Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| simultaneous detection of entamoeba histolytica/dispar, giardia duodenalis and cryptosporidia by immunochromatographic assay in stool samples from patients living in the greater cairo region, egypt. | gastrointestinal infection due to intestinal parasites is an enormous health problem in developing countries and its reliable diagnosis is demanding. therefore, this study aimed at evaluating a commercially available immunochromatographic assay (ica) for the detection of cryptosporidia, giardia duodenalis, and entamoeba histolytica/dispar for its usefulness in the greater cairo region, egypt. stool samples of 104 patients who presented between october 2012 and march 2013 with gastrointestinal sy ... | 2015 | 26018117 |
| intestinal parasitic infections among mentally handicapped individuals in alexandria, egypt. | this cross-sectional study was carried to determine the prevalence of intestinal parasitic infections among mentally handicapped individuals in alexandria, egypt, in the period from december 2012 till november 2013. the study was conducted on 200 institutionalized and non-institutionalized mentally handicapped individuals. fresh stool samples were subjected to different stains including; trichrome for detecting intestinal protozoa, modified acid fast stain for intestinal coccidia and quick hot g ... | 2015 | 26878626 |
| intestinal parasites detected during pre-employment stool examination at tertiary health care center in makkah, kingdom of saudi arabia. | this retrospective study determined the intestinal parasites discovered in 2490 new employees in a tertiary health care center in makah as regards nationalities, age groups and sex over the period from january 2010 to january 2014 to decrease introduction of communicable diseases. all the data were provided through the electronic hospital information system. the overall positive cases were 16%, significantly higher in females 57.4% than males 42.6%. differences in distribution of positive record ... | 2015 | 26485855 |
| predicting frequency distribution and influence of sociodemographic and behavioral risk factors of schistosoma mansoni infection and analysis of co-infection with intestinal parasites. | geospatial analysis was used to study the epidemiology of schistosoma mansoni, intestinal parasites and co-infections in an area (ilha das flores) in sergipe, brazil. we collected individually georeferenced sociodemographic, behavioral and parasitological data from 500 subjects, analyzed them by conventional statistics, and produced risk maps by kernel estimation. the prevalence rates found were: s. mansoni (24.0%), trichuris trichiura (54.8%), ascaris lumbricoides (49.2%), hookworm (17.6%) and ... | 2015 | 26054512 |
| structure of uridine diphosphate n-acetylglucosamine pyrophosphorylase from entamoeba histolytica. | uridine diphosphate n-acetylglucosamine pyrophosphorylase (uap) catalyzes the final step in the synthesis of udp-glcnac, which is involved in cell-wall biogenesis in plants and fungi and in protein glycosylation. small-molecule inhibitors have been developed against uap from trypanosoma brucei that target an allosteric pocket to provide selectivity over the human enzyme. a 1.8 å resolution crystal structure was determined of uap from entamoeba histolytica, an anaerobic parasitic protozoan that c ... | 2015 | 25945709 |
| molecular and biochemical characterization of entamoeba histolytica fructokinase. | entamoeba histolytica is the causative agent of amoebic dysentery and liver abscess. the medium for its axenic culture contains glucose as energy source, and we addressed the question whether e. histolytica can also use fructose instead. as the amoebic hexokinases do not phosphorylate fructose, a separate fructokinase is essential. the genome project revealed a single candidate gene encoding an e. histolytica homolog of bacterial fructokinases. this gene was cloned, and the recombinant enzyme ha ... | 2015 | 25700717 |
| serological investigation to identify risk factors for post-flood infectious diseases: a longitudinal survey among people displaced by typhoon morakot in taiwan. | after typhoon morakot struck taiwan in 2009, thousands of taiwanese citizens were displaced to shelters for several weeks. others were placed in urban communities where they had family members. this study aimed to investigate serological status in both groups and identify risk factors associated with seroconversion of infectious diseases. | 2015 | 25976763 |
| discordances between serology and culture for strongyloides in an ethiopian adopted child with multiple parasitic infections: a case report. | infectious diseases screening of international adoptees is complex because of the concurrence of different pathogens in a child at same time. we describe an international adopted child born at ethiopia infected by 5 different pathogens (hymenolepis nana, giardia intestinalis, entamoeba histolytica, strongyloides stercoralis, and trichuris trichiura), 2 of them s. stercoralis and e. histolytica with a capacity to develop severe clinical complications if not detected promptly with appropriate diag ... | 2016 | 26962825 |
| the 12 gastrointestinal pathogens spectrum of acute infectious diarrhea in a sentinel hospital, shenzhen, china. | acute infectious gastroenteritis is one of the most common diseases among all ages, particularly in developing countries. the pathogen spectrum may differ among different regions and seasons. to investigate the etiology of acute diarrhea in shenzhen, a prospective study was conducted from august 2014 to september 2015. stools from 412 patients with diarrhea (286 of whom were adults) including the general epidemiological information of the patients were collected. the 19 pathogens were detected b ... | 2016 | 27965649 |
| a study of parasitic and bacterial pathogens associated with diarrhea in hiv-positive patients. | diarrhea is a common complication of acquired immune deficiency syndrome (aids), occurring in almost 90% of aids patients in developing countries like india. the present study was aimed to determine the prevalence and microbiological profile of pathogens associated with diarrhea in human immunodeficiency virus (hiv) positive patients and their relation to cd4 counts. | 2016 | 27795876 |
| a decade of intestinal protozoan epidemiology among settled immigrants in qatar. | the world health organization estimates that about 3.5 billion people worldwide are affected by intestinal parasitic infections. reports have already emphasized the role of immigrants in outbreaks of parasitic diseases in industrialized countries. with the mass influx of immigrants to qatar, patent intestinal parasitic infections have been observed. herein, the prevalence of intestinal protozoan infections was analysed in 29,286 records of subjects referred for stool examination at the hamad med ... | 2016 | 27496143 |
| irritable bowel syndrome and intestinal parasites: a view from south america. | irritable bowel syndrome (ibs) is a functional gastrointestinal disorder of uncertain etiology. several studies have proposed the possible role of intestinal parasites in the pathogenesis of ibs. we aimed to summarize the epidemiological studies that describe a possible link between intestinal parasites and ibs, with special interest in endemic areas for intestinal parasitism such as south america. a comprehensive review of the literature was conducted by using the keywords: irritable bowel synd ... | 2016 | 27409092 |
