Publications

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stable isotope labeling assisted liquid chromatography-electrospray tandem mass spectrometry for quantitative analysis of endogenous gibberellins.in the current study, we developed a stable isotope labeling strategy for the absolute quantification of gibberellins (gas) by high performance liquid chromatography-electrospray tandem mass spectrometry (hplc-esi-ms/ms). n,n-dimethyl ethylenediamine (dmed) and its deuterated counterpart d(4)-dmed were used to derivatize gas extracted from plant tissue samples and ga standards respectively. the both derivatives of gas were mixed and then subjected to hplc-esi-ms/ms analysis. the absolute quantif ...201526452831
2d-dige-based proteome expression changes in leaves of rice seedlings exposed to low-level gamma radiation at iitate village, fukushima.the present study continues our previous research on investigating the biological effects of low-level gamma radiation in rice at the heavily contaminated iitate village in fukushima, by extending the experiments to unraveling the leaf proteome. 14-days-old plants of japonica rice (oryza sativa l. cv. nipponbare) were subjected to gamma radiation level of upto 4 µsv/h, for 72 h. following exposure, leaf samples were taken from the around 190 µsv/3 d exposed seedling and total proteins were extra ...201526451896
divergent dna methylation patterns associated with gene expression in rice cultivars with contrasting drought and salinity stress response.dna methylation is an epigenetic mechanism that play an important role in gene regulation in response to environmental conditions. the understanding of dna methylation at the whole genome level can provide insights into the regulatory mechanisms underlying abiotic stress response/adaptation. we report dna methylation patterns and their influence on transcription in three rice (oryza sativa) cultivars (ir64, stress-sensitive; nagina 22, drought-tolerant; pokkali, salinity-tolerant) via an integra ...201526449881
alanine aminotransferase 1 (osalaat1) plays an essential role in the regulation of starch storage in rice endosperm.alteration of storage substances, in particular the major storage form starch, leads to floury endosperm. because floury mutants have physical attributes for milling processes, identification and characterization of those mutants are valuable. in this study we identified a floury endosperm mutant caused by a t-dna insertion in oryza sativa alanine-aminotransferase1 (osalaat1). osalaat1 is localized in the cytosol and has aminotransferase enzyme activity. the osalaat1 mutant has less amylose and ...201526475189
genome-wide cataloging and analysis of alternatively spliced genes in cereal crops.protein functional diversity at the post-transcriptional level is regulated through spliceosome mediated pre-mrna alternative splicing (as) events and that has been widely demonstrated to be a key player in regulating the functional diversity in plants. identification and analysis of as genes in cereal crop plants are critical for crop improvement and understanding regulatory mechanisms.201526391769
map-based cloning of seed dormancy1-2 identified a gibberellin synthesis gene regulating the development of endosperm-imposed dormancy in rice.natural variation in seed dormancy is controlled by multiple genes mapped as quantitative trait loci in major crop or model plants. this research aimed to clone and characterize the seed dormancy1-2 (qsd1-2) locus associated with endosperm-imposed dormancy and plant height in rice (oryza sativa). qsd1-2 was delimited to a 20-kb region, which contains osga20ox2 and had an additive effect on germination. naturally occurring or induced loss-of-function mutations of the gibberellin (ga) synthesis ge ...201526373662
identification of four functionally important microrna families with contrasting differential expression profiles between drought-tolerant and susceptible rice leaf at vegetative stage.developing drought-tolerant rice varieties with higher yield under water stressed conditions provides a viable solution to serious yield-reduction impact of drought. understanding the molecular regulation of this polygenic trait is crucial for the eventual success of rice molecular breeding programmes. micrornas have received tremendous attention recently due to its importance in negative regulation. in plants, apart from regulating developmental and physiological processes, micrornas have also ...201526369665
osmapk6, a mitogen-activated protein kinase, influences rice grain size and biomass production.grain size is an important agronomic trait in determining grain yield. however, the molecular mechanisms that determine the final grain size are not well understood. here, we report the functional analysis of a rice (oryza sativa l.) mutant, dwarf and small grain1 (dsg1), which displays pleiotropic phenotypes, including small grains, dwarfism and erect leaves. cytological observations revealed that the small grain and dwarfism of dsg1 were mainly caused by the inhibition of cell proliferation. m ...201526366992
advanced backcross qtl analysis reveals complicated genetic control of rice grain shape in a japonica × indica cross.grain shape is an important trait for improving rice yield. a number of quantitative trait loci (qtls) for this trait have been identified by using primary f2 mapping populations and recombinant inbred lines, in which qtls with a small effect are harder to detect than they would be in advanced generations. in this study, we developed two advanced mapping populations (chromosome segment substitution lines [cssls] and bc4f2 lines consisting of more than 2000 individuals) in the genetic backgrounds ...201526366113
characterization of rice (oryza sativa l.) genotypes on the basis of morpho-physiological and biochemical traits grown under aerobic situation in rainfed ecosystem .the objective of the present study was to examine the effect of aerobic situation on yield, physiological and biochemical traits of advanced breeding lines of rice. experiment was conducted with two set of rice genotypes under two water regimes (aerobic and irrigated), during three consecutive wet seasons 2010-2012. significant decrease in yield was observed in rice genotypes grown under aerobic situation as compared to the irrigated ones. promising rice genotypes having the ability to maintain ...201526364481
real time pcr expression analysis of gene encoding p5cs enzyme and proline metabolism under naci salinity in rice.regulation of proline accumulation in seedlings of rice (oryza sativa l. cv. lunishree) was investigated. the increasing concentration of nacl from 85 mm to 425 mm nacl progressively increased proline content in rice. the maximum increase in proline content was recorded at 425 mm nacl concentration as compared to control and other concentrations of nacl. the highest significant activity of proline synthesizing enzymes, delta1-pyrrolline-5-carboxylate synthetase, delta1-pyrrolline-5-carboxylate r ...201526364475
diterpenes from the roots of oryza sativa l. and their inhibition activity on no production in lps-stimulated raw264.7 macrophages.two new pimarane diterpenoids, momilactone d (3) and momilactone e (5), along with three known diterpenoids, momilactone a (1), sandaracopimaradien-3-one (2), and oryzalexin a (4) were isolated from oryza sativa roots. the chemical structures of the compounds were determined by spectroscopic data analysis. the isolated diterpenoids were evaluated for their ability to inhibit no production and inos mrna and protein expression in lps-stimulated raw264.7 macrophages. compound 4 showed strong inhibi ...201526363880
