Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| aggregatibacter actinomycetemcomitans growth in biofilm versus planktonic state: differential expression of proteins. | aggregatibacter actinomycetemcomitans (aa) is a pathogenic bacterium residing in the subgingival plaque biofilm strongly associated with the pathogenesis of periodontitis. the aim of this investigation was to study the protein differential expression of aa when growing on biofilm compared with planktonic state using proteomic analysis by the 2d-dige system. eighty-seven proteins were differentially expressed during biofilm growth (1.5-fold, p < 0.05), with 13 overexpressed and 37 down-expressed. ... | 2017 | 28707473 |
| the association of periodontopathic bacteria levels in saliva and tongue coating with oral malodor in periodontitis patients. | to elucidate the association oral malodor with the levels of periodontopathic bacteria in saliva and tongue coating of periodontitis patients with oral malodor. | 2017 | 28674708 |
| chronic periodontal disease, periodontal pathogen colonization, and an increased risk of precancerous gastric lesions. | the present study assessed the association between periodontal pathogen colonization and the potential risk of developing precancerous lesions of gastric cancer (plgc) in a clinical setting. | 2017 | 28671506 |
| aggregatibacter actinomycetemcomitans serotype prevalence and antibiotic resistance in a uk population with periodontitis. | aggregatibacter actinomycetemcomitans is a recognised pathogen involved in aggressive periodontitis. seven serotypes of a. actinomycetemcomitans exist with a range of virulence and distribution dependent on ethnicity and geography. the ability of a. actinomycetemcomitans to invade soft tissue can necessitate the use of systemic antibiotics for treatment, however variations in its antibiotic susceptibility exist dependent on geographical location. | 2017 | 28668698 |
| characterization and serotype distribution of aggregatibacter actinomycetemcomitans: relationship of serotypes to herpesvirus and periodontal status in indian subjects. | the virulence of aggregatibacter actinomycetemcomitans (a. actinomycetemcomitans) in any individual depends on the type of strain of this bacterium. to our knowledge, there have been no studies reported in indian subjects about a. actinomycetemcomitans serotype occurrence, co-existence with herpes virus and the possible influence of such co-existence on periodontal pathology. | 2017 | 28668607 |
| effects of photodynamic therapy in periodontal treatment: a randomized, controlled clinical trial. | to evaluate the effects of photodynamic therapy (pdt) in the nonsurgical treatment of chronic periodontitis. | 2017 | 28667678 |
| natural monoclonal antibody to oxidized low-density lipoprotein and aggregatibacter actinomycetemcomitans. | natural antibodies are produced by b lymphocytes without exogenous antigenic exposure and are present at the time of birth. they usually bind to conserved epitopes on antigens of different chemical compositions. we cloned and characterized a natural mouse monoclonal igm antibody (aa_mab) by selecting the binding to malondialdehyde acetaldehyde (maa) adducts on low-density lipoprotein (ldl). the data showed that the aa_mab cross-reacted with aggregatibacter actinomycetemcomitans (aa) bacteria, an ... | 2017 | 28667536 |
| stimulatory effect of aggregatibacter actinomycetemcomitans dna on proinflammatory cytokine expression by human gingival fibroblasts. | while different virulence factors have been reported of aggregatibacter actinomycetemcomitans (aa), there is little information about the stimulatory effect of its dna. the main purpose of this study was to assess the inflammatory response of human gingival fibroblasts (hgfs) stimulated with a. actinomycetemcomitans dna. | 2017 | 28641178 |
| is aggregatibacter actinomycetemcomitans the missing link between periodontitis and rheumatoid arthritis? | 2017 | 28632321 | |
| lactobacillus salivarius nk02: a potent probiotic for clinical application in mouthwash. | a specific strain of naturally occurring oral lactobacilli was isolated and identified based on morphological, biochemical, and 16s rrna gene sequencing. the phylogenetic affiliation of the isolate confirmed that the nk02 strain had close association with the lactobacillus salivarius. an effective mouthwash was developed for treatment of periodontitis and suppression of the indicator bacterium aggregatibacter actinomycetemcomitans which is an obvious pathogen of periodontal disease. the mouthwas ... | 2017 | 28631250 |
| mikania glomerata sprengel extract and its major compound ent-kaurenoic acid display activity against bacteria present in endodontic infections. | the search for new, effective and safe antimicrobial compounds from plant sources has continued to play an important role in the maintenance of human health since ancient times. such compounds can be used to help to eradicate microorganisms from the root canal system, preventing/healing periapical diseases. mikania glomerata (spreng.), commonly known as "guaco," is a native climbing plant from brazil that displays a wide range of pharmacological properties. many of its activities have been attri ... | 2017 | 28627377 |
| aggregatibacter actinomycetemcomitans regulates the expression of integrins and reduces cell adhesion via integrin α5 in human gingival epithelial cells. | gingival epithelial cells form a physiological barrier against bacterial invasion. excessive bacterial invasion destroys the attachment between the tooth surface and the epithelium, resulting in periodontitis. integrins play a significant role in cell attachment; therefore, we hypothesized that bacterial infection might decrease the expressions of these integrins in gingival epithelial cells, resulting in reduced cell adhesion. immortalized human gingival epithelial cells were co-cultured with a ... | 2017 | 28593565 |