| effect of sanitation and water treatment on intestinal protozoa infection: a systematic review and meta-analysis. | pathogenic intestinal protozoa infections are responsible for substantial mortality and morbidity, particularly in settings where people lack improved sanitation and safe drinking water. we assessed the relation between access to, and use of, sanitation facilities and water treatment and infection with intestinal protozoa. | 2016 | 26404667 |
| children with moderate-high infection with entamoeba coli have higher percentage of body and abdominal fat than non-infected children. | intestinal parasites, virus and bacterial infections are positively associated with obesity and adiposity in vitro and in animal models, but conclusive evidence of this relationship in humans is lacking. the aim of this cross-sectional study was to determine differences in adiposity between infected and non-infected children, with a high prevalence of intestinal parasitic infection and obesity. | 2016 | 26663860 |
| prevalence and factors associated with intestinal parasitic infections among food handlers of southern ethiopia: cross sectional study. | globally about one third of the total population is estimated to be infected with intestinal parasites, of which, the majority are people living in tropical and sub-tropical parts of the world. cases of intestinal parasitosis are also highly abundant in ethiopia and hence the aim of present study was to assess prevalence and predictors of intestinal parasitic infections among food handlers working in arba minch university students' cafeteria, south ethiopia. | 2016 | 26831331 |
| household and personal factors are sources of heterogenity in intestinal parasite clearance among mexican children 6-15 months of age supplemented with vitamin a and zinc. | a randomised, double-blind, placebo-controlled trial was carried out among mexico children aged 6-15 months to determine how household characteristics modify vitamin a and zinc supplementation efficacy on ascaris lumbricoides, giardia intestinalis and entamoeba histolytica/e. dispar infection durations. children assigned to receive vitamin a every 2 months, a daily zinc supplement, a combined vitamin a-zinc supplement or a placebo were followed for 1 year. parametric hazard models were fit to in ... | 2016 | 26772449 |
| infectious diseases in immigrant population related to the time of residence in spain. | the aim of this study was to evaluate the data on the main imported infectious diseases and public health issues arising from the risk of transmission of tropical and common diseases in the immigrant population. during the period of study, 2,426 immigrants were attended in the tropical medicine unit of the hospital of poniente. for each patient, a complete screening for common and tropical diseases was performed. the prevalence and main features of intestinal and urinary parasites, microfilarias ... | 2016 | 25466580 |
| etiology of diarrhea in children younger than 5 years attending the bengo general hospital in angola. | diarrheal disease is among the leading causes of death in children younger than 5 years, especially in developing countries. the aim of this study was to investigate the most frequent etiological agents of diarrhea and its associated factors in children younger than 5 years attending the bengo general hospital in angola. | 2016 | 26761347 |
| marked seasonality of cyclospora cayetanensis infections: ten-year observation of hospital cases, honduras. | document seasonality occurrence and epidemiologic characteristics of cyclospora cayetanensis infections during a 10-year period from patients consulting at the university hospital, honduras. | 2016 | 26847438 |
| study of the gastrointestinal parasitic fauna of captive non-human primates (macaca fascicularis). | the aim of this study was to examine helminths and protozoans in cynomolgus macaques (macaca fascicularis) imported from registered breeding facilities in china and their relation to health risks for non-human primate handlers in biomedical research centers and in breeding facilities. fresh fecal samples were collected from a total of 443 m. fascicularis and analyzed by copromicroscopical analysis, immunoenzymatic, or molecular assays. as to helminths, whose eggs were shed in 2.03% of the sample ... | 2016 | 26374536 |
| structure of methionine γ-lyase from clostridium sporogenes. | methionine γ-lyase (mgl) is a pyridoxal 5'-phosphate-dependent enzyme that catalyzes the γ-elimination reaction of l-methionine. the enzyme is a promising target for therapeutic intervention in some anaerobic pathogens and has attracted interest as a potential cancer treatment. the crystal structure of mgl from clostridium sporogenes has been determined at 2.37 å resolution. the fold of the protein is similar to those of homologous enzymes from citrobacter freundii, entamoeba histolytica, pseudo ... | 2016 | 26750487 |
| risk of intestinal parasitic infections in people with different exposures to wastewater and fecal sludge in kampala, uganda: a cross-sectional study. | there are health risks associated with wastewater and fecal sludge management and use, but little is known about the magnitude, particularly in rapidly growing urban settings of low- and middle-income countries. we assessed the point-prevalence and risk factors of intestinal parasite infections in people with different exposures to wastewater and fecal sludge in kampala, uganda. | 2016 | 26938060 |
| evaluation of the nanochip® gastrointestinal panel (gip) test for simultaneous detection of parasitic and bacterial enteric pathogens in fecal specimens. | infectious gastroenteritis is a global health problem associated with high morbidity and mortality rates. rapid and accurate diagnosis is crucial to allow appropriate and timely treatment. current laboratory stool testing has a long turnaround time (tat) and demands highly qualified personnel and multiple techniques. the need for high throughput and the number of possible enteric pathogens compels the implementation of a molecular approach which uses multiplex technology, without compromising pe ... | 2016 | 27447173 |
| parasitic contamination of fresh vegetables sold at central markets in khartoum state, sudan. | fresh vegetables are considered as vital nutrients of a healthy diet as they supply the body with essential supplements. the consumption of raw vegetables is the main way for transmission of intestinal parasitic organisms. this study was aimed at detecting the parasitic contamination in fresh vegetables sold in two central open-aired markets in khartoum state, sudan. | 2016 | 26968696 |
| prevalence of intestinal parasite infections among patients in local public hospitals of hail, northwestern saudi arabia. | to evaluate the prevalence of intestinal parasites among patients in hail, northwestern saudi arabia. | 2016 | 26851785 |
| multi-parallel qpcr provides increased sensitivity and diagnostic breadth for gastrointestinal parasites of humans: field-based inferences on the impact of mass deworming. | although chronic morbidity in humans from soil transmitted helminth (sth) infections can be reduced by anthelmintic treatment, inconsistent diagnostic tools make it difficult to reliably measure the impact of deworming programs and often miss light helminth infections. | 2016 | 26813411 |