effects of cadmium on bioaccumulation and biochemical stress response in rice (oryza sativa l.).this study investigated the effects of various cd concentrations on the bioaccumulation, antioxidative defense, and stress responses of rice (oryza sativa l.). the distribution characteristics of cd in rice were in the following order: roots>stems>grains. the bioconcentration factor values of cd increased at concentrations lower than 3.00 mg cd/kg and approximately decreased to a constant value at concentrations higher than 3.00 mg cd/kg. rice showed a higher cd accumulation potential at low cd ...201526363148
proteomic and phosphoproteomic analyses reveal extensive phosphorylation of regulatory proteins in developing rice anthers.anther development, particularly around the time of meiosis, is extremely crucial for plant sexual reproduction. meanwhile, cell-to-cell communication between somatic (especial tapetum) cells and meiocytes are important for both somatic anther development and meiosis. to investigate possible molecular mechanisms modulating protein activities during anther development, we applied high-resolution mass spectrometry-based proteomic and phosphoproteomic analyses for developing rice (oryza sativa) ant ...201526360816
molecular dissection of a rice microtubule-associated ring finger protein and its potential role in salt tolerance in arabidopsis.although a number of ring e3 ligases in plants have been demonstrated to play key roles in a wide range of abiotic stresses, relatively few studies have detailed how ring e3 ligases exert their cellular actions. we describe oryza sativa ring finger protein with microtubule-targeting domain 1 (osrmt1), a functional ring e3 ligase that is likely involved in a salt tolerance mechanism. functional characterization revealed that osrmt1 undergoes homodimer formation and subsequently autoubiquitination ...201526358044
biallelic and genome wide association mapping of germanium tolerant loci in rice (oryza sativa l.).rice plants accumulate high concentrations of silicon. silicon has been shown to be involved in plant growth, high yield, and mitigating biotic and abiotic stresses. however, it has been demonstrated that inorganic arsenic is taken up by rice through silicon transporters under anaerobic conditions, thus the ability to efficiently take up silicon may be considered either a positive or a negative trait in rice. germanium is an analogue of silicon that produces brown lesions in shoots and leaves, a ...201526356220
small-scale gene duplications played a major role in the recent evolution of wheat chromosome 3b.bread wheat is not only an important crop, but its large (17 gb), highly repetitive, and hexaploid genome makes it a good model to study the organization and evolution of complex genomes. recently, we produced a high quality reference sequence of wheat chromosome 3b (774 mb), which provides an excellent opportunity to study the evolutionary dynamics of a large and polyploid genome, specifically the impact of single gene duplications.201526353816
distinct regulatory changes underlying differential expression of teosinte branched1-cycloidea-proliferating cell factor genes associated with petal variations in zygomorphic flowers of petrocosmea spp. of the family gesneriaceae.cycloidea (cyc)-like genes, belonging to the plant-specific tcp transcription factor family that is named after teosinte branched1 (tb1) from maize (zea mays), cyc from antirrhinum majus, and the proliferating cell factors (pcf) from rice (oryza sativa), have conserved dorsal identity function in patterning floral zygomorphy mainly through specific expression in dorsal petals of a flower. their expression changes are usually related to morphological diversity of zygomorphic flowers. however, it ...201526351309
epigenetic mutation of rav6 affects leaf angle and seed size in rice.heritable epigenetic variants of genes, termed epialleles, can broaden genetic and phenotypic diversity in eukaryotes. epialleles may also provide a new source of beneficial traits for crop breeding, but very few epialleles related to agricultural traits have been identified in crops. here, we identified epi-rav6, a gain-of-function epiallele of rice (oryza sativa) related to abscisic acid insensitive3 (abi3)/viviparous1 (vp1) 6 (rav6), which encodes a b3 dna-binding domain-containing protein. t ...201526351308
sustained low abscisic acid levels increase seedling vigor under cold stress in rice (oryza sativa l.).stress-induced abscisic acid (aba) is mainly catabolized by aba 8'-hydroxylase (aba8ox), which also strictly regulates endogenous aba levels. although three members of the aba8ox gene family are conserved in rice, it is not clear which stressors induce expression of these genes. here, we found that osaba8ox1 was induced by cold stress within 24 h and that osaba8ox2 and osaba8ox3 were not. in contrast, osaba8ox2 and osaba8ox3 were aba-inducible, but osaba8ox1 was not. osaba8ox1, osaba8ox2, and os ...201526350634
divergent biochemical and enzymatic properties of oxalate oxidase isoforms encoded by four similar genes in rice.the biochemical and enzymatic properties of four highly similar rice oxalate oxidase proteins (osoxo1-4) were compared after their purification from the leaves of transgenic plants each overexpressing the respective osoxo1-4 genes. although alignment of their amino acid sequences has revealed divergence mainly in the signal peptides and they catalyze the same enzymic (oxalate oxidase) reaction, divergence in apparent molecular mass, km, optimum ph, stability and responses to inhibitors and activ ...201526347131
expression of drought tolerance genes in tropical upland rice cultivars (oryza sativa).gene expression related to drought response in the leaf tissues of two brazilian upland cultivars, the drought-tolerant douradão and the drought-sensitive primavera, was analyzed. rna-seq identified 27,618 transcripts in the douradão cultivar, with 24,090 (87.2%) homologous to the rice database, and 27,221 transcripts in the primavera cultivar, with 23,663 (86.9%) homologous to the rice database. gene-expression analysis between control and water-deficient treatments revealed 493 and 1154 differ ...201526345744
tiling assembly: a new tool for reference annotation-independent transcript assembly and novel gene identification by rna-sequencing.annotation of the rice (oryza sativa) genome has evolved significantly since release of its draft sequence, but it is far from complete. several published transcript assembly programmes were tested on rna-sequencing (rna-seq) data to determine their effectiveness in identifying novel genes to improve the rice genome annotation. cufflinks, a popular assembly software, did not identify all transcripts suggested by the rna-seq data. other assembly software was cpu intensive, lacked documentation, o ...201526341416
differential activation of genes related to aluminium tolerance in two contrasting rice cultivars.rice (oryza sativa) is a highly al-tolerant crop. among other mechanisms, a higher expression of star1/star2 (sensitive to al rhizotoxicity1/2) genes and of nrat1 (nramp aluminium transporter 1), and als1 (aluminium sensitive 1) can at least in part be responsible for the inducible al tolerance in this species. here we analysed the responses to al in two contrasting rice varieties. all analysed toxicity/tolerance markers (root elongation, evans blue, morin and haematoxylin staining) indicated hi ...201526337117
enlightenment on the aequorin-based platform for screening arabidopsis stress sensory channels related to calcium signaling.free calcium ions (ca(2+)) are an important signal molecule in response to a large array of external stimuli encountered by plants. using the aequorin-based ca(2+) recording system, tremendous progress has been made in understanding the ca(2+) responses to biotic or abiotic stresses in dicotyledonous arabidopsis. however, due to the lack of a similar detection system, little information has been obtained from the monocotyledonous rice (oryza sativa). recombinant aequorin has been introduced into ...201526336841
