse and puy-de-dôme. concurrently, we investigated the prevalence of ticks infected with borrelia burgdorferi sensu lato (sl) and anaplasma phagocytophilum. the incidence of lyme borreliosis decreased from 156 to 109/100,000 inhabitants in meuse and from 117 to 76/100,000 inhabitants in puy-de-dôme in 2004 and 2005, respectively, corresponding to a decrease in the density of ixodes rici ... | 2007 | 17979533 |
| a hard tick relapsing fever group spirochete in a brazilian rhipicephalus (boophilus) microplus. | tick-borne diseases usually comprise a complex epidemiological and ecological network connecting the vector, pathogen, and a group of host species. symptoms associated with lyme disease have been reported in brazil, but no borrelia sp. has been definitively related to these events. here we have identified a b. lonestari/b. theileri-related spirochete dna in the cattle tick rhipicephalus (boophilus) microplus from brazil. four hundred r. microplus and 80 amblyomma cajennense ticks were screened, ... | 2007 | 17979536 |
| a comparison of two dna extraction approaches in the detection of borrelia burgdorferi sensu lato from live ixodes ricinus ticks by pcr and reverse line blotting. | we tested two approaches to extract borrelia dna from live ixodes ricinus ticks before polymerase chain reaction (pcr) and reverse line blotting (rlb): dna extraction of one half of the tick after incubation in bsk medium and dna extraction of the other half of the tick directly, using ammonium hydroxide. among 2079 ticks, 31.2% (n=649) were found to be borrelia-infected by pcr-rlb test using at least one of the dna extraction methods. five hundred four ticks (24.2%) were found infected after in ... | 2007 | 17979538 |
| ticks and associated pathogens collected from domestic animals in the netherlands. | following an outbreak of autochthonous canine babesiosis in the netherlands, a request made to veterinarians and the public to collect ticks from companion animals resulted in 4298 ticks submitted between july 2005 and october 2006 to our center. ticks were identified as ixodes ricinus adults (2907/4298, 67.6%), ixodes sp. nymphs (529/4298, 12.3%) and ixodes sp. larvae (385/4298, 9.0%), i. hexagonus adults (328/4298, 7.6%), dermacentor reticulatus (72/4298, 1.7%), and several other exotic tick s ... | 2007 | 17979540 |
| bradycardic child: what's to blame? | 2007 | 17980807 | |
| spit-acular entry: borrelia gets help from a tick salivary protein to move from the mammalian host to the arthropod vector. | in vector-host-pathogen interactions, vector saliva plays a key role in the successful acquisition of blood and in facilitating the establishment of pathogens in mammalian hosts. in this issue of cell host & microbe, narasimhan et al. describe how a tick salivary antioxidant neutralizes reactive oxygen species at the tick-host interface and facilitates the tick vector's acquisition of borrelia burgdorferi bacteria from an infected mammalian host. | 2007 | 18005711 |
| a tick antioxidant facilitates the lyme disease agent's successful migration from the mammalian host to the arthropod vector. | the tick ixodes scapularis is an efficient vector for microbes, including the lyme disease agent borrelia burgdorferi. ticks engorging on vertebrates induce recruitment of inflammatory cells to the bite site. for efficient transmission to the vector, pathogens have to traffic through this complex feeding site while avoiding the deleterious effects of immune cells. we show that a tick protein, salp25d, plays a critical role-in the mammalian host-for acquisition of borrelia burgdorferi by the vect ... | 2007 | 18005713 |
| effect of proteins from the spirochete borrelia burgdorferi sensu lato on myelinated nerve excitability. | we studied the effect of spirochete borrelia burgdorferi sensu lato cell membrane proteins on excitability of myelinated nerve fiber. it was found that cell surface proteins of spirochetes b. burgdorferi s. s. bind to ranvier nodes of the axon and to schwann cells. binding of b. burgdorferi s. s. and b. garinii to the nerve fiber modulates the amplitude and conduction velocity of the action potential, while b. afzelii had no effect on these parameters. the decrease in the spike amplitude and con ... | 2007 | 18019007 |
| does tick-borne relapsing fever have an animal reservoir in east africa? | tick-borne relapsing fevers (tbrf) are caused by infection with borrelia spirochetes and transmitted to humans by ticks. all except east african tbrf, caused by borrelia duttonii, are known zoonoses. this widespread, endemic and pathogenic infection has only been found in humans and the ornithodoros sp. soft tick vectors. we investigated the role of domestic animals as possible reservoirs of infection in a tbrf endemic region. tick infestations in households and pigpens were investigated in the ... | 2007 | 18021022 |
| surveillance for zoonotic vector-borne infections using sick dogs from southeastern brazil. | for many vector-borne organisms, dogs can be used as sentinels to estimate the risk of human infection. the objective of this study was to use dogs as sentinels for multiple vector-borne organisms in order to evaluate the potential for human infection with these agents in southeastern brazil. blood from 198 sick dogs with clinicopathological abnormalities consistent with tick-borne infections were selected at the são paulo state university veterinary teaching hospital in botucatu and tested for ... | 2007 | 18021025 |
| borrelia burgdorferi sensu lato in siberian chipmunks (tamias sibiricus) introduced in suburban forests in france. | numerous vertebrate reservoirs have been described for borrelia burgdorferi sensu lato (sl), which includes the etiological agents of lyme borreliosis (lb). the siberian chipmunk (tamias sibiricus) is a rodent originating from asia, where it is suspected to be a b. burgdorferi reservoir. it has been intentionally released into the wild in europe since the 1970s, but has not yet been subject to any study regarding its association with the lb agent. in this paper we studied siberian chipmunk infes ... | 2007 | 18021026 |