| the microbial changes in subgingival plaques of orthodontic patients: a systematic review and meta-analysis of clinical trials. | orthodontic treatment was found to have an impact on the quantity and constitution of subgingival microbiota. however, contradictory findings regarding the effects of fixed appliances on microbial changes were reported. the aim of this systematic review was to investigate the microbial changes in subgingival plaques of orthodontic patients. | 2017 | 28576147 |
| ag nanoparticle-coated zirconia for antibacterial prosthesis. | bacterial adhesion to dental materials is a major cause of caries and periodontitis, necessitating the development of compounds such as yttria-stabilized zirconia (ysz) and silver nanoparticles (agnps), which are widely employed in medicine due to their high antimicrobial activity and low cytotoxicity. the main goal of this study is the synthesis of the broad antimicrobial activity of agnp-coated ysz with facile methods. the bactericidal agnps were immobilized on the surface of ysz and tested fo ... | 2017 | 28575940 |
| the role of 5-lipoxygenase in aggregatibacter actinomycetemcomitans-induced alveolar bone loss. | leukotrienes (lts) are pro-inflammatory lipid mediators formed by the enzyme 5-lipoxygenase (5-lo). the involvement of 5-lo metabolites in periodontal disease (pd) is not well defined. this study aimed to assess the role of 5-lo in experimental pd induced by aggregatibacter actinomycetemcomitans (aa). | 2017 | 28569991 |
| role of de-n-acetylase pgab from aggregatibacter actinomycetemcomitans in exopolysaccharide export in biofilm mode of growth. | aggregatibacter actinomycetemcomitans, a gram-negative bacterium, is the causative agent of localized aggressive periodontitis. attachment to a biotic surface is a critical first step in the a. actinomycetemcomitans infection process for which exopolysaccharides have been shown to be essential. in addition, the pga operon, containing genes encoding for biosynthetic proteins for poly-n-acetyl glucosamine (pnag), plays a key role in a. actinomycetemcomitans virulence, as a mutant strain lacking th ... | 2017 | 28548373 |
| cross-reactive saliva iga antibodies to oxidized ldl and periodontal pathogens in humans. | oxidized low-density lipoproteins (oxldl) are formed as a result of lipid peroxidation and are highly immunogenic and proatherogenic. in this study, saliva antibodies binding to oxldl, porphyromonas gingivalis (pg) and aggregatibacter actinomycetemcomitans (aa) were characterized and their cross-reactivity was evaluated. | 2017 | 28548243 |
| the in vivo t helper type 17 and regulatory t cell immune responses to aggregatibacter actinomycetemcomitans. | the periodontal pathogen aggregatibacter actinomycetemcomitans is known to elicit a systemic immune response in the infected host, and occasionally causes non-oral infections. detailed information on its immunopathological responses and the involvement of bacterial virulence factors remains to be elucidated. the aim of this study was to assess the systemic immune response to a. actinomycetemcomitans oral infection. we used an animal model that simulates systemic dissemination of the bacteria by ... | 2017 | 28544588 |
| herpes simplex 1 and periopathogen role in peri-implantitis. | the objectives of this study were to compare the qualitative and quantitative profiles of herpes simplex virus type i (hsv-1) in implant surfaces between participants with peri-implantitis (pi) and healthy peri-implant tissues and to quantitatively assess the relation between hsv-1 and periopathogens inside the microbiological profile associated with pi. | 2017 | 28512280 |
| increased leukotoxin production: characterization of 100 base pairs within the 530 base pair leukotoxin promoter region of aggregatibacter actinomycetemcomitans. | aggregatibacter actinomycetemcomitans leukotoxin (ltxa) is a major virulence factor that kills leukocytes permitting it's escape from host immune surveillance. a. actinomycetemcomitans strains can produce high or low levels of toxin. genetic differences reside in the "so called jp2" ltxa promoter region. these hyper-leukotoxin producing strains with the 530 bp deletion have been studied in detail. however, regions contained within the 530 bp deletion that could be responsible for modulation of l ... | 2017 | 28507341 |
| putative periodontal pathogens in the subgingival plaque of sudanese subjects with aggressive periodontitis. | there has been limited study of the bacterial species associated with aggressive periodontitis (agp) in high-risk populations in africa. the aim of this study was to investigate and quantify the presence of four putative periodontal pathogens in the subgingival plaque of sudanese subjects with agp. a secondary aim was to investigate the effect of varying the detection threshold on the reported prevalence of the bacterial species investigated. | 2017 | 28499236 |
| investigations of a possible chemical effect of salvadora persica chewing sticks. | salvadora persica is commonly used chewing sticks in many parts of the world as an oral hygiene tool. this study measured the amount of benzyl isothiocyanate (bitc) released into the mouth and assessed its retention time in saliva. the study also tested if the released amount of bitc could potentially be antibacterial or cytotoxic. twelve subjects brushed their teeth with fresh miswak once, twice, and four times. the amount of bitc in the saliva and in the used brushes was quantified using gas c ... | 2017 | 28484501 |
| some gram-negative lipoproteins keep their surface topology when transplanted from one species to another and deliver foreign polypeptides to the bacterial surface. | in gram-negative bacteria, outer membrane-associated lipoproteins can either face the periplasm or protrude out of the bacterial surface. the mechanisms involved in lipoprotein transport through the outer membrane are not fully elucidated. some lipoproteins reach the surface by using species-specific transport machinery. by contrast, a still poorly characterized group of lipoproteins appears to always cross the outer membrane, even when transplanted from one organism to another. to investigate s ... | 2017 | 28483926 |