a node-localized transporter oszip3 is responsible for the preferential distribution of zn to developing tissues in rice.developing tissues such as meristem with low transpiration require high zn levels for their active growth, but the molecular mechanisms underlying the preferential distribution to these tissues are poorly understood. we found that a member of the zip (zrt, irt-like protein), oszip3, showed high expression in the nodes of rice (oryza sativa). immunostaining revealed that oszip3 was localized at the xylem intervening parenchyma cells and xylem transfer cells of the enlarged vascular bundle in both ...201526332571
strong shift in the diazotrophic endophytic bacterial community inhabiting rice (oryza sativa) plants after flooding.flooding impacts soil microbial communities, but its effect on endophytic communities has rarely been explored. this work addresses the effect of flooding on the abundance and diversity of endophytic diazotrophic communities on rice plants established in a greenhouse experiment. the nifh gene was significantly more abundant in roots after flooding, whereas the nifh gene copy numbers in leaves were unaffected and remained low. the pca (principal component analysis) of t-rflp (terminal restriction ...201526324852
in vitro assessment of allergenicity features and localization of probable ige binding regions.rice is cultivated as a staple grain crop in many countries, especially in asia. in the present study, recombinant rice chitinase was expressed, purified and characterized by in silico and immunobiochemical methods. rice chitinase was affinity purified and it resolved at 24 kda on sds-page. purified protein was analyzed for pepsin resistance, heat stability, and ige binding using atopic patients' sera. chitinase was resistant to pepsin digestion and heat treatment at 90 °c for 1 h. it showed sig ...201526321724
rice mel2, the rna recognition motif (rrm) protein, binds in vitro to meiosis-expressed genes containing u-rich rna consensus sequences in the 3'-utr.post-transcriptional gene regulation by rna recognition motif (rrm) proteins through binding to cis-elements in the 3'-untranslated region (3'-utr) is widely used in eukaryotes to complete various biological processes. rice meiosis arrested at leptotene2 (mel2) is the rrm protein that functions in the transition to meiosis in proper timing. the mel2 rrm preferentially associated with the u-rich rna consensus, uuaguu[u/a][u/g][a/u/g]u, dependently on sequences and proportionally to mel2 protein a ...201526319516
genome-wide association mapping of quantitative trait loci (qtls) for contents of eight elements in brown rice (oryza sativa l.).an association mapping of quantitative trait loci (qtls) regulating the concentrations of eight elements in brown rice (oryza sativa l.) was performed using usda mini-core subset cultivated in two different environments. in addition, correlation between the grain elemental concentrations was also studied. a total of 60 marker loci associated with 8 grain elemental concentrations were identified, and these loci were clustered into 37 genomic regions. twenty new qtls were found to be associated wi ...201526317332
[influence of sulfur on the formation of fe-mn plaque on root and uptake of cd by rice (oryza sativa l.)].a pool culture experiment using exogenous cd-polluted paddy soils was carried out to investigate the influence of different forms of sulfur fertilizers (sulfur and gypsum) on the formation of fe-mn plaque on rice root and the uptake of cd by rice. the results showed that the redox potential ( eh) was about--200-100 mv, the ph was 6.9-7.9 and the pe + ph was 4-10 in different growth periods of rice. the mass fractions of fe and mn plaque on rice root were 5000-13,000 mg · kg(-1) and 170-580 mg · ...201526314143
expansion of the mexican rice borer (lepidoptera: crambidae) into rice and sugarcane in louisiana.the mexican rice borer, eoreuma loftini (dyar) (lepidoptera: crambidae), is an invasive pest of sugarcane, saccharum spp., rice, oryza sativa l., and other graminaceous crops in the gulf coast region of the united states. traps baited with e. loftini female sex pheromones were used to document establishment and distribution of e. loftini near sugarcane, rice, and noncrop hosts in seven southwest louisiana parishes from 2009 to 2013. additional field surveys documented larval infestations in comm ...201526313982
aquaporin plays an important role in mediating chloroplastic co2 concentration under high-n supply in rice (oryza sativa) plants.our previous studies demonstrated that chloroplastic co2 concentration (cc) is not sufficient under high-nitrogen (n) supply in rice plants. in this research, we studied how aquaporins- (aqps) mediated cc under different n-supply levels. a hydroponic experiment was conducted in a greenhouse with three different n levels (low n, 0.71 mm; intermediate n, 2.86 mm; and high n, 7.14 mm) in a rice cultivar (oryza sativa cv. shanyou 63) and with an ospip1;1 mutant (oryza sativa cv. nipponbare). the pho ...201526382720
the interaction between rice erf3 and wox11 promotes crown root development by regulating gene expression involved in cytokinin signaling.crown roots are the main components of the fibrous root system in rice (oryza sativa). wox11, a wuschel-related homeobox gene specifically expressed in the emerging crown root meristem, is a key regulator in crown root development. however, the nature of wox11 function in crown root development has remained elusive. here, we identified a rice ap2/erf protein, erf3, which interacts with wox11 and was expressed in crown root initials and during crown root growth. functional analysis revealed that ...201526307379
arbuscular mycorrhizal symbiosis ameliorates the optimum quantum yield of photosystem ii and reduces non-photochemical quenching in rice plants subjected to salt stress.rice is the most important food crop in the world and is a primary source of food for more than half of the world population. however, salinity is considered the most common abiotic stress reducing its productivity. soil salinity inhibits photosynthetic processes, which can induce an over-reduction of the reaction centres in photosystem ii (psii), damaging the photosynthetic machinery. the arbuscular mycorrhizal (am) symbiosis may improve host plant tolerance to salinity, but it is not clear how ...201526291919
patatin-related phospholipase a, pplaiiiα, modulates the longitudinal growth of vegetative tissues and seeds in rice.patatin-related phospholipase a (ppla) hydrolyses glycerolipids to produce fatty acids and lysoglycerolipids. the oryza sativa genome has 21 putative pplas that are grouped into five subfamilies. overexpression of ospplaiiiα resulted in a dwarf phenotype with decreased length of rice stems, roots, leaves, seeds, panicles, and seeds, whereas ospplaiiiα-knockout plants had longer panicles and seeds. ospplaiiiα-overexpressing plants were less sensitive than wild-type and knockout plants to gibberel ...201526290597
assessment of issr based molecular genetic diversity of hassawi rice in saudi arabia.inter simple sequence repeat (issr) analysis, using 14 primers was performed to estimate genetic diversity among 27 landraces of hassawi rice growing in al-ahsa region of saudi arabia and deposited at king abdulaziz city for science and technology with kacst ids. the average polymorphism produced by 11 selected primers was more than 75%. the analysis of issr polymorphism divided the examined rice landraces into two groups; in one group (a), one accession (kacst 191) was clearly delimited as a di ...201526288564
route and regulation of zinc, cadmium, and iron transport in rice plants (oryza sativa l.) during vegetative growth and grain filling: metal transporters, metal speciation, grain cd reduction and zn and fe biofortification.zinc (zn) and iron (fe) are essential but are sometimes deficient in humans, while cadmium (cd) is toxic if it accumulates in the liver and kidneys at high levels. all three are contained in the grains of rice, a staple cereal. zn and fe concentrations in rice grains harvested under different levels of soil/hydroponic metals are known to change only within a small range, while cd concentrations show greater changes. to clarify the mechanisms underlying such different metal contents, we synthesiz ...201526287170
functional characterization of drought-responsive modules and genes in oryza sativa: a network-based approach.drought is one of the major environmental stress conditions affecting the yield of rice across the globe. unraveling the functional roles of the drought-responsive genes and their underlying molecular mechanisms will provide important leads to improve the yield of rice. co-expression relationships derived from condition-dependent gene expression data is an effective way to identify the functional associations between genes that are part of the same biological process and may be under similar tra ...201526284112
substrate specificity and possible heterologous targets of phytaspase, a plant cell death protease.plants lack aspartate-specific cell death proteases homologous to animal caspases. instead, a subtilisin-like serine-dependent plant protease named phytaspase shown to be involved in the accomplishment of programmed death of plant cells is able to hydrolyze a number of peptide-based caspase substrates. here, we determined the substrate specificity of rice (oryza sativa) phytaspase by using the positional scanning substrate combinatorial library approach. phytaspase was shown to display an absolu ...201526283788
overexpression of acetylcholinesterase gene in rice results in enhancement of shoot gravitropism.acetylcholine (ach), a known neurotransmitter in animals and acetylcholinesterase (ache) exists widely in plants, although its role in plant signal transduction is unclear. we previously reported ache in zea mays l. might be related to gravitropism based on pharmacological study using an ache inhibitor. here we clearly demonstrate plant ache play an important role as a positive regulator in the gravity response of plants based on a genetic study. first, the gene encoding a second component of th ...201526277389
natural variation in the flag leaf morphology of rice due to a mutation of the narrow leaf 1 gene in oryza sativa l.we investigated the natural variations in the flag leaf morphology of rice. we conducted a principal component analysis based on nine flag leaf morphology traits using 103 accessions from the national institute of agrobiological sciences core collection. the first component explained 39% of total variance, and the variable with highest loading was the width of the flag leaf (wfl). a genome-wide association analysis of 102 diverse japanese accessions revealed that marker rm6992 on chromosome 4 wa ...201526275424
phytochrome b mediates the regulation of chlorophyll biosynthesis through transcriptional regulation of chlh and gun4 in rice seedlings.accurate regulation of chlorophyll synthesis is crucial for chloroplast formation during the greening process in angiosperms. in this study, we examined the role of phytochrome b (phyb) in the regulation of chlorophyll synthesis in rice seedlings (oryza sativa l.) through the characterization of a pale-green phenotype observed in the phyb mutant grown under continuous red light (rc) irradiation. our results show that the rc-induced chlorophyll accumulation can be divided into two components--a p ...201526270815
x-ray computed tomography to study rice (oryza sativa l.) panicle development.computational tomography is an important technique for developing digital agricultural models that may help farmers and breeders for increasing crop quality and yield. in the present study an attempt has been made to understand rice seed development within the panicle at different developmental stages using this technique. during the first phase of cell division the hounsfield unit (hu) value remained low, increased in the dry matter accumulation phase, and finally reached a maximum at the matur ...201526265763
a stress-responsive nac transcription factor snac3 confers heat and drought tolerance through modulation of reactive oxygen species in rice.adverse environmental conditions such as high temperature and drought stress greatly limit the growth and production of crops worldwide. several nac (nam, ataf1/2, and cuc2) proteins have been documented as important regulators in stress responses, but the molecular mechanisms are largely unknown. here, a stress-responsive nac gene, snac3 (onac003, loc_os01g09550), conferring drought and heat tolerance in rice is reported. snac3 was ubiquitously expressed and its transcript level was induced by ...201526261267
loss of function of osmads3 via the insertion of a novel retrotransposon leads to recessive male sterility in rice (oryza sativa).natural mutation is the source of natural variation, which is the fundamental basis for the genetic improvement of crops. during the process of developing a recombinant inbred line (ri), a spontaneous mutagenesis in ri127 led to the production of the recessive male-sterile line ri127s. via a map-based cloning approach, the gene controlling the male sterility was identified as osmads3, which was previously reported to be associated with floral organ development and male sterility. thermal asymmet ...201526259187
a decrease in phytic acid content substantially affects the distribution of mineral elements within rice seeds.phytic acid (myo-inositol hexakisphosphate; insp6) is the storage compound of phosphorus and many mineral elements in seeds. to determine the role of insp6 in the accumulation and distribution of mineral elements in seeds, we performed fine mappings of mineral elements through synchrotron-based x-ray microfluorescence analysis using developing seeds from two independent low phytic acid (lpa) mutants of rice (oryza sativa l.). the reduced insp6 in lpa seeds did not affect the translocation of min ...201526259185
functional characterization of a transition metal ion transporter, oszip6 from rice (oryza sativa l.).micronutrients are important for the growth and development of plants, which deploy families of transporters for their uptake and distribution. we have functionally characterized a novel transition metal ion transporter from rice, oszip6 (oryza sativa zinc regulated transporter, iron regulated transporter-like protein 6). the transporter was found to be transcriptionally activated in shoot and root tissues in response to deficiency in fe(2+), zn(2+) and mn(2+). oszip6 was expressed in xenopus la ...201526476396
disruption of the rice nitrate transporter osnpf2.2 hinders root-to-shoot nitrate transport and vascular development.plants have evolved to express some members of the nitrate transporter 1/peptide transporter family (npf) to uptake and transport nitrate. however, little is known of the physiological and functional roles of this family in rice (oryza sativa l.). here, we characterized the vascular specific transporter osnpf2.2. functional analysis using cdna-injected xenopus laevis oocytes revealed that osnpf2.2 is a low-affinity, ph-dependent nitrate transporter. use of a green fluorescent protein tagged osnp ...201525923512
transport function of rice amino acid permeases (aaps).the transport function of four rice (oryza sativa) amino acid permeases (aaps), osaap1 (os07g04180), osaap3 (os06g36180), osaap7 (os05g34980) and osaap16 (os12g08090), was analyzed by expression in xenopus laevis oocytes and electrophysiology. osaap1, osaap7 and osaap16 functioned, similarly to arabidopsis aaps, as general amino acid permeases. osaap3 had a distinct substrate specificity compared with other rice or arabidopsis aaps. osaap3 transported the basic amino acids lysine and arginine we ...201525907566