| detection of spirochetal micro-organisms by focus-floating microscopy in necrobiotic xanthogranuloma. | necrobiotic xanthogranuloma (nxg) is a rare histiocytic disorder of unknown origin. | 2007 | 18021850 |
| fully automated, homogeneous nucleic acid detection technology based on dry-reagent assay chemistry and time-resolved fluorometry. | 2007 | 18030698 | |
| [specifics of cytokine regulation in tick-borne encephalitis and lyme borreliosis]. | the aim of the study was to evaluate the dynamics of t-lymphocyte cytokine profile during the acute period of tick-bore encephalitis (febrile and meningeal forms) and non-erythematous lyme borreliosis (nelb). elisa (vector-best, novosybirsk) and iha (virion, tomsk) techniques were used for laboratory diagnostics of tick-borne encephalitis (tbe). etiological verification of lyme borreliosis (lb) was performed using immune-enzyme test systems for detection of igg and igm antibodies to borellia bur ... | 2007 | 18030714 |
| cutaneous b-cell pseudolymphoma at the site of vaccination. | pseudolymphomas are a rare complication of vaccination, presenting with dense lymphoid infiltrates and prominent follicular pattern. we report our observations on 4 patients with vaccination-induced b-cell pseudolymphoma (all females; age range 19 to 60 years; median: 34.5 years). clinically 3 patients presented with subcutaneous nodules and 1 presented with a large, indurated, erythematous plaque. histology revealed in all cases dense lymphoid infiltrates in the subcutaneous fat with prominent ... | 2007 | 18032948 |
| [acute myelitis and lyme disease]. | acute myelitis accounts for 4 to 5 percent of all cases of neuroborreliosis. in the literature, simultaneous spinal mri and cerebrospinal fluid (csf) investigations are presented for only 8 cases. we describe here 3 cases of acute lyme myelitis. | 2007 | 18033042 |
| malt lymphoma : recent advances in aetiology and molecular genetics. | mucosa-associated lymphoid tissue (malt) lymphoma is a common low grade b-cell lymphoma arising from a background of chronic inflammatory disease at a number of mucosal sites. those originating in the stomach are causatively linked to helicobacter pylori infection and eradication of the bacterium with antibiotics leads to long-term complete regression of the lymphoma in aproximately 70% of cases. now, there is further evidence of linking campylobacter jejuni, borrelia burgdorferi and chlamydia p ... | 2007 | 18040143 |
| [comparison of test with antigen vlse (c6) with tests with recombinant antigens in patients with lyme borreliosis]. | in diagnosis of lyme borreliosis classic, recombinant antigens are used. introduced recombinant antigen vlse increases hope to improve sensibility of the tests. | 2007 | 18044336 |
| [clinical forms of neuroborreliosis among hospitalized patients in the years 2000-2005]. | to evaluate the frequency of clinical forms as well as laboratory and neuroimaging results of patients with diagnosed neuroborreliosis in the years 2000-2005 due to neuroborreliosis. | 2007 | 18044338 |
| [immunopathology of lyme arthritis]. | lyme borreliosis (lyme disease) is the most prevalent tick-borne disease caused by spirochaetes of the borrelia species complex. arthritis is one of the common manifestations of b. burgdorferi infection. the pathomechanism of articular changes in lyme arthritis has not yet been elucidated. histopathological studies of synovia and immunological changes are similar to rheumatoid arthritis. in the early stage of inflammation b. burgdorferi interact with polynuclear granulocytes and epithelial cells ... | 2007 | 18044347 |
| third degree atrioventricular block and accelerated idioventricular rhythm associated with a heart base chemodectoma in a syncopal rottweiler. | a 7-year-old male intact rottweiler was presented with a 1-week history of lethargy, anorexia, vomiting and multiple syncopal events. the results of the clinical examination and electrocardiography were consistent with a third degree atrioventricular block and an intermittent accelerated idioventricular rhythm. haematology, serum biochemistry, serology for borrelia burgdorferi, blood culture, total t4, thoracic radiography and echocardiography did not reveal the cause of the arrhythmia. response ... | 2007 | 18045349 |
| identification of host bloodmeal source and borrelia burgdorferi sensu lato in field-collected ixodes ricinus ticks in chaumont (switzerland). | to evaluate the importance of vertebrate species as tick hosts and as reservoir hosts in two endemic areas for lyme borreliosis in switzerland, we applied molecular methods for the analysis of bloodmeal source and borrelia infection in questing ixodes ricinus l. ticks. in total, 1326 questing ticks were simultaneously analyzed for borrelia and for blood meal remnants by using reverse line blot. an overall infection prevalence of 19.0% was recorded for borrelia sp., with similar rates in both sit ... | 2007 | 18047213 |
| optic neuropathy from viruses and spirochetes. | 2007 | 18049280 | |
| [borreliosis as the cause of disability pensions in norway]. | borreliosis is a bacterial infection transferred by tick-bites. neuroborreliosis is the most frequent disseminated form of the disorder in norway. registers exist in norway on all reported communicable diseases (the norwegian surveillance system for communicable diseases [msis]) and disability pension diagnoses (the norwegian directorate of labour and welfare). | 2007 | 18049495 |
