Publications

purification, crystallization and preliminary x-ray crystallographic analysis of glycosyltransferase-1 from bacillus cereus.	glycosyltransferases (gts), which are distributed widely in various organisms, including bacteria, fungi, plants and animals, play a role in synthesizing biological compounds. glycosyltransferase-1 from bacillus cereus (bcgt-1), which is capable of transferring glucose to small molecules such as kaempferol and quercetin, has been identified as a member of the family 1 glycosyltransferases which utilize uridine diphosphate glucose (udp-glucose) as the sugar donor. bcgt-1 (molecular mass 45.5 kda) ...	2014	25195897
enhancement of the immune response and protection against vibrio parahaemolyticus by indigenous probiotic bacillus strains in mud crab (scylla paramamosain).	in a previous study, bacterial communities of the intestine in three populations of crabs (wild crabs, pond-raised healthy crabs and diseased crabs) were probed by culture-independent methods. in this study, we examined the intestinal communities of the crabs by bacterial cultivation with a variety of media. a total of 135 bacterial strains were isolated from three populations of mud crabs. the strains were screened for antagonistic activity against vibrio parahaemolyticus using an agar spot ass ...	2014	25193866
characterization of three bacillus cereus strains involved in a major outbreak of food poisoning after consumption of fermented black beans (douchi) in yunan, china.	three bacillus cereus strains isolated from an outbreak of food poisoning caused by the consumption of fermented black beans (douchi) containing b. cereus is described. the outbreak involved 139 persons who had nausea, vomiting, and diarrhea. the strains were isolated from vomit and the unprepared douchi. two of the strains produced the emetic toxin cereulide, as evidenced by polymerase chain reaction analysis for the presence of the nonribosomal synthetase cluster responsible for the synthesis ...	2014	25188780
analgesic, antibacterial and central nervous system depressant activities of albizia procera leaves.	to ascertain analgesic, antibacterial and central nervous system (cns) depressant activities of ethyl acetate, dichloromethane and carbon tetrachloride fractions of methanol extract of albizia procera (a. procera) leaves.	2014	25182551
cspa encodes a major cold shock protein in himalayan psychrotolerant pseudomonas strains.	the major cold-shock protein (cspa) encoding gene cspa were detected in three himalayan psychrotrophic pseudomonad strains, by pcr amplification. partial sequencing of three pseudomonas strains cspa gene and blast search confirmed the high similarity with putative bacterial cspa gene and bacterial cspa protein. bioinformatics analysis of these partial cspa amino acid sequences showed presence of putative conserved region for dna/rna-binding motifs rnp-1 and rnp-2. protein homologies of all three ...	2014	25172452
thermal inactivation parameters of spores from different phylogenetic groups of bacillus cereus.	the thermal inactivation kinetics of spores from 39 bacillus cereus strains belonging to six different phylogenetic groups (group ii to vii) was studied. fresh spore suspensions in glass capillaries were heated in an oil bath at three or more different temperatures for five different times. survival curves and thermal death curves were established and the kinetic parameters dt (decimal reduction time at temperature t) and z (temperature dependence of dt) were derived by linear regression. most s ...	2014	25171111
biocontrol of aspergillus species on peanut kernels by antifungal diketopiperazine producing bacillus cereus associated with entomopathogenic nematode.	the rhabditid entomopathogenic nematode associated bacillus cereus and the antifungal compounds produced by this bacterium were evaluated for their activity in reducing postharvest decay of peanut kernels caused by aspergillus species in in vitro and in vivo tests. the results showed that b. cereus had a significant effect on biocontrol effectiveness in in vitro and in vivo conditions. the antifungal compounds produced by the b. cereus were purified using silica gel column chromatography and the ...	2014	25157831
cobalt(ii) complexes with the antimicrobial drug enrofloxacin: structure, antimicrobial activity, dna- and albumin-binding.	the cobalt(ii) complexes with the quinolone antimicrobial agent enrofloxacin (herx) in the presence of the nitrogen-donor heterocyclic ligands pyridine (py), 2,2'-bipyridylamine (bipyam), 1,10-phenanthroline (phen), 2,2'-bipyridine (bipy) or the oxygen-donor ligand n,n-dimethylformamide (dmf) were synthesized and characterized. the crystal structures of complexes [co(erx)2(py)2]·meoh·6h2o and [co(erx)2(bipyam)]·4.5meoh·1.25h2o were determined by x-ray crystallography. the deprotonated enrofloxac ...	2014	25151581
collagen-like glycoprotein bcls is involved in the formation of filamentous structures of the lysinibacillus sphaericus exosporium.	lysinibacillus sphaericus produces mosquitocidal binary toxins (bin toxins) deposited within a balloon-like exosporium during sporulation. unlike bacillus cereus group strains, the exosporium of l. sphaericus is usually devoid of the hair-like nap, an external filamentous structure formed by a collagen-like protein, bcla. in this study, a new collagen-like exosporium protein encoded by bsph_0411 (bcls) from l. sphaericus c3-41 was characterized. thin-section electron microscopy revealed that del ...	2014	25149519
biodegradation of hard keratins by two bacillus strains.	extensive quantities of keratinic by-products are disposed annually by animal-processing industry, causing a mounting ecological problem due to extreme resilience of these materials to enzymatic breakdown. there is a growing trend to apply cheap and environment-friendly methods to recycle keratinic wastes. soil bacteria of profound keratinolytic potential, especially spore-forming rods from the genus bacillus, play a significant role in keratinase-mediated biodegradation of keratins, therefore c ...	2014	25147672
the antibacterial activity of cassia fistula organic extracts.	cassia fistula, is a flowering plant and a member of fabaceae family. its leaves are compound of 4 - 8 pairs of opposite leaflets. there are many cassia species around the world which are used in herbal medicine.	2014	25147664
first synthesis of bacillus cereus ch hf-ps cell wall trisaccharide repeating unit.	the first total synthesis of ch hf-ps, a cell wall trisaccharide repeating unit of b. cereus, is reported. the synthetic trisaccharide is appended with an aminopropyl linker at the reducing end to allow for conjugation to proteins and microarrays. the convergent synthesis involves transformation of d-mannose into an orthogonally protected rare aat sugar building block, two consecutive α-stereoselective glycosylations, β-selective attachment of the linker by solvent participation, and amide bond ...	2014	25101800
the genomes, proteomes, and structures of three novel phages that infect the bacillus cereus group and carry putative virulence factors.	this article reports the results of studying three novel bacteriophages, jl, shanette, and basilisk, which infect the pathogen bacillus cereus and carry genes that may contribute to its pathogenesis. we analyzed host range and superinfection ability, mapped their genomes, and characterized phage structure by mass spectrometry and transmission electron microscopy (tem). the jl and shanette genomes were 96% similar and contained 217 open reading frames (orfs) and 220 orfs, respectively, while basi ...	2014	25100842