| defining genetic fitness determinants and creating genomic resources for an oral pathogen. | periodontitis is a microbial infection that destroys the structures that support the teeth. although it is typically a chronic condition, rapidly progressing, aggressive forms are associated with the oral pathogen aggregatibacter actinomycetemcomitans one of this bacterium's key virulence traits is its ability to attach to surfaces and form robust biofilms that resist killing by the host and antibiotics. though much has been learned about a. actinomycetemcomitans since its initial discovery, we ... | 2017 | 28476775 |
| monitoring gene expression of rcpa from aggregatibacter actinomycetemcomitans versus antimicrobial photodynamic therapy by relative quantitative real-time pcr. | aggregatibacter actinomycetemcomitans is an important pathogen that is frequently found in various infections, particularly aggressive periodontitis. in this study, we described the outcome of the expression level of a. actinomycetemcomitans virulence factor following treatment by antimicrobial photodynamic therapy (apdt) with indocyanine green (icg) as a photosensitizing agent. | 2017 | 28450261 |
| antimicrobial photodynamic activity and cytocompatibility of au25(capt)18 clusters photoexcited by blue led light irradiation. | antimicrobial photodynamic therapy (apdt) has beneficial effects in dental treatment. we applied captopril-protected gold (au25(capt)18) clusters as a novel photosensitizer for apdt. photoexcited au clusters under light irradiation generated singlet oxygen ((1)o2). accordingly, the antimicrobial and cytotoxic effects of au25(capt)18 clusters under dental blue light-emitting diode (led) irradiation were evaluated. (1)o2 generation of au25(capt)18 clusters under blue led irradiation (420-460 nm) w ... | 2017 | 28435253 |
| aggregatibacter actinomycetemcomitans pneumonia with chest and abdominal wall involvement. | a 54-year-old man presented with a productive cough, chest pain, fever and weight loss. initial analysis revealed a palpable chest wall mass and consolidation in the left lower lobe and pleural abnormalities on imaging. at that point no infectious cause or malignancy was identified. microbiological analysis of a needle biopsy from a newly developed abdominal wall mass revealed growth of aggregatibacter actinomycetemcomitans the patient was successfully treated with antibiotic therapy for 1 year. ... | 2017 | 28432161 |
| photo-activated elimination of aggregatibacter actinomycetemcomitans in planktonic culture: comparison of photodynamic therapy versus photothermal therapy method. | periodontal pathogens are the main factors responsible for periodontal diseases and considering the limitations of conventional mechanical debridement, new treatment approaches are under investigation. this study was designed to evaluate and compare the antibacterial effects of two different systems of photodynamic and photothermal therapy on aggregatibacter actinomycetemcomitans as the main pathogen involved in aggressive periodontitis. | 2017 | 28414081 |
| in vitro antimicrobial activity of thymus vulgaris essential oil against major oral pathogens. | the objective of present investigation was to determine antimicrobial activity of thymus vulgaris oil on some oral pathogens. thymus vulgaris oil was prepared by hydrodistillation and tested against 30 clinical isolates of each of streptococcus pyogenes, streptococcus mutans, candida albicans, porphyromonas gingivalis, and aggregatibacter actinomycetemcomitans, prepared from related oral infections using agar disk diffusion and broth microdilution methods. thymus vulgaris oil at concentrations o ... | 2017 | 28397552 |
| the cage gene sequence as a diagnostic marker to identify jp2 and non-jp2 highly leukotoxic aggregatibacter actinomycetemcomitans serotype b strains. | aggregatibacter actinomycetemcomitans is involved in oral and systemic infections, and is associated with, eg aggressive forms of periodontitis and with endocarditis. the cage gene encodes a ≈39 kda putative exotoxin expressed by a. actinomycetemcomitans. the level of conservation of cage, and its possible significance in periodontal disease, has not yet been thoroughly investigated. in the present study, the role of the cage gene as a diagnostic marker has been investigated. | 2017 | 28397250 |
| porphyromonas gingivalis antibody levels and diagnosis of coronary artery disease in hiv-positive individuals. | periodontal disease has been associated with cardiovascular disease in the general population. it is unknown whether igg antibody levels for periodontal pathogens are associated with the diagnosis of coronary artery disease (cad) in hiv-positive individuals. | 2017 | 28397248 |
| soluble epoxide hydrolase pharmacological inhibition decreases alveolar bone loss by modulating host inflammatory response, rank-related signaling, endoplasmic reticulum stress, and apoptosis. | epoxyeicosatrienoic acids (eets), metabolites of arachidonic acid derived from the cytochrome p450 enzymes, are mainly metabolized by soluble epoxide hydrolase (seh) to their corresponding diols. eets but not their diols, have anti-inflammatory properties, and inhibition of seh might provide protective effects against inflammatory bone loss. thus, in the present study, we tested the selective seh inhibitor, 1-trifluoromethoxyphenyl-3-(1-propionylpiperidin-4-yl) urea (tppu), in a mouse model of p ... | 2017 | 28356494 |
