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ensifer shofinae sp. nov., a novel rhizobial species isolated from root nodules of soybean (glycine max).two bacterial strains isolated from root nodules of soybean were characterized phylogenetically as members of a distinct group in the genus ensifer based on 16s rrna gene comparisons. they were also verified as a separated group by the concatenated sequence analyses of reca, atpd and glnii (with similarities ≤93.9% to the type strains for defined species), and by the average nucleotide identities (ani) between the whole genome sequence of the representative strain ccbau 251167(t) and those of th ...201728209394
transcription factors pverf15 and pvmtf-1 form a cadmium stress transcriptional pathway.in plants, cadmium (cd)-responsive transcription factors are key downstream effectors of cd stress transcriptional pathways, which are capable of converging cd stress signals through triggering the expression of cd detoxification genes. however, the upstream transcriptional regulatory pathways that modulate their responses to cd are less clear. previously, we identified the bean (phaseolus vulgaris) metal response element-binding transcription factor1 (pvmtf-1) that responds to cd and confers cd ...201728073984
prevalence of igg-mediated food intolerance among patients with allergic symptoms.food intolerance mediated by food specific igg antibodies has been implicated in a variety of disorders.201727920409
impact of rhizobial inoculation and reduced n supply on biomass production and biological n2 fixation in common bean grown hydroponically.testing rhizobial inoculation of common bean (phaseolus vulgaris l.) in hydroponics enables accurate quantification of biological n2 fixation (bnf) and provides information about the potential of reducing inorganic n fertilizer use. in view of this background, common bean grown on pumice was inoculated with rhizobium tropici ciat899 (rt) and supplied with either full-n (total nitrogen 11.2 mmol l(-1) ), 1/3 of full-n or n-free nutrient solution (ns). bnf was quantified at the early pod-filling s ...201728071798
capulavirus and grablovirus: two new genera in the family geminiviridae.geminiviruses are plant-infecting single-stranded dna viruses that occur in most parts of the world. currently, there are seven genera within the family geminiviridae (becurtovirus, begomovirus, curtovirus, eragrovirus, mastrevirus, topocuvirus and turncurtovirus). the rate of discovery of new geminiviruses has increased significantly over the last decade as a result of new molecular tools and approaches (rolling-circle amplification and deep sequencing) that allow for high-throughput workflows. ...201728213872
comparison of effectiveness of 5'-regulatory sequences in transplastomic tobacco chloroplasts.the development of tools which ensure the desired level of transgene expression in plastids is a prerequisite for the effective utilization of these plant organelles for the deployment of bioactive proteins. high-level accumulation of target proteins is considered as a positive feature of transplastomic plants, but excessive accumulation of foreign proteins may have deleterious effects on host plants. on the other hand, expression at low levels can result in ineffective phenotypes. we compared t ...201727565642
seed-specific expression and analysis of recombinant anti-her2 single-chain variable fragment (scfv-fc) in arabidopsis thaliana.antibodies to human epidermal growth factor receptor 2 (her2) are a key element of breast cancer therapy; however, they are expensive to produce and their availability is limited. a seed-specific expression system can be used to produce recombinant proteins. we report a seed-specific expression system for the manufacture of anti-her2 scfv-fc in arabidopsis thaliana, driven by the phaseolus vulgaris β-phaseolin promoter. recombinant anti-her2 scfv-fc was successfully and specifically expressed in ...201728286176
evolution and structural diversification of nictaba-like lectin genes in food crops with a focus on soybean (glycine max).the nictaba family groups all proteins that show homology to nictaba, the tobacco lectin. so far, nictaba and an arabidopsis thaliana homologue have been shown to be implicated in the plant stress response. the availability of more than 50 sequenced plant genomes provided the opportunity for a genome-wide identification of nictaba-like genes in 15 species, representing members of the fabaceae, poaceae, solanaceae, musaceae, arecaceae, malvaceae and rubiaceae. additionally, phylogenetic relations ...201728087663
nucleotide sequence of phaseolus vulgaris l. alcohol dehydrogenase encoding cdna and three-dimensional structure prediction of the deduced protein.common beans (phaseolus vulgaris l.) are widely consumed as a source of proteins and natural products. however, its yield needs to be increased. in line with the agenda of phaseomics (an international consortium), work of expressed sequence tags (ests) generation from bean pods was initiated. altogether, 5972 ests have been isolated. alcohol dehydrogenase (ad) encoding gene cdna was a noticeable transcript among the generated ests. this ad is an important enzyme; therefore, to understand more ab ...201725829796