thiophene acetylenes and furanosesquiterpenes from xanthopappus subacaulis and their antibacterial activities.	in a search for naturally occurring antibacterial compounds in medicinal plants, six hitherto unknown thiophene acetylenes, named 10,11-threo-xanthopappin d, 10,11-erythro-xanthopappin d, 10,11-cis-xanthopappin b, 5-(but-4-chloro-3-hydroxy-1-ynyl)-2-(z)-pent-3-ene-1-ynylthiophene, 5-(but-4-chloro-3-hydroxy-1-ynyl)-2-(e)-pent-3-ene-1-ynylthiophene, 5-(but-3,4-dihydroxy-1-ynyl)-2-(z)-pent-3-ene-1-ynylthiophene and two furanosesquiterpenes, as well as fifteen known compounds, were isolated from xan ...	2014	25096755
sub-emetic toxicity of bacillus cereus toxin cereulide on cultured human enterocyte-like caco-2 cells.	cereulide (cer) intoxication occurs at relatively high doses of 8 µg/kg body weight. recent research demonstrated a wide prevalence of low concentrations of cer in rice and pasta dishes. however, the impact of exposure to low doses of cer has not been studied before. in this research, we investigated the effect of low concentrations of cer on the behavior of intestinal cells using the caco-2 cell line. the mtt (mitochondrial 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) and the s ...	2014	25093386
characterization of lipoteichoic acid structures from three probiotic bacillus strains: involvement of d-alanine in their biological activity.	probiotics represent a potential strategy to influence the host's immune system thereby modulating immune response. lipoteichoic acid (lta) is a major immune-stimulating component of gram-positive cell envelopes. this amphiphilic polymer, anchored in the cytoplasmic membrane by means of its glycolipid component, typically consists of a poly (glycerol-phosphate) chain with d-alanine and/or glycosyl substitutions. lta is known to stimulate macrophages in vitro, leading to secretion of inflammatory ...	2014	25090957
efficacy of gaseous chlorine dioxide in inactivating bacillus cereus spores attached to and in a biofilm on stainless steel.	we evaluated the lethal activity of gaseous chlorine dioxide (clo2) against bacillus cereus spores attached to and in biofilm formed on a stainless steel surface. aqueous clo2 was prepared by mixing sulfuric acid (5% w/v) with sodium chlorite (10mg/ml), and gaseous clo2 was produced by vaporization of aqueous clo2 in an air-tight container. the concentration of gaseous clo2 in the air within the container increased rapidly at first but gradually decreased over time. the lethality of gaseous clo2 ...	2014	25090607
cytotoxic and antibacterial quinone sesquiterpenes from a myrothecium fungus.	six new quinone sesquiterpenes, myrothecols a-f (1-6), and hymenopsin b (7) were isolated from cultures of myrothecium sp. sc0265 by bioassay-guided fractionation. their structures were elucidated on the basis of 1d and 2d nmr and ms data. the absolute configurations of the new compounds were assigned by cd/tddft calculations, and that of and hymenopsin b was confirmed by x-ray diffraction analysis. compounds 1-5 and hymenopsin demonstrated cytotoxic activity against human carcinoma a549, hela, ...	2014	25089733
quorum sensing in bacillus thuringiensis is required for completion of a full infectious cycle in the insect.	bacterial cell-cell communication or quorum sensing (qs) is a biological process commonly described as allowing bacteria belonging to a same pherotype to coordinate gene expression to cell density. in gram-positive bacteria, cell-cell communication mainly relies on cytoplasmic sensors regulated by secreted and re-imported signaling peptides. the bacillus quorum sensors rap, nprr, and plcr were previously identified as the first members of a new protein family called rnpp. except for the rap prot ...	2014	25089349
a laboratory case study of efficient polyhydoxyalkonates production by bacillus cereus, a contaminant in saccharophagus degradans atcc 43961 in minimal sea salt media.	a contaminating bacterium growing along with the stock culture of saccharophagus degradans atcc 43961 (sde 2-40) on marine agar plate was isolated and investigated for its ability to produce polyhydoxyalkonates (pha). preliminary screening by sudan black b and nile blue a staining indicated positive characteristic of the isolate to produce pha. the isolate was able to grow and produce pha in minimal sea salt medium broth. pha quantification studies with gas chromatographic analyses of the dry ce ...	2014	25085545
potential of kalopanax septemlobus leaf extract in synthesis of silver nanoparticles for selective inhibition of specific bacterial strain in mixed culture.	silver nanoparticles (agnps) were synthesised using kalopanax septemlobus plant leaf extracts. uv-visible spectrophotometric, fourier-transform infrared, electron dispersive x-ray spectroscopy, scanning electron microscopy (sem) and transmission electron microscopy (tem) analyses confirmed synthesis of agnps. tem micrographs revealed presence of well-dispersed agnps predominantly of small size and different shapes with an average particle size of 30.8 nm. antimicrobial susceptibility tests of ag ...	2014	25085530
incidence, diversity and characteristics of spores of psychrotolerant spore formers in various repfeds produced in belgium.	the major objectives of this study were to determine the incidence of psychrotolerant spore formers from repfeds marketed in belgium, and their diversity and characteristics. spore formers in general were found as spores on 38.3% of the food samples and in 85% food products types evaluated. 76% of the food samples containing spore formers had spores before enrichment. a total of 86 spore formers were isolated from the samples. 28 of 86 bacterial spore formers (32.6%) were capable of vegetative g ...	2014	25084675
inactivation of bacillus spores inoculated in milk by ultra high pressure homogenization.	ultra high-pressure homogenization treatments at 300 mpa with inlet temperatures (ti) of 55, 65, 75 and 85 °c were applied to commercial ultra high temperature treated whole milk inoculated with bacillus cereus, bacillus licheniformis, bacillus sporothermodurans, bacillus coagulans, geobacillus stearothermophilus and bacillus subtilis spores in order to evaluate the inactivation level achieved. ultra high-pressure homogenization conditions at 300 mpa with ti = 75 and 85 °c were capable of a spor ...	2014	25084664
secdf as part of the sec-translocase facilitates efficient secretion of bacillus cereus toxins and cell wall-associated proteins.	the aim of this study was to explore the role of secdf in protein secretion in bacillus cereus atcc 14579 by in-depth characterization of a markerless secdf knock out mutant. deletion of secdf resulted in pleiotropic effects characterized by a moderately slower growth rate, aberrant cell morphology, enhanced susceptibility to xenobiotics, reduced virulence and motility. most toxins, including food poisoning-associated enterotoxins nhe, hbl, and cytotoxin k, as well as phospholipase c were less a ...	2014	25083861