| antimicrobial effects of mastic extract against oral and periodontal pathogens. | various antimicrobial agents are widely used in the therapy of oral inflammatory diseases. however, their side effects and the appearance of drug resistance justify research on natural antimicrobial agents to target oral pathogens that are safe for the host. in the present study, antimicrobial properties of mastic extract on commensal and pathogenic oral bacteria, as well as its possible cytotoxic effect toward cells of epithelial and mesenchymal origin, were evaluated and compared with the comm ... | 2017 | 28067105 |
| assessing the progression of chronic periodontitis using subgingival pathogen levels: a 24-month prospective multicenter cohort study. | the diagnosis of the progression of periodontitis presently depends on the use of clinical symptoms (such as attachment loss) and radiographic imaging. the aim of the multicenter study described here was to evaluate the diagnostic use of the bacterial content of subgingival plaque recovered from the deepest pockets in assessing disease progression in chronic periodontitis patients. | 2017 | 28093069 |
| comparison of two different sampling methods for subgingival plaque: subgingival paper points or mouthrinse sample? | the collection of subgingival plaque samples with paper points is time-consuming and accident-sensitive. however, the collection of saliva is simple and contains pathogens of all intraoral surfaces. the aim of this study is to investigate whether a sampling strategy with mouthrinse (mouthrinse sample [msp]; test) leads to results comparable with standard sampling method (multiple site test from the deepest pocket of each quadrant [mt4]; control). | 2017 | 27786617 |
| nanofibers containing tetracycline/β-cyclodextrin: physico-chemical characterization and antimicrobial evaluation. | this study aimed to compare two nanofiber drug delivery systems that were prepared with an electrospun process and have the potential to serve as adjuvants for the treatment of periodontal disease. the first system was composed of polycaprolactone loaded with tetracycline (tcn) and the second was composed of polycaprolactone loaded with tetracycline/β-cyclodextrin (tcn:bcd). an antimicrobial diffusion test was performed for each of these sets of nanofibers with the microorganisms, aggregatibacte ... | 2017 | 27842841 |
| nadph oxidase contributes to resistance against aggregatibacter actinomycetemcomitans-induced periodontitis in mice. | aggregatibacter actinomycetemcomitans is a gram-negative commensal bacterium of the oral cavity which has been associated with the pathogenesis of periodontitis with severe alveolar bone destruction. the role of host factors such as reactive oxygen and nitrogen intermediates in periodontal a. actinomycetemcomitans infection and progression to periodontitis is still ill-defined. therefore, this study aimed to analyze the role of nadph oxidase and inducible nitric oxide synthase (inos) in a murine ... | 2017 | 27849181 |
| detection and quantification of aggregatibacter actinomycetemcomitans, porphyromonas gingivalis and streptococcus oralis in blood samples with different microbiological identification methods: an in vitro study. | culture-based methods (culture broth bottles or lysis methods) have been the standard for detecting bacteremia. more recently, quantitative polymerase chain reaction (qpcr) was proposed as a more sensitive and specific test although none of them has been validated for the identification of periodontal pathogens (fastidious growing bacteria) in blood samples. | 2017 | 27875793 |
| high-risk periodontal pathogens contribute to the pathogenesis of atherosclerosis. | periodontal disease (pd) is generated by microorganisms. these microbes can enter the general circulation causing a bacteraemia. the result can be adverse systemic effects, which could promote conditions such as cardiovascular disease. level a evidence supports that pd is independently associated with arterial disease. pd is a common chronic condition affecting the majority of americans 30 years of age and older. atherosclerosis remains the largest cause of death and disability. studies indicate ... | 2017 | 27899684 |
| thermoreversible gel for intrapocket delivery of green tea catechin as a local drug delivery system: an original research. | the periodontal therapies along with systemic antibiotic therapy aim at eliminating the subgingival microbiota to arrest the progression of periodontal diseases. the complete elimination is often difficult, and thus the probability of repopulation after periodontal therapy is also high. the objectives of the study are to develop in situ thermoreversible gelling system of green tea catechins suitable for periodontal pocket administration, which would act as an adjunct to mechanical periodontal th ... | 2017 | 27833893 |
| periodontitis and myocardial hypertrophy. | there is a deep relationship between cardiovascular disease and periodontitis. it has been reported that myocardial hypertrophy may be affected by periodontitis in clinical settings. although these clinical observations had some study limitations, they strongly suggest a direct association between severity of periodontitis and left ventricular hypertrophy. however, the detailed mechanisms between myocardial hypertrophy and periodontitis have not yet been elucidated. recently, we demonstrated tha ... | 2017 | 27829660 |
| influence of culture conditions on porphyrin production in aggregatibacter actinomycetemcomitans and porphyromonas gingivalis. | increasing antibiotic resistance among pathogens has raised the demands for new treatment methods such as antimicrobial photodynamic therapy (apdt) and phototherapy (pt). experiments for investigating the effects of these methods are often performed in vitro, but the procedures for cultivation of microbes vary between different studies. the aim of this study has been to elucidate how the profile of endogenously produced porphyrins differs by changing the variables of bacteria culturing condition ... | 2017 | 27825899 |