| identification of repressive and active epigenetic marks and nuclear bodies in entamoeba histolytica. | in human hosts, entamoeba histolytica cysts can develop into trophozoites, suggesting that the life cycle of this parasite are regulated by changes in gene expression. to date, some evidence has suggested that epigenetic mechanisms such as dna methylation and histone modification are involved in the regulation of gene expression in entamoeba. some post-translational modifications (ptms) at the n-terminus of e. histolytica's histones have been reported experimentally, including tri-methylation in ... | 2016 | 26767976 |
| prevalence of intestinal parasitic infections and associated risk factors among pregnant women attending antenatal care center at felege hiwot referral hospital, northwest ethiopia. | parasitic infections affect tens of millions of pregnant women worldwide, and directly or indirectly lead to a spectrum of adverse maternal and fetal/placental effects. the objective of this study was to assess the prevalence of intestinal parasite infections and associated risk factors among pregnant women attending antenatal care center in felege hiwot referral hospital, bahir dar city, northwest ethiopia. | 2016 | 27716099 |
| entamoeba histolytica trophozoites and lipopeptidophosphoglycan trigger human neutrophil extracellular traps. | neutrophil defense mechanisms include phagocytosis, degranulation and the formation of extracellular traps (net). these networks of dna are triggered by several immune and microbial factors, representing a defense strategy to prevent microbial spread by trapping/killing pathogens. this may be important against entamoeba histolytica, since its large size hinders its phagocytosis. the aim of this study was to determine whether e. histolytica and their lipopeptidophosphoglycan (ehlppg) induce the f ... | 2016 | 27415627 |
| comparison of nested-multiplex, taqman & sybr green real-time pcr in diagnosis of amoebic liver abscess in a tertiary health care institute in india. | amoebiasis is a common parasitic infection caused by entamoeba histolytica and amoebic liver abscess (ala) is the most common extraintestinal manifestation of amoebiasis. the aim of this study was to standardise real-time pcr assays (taqman and sybr green) to detect e. histolytica from liver abscess pus and stool samples and compare its results with nested-multiplex pcr. | 2016 | 26997014 |
| surveillance for parasites in unaccompanied minor refugees migrating to germany in 2015. | in 2015, most of the refugees arriving in germany originated from countries with poor hygienic and sanitary conditions. stool samples of 1,230 minor refugees unaccompanied by adults were investigated for possible parasites. giardia lamblia was by far the most frequently detected parasite (n=165); all other parasites were considerably less frequent and encountered in the following order: hymenolepis nana (n=23), entamoeba histolytica (n=17), trichuris trichiura (n=8), and blastocystis hominis (n= ... | 2016 | 26958459 |
| risk factors associated with parasitic infection among municipality solid-waste workers in an egyptian community. | solid-waste management is associated with several health hazards, particularly parasitic infection. the objective of the study was to determine the association between risk factors and the occurrence of intestinal parasitic infections (potentially pathogenic) among municipal waste collectors in alexandria, egypt. a cross-sectional survey was conducted in the main municipality company in alexandria. a total of 346 municipality solid-waste workers (mswws) was interviewed using an in-depth question ... | 2016 | 26606452 |
| ehnpc1 and ehnpc2 proteins participate in trafficking of exogenous cholesterol in entamoeba histolytica trophozoites: relevance for phagocytosis. | entamoeba histolytica, the highly phagocytic protozoan causative of human amoebiasis lacks the machinery to synthesize cholesterol. here, we investigated the presence of npc1 and npc2 proteins in this parasite, which are involved in cholesterol trafficking in mammals. bioinformatics analysis revealed one ehnpc1 and two ehnpc2 genes. ehnpc1 appeared as a transmembrane protein and both ehnpc2 as peripheral membrane proteins. molecular docking predicted that ehnpc1 and ehnpc2 bind cholesterol and i ... | 2016 | 28002502 |
| migrant workers in malaysia: current implications of sociodemographic and environmental characteristics in the transmission of intestinal parasitic infections. | a cross-sectional study of intestinal parasitic infections amongst migrant workers in malaysia was conducted. a total of 388 workers were recruited from five sectors including manufacturing, construction, plantation, domestic and food services. the majority were recruited from indonesia (n = 167, 43.3%), followed by nepal (n = 81, 20.9%), bangladesh (n = 70, 18%), india (n = 47, 12.1%) and myanmar (n = 23, 5.9.2%). a total of four nematode species (ascaris lumbricoides, trichuris trichiura, ente ... | 2016 | 27806046 |
| mouse models of amoebiasis and culture methods of amoeba. | entamoeba histolytica is the third leading parasitic cause of man mortality in the world. infection occurs via ingestion of food or water contaminated with cysts of e. histolytica. amoebae primarily colonize the intestine. the majority of amoebic infections are asymptomatic, but under some conditions, approximately 4-10% of infections progress to the invasive form of the disease. to better understand the pathogenesis of amoebiasis and the interaction between amoebae and their hosts, the developm ... | 2016 | 27080249 |
| entamoeba histolytica cysteine proteinase 5 evokes mucin exocytosis from colonic goblet cells via αvβ3 integrin. | critical to the pathogenesis of intestinal amebiasis, entamoeba histolytica (eh) induces mucus hypersecretion and degrades the colonic mucus layer at the site of invasion. the parasite component(s) responsible for hypersecretion are poorly defined, as are regulators of mucin secretion within the host. in this study, we have identified the key virulence factor in live eh that elicits the fast release of mucin by goblets cells as cysteine protease 5 (ehcp5) whereas, modest mucus secretion occurred ... | 2016 | 27073869 |
| balantidium coli liver abscess: first case report from india. | protozoal infections are common in the tropics. amoebic colitis is the commonest of these infections and can lead to liver abscess as a complication. balantidium coli is a rare free moving protozoal parasite which is known to infest human large intestine causing a type of colitis very similar to that caused by entamoeba histolytica. however this pathogen is not known to cause liver invasion in humans. we report here a case of liver abscess caused by b. coli, which is probably the first such case ... | 2016 | 27065613 |
| cellular gene expression induced by parasite antigens and allergens in neonates from parasite-infected mothers. | prenatal exposure to parasite antigens or allergens will influence the profile and strength of postnatal immune responses, such contact may tolerize and increase susceptibility to future infections or sensitize to environmental allergens. exposure in utero to parasite antigens will distinctly alter cellular gene expression in newborns. gene microarrays were applied to study gene expression in umbilical cord blood cell (ucbc) from parasite-exposed (para-pos) and non-exposed (para-neg) neonates. u ... | 2016 | 27062712 |
| identification and functional characterization of lysine methyltransferases of entamoeba histolytica. | lysine methylation of histones, a posttranslational modification catalyzed by lysine methyltransferases (hkmts), plays an important role in the epigenetic regulation of transcription. lysine methylation of non-histone proteins also impacts the biological function of proteins. previously it has been shown that lysine methylation of histones of entamoeba histolytica, the protozoan parasite that infects 50 million people worldwide each year and causing up to 100,000 deaths annually, is implicated i ... | 2016 | 27062489 |
| [a case of peristomal cutaneous ulcer following amebic colitis caused by entamoeba histolytica]. | a 66-year-old japanese male with a history of a rectal ulcer and rectovesical fistula following brachytherapy and radiotherapy for prostate cancer, who had undergone colostomy and vesicotomy presented with a painful peristomal ulcer of approximately 5 x 2.5cm adjacent to the direction of 6 o'clock of the stoma in his left lower abdomen. although he was admitted to be treated with intravenous antibiotics and topical debridement, the ulcer was rapidly increasing. in the laboratory findings, wbc wa ... | 2016 | 27032177 |
| data on docking and dynamics simulation of entamoeba histolytica ehadh (an alix protein) and lysobisphosphatidic acid. | entamoeba histolytica is the protozoan agent responsible for human amoebiasis. trophozoites are highly phagocytic cells and the lysobisphosphatidic acid (lbpa) is involved in endocytosis. lbpa interacts with ehadh protein (an alix family member) also participating in phagocytosis, as it is referred in the research article identification of the phospholipid lysobisphosphatidic acid in the protozoan entamoeba histolytica: an active molecule in endocytosis (castellanos-castro et al., 2016) [1]. to ... | 2016 | 27014730 |
| biophysical studies on calcium and carbohydrate binding to carbohydrate recognition domain of gal/galnac lectin from entamoeba histolytica: insights into host cell adhesion. | entamoeba histolytica, an enteric parasite expresses a gal/galnac-specific lectin that contributes to its virulence by establishing adhesion to host cell. in this study, carbohydrate recognition domain of hgl (ehcrd) was purified and biophysical studies were conducted to understand the thermodynamic basis of its binding to carbohydrate and ca(++) here, we show that carbohydrate recognition domain (crd) of the lectin binds to calcium through dpn motif. to decipher the role of calcium in carbohydr ... | 2016 | 27008865 |
| gut microbiome diversity in acute infective and chronic inflammatory gastrointestinal diseases in north india. | the disease profile in the indian population provides a unique opportunity for studying the host microbiome interaction in both infectious (amebiasis) and autoimmune diseases like inflammatory bowel disease (ibd) from a similar environment and genetic background. analysis of fecal samples from untreated amebic liver abscess (ala) patients, entamoeba histolytica (eh)-negative and -positive asymptomatic individuals, and pus samples from naive ala patients revealed a significant reduction in lactob ... | 2016 | 26994772 |
| epidemiology of domestically acquired amebiasis in japan, 2000-2013. | notifications of amebiasis have been increasing in japan. using national surveillance data during 2000-2013, reported cases of amebiasis were analyzed. a case of amebiasis was defined as laboratory-confirmed entamoeba histolytica infection, regardless of presence of symptoms. we described temporal trends and analyzed correlates of asymptomatic versus symptomatic cases based on odds ratios (ors) and 95% confidence intervals (cis) using logistic regression. of 9,946 cases reported during 2000-2013 ... | 2016 | 26976888 |
| infection strategies of intestinal parasite pathogens and host cell responses. | giardia lamblia, cryptosporidium sp., and entamoeba histolytica are important pathogenic intestinal parasites and are amongst the leading causes worldwide of diarrheal illness in humans. diseases caused by these organisms, giardiasis, cryptosporidiosis, and amoebiasis, respectively, are characterized by self-limited diarrhea but can evolve to long-term complications. the cellular and molecular mechanisms underlying the pathogenesis of diarrhea associated with these three pathogens are being unra ... | 2016 | 26973630 |
| identification of ehtif-ia: the putative e. histolytica orthologue of the human ribosomal rna transcription initiation factor-ia. | initiation of rdna transcription requires the assembly of a specific multi-protein complex at the rdna promoter containing the rna pol i with auxiliary factors. one of these factors is known as rrn3p in yeast and transcription initiation factor ia (tif-ia) in mammals. rrn3p/tif-ia serves as a bridge between rna pol i and the pre-initiation complex at the promoter. it is phosphorylated at multiple sites and is involved in regulation of rdna transcription in a growth-dependent manner. in the early ... | 2016 | 26949087 |
| roles of acetyl-coa synthetase (adp-forming) and acetate kinase (ppi-forming) in atp and ppi supply in entamoeba histolytica. | acetate is an end-product of the ppi-dependent fermentative glycolysis in entamoeba histolytica; it is synthesized from acetyl-coa by adp-forming acetyl-coa synthetase (acs) with net atp synthesis or from acetyl-phosphate by a unique ppi-forming acetate kinase (ack). the relevance of these enzymes to the parasite atp and ppi supply, respectively, are analyzed here. | 2016 | 26922831 |
| the cytokine profile of human nkt cells and pbmcs is dependent on donor sex and stimulus. | sex-related variations in natural killer t (nkt) cells may influence immunoregulation and outcome of infectious and autoimmune diseases. we analyzed sex-specific differences in peripheral blood nkts and peripheral blood mononuclear cells (pbmcs) from men and women and determined the frequencies of nkt cells and their subpopulations [cd4(+); cd8(+); double negative (dn)] and the levels of cytokine production following stimulation with the nkt cell ligands α-galactosylceramide (αgalcer) and entamo ... | 2016 | 26895635 |
| a review of the proposed role of neutrophils in rodent amebic liver abscess models. | host invasion by entamoeba histolytica, the pathogenic agent of amebiasis, can lead to the development of amebic liver abscess (ala). due to the difficulty of exploring host and amebic factors involved in the pathogenesis of ala in humans, most studies have been conducted with animal models (e.g., mice, gerbils, and hamsters). histopathological findings reveal that the chronic phase of ala in humans corresponds to lytic or liquefactive necrosis, whereas in rodent models there is granulomatous in ... | 2016 | 26880421 |
| x-ray structures of thioredoxin and thioredoxin reductase from entamoeba histolytica and prevailing hypothesis of the mechanism of auranofin action. | the anti-arthritic gold-containing drug auranofin is lethal to the protozoan intestinal parasite entamoeba histolytica, the causative agent of human amebiasis, in both culture and animal models of the disease. a putative mechanism of auranofin action proposes that monovalent gold, au(i), released from the drug, can bind to the redox-active dithiol group of thioredoxin reductase (trxr). au(i) binding in the active site is expected to prevent electron transfer to the downstream substrate thioredox ... | 2016 | 26876147 |
| human liver infection in a dish: easy-to-build 3d liver models for studying microbial infection. | human liver infection is a major cause of death worldwide, but fundamental studies on infectious diseases affecting humans have been hampered by the lack of robust experimental models that accurately reproduce pathogen-host interactions in an environment relevant for the human disease. in the case of liver infection, one consequence of this absence of relevant models is a lack of understanding of how pathogens cross the sinusoidal endothelial barrier and parenchyma. to fill that gap we elaborate ... | 2016 | 26863526 |
| first report of entamoeba histolytica infection from timor-leste--acute amoebic colitis and concurrent late development of amoebic liver abscess in returned travellers to australia. | this communication reports invasive amoebic colitis and late onset amoebic liver abscess in three members of a group of 12 australian travellers to timor-leste (tl). this is the first report of entamoeba histolytica infection from tl. clinicians in australia need to consider amoebiasis in the differential diagnosis in travellers returning with colitis, abdominal pain and fever. presentation with amoebic liver abscess months after exposure is rare but should be suspected in symptomatic individual ... | 2016 | 26858275 |
| intracellular traffic of the lysine and glutamic acid rich protein kerp1 reveals features of endomembrane organization in entamoeba histolytica. | the development of amoebiasis is influenced by the expression of the lysine and glutamic acid rich protein 1 (kerp1), a virulence factor involved in entamoeba histolytica adherence to human cells. up to date, it is unknown how the protein transits the parasite cytoplasm towards the plasma membrane, specially because this organism lacks a well-defined endoplasmic reticulum (er) and golgi apparatus. in this work we demonstrate that kerp1 is present at the cell surface and in intracellular vesicles ... | 2016 | 26857352 |
| protozoan parasites. | • stool antigen detection for cryptosporidium sp, giardia lamblia and entamoeba histolytica are now commercially available, have better sensitivity and specificity than the traditional stool microscopy, and are less dependent on personnel skill. tests employing newer techniques with faster turnaround time are also available for diagnosing trichomoniasis.• nitazoxanide, the only u.s. food and drug administration-approved medication for therapy of cryptosporidiosis, is effective among immunocompet ... | 2016 | 26834225 |
| evaluation of the c-terminal fragment of entamoeba histolytica gal/galnac lectin intermediate subunit as a vaccine candidate against amebic liver abscess. | entamoeba histolytica is an intestinal protozoan parasite that causes amoebiasis, including amebic dysentery and liver abscesses. e. histolytica invades host tissues by adhering onto cells and phagocytosing them depending on the adaptation and expression of pathogenic factors, including gal/galnac lectin. we have previously reported that e. histolytica possesses multiple cxxc sequence motifs, with the intermediate subunit of gal/galnac lectin (i.e., igl) as a key factor affecting the amoeba's pa ... | 2016 | 26824828 |
| application of a multiplex quantitative pcr to assess prevalence and intensity of intestinal parasite infections in a controlled clinical trial. | accurate quantitative assessment of infection with soil transmitted helminths and protozoa is key to the interpretation of epidemiologic studies of these parasites, as well as for monitoring large scale treatment efficacy and effectiveness studies. as morbidity and transmission of helminth infections are directly related to both the prevalence and intensity of infection, there is particular need for improved techniques for assessment of infection intensity for both purposes. the current study ai ... | 2016 | 26820626 |
| knockdown of five genes encoding uncharacterized proteins inhibits entamoeba histolytica phagocytosis of dead host cells. | entamoeba histolytica is the protozoan parasite that causes invasive amebiasis, which is endemic to many developing countries and characterized by dysentery and liver abscesses. the virulence of e. histolytica correlates with the degree of host cell engulfment, or phagocytosis, and e. histolytica phagocytosis alters amebic gene expression in a feed-forward manner that results in an increased phagocytic ability. here, we used a streamlined rna interference screen to silence the expression of 15 g ... | 2016 | 26810036 |
| il-23 prevents il-13-dependent tissue repair associated with ly6c(lo) monocytes in entamoeba histolytica-induced liver damage. | the il-23/il-17 axis plays an important role in the pathogenesis of autoimmune diseases and the pathological consequences of infection. we previously showed that immunopathologic mechanisms mediated by inflammatory monocytes underlie the severe focal liver damage induced by the protozoan parasite, entamoeba histolytica. here, we analyze the contribution of the il-23/il-17 axis to the induction and subsequent recovery from parasite-induced liver damage. | 2016 | 26809113 |
| endoplasmic reticulum-resident rab8a gtpase is involved in phagocytosis in the protozoan parasite entamoeba histolytica. | phagocytosis is indispensable for the pathogenesis of the intestinal protozoan parasite entamoeba histolytica. here, we showed that in e. histolytica rab8a, which is generally involved in trafficking from the trans-golgi network to the plasma membrane in other organisms but was previously identified in phagosomes of the amoeba in the proteomic analysis, primarily resides in the endoplasmic reticulum (er) and participates in phagocytosis. we demonstrated that down-regulation of ehrab8a by small a ... | 2016 | 26807810 |
| solution structure of the calmodulin-like c-terminal domain of entamoeba α-actinin2. | cell motility is dependent on a dynamic meshwork of actin filaments that is remodelled continuously. a large number of associated proteins that are severs, cross-links, or caps the filament ends have been identified and the actin cross-linker α-actinin has been implied in several important cellular processes. in entamoeba histolytica, the etiological agent of human amoebiasis, α-actinin is believed to be required for infection. to better understand the role of α-actinin in the infectious process ... | 2016 | 26800385 |
| multinucleation and polykaryon formation is promoted by the ehpc4 transcription factor in entamoeba histolytica. | entamoeba histolytica is the intestinal parasite responsible for human amoebiasis that is a leading cause of death in developing countries. in this protozoan, heterogeneity in dna content, polyploidy and genome plasticity have been associated to alterations in mechanisms controlling dna replication and cell division. studying the function of the transcription factor ehpc4, we unexpectedly found that it is functionally related to dna replication, and multinucleation. site-directed mutagenesis on ... | 2016 | 26792358 |
| screening and discovery of lineage-specific mitosomal membrane proteins in entamoeba histolytica. | entamoeba histolytica, an anaerobic intestinal parasite causing dysentery and extra-intestinal abscesses in humans, possesses highly reduced and divergent mitochondrion-related organelles (mros) called mitosomes. this organelle lacks many features associated with canonical aerobic mitochondria and even other mros such as hydrogenosomes. the entamoeba mitosome has been found to have a compartmentalized sulfate activation pathway, which was recently implicated to have a role in amebic stage conver ... | 2016 | 26792249 |
| development of rna interference trigger-mediated gene silencing in entamoeba invadens. | entamoeba histolytica, a protozoan parasite, is an important human pathogen and a leading parasitic cause of death. the organism has two life cycle stages, trophozoites, which are responsible for tissue invasion, and cysts, which are involved in pathogen transmission. entamoeba invadens is the model system to study entamoeba developmental biology, as high-grade regulated encystation and excystation are readily achievable. however, the lack of gene-silencing tools in e. invadens has limited the m ... | 2016 | 26787723 |
| entamoeba thiol-based redox metabolism: a potential target for drug development. | amebiasis is an intestinal infection widespread throughout the world caused by the human pathogen entamoeba histolytica. metronidazole has been a drug of choice against amebiasis for decades despite its low efficacy against asymptomatic cyst carriers and emergence of resistance in other protozoa with similar anaerobic metabolism. therefore, identification and characterization of specific targets is urgently needed to design new therapeutics for improved treatment against amebiasis. toward this g ... | 2016 | 26775086 |
| technical advances in trigger-induced rna interference gene silencing in the parasite entamoeba histolytica. | entamoeba histolytica has a robust endogenous rna interference (rnai) pathway. there are abundant 27 nucleotide (nt) anti-sense small rnas (as srnas) that target genes for silencing and the genome encodes many genes involved in the rnai pathway such as argonaute proteins. importantly, an e. histolytica gene with numerous as srnas can function as a "trigger" to induce silencing of a gene that is fused to the trigger. thus, the amebic rnai pathway regulates gene expression relevant to amebic biolo ... | 2016 | 26747561 |
| autophosphorylation at thr279 of entamoeba histolytica atypical kinase ehak1 is required for activity and regulation of erythrophagocytosis. | phagocytosis plays a key role in survival and pathogenicity of entamoeba histolytica. we have recently demonstrated that an atypical kinase ehak1 is involved in phagocytosis in this parasite. it is recruited to the phagocytic cups through interaction with ehcabp1. ehak1 manipulates actin dynamics by multiple mechanisms including phosphorylation of g-actin. biochemical analysis showed that ehak1 is a serine/threonine kinase with broad ion specificity and undergoes multiple trans-autophosphorylati ... | 2016 | 26739245 |
| proteomic identification of oxidized proteins in entamoeba histolytica by resin-assisted capture: insights into the role of arginase in resistance to oxidative stress. | entamoeba histolytica is an obligate protozoan parasite of humans, and amebiasis, an infectious disease which targets the intestine and/or liver, is the second most common cause of human death due to a protozoan after malaria. although amebiasis is usually asymptomatic, e. histolytica has potent pathogenic potential. during host infection, the parasite is exposed to reactive oxygen species that are produced and released by cells of the innate immune system at the site of infection. the ability o ... | 2016 | 26735309 |
| resveratrol induces apoptosis-like death and prevents in vitro and in vivo virulence of entamoeba histolytica. | entamoeba histolytica causes amoebiasis, an infection that kills 100,000 individuals each year. metronidazole and its derivatives are currently used against this protozoan, but these drugs present adverse effects on human health. here, we investigated the effect of resveratrol (a natural compound) on e. histolytica trophozoites viability, as well as its influence on the parasite virulence. trophozoites growth was arrested by 72 μm resveratrol and the ic50 was determined as 220 μm at 48 h. cells ... | 2016 | 26731663 |
| prevalence of entamoeba histolytica, giardia lamblia, and cryptosporidium spp. in libya: 2000-2015. | introduction the intestinal protozoa entamoeba histolytica, giardia lamblia, and cryptosporidium spp. are the causative agents of giardiasis, amebiasis, and cryptosporidiosis, respectively. adequate knowledge of the geographical distribution of parasites and the demographic variables that influence their prevalence is important for effective control of infection in at-risk populations. methods the data were obtained by an english language literature search of medline and pubmed for papers using ... | 2016 | 28349839 |
| role of the gut microbiota of children in diarrhea due to the protozoan parasite entamoeba histolytica. | an estimated 1 million children die each year before their fifth birthday from diarrhea. previous population-based surveys of pediatric diarrheal diseases have identified the protozoan parasite entamoeba histolytica, the etiological agent of amebiasis, as one of the causes of moderate-to-severe diarrhea in sub-saharan africa and south asia. | 2016 | 26712950 |
| the knockdown of each component of the cysteine proteinase-adhesin complex of entamoeba histolytica (ehcpadh) affects the expression of the other complex element as well as the in vitro and in vivo virulence. | entamoeba histolytica is the protozoan parasite causative of human amoebiasis, disease responsible for 40 000-100 000 deaths annually. the cysteine proteinase-adhesin complex of this parasite (ehcpadh) is a heterodimeric protein formed by a cysteine protease (ehcp112) and an adhesin (ehadh) that plays an important role in the cytopathic mechanism of this parasite. the coding genes for ehcp112 and ehadh are adjacent in the e. histolytica genome, suggesting that their expression may be co-regulate ... | 2016 | 26521708 |
| crystal structure analysis of wild type and fast hydrolyzing mutant of ehrabx3, a tandem ras superfamily gtpase from entamoeba histolytica. | the enteric protozoan parasite, entamoeba histolytica, is the causative agent of amoebic dysentery, liver abscess and colitis in human. vesicular trafficking plays a key role in the survival and virulence of the protozoan and is regulated by various rab gtpases. ehrabx3 is a catalytically inefficient amoebic rab protein, which is unique among the eukaryotic ras superfamily by virtue of its tandem domain organization. here, we report the crystal structures of gdp-bound fast hydrolyzing mutant (v7 ... | 2016 | 26555751 |
| activity, stability and folding analysis of the chitinase from entamoeba histolytica. | human amebiasis, caused by the parasitic protozoan entamoeba histolytica, remains as a significant public health issue in developing countries. the life cycle of the parasite compromises two main stages, trophozoite and cyst, linked by two major events: encystation and excystation. interestingly, the cyst stage has a chitin wall that helps the parasite to withstand harsh environmental conditions. since the amebic chitinase, ehcht1, has been recognized as a key player in both encystation and excy ... | 2016 | 26526675 |
| characterization of a human isolate of tritrichomonas foetus (cattle/swine genotype) infected by a zoonotic opportunistic infection. | tritrichomonas species flagellates (imc strain) were isolated from the biliary tract of an individual who had developed cholecystitis as a complication of acquired agammaglobulinemia. sequence analysis of tritrichomonas sp. (imc clone 2 (cl2)) was performed for several genetic regions including the its1-5.8s rdna-its2 region, the cysteine protease (cp)-1, cp-2 and cp-4 to cp-9 genes, and the cytosolic malate dehydrogenase 1 gene. in addition to comparison of the variable-length dna repeats in th ... | 2016 | 26685985 |
| diagnostic parameters of serological elisa for invasive amoebiasis, using antigens preserved without enzymatic inhibitors. | amoebiasis is the third cause of death due to parasites in the world. although, numerous serodiagnostic and salivary tests have been developed, the majority of these assays lack sensitivity in endemic zones to detect acute amoebic liver abscess. the two main limiting factors to develop reliable assays are the high levels of anti-amoeba antibodies in populations living in endemic zones, and the proteolysis of amoebic extracts even treated with inhibitors. our group reported a method to preserve a ... | 2016 | 26684288 |
| extensive transcriptome analysis correlates the plasticity of entamoeba histolytica pathogenesis to rapid phenotype changes depending on the environment. | amoebiasis is a human infectious disease due to the amoeba parasite entamoeba histolytica. the disease appears in only 20% of the infections. diversity in phenotypes may occur within the same infectious strain in the gut; for instance, parasites can be commensal (in the intestinal lumen) or pathogenic (inside the tissue). the degree of pathogenesis of clinical isolates varies greatly. these findings raise the hypothesis that genetic derivation may account for amoebic diverse phenotypes. the main ... | 2016 | 27767091 |
| role of ehrab7a in phagocytosis of type 1 fimbriated e. coli by entamoeba histolytica. | entamoeba histolytica, the causative agent of amoebic colitis and liver abscess in human, ingests the intestinal bacteria and variety of host cells. phagocytosis of bacteria by the amebic trophozoite has been reported to be important for the virulence of the parasite. here, we set out to characterize different stages of phagocytosis of type 1 e. coli and investigated the role of a set of amoebic rab gtpases in the process. the localizations of the rab gtpases during different stages of the phago ... | 2016 | 27663892 |
| entamoeba encystation: new targets to prevent the transmission of amebiasis. | amebiasis is caused by entamoeba histolytica infection and can produce a broad range of clinical signs, from asymptomatic cases to patients with obvious symptoms. the current epidemiological and clinical statuses of amebiasis make it a serious public health problem worldwide. the entamoeba life cycle consists of the trophozoite, the causative agent for amebiasis, and the cyst, the form responsible for transmission. these two stages are connected by "encystation" and "excystation." hence, develop ... | 2016 | 27764256 |
| dispensability of the [4fe-4s] cluster in novel homologues of adenine glycosylase muty. | 7,8-dihydro-8-deoxyguanine (8og) is one of the most common oxidative lesions in dna. dna polymerases misincorporate an adenine across from this lesion. thus, 8og is a highly mutagenic lesion responsible for g:c→t:a transversions. muty is an adenine glycosylase, part of the base excision repair pathway that removes adenines, when mispaired with 8og or guanine. its catalytic domain includes a [4fe-4s] cluster motif coordinated by cysteinyl ligands. when this cluster is absent, muty activity is dep ... | 2016 | 26613369 |
| novel aryl carbamate derivatives of metronidazole as potential antiamoebic agents. | a series of novel aryl carbamate derivatives of metronidazole (mnz) were designed, synthesized, and screened for antiamoebic activity. as compared to mnz, most of the derivatives exhibited moderate to excellent activity against the hm1:imss strain of entamoeba histolytica. compounds 7, 14, 16, 19, and 21 exhibited the most promising antiamoebic activity with ic50 values of 0.24, 0.08, 0.26, 0.26, and 0.15 μm, respectively, compared to that of mnz (1.78 μm). moreover, from the toxicological studi ... | 2016 | 26597858 |
| the oxygen reduction pathway and heat shock stress response are both required for entamoeba histolytica pathogenicity. | several species belonging to the genus entamoeba can colonize the mouth or the human gut; however, only entamoeba histolytica is pathogenic to the host, causing the disease amoebiasis. this illness is responsible for one hundred thousand human deaths per year worldwide, affecting mainly underdeveloped countries. throughout its entire life cycle and invasion of human tissues, the parasite is constantly subjected to stress conditions. under in vitro culture, this microaerophilic parasite can toler ... | 2016 | 26589893 |
| evaluation of the bd max enteric parasite panel for clinical diagnostics. | we compared the performance of the bd max enteric parasite panel to routine microscopy and an in-house pcr for the detection of giardia intestinalis, entamoeba histolytica, and cryptosporidium spp. the enteric parasite panel showed good specificity for all targets and good sensitivity for e. histolytica and cryptosporidium spp. sensitivity for g. intestinalis with the bd max enteric parasite panel was equivalent to that with microscopy. | 2016 | 26582832 |
| human parasitic protozoan infection to infertility: a systematic review. | protozoan parasitic diseases are endemic in many countries worldwide, especially in developing countries, where infertility is a major burden. it has been reported that such infections may cause infertility through impairment in male and female reproductive systems. we searched medline, pubmed, and scopus databases and google scholar to identify the potentially relevant studies on protozoan parasitic infections and their implications in human and animal model infertility. literature described th ... | 2016 | 26573517 |
| the motility of entamoeba histolytica: finding ways to understand intestinal amoebiasis. | the pathogenic amoeba entamoeba histolytica is able to migrate within various compartments of the human body. the present article reviews progress in understanding the mechanisms of cell motility in e. histolytica during human intestinal invasion and, in particular, how the three-dimensional characteristics of the environment regulate the parasite's behaviour. the amoeboid mode of migration that applies to e. histolytica's displacements on two-dimensional surfaces is also expected to apply to th ... | 2016 | 27497052 |
| n-acetyl ornithine deacetylase is a moonlighting protein and is involved in the adaptation of entamoeba histolytica to nitrosative stress. | adaptation of the entamoeba histolytica parasite to toxic levels of nitric oxide (no) that are produced by phagocytes may be essential for the establishment of chronic amebiasis and the parasite's survival in its host. in order to obtain insight into the mechanism of e. histolytica's adaptation to no, e. histolytica trophozoites were progressively adapted to increasing concentrations of the no donor drug, s-nitrosoglutathione (gsno) up to a concentration of 110 μm. the transcriptome of no adapte ... | 2016 | 27808157 |
| identification of entamoeba histolytica and e. dispar infection in maceió, alagoas state, northeast brazil. | a cross-sectional study was carried out to assess the prevalence of e. histolytica and e. dispar by examining stool samples obtained from 1,003 students of public schools in maceió, alagoas, brazil. | 2016 | 27801380 |
| recent progress on the discovery of antiamoebic agents. | a large number of protozoans infect humans but entamoeba histolytica is the only organism responsible for causing amoebiasis, a deadly disease after malaria. numerous heterocycle-based antiamoebic agents have been previously synthesized as e. histolytica inhibitors and while some of these agents have shown moderate activity, the search for a novel and ideal antiamoebic compound is still ongoing. in this digest letter, we present the latest data on antiamoebic agents from 2011 to 2016 based on th ... | 2016 | 27707603 |
| a new nucleocytoplasmic rhogap protein contributes to control the pathogenicity of entamoeba histolytica by regulating ehracc and ehracd activity. | small gtpases are signalling molecules that regulate important cellular processes. gtpases are deactivated by gtpase-activating proteins (gaps). while human gaps have been intensively studied, no gap has yet been characterized in entamoeba histolytica. in this study, we identified and characterized a novel nucleocytoplasmic rhogap in e. histolytica termed ehrhogapnc. in silico analyses of the domain structure revealed a previously undescribed peptide region within the carboxy-terminal region of ... | 2016 | 27107405 |
| cardioprotective effects of monocyte locomotion inhibitory factor on myocardial ischemic injury by targeting vimentin. | monocyte locomotion inhibitory factor (mlif), a heat-stable pentapeptide produced by entamoeba histolytica, has anti-inflammatory function and protective effect on ischemic stroke. in this study, we evaluated the effect of mlif on myocardial ischemia. mice were subjected to ischemia/reperfusion by occlusion of the left anterior descending artery (lad). after sacrifice, the serum concentrations of cardiac troponin i (ctni), creatine kinase (ck), lactate dehydrogenase (ldh) as well as the heart in ... | 2016 | 27773717 |
| recent advances in entamoeba biology: rna interference, drug discovery, and gut microbiome. | in recent years, substantial progress has been made in understanding the molecular and cell biology of the human parasite entamoeba histolytica, an important pathogen with significant global impact. this review outlines some recent advances in the entamoeba field in the last five years, focusing on areas that have not recently been discussed in detail: (i) molecular mechanisms regulating parasite gene expression, (ii) new efforts at drug discovery using high-throughput drug screens, and (iii) th ... | 2016 | 27853522 |
| efficiency of direct microscopy of stool samples using an antigen-specific adhesin test for entamoeba histolytica. | e. histolytica is among the common causes of acute gastroenteritis. the pathogenic species e. histolytica and the nonpathogenic species e. dispar cannot be morphologically differentiated, although correct identification of these protozoans is important for treatment and public health. in many laboratories, the screening of leukocytes, erythrocytes, amoebic cysts, trophozoites and parasite eggs is performed using native-lugol's iodine for pre-diagnosis. | 2016 | 27761283 |
| recurrent amebic liver abscesses over a 16-year period: a case report. | amebic liver abscess is a rare disease in high-income countries. recurrence of amebic liver abscess is even rarer with only a few previous reports. here we present a patient who developed three subsequent amebic liver abscesses over a sixteen-year period. | 2016 | 27756400 |
| prevalence of intestinal parasitic infections and associated risk factors among schoolchildren in the plateau central and centre-ouest regions of burkina faso. | unsafe drinking water, unimproved sanitation and lack of hygiene pose health risks, particularly to children in low- and middle-income countries. this study aimed to assess the prevalence and risk factors of intestinal parasitic infections in school-aged children in two regions of burkina faso. | 2016 | 27756339 |
| the potential of lactobacillus casei and entercoccus faecium combination as a preventive probiotic against entamoeba. | travellers' diarrhoea caused by enteric protozoa like entamoeba histolytica is among the most common protozoan diseases in developing countries. in developing countries, amoebiasis is the second most prevalent protozoan disease. this protozoan parasite is often known to coexist as a part of the normal gut microbiota. it is estimated that around 50-60 % of population in developing countries might be harbouring entamoeba in an asymptomatic manner. due to physiological perturbation or upon immuno-c ... | 2016 | 27709564 |
| intestinal parasitic infections and associated epidemiological drivers in two rural communities of the bolivian chaco. | in 2013 a coproparasitological survey was carried out in two rural communities of the bolivian chaco to determine the prevalence of intestinal parasitic infections (ipis) and to investigate on possible infection drivers through a questionnaire interview. | 2016 | 27694735 |
| role of cysteine residues in the redox-regulated oligomerization and nucleotide binding to ehrabx3. | the enteric protozoan parasite, entamoeba histolytica, an etiological agent of amebiasis, is involved in the adhesion and destruction of human tissues. worldwide, the parasite causes about 50 million cases of amebiasis and 100,000 deaths annually. ehrabx3, a unique amoebic rab gtpase with tandem g-domains, possesses an unusually large number of cysteine residues in its n-terminal domain. crystal structure of ehrabx3 revealed an intra-molecular disulfide bond between c39 and c163 which is critica ... | 2016 | 27485554 |
| primary pulmonary amebiasis complicated with multicystic empyema. | amebiasis is a parasitic infection caused by the protozoan entamoeba histolytica. while most infections are asymptomatic, the disease could manifest clinically as amebic dysentery and/or extraintestinal invasion in the form of amebic liver abscess or other more rare manifestations such as pulmonary, cardiac, or brain involvement. herein we are reporting a case of a 24-year-old male with history of down syndrome who presented with severe right side pneumonia complicated with multicystic empyema r ... | 2016 | 27478673 |
| fulminant amebic colitis after corticosteroid therapy: a systematic review. | amebic colitis, caused by intestinal infection with the parasite, entamoeba histolytica, is a common cause of diarrhea worldwide. fulminant amebic colitis is the most devastating complication of this infection, associated with both high mortality and morbidity. we conducted a review of the english literature to describe cases of fulminant amebic colitis associated with exposure to corticosteroid medications in order to identify the risk factors for poor outcome and determine difficulties in diag ... | 2016 | 27467600 |
| modulation of innate immune response by the vagus nerve in experimental hepatic amebiasis in rats. | the parasympathetic nervous system has a crucial role in immunomodulation of the vagus nerve, its structure provides a pathogen detection system, and a negative feedback to the immune system after the pathogenic agent has been eliminated. amebiasis is a disease caused by the protozoan parasite entamoeba histolytica, considered the third leading cause of death in the world. the rats are used as a natural resistance model to amoebic liver infection. the aim of this study is to analyze the interact ... | 2016 | 27466057 |
| role of serum amyloid a, granulocyte-macrophage colony-stimulating factor, and bone marrow granulocyte-monocyte precursor expansion in segmented filamentous bacterium-mediated protection from entamoeba histolytica. | intestinal segmented filamentous bacteria (sfb) protect from ameba infection, and protection is transferable with bone marrow dendritic cells (bmdcs). sfb cause an increase in serum amyloid a (saa), suggesting that saa might mediate sfb's effects on bmdcs. here we further explored the role of bone marrow in sfb-mediated protection. transient gut colonization with sfb or saa administration alone transiently increased the h3k27 histone demethylase jmjd3, persistently increased bone marrow csf2ra e ... | 2016 | 27456830 |