| antibody responses to borrelia burgdorferi in patients with antibiotic-refractory, antibiotic-responsive, or non-antibiotic-treated lyme arthritis. | to compare the pattern of antibody responses to borrelia burgdorferi in patients with antibiotic-refractory, antibiotic-responsive, or non-antibiotic-treated lyme arthritis as an indirect measure of spirochetal persistence or eradication. | 2007 | 18050219 |
| detection of rickettsia amblyommii in association with a tick bite rash. | in the summer of 2006, an amblyomma americanum tick was removed from a woman in central north carolina, who subsequently developed a rash at the site of tick attachment. when examined by polymerase chain reaction (pcr) for borrelia, anaplasma, ehrlichia, babesia, rickettsia, and bartonella dna, only the rickettsia primers generated an amplicon, which was identified as "r. amblyommii" by sequencing. to our knowledge, this is the first case in which r. amblyommii was temporally associated with a r ... | 2007 | 18052716 |
| cd28 deficiency exacerbates joint inflammation upon borrelia burgdorferi infection, resulting in the development of chronic lyme arthritis. | lyme disease, caused by the tick-borne spirochete borrelia burgdorferi (bb), is a multisystem illness, affecting many organs, such as the heart, the nervous system, and the joints. months after bb infection, approximately 60% of patients experience intermittent arthritic attacks, a condition that in some individuals progresses to chronic joint inflammation. although mice develop acute arthritis in response to bb infection, the joint inflammation clears after 2 wk, despite continuous infection, o ... | 2007 | 18056348 |
| immune complexes in early lyme disease. | the study investigated the presence of borrelia-specific antibodies captured in immune complexes (ics) in patients with early lyme disease manifested by erythema migrans. out of 18 patients, 15 (83.3%) tested positive for polyethylene glycol-precipitated ics containing igm antibodies, while only 4 (22.2%) were igg positive. these results are in accordance with our findings obtained by standard elisa and recombinant blot, which indicated that ics might be used for serological diagnosis of the ear ... | 2007 | 18059570 |
| structural insights into the enzymatic mechanism of the pathogenic mapk phosphothreonine lyase. | the ospf family of phosphothreonine lyase, including spvc from salmonella, irreversibly inactivates the dual-phosphorylated host mapks (pt-x-py) through beta elimination. we determined crystal structures of spvc and its complex with a phosphopeptide substrate. spvc adopts a unique fold of alpha/beta type. the disordered n terminus harbors a canonical d motif for mapk substrate docking. the enzyme-substrate complex structure indicates that recognition of the phosphotyrosine followed by insertion ... | 2007 | 18060821 |
| phenotypic and genotypic analysis of borrelia spp. isolated from ixodes ricinus ticks by using electrophoretic chips and real-time polymerase chain reaction. | the genotype of borrelia burgdorferi sensu lato was detected in 371 out of 1244 ticks. borrelia determination was based on partial sequencing of the 16s rrna gene and real-time polymerase chain reactions for identification and quantitation of ospa and reca genes. different borrelia spp. were identified; b. garinii in 40% ticks followed by b. afzelii (36.3%), b. burgdorferi sensu stricto (12.9%), b. valaisiana (3.5%), b. lusitaniae (0.8%), b. bissettii (0.5%) and b. miyamotoi-like (0.5%). cultiva ... | 2007 | 18062179 |
| [what kind of clinical, epidemiological, and biological data is essential for the diagnosis of lyme borreliosis? dermatological and ophtalmological courses of lyme borreliosis]. | lyme borreliosis (bl) is a multisystem infectious tick-transmitted disease. the diversity of borrelia burgdorferi is the reason for a wide spectrum of dermatological and ophthalmologic presentations between patients from europe and from other countries. in europe, the main manifestations are dermatological. during the early stage, the diagnosis is clinical: finding erythema migrans (em) a few days after a tick bite is sufficient; several em mean an early-disseminated disease. borrelial lymphocyt ... | 2007 | 18065181 |
| spatial analysis of the exposure of dogs in rural north-coastal california to vectorborne pathogens. | between 0 and 50 per cent of the dogs in eight rural villages in far northern california with a high risk of tickborne diseases were seropositive for anaplasma phagocytophilum and bartonella vinsonii subspecies berkhoffii, and between 0 and 10 per cent were seropositive for borrelia burgdorferi. the odds ratio for the co-exposure of individual dogs to b vinsonii berkhoffii and a phagocytophilum was 18.2. none of the diseases was associated with the sex of the dogs, whether they slept out of door ... | 2007 | 17993656 |
| gencolors: annotation and comparative genomics of prokaryotes made easy. | gencolors (gencolors.fli-leibniz.de) is a new web-based software/database system aimed at an improved and accelerated annotation of prokaryotic genomes considering information on related genomes and making extensive use of genome comparison. it offers a seamless integration of data from ongoing sequencing projects and annotated genomic sequences obtained from genbank. a variety of export/import filters manages an effective data flow from sequence assembly and manipulation programs (e.g., gap4) t ... | 2007 | 17993668 |
| beta-strand flipping and slipping triggered by turn replacement reveal the opportunistic nature of beta-strand pairing. | we investigated how the register between adjacent beta-strands is specified using a series of mutants of the single-layer beta-sheet (slb) in borrelia ospa. the single-layer architecture of this system eliminates structural restraints imposed by a hydrophobic core, enabling us to address this question. a critical turn (turn 9/10) in the slb was replaced with a segment with an intentional structural mismatch. its crystal structure revealed a one-residue insertion into the central beta-strand (str ... | 2007 | 17985889 |