| enhanced antimicrobial activities of silver-nanoparticle-decorated reduced graphene nanocomposites against oral pathogens. | as a means of capitalizing on the synergistic properties between reduced graphene nanosheets (r-gns) and silver nanoparticles (agnps), an efficient and convenient chemical reduction method was used to prepare silver-nanoparticle-decorated reduced graphene nanocomposites (r-gns/ag). the products were characterized by scanning electron microscopy (sem), transmission electron microscopy (tem), x-ray diffraction (xrd), and raman spectroscopy, which confirmed the loading of well-dispersed silver nano ... | 2017 | 27987652 |
| rt-pcr quantification of periodontal pathogens in crack users and non-users. | to compare counts of aggregatibacter actinomycetemcomitans, prevotella intermedia, porphyromonas gingivalis and fusobacterium nucleatum between crack users and non-users. | 2017 | 27982490 |
| comparison of nonsurgical periodontal therapy with oral hygiene instruction alone for chronic periodontitis. | we evaluated changes in clinical variables and microbiological profiles of periodontopathogens among 56 patients with moderate to severe cp who were randomly assigned to oral hygiene instruction (ohi; n = 28) or nonsurgical periodontal treatment (nspt; n = 28). periodontal variables were assessed and subgingival plaque samples were obtained from deep pockets (≥5 mm) at baseline and 3 months after treatment. real-time polymerase chain reaction was used to quantify actinobacillus actinomycetemcomi ... | 2017 | 28367891 |
| association between specific periodontal pathogens, toll-like receptor-4, and nuclear factor-κb expression in placental tissues of pre-eclamptic women with periodontitis. | the aim of the present study was to determine the association between the presence of specific periodontal pathogens, toll-like receptor-4 (tlr-4), and nuclear factor-κb (nf-κb) expression in the placental tissues of pre-eclamptic women. | 2017 | 28349674 |
| compositional differences in multi-species biofilms formed on various orthodontic adhesives. | to investigate the aspects of multi-species biofilm formation on various orthodontic adhesives with different surface characteristics. | 2017 | 28339597 |
| reproducibility of a commercially available subgingival plaque sampling strategy and analysis strategy with oligonucleotide probes. | the aim was to evaluate the intra-test agreement of pooled samples from the deepest periodontal pocket of each quadrant with a commercially available test kit based on hybridization of 16s rrna. | 2017 | 28325127 |
| tio2 nanorod arrays as a photocatalytic coating enhanced antifungal and antibacterial efficiency of ti substrates. | to investigate the photocatalytic inactivation of fungi and bacteria mediated by tio2 nanorod arrays (tnrs). | 2017 | 28322109 |
| quorum sensing of streptococcus mutans is activated by aggregatibacter actinomycetemcomitans and by the periodontal microbiome. | the oral cavity is inhabited by complex microbial communities forming biofilms that can cause caries and periodontitis. cell-cell communication might play an important role in modulating the physiologies of individual species, but evidence so far is limited. | 2017 | 28320314 |
| subgingival microbiota in caucasian patients with desquamative gingivitis: a cross-sectional study. | the presence of epithelial desquamation, erythema, and erosions on the gingival tissue is usually described in literature as desquamative gingivitis (dg). a wide range of autoimmune/dermatological disorders can manifest as dg, although the two more common are oral lichen planus and mucous membrane pemphigoid. the aim of this study was to investigate the prevalence of 11 periodonto-pathogenic microorganisms in dg cases and to compare it with microbiologic status of individuals affected by plaque- ... | 2017 | 28304209 |
| aggregatibacter actinomycetemcomitans osteomyelitis in a 12 year old boy: case report emphasizing the importance of tissue culture, and review of literature. | aggregatibacter actinomycetemcomitans most commonly causes periodontitis but has been reported to infect heart valves, soft tissue, brain and lungs, and distal bones. osteomyelitis distal to the jaw is rarely described. | 2017 | 28288638 |
| efficacy of natural and allopathic antimicrobial agents incorporated onto guided tissue regeneration membrane against periodontal pathogens: an in vitro study. | periodontal disease is one of the most prevalent afflictions worldwide. it is an infection of the periodontium as a result of subgingival colonization of the specific microbiota, leading to loss of attachment, which requires optimal care for regeneration to its pre-disease state. guided tissue regeneration (gtr) is one of the successful treatment modalities in periodontal regenerative therapy, but is vulnerable to bacterial colonization. the conflict between usage of classical antibiotics and pl ... | 2017 | 28274052 |
| oral microbe-host interactions: influence of β-glucans on gene expression of inflammatory cytokines and metabolome profile. | the aim of this study was to evaluate the effects of β-glucan on the expression of inflammatory mediators and metabolomic profile of oral cells [keratinocytes (oba-9) and fibroblasts (hgf-1) in a dual-chamber model] infected by aggregatibacter actinomycetemcomitans. the periodontopathogen was applied and allowed to cross the top layer of cells (oba-9) to reach the bottom layer of cells (hgf-1) and induce the synthesis of immune factors and cytokines in the host cells. β-glucan (10 μg/ml or 20 μg ... | 2017 | 28270109 |
| the mechanism of jurkat cells apoptosis induced by aggregatibacter actinomycetemcomitans cytolethal distending toxin. | cytolethal distending toxin (cdt) which is produced by aggregatibacter actinomycetemcomitans causes apoptosis in lymphocytes. but the specific mechanism is not clear. the aim of our research was to investigate the effect and mechanism during this process. the wild-type cdta, cdtb, cdtc (cdta(w), cdtb(w), cdtc(w)) and mutant cdtb (cdtb(m)) were expressed and purified respectively and the purity of each subunit was examined by bandscan software. and the type i deoxyribonuclease and pi-3,4,5-tripho ... | 2017 | 28247205 |