circadian periodic response of phaseolus vulgaris l. to 2,4-dichlorophenoxyacetic acid.the susceptibility of bean plants, phaseolus vulgaris l., cv. executive, to 2,4-dichlorophentoxyacetic acid (2,4-d) appeared to depend upon the time of application. oscillations in response to 2,4-d were evident in plants subjected to conditions of alternating light and dark spans, continuous illumination or darkness. the fresh and dry weight of plant material was generally less when 2,4-d was applied to plants near the later portions of the light span.2017976022
from jelly beans to kidney beans: what diabetes educators should know about the glycemic index. 201712212338
determination of clethodim and its oxidation metabolites in crops by liquid chromatography with confirmation by lc/ms.a method was developed for determination of the herbicide clethodim (c0) and its oxidation metabolites clethodim sulfoxide (c1) and clethodim sulfone (c2) in agricultural products. upon extraction, both c0 and c1 were oxidized to c2 by m-chloroperoxybenzoic acid, and c2 was determined by liquid chromatography (lc). the c2 peak was confirmed by liquid chromatography/mass spectrometry (lc/ms) with electrospray ionization (esi). recoveries of c0 from radish, tomato, onion, sweet potato, kidney bean ...201711501920
extraction and isolation of soluble and insoluble fiber fractions from the pinto bean (phaseolus vulgaris). 20176256421
comparison and assessment of the difference in total dietary fiber in cooked dried legumes as determined by five methods.dried beans (kidney and great northern) and dried peas (chick, green, and yellow) were cooked according to package instructions. total dietary fiber (tdf) was measured by the mongeau (aoac 992.16), prosky (aoac 985.29), and lee (aoac 991.43) methods (a, b, and c, respectively). nonstarch polysaccharides (nsp) were measured by the englyst gas-chromatographic method that included dimethyl sulfoxide treatment, and the lignin measured separately was added to nsp (method d). tdf was also measured by ...20177950419
effectiveness of electrolyzed oxidizing water treatment in removing pesticide residues and its effect on produce quality.this study evaluated the effects of electrolyzed oxidizing (eo) water treatment on the removal of pesticide residues (diazinon, cyprodinil and phosmet) from spinach, snap beans and grapes, and the effect on produce quality. high available chlorine content (acc) and long treatment time of eo water resulted in high pesticide removals. up to 59.2, 66.5 and 37.1% of diazinon; 43.8, 50.0 and 31.5% of cyprodinil; 85.7, 73.0 and 49.4% of phosmet; were removed from spinach, snap beans and grapes, respec ...201828873606
determination of heavy metals in canned fruits and vegetables sold in jordan market.in this study, the concentrations of selected heavy metals including pb, zn, cr, ni, cu, as, and cd in different brands of canned vegetables and fruits including canned tomato sauce (ketchup), canned green beans, canned whole carrots, and canned juice (pineapple) imported to jordanian market were determined by acid digestion and atomic absorption spectroscopy. samples were collected from popular jordanian markets, irbid city, northern jordan (11 samples of each type). the metal concentrations in ...201829103121
reduction in root secondary growth as a strategy for phosphorus acquisition.we tested the hypothesis that reduced root secondary growth of dicotyledonous species improves phosphorus acquisition. functional-structural modeling in simroot indicates that, in common bean (phaseolus vulgaris), reduced root secondary growth reduces root metabolic costs, increases root length, improves phosphorus capture, and increases shoot biomass in low-phosphorus soil. observations from the field and greenhouse confirm that, under phosphorus stress, resource allocation is shifted from seco ...201829118249
a closer look to cell structural barriers affecting starch digestibility in beans.isolated bean cells were used to understand the contribution of cell wall and cytoplasmic matrix on starch digestibility. cotyledon cells were treated enzymatically and mechanically to reduce the level of cell intactness. sem and chemical characterization revealed that enzymatic treatment modified cell wall thickness and porosity without altering the cytoplasmic matrix, whereas mechanical treatment completely disrupted cell structure. decreasing cell intactness increased the rate but not the ext ...201829254064
plant extract-mediated green silver nanoparticles: efficacy as soil conditioner and plant growth promoter.recently, concerns have been raised regarding the ultimate fate of silver nanoparticles (snps) after their release into the environment. in this study, the environmental feasibility of plant leaf (thuja occidentalis) extract-mediated green snps (gsnps) was assessed in terms of their effects on soil physicochemical properties and crop growth in comparison to conventionally synthesized silver nanoparticles (csnps). upon application of gsnps, soil ph shifted toward neutrality, and substantial incre ...201829247955