| retrobulbar optic neuritis: a complication of lyme disease? | 2007 | 18024698 | |
| opsoclonus myoclonus syndrome in two cases with neuroborreliosis. | 2007 | 18028183 | |
| [the modern therapy of lyme disease]. | the modern therapy of lyme disease, including newest guidelines of the infectious diseases society of america has been presented. poland is endemic region for borreliosis and number of reported cases have been increasing rapidly. newest guidelines should help doctors, not only infectious disease specialist, but especially general practicioners, solve problems assiciated with therapy of lyme disease. | 2007 | 18069380 |
| [significance of red deer (cervus elaphus) in the ecology of borrelia burgdorferi sensu lato]. | background: red deer (cervus elaphus) is one of the most important host of the adult tick (ixodes ricinus) which is the basic vector of the lyme disease causative agent--borrelia burgdorferi sensu lato in europe. the aim of the present study was to establish the role of red deer in the transmission of b. burgdorferi s.1. material and methods. tissues from 74 red deers were evaluated and the presence of b. burgdorferi s.1 dna was identified using nested pcr technique based on fla gene. the identi ... | 2007 | 18075156 |
| [frequency of antibodies anti-ehrlichia canis, borrelia burgdorferi and dirofilaria immitis antigens in dogs from microrregion ilhéus-itabuna, state of bahia, brazil]. | in order to detect the positivity to antigens of dirofilaria immitis, antibodies anti-borrelia burgdorferi and anti-ehrlichia canis, 200 canine blood samples were collected as followed: 100 from the municipality of ilhéus and 100 from itabuna, state of bahia. the kit snap 3dx (idexx laboratories) was used to performe serology. none of the tested animalswere positive for antigens of d. immitis. only two dogs of them were positive for antibodies anti-b. burgdorferi. from all the samples analyzed, ... | 2007 | 18078596 |
| evaluation of a genotyping method based on the ospa gene to detect borrelia burgdorferi sensu lato in multiple samples of lyme borreliosis patients. | in this study we have developed a new restriction-fragment-length-polymorphism (rflp) genotyping method for rapid detection and identification of borrelia genospecies present as unique species or as co-infection in multiple specimens obtained simultaneously from 29 individual patients affected by early or late lyme borreliosis (lb). the target of the rflp-genotyping was the heterogeneous plasmid located ospa gene, thus we developed a method able to detect and differentiate between six clinically ... | 2007 | 18080675 |
| [concentration of soluble forms of selectins in serum and in cerebrospinal fluid in group of patients with neuroborreliosis--a preliminary study]. | the results of the research already done, suggest an important role of selectins in inflammatory process of various etiology. lack of selectins or their ligands causes severe complications, such as chronic inflammatory processes. the aim of this study was to analyze the role of selectins sl, se and sp in the development and course of neuroborreliosis in the form of meningitis. we have also analyzed the influence of treatment on changes of selectins' concentration in serum and cerebrospinal fluid ... | 2007 | 18080689 |
| [heterogeneity of the gene p83/100 of borrelia borrelia burgdorferi sensu lato complex]. | the 35 full-length borrelia burgdorferi sensu lato complex a83/100 gene nucleotide sequences were determined. high level of homology was observed in the nucleotide sequences corresponding to the strains and isolates of borrelia fzelii. the analysis of the nucleotide sequences revealed two groups of borrelia garinii. the most variable p83/100 gene region containing species-typical insertions and deletions was demonstrated to be included into the region where the antigenic determinants of protein ... | 2007 | 18154079 |
| first molecular detection of borrelia afzelii in clinical samples in korea. | borrelia afzelii nucleic acids were detected in the sera of febrile disease patients by a nested pcr that targeted the rrf (5s)-rrl (23s) spacer of b. burgdorferi sensu lato. the b. afzelii-specific dna fragment was detected in 8 out of 283 sera which were proven to have immunoglobulin g or m antibodies against b. burgdorferi antigens through ifa. the results were further confirmed through restriction fragment length polymorphism and sequencing analysis of the dna fragments. the results indicate ... | 2007 | 18094538 |
| case report: borrelia valaisiana infection in a japanese man associated with traveling to foreign countries. | a 78-year-old japanese man who had clinical symptoms and a flu-like illness with fever, chills, diarrhea, and arthralgia had traveled to cambodia and khabarovsk, russia, before the onset of symptoms and illness. he had been bitten by an ixodes persulcatus tick in which the dna of borrelia valaisiana was detected. the patient's symptoms improved rapidly after treatment with minocycline. serologic examination detected antibodies to lyme disease borrelia. an flab polymerase chain reaction with the ... | 2007 | 18165534 |
| lyme disease presenting as ruptured synovial cysts. | 2007 | 18176154 | |
| anti-tumor necrosis factor-alpha activation of borrelia burgdorferi spirochetes in antibiotic-treated murine lyme borreliosis: an unproven conclusion. | 2007 | 18190269 | |
| anti-tumor necrosis factor-alpha treatment for lyme borreliosis. | 2007 | 18190271 | |
| ultrastructural evidence of the ehrlichial developmental cycle in naturally infected ixodes persulcatus ticks in the course of coinfection with rickettsia, borrelia, and a flavivirus. | ehrlichiae are small gram-negative obligately intracellular bacteria that multiply within vacuoles of their host cells and are associated for a part of their life cycle with ticks, which serve as vectors for vertebrate hosts. two morphologically and physiologically different ehrlichial cell types, reticulate cells (rc) and dense-cored cells (dc), are observed during experimental infection of cell cultures, mice, and ticks. dense-cored cells and reticulate cells in vertebrate cell lines alternate ... | 2007 | 18171109 |
| evaluation of a modified culture medium for borrelia burgdorferi sensu lato. | the aim of the present study was to assess the possible use of a modified medium, prepared in the laboratory using the constituents of barbour-stonner-kelly (bsk) medium and medium 199 as base, for the culture of borrelia strains, comparing the growth of individual strains in this medium and in the bsk-h medium, and the protein profile and antigenic characteristics of borrelia proteins expressed in these media. a qualitative evaluation of growth of borrelia species was made with acceptable resul ... | 2007 | 18209941 |
| zoonotic pathogens in ixodes scapularis, michigan. | 2007 | 18214207 | |
| concentration of tgf-beta1 in the supernatant of peripheral blood mononuclear cells cultures from patients with early disseminated and chronic lyme borreliosis. | the aberrant inflammatory response is probably involved in the pathogenesis of chronic lyme borreliosis, including chronic lyme arthritis and neuroborreliosis. transforming growth factor-beta 1 (tgf-beta1) is an important anti-inflammatory and immunomodulatory cytokine and its deficient synthesis is linked to exaggerated inflammation and immune response. | 2007 | 18217413 |
| lyme disease in urban areas, chicago. | 2007 | 18217578 | |
| antibodies to borrelia burgdorferi in erythema migrans patients. | determination of antibodies against borrelia burgdorferi has supporting value in the diagnose of lyme disease. the purpose of this study was to determine the production of antibodies in a defined group of patients. | 2007 | 18225477 |
| interannual dynamics of antibody levels in naturally infected long-lived colonial birds. | little is known about the long-term persistence of specific antibodies (ab) in natural host-parasite systems despite its potential epidemiological and ecological importance. in long-lived species, knowledge of the dynamics of individual immunological profiles can be important not only for interpreting serology results, but also for assessing transmission dynamics and the potential selective pressures acting on parasites. the aim of this paper was to investigate temporal variation in levels of sp ... | 2007 | 18229852 |
| [diagnostics of lyme disease]. | although many years have passed since borrelia burgdorferi was first identified, advances in understanding biology and clinical course of infection made and new diagnostic procedures developed, lyme disease is still difficult to diagnose. therefore, it is often wrongly diagnosed and unnecessarily treated. in this paper we analyzed the latest data on lyme disease diagnostic methods, paying much attention to their limitations and correct interpretation of results. in routine diagnosis of this dise ... | 2007 | 18274096 |
| [biochemical parameters of hepatobiliary system functions in viral hepatitis or ixodes tick-borne borreliosis concurrent with chronic opisthorchiasis]. | the biochemical parameters of hepatobiliary system functions were studied in patients with opisthorchiasis and concomitant diseases, such as chronic viral hepatitis concurrent with chronic opisthorchiasis, as well as ixodes tick-borne borreliosis in the presence of the same invasion. although the magnitude ofbiochemical changes is not great in chronic opisthorchiasis or chronic viral hepatitis, the concomitance of these two diseases were ascertained to result in pronounced abnormalities, by demo ... | 2007 | 18274146 |
| ticks and mosquitoes as vectors of borrelia burgdorferi s. l. in the forested areas of szczecin. | the aim of the study was to determine the infection level of adult forms and larvae of ticks and mosquitoes with borrelia burgdorferi in the forested areas of szczecin. a total of 1699 ticks ixodes ricinus, including 1422 nymphs, 277 adult forms and 2862 mosquito females representing the genera aedes (89.6%) and culex (10.4%) were collected between the years 2004 and 2005. a further 3746 larvae and 1596 pupae of culex pipiens pipiens were colleted from water bodies. borrelia burgdorferi s. l. wa ... | 2007 | 18274258 |
| biological aspects of lyme disease spirochetes: unique bacteria of the borrelia burgdorferi species group. | borrelia burgdorferi sensu lato is a group of at least twelve closely related species some of which are responsible for lyme disease, the most frequent zoonosis in europe and the usa. many of the biological features of borrelia are unique in prokaryotes and very interesting not only from the medical viewpoint but also from the view of molecular biology. | 2007 | 18345249 |
| functional analysis of a lipid galactosyltransferase synthesizing the major envelope lipid in the lyme disease spirochete borrelia burgdorferi. | one of the major lipids in the membranes of borrelia burgdorferi is monogalactosyl diacylglycerol (mgaldag), a glycolipid recently shown to carry antigenic potency. herein, it is shown that the gene mgs (tigr designation bb0454) of b. burgdorferi encodes for the protein bbmgs that, when expressed in escherichia coli, catalyzes the glycosylation of 1,2-diacylglycerol with specificity for the donor substrate udp-gal yielding mgaldag. related lipid enzymes were found in many gram-positive bacteria. ... | 2007 | 17456185 |
| microalbuminuria and comparison of serologic testing for exposure to borrelia burgdorferi in nonclinical labrador and golden retrievers. | canine lyme disease is caused by the spirochete borrelia burgdorferi after transmission by an ixodes tick, typically resulting in joint pain, fever and lethargy. lyme nephritis is a poorly characterized syndrome associated with severe glomerular and tubular renal injury and poor clinical outcome in young to middle-aged dogs positive for exposure to b. burgdorferi. the aims of this study were to identify associations between natural exposure to b. burgdorferi and the presence of microalbuminuria ... | 2007 | 17459861 |
| split target specificity of rest: a design for protein delivery, site selectivity and regulation of enzyme activity? | the rest telomere resolvase is responsible for maintaining the hairpin telomeres that cap the linear chromosome and minichromosomes of borrelia burgdorferi. this enzyme acts at the tandem telomere junctions present within circular dimers resulting from dna replication. rest mediates the transesterification steps of resolution using a constellation of active site residues similar to that found in tyrosine recombinases and type ib topoisomerases. by combining this reaction mechanism with a hairpin ... | 2007 | 17462008 |
| telomere resolution by borrelia burgdorferi rest through the collaborative efforts of tethered dna binding domains. | borrelia burgdorferi, a causative agent of lyme disease, has a highly unusual segmented genome composed of both circular molecules and linear dna replicons terminated by covalently closed hairpin ends or telomeres. replication intermediates of the linear molecules are processed into hairpin telomeres via the activity of rest, a telomere resolvase. we report here the results of limited proteolysis and mass spectroscopy to identify two main structural domains in rest, separated by a chymotrypsin c ... | 2007 | 17462009 |
| regulated synthesis of the borrelia burgdorferi inner-membrane lipoprotein ipla7 (p22, p22-a) during the lyme disease spirochaete's mammal-tick infectious cycle. | results of previous immunological studies suggested that borrelia burgdorferi regulates synthesis of the ipla7 lipoprotein during mammalian infection. through combined use of quantitative reverse transcription pcr, immunofluorescence analyses, elisa and immunoblotting, it is now demonstrated that ipla7 is actually expressed throughout mammalian infection, as well as during transmission both from feeding ticks to naïve mice and from infected mice to naïve, feeding ticks. however, proportions of i ... | 2007 | 17464050 |
| acute facial nerve palsy in children: how often is it lyme borreliosis? | acute facial nerve palsy in children may be caused by infection by borrelia burgdorferi, but the incidence of facial nerve palsy and the proportion of facial nerve palsy caused by lyme borreliosis may vary considerably between areas. furthermore, it is not well known how often facial nerve palsy caused by lyme borreliosis is associated with meningitis. in this population-based study, children admitted for acute facial nerve palsy to stavanger university hospital during 9 y from 1996 to 2004 were ... | 2007 | 17464865 |
| marginal zone b-cell depletion impairs murine host defense against borrelia burgdorferi infection. | marginal zone b (mzb) cells are a b-cell subset that produces t-cell-independent antibodies to blood-borne antigens. in this study, we examined the effects of mzb cell depletion on the immune response to the lyme disease spirochete borrelia burgdorferi, an extracellular pathogen for which t-cell-independent antibody is an important host defense. mzb cell depletion of c3h/hej mice using monoclonal antibody to lfa-1 and alpha(4)beta(1) integrins reduced b. burgdorferi-specific immunoglobulin m (ig ... | 2007 | 17470546 |
| identification of an il-17-producing nk1.1(neg) inkt cell population involved in airway neutrophilia. | invariant natural killer t (inkt) cells are an important source of both t helper type 1 (th1) and th2 cytokines, through which they can exert beneficial, as well as deleterious, effects in a variety of inflammatory diseases. this functional heterogeneity raises the question of how far phenotypically distinct subpopulations are responsible for such contrasting activities. in this study, we identify a particular set of inkt cells that lack the nk1.1 marker (nk1.1(neg)) and secrete high amounts of ... | 2007 | 17470641 |
| coinfection with borrelia turicatae serotype 2 prevents the severe vestibular dysfunction and earlier mortality caused by serotype 1. | relapsing fever (rf) is a multisystemic spirochetal infection caused by different borrelia species. studies in our laboratory have shown that disease severity varies depending on the infecting serotype. however, the relative contribution of each serotype to pathogenesis during mixed infections is not known. to investigate this, we compared the outcome of infection with isogenic serotypes 1 (bt1) or 2 (bt2) of the rf agent b. turicatae alone or in combination. | 2007 | 17471439 |
| determinants of the geographic distribution of puumala virus and lyme borreliosis infections in belgium. | vector-borne and zoonotic diseases generally display clear spatial patterns due to different space-dependent factors. land cover and land use influence disease transmission by controlling both the spatial distribution of vectors or hosts, and the probability of contact with susceptible human populations. the objective of this study was to combine environmental and socio-economic factors to explain the spatial distribution of two emerging human diseases in belgium, puumala virus (puuv) and lyme b ... | 2007 | 17474974 |
| tick-borne relapsing fever polymerase chain reaction of tick-borne relapsing fever caused by borrelia hermsii. | a patient presented to our hospital with symptoms consistent with relapsing fever. a blood specimen was positive by genus-specific polymerase chain reaction, and sequencing revealed borrelia hermsii. we propose the polymerase chain reaction as a possible alternative for currently used tests for the diagnosis of tick-borne relapsing fever. | 2007 | 17484230 |
| borrelia burgdorferi-induced monocyte chemoattractant protein-1 production in vivo and in vitro. | matrix metalloproteinase 9 (mmp-9) is selectively upregulated in erythema migrans (em) lesions with acute lyme disease. this study explored whether upregulation of mmp-9 was associated with monocyte chemoattractant protein 1 (mcp-1) production, and borrelia burgdorferi (b. burgdorferi) could induce mcp-1 production in vivo and in vitro. the results indicated that expression of mcp-1 was significantly increased in u937 cells by b. burgdorferi. the activity of mmp-9 could be elevated by recombinan ... | 2007 | 17485071 |
| [lyme disease]. | 2007 | 17491387 | |
| temperature-induced regulation of rpos by a small rna in borrelia burgdorferi. | the alternative sigma factor rpos (sigma38 or sigmas) plays a central role in the reciprocal regulation of the virulence-associated major outer surface proteins ospc and ospa in borrelia burgdorferi, the lyme disease spirochete. temperature is one of the key environmental signals controlling rpos, but the molecular mechanism by which the signal is transduced remains unknown. herein, we identify and describe a small non-coding rna, dsrabb, that regulates the temperature-induced increase in rpos. ... | 2007 | 17501929 |
| purine salvage pathways among borrelia species. | genome sequencing projects on two relapsing fever spirochetes, borrelia hermsii and borrelia turicatae, revealed differences in genes involved in purine metabolism and salvage compared to those in the lyme disease spirochete borrelia burgdorferi. the relapsing fever spirochetes contained six open reading frames that are absent from the b. burgdorferi genome. these genes included those for hypoxanthine-guanine phosphoribosyltransferase (hpt), adenylosuccinate synthase (pura), adenylosuccinate lya ... | 2007 | 17502392 |
| lyme disease update. | lyme disease is endemic to areas in both europe and the united states and the incidence is increasing. despite published guidelines, controversy persists about its diagnosis and management in patients who do not meet strict diagnostic criteria. this review summarizes important recently published studies and recommendations for the diagnosis and management of lyme disease. | 2007 | 17505186 |
| immunity against ixodes scapularis salivary proteins expressed within 24 hours of attachment thwarts tick feeding and impairs borrelia transmission. | in north america, the black-legged tick, ixodes scapularis, an obligate haematophagus arthropod, is a vector of several human pathogens including borrelia burgdorferi, the lyme disease agent. in this report, we show that the tick salivary gland transcriptome and proteome is dynamic and changes during the process of engorgement. we demonstrate, using a guinea pig model of i. scapularis feeding and b. burgdorferi transmission, that immunity directed against salivary proteins expressed in the first ... | 2007 | 17505544 |
| lyme neuroborreliosis: infection, immunity, and inflammation. | lyme neuroborreliosis (lnb), the neurological manifestation of systemic infection with the complex spirochaete borrelia burgdorferi, can pose a challenge for practising neurologists. this review is a summary of clinical presentation, diagnosis, and therapy, as well as of recent advances in our understanding of lnb. many new insights have been gained through work in experimental models of the disease. an appreciation of the genetic heterogeneity of the causative pathogen has helped clinicians in ... | 2007 | 17509489 |
| [role of biological assays in the diagnosis of lyme borreliosis presentations. what are the techniques and which are currently available?]. | the biological diagnosis of borrelia burgdorferi sensu lato infection is usually made by antibody detection in patient sera. thus, serological testing (elisa, immunoblotting) is essential for a biological diagnosis. specific antibody detection is usually done in serum and csf of patients suspected of lyme borreliosis. laboratories must follow european recommendations to validate these assays in routine practice. antibody detection lacks sensitivity in the early cutaneous phase of the infection. ... | 2007 | 17512148 |
| in vitro activities of faropenem, ertapenem, imipenem and meropenem against borrelia burgdorferi s.l. | little is known about the in vitro activity of penems and carbapenems against the spirochete borrelia burgdorferi. here, faropenem, ertapenem, imipenem and meropenem as well as the third-generation cephalosporin ceftriaxone and tobramycin were tested in vitro against 11 isolates of the b. burgdorferi sensu lato complex. on a microg/ml basis, ertapenem was the most potent carbapenem (minimal inhibitory concentration (mic) range: 0.015-0.125 microg/ml), with in vitro activity comparable with that ... | 2007 | 17512703 |
| dual binding specificity of a borrelia hermsii-associated complement regulator-acquiring surface protein for factor h and plasminogen discloses a putative virulence factor of relapsing fever spirochetes. | tick-borne relapsing fever in north america is primarily caused by the spirochete borrelia hermsii. the pathogen employs multiple strategies, including the acquisition of complement regulators and antigenic variation, to escape innate and humoral immunity. in this study we identified in b. hermsii a novel member of the complement regulator-acquiring surface protein (crasp) family, designated bhcrasp-1, that binds the complement regulators factor h (fh) and fh-related protein 1 (fhr-1) but not fh ... | 2007 | 17513779 |
| pathogen specificity and autoimmunity are distinct features of antigen-driven immune responses in neuroborreliosis. | neuroborreliosis (nb) is a chronic infectious disease of the central nervous system (cns) caused by a tick-borne spirochete, borrelia burgdorferi. in addition to direct effects of the causative infectious agent, additional immunity-mediated mechanisms are thought to play a role in the cns pathology of nb. in order to further understand the involvement of humoral immune mechanisms in nb, we dissected the intrathecal antibody responses down to the single-plasma-cell level. starting with single-cel ... | 2007 | 17517881 |
| prophylaxis after tick bites. | 2007 | 17521587 | |
| single-dose prophylaxis against lyme disease. | 2007 | 17521588 | |
| practice parameter: treatment of nervous system lyme disease (an evidence-based review): report of the quality standards subcommittee of the american academy of neurology. | to provide evidence-based recommendations on the treatment of nervous system lyme disease and post-lyme syndrome. three questions were addressed: 1) which antimicrobial agents are effective? 2) are different regimens preferred for different manifestations of nervous system lyme disease? 3) what duration of therapy is needed? | 2007 | 17522387 |
| spirochetemia caused by borrelia turicatae infection in 3 dogs in texas. | spirochetemia was diagnosed in 2 siberian huskies and a rottweiler from the northwestern region of texas between june 1999 and october 2001. clinical findings were nonspecific; tick exposure was documented in 2 of the dogs. hematologic abnormalities included anemia (n=2), neutrophilia (n=2, including 1 with a left shift), lymphopenia (n=3), eosinopenia (n=3), and thrombocytopenia (n=2). one anemic dog had a positive coombs' test. in 1 dog, western blot analysis of serum yielded multiple positive ... | 2007 | 17523100 |
| erythema migrans: a reassessment of diagnostic criteria for early cutaneous manifestations of borreliosis with particular emphasis on clonality investigations. | controversy exists about the relationship of borrelia infection with b-cell lymphomas because b-cell clonality has been identified in infiltrates that contained borrelia-specific dna. systematic clinicopathological, immunophenotypical and molecular pathological studies of early borreliosis are lacking. | 2007 | 17535225 |
| epitope length, genospecies dependency, and serum panel effect in the ir6 enzyme-linked immunosorbent assay for detection of antibodies to borrelia burgdorferi. | in the absence of erythema migrans, the basis for diagnosis of lyme disease is the demonstration of an antibody response against borrelia burgdorferi in an appropriate clinical setting. the c6 enzyme-linked immunosorbent assay, based on the ir6 region of vlse, has become widely used in both the united states and europe. we mapped the antigenic epitopes of ir6 to a shorter sequence that is equivalent in sensitivity and specificity to the full-length ir6 25-residue peptide. in addition, we observe ... | 2007 | 17538122 |
| binding of human factor h-related protein 1 to serum-resistant borrelia burgdorferi is mediated by borrelial complement regulator-acquiring surface proteins. | isolates of borrelia burgdorferi, the causative agent of lyme disease, express up to 5 distinct complement regulator-acquiring surface proteins (crasp-1, -2, -3, -4, and -5). | 2007 | 17538892 |
| louse-borne relapsing fever and malaria co-infection in ethiopia. | 2007 | 17540107 | |
| the critical role of the linear plasmid lp36 in the infectious cycle of borrelia burgdorferi. | borrelia burgdorferi, the aetiological agent of lyme disease, follows a life cycle that involves passage between the tick vector and the mammalian host. to investigate the role of the 36 kb linear plasmid, lp36 (also designated the b. burgdorferi k plasmid), in the infectious cycle of b. burgdorferi, we examined a clone lacking this plasmid, but containing all other plasmids known to be required for infectivity. our results indicated that lp36 was not required for spirochete survival in the tick ... | 2007 | 17542926 |
| transmission of bacterial agents from lone star ticks to white-tailed deer. | amblyomma americanum (l.), the lone star tick, is an aggressive ixodid tick that has been implicated as a vector for several bacteria. among these bacteria are the disease agents ehrlichia chaffeensis and ehrlichia ewingii, and the putative disease agent "borrelia lonestari." the hypothesis in this study was that wild lone star ticks from northeastern georgia are capable of transmitting all three agents to white-tailed deer, odocoileus virginianus, a known reservoir host for e. chaffeensis. in t ... | 2007 | 17547234 |
| tissue and life-stage distribution of a defensin gene in the lone star tick, amblyomma americanum. | the transcript sequence of the amblyomma americanum linnaeus (acari: ixodidae) defensin, termed amercin (amn), was ascertained and a 219-bp amn coding region identified. the gene encodes a 72-amino acid prepropeptide with a putative 37-amino acid mature peptide. this gene shows little similarity to either of the defensins from amblyomma hebraeum koch, the only other amblyomma species for which a defensin has been described. sequence comparisons with other tick defensins reveal amn to be shorter ... | 2007 | 17550433 |
| diversity and distribution of borrelia hermsii. | borrelia hermsii is the most common cause of tickborne relapsing fever in north america. dna sequences of the 16s-23s rdna noncoding intergenic spacer (igs) region were determined for 37 isolates of this spirochete. these sequences distinguished the 2 genomic groups of b. hermsii identified previously with other loci. multiple igs genotypes were identified among isolates from an island, which suggested that birds might play a role in dispersing these spirochetes in nature. in support of this the ... | 2007 | 17552097 |