| response to antiseptic agents of periodontal pathogens in in vitro biofilms on titanium and zirconium surfaces. | the aim of this study was to develop in vitro biofilms on sla titanium (ti-sla) and zirconium oxide (zro2) surfaces and to evaluate the effect of antiseptic agents on the number of putative periodontal pathogenic species. | 2017 | 28237092 |
| real-time quantitative reverse transcription-pcr analysis of expression stability of aggregatibacter actinomycetemcomitans fimbria-associated gene in response to photodynamic therapy. | aggregatibacter actinomycetemcomitans is an etiological agent of both chronic and aggressive periodontitis. dissemination of a. actinomycetemcomitans from the oral cavity and initiation of systemic infections has led to new approaches for treatment being needed. in this study, a series of experiments presented investigated the effect of methylene blue (mb)-mediated antimicrobial photodynamic therapy (apdt) on cell viability and expression of fimbria-associated gene (rcpa) in a. actinomycetemcomi ... | 2017 | 28216095 |
| differential human th22-lymphocyte response triggered by aggregatibacter actinomycetemcomitans serotypes. | in aggregatibacter actinomycetemcomitans, different serotypes have been described based on lipopolysaccharide (lps) antigenicity. when t lymphocytes were stimulated with these serotypes, different patterns of t-helper (th)1 and th17-type of immune responses were reported. recently, two new th phenotypes have been described and named th9 and th22 lymphocytes; however, their role in the pathogenesis of periodontitis remains unclear. this study aimed to investigate the potential th9 and/or th22 lym ... | 2017 | 28189882 |
| development of a flow chamber system for the reproducible in vitro analysis of biofilm formation on implant materials. | since the introduction of modern dental implants in the 1980s, the number of inserted implants has steadily increased. implant systems have become more sophisticated and have enormously enhanced patients' quality of life. although there has been tremendous development in implant materials and clinical methods, bacterial infections are still one of the major causes of implant failure. these infections involve the formation of sessile microbial communities, called biofilms. biofilms possess unique ... | 2017 | 28187188 |
| presence of aggregatibacter actinomycetemcomitans in saliva and cardiac tissue samples of children with congenital heart disease. | the purpose of this study was to analyze the presence of aggregatibacter actinomycetemcomitans in saliva and cardiac tissue samples of children requiring cardiac surgery in istanbul, turkey. | 2017 | 28169262 |
| application of diverse natural polymers in the design of oral gels for the treatment of periodontal diseases. | the objective of this study was to prepare periodontal gels using natural polymers such as badam gum, karaya gum and chitosan. these gels were tested for their physical and biochemical properties and assessed for their antibacterial activity against aggregatibacter actinomycetemcomitans and streptococcus mutans, two pathogens associated with periodontal disease. badam gum, karaya gum and chitosan were used to prepare gels of varying concentrations. moxifloxacin hydrochloride, a known antimicrobi ... | 2017 | 28144851 |
| incorporation of silver nanoparticles on the surface of orthodontic microimplants to achieve antimicrobial properties. | microbial aggregation around dental implants can lead to loss/loosening of the implants. this study was aimed at surface treating titanium microimplants with silver nanoparticles (agnps) to achieve antibacterial properties. | 2017 | 28127534 |
| effect of opposing implant prostheses on periodontal pathogens in dentures: a pilot study. | an understanding of the presence of periodontal pathogens in denture plaque is important for the treatment of patients with edentulism. however, current data are limited and inconclusive. | 2017 | 28126355 |
| effects of periodontal treatment on primary sjȫgren's syndrome symptoms. | the aim of this longitudinal prospective study was to evaluate the effects of periodontal treatment on the clinical, microbiological and immunological periodontal parameters, and on the systemic activity (essdai) and subjective (esspri) indexes in patients with primary sjögren's syndrome (pss). twenty-eight female patients were divided into four groups: pss patients with or without chronic periodontitis (scp, sc, respectively), and systemically healthy patients with or without chronic periodonti ... | 2017 | 28099577 |
| enzymatic kinetics of the quinol peroxidase of an aggressive periodontopathic bacterium. | aggregatibacter actinomycetemcomitans is an oral pathogen for aggressive periodontitis, and encodes a triheme c-containing membrane-bound enzyme, quinol peroxidase (qpo) that catalyzes peroxidase activity using quinol in the respiratory chain. in the present work, we have characterized recombinant qpo purified from the membrane fraction of escherichia coli harboring a plasmid containing qpo gene. irreversible inactivation of qpo by high concentration of h2o2 exhibited pseudo-first order kinetics ... | 2017 | 28082720 |
| bacterial short-chain fatty acid metabolites modulate the inflammatory response against infectious bacteria. | short-chain fatty acids (scfas), predominantly acetic, propionic, and butyric acids, are bacterial metabolites with an important role in the maintenance of homeostasis due to their metabolic and immunomodulatory actions. some evidence suggests that they may also be relevant during infections. therefore, we aimed to investigate the effects of scfas in the effector functions of neutrophils to an opportunistic pathogenic bacterium, aggregatibacter actinomycetemcomitans. using a subcutaneous model t ... | 2017 | 28070968 |