chemometric approach to prediction of antibacterial agent production by streptomyces hygroscopicus.	the nutritional requirements for antimicrobial agent production using streptomyces hygroscopicus were analyzed in shake flask experiments. antimicrobial activity was tested against staphylococcus aureus and bacillus cereus. the mathematical models have been generated with relative high complexity in order to give an adequate fit to the data. all the results suggest a high dependence of produced antimicrobial agent quantities on the amount of carbon, nitrogen, and phosphorus in cultivation medium ...	2014	25082769
[effects of inoculating plant growth-promoting rhizobacteria on the biological characteristics of walnut (juglans regia) rhizosphere soil under drought condition].	effects of four plant growth-promoting rhizobacteria (pgpr) , namely pseudomonas sp. yt3, bacillus subtilis dz1, b. cereus l90 and b. fusiformis l13 on the biological characteristics of walnut (juglans regia) rhizosphere soil under drought stress were investigated. results showed that drought stress had little effect on available nutrients of walnut rhizosphere soil, but significantly decreased the activity of organic carbon by 18.4% and increased the ph from 7.34 to 7.79. under drought stress c ...	2014	25129951
volatile constituents of dianthus rupicola biv. from sicily: activity against microorganisms affecting cellulosic objects.	dianthus rupicola biv. (cliffs carnation) is a camephytic, suffruticous, perennial plant growing up to 40 cm high. the plant is widespread in sicily and neighbouring islands (egadi, lampedusa, lipari) and in some areas of southern italy. gc and gc-ms analyses of the essential oil distilled from the flowers showed the presence of 66 components. its composition is characterised by the high content of thymol and carvacrol derivatives. a good antibacterial activity against bacillus cereus and bacill ...	2014	25115728
modular genetic architecture of the toxigenic plasmid pis56-63 harboring cry1ab21 in bacillus thuringiensis subsp. thuringiensis strain is5056.	bacillus thuringiensis subsp. thuringiensis is5056, a strain highly toxic to trichoplusia ni larvae, produces the newly described cry1ab21 delta-endotoxin encoded by a gene located in the 63.8 kb pis56-63 plasmid. in this report we present the structure and functional similarity of this plasmid to other b. thuringiensis large toxigenic plasmids with particular interest focused on its modular architecture. the 61 open reading frames (orfs) of the plasmid made four functional modules: (i) m1-mic, ...	2014	25115108
comparison of identification systems for psychrotrophic bacteria isolated from raw bovine milk.	psychrotrophic bacteria in raw milk produce heat-resistant extracellular proteases, resulting in spoilage and shelf-life reduction of ultrahigh temperature treated milk and milk products. controlling of these spoilage microbes requires rapid and reliable identification systems for screening of raw milk. this study aimed to compare commercial bacterial identification systems with a genetic method (considered as the 'gold standard' method) for the identification of heat-resistant protease producin ...	2014	25113043
antibacterial tetraoxygenated xanthones from the immature fruits of garcinia cowa.	a phytochemical investigation of the acetone extract from the immature fruits of garcinia cowa led to the isolation of two novel tetraoxygenated xanthones, garcicowanones a (1) and b (2), together with eight known tetraoxygeanted xanthones. their structures were determined by spectroscopic analysis. all isolated compounds were evaluated for their antibacterial activity against bacillus cereus tistr 688, bacillus subtilis tistr 008, micrococcus luteus tistr 884, staphylococcus aureus tistr 1466, ...	2014	25110196
new thiophene-1,2,4-triazole-5(3)-ones: highly bioactive thiosemicarbazides, structures of schiff bases and triazole-thiols.	key compound 2-(4-amino-5-oxo-3-(thiophene-2-ylmethyl)-4,5-dihydro-1,2,4-tiazole-1-yl) acetohydrazide (3) was synthesized by reacting hydrazine hydrate with ethyl-2-(4-amino-5-oxo-3-(thiophene-2-ylmethyl)-4,5-dihydro-1,2,4-tiazole-1yl)acetate (2), obtained in basic media from 4-amino-5-(thiophene-2-ylmethyl)-2h-1,2,4-triazole-3(4h)-one (1). compound 3 was converted to thiosemicarbazide derivatives (4a-d) and schiff base derivatives 6a-e and 7a-e. the treatment of compound 4 with naoh gave 4-amin ...	2014	25063946
impact of a probiotic bacillus cereus strain on the jejunal epithelial barrier and on the nkg2d expressing immune cells during the weaning phase of piglets.	in a feeding experiment, the probiotic bacillus cereus var. toyoi was fed to sows and piglets in order to test whether it influences the stress response of enterocytes, thereby causing intestinal immune activation, possibly accompanied by an impairment of the epithelial integrity. the impact of b. cereus on the piglets' intestinal enterocytes (ec) and on the communicating intraepithelial lymphocytes (iel) was investigated during the weaning phase where significant immunological changes might be ...	2014	25063224
regulation of cry gene expression in bacillus thuringiensis.	bacillus thuringiensis differs from the closely related bacillus cereus group species by its ability to produce crystalline inclusions. the production of these crystals mainly results from the expression of the cry genes, from the stability of their transcripts and from the synthesis, accumulation and crystallization of large amounts of insecticidal cry proteins. this process normally coincides with sporulation and is regulated by various factors operating at the transcriptional, post-transcript ...	2014	25055802
inactivation of bacteria via photosensitization of vitamin k3 by uv-a light.	this study investigated inactivation of bacteria with ultraviolet light a irradiation in combination with vitamin k3 as a photosensitizer. six bacteria including bacillus cereus, pseudomonas aeruginosa, staphylococcus aureus, staphylococcus epidermidis, klebsiella pneumoniae, and escherichia coli suspended in vitamin k3 aqueous solution were exposed to ultraviolet light a. five of six bacteria, with the exception of pseudomonas aeruginosa, were reduced by eight logs with 1600 μm of vitamin k3 an ...	2014	25053393
green synthesis of silver nanoparticles using delphinium denudatum root extract exhibits antibacterial and mosquito larvicidal activities.	green synthesis of silver nanoparticles (agnps) using aqueous root extract of delphinium denudatum (dd) by reduction of ag(+) ions from silver nitrate solution has been investigated. the synthesized ddagnps were characterized by using uv-vis spectroscopy, x-ray diffraction (xrd), field emission scanning electron microscope (fesem) and fourier transform infrared spectroscopy (ftir). the prepared ddagnps showed maximum absorbance at 416nm and particles were polydispersed in nature, spherical in sh ...	2014	24632157
a fullerene colloidal suspension stimulates the growth and denitrification ability of wastewater treatment sludge-derived bacteria.	fullerene (c60) is a nanoparticle that has been widely studied and applied in numerous commodities. however, there are concerns regarding its potential negative impact on the environment. a fullerene colloidal suspension (nc60) is known for its property of selectively inhibiting the growth of microorganisms. in this study, using denaturing gradient gel electrophoresis fingerprinting technology, we found that fullerene altered the structure of a sludge-derived microbial community. specifically, t ...	2014	24630252
diversity of pulsed-field gel electrophoresis patterns of cereulide-producing isolates of bacillus cereus and bacillus weihenstephanensis.	bacillus cereus is an important foodborne pathogen causing diarrhoea, emesis and in, rare cases, lethal poisonings. the emetic syndrome is caused by cereulide, a heat-stable toxin. originally considered as a rather homogenous group, the emetic strains have since been shown to display some diversity, including the existence of two clusters of mesophilic b. cereus and psychrotolerant b. weihenstephanensis. using pulsed-field gel electrophoresis (pfge) analysis, this research aimed to better unders ...	2014	24627989
association between neutropenia and brain abscess due to bacillus cereus bacteremia in patients with hematological malignancies.		2014	24625329
comparative proteomic analysis of experimental evolution of the bacillus cereus-ketogulonicigenium vulgare co-culture.	the microbial co-culture system composing of ketogulonicigenium vulgare and bacillus cereus was widely adopted in industry for the production of 2-keto-gulonic acid (2-kga), the precursor of vitamin c. we found serial subcultivation of the co-culture could enhance the yield of 2-kga by 16% in comparison to that of the ancestral co-culture. to elucidate the evolutionary dynamics and interaction mechanisms of the two microbes, we performed itraq-based quantitative proteomic analyses of the pure cu ...	2014	24619085
isolation and characterization of chitinase-producing bacillus and paenibacillus strains from salted and fermented shrimp, acetes japonicus.	chitinases catalyze the conversion of chitin and are produced by a wide range of bacteria. the biological applications of these enzymes have been exploited in food and pharmaceutical industries. we isolated 2 halophilic chitinase-producing novel strains of bacteria-sch-1 and sch-2 from saeu-jeot, a traditional korean salted and fermented food made with shrimp (acetes japonicus). the isolated strains- sch-1 and sch-2 were gram-positive, rod-shaped, endospore-forming facultative anaerobes, with st ...	2014	24611959
quantification of the impact of single and multiple mild stresses on outgrowth heterogeneity of bacillus cereus spores.	outgrowth heterogeneity of bacterial spore populations complicates both prediction and efficient control of spore outgrowth. in this study, the impact of mild preservation stresses on outgrowth of bacillus cereus atcc 14579 spores was quantified during the first stages of outgrowth. heterogeneity in outgrowth of heat-treated (90°c for 10 min) and non-heat-treated germinated single spores to the maximum micro-colony stage of 256 cells was assessed by direct imaging on anopore strips, placed on bh ...	2014	24607860
crystal and solution structure analysis of fhud2 from staphylococcus aureus in multiple unliganded conformations and bound to ferrioxamine-b.	iron acquisition is a central process for virtually all organisms. in staphylococcus aureus, fhud2 is a lipoprotein that is a high-affinity receptor for iron-bound hydroxamate siderophores. in this study, fhud2 was crystallized bound to ferrioxamine-b (fxb), and also in its ligand-free state; the latter structures are the first for hydroxamate-binding receptors within this protein family. the structure of the fhud2-fxb conformation shows that residues w197 and r199 from the c-terminal domain don ...	2014	24606332
de-hairing protease production by an isolated bacillus cereus strain at under solid-state fermentation using cow dung: biosynthesis and properties.	agro-industrial residues and cow dung were used as the substrate for the production of alkaline protease by bacillus cereus strain at. the bacterial strain bacillus cereus strain at produced a high level of protease using cow dung substrate (4813 ± 62 u g(-1)). physiological fermentation factors such as the incubation time (72 h), the ph (9), the moisture content (120%), and the inoculum level (6%) played a vital role in the enzyme bioprocess. the enzyme production improved with the supplementat ...	2014	24596497
characterization and genome analysis of the bacillus cereus-infecting bacteriophages bps10c and bps13.	due to the emergence of antibiotic-resistant strains, bacteriophages are considered to be an alternative approach for the control of pathogens. in this study, the bacteriophages bps10c and bps13 were isolated and characterized to investigate their ability to control food-borne pathogenic bacillus cereus. phage bps13 exhibited slightly higher host lysis activity compared with phage bps10c. in addition, phage bps13 exhibited greater stability under various ph and temperature conditions. to extend ...	2014	24595561
soy whey based medium for optimized phytase activity in saccharomyces cerevisiae mtcc 5421 and α-d-galactosidase and antibacterial activities in lactobacillus plantarum mtcc 5422 by response surface methodology.	soy whey based growth medium for yeast enabled the native isolate of saccharomyces cerevisiae mtcc 5421 to produce 198 u/ml of phytase activity against that of 135 u/ml in potato dextrose broth. in the same medium for lactic acid bacteria, the isolate of lactobacillus plantarum mtcc 5422 was able to elaborate α-d-galactosidase activity of 10.6 u/ml in comparison to 6 u/ml in lactobacillus mrs broth. using this formulated medium, a central composite experimental design based on 5 variables of 3 f ...	2014	24587527
seasonal trend and clinical presentation of bacillus cereus bloodstream infection: association with summer and indwelling catheter.	bacillus cereus, an opportunistic pathogen, can cause fatal infection. however, b. cereus bloodstream infections (bsis) have not been well characterised. from 2008 to 2013, b. cereus isolates from all of the specimens and patients with b. cereus bsis were identified. environmental samples were collected to detect b. cereus contamination. we also characterised the clinical presentation of b. cereus bsi through analyses of risk factors for bsi and mortality. a total of 217 clinical b. cereus isola ...	2014	24584718
novel inhibitor of bacterial sphingomyelinase, smy-540, developed based on three-dimensional structure analysis.	bacterial sphingomyelinase (smase) is thought to play a crucial role in bacterial evasion of the immune response during the early stages of infections.	2014	23488740
bacillus cereus bacteremia and multiple brain abscesses during acute lymphoblastic leukemia induction therapy.	bacillus cereus can cause serious infections in immunosuppressed patients. this population may be susceptible to b. cereus pneumonia, bacteremia, cellulitis, and rarely cerebral abscess. here we report an 8-year-old boy undergoing induction therapy for acute lymphoblastic leukemia who developed multifocal b. cereus cerebral abscesses, highlighting the propensity for b. cereus to develop cerebral abscesses. a review of the literature over the past 25 years identified another 11 cases (3 children ...	2014	23619116
identification of cultivable bacteria in the intestinal tract of bactrocera dorsalis from three different populations and determination of their attractive potential.	this study aimed to identify the cultivable bacteria inhabiting the intestinal tract of adult oriental fruit flies (bactrocera dorsalis) from laboratory-reared, laboratory sterile sugar-reared, and field-collected populations, and to evaluate the attractiveness of the metabolites produced by the above bacteria to their hosts.	2014	23696217