confirming a major qtl and finding additional loci responsible for field resistance to brown spot (bipolaris oryzae) in rice.brown spot is a devastating rice disease. quantitative resistance has been observed in local varieties (e.g., 'tadukan'), but no economically useful resistant variety has been bred. using quantitative trait locus (qtl) analysis of recombinant inbred lines (rils) from 'tadukan' (resistant) × 'hinohikari' (susceptible), we previously found three qtls (qbs2, qbs9, and qbs11) that conferred resistance in seedlings in a greenhouse. to confirm their effect, the parents and later generations of rils we ...201526069447
silicon induces resistance to the brown spot fungus cochliobolus miyabeanus by preventing the pathogen from hijacking the rice ethylene pathway.although numerous studies have shown the ability of silicon (si) to mitigate a wide variety of abiotic and biotic stresses, relatively little is known about the underlying mechanism(s). here, we have investigated the role of hormone defense pathways in si-induced resistance to the rice brown spot fungus cochliobolus miyabeanus. to delineate the involvement of multiple hormone pathways, a multidisciplinary approach was pursued, combining exogenous hormone applications, pharmacological inhibitor e ...201525625327
over-expression of a glutamate dehydrogenase gene, mggdh, from magnaporthe grisea confers tolerance to dehydration stress in transgenic rice.heterologous expression of a fungal nadp(h)-gdh gene ( mggdh ) from magnaporthe grisea can improve dehydration stress tolerance in rice by preventing toxic accumulation of ammonium. glutamate dehydrogenase (gdh; ec 1.4.1.2 and ec 1.4.1.4) may act as a stress-responsive enzyme in detoxification of high intracellular ammonia and production of glutamate for proline synthesis under stress conditions. in present study, a fungal nadp(h)-gdh gene (mggdh) from magnaporthe grisea was over-expressed in ri ...201525486886
transcriptomic analysis and the expression of disease-resistant genes in oryza meyeriana under native condition.oryza meyeriana (o. meyeriana), with a gg genome type (2n = 24), accumulated plentiful excellent characteristics with respect to resistance to many diseases such as rice shade and blast, even immunity to bacterial blight. it is very important to know if the diseases-resistant genes exist and express in this wild rice under native conditions. however, limited genomic or transcriptomic data of o. meyeriana are currently available. in this study, we present the first comprehensive characterization ...201526640944
two distinct classes of protein related to gtb and rrm are critical in the sclerotial metamorphosis process of rhizoctonia solani ag-1 ia.sheath blight of rice, caused by rhizoctonia solani kühn ag-1 ia [teleomorph: thanatephorus cucumeris (frank) donk], is one of the major diseases of rice (oryza sativa l.) worldwide. sclerotia produced by r. solani ag-1 ia are crucial for their survival in adverse environments and further dissemination when environmental conditions become conducive. differentially expressed genes during three stages of sclerotial metamorphosis of r. solani ag-1 ia were investigated by utilizing complementary dna ...201525763752
draft genome sequence of the rice kernel smut tilletia horrida strain qb-1.tilletia horrida is the most destructive fungal pathogen of rice (oryza sativa l.). the 20,105,270-bp draft genome sequence of t. horrida strain qb-1 is reported here. genes encoding proteins associated with key virulence factors were predicted, and this can provide information for understanding the pathogenic mechanisms in t. horrida.201526112782
main ustilaginoidins and their distribution in rice false smut balls.rice false smut has become an increasingly serious fungal disease in rice (oryza sativa l.) production worldwide. ustilaginoidins are bis-naphtho-γ-pyrone mycotoxins previously isolated from the rice false smut balls (fsbs) infected by the pathogen villosiclava virens in rice spikelets on panicles. to investigate the main ustilaginoidins and their distribution in rice fsbs, five main bis-naphtho-γ-pyrones, namely ustilaginoidins a (1), g (2), b (3), i (4) and c (5), were isolated and identified ...201526473920
root-derived short-chain suberin diacids from rice and rape seed in a paddy soil under rice cultivar treatments.suberin-derived substituted fatty acids have been shown to be potential biomarkers for plant-derived carbon (c) in soils across ecosystems. analyzing root derived suberin compounds bound in soil could help to understand the root input into a soil organic carbon pool. in this study, bound lipids were extracted and identified in root and topsoil samples. short-chain suberin diacids were quantified under rice (oryza sativa l.) and rape (brassica campestris) rotations with different cultivar combina ...201525961557
genome-wide analysis of dof family transcription factors and their responses to abiotic stresses in chinese cabbage.chinese cabbage is an important leaf vegetable that experienced long-term cultivation and artificial selection. dof (dna-binding one zinc finger) transcription factors, with a highly conserved dof domain, are members of a major plant-specific transcription factor family that play important roles in many plant biological processes. the dof family transcription factors, one of the most important families of transcriptional regulators in higher plants, are involved in massive aspects of plant growt ...201525636232
combinations of the ghd7, ghd8 and hd1 genes largely define the ecogeographical adaptation and yield potential of cultivated rice.rice cultivars have been adapted to favorable ecological regions and cropping seasons. although several heading date genes have separately made contributions to this adaptation, the roles of gene combinations are still unclear. we employed a map-based cloning approach to isolate a heading date gene, which coordinated the interaction between ghd7 and ghd8 to greatly delay rice heading. we resequenced these three genes in a germplasm collection to analyze natural variation. map-based cloning demon ...201526147403
map-based cloning and characterization of bph29, a b3 domain-containing recessive gene conferring brown planthopper resistance in rice.rice (oryza sativa l.) production, essential for global food security, is threatened by the brown planthopper (bph). the breeding of host-resistant crops is an economical and environmentally friendly strategy for pest control, but few resistance gene resources have thus far been cloned. an indica rice introgression line rbph54, derived from wild rice oryza rufipogon, has been identified with sustainable resistance to bph, which is governed by recessive alleles at two loci. in this study, a map-b ...201526136269
laba1, a domestication gene associated with long, barbed awns in wild rice.common wild rice (oryza rufipogon), the wild relative of asian cultivated rice (oryza sativa), flaunts long, barbed awns, which are necessary for efficient propagation and dissemination of seeds. by contrast, o. sativa cultivars have been selected to be awnless or to harbor short, barbless awns, which facilitate seed processing and storage. the transition from long, barbed awns to short, barbless awns was a crucial event in rice domestication. here, we show that the presence of long, barbed awns ...201526082172
genetic analysis of seed-shattering genes in rice using an f₃:₄ population derived from an oryza sativa x oryza rufipogon cross.seed shattering of wild plant species is thought to be an adaptive trait to facilitate seed dispersal. for rice breeding, seed shatter-ing is an important trait for improving breeding strategies, particularly when developing lines use interspecific hybrids and introgression of genes from wild species. we developed f₃:₄ recombinant inbred lines from an interspecific cross between oryza sativa cv. ilpoombyeo and oryza rufipogon. in this study, we genetically analyzed known shat-tering-related loci ...201525730074
pcr-based indel markers co-dominant between oryza sativa, japonica cultivars and closely-related wild oryza species.wild relatives genetically close to cultivars are precious genetic resources for plant breeding. oryza rufipogon, o. barthii, o. glumaepatula, o. meridionalis and o. longistaminata are such wild species, and are also categorized as aa genome species based on their structural similarities. chromosome segment substitution lines (cssls) are a powerful resource in breeding and genetics, and numerous rice cssls have been produced. this study aimed to develop dna markers for evaluation of cssls direct ...201526366120
the birth of a black rice gene and its local spread by introgression.the origin and spread of novel agronomic traits during crop domestication are complex events in plant evolution. wild rice (oryza rufipogon) has red grains due to the accumulation of proanthocyanidins, whereas most cultivated rice (oryza sativa) varieties have white grains induced by a defective allele in the rc basic helix-loop-helix (bhlh) gene. although the events surrounding the origin and spread of black rice traits remain unknown, varieties with black grains due to anthocyanin accumulation ...201526362607
an-2 encodes a cytokinin synthesis enzyme that regulates awn length and grain production in rice.a wide range of morphological and physiological traits have changed between cultivated rice oryza sativa and wild rice oryza rufipogon under domestication. here, we report cloning of the an-2 gene, encoding the lonely guy like protein 6 (oslogl6), which catalyzes the final step of cytokinin synthesis in o. rufipogon. the near-isogenic line harboring a wild allele of an-2 in the genetic background of the awnless indica guangluai 4 shows that an-2 promotes awn elongation by enhancing cell division ...201526283047
mapping of seed shattering loci provides insights into origin of weedy rice and rice domestication.seed shattering is an important trait that distinguishes crop cultivars from the wild and weedy species. the genetics of seed shattering was investigated in this study to provide insights into rice domestication and the evolution of weedy rice. quantitative trait locus (qtl) analysis, conducted in 2 recombinant inbred populations involving 2 rice cultivars and a weedy rice accession of the southern united states, revealed 3-5 qtls that controlled seed shattering with 38-45% of the total phenotyp ...201524336929
the biosynthesis, structure and gelatinization properties of starches from wild and cultivated african rice species (oryza barthii and oryza glaberrima).the molecular structure and gelatinization properties of starches from domesticated african rice (oryza glaberrima) and its wild progenitor (oryza barthii) are determined and comparison made with asian domesticated rice (oryza sativa), the commonest commercial rice. this suggests possible enzymatic processes contributing to the unique traits of the african varieties. these have similar starch structures, including smaller amylose molecules, but larger amounts of amylose chains across the whole a ...201526050893
genome-wide survey and expression analysis of the osca gene family in rice.reception of and response to exogenous and endogenous osmotic changes is important to sustain plant growth and development, as well as reproductive formation. hyperosmolality-gated calcium-permeable channels (osca) were first characterised as an osmosensor in arabidopsis and are involved in the perception of extracellular changes to trigger hyperosmolality-induced [ca(2+)]i increases (oici). to explore the potential biological functions of oscas in rice, we performed a bioinformatics and express ...201526503287
convergent loss of awn in two cultivated rice species oryza sativa and oryza glaberrima is caused by mutations in different loci.a long awn is one of the distinct morphological features of wild rice species. this organ is thought to aid in seed dispersal and prevent predation by animals. most cultivated varieties of oryza sativa and oryza glaberrima, however, have lost the ability to form long awns. the causal genetic factors responsible for the loss of awn in these two rice species remain largely unknown. here, we evaluated three sets of chromosome segment substitution lines (cssls) in a common o. sativa genetic backgrou ...201526338659
fine mapping of s37, a locus responsible for pollen and embryo sac sterility in hybrids between oryza sativa l. and o. glaberrima steud.hybrid sterility locus s37 between oryza glaberrima and oryza sativa results in both pollen and embryo sac sterility. interspecific crossing between african cultivated rice oryza glaberrima and oryza sativa cultivars is hindered by hybrid sterility. to dissect the mechanism of interspecific hybrid sterility, we developed a near-isogenic line (nil)-s37 using dianjingyou1 (djy1) as the recipient parent and an african cultivated rice variety as the donor parent. empty pollen and embryo sac sterilit ...201526169392
do nerica rice cultivars express resistance to striga hermonthica (del.) benth. and striga asiatica (l.) kuntze under field conditions?the parasitic weeds striga asiatica and striga hermonthica cause high yield losses in rain-fed upland rice in africa. two resistance classes (pre- and post-attachment) and several resistant genotypes have been identified among nerica (new rice for africa) cultivars under laboratory conditions (in vitro) previously. however, little is known about expression of this resistance under field conditions. here we investigated (1) whether resistance exhibited under controlled conditions would express un ...201526089591
genome-wide comparison of asian and african rice reveals high recent activity of dna transposons.dna (class ii) transposons are ubiquitous in plant genomes. however, unlike for (class i) retrotransposons, only little is known about their proliferation mechanisms, activity, and impact on genomes. asian and african rice (oryza sativa and o. glaberrima) diverged approximately 600,000 years ago. their fully sequenced genomes therefore provide an excellent opportunity to study polymorphisms introduced from recent transposon activity.201525954322
rapid turnover of antimicrobial-type cysteine-rich protein genes in closely related oryza genomes.defensive and reproductive protein genes undergo rapid evolution. small, cysteine-rich secreted peptides (crps) act as antimicrobial agents and function in plant intercellular signaling and are over-represented among reproductively expressed proteins. because of their roles in defense, reproduction and development and their presence in multigene families, crp variation can have major consequences for plant phenotypic and functional diversification. we surveyed the crp genes of six closely relate ...201525842177
gc-ei-tof-ms analysis of in vivo carbon-partitioning into soluble metabolite pools of higher plants by monitoring isotope dilution after 13co2 labelling.the established gc-ei-tof-ms method for the profiling of soluble polar metabolites from plant tissue was employed for the kinetic metabolic phenotyping of higher plants. approximately 100 typical gc-ei-ms mass fragments of trimethylsilylated and methoxyaminated metabolite derivatives were structurally interpreted for mass isotopomer analysis, thus enabling the kinetic study of identified metabolites as well as the so-called functional group monitoring of yet non-identified metabolites. the monit ...201517475294
quantitative profiling of feruloylated arabinoxylan side-chains from graminaceous cell walls.graminaceous arabinoxylans are distinguished by decoration with feruloylated monosaccharidic and oligosaccharidic side-chains. although it is hypothesized that structural complexity and abundance of these feruloylated arabinoxylan side-chains may contribute, among other factors, to resistance of plant cell walls to enzymatic degradation, quantitative profiling approaches for these structural units in plant cell wall materials have not been described yet. here we report the development and applic ...201526834763
growth and cadmium phytoextraction by swiss chard, maize, rice, noccaea caerulescens, and alyssum murale in ph adjusted biosolids amended soils.past applications of biosolids to soils at some locations added higher cd levels than presently permitted. cadmium phytoextraction would alleviate current land use constraints. unamended farm soil, and biosolids amended farm and mine soils were obtained from a fulton co., il biosolids management facility. soils contained 0.16, 22.8, 45.3 mg cd kg(-1) and 43.1, 482, 812 mg zn kg(-1) respectively with initial ph 6.0, 6.1, 6.4. in greenhouse studies, swiss chard (beta vulgaris var. cicla), a cd-acc ...201525174422
anti-aging potential of phytoextract loaded-pharmaceutical creams for human skin cell longetivity.the exposure to ultraviolet radiations (uvr) is the key source of skin sunburn; it may produce harmful entities, reactive oxygen species (ros), leading to aging. the skin can be treated and protected from the injurious effects of ros by using various pharmaceutical formulations, such as cream. cream can be loaded with antioxidants to quench ros leading to photo-protective effects. moreover, modern medicines depend on ethnobotanicals for protection or treatment of human diseases. this review arti ...201526448818
simple and efficient way to detect small polymorphic bands in plants.there are many ways to detect polymorphism. in this study we use the microsatellite markers to detect the polymorphism for the salt tolerance. this method has been successfully conducted in oryza sativa and brassica juncea. the results are reproducible. in contrast to previous methods, our method is simple and quite accurate for detecting the polymorphic bands. in this study instead of using agarose gel and ethidium bromide staining, we used non-denaturing polyacrylamide gel and a low-cost impro ...201526484259
genomic selection and association mapping in rice (oryza sativa): effect of trait genetic architecture, training population composition, marker number and statistical model on accuracy of rice genomic selection in elite, tropical rice breeding lines.genomic selection (gs) is a new breeding method in which genome-wide markers are used to predict the breeding value of individuals in a breeding population. gs has been shown to improve breeding efficiency in dairy cattle and several crop plant species, and here we evaluate for the first time its efficacy for breeding inbred lines of rice. we performed a genome-wide association study (gwas) in conjunction with five-fold gs cross-validation on a population of 363 elite breeding lines from the int ...201525689273
analysis of aliphatic waxes associated with root periderm or exodermis from eleven plant species.aliphatic waxes can be found in association with suberized tissues, including roots. non-polar lipids were isolated by rapid solvent extraction of mature regions of intact roots from eleven angiosperms, including both monocots and dicots. the majority of roots analyzed were taproots or tuberous taproots that had undergone secondary growth and thus were covered by a suberized periderm. the exceptions therein were maize (zea mays l.) and rice (oryza sativa l.), which present a suberized exodermis. ...201526143051
effects of winter covering crop residue incorporation on ch₄ and n₂o emission from double-cropped paddy fields in southern china.residue management in cropping systems is useful to improve soil quality. however, the studies on the effects of residue management on methane (ch4) and nitrous oxide (n2o) emission from paddy field in southern china are few. therefore, the emissions of ch4 and n2o were investigated in double cropping rice (oryza sativa l.) systems with different winter covering crops using the static chamber-gas chromatography technique to assess the effects of different covering crops on the emissions of green ...201525913315
the rice transcription factor wrky53 suppresses herbivore-induced defenses by acting as a negative feedback modulator of mitogen-activated protein kinase activity.the mechanisms by which herbivore-attacked plants activate their defenses are well studied. by contrast, little is known about the regulatory mechanisms that allow them to control their defensive investment and avoid a defensive overshoot. we characterized a rice (oryza sativa) wrky gene, oswrky53, whose expression is rapidly induced upon wounding and induced in a delayed fashion upon attack by the striped stem borer (ssb) chilo suppressalis. the transcript levels of oswrky53 are independent of ...201526453434
characterization of competitive interactions in the coexistence of bt-transgenic and conventional rice.transgene flow through pollen and seeds leads to transgenic volunteers and feral populations in the nature, and consumer choice and economic incentives determine whether transgenic crops will be cultivated in the field. transgenic and non-transgenic plants are likely to coexist in the field and natural habitats, but their competitive interactions are not well understood.201525928331
how to isolate a plant's hypomethylome in one shot.genome assembly remains a challenge for large and/or complex plant genomes due to their abundant repetitive regions resulting in studies focusing on gene space instead of the whole genome. thus, dna enrichment strategies facilitate the assembly by increasing the coverage and simultaneously reducing the complexity of the whole genome. in this paper we provide an easy, fast, and cost-effective variant of mre-seq to obtain a plant's hypomethylome by an optimized methyl filtration protocol followed ...201526421293
barnyard grasses were processed with rice around 10000 years ago.rice (oryza sativa) is regarded as the only grass that was selected for cultivation and eventual domestication in the yangtze basin of china. although both macro-fossils and micro-fossils of rice have been recovered from the early neolithic site of shangshan, dating to more than 10,000 years before present (bp), we report evidence of phytolith and starch microfossils taken from stone tools, both for grinding and cutting, and cultural layers, that indicating barnyard grass (echinochloa spp.) was ...201526536839
competition between rice (oryza sativa l.) and (barnyardgrass (echinochloa crus-galli (l.) p. beauv.) as affected by methanol foliar application.pot experiment was conducted in iran, to evaluate the effect of methanol on competition between rice (oryza sativa) and barnyardgrass (echinochloa crus-galli). the experiment was conducted as a randomized complete block design with a factorial treatment arrangement and three replicates. factors were two aqueous methanol foliar applications (0, and 14% v/v) and five rice: barnyardgrass ratios (100:0, 75:25, 50:50, 25:6, and 0:100). replacement series diagrams for aboveground dry weight illustrate ...201526062115
molecular characterization and in silico analysis of sorghum panallergens: profilin and polcalin.in india, 20-30% of the human population suffer from allergic rhinitis and 15% of them develop asthma. plant pollens are one of the causative aeroallergens and are mixture of a number of molecules including major and minor allergens (panallergens). profilin and polcalcin are the known pollen specific panallergens. allergenicity of the sorghum plant in andhra pradesh was found to be 54.9%. but the allergens responsible have not been characterized well. this study highlights identification and mol ...201526669015
characterization of a novel clade of xanthomonas isolated from rice leaves in mali and proposal of xanthomonas maliensis sp. nov.four bacterial strains, designated m89, m92, m97(t), and m106, were isolated in a previous study from surface-sterilized leaves of rice (oryza sativa) or murainagrass (ischaemum rugosum) at three sites in mali, africa. here they were examined by a polyphasic taxonomic approach and analysis of a whole-genome sequence. phylogenetic analyses based on 16s rrna sequence and multilocus sequence analysis of seven genes showed that these four strains formed a distinct lineage representing a novel specie ...201525588569
streptomyces oryzae sp. nov., an endophytic actinomycete isolated from stems of rice plant.an actinomycete strain s16-07(t), isolated from surface-sterilized stems of rice plant (oryza sativa l.), was characterized using a polyphasic approach. phylogenetic analysis of 16s rrna gene sequences indicated affiliation of the strain belonged to the genus streptomyces. the highest levels of sequence similarity were found with streptomyces smyrnaeus sm3501(t) (97.7% similarity), s. abikoensis nbrc 13860(t) (97.6% similarity) and s. thermocarboxydovorans nbrc 16324(t) (97.5% similarity). the c ...201525586021
growth enhancement of rice (oryza sativa) by phosphate solubilizing gluconacetobacter sp. (mtcc 8368) and burkholderia sp. (mtcc 8369) under greenhouse conditions.two indigenous rhizospheric phosphate solubilizing isolates psb 12 identified as gluconacetobacter sp. (mtcc 8368) and psb 73 identified as burkholderia sp. (mtcc 8369) were examined for their growth enhancement potential of rice (jyothi ptb 39) under pot culture assays. the results showed significant impact on microbial count and psb population, phosphatase and dehydrogenase activity, available phosphorous in the soil, plant nutrient uptake and yield parameters. gluconacetobacter sp. + rp60 tre ...201528324538
roseomonas oryzicola sp. nov., isolated from the rhizosphere of rice (oryza sativa l.).a gram-stain-negative, coccobacilli-shaped bacterium, designated yc6724t, was isolated from the rhizosphere of rice in jinju, korea. the taxonomy of strain yc6724t was studied using a polyphasic approach. strain yc6724t grew optimally at 30 °c and ph 7.0-8.0. comparative 16s rrna gene sequence analyses showed that the strain was most closely related to roseomonas soli 5n26t (98.4 % 16s rrna gene sequence similarity), roseomonas lacus thg33t(97.3 %) and roseomonas terrae ds-48t (97.3 %). sequence ...201526443678
flectobacillus rhizosphaerae sp. nov., isolated from the rhizosphere soil of oryza sativa (l.), and emended description of the genus flectobacillus.a gram-stain-negative, aerobic, pale orange, rod-shaped, non-motile bacterial strain, designated jc289t was isolated from a rhizosphere soil sample of the plant oryza sativa (l.). 16s rrna gene sequence analysis clearly allocated strain jc289t to the flectobacillus cluster, showing highest sequence similarities to flectobacillus roseus gfa-11t (99.5 %), flectobacillus major atcc 29496t (96.7 %) and flectobacillus lacus cl-gp79t (94.6 %). major (>5 %) fatty acids of strain jc289t were c16 : 1ω5c, ...201526297007
the lasioseius phytoseioides species group (acari: blattisociidae): new characterisation, description of a new species, complementary notes on seven described species and a taxonomic key for the group.the lasioseius phytoseioides species group was first characterised over 50 years ago. two species of this group, lasioseius chaudhrii (wu & wang) and lasioseius parberlesei bhattacharyya, have been considered potentially effective as biological control agents of pest mites of the family tarsonemidae on rice (oryza sativa l.) in asia. a new characterisation of the species group is presented, taking into account a new species here described, lasioseius piracicabensis moraes & pérez-madruga n. sp., ...201526249936
micromonospora oryzae sp. nov., isolated from roots of upland rice.an actinomycete strain designated as cp2r9-1t was isolated from root internal tissues of upland rice (oryza sativa). based on the polyphasic approach, strain cp2r9-1t was characterized as a member of the genus micromonospora. meso-diaminopimelic acid and 3-oh-diaminopimelic acid were present in the cell-wall peptidoglycan. the polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannosides, two unidentified phospholi ...201526238346
mapping of a cellulose-deficient mutant named dwarf1-1 in sorghum bicolor to the green revolution gene gibberellin20-oxidase reveals a positive regulatory association between gibberellin and cellulose biosynthesis.here, we show a mechanism for expansion regulation through mutations in the green revolution gene gibberellin20 (ga20)-oxidase and show that gas control biosynthesis of the plants main structural polymer cellulose. within a 12,000 mutagenized sorghum bicolor plant population, we identified a single cellulose-deficient and male gametophyte-dysfunctional mutant named dwarf1-1 (dwf1-1). through the sorghum propinquum male/dwf1-1 female f2 population, we mapped dwf1-1 to a frameshift in ga20-oxidase ...201526198258
a natural rice rhizospheric bacterium abates arsenic accumulation in rice (oryza sativa l.).a natural rice rhizospheric isolate abates arsenic uptake in rice by increasing fe plaque formation on rice roots. rice (oryza sativa l.) is the staple food for over half of the world's population, but its quality and yield are impacted by arsenic (as) in some regions of the world. bacterial inoculants may be able to mitigate the negative impacts of arsenic assimilation in rice, and we identified a nonpathogenic, naturally occurring rice rhizospheric bacterium that decreases as accumulation in r ...201526059607
micromonospora endophytica sp. nov., an endophytic actinobacteria of thai upland rice (oryza sativa).an actinobacterial strain, dcwr9-8-2(t), was isolated from a leaf of thai upland rice (oryza sativa) collected in chumporn province, thailand. strain dcwr9-8-2(t) is gram-stain-positive aerobic bacteria that produce single spores directly on the vegetative hypha. cell wall peptidoglycan of this strain exhibits meso-diaminopimelic acid and glycine, the reducing sugars of whole-cell hydrolysate are arabinose, glucose, ribose, xylose and small amount of mannose. the phospholipid profiles in the mem ...201525966850
coapplication of chicken litter biochar and urea only to improve nutrients use efficiency and yield of oryza sativa l. cultivation on a tropical acid soil.the excessive use of nitrogen (n) fertilizers in sustaining high rice yields due to n dynamics in tropical acid soils not only is economically unsustainable but also causes environmental pollution. the objective of this study was to coapply biochar and urea to improve soil chemical properties and productivity of rice. biochar (5 t ha(-1)) and different rates of urea (100%, 75%, 50%, 25%, and 0% of recommended n application) were evaluated in both pot and field trials. selected soil chemical prop ...201526273698
chemiluminescence during rice seed imbibition at different temperatures.the chemiluminescence (cl) of rice (oryza sativa l.) seeds at different temperatures and the cl spectra of rice seed, caryopses and seed coat were studied during early imbibition. compared with the cl of barley (hordeum vulgare l.) and soybean (glycine max l. merr.) seeds, the cl of rice seeds had a non-linear, logarithmic-like increase of intensity in the temperature range 30-50 degrees c. the van't hoff coefficient q(10) = i(t+10)/i(t) was equal to 2. the emission spectrum of whole rice seed, ...201516100741
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