| secretable small rnas via outer membrane vesicles in periodontal pathogens. | micrornas (mirnas) have been shown to be major regulators of eukaryotic gene expression. however, bacterial rnas comparable in size to eukaryotic mirnas (18-22 nucleotides) have received little attention. recently, a novel class of small rnas similar in size to mirnas (mirna-size, small rnas or msrnas) have also been found in several bacteria. like mirnas, msrnas are approximately 15 to 25 nucleotides in length, and their precursors are predicted to form a hairpin loop secondary structure. here, ... | 2017 | 28068479 |
| the evaluation of cultivable microbiota profile in patients with secondary endodontic infection before and after photo-activated disinfection. | secondary/persistent endodontic infection can be the outcome of failure of endodontic treatment. photo-activated disinfection (pad) can be a useful adjunct to mechanical and antimicrobial agents in eliminating endopathogenic microorganisms. in this study, we evaluated the effect of pad on diversity and count of microbiota related to secondary/persistent endodontic infections. | 2017 | 28249746 |
| the use of bacteriophages to biocontrol oral biofilms. | infections induced by oral biofilms include caries, as well as periodontal, and peri-implant disease, and may influence quality of life, systemic health, and expenditure. as bacterial biofilms are highly resistant and resilient to conventional antibacterial therapy, it has been difficult to combat these infections. an innovative alternative to the biocontrol of oral biofilms could be to use bacteriophages or phages, the viruses of bacteria, which are specific, non-toxic, self-proliferating, and ... | 2017 | 28108235 |
| influence of aae autotransporter protein on adhesion and biofilm formation by aggregatibacter actinomycetemcomitans. | the periodontopathogen aggregatibacter actinomycetemcomitans colonizes oral cavity by binding to and invading epithelial cells as well as by participating in biofilms formed on hard surfaces. aae, an autotransporter protein, is implicated in bacterial adhesion to epithelial cells. due to the multiple functions of bacterial autotransporter proteins, this study aimed to evaluate the role of aae in a. actinomycetemcomitans ability to adhere to both saliva-coated hydroxyapatite (sha) and biofilm. an ... | 2017 | 27224556 |
| systemic aggregatibacter actinomycetemcomitans leukotoxin-neutralizing antibodies in periodontitis. | leukotoxin (ltx) expressed by aggregatibacter actinomycetemcomitans is a powerful exotoxin, which can cause imbalance in host response. immunoreactivity to ltx is a marker for presence of leukotoxic a. actinomycetemcomitans, a presence that may modify the disease pattern of colonized individuals. the aim of the present study is to examine presence of systemic immunoreactivity to a. actionmycetemcomitans ltx with respect to clinical and inflammatory findings in individuals with or without periodo ... | 2017 | 27523521 |
| dataset of the proteome of purified outer membrane vesicles from the human pathogen aggregatibacter actinomycetemcomintans. | the gram-negative bacterium aggregatibacter actinomycetemcomitans is an oral and systemic pathogen, which is linked to aggressive forms of periodontitis and can be associated with endocarditis. the outer membrane vesicles (omvs) of this species contain effector proteins such as cytolethal distending toxin (cdt) and leukotoxin (ltxa), which they can deliver into human host cells. the omvs can also activate innate immunity through nod1- and nod2-active pathogen-associated molecular patterns. this ... | 2017 | 28050585 |
| quantitative analysis of periodontal pathogens using real-time polymerase chain reaction (pcr). | the quantitative polymerase chain reaction (qpcr) is a variant of pcr aimed to detect and quantify a targeted dna molecule through the addition of probes labeled with fluorescent molecules that emit fluorescence within each amplification cycle, what results in fluorescence values proportional to the amount of accumulated pcr product. this chapter presents the detailed procedures for quantification of different periodontal pathogens (porphyromonas gingivalis, aggregatibacter actinomycetemcomitans ... | 2017 | 27924595 |
| survival of an aggregatibacter actinomycetemcomitans quorum sensing luxs mutant in the mouths of rhesus monkeys: insights into ecological adaptation. | experiments were designed to explore a prominent autoinducer-2 (ai-2) producing gene (luxs) related to colonization and survival of aggregatibacter actinomycetemcomitans, a low abundance member of the indigenous flora, that forms a key component of the dysbiotic flora in localized aggressive periodontitis. the luxs gene was disrupted in a primate strain of a. actinomycetemcomitans prior to implantation into the oral cavity of rhesus monkeys (rh). the colonization efficiency of the luxs mutant (r ... | 2017 | 28383798 |
| evaluation of the antibacterial potential of liquid and vapor phase phenolic essential oil compounds against oral microorganisms. | the aim of the present study was to determine the antibacterial activities of the phenolic essential oil (eo) compounds hinokitiol, carvacrol, thymol, and menthol against oral pathogens. aggregatibacter actinomycetemcomitans, streptococcus mutans, methicillin-resistant staphylococcus aureus (mrsa), and escherichia. coli were used in this study. the minimum inhibitory concentrations (mics), minimum bactericidal concentrations (mbcs), bacterial growth curves, temperature and ph stabilities, and sy ... | 2017 | 27681039 |