transcriptome - scale characterization of salt responsive bean tcp transcription factors.teosinte-branched1/cycloidea/pcf (tcp) proteins are important regulators of growth and developmental processes including branching, floral organ morphogenesis and leaf growth as well as stress response. this study identified 27 tcp genes of phaseolus vulgaris (common bean), which were divided into three clusters based on phylogenetic relationship. in addition, this study showed that some of tcp genes such as pvul-tcp-4 and pvul-tcp-15 located on chromosomes 3 and 7, pvul-tcp-7 and pvul-tcp-20 lo ...201829129811
responses of two kidney bean (phaseolus vulgaris) cultivars to the combined stress of sulfur deficiency and cadmium toxicity.plants suffer from combined stress of sulfur deficiency and cadmium toxicity in some agricultural lands. however, little is known about the reaction in plants, such as responses in antioxidant enzymes and non-protein thiol compounds, to such combined stress. therefore, in this study, four treatments, s-sufficiency (ts-cd), s-deficiency (t-s-cd), cd stress (ts+cd) and combined stress of s-deficiency and cd stress (t-s+cd), were set up to investigate (1) the effects of sulfur deficiency or sulfur ...201829188540
impact of conventional and integrated management systems on the water-soluble vitamin content in potatoes, field beans, and cereals.the reduction of the environmental footprint of crop production without compromising crop yield and their nutritional value is a key goal for improving the sustainability of agriculture. in 2009, the balruddery farm platform was established at the james hutton institute as a long-term experimental platform for cross-disciplinary research of crops using two agricultural ecosystems. crops representative of uk agriculture were grown under conventional and integrated management systems and analyzed ...201829257861
new research highlights: impact of chronic ingestion of white kidney beans (phaseolus vulgaris l. var. beldia) on small-intestinal disaccharidase activity in wistar rats.the chronic ingestion of raw or undercooked kidney beans (phaseolus vulgaris l.) is involved in the pathogenesis of multiple organ dysfunction; the underlying mechanisms are still poorly understood. the objective of this study was to assess the gavage effects of a raw beldia bean variety on the brush border disaccharidase activities in the jejunal mucosa of wistar rats. twenty young adult male rats were randomly assigned into 2 groups of 10 rats each: control, rats were gavaged with 300 mg of a ...201829270365
the expression of an exogenous acc deaminase by the endophyte serratia grimesii bxf1 promotes the early nodulation and growth of common bean.ethylene acts as an inhibitor of the nodulation process of leguminous plants. however, some bacteria can decrease deleterious ethylene levels by the action of the enzyme 1-aminocyclopropane-1-carboxylate (acc) deaminase which degrades acc, the ethylene precursor in all higher plants. co-inoculation of rhizobia with endophytes enhances the rhizobial symbiotic efficiency with legumes, improving both nodulation and nitrogen fixation. however, not much is understood about the mechanisms employed by ...201829327464
expression profiling of the dolichos lablab lectin during germination and development of the seed.the temporal expression of the field bean (dolichos lablab) galactose specific lectin, dll-ii, during germination, post-germination and seed development was evaluated using native-page followed by activity staining, immunodetection and quantitative real time pcr (qpcr). a rapid and steep decline in the polyphenol oxidase (ppo) and hemagglutinating activity during the initial stages of germination, which did not correlate with the slow decline in total protein was observed. during post germinatio ...201829324242
conservation and genetic characterisation of common bean landraces from cilento region (southern italy): high differentiation in spite of low genetic diversity.since its introduction from central-south america to italy almost 500 years ago, the common bean (phaseolus vulgaris l.) was largely cultivated across the peninsula in hundreds of different landraces. however, globalisation and technological modernisation of agricultural practices in the last decades promoted the cultivation of few varieties at the expense of traditional and local agro-ecotypes, which have been confined to local markets or have completely disappeared. the aim of this study was t ...201829030763
influence of resistance breeding in common bean on rhizosphere microbiome composition and function.the rhizosphere microbiome has a key role in plant growth and health, providing a first line of defense against root infections by soil-borne pathogens. here, we investigated the composition and metabolic potential of the rhizobacterial community of different common bean (phaseolus vulgaris) cultivars with variable levels of resistance to the fungal root pathogen fusarium oxysporum (fox). for the different bean cultivars grown in two soils with contrasting physicochemical properties and microbia ...201829028000