microbial toxicity of ethanolamines--multiwalled carbon nanotubes.	in the present study, antimicrobial activities of multiwalled carbon nanotubes (mwcnts) functionalized with ethanolamine (ea) groups were investigated. therefore, mwcnt were first functionalized with mono-, di-, and triethanolamine (mea, dea, and tea) under microwave technique. development of functional groups on the mwcnt surface was confirmed by fourier transform infrared and thermogravimetric analysis. morphological variation was investigated by transmission electron microscopy. then, antimic ...	2014	23784887
rna-seq analysis of antibiotic-producing bacillus subtilis sc-8 in response to signal peptide papr of bacillus cereus.	bacillus subtilis sc-8 produces an antibiotic that has narrow antagonistic activity against bacteria in the bacillus cereus group. in b. cereus group bacteria, peptide-activating plcr (papr) plays a significant role in regulating the transcription of virulence factors. when b. subtilis sc-8 and b. cereus are co-cultured, papr is assumed to stimulate antibiotic production by b. subtilis sc-8. to better understand the effect of papr on this interspecies interaction, the global transcriptome profil ...	2014	24104687
cyclo(l-pro-d-arg): a new antibacterial and antitumour diketopiperazine from bacillus cereus associated with a rhabditid entomopathogenic.	in continuation of our search for new antimicrobial secondary metabolites from bacillus cereus associated with rhabditid entomopathogenic nematode, a new microbial diketopiperazine, cyclo(l-pro-d-arg), was isolated from the ethyl acetate extract of fermented modified nutrient broth. the chemical structures of the isolated compounds were identified based on their 1d, 2d nmr and high-resolution electrospray ionisation-mass spectroscopy data. antibacterial activity of the compound was determined by ...	2014	24097052
functional role of the mrpa- and mrpd-homologous protein subunits in enzyme complexes evolutionary related to respiratory chain complex i.	nadh:quinone oxidoreductase or complex i is a large membrane bound enzyme complex that has evolved from the combination of smaller functional building blocks. intermediate size enzyme complexes exist in nature that comprise some, but not all of the protein subunits in full size 14-subunit complex i. the membrane spanning complex i subunits nuol, nuom and nuon are homologous to each other and to two proteins from one particular class of na(+)/h(+) antiporters, denoted mrpa and mrpd. in complex i, ...	2014	24095649
computational analysis of trappc9: candidate gene for autosomal recessive non-syndromic mental retardation.	mental retardation (mr)/ intellectual disability (id) is a neuro-developmental disorder characterized by a low intellectual quotient (iq) and deficits in adaptive behavior related to everyday life tasks such as delayed language acquisition, social skills or self-help skills with onset before age 18. to date, a few genes (prss12, crbn, cc2d1a, grik2, tusc3, trappc9, tecr, st3gal3, med23, man1b1, nsun1) for autosomal-recessive forms of non syndromic mr (ns-armr) have been identified and establishe ...	2014	24040793
chemical composition and bioactivity of essential oils of hypericum helianthemoides, hypericum perforatum and hypericum scabrum.	a number hypericum species are well known for their therapeutic efficacy and use in traditional medicine. the various species of hypericum have been traditionally used for the treatment of wounds, eczema, burns, trauma, rheumatism, neuralgia, gastroenteritis, ulcers, hysteria, bedwetting and depression.	2014	24073965
potential cellular targets and antibacterial efficacy of atmospheric pressure non-thermal plasma.	atmospheric pressure non-thermal plasma (apntp) has been gaining increasing interest as a new alternative antibacterial approach. although this approach has demonstrated promising antibacterial activity, its exact mechanism of action remains unclear. mechanistic elucidation of the antimicrobial activity will facilitate development and rational optimisation of this approach for potential medical applications. in this study, the antibacterial efficacy of an in-house-built apntp jet was evaluated a ...	2014	24139886
fusarial wilt control and growth promotion of pigeon pea through bioactive metabolites produced by two plant growth promoting rhizobacteria.	the bioactive metabolites produced by two plant growth promoting rhizobacteria strains, a pseudomonas aeruginosa strain rrlj 04 and a bacillus cereus strain bs 03, which showed growth promotion and disease control in pigeon pea against fusarium udum, were isolated and screened for their efficacy to control fusarial wilt of pigeon pea under gnotobiotic and nursery condition. bioactive metabolites viz., bm 1 and bm 2 from rrlj 04 and bm 3 from bs 03 also showed in vitro antibiosis against f. udum. ...	2014	24154979
development of new active packaging film made from a soluble soybean polysaccharide incorporated zataria multiflora boiss and mentha pulegium essential oils.	an active edible film from soluble soybean polysaccharide (ssps) incorporated with different concentrations of zataria multiflora boiss (zeo) and mentha pulegium (meo) essential oils was developed, and the film's optical, wettability, thermal, total phenol and antioxidant characteristics were investigated, along with their antimicrobial effectiveness against staphylococcus aureus, bacillus cereus, escherichia coli o157:h7, pseudomonas aeruginosa and salmonella typhimurium. the film's colour beca ...	2014	24176389
bacillus cereus enterotoxins act as major virulence factors and exhibit distinct cytotoxicity to different human cell lines.	a comparative analysis on the relevance of the bacillus cereus enterotoxins nhe (nonhemolytic enterotoxin), hbl (haemolysin bl) and cytk (cytotoxin k) was accomplished, concerning their toxic activity towards different target cell lines. overall, among the components secreted by the reference strains for nhe and hbl, the enterotoxin complexes accounted for over 90% of the total toxicity. vero and primary endothelial cells (huvec) were highly susceptible to nhe, whereas hep-g2, vero and a549 reac ...	2014	24211313
prevalence, pfge typing, and antibiotic resistance of bacillus cereus group isolated from food in morocco.	this article reports the prevalence and antibiotic resistance of the bacillus cereus group isolated from different foods (milk and dairy products, spices, and rice salad) in morocco. in total, 402 different food samples collected from 2008 to 2010 were analyzed by microbiological methods to isolate b. cereus. the strains were subjected to a polymerase chain reaction test in order to verify whether they belonged to the b. cereus group. sixty-four of all isolates (15.9%) were found to be positive. ...	2014	24206436
complete ¹h, ¹⁵n, and ¹³c resonance assignments of bacillus cereus metallo-β-lactamase and its complex with the inhibitor r-thiomandelic acid.	β-lactamases inactivate β-lactam antibiotics by hydrolysis of their endocyclic β-lactam bond and are a major cause of antibiotic resistance in pathogenic bacteria. the zinc dependent metallo-β-lactamase enzymes are of particular concern since they are located on highly transmissible plasmids and have a broad spectrum of activity against almost all β-lactam antibiotics. we present here essentially complete (>96%) backbone and sidechain sequence-specific nmr resonance assignments for the bacillus ...	2014	23838816
comparative studies to assess bacterial communities on the clover phylloplane using mlst, dgge and t-rflp.	denaturing gradient gel electrophoresis (dgge) and terminal restriction fragment length polymorphism (t-rflp) were used to characterise the changes that occurred in bacillus cereus group strains present in the phylloplane of clover trifolium hybridum over 4 months. these strains had previously been analysed by multiple locus sequence typing (mlst). dgge displayed many equally intense bands which indicated many equally abundant ribotypes. the bacterial community composition was variable and the l ...	2014	23861044
antimicrobial activity of amazon astrocaryum aculeatum extracts and its association to oxidative metabolism.	several compounds present in fruits as polyphenols are able to kill or inhibit the growth of microorganisms. these proprieties are relevant mainly in tropical areas, as amazonian region where infectious are highly prevalent. therefore, this study investigated the antimicrobial activity of tucumã amazonian fruit against 37 microorganisms. the potential role of oxidative metabolism imbalance was also studied as causal mechanism of antimicrobial activity. the results showed antibacterial effect of ...	2014	23870852
community composition and cellulase activity of cellulolytic bacteria from forest soils planted with broad-leaved deciduous and evergreen trees.	cellulolytic bacteria in forest soil provide carbon sources to improve the soil fertility and sustain the nutrient balance of the forest ecological system through the decomposition of cellulosic remains. these bacteria can also be utilized for the biological conversion of biomass into renewable biofuels. in this study, the community compositions and activities of cellulolytic bacteria in the soils of forests planted with broad-leaved deciduous (chang qing garden, cqg) and broad-leaved evergreen ...	2014	23893311
the diversity and antimicrobial activity of preussia sp. endophytes isolated from australian dry rainforests.	limited knowledge currently exists regarding species diversity and antimicrobial activity of endophytic isolates of preussia within australia. this report describes endophytic preussia species that were identified through molecular analysis of the internal transcribed spacer region. screening for antimicrobial secondary metabolites was determined by testing crude ethyl acetate (etoac) extracts derived from fungal mycelia against a panel of atcc type strains which included bacillus cereus, escher ...	2014	23975673
identification and characterization of a novel marine bacillus cereus for mosquito control.	entomopathogenic bacteria to control mosquitoes are a promising environmentally friendly alternative to synthetic pesticides. in the present study, a novel mosquitocidal bacterium was isolated from marine soil collected from east coastal areas at pondicherry (india). 16s rrna gene sequence alignment depicted that this isolate belonged to bacillus cereus vcrc-b520 (ncbi: kc-119192). biochemical studies on bacterial growth, biomass, and toxin production have revealed that this strain could possibl ...	2014	24192866
enhanced antimicrobial activity of peptide-cocktails against common bacterial contaminants of ex vivo stored platelets.	bacterial contamination of blood components such as ex vivo-stored platelets is a major safety risk in transfusion medicine. we have recently shown that synthetic antimicrobial peptides named pd1-pd4 derived from the thrombin-induced human platelet-derived antimicrobial proteins, and repeats of arg-trp (rw1-rw5) demonstrate microbicidal activity against selected bacteria and viruses. in the present study, we selected pd3, pd4, rw2, rw3 and rw4 and evaluated each individual peptide and their vari ...	2014	23926880
optimization of fermentation conditions for green pigment production from bacillus cereus m¹ 16 (mtcc 5521) and its pharmacological application.	optimal culture conditions for the production of green pigment was investigated. the optimal culture condition for the production of an extracellular green pigment by growing bacillus cereus m(1) 16 (mtcc 5521) in a complex medium containing (g l(-1) ) peptone-4.0, beef extract-9.0, nacl-7.0, mgso4 .7h2 o-1.0 and kh2 po4 -5.0 was as follows ph-7.0 at 30°c for 72 h in a 5 l fermenter. aeration rate and agitator speed had no effect on the pigment production. thin layer chromatogram of the pigment ...	2014	23992047
[detection of toxigenic genes nhea, nheb and nhec in bacillus cereus strains isolated from powdered milk samples in costa rica].	powdered milk is a frequently consumed product that does not need to be kept under cold conditions. nevertheless, different microorganisms may contaminate it. powdered milk is a highly consumed product by costa rican population, and bacillus cereus is a potentially pathogenic bacteria associated to it, with the ability to develop toxins depending on the presence of the respective codifying genes. the aim of this study was to determine the presence of the toxigenic genes nhea, nheb and nhec from ...	2014	26137795
antimicrobial and cytotoxic activities from jatropha dioica roots.	the antimicrobial and cytotoxic activities of organic extracts obtained from roots of the medicinal plant jatropha dioica (euphorbiaceae) were investigated. in order to evaluate their antimicrobial activity, the organic extracts were tested against clinical isolates of the human pathogens bacillus cereus, escherichia coli, salmonella typhi, staphylococcus aureus, enterobacter aerogenes, enterobacter cloacae, salmonella typhimurium, cryptococcus neoformans, candida albicans, candida parapsilosis ...	2014	26031013
[screening of homoacetogen mixed culture converting h2/co2 to acetate].	homoacetogens are a group of microorganisms with application potential to produce chemicals and biofuels by the bioconversion of synthesis gas. in this study, we collected waste activated sludge samples to screen homoacetogens by hungate anaerobic technique, and studied the effect of ph on acetate and alcohol production from h2/co2 gas. the mixed culture contained clostridium ljungdahlii, lysinibacillus fusiformis and bacillus cereus. acetate concentration achieved 31.69 mmol/l when the initial ...	2014	26016379
[specific bacterial diversity in bats of different food guilds in southern sierra oaxaca, mexico].	specific bacterial diversity in bats of different food guilds in southern sierra oaxaca, mexico. bats have different ecologic roles in variable ecosystems that have been already described. they have been linked to several zoonoses, however little is known about the relationship between bat microbiota and their diet, and studies on the bacterial ecology of the microbiota in bats are limited. to contribute with the description of this important interaction between microbiota and host, the aim of t ...	2014	25720196
phenolic acids profile, antioxidant and antibacterial activity of chamomile, common yarrow and immortelle (asteraceae).	chamomile, common yarrow and immortelle ethanolic extracts were chemically analysed with respect to phenolics. twelve phenolic acids were separated and identified by hplc-dad and the presence of rosmarinic acid was additionally confirmed by lc-ms. five methods were applied for the evaluation of extracts' antioxidant properties (frap, dpph, abts, chelating activity, briggs-rauscher reaction), while the antibacterial activity was tested against some of the major food-borne pathogens (campylobacter ...	2014	25632475