| rosuvastatin inhibits interleukin (il)-8 and il-6 production in human coronary artery endothelial cells stimulated with aggregatibacter actinomycetemcomitans serotype b. | rosuvastatin exhibits anti-inflammatory effects and reduces periodontal diseases and atherosclerosis; however, its role in regulating periodontopathogen-induced endothelial proinflammatory responses remains unclear. the purpose of this study is to determine whether rosuvastatin can reduce the proinflammatory response induced by aggregatibacter actinomycetemcomitans (aa) in human coronary artery endothelial cells (hcaecs). | 2017 | 27739345 |
| a double-blind randomized trial comparing implants with laser-microtextured and machined collar surfaces: microbiologic and clinical results. | the purpose of this study was to compare the clinical outcome of and determine the differences in periodonto-pathogenic microbiota around two types of implant collar surfaces: laser-microtextured (test) vs machined (control). | 2017 | 27632268 |
| microbial characteristics of peri-implantitis: a case-control study. | the aim of this case-control study is to compare oral microbiologic characteristics of patients with healthy peri-implant conditions and patients with peri-implantitis and to explore the influence of various patient- and implant-related factors on microbiologic characteristics. | 2017 | 27666672 |
| mitogen-activated protein kinase 2 signaling shapes macrophage plasticity in aggregatibacter actinomycetemcomitans-induced bone loss. | aggregatibacter actinomycetemcomitans is associated with aggressive periodontal disease, which is characterized by inflammation-driven alveolar bone loss. a. actinomycetemcomitans activates the p38 mitogen-activated protein kinase (mapk) and mapk-activated protein kinase 2 (mk2) stress pathways in macrophages that are involved in host responses. during the inflammatory process in periodontal disease, chemokines are upregulated to promote recruitment of inflammatory cells. the objective of this s ... | 2017 | 27795356 |
| regulation of matrix metalloproteinase secretion by green tea catechins in a three-dimensional co-culture model of macrophages and gingival fibroblasts. | elevated levels of matrix metalloproteinases (mmps) have been associated with the active phases of tissue and bone destruction in periodontitis, an inflammatory disease characterized by a significant breakdown of tooth support. in the present study, we used a three-dimensional (3d) co-culture model of macrophages and gingival fibroblasts to investigate the ability of a green tea extract and its major constituent epigallocatechin-3-gallate (egcg) to regulate the secretion of mmp-3, -8, and -9. | 2017 | 27825679 |
| virulence factors associated with aggregatibacter actinomycetemcomitans and their role in promoting periodontal diseases. | 2017 | 27623711 | |
| microbiological and clinical assessment of the abutment and non-abutment teeth of partial removable denture wearers. | the aim of this study was assessing the changes in both clinical and microbiological parameters of healthy individuals after rehabilitation with removable partial denture (rpd). | 2017 | 27825678 |
| functional contributions of positive charges in the pore-lining helix 3 of the bordetella pertussis cyaa-hemolysin to hemolytic activity and ion-channel opening. | the bordetella pertussis cyaa-hemolysin (cyaa-hly) domain was previously demonstrated to be an important determinant for hemolysis against target erythrocytes and ion-channel formation in planar lipid bilayers (plbs). here, net-charge variations in the pore-lining helix of thirteen related rtx cytolysins including cyaa-hly were revealed by amino acid sequence alignments, reflecting their different degrees of hemolytic activity. to analyze possible functional effects of net-charge alterations on ... | 2017 | 28300777 |
| diabetic lactoferrin deficient mice demonstrates greater susceptibility to experimental periodontal disease. | the objective of this study is to detrmine whether alloxan-induced diabetic lactoferrin knockout (lfko(-/-)) mice are more susceptible to periodontal disease caused by aggregatibacter actinomycetemcomitans compared to the diabetic wild-type (wt) mice. diabetes was induced in mice by a single dose of alloxan (60 mg/kg) injected intravenously. mice were categorized as diabetic when blood glucose levels >250 mg/dl were measured on the 7(th) day after the injection. periodontal disease was experimen ... | 2017 | 26855973 |
| influence of a triclosan toothpaste on periodontopathic bacteria and periodontitis progression in cardiovascular patients: a randomized controlled trial. | triclosan/copolymer toothpaste is effective in controlling plaque and gingivitis and in slowing the progression of periodontitis. this study describes its influence on microbiological and clinical outcomes, over a 5-year period, in patients with established cardiovascular disease (cvd). | 2017 | 26932733 |
| a bacterial-biofilm-induced oral osteolytic infection can be successfully treated by immuno-targeting an extracellular nucleoid-associated protein. | periodontal disease exemplifies a chronic and recurrent infection with a necessary biofilm component. mucosal inflammation is a hallmark response of the host seen in chronic diseases, such as colitis, gingivitis, and periodontitis (and the related disorder peri-implantitis). we have taken advantage of our recently developed rat model of human peri-implantitis that recapitulates osteolysis, the requirement of biofilm formation, and the perpetuation of the bona fide disease state, to test a new th ... | 2017 | 26931773 |
| antimicrobial efficacy of tulsi leaf (ocimum sanctum) extract on periodontal pathogens: an in vitro study. | periodontitis is an infection of the periodontal complex with severe forms of disease associated with specific bacteria colonizing the subgingival area. widespread use of drugs has resulted in the emergence of side effects, uncommon infections, and resistance. plant medicine like tulsi has been used in many clinical conditions, and it appears to be a suitable alternative to manage conditions affecting the oