Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| dna fingerprinting analysis of breakthrough outbreaks in vaccine-protected poultry stocks. | we report recurrent outbreaks of yersinia pseudotuberculosis conjunctivitis in ducks and of fowl cholera in geese, occurring in stocks previously vaccinated with inactivated autogenous vaccines. enterobacterial repetitive intergenic consensus sequence-based pcr and pulsed-field gel electrophoresis indicated reinfection with a new y. pseudotuberculosis strain and vaccine evasion by the same pasteurella multocida strain. | 2007 | 17928428 |
| susceptibility of bacterial pathogens against lincomycin/spectinomycin (1/2), penicillin g/neomycin (1/1), and penicillin g/dihydrostreptomycin (1/1) as determined in the bft-germvet monitoring program 2004-2006. | a total of 368 bacterial pathogens, including 72 coagulase-positive and coagulase-variable staphylococci, 97 beta-haemolytic streptococci, 51 escherichia coli, 75 pasteurella multocida, 25 mannheimia haemolytica, 25 pseudomonas aeruginosa, and 23 arcanobacterium pyogenes, were investigated for their susceptibility to the three combinations of antimicrobial agents lincomycin/spectinomycin (1/2), penicillin g/neomycin (1/1), and penicillin g/dihydrostreptomycin (1/1) in comparison to their suscept ... | 2007 | 17939451 |
| antimicrobial susceptibility of pasteurella multocida and bordetella bronchiseptica from dogs and cats as determined in the bft-germvet monitoring program 2004-2006. | a total of 92 canine/feline pasteurella multocida strains form respiratory tract infections or infections of skin/ear/mouth as well as 42 canine/feline bordetella bronchiseptica strains from respiratory tract infections were investigated for their susceptibility to antimicrobial agents. while the p. multocida strains were susceptible to all antimicrobial agents tested - except sulfonamides -, a considerable number of the b. bronchiseptica strains was resistant or exhibited high mic values agains ... | 2007 | 17939457 |
| quantitative resistance level (mic) of pasteurella multocida isolated from pigs between 2004 and 2006: national resistance monitoring by the bvl. | the national resistance monitoring of the federal office of consumer protection and food safety (bvl) is to determine the prevalence of resistance of bacterial pathogens from animals using a valid database. from 2004 to 2006, a total of 1,472 pasteurella multocida strains isolated from pigs with acute respiratory tract diseases was submitted to the bvl and examined. of these, 1,11 (75.5 %) were included in the study and tested using 24 different antimicrobial substances. the results showed that ... | 2007 | 17939459 |
| quantitative resistance level (mic) of bacterial pathogens (escherichia coli, pasteurella multocida, pseudomonas aeruginosa, salmonella sp., staphylococcus aureus) isolated from chickens and turkeys: national resistance monitoring by the bvl 2004/2005. | in the study 2004/2005, the current quantitative resistance level of escherichia coli, pasteurella multocida, pseudomonas aeruginosa, salmonella sp. and staphylococcus aureus from chickens and turkeys was determined for the first time within the framework of the national resistance monitoring of the federal office of consumer protection and food safety (bvl). the objective was to implement a valid database on the basis of which the development and spread of resistance can be evaluated and monito ... | 2007 | 17939460 |
| reversible myocardial depression associated with sepsis in a dog. | 2007 | 17939574 | |
| accuracy of susceptibility testing of pasteurella multocida and mannheimia haemolytica. | the accuracy of antimicrobial susceptibility determinations relies on the bacterial species identification and the test methodology that is being used. in the present study, both aspects were investigated for 141 pasteurella multocida and 34 mannheimia haemolytica sensu lato isolates recovered from the upper respiratory tract of healthy calves. this was performed on the one hand by comparing of classical phenotyping with tdna-pcr genotyping and on the other hand by pairwise comparison of disk di ... | 2007 | 17949308 |
| pasteurella multocida bacteremia in asymptomatic plateletpheresis donors: a tale of two cats. | bacterial contamination of platelet (plt) concentrates occurs in 1 in 1000 to 1 in 3000 components and has been a leading cause of transfusion-associated morbidity and mortality. two cases of pasteurella multocida bacteremia in asymptomatic plateletpheresis donors are reported. clinical outcomes were profoundly different, emphasizing the importance of robust methods to detect bacterial contamination. | 2007 | 17958526 |
| shuffling of pig interleukin-2 gene and its enhancing of immunity in mice to pasteurella multocida vaccine. | in order to explore the safe and effective adjuvant for promotion of immunity of animals against infection, the experiment was carried out to shuffle tibet pig il-2 cdna with other il-2 cdna from human, yak and mouse, and the effect of shuffled il-2 (il-2s) gene in vivo was investigated on immunity of mice to pasteurella multocida. the il-2s protein was found to remarkably promote the proliferation of pig lymphoblasts than the native pig il-2 protein. then the il-2s gene was cloned into vr1020 e ... | 2007 | 17959278 |
| comparison of the in vitro susceptibility of rodent isolates of pseudomonas aeruginosa and pasteurella pneumotropica to enrofloxacin. | the objectives of this study were to determine and compare the in vitro enrofloxacin susceptibility of 94 pseudomonas aeruginosa isolates obtained from enrofloxacin-treated and untreated mice and that of 40 pasteurella pneumotropica strains and also to assess the efficacy and effects of enrofloxacin treatment of laboratory mice. the minimum inhibitory concentrations (mics) of enrofloxacin against all the ps. aeruginosa isolates were in the range of 1 to 4 microg/ml, whereas those against all the ... | 2007 | 17823403 |
| sinonasal plasmacytoma in a cat. | a 13-year-old female spayed domestic shorthair cat presented with a history of right-sided mucopurulent nasal discharge for 18 months. computed tomography revealed a mass within the right nasal cavity and the right frontal sinus. the animal was euthanized, and a postmortem examination was performed. on macroscopic examination, the right nasal cavity and the right frontal sinus were partially occluded by a soft whitish mass. microscopically, the mass was composed of well-differentiated plasma cel ... | 2007 | 17823407 |
| immunological roles of pasteurella multocida toxin (pmt) using a pmt mutant strain. | the immunological role of the pasteurella multocida toxin (pmt) in mice was examined using a pmt mutant strain. after a nasal inoculation, the mutant strain failed to induce interstitial pneumonia. moreover, pmt had no significant effect on the populations of cd4+, cd8+, cd3+, and cd19+ immunocytes in blood or on the populations of cd4+ and cd8+ splenocytes (p<0.01). however, there was a significant increase in the total number of cells in the bal samples obtained from the wild-type p. multocida ... | 2007 | 17846592 |
| evaluation of goose serum amyloid a acute phase response by enzyme-linked immunosorbent assay. | serum amyloid a (saa) is of interest as the circulating precursor of amyloid a protein, the fibrillar component of aa (secondary) amyloid deposits, and also as an extremely sensitive and rapid major acute phase protein. serum concentrations of acute phase proteins (apps) provide valuable information about the diagnosis and prognosis of various diseases, and thus the relevance of apps for monitoring the health status of domestic animals is widely accepted. more importantly, the measurement of saa ... | 2007 | 17867462 |
| safety and protective efficacy of intramuscular vaccination with a live aroa derivative of pasteurella multocida b:2 against experimental hemorrhagic septicemia in calves. | three groups of five calves, namely, v1, v2, and v3, were immunized intramuscularly at 4 and 8 weeks of age with ca. 10(9), 10(8), and 10(7) cfu, respectively, of a derivative of pasteurella multocida b:2 wild-type strain 85020 containing a deletion in the aroa gene (strain jrmt12). the first and second vaccinations resulted in significantly (p < 0.01) higher rectal temperature responses in groups v1 and v2 than in group v3. serum immunoglobulin m (igm) and igg titers did not increase in any gro ... | 2007 | 17875632 |
| a multidomain outer membrane protein from pasteurella multocida: modelling and simulation studies of pmompa. | pmompa is a two-domain outer membrane protein from pasteurella multocida. the n-terminal domain of pmompa is a homologue of the transmembrane beta-barrel domain of ompa from escherichia coli, whilst the c-terminal domain of pmompa is a homologue of the extra-membrane neisseria meningitidis rmpm c-terminal domain. this enables a model of a complete two domain pmompa to be constructed and its conformational dynamics explored via md simulations of the protein embedded within two different phospholi ... | 2007 | 17888868 |
| action of pasteurella multocida toxin on galpha(q) is persistent and independent of interaction with g-protein-coupled receptors. | pasteurella multocida toxin (pmt) activates galpha(q) and facilitates stimulation of inositol phosphate accumulation induced by agonists via g(q)-coupled membrane receptors. here, we studied the effects of pmt on agonist-induced gtpgammas binding to g(q) in cell membranes and a role of g-protein-coupled receptors in the action of pmt. pre-treatment of swiss 3t3 cells with pmt increased bombesin or vasopressin-induced gtpgammas-binding in cell membranes by about 50 to 150%. increase in agonist-st ... | 2007 | 17669624 |
| purification, crystallization and preliminary crystallographic characterization of the alpha 2,6-sialyltransferase from photobacterium sp. jt-ish-224. | sialyltransferases transfer sialic acid from cytidine-5-monophospho-n-acetylneuraminic acid (cmp-neuac) to the nonreducing termini of the oligosaccharyl structures of various glycoproteins and glycolipids. the newly cloned alpha2,6-sialyltransferase from photobacterium sp. jt-ish-224 (from the vibrionaceae family) is composed of two domains: an unknown n-terminal domain and a catalytic c-terminal domain which shares significant homology with the pasteurella multocida multifunctional sialyltransf ... | 2007 | 17671362 |
| variation in the molecular weight of photobacterium damselae subsp. piscicida antigens when cultured under different conditions in vitro. | the antigenicity of photobacterium damselae (ph. d.) subsp. piscicida, cultured in four different growth media [tryptone soya broth (tsb), glucose-rich medium (grm), iron-depleted tsb (tsb + ir(-)), and iron-depleted grm (grm + ir(-))] was compared by enzyme-linked immunosorbent assay (elisa) and western blot analysis using sera obtained from sea bass (dicentrarchus labrax) raised against live or heat-killed ph. d. subsp. piscicida. the antigenic expression of ph. d. subsp. piscicida was found t ... | 2007 | 17679772 |
| [severe pasteurella multocida pneumonia by close contact with a pet in chronic respiratory failure]. | 2007 | 17682434 | |
| pasteurella multocida septic shock following liver transplantation treated with drotrecogin alpha (activated). | severe sepsis and progression to septic shock in solid organ transplant recipients is associated with a high mortality. we describe a fulminant case of septic shock in a liver transplant recipient caused by pasteurella multocida, a gram-negative coccobacillus most commonly associated with domestic cats and dogs. p. multocida is a rare cause of bacteremia and has not been reported as a cause of septic shock following liver transplantation. in addition to standard therapy, the patient was managed ... | 2007 | 17692071 |
| [knee prosthesis infection due to pasteurella multocida]. | 2007 | 17692220 | |
| peritonitis associated with pasteurella multocida in peritoneal dialysis patients--case report and review of the literature. | pasteurella multocida is a zoonotic pathogen found in the oral cavities of domestic dogs and cats and other wild and domestic animals. 14 cases ofperitoneal dialysis-associated pasteurella multocida peritonitis linked to animal contact have been reported in the literature to date. in each case, the source of infection was believed to be a domestic cat or cats. one case of spontaneous bacterial peritonitis has been reported in a cirrhotic patient and was not linked to animal contact. poor hygiene ... | 2007 | 17703837 |
| nucleotide sequence and transfer properties of two novel types of actinobacillus pleuropneumoniae plasmids carrying the tetracycline resistance gene tet(h). | to analyse the sequence and transfer properties of two tetracycline resistance plasmids found in clinical isolates of actinobacillus pleuropneumoniae in order to assess their role in the spread of tetracycline resistance. | 2007 | 17704116 |
| decoration of pasteurella multocida lipopolysaccharide with phosphocholine is important for virulence. | phosphocholine (pcho) is an important substituent of surface structures expressed by a number of bacterial pathogens. its role in virulence has been investigated in several species, in which it has been shown to play a role in bacterial adhesion to mucosal surfaces, in resistance to antimicrobial peptides, or in sensitivity to complement-mediated killing. the lipopolysaccharide (lps) structure of pasteurella multocida strain pm70, whose genome sequence is known, has recently been determined and ... | 2007 | 17704225 |
| recombinant synthesis of hyaluronan by agrobacterium sp. | hyaluronan (ha) is a sugar polymer of a repeating disaccharide, beta1-3 d-n-acetylglucosamine (glcnac) beta1-4 d-glucuronic acid (glca). it finds applications in numerous biomedical procedures such as ophthalmic surgery and osteoarthritis treatment. until recently, the only commercial sources were extraction of rooster combs and from fermentation of pathogenic streptococcus. in this work, we demonstrate that metabolic engineering strategies enable the recombinant synthesis of hyaluronan in a saf ... | 2007 | 17705506 |