quantitative and qualitative evaluation of bio-aerosols in surgery rooms and emergency department of an educational hospital.	bio-aerosols are a potential hazard in hospitals and are mostly produced by hospital staff, patients and visitors. bio-aerosols are solid or liquid particles pending in the air and they consist of aerosols accompanying micro-organisms or organic compounds of micro-organisms such as endotoxin, metabolite, toxin and other parts of organism. those are a potential hazard in hospitals and are mostly produced by hospital staff, patients and visitors.	2014	25632321
an outbreak of gastroenteritis linked to a buffet lunch served at a canberra restaurant.	in 2013, an outbreak of gastrointestinal illness occurred following a buffet lunch at a restaurant in canberra. an investigation was conducted to identify the cause of illness and to implement appropriate public health measures to prevent further disease. we conducted a retrospective cohort study via telephone interviews, using a structured questionnaire developed from the restaurant buffet menu. a case was defined as someone who ate the buffet lunch at the restaurant on the implicated date and ...	2014	25631587
introducing urtica dioica, a native plant of khuzestan, as an antibacterial medicinal plant.	urtica dioica is a flowering plant with long history of use in folk medicine and as a food source.	2014	25625045
characterization of a nanoscale s-layer protein based template for biomolecular patterning.	well organized template for biomolecular conjugation is the foundation for biosensing. most of the current devices are fabricated using lithographic patterning processes and self-assembly monolayer (sam) methods. however, the research toward developing a sub-10 nm patterned, self-regenerated template on various types of substrates is limited, mainly due to the limited functional groups of the building material. bacterial surface layer proteins (s-layer proteins) can self-assemble into ordered la ...	2014	25570568
hemorrhagic bullous lesions due to bacillus cereus in a cirrhotic patient.		2014	25566578
stem bark extract and fraction of persea americana (mill.) exhibits bactericidal activities against strains of bacillus cereus associated with food poisoning.	the study investigates the in vitro antibacterial potentials of stem bark extracts of persea americana on strains of bacillus cereus implicated in food poisoning. the crude stem bark extracts and butanolic fraction at a concentration of 25 mg/ml and 10 mg/ml, respectively, exhibited antibacterial activities against test isolates. the zones of inhibition exhibited by the crude extract and the fraction ranged between 10 mm and 26 mm, while the minimum inhibitory concentration values ranged between ...	2014	25558854
correction: genomic comparison of 93 bacillus phages reveals 12 clusters, 14 singletons and remarkable diversity.	the bacillus genus of firmicutes bacteria is ubiquitous in nature and includes one of the best characterized model organisms, b. subtilis, as well as medically significant human pathogens, the most notorious being b. anthracis and b. cereus. as the most abundant living entities on the planet, bacteriophages are known to heavily influence the ecology and evolution of their hosts, including providing virulence factors. thus, the identification and analysis of bacillus phages is critical to underst ...	2014	25547158
activity against microorganisms affecting cellulosic objects of the volatile constituents of leonotis nepetaefolia from nicaragua.	the chemical composition of the essential oil from aerial parts of l. nepetaefolia (l.) r. br. collected in nicaragua was evaluated by gc and gc-ms. the main components were (z)-phytol (22.8%), caryophyllene oxide (18.9%) and hexahydrofarnesylacetone (9.0%). the antibacterial activity against several gram + and gram - bacteria, including bacillus cereus and b. subtilis, both infesting historical cellulosic material, was also determined. b. cereus, b. subtilis and staphylococcus epidermis were th ...	2014	25532300
draft genome sequence of new bacillus cereus strain tsu1.	this paper reports the draft genome sequence of new bacillus cereus strain tsu1, isolated on an agar-cellulose plate. the draft genome sequence is 5.81 mb, revealing 5,673 coding sequences. it contains genes for cellulose-degradation and biosynthesis pathways of polyhydroxybutyrate (phb) and 8 rrna genes (5s, 16s, and 23s).	2014	25523768
quantitative analysis of cereulide toxin from bacillus cereus in rice and pasta using synthetic cereulide standard and 13c6-cereulide standard - a short validation study.	a single laboratory validation study of a rapid and sensitive quantitative method for the analysis of cereulide toxin produced by bacillus cereus using ultra high performance liquid chromatography-electrospray-tandem mass spectrometry is presented. the analysis of this cyclic peptide toxin was validated for pasta and rice samples using a newly presented synthetic cereulide peptide standard, together with 13c6-cereulide that previously have not been commercially available. the use of cereulide st ...	2014	25514093
[electron microscopy of the surfaces of bacillary spores].	the surface structures of the spores of bacillus. cereus, b. thuringiensis, and brevibacillus laterosporus were studied by transmission and scanning electron microscopy. platinum deposition and negative staining with uranyl acetate revealed appendages and exosporium in b. thuringiensis and b. cereus. the exosporium structure was visualized by negative staining and ultrathin sectioning. for staining the exosporium polysaccharide, alcian blue was used during fixation. the results obtained show the ...	2014	25509408
[purification of recombinant bacillus cereus resd-rese proteins expressed in escherichia coli strains].	recombinant e. coli strains expressing the bacillus cereus atcc 14579t resd and resegenes fused with the ubiquitin gene were constructed, and purification of the resd and rese proteins was performed. the approach used in the study allowed us to increase the protein yield of the electrophoretic homogeneous resd andrese proteins without denaturation steps up to 150 mg per gram of wet cell weight.	2014	25507776
detection of toxigenic bacillus cereus strains isolated from vegetables in mexico city.	bacillus cereus can cause diarrhea and emetic syndromes after ingestion of food contaminated with it. this ability is due to the production of enterotoxins by this microorganism, these being the hemolysin bl complex, which is involved in the diarrheal syndrome, and cereulide, which is responsible for the emetic syndrome. the detection of genes associated with the production of these toxins can predict the virulence of strains isolated from contaminated food. in this paper, we analyzed 100 sample ...	2014	25474064
surfactins in natto: the surfactin production capacity of the starter strains and the actual surfactin contents in the products.	surfactin-type lipopeptides are suspected of being implicated in the rare food poisonings caused by bacillus species outside the bacillus cereus cluster. in order to get information on surfactin levels in actual human foods, bacilli from three commercial samples of a japanese traditional bean product, natto, were isolated in order to clarify their potential to produce the suspect lipopeptides. the isolated bacilli were characterized as bacillus subtilis. they were β-hemolytic and gave a positive ...	2014	25474063
microbiological quality of seasoned roasted laver and potential hazard control in a real processing line.	microbiological quality of laver, one of the edible seaweeds, has not been reported in a real processing line. laver or supplements were collected from six manufacturers (a to f) to assess potential microbiological hazards and the critical control points in commercial processing lines. aerobic plate counts (apc), coliform counts, bacillus cereus, staphylococcus aureus, and vibrio parahaemolyticus were enumerated, and the presence of b. cereus, listeria monocytogenes, salmonella, s. aureus, and v ...	2014	25474052
effect of white mustard essential oil on the growth of foodborne pathogens and spoilage microorganisms and the effect of food components on its efficacy.	antimicrobial preservative compounds are added to foods to target specific pathogens and spoilage organisms. white mustard essential oil (wmeo) is an extract that contains 4-hydroxybenzyl isothiocyanate, a compound which has been demonstrated to have antimicrobial activity in limited studies. the objective of this research was to determine the in vitro antimicrobial activity of wmeo against gram-positive and gram-negative spoilage and pathogenic bacteria and determine the effect of food componen ...	2014	25474051
modeling of bacillus cereus growth in brown rice submitted to a combination of ultrasonication and slightly acidic electrolyzed water treatment.	the combined effects of ultrasonication and slight acidic electrolyzed water were investigated to improve the microbial safety of brown rice against bacillus cereus infection and to evaluate the growth kinetics of these bacteria during storage of untreated and treated rice at various temperatures (5, 10, 15, 20, 25, 30, and 35°c). the results indicate that this combination treatment was bactericidal against b. cereus, resulting in an approximately 3.29-log reduction. although b. cereus can be ef ...	2014	25474049
[purification of recombinant bacillus cereus resd-rese proteins expressed in escherichia coli strains].	recombinant e. coli strains expressing the bacillus cereus atcc 14579t resd and resegenes fused with the ubiquitin gene were constructed, and purification of the resd and rese proteins was performed. the approach used in the study allowed us to increase the protein yield of the electrophoretic homogeneous resd andrese proteins without denaturation steps up to 150 mg per gram of wet cell weight.	2014	25434178
kawasaki disease-specific molecules in the sera are linked to microbe-associated molecular patterns in the biofilms.	kawasaki disease (kd) is a systemic vasculitis of unknown etiology. the innate immune system is involved in its pathophysiology at the acute phase. we have recently established a novel murine model of kd coronary arteritis by oral administration of a synthetic microbe-associated molecular pattern (mamp). on the hypothesis that specific mamps exist in kd sera, we have searched them to identify kd-specific molecules and to assess the pathogenesis.	2014	25411968
comparison of 3 selective media for enumeration of bacillus cereus in several food matrixes.	in this study, we compared the inclusivity, exclusivity, recoverability, and selectivity of the 3 selective agars (mannitol yolk polymyxin b agar [mypa], polymyxin pyruvate egg yolk mannitol bromothymol blue agar [pemba], and brillance bacillus cereus agar [bbc agar]) for bacillus cereus (b. cereus) from pure culture and several food samples. bbc agar showed greater exclusivity and selectivity in pure culture and in foods with high background flora, respectively; however, all the tested media sh ...	2014	25399752
infrared decontamination of oregano: effects on bacillus cereus spores, water activity, color, and volatile compounds.	infrared (ir) heating, a novel technology for decontaminating oregano, was evaluated by investigating the reduction of inoculated bacillus cereus spores and the effect on water activity (a(w)), color, and headspace volatile compounds after exposure to ir treatment. conditioned oregano (a(w) 0.88) was ir-treated in a closed heating unit at 90 and 100 °c for holding times of 2 and 10 min, respectively. the most successful reduction in b. cereus spore numbers (5.6 log units) was achieved after a ho ...	2014	25393824
evaluation of antibacterial and anthelmintic activities with total phenolic contents of piper betel leaves.	the study was conducted to investigate the antibacterial and anthelmintic activities and to determine total phenolic contents of methanolic extract of piper betel leaves.	2014	25386394
antimicrobial activity of ammodaucus leucotrichus fruit oil from algerian sahara.	three fruit oil samples of ammodaucus leucotrichus cosson & durieu from algerian sahara were obtained by hydrodistillation and analyzed by gc(ri), gc-ms and 13c nmr spectroscopy. the main compounds were perillaldehyde (87.0-87.9%) and limonene (7.4-8.2%). the antimicrobial effect of the essential oil was evaluated against bacteria, yeasts and filamentous fungi. high antibacterial activity was observed against escherichia coli, staphylococcus aureus. enterobacter cloaceae, bacillus cereus and sal ...	2014	25026729
a new chemically defined medium for the growth and sporulation of bacillus cereus strains in anaerobiosis.	a new chemically defined liquid medium, mods, was developed for the aerobic growth and anaerobic growth and sporulation of bacillus cereus strains. the comparison of sporulation capacity of 18 strains of b. cereus has shown effective growth and spore production in anaerobiosis..	2014	25019521
bacillus cereus panophthalmitis associated with injection drug use.	we report a case of rapidly progressive vision loss in a young woman with a history of injection drug use. subsequent enucleation of the affected eye was done and bacillus cereus grew on tissue culture. b. cereus is a rare cause of endogenous endophthalmitis due to hematogenous seeding of the vitreous in the setting of injection drug use.	2014	25016038
direct evidence of iron uptake by the gram-positive siderophore-shuttle mechanism without iron reduction.	iron is an essential element for all organisms, and microorganisms produce small molecule iron-chelators, siderophores, to efficiently acquire fe(iii). gram-positive bacteria possess lipoprotein siderophore-binding proteins (sbps) on the membrane. some of the sbps bind both apo-siderophores (iron-free) and fe-siderophore (iron-chelated) and only import fe-siderophores. when the sbp initially binds an apo-siderophore, the sbp uses the gram-positive siderophore-shuttle mechanism (the sbps exchange ...	2014	25007174
statistical optimization of fibrinolytic enzyme production using agroresidues by bacillus cereus ind1 and its thrombolytic activity in vitro.	a potent fibrinolytic enzyme-producing bacillus cereus ind1 was isolated from the indian food, rice. solid-state fermentation was carried out using agroresidues for the production of fibrinolytic enzyme. among the substrates, wheat bran supported more enzyme production and has been used for the optimized enzyme production by statistical approach. two-level full-factorial design demonstrated that moisture, supplementation of beef extract, and sodium dihydrogen phosphate have significantly influen ...	2014	25003130