spatial and temporal scales of range expansion in wild phaseolus vulgaris.the wild progenitor of common-bean has an exceptionally large distribution from northern mexico to northwestern argentina, unusual among crop wild progenitors. this research sought to document major events of range expansion that led to this distribution and associated environmental changes. through the use of genotyping-by-sequencing (∼20,000 snps) and geographic information systems applied to a sample of 246 accessions of wild phaseolus vulgaris, including 157 genotypes of the mesoamerican, 77 ...201829069389
mass spectrometry-based phytochemical screening for hypoglycemic activity of fagioli di sarconi beans (phaseolus vulgaris l.).the present study deals with the evaluation of antidiabetic activities of fagioli di sarconi beans (phaseolus vulgaris), including 21 ecotypes protected by the european union with the mark pgi (i.e., protected geographical indication), and cultivated in basilicata (southern italy). for this purpose, α-glucosidase and α-amylase assays were assessed; among all bean ecotypes, the tight green seed colour of verdolino extracts exhibited the highest α-glucosidase and α-amylase inhibitory activity with ...201829037720
inhibitory effects of cinnamon and clove essential oils on mold growth on baked foods.this study evaluated the minimum inhibition concentration (mic) and minimum lethal concentration (mlc) of cinnamon and clove essential oils against mold growth on green bean cake and finger citron crisp cake, and also examined the effects of these two essential oils and their application methods on the shelf life of the baked products in normal and vacuum packages by accelerated storage test. the results showed that the mic of cinnamon and clove essential oils against molds were 0.21-0.83 and 0. ...201828946351
physicochemical properties of nixtamalized black bean (phaseolus vulgaris l.) flours.the aim of this work was to study the effect of three nixtamalization processes using wood ashes, ca(oh)2, and cacl2 on the physicochemical properties of beans. raw beans had c-type starch, 10.10% resistant starch (rs), and two dsc endotherms: 1) starch gelatinization, and 2) melting of amylose-lipid complexes plus protein denaturation. nixtamalization increased the ca and fe content, decreased the rs content to 4.19-4.43%, and produced starch retrogradation. dsc and ft-ir analysis of the flours ...201828946298
dissipation rate study and pre-harvest intervals calculation of imidacloprid and oxamyl in exported egyptian green beans and chili peppers after pestigation treatment.two quechers-based methods were developed and validated, using liquid chromatography-tandem mass spectrometric detection, in order to accurately determine residues of imidacloprid and oxamyl in green beans and chili peppers after treatment via irrigation system under field conditions in egyptian farms. the validation included experiments for specificity, linearity, trueness, precision, matrix effect and limits of detection and quantification according to european commission standards. the dissip ...201828946222
allergy to peanut, soybean, and other legumes: recent advances in allergen characterization, stability to processing and ige cross-reactivity.peanut and soybean are members of the leguminosae family. they are two of the eight foods that account for the most significant food allergies in the united states and europe. allergic reactions to other legume species can be of importance in other regions of the world. the major allergens from peanut and soybean have been extensively analyzed and members of new protein families identified as potential marker allergens for symptom severity. important recent advances concerning their molecular pr ...201828944625
morphological, proteomic and metabolomic insight into the effect of cerium dioxide nanoparticles to phaseolus vulgaris l. under soil or foliar application.chemically synthesized nanoparticles (nps) are widely used in industry and concern over their impact on the environment is rising. in this study, greenhouse grown bean (phaseolus vulgaris l.) plants were treated with ceo2 nps suspensions at 0, 250, 500, 1000, and 2000mgl-1 either aerially by spraying or via soil application. at 15days after treatment, plants were analyzed for ce uptake, morphological and biochemical assays, as well as high-resolution mass spectrometry based metabolomics and prot ...201829066204
sequential sampling plans and economic injury levels for empoasca kraemeri on common bean crops at different technological levels.empoasca kraemeri is an important pest on common bean crops at different technological levels. however, for this pest on this crop, economic injury levels have not yet been determined and plan for sequential sampling plans has not yet been developed. thus, the objectives of this research were to develop e. kraemeri sequential sampling plans and to determine economic injury levels in the common bean at different crop technological levels.201828876534
neuronal connections of the central amygdalar nucleus with refeeding-activated brain areas in rats.following fasting, satiety is accompanied by neuronal activation in brain areas including the central amygdalar nucleus (cea). since cea is known to inhibit food intake, we hypothesized that cea contributes to the termination of meal during refeeding. to better understand the organization of this satiety-related circuit, the interconnections of the cea with refeeding-activated neuronal groups were elucidated using retrograde (cholera toxin-β subunit, ctb) and anterograde (phaseolus vulgaris leuc ...201828852859
prediction of canned black bean texture (phaseolus vulgaris l.) from intact dry seeds using visible/near infrared spectroscopy and hyperspectral imaging data.texture is a major quality parameter for the acceptability of canned whole beans. prior knowledge of this quality trait before processing would be useful to guide variety development by bean breeders and optimize handling protocols by processors. the objective of this study was to evaluate and compare the predictive power of visible and near infrared reflectance spectroscopy (visible/nirs, 400-2498 nm) and hyperspectral imaging (hypers, 400-1000 nm) techniques for predicting texture of canned bl ...201828585253
cookies elaborated with oat and common bean flours improved serum markers in diabetic rats.common beans have been associated with anti-diabetic effects, due to its high content of bioactive compounds. nevertheless, its consumption has decreased worldwide. therefore, there is an increasing interest in the development of novel functional foods elaborated with common beans. the aim of this study was to evaluate the anti-diabetic effect of oat-bean flour cookies, and to analyze its bioactive composition, using commercial oat-wheat cookies for comparative purposes.201828718519
is nitrogen accumulation in grain legumes responsive to growth or ontogeny?nitrogen (n) accumulation in legumes is one of the main determinants of crop yield. although n accumulation from symbiotic nitrogen fixation or n absorption from the soil has been widely investigated, there is no clear consensus on timing of the beginning of n accumulation and the termination of n accumulation and the physiological events that may be associated with these two events. the analyses conducted in this study aimed at identifying the determinant of n accumulation in two grain legume s ...201828786121
physiological traits of endornavirus-infected and endornavirus-free common bean (phaseolus vulgaris) cv black turtle soup.this study evaluated the physiological traits of eight lines of common bean (phaseolus vulgaris) cv. black turtle soup, four of which were double-infected with phaseolus vulgaris endornavirus 1 and phaseolus vulgaris endornavirus 2, and four of which were endornavirus-free. plants from all eight lines were morphologically similar and did not show statistically significant differences in plant height, wet weight, number of days to flowering and pod formation, pods per plant, pod thickness, seed s ...201829307088
computational investigation of small rnas in the establishment of root nodules and arbuscular mycorrhiza in leguminous plants.many small rnas have been confirmed to play important roles in the development of root nodules and arbuscular mycorrhiza. in this study, we carried out the identification of certain small rnas in leguminous plants (medicago truncatula, soybean, peanut and common bean), such as mirnas, trfs and srrnas, as well as the computational investigation of their regulations. thirty mirnas were predicted to be involved in establishing root nodules and mycorrhiza, and 12 of them were novel in common bean an ...201829305786
root environment is a key determinant of fungal entomopathogen endophytism following seed treatment in the common bean, phaseolus vulgaris.the common bean is the most important food legume in the world. we examined the potential of the fungal entomopathogens beauveria bassiana and metarhizium anisopliae applied as seed treatments for their endophytic establishment in the common bean. endophytic colonization in sterile sand:peat averaged ca. 40% higher for fungus treatments and ca. six times higher for volunteer fungi (other fungal endophytes naturally occurring in our samples), relative to sterile vermiculite. colonization by b. ba ...201829302156
foodborne botulism due to ingestion of home-canned green beans: two case reports.foodborne botulism is a life-threatening, rapidly progressive disease. it has an incidence of less than 10 cases per year in germany and mostly affects several previously healthy people at the same time. the only specific treatment is the administration of botulism antitoxin. according to the german guidelines administration of antitoxin is recommended only in the first 24 hours after oral ingestion of the toxin.201829301587
in bps1 downregulated roots, the bypass1 signal disrupts the induction of cortical cell divisions in bean-rhizobium symbiosis.bypass1 (bps1), which is a well-conserved gene in plants, is required for normal root and shoot development. in the absence of bps1 gene function, arabidopsis overproduces a mobile signalling compound (the bps1 signal) in roots, and this transmissible signal arrests shoot growth and causes abnormal root development. in addition to the shoot and root meristem activities, the legumes also possess transient meristematic activity in root cortical cells during rhizobium symbiosis. we explored the rol ...201829301366
quantile regression for genome-wide association study of flowering time-related traits in common bean.flowering is an important agronomic trait. quantile regression (qr) can be used to fit models for all portions of a probability distribution. in genome-wide association studies (gwas), qr can estimate snp (single nucleotide polymorphism) effects on each quantile of interest. the objectives of this study were to estimate genetic parameters and to use qr to identify genomic regions for phenological traits (days to first flower-dff; days for flowering-dtf; days to end of flowering-def) in common be ...201829300788
qtl mapping and transcriptome analysis of cowpea reveals candidate genes for root-knot nematode resistance.cowpea is one of the most important food and forage legumes in drier regions of the tropics and subtropics. however, cowpea yield worldwide is markedly below the known potential due to abiotic and biotic stresses, including parasitism by root-knot nematodes (meloidogyne spp., rkn). two resistance genes with dominant effect, rk and rk2, have been reported to provide resistance against rkn in cowpea. despite their description and use in breeding for resistance to rkn and particularly genetic mappi ...201829300744
quality of black beans as a function of long-term storage and moldy development: chemical and functional properties of flour and isolated protein.the aim of this study was to evaluate the effects of moisture content and storage temperature on the percentage of moldy and fermented beans, mycotoxins levels, phenolic acids content, pasting properties of whole flour, as well as functional and thermal properties of protein isolates from black beans stored for 12 months. beans stored under 14%/32 °c exhibited 16% of fermented grains, while at 17%/25 °c (42.3%) and 17%/32 °c (93.5%) of moldy plus fermented grains, named drastic conditions (dc). ...201829291875
toxicity of bifenthrin and mixtures of bifenthrin plus acephate, imidacloprid, thiamethoxam, or dicrotophos to adults of tarnished plant bug (hemiptera: miridae).to assess the toxicity of bifenthrin and four mixtures of insecticides to tarnished plant bug, we used an insecticide dip method of green bean to treat adults of a laboratory colony; mortality was assessed after 48 h. lc50s for imidacloprid, bifenthrin, acephate, thiamethoxam, and dicrotophos were 0.12, 0.39, 0.62, 0.67, and 3.96 ppm, respectively. lc75s for imidacloprid, bifenthrin, acephate, thiamethoxam, and dicrotophos were 0.61, 4.22, 5.10, 2.65, and 7.86 ppm, respectively. based on the lc5 ...201829361174
the common bean cok-4 and the arabidopsis fer kinase domain share similar function in plant growth and defense.receptor-like kinases are membrane proteins that can be shared by diverse signaling pathways. among them, the arabidopsis thaliana feronia (fer) plays a role in the balance between distinct signals to control growth and defense. we have found that cok-4, a putative kinase encoded in the common bean anthracnose resistance locus co-4 that is transcriptionally regulated during immune response, is highly similar to the kinase domain of fer. to assess whether cok-4 is a functional ortholog of fer, we ...201829352746
consumer acceptance comparison between seasoned and unseasoned vegetables.recent findings show that approximately 87% of the u.s. population fail to meet the vegetable intake recommendations, with unpleasant taste of vegetables being listed as the primary reason for this shortfall. in this study, spice and herb seasoning was used to enhance palatability of vegetables, in order to increase consumer acceptance. in total, 749 panelists were screened and recruited as specific vegetable likers of the vegetable being tested or general vegetable likers. four sessions were de ...201829337353
alpha-amylase, α-glucosidase and lipase inhibiting activities of polyphenol-rich extracts from six common bean cultivars of southern italy, before and after cooking.common beans (phaseolus vulgaris) are a good source of nutrients and phenolic compounds with versatile health benefits. polyphenol-rich extracts of six ecotypes of p. vulgaris were analysed to determine their phenolic profiles and assayed in vitro for inhibitory effects on digestive enzymes relevant to carbohydrates and lipids metabolism. the extracts inhibited enzyme activities in a dose-dependent manner. ic 50 values ranged from 69 ± 1.9 to 126 ± 3.2 μg/ml and from 107.01 ± 4.5 to 184.20 ± 5.7 ...201829336184