| contamination of post-endoscopic sinus surgery sinus cavities with pasteurella multocida. | 2007 | 17711761 | |
| pasteurella multocida sepsis and meningitis in 2-month-old twin infants after household exposure to a slaughtered sheep. | this article outlines 2 cases of pasteurella multocida sepsis and meningitis in 2-month-old twins after their father was exposed to a sheep during a sacrifice celebration at home. these cases emphasize the necessity of respecting basic hygiene rules and the danger involved in animal sacrifice without suitable professional structure. | 2007 | 17712748 |
| calcineurin-independent inhibition of 3t3-l1 adipogenesis by pasteurella multocida toxin: suppression of notch1, stabilization of beta-catenin and pre-adipocyte factor 1. | pasteurella multocida toxin (pmt) is a potent mitogen and a specific activator of gq-dependent signalling pathways. pmt impairs osteoblast differentiation and causes bone loss and fat reduction in vivo. we examined the effect of pmt on cell signalling pathways involved in 3t3-l1 adipocyte differentiation. we demonstrate that pmt treatment before or together with differentiation induction factors inhibits adipogenesis and prevents upregulation of important adipocyte markers - peroxisome-prolifera ... | 2007 | 17581254 |
| detection of pathogenic leptospires in animals by pcr based on lipl21 and lipl32 genes. | efficacy of primers capable of amplifying conserved outer membrane protein (omp) genes i.e., lipl21 and lipl32 of leptospira strains was tested for rapid and early diagnosis of the leptospirosis using a polymerase chain reaction (pcr). these omp genes were found to be conserved in various leptospiral serovars viz., canicola, pomona, icterohaemorrhagiae, pyrogenes, sejroe, grippotyphosa, ballum and tarassovi as pcr products of 561 bp and 756 bp were obtained by pcr employing lipl21 and lipl32 bas ... | 2007 | 17585694 |
| staphylococcal infections in rabbit does on two industrial farms. | the main reasons for culling adult rabbit does on two spanish rabbit farms were investigated for a year. the most important conditions were mastitis (33.3 per cent), followed by subcutaneous abscesses (9.9 per cent) and pyometra (8.7 per cent). staphylococcus aureus infections were the most severe problem, the organism being isolated from 69.2 per cent of infected animals. pasteurella species were more prevalent in cases of pyometra and pneumonia. two strains of s aureus were identified by using ... | 2007 | 17586791 |
| immune response in mice and swine to dna vaccines derived from the pasteurella multocida toxin gene. | dna vaccines were constructed with either a 5'-truncated or full-length, genetically detoxified toxin gene from pasteurella multocida and two different dna vaccine vectors, distinguished by the presence or absence of a secretion signal sequence. optimal pmt-specific antibody responses and spleen cell secretion of interferon-gamma following immunization of mice were achieved with pmm4, the construct containing a signal sequence and encoding the entire toxin. antibody responses were also induced i ... | 2007 | 17590484 |
| [monitoring of infectious diseases among wild boars]. | sixty-seven serum samples and 43 pathological material samples from wild boars, taken in 5 regions of russia and in the kharkov region of the ukraine in 2002 to 2005 were studied. wild boars in some regions of russia were shown to be carriers of aujeszky's disease virus, porcine parvovirus, porcine circovirus type 2, lymphotropic herpesvirus-1, porcine cytomegalovirus, mycoplasma hyopneumoniae and pasteurella multocida. the classical swine fever (csf) virus genome was detected by polymerase chai ... | 2007 | 17601049 |
| evaluation of an indirect enzyme-linked immunosorbent assay (elisa) using recombinant toxin for detection of antibodies against pasteurella multocida toxin. | to facilitate the control of progressive atrophic rhinitis (par) of swine caused by toxigenic pasteurella multocida, an enzyme-linked immunosorbent assay (elisa) and a serum neutralization test (nt) have recently been developed to detect antibodies against the p. multocida dermonecrotic toxin (pmdnt). however, the nt is a cumbersome and time-consuming technique. to overcome these drawbacks, we developed an indirect elisa, using recombinant pmdnt expressed in escherichia coli, for the detection o ... | 2007 | 17611352 |
| nucleoside analogues are activated by bacterial deoxyribonucleoside kinases in a species-specific manner. | to investigate the bactericidal activity of antiviral and anticancer nucleoside analogues against a variety of pathogenic bacteria and characterize the activating enzymes, deoxyribonucleoside kinases (dnks). | 2007 | 17615154 |
| clinical and in vitro efficacy of amoxicillin against bacteria associated with feline skin wounds and abscesses. | a clinical trial involving 122 cats with infected skin wounds or abscesses presented to 10 veterinary clinics was conducted to evaluate the efficacy of 2 oral amoxicillin drug products (a paste and a suspension). a 2nd objective of the study was to identify bacteria involved in such infections and verify their in vitro sensitivity to amoxicillin. samples of wound exudate were harvested at the time of presentation and submitted for aerobic and anaerobic culture. the sensitivity to amoxicillin of ... | 2007 | 17616057 |
| expression of the dermonecrotic toxin by bordetella bronchiseptica is not necessary for predisposing to infection with toxigenic pasteurella multocida. | this experiment was designed to determine whether a bordetella bronchiseptica mutant that does not produce dermonecrotic toxin (dnt) is still capable of predisposing pigs to infection with toxigenic pasteurella multocida. three groups of pigs were initially inoculated intranasally with a wild type b. bronchiseptica that produces dnt, an isogenic mutant of b. bronchiseptica that does not produce dnt, or pbs. all pigs were then challenged intranasally with a toxigenic strain of p. multocida 4 days ... | 2007 | 17624695 |
| chemoenzymatic synthesis with distinct pasteurella heparosan synthases: monodisperse polymers and unnatural structures. | heparosan (-glcua-beta1,4-glcnac-alpha1,4-)(n) is a member of the glycosaminoglycan polysaccharide family found in the capsule of certain pathogenic bacteria as well as the precursor for the vertebrate polymers, heparin and heparan sulfate. the two heparosan synthases from the gram-negative bacteria pasteurella multocida, pmhs1 and pmhs2, were efficiently expressed and purified using maltose-binding protein fusion constructs. these relatively homologous synthases displayed distinct catalytic cha ... | 2007 | 17627940 |
| histidine residues at the active site of the pasteurella multocida toxin. | we have investigated histidine residues near the active site of the mitogenic pasteurella multocida toxin. mutation of h1202 or h1228 had little effect, while the effect of mutation on h1223 depended on the amino acid substituted. mutation of h1205 caused complete loss of activity, indicating its importance in pmt activity. | 2007 | 18949067 |
| pathogenic and opportunistic gram-negative bacteria in soil, leachate and air in san nicolás landfill at aguascalientes, mexico. | the occurrence of gram-negative pathogenic and opportunistic species, was studied for two years on air. soil and leachate from the san nicolás landfill. aguascalientes, mexico. for soil and leachate four samplings were done, two during the dry season and two during the rainy season. for soil there were 15 sampling points, the leachate samples were taken on the leachate tank. for air, twelve sampling were done in three points of the landfill. twenty pathogenic and/or opportunistic bacteria were i ... | 2007 | 18693549 |
| effect of centchroman on cellular and humoral immunity. | centchroman (ormeloxifene) is a nonsteroidal selective estrogen receptor modulator that is used as once a week oral contraceptive agent. the effect of centchroman on the immune system was evaluated by using different experimental models such as carbon clearance test, cyclophosphamide induced neutropenia, neutrophil adhesion test, effect on serum immunoglobulins, mice lethality test and indirect haemagglutination test. the first three models namely carbon clearance test, cyclophosphamide induced ... | 2007 | 18476393 |
| occurrence of antimicrobial resistance in bacteria from diagnostic samples from dogs. | to study the occurrence of antimicrobial resistance among common bacterial pathogens from dogs and relate resistance patterns to data on consumption of antimicrobials. | 2007 | 17644533 |
| [pasteurella multocida pneumonia with molecular evidence of zoonotic transmission]. | we report the case of a patient with pneumonia caused by pasteurella multocida (p. multocida). an 83-year-old woman admitted for bronchiectasis, productive cough, and a high fever was found in chest radiography on admission to have a new infiltrative shadow in the left lower lung field. chest computed tomography indicated bilateral bronchiectasis and an infiltrative lingular shadow. p. multocida was isolated from sputum samples. biapenem and minocycline therapy ameliorated symptoms. pulsed-field ... | 2007 | 18095473 |
| phenotypic and genotypic characterisation of pasteurella multocida strains isolated from pigs in hungary. | a total of 146 pasteurella multocida strains isolated from swine in hungary in the last 20 years were examined. biochemical characterisation and pcr-based techniques were used to determine species, subspecies, biovar, capsule type and presence of the toxa gene. eighty-seven percent of the isolates belonged to p. multocida ssp. multocida, and 98% of these had biovar 3 or were trehalose- or lactose-fermenting or ornithine decarboxylase negative variants of that. ten percent of the strains were p. ... | 2007 | 18277702 |
| pasteurella multocida infections. | 2007 | 18055649 | |
| [frequency of occurrence of pneumonia associated agents and their correlation with clinical and pathological-anatomical findings in pigs]. | the aim of this study was to investigate the incidence and influence of different causative organisms involved in the development of pneumonia and bronchopneumonia in pigs. bronchoalveolar lavage fluid (balf) from 339 pigs was examined molecular-biologically and culturally. the evaluation considered the following pathogens: mycoplasma (m.) hyopneumoniae, mycoplasma (m.) hyorhinis, prrsv (us-type), prrsv (eu-type), pcv-2, influenzavirus type a, alpha-haemolytic streptococci, beta-haemolytic strep ... | 2007 | 18085159 |
| cardiovascular device infections in dogs: report of 8 cases and review of the literature. | infection is an infrequent but major complication of cardiovascular device implantation. | 2007 | 18196736 |
| pasteurella multocida and bovine respiratory disease. | pasteurella multocida is a pathogenic gram-negative bacterium that has been classified into three subspecies, five capsular serogroups and 16 serotypes. p. multocida serogroup a isolates are bovine nasopharyngeal commensals, bovine pathogens and common isolates from bovine respiratory disease (brd), both enzootic calf pneumonia of young dairy calves and shipping fever of weaned, stressed beef cattle. p. multocida a:3 is the most common serotype isolated from brd, and these isolates have limited ... | 2007 | 18218157 |
| immune evasion by pathogens of bovine respiratory disease complex. | bovine respiratory tract disease is a multi-factorial disease complex involving several viruses and bacteria. viruses that play prominent roles in causing the bovine respiratory disease complex include bovine herpesvirus-1, bovine respiratory syncytial virus, bovine viral diarrhea virus and parinfluenza-3 virus. bacteria that play prominent roles in this disease complex are mannheimia haemolytica and mycoplasma bovis. other bacteria that infect the bovine respiratory tract of cattle are histophi ... | 2007 | 18218162 |
| microorganisms associated with a pneumonic epizootic in rocky mountain bighorn sheep (ovis canadensis canadensis). | a comprehensive study of a pneumonic epizootic was initiated when the first signs of disease were noted in a metapopulation of bighorn sheep inhabiting hells canyon, bordering idaho, oregon, and washington. a total of 92 bighorn sheep were tested for etiologic agents during the following 6-mo study period. the study population included bighorn sheep believed to be the subpopulation in which disease was first noted, and these sheep were translocated to a holding facility in an effort to contain t ... | 2007 | 18229860 |
| [acclimating, screening of dominant bacteria for degrading phthalic acid esters and study on its degradation]. | to define the method of acclimating isolating and screening of dominant bacteria for phthalic acid esters biodegradation and study their plasmids. | 2007 | 18303624 |
| synthesis and biological study of medicinally important mannich bases derived from 4-(dimethylamino)-1,4,4a,5,5a,6,11,12a-octahydro-3,6,10,12,12a pentahydroxy naphthacene carboxamide. | the paper describes synthesis and antibacterial study of biologically active mannich bases of carboxamide derivative employing mannich reaction of 4-(dimethylamino)-1,4,4a,5,5a,6,11,12a-octahydro-3,6,10,12,12a pentahydroxy naphthacene carboxamide with various sulfonamides/secondary amines . they were analysed by elemental analysis and characterized by uv, ir and (1)h nmr spectroscopic studies. the mannich bases were screened for antibacterial activity against various gram-negative bacteria at va ... | 2007 | 17110110 |
| the functional molecular mass of the pasteurella hyaluronan synthase is a monomer. | hyaluronan (ha), a linear polysaccharide composed of beta1,3-glcnac-beta1,4-glcua repeats, is found in the extracellular matrix of vertebrate tissues as well as the capsule of several pathogenic bacteria. all known ha synthases (hass) are dual-action glycosyltransferases that catalyze the addition of two different sugars from udp-linked precursors to the growing ha chain. the bacterial hyaluronan synthase, pmhas from gram-negative pasteurella multocida, is a 972-residue membrane-associated prote ... | 2007 | 17095162 |
| full genome sequence and some biological properties of reticuloendotheliosis virus strain apc-566 isolated from endangered attwater's prairie chickens. | reticuloendotheliosis virus (rev) causes runting, high mortality, immunosuppression, and chronic neoplasia associated with t and/or b cell lymphomas in a variety of domestic and wild birds, including attwater's prairie chickens (apc) (tympanuchus cupido attwateri). the complete proviral sequence of a recent rev isolate from apc (rev apc-566) was determined. this virus was isolated from an apc maintained in captivity in a reproduction program intended to avoid its extinction. rev apc-566 was dete ... | 2007 | 17098316 |
| acceptor specificity of the pasteurella hyaluronan and chondroitin synthases and production of chimeric glycosaminoglycans. | the hyaluronan (ha) synthase, pmhas, and the chondroitin synthase, pmcs, from the gram-negative bacterium pasteurella multocida polymerize the glycosaminoglycan (gag) sugar chains ha or chondroitin, respectively. the recombinant escherichia coli-derived enzymes were shown previously to elongate exogenously supplied oligosaccharides of their cognate gag (e.g. ha elongated by pmhas). here we show that oligosaccharides and polysaccharides of certain noncognate gags (including sulfated and iduronic ... | 2007 | 17099217 |
| etiology of respiratory disease in non-vaccinated, non-medicated calves in rearing herds. | the aim of this study was to examine the occurrence of bacterial, mycoplasmal and viral pathogens in the lower respiratory tract of calves in all-in all-out calf-rearing units. according to clinical status, non-medicated calves with and without respiratory disease signs were selected of the 40 herds investigated to analyse the micro-organisms present in healthy and diseased calves. tracheobronchial lavage (tbl) and paired serum samples were analysed for bacteria, mycoplasmas, respiratory syncyti ... | 2007 | 17084565 |
| identification of novel immunogens in pasteurella multocida. | p. multocida is a gram-negative pathogen responsible for causing diseases in animals of economic significance to livestock industries throughout the world. current vaccines include bacterins, which provide only limited protection against homologous serotypes. therefore there is a need for more effective vaccines to control diseases caused by p. multocida. as a step towards developing vaccines against fowl cholera, a genomics based approach was applied for the identification of novel immunogens. ... | 2007 | 17233917 |
| the response of mannheimia haemolytica to iron limitation: implications for the acquisition of iron in the bovine lung. | mannheimia haemolytica is the major causative agent of shipping fever, a severe pneumonia in cattle causing high morbidity and mortality. a prerequisite of successful lung colonization by m. haemolytica is the necessity to adapt to the paucity of iron. the lack of genome information has precluded an assessment of the genetic repertoire available to m. haemolytica to adapt to low iron environments. to close this knowledge-gap, we have determined 90% of a virulent m. haemolytica serotype a1 genome ... | 2007 | 17240088 |
| cytochemical and ultrastructural study of anoikis and secondary necrosis in enterocytes detached in vivo. | detachment-induced apoptosis of enterocytes (anoikis) has not been investigated in vivo. here we describe anoikis of fish enterocytes following detachment in a septicemia by photobacterium damselae subsp. piscicida, or following injection of its exotoxin. the in vivo study was complemented with an ex vivo time-lapse analysis using conditions duplicating the in vivo situation. linings of enterocytes detached from intestine mucosa dissociate into isolated enterocytes which undergo caspase 3-mediat ... | 2007 | 17245641 |
| development of a novel pcr assay specific for riemerella anatipestifer. | riemerella anatipestifer is a significant pathogen of waterfowl and turkeys. due to their similar ecology and morphological and cultural characteristics it is important to differentiate r. anatipestifer infections from those caused by pasteurella multocida. present study describes a novel pcr assay that is capable of rapid and species-specific identification of r. anatipestifer from bacterial cultures. | 2007 | 17257252 |
| association of bovine respiratory disease with clinical status and acute phase proteins in calves. | eighty-four calves with respiratory disease from 18 herds in different parts of finland were chosen for a study evaluating the capacity of different respiratory pathogens to cause changes in different acute phase protein concentrations, white blood cell (wbc) count and clinical signs. the selected acute phase proteins were fibrinogen, haptoglobin, serum amyloid-a, lipopolysaccharide binding protein and alpha1-acid glycoprotein. from each calf, a paired blood sample was obtained for serological s ... | 2007 | 17258318 |
| comparative functional genomic analysis of pasteurellaceae adhesins using phage display. | the pasteurellaceae contain a number of important animal pathogens. although related, the various members of this family cause a diversity of pathology in a wide variety of organ systems. adhesion is an important virulence factor in bacterial infections. surprisingly little is known about the adhesins of the pasteurellaceae. to attempt to identify the genes coding for adhesins to some key components of the hosts extracellular matrix molecules, phage display libraries of fragmented genomic dna fr ... | 2007 | 17258409 |
| pasteurella multocida urinary tract infection in a pediatric patient with end-stage renal disease. | pasteurella multocida is a gram-negative bacillus that is part of the normal oral flora of cats and dogs. most infections involving p. multocida are soft tissue infections after animal bites or scratches. we present a case of p. multocida urinary tract infection in a 13-year-old boy with end-stage renal disease receiving peritoneal dialysis. he was successfully treated with intravenous ampicillin-sulbactam followed by oral amoxicillin-clavulanate. thirteen additional cases of p. multocida urinar ... | 2007 | 17259887 |
| using capacitance measurements as the detection method in antigen-containing layer-by-layer films for biosensing. | the layer-by-layer technique is employed here to immobilize antigen-containing liposomes, so-called proteoliposomes, onto au-interdigitated substrates, which are capable of molecular recognition of anti-pasteurellosis antibodies. detection was carried out using a novel strategy entirely based upon capacitance measurements, and to enhance sensitivity, we combine the response of three different sensing units in a similar procedure used for taste sensors. with the three-electrode array immunoglobul ... | 2007 | 17263514 |
| acute tissue-type plasminogen activator release in human microvascular endothelial cells: the roles of galphaq, plc-beta, ip3 and 5,6-epoxyeicosatrienoic acid. | the acute physiologic release of tissue-type plasminogen activator (t-pa) from the endothelium is critical for vascular homeostasis. this process is prostacyclin- and nitric oxide (no)-independent in humans. it has been suggested that calcium signaling and endothelial-derived hyperpolarizing factors (edhf) may play a role in t-pa release. g-protein-coupled receptor-dependent calcium signaling is typically galphaq-dependent. edhfs have been functionally defined and in various tissues are believed ... | 2007 | 17264956 |
| cloning of the gene and characterization of the enzymatic properties of the monomeric alkaline phosphatase (phox) from pasteurella multocida strain x-73. | we have identified a new phox gene encoding the monomeric alkaline phosphatase from pasteurella multocida x-73. this gene was not found in the published genome sequence of pasteurella multocida pm70. characterization of the recombinant phox of pasteurella multocida x-73 showed that it is a monomeric enzyme, activated by ca(2+) and possibly secreted by the tat pathway. these features distinguish phosphatases of the phox family from those of the phoa family. all proteins of the phox family were fo ... | 2007 | 17156125 |
| new consensus guidelines from the clinical and laboratory standards institute for antimicrobial susceptibility testing of infrequently isolated or fastidious bacteria. | the clinical and laboratory standards institute (clsi) recently published a new laboratory guideline for antimicrobial susceptibility testing of infrequently encountered or fastidious bacteria not covered in previous clsi publications. the organisms include aeromonas species, bacillus species, and vibrio species that may cause infections following environmental exposure. fastidious organisms that may cause endocarditis or medical device infections include abiotrophia and granulicatella species; ... | 2007 | 17173232 |
| quantitative continuous assay for hyaluronan synthase. | a rapid, continuous, and convenient three-enzyme coupled uv absorption assay was developed to quantitate the glucuronic acid and n-acetylglucosamine transferase activities of hyaluronan synthase from pasteurella multocida (pmhas). activity was measured by coupling the udp produced from the pmhas-catalyzed transfer of udp-glcnac and udp-glcua to a hyaluronic acid tetrasaccharide primer with the oxidation of nadh. using a fluorescently labeled primer, the products were characterized by gel electro ... | 2007 | 17173853 |
| modulation of host cell gene expression through activation of stat transcription factors by pasteurella multocida toxin. | the pasteurella multocida toxin (pmt) is highly mitogenic and has potential carcinogenic properties. pmt causes porcine atrophic rhinitis that is characterized by bone resorption and loss of nasal turbinates, but experimental nasal infection also leads to excess proliferation of bladder epithelial cells. pmt acts intracellularly and activates phospholipase c-linked signals and mapk pathways via the heterotrimeric galpha(q) and galpha(12/13) proteins. we found that pmt induces activation of stat ... | 2007 | 17150962 |
| antimicrobial activity of cathelicidins bmap28, smap28, smap29, and pmap23 against pasteurella multocida is more broad-spectrum than host species specific. | the antimicrobial activity of linear, cationic alpha-helical peptides from cattle (bmap28), sheep (smap28 and smap29), and pigs (pmap23) were assessed to determine if activity was selective for pasteurella multocida from a particular animal species or broad-spectrum against all p. multocida tested. the antimicrobial activities of synthetic peptides were determined for p. multocida isolated from cattle (10 isolates), sheep (10 isolates), and pigs (10 isolates) in a broth microdilution assay. all ... | 2007 | 16997510 |
| apoptosis in malignant glioma cells triggered by the temozolomide-induced dna lesion o6-methylguanine. | methylating drugs such as temozolomide (tmz) are widely used in the treatment of brain tumours (malignant gliomas). the mechanism of tmz-induced glioma cell death is unknown. here, we show that malignant glioma cells undergo apoptosis following treatment with the methylating agents n-methyl-n'-nitro-n-nitrosoguanidine (mnng) and tmz. cell death determined by colony formation and apoptosis following methylation is greatly stimulated by p53. transfection experiments with o(6)-methylguanine-dna met ... | 2007 | 16819506 |
| phylogenetic diversity of pasteurellaceae and horizontal gene transfer of leukotoxin in wild and domestic sheep. | wild and domestic animal populations are known to be sources and reservoirs of emerging diseases. there is also a growing recognition that horizontal genetic transfer (hgt) plays an important role in bacterial pathogenesis. we used molecular phylogenetic methods to assess diversity and cross-transmission rates of pasteurellaceae bacteria in populations of bighorn sheep, dall's sheep, domestic sheep and domestic goats. members of the pasteurellaceae cause an array of deadly illnesses including ba ... | 2007 | 16635591 |
| structural analysis of sialyltransferase pm0188 from pasteurella multocida complexed with donor analogue and acceptor sugar. | pm0188 is a newly identified sialyltransferase from p. multocida which transfers sialic acid from cytidine 5'-monophosphonuraminic acid (cmp-neuac) to an acceptor sugar. although sialyltransferases are involved in important biological functions like cell-cell recognition, cell differentiation and receptor-ligand interactions, little is known about their catalytic mechanism. here, we report the x-ray crystal structures of pm0188 in the presence of an acceptor sugar and a donor sugar analogue, rev ... | 2008 | 18304450 |
| associations between pathogens in healthy pigs and pigs with pneumonia. | the aim of this study was to evaluate the associations between different pathogens in the development of pneumonia and bronchopneumonia in pigs. samples of bronchoalveolar lavage fluid from 100 pigs showing no clinical signs and 239 pigs with clinical signs of respiratory disease were examined for mycoplasma hyopneumoniae, mycoplasma hyorhinis, us-type porcine respiratory and reproductive syndrome virus (prrsv), eu-type prrsv, porcine circovirus type 2 (pcv-2), influenza virus type a, alpha-haem ... | 2008 | 18310558 |
| genomic and functional analysis of icepdaspa1, a fish-pathogen-derived sxt-related integrating conjugative element that can mobilize a virulence plasmid. | integrating conjugative elements (ices) are self-transmissible mobile elements that transfer between bacteria via conjugation and integrate into the host chromosome. sxt and related ices became prevalent in asian vibrio cholerae populations in the 1990s and play an important role in the dissemination of antibiotic resistance genes in v. cholerae. here, we carried out genomic and functional analyses of icepdaspa1, an sxt-related ice derived from a spanish isolate of photobacterium damselae subsp. ... | 2008 | 18326579 |
| respiratory disease in lambs. | 2008 | 18326850 | |
| evaluation of different api systems for identification of porcine pasteurella multocida isolates. | an exhaustive biochemical characterisation of 60 porcine pasteurella multocida clinical isolates recovered from lesions indicative of pneumonia, previously confirmed by pcr and all belonging to the capsular serogroup a, was performed by means of four commercial systems. the api 20ne correctly identified almost all isolates (95%), but only 60% could be ascribed to this species by the api 20e method. the high diversity exhibited by the api 50chb/e system, with six different patterns, does not advi ... | 2008 | 18343468 |
| pasteurella multocida septic shock after a cat scratch in an elderly otherwise healthy woman: a case report. | pasteurella multocida, a gram-negative coccobacillus, is a commensal in the nasopharynx of many animals. p. multocida infections most commonly involve the skin, soft tissues, and respiratory tract, particularly in immunosuppressed patients. the present case illustrates a severe articular infection caused by this bacterium, leading to septic shock, in an elderly, otherwise healthy woman, after a simple scratch of a cat. | 2008 | 18358966 |
| [pathology of inoculation]. | 2008 | 18361288 | |
| the c3 domain of pasteurella multocida toxin is the minimal domain responsible for activation of gq-dependent calcium and mitogenic signaling. | the large 1285-amino-acid protein toxin from pasteurella multocida (pmt) is a multifunctional single-chain polypeptide that binds to and enters eukaryotic cells and acts intracellularly to promote g(q) and g(12/13) protein-dependent calcium and mitogenic signal transduction. previous studies indicated that the intracellular activity domain responsible for pmt action was located within the c-terminal 600-700 amino acids. in this study, we have exogenously expressed a series of n- and c-terminal p ... | 2008 | 18369188 |
| protein kinase d links gq-coupled receptors to camp response element-binding protein (creb)-ser133 phosphorylation in the heart. | many growth regulatory stimuli promote camp response element-binding protein (creb) ser(133) phosphorylation, but the physiologically relevant creb-ser(133) kinase(s) in the heart remains uncertain. this study identifies a novel role for protein kinase d (pkd) as an in vivo cardiac creb-ser(133) kinase. we show that thrombin activates a pkcdelta-pkd pathway leading to creb-ser(133) phosphorylation in cardiomyocytes and cardiac fibroblasts. alpha(1)-adrenergic receptors also activate a pkcdelta-p ... | 2008 | 18378685 |
| co-infection of respiratory bacterium with severe acute respiratory syndrome coronavirus induces an exacerbated pneumonia in mice. | sars-cov grows in a variety of tissues that express its receptor, although the mechanism for high replication in the lungs and severe respiratory illness is not well understood. we recently showed that elastase enhances sars-cov infection in cultured cells, which suggests that sars development may be due to elastase-mediated, enhanced sars-cov infection in the lungs. to explore this possibility, we examined whether co-infection of mice with sars-cov and pp, a low-pathogenic bacterium which elici ... | 2008 | 18380809 |
| atrophic rhinitis vaccine composition triggers different serological profiles that do not correlate with protection. | atrophic rhinitis (ar) is a widespread and economically important disease of swine caused by bordetella bronchiseptica and pasteurella multocida. it can be controlled by vaccination. this study investigates the effect of altering the composition (adjuvants and/or addition of formalin-inactivated p. multocida toxin, fpmt) of conventional vaccines on the serological profile and on protection against ar in swine. a significantly higher b. bronchiseptica specific antibody titre was detected for vacc ... | 2008 | 18401954 |
| protection of chickens from fowl cholera by vaccination with recombinant adhesive protein of pasteurella multocida. | the recombinant adhesive protein (rcp39) of pasteurella multocida strain p-1059 (serovar a:3) was prepared and purified with a hybrid condition of affinity chromatography. the rcp39 was highly protective for chickens from fowl cholera by challenge-exposure with parental strain p-1059 or heterologous strain x-73 (serovar a:1) compared to various kind of vaccines. sixteen groups of ten chickens each were subcutaneously inoculated twice with 100, 200 or 400 microg proteins of rcp39, native cp39, na ... | 2008 | 18403068 |
| magnetic resonance imaging features of spinal epidural empyema in five dogs. | spinal epidural empyema is defined an accumulation of purulent material in the epidural space of the vertebral canal. spinal epidural empyema should be considered as a differential diagnosis in dogs with pyrexia, spinal pain, and rapidly progressing myelopathy. magnetic resonance (mr) imaging is the imaging test of choice in humans. here, we describe the mr imaging features of five dogs with confirmed spinal epidural empyema. the epidural lesions appeared as high or mixed signal masses in t2-wei ... | 2008 | 18418993 |
| upgrading mouse health and welfare: direct benefits of a large-scale rederivation programme. | we report the outcome of a 30-month programme to rederive 310 specific pathogen-free mouse strains to populate a new individually ventilated cage barrier facility at the mary lyon centre (mlc), medical research council (mrc) harwell. the mice were rederived in a self-contained quarantine suite and embryo-recipient females were health-screened to assess microbiological status, before moving their offspring into the new facility. the mlc currently houses approximately 49,000 mice in about 9750 cag ... | 2008 | 18435871 |
| using amplified fragment length polymorphism analysis to differentiate isolates of pasteurella multocida serotype 1. | avian cholera, an infectious disease caused by the bacterium pasteurella multocida, kills thousands of north american wild waterfowl annually. pasteurella multocida serotype 1 isolates cultured during a laboratory challenge study of mallards (anas platyrhynchos) and collected from wild birds and environmental samples during avian cholera outbreaks were characterized using amplified fragment length polymorphism (aflp) analysis, a whole-genome dna fingerprinting technique. comparison of the aflp p ... | 2008 | 18436655 |
| epidemic pasteurellosis in a bighorn sheep population coinciding with the appearance of a domestic sheep. | a pneumonia epidemic reduced bighorn sheep (ovis canadensis) survival and recruitment during 1997-2000 in a population comprised of three interconnected wintering herds (kenosha mountains, sugarloaf mountain, twin eagles) that inhabited the kenosha and tarryall mountain ranges in central colorado, usa. the onset of this epidemic coincided temporally and spatially with the appearance of a single domestic sheep (ovis aires) on the sugarloaf mountain herd's winter range in december 1997. although o ... | 2008 | 18436671 |
| [characteristics and immunogenicity of the n-terminal and c-terminal recombinants of pasteurella multocida toxin]. | in this study, five fragments of recombinant subunit pasteurella multocida toxin (pmt) were constructed. only pet28a-n1518 and pet28b-c2115 could be expressed efficiently in escherichia coli. the molecular weight of the fusion proteins was 57 kda and 78 kda .western blot confirmed that the two proteins could specifically react with antiserum against pasteurella multocida toxin. no mice died after the intraperitoneal administration of these two proteins with the dose of 200 microg per mouse, but ... | 2008 | 18438004 |
| [celulitis in an 88-year old woman]. | 2008 | 18457640 | |
| identification of pasteurella multocida chaps-soluble outer membrane proteins. | fowl cholera continues to be of concern to the poultry industry, especially for turkey growers. this disease costs the turkey industry millions of dollars annually. in order to develop improved live attenuated vaccines or subunit vaccines, the outer-membrane proteins of pasteurella multocida were examined with the use of proteomics. of the 11 proteins total present in an outer-membrane subfraction of p. multocida, four additional proteins were identified, completing the composition of the deterg ... | 2008 | 18459313 |
| effects of subminimum inhibitory concentrations of antibiotics on the pasteurella multocida proteome: a systems approach. | to identify key regulators of subminimum inhibitory concentration (sub-mic) antibiotic response in the pasteurella multocida proteome, we applied systems approaches. using 2d-lc-esi-ms(2), we achieved 53% proteome coverage. to study the differential protein expression in response to sub-mic antibiotics in the context of protein interaction networks, we inferred p. multocida pm70 protein interaction network from orthologous proteins. we then overlaid the differential protein expression data onto ... | 2008 | 18464924 |
| transmission of pathogenic respiratory bacteria to specific pathogen free pigs at slaughter. | the purpose of this study was to evaluate the transmission of pathogenic respiratory bacteria to thirteen 5-month-old specific pathogen free (spf) pigs, during the slaughtering process in a commercial slaughterhouse. before transportation, the spf pigs and the lorry were checked to confirm the absence of pathogenic respiratory bacteria. nine spf pigs (group 1) were in contact in a conventional slaughterhouse with finishing pigs, during 4h before slaughtering. four spf pigs (group 2) were slaught ... | 2008 | 18242013 |
| what is your diagnosis? epidural empyema. | 2008 | 18251990 | |
| quest for the diagnosis. case 1: a neonatal zoonosis. neonatal pasteurella multocida septicaemia. | 2008 | 18254915 | |
| experimental infection studies of pasteurella pneumotropica and v-factor dependent pasteurellaceae for f344-rnu rats. | to investigate the pathogenicities of p. pneumotropica (pp) and v-factor dependent pasteurellaceae (vfdp) in immunodeficient rats, experimental infections of f344-rnu rats were performed using 3 strains (atcc 35149, cnp 160 and rpz) of pp and 4 strains (v6, v7, v8 and v9) of vfdp. four animals per experimental group were inoculated twice on day 0 and post-inoculation day (pid) 14 with bacterial suspension intranasally. two animals from each group were sacrificed on pid 60 and 120, and examined. ... | 2008 | 18256519 |
| extracellular polysaccharides produced by cooling water tower biofilm bacteria and their possible degradation. | the extracellular polymers (eps) of biofilm bacteria that can cause heat and mass transfer problems in cooling water towers in the petrochemical industry were investigated. in addition, these microorganisms were screened for their ability to grow and degrade their own eps and the eps of other species. twelve bacteria producing the most eps were isolated from cooling water towers and characterized biochemically by classic and commercial systems. these were species of pseudomonas, burkholderia, ae ... | 2008 | 18256966 |
| evaluation of pyelonephritis in culled indoor and outdoor high parity sows. | the present trial was conducted in hungary in neighboring large indoor and outdoor pig production units, belonging to the same breeding company. rejected kidneys from 201 (out of 241; 83.4%) outdoor, and 191 (out of 512, 37.3%) indoor high parity sows, with previous history of recidiving postparturient fever and excessive postparturient vulvovaginal discharge were gross pathologically bacteriologically, and histologically evaluated. all rejected kidneys revealed chronic pyelonephritis. in outdoo ... | 2008 | 18265757 |
| maternal undernutrition and the ovine acute phase response to vaccination. | the acute phase response is the immediate host response to infection, inflammation and trauma and can be monitored by measuring the acute phase proteins (app) such as haptoglobin (hp) or serum amyloid a (saa). the plane of nutrition during pregnancy is known to affect many mechanisms including the neuroendocrine and neuroimmune systems in neonatal animals but effects on the app are unknown. to investigate this phenomenon the serum concentration of hp and saa was initially determined in non-stimu ... | 2008 | 18197966 |
| pasteurella multocida in scavenging family chickens and ducks: carrier status, age susceptibility and transmission between species. | pasteurella multocida causes fowl cholera, a highly contagious and severe disease in chickens and water fowls. the disease is not well described in less intensive production systems, including scavenging family poultry production in developing countries. p. multocida was isolated from 25.9% of healthy-looking ducks and 6.2% of chickens from free-range family poultry farms and at slaughter slabs at market. on experimental infection with 1.2 to 2.0 x 10(8) organisms of the p. multocida type strain ... | 2008 | 18202950 |
| microbial contamination of dental unit waterlines in istanbul, turkey. | the water used in dental unit waterlines (duwls) acts as a coolant for the high-speed equipment and as an irrigant during dental treatments. there are kind of water tanks. duwls provide a favorable environment for microbial biofilm and multiplation primarily due to the high surface in the tubing and the character of fluid dynamics in narrow, smooth-walled waterlines. biofilms can harbour opportunist pathogens such as legionella sp., pseudomonas sp. several studies have shown that duwls have high ... | 2008 | 18210208 |