the role of smell, taste, flavour and texture cues in the identification of vegetables.it has been shown that the identification of many foods including vegetables based on flavour cues is often difficult. the effect of providing texture cues in addition to flavour cues on the identification of foods and the effect of providing taste cues only on the identification of foods have not been studied. the aim of this study was to assess the role of smell, taste, flavour and texture cues in the identification of ten vegetables commonly consumed in the netherlands (broccoli, cauliflower, ...201829108741
a transcriptome-snp-derived linkage map of apios americana (potato bean) provides insights about genome re-organization and synteny conservation in the phaseoloid legumes.we report a linkage map for apios americana and describe synteny with selected warm-season legumes. a translocation event in common bean and soybean is confirmed against apios and vigna species. apios (apios americana; "apios"), a tuberous perennial legume in the phaseoleae tribe, was widely used as a food by native americans. work in the last 40 years has led to several improved breeding lines. aspects of the pollination biology (complex floral structure and tripping mechanism) have made contro ...201829071392
characterization and molecular modeling of inositol 1,3,4 tris phosphate 5/6 kinase-2 from glycine max (l) merr.: comprehending its evolutionary conservancy at functional level.soybean genome encodes a family of four inositol 1,3,4 trisphosphate 5/6 kinases which belong to the atp-grasp group of proteins. inositol 1,3,4 trisphosphate kinase-2 (gmitpk2), catalyzing the atp-dependent phosphorylation of inositol 1,3,4 trisphosphate (ip3) to inositol 1,3,4,5 tetra phosphate or inositol 1,3,4,6 tetra phosphate, is a key enzyme diverting the flux of inositol phosphate pool towards phytate biosynthesis. although considerable research on characterizing genes involved in phytat ...201829354361
association of extracellular dntp utilization with a gmpap1-like protein identified in cell wall proteomic analysis of soybean roots.plant root cell walls are dynamic systems that serve as the first plant compartment responsive to soil conditions, such as phosphorus (p) deficiency. to date, evidence for the regulation of root cell wall proteins (cwps) by p deficiency remains sparse. in order to gain a better understanding of the roles played by cwps in the roots of soybean (glycine max) in adaptation to p deficiency, we conducted an itraq (isobaric tag for relative and absolute quantitation) proteomic analysis. a total of 53 ...201829329437
genetic and epigenetic divergence of duplicate genes in two legume species.soybean (glycine max) and common bean (phaseolus vulgaris) share a polyploidy event ~59 mya, followed by a glycine-specific wgd ~8-13 mya. duplicated genes were classified into five categories: singletons, dispersed, proximal, tandem or wgd/segmental and found strong correlations between gene category and functional annotation. photosynthesis and transcriptional regulation-related go terms were significantly over-represented in singletons and wgd genes, respectively, aligning with the gene balan ...201829314059
multi-scale measurements show limited soil greenhouse gas emissions in kenyan smallholder coffee-dairy systems.efforts have been made in recent years to improve knowledge about soil greenhouse gas (ghg) fluxes from sub-saharan africa. however, data on soil ghg emissions from smallholder coffee-dairy systems have not hitherto been measured experimentally. this study aimed to quantify soil ghg emissions at different spatial and temporal scales in smallholder coffee-dairy farms in murang'a county, central kenya. ghg measurements were carried out for one year, comprising two cropping seasons, using vented st ...201829348066
genome-wide association study reveals candidate genes influencing lipids and diterpenes contents in coffea arabica l.lipids, including the diterpenes cafestol and kahweol, are key compounds that contribute to the quality of coffee beverages. we determined total lipid content and cafestol and kahweol concentrations in green beans and genotyped 107 coffea arabica accessions, including wild genotypes from the historical fao collection from ethiopia. a genome-wide association study was performed to identify genomic regions associated with lipid, cafestol and kahweol contents and cafestol/kahweol ratio. using the d ...201829323254
effects of alternative cropping systems on globe artichoke qualitative traits.traditionally, globe artichoke cultivation in the mediterranean basin is based on monoculture and on use of high amounts of nitrogen fertiliser. this raises issues regarding its compatibility with sustainable agriculture. we studied the effect of one typical conventional (conv) and two alternative cropping systems [globe artichoke in sequence with french bean (ncv1), or in biannual rotation (ncv2) with cauliflower and with a leguminous cover crop in inter-row spaces] on yield, polyphenol and min ...201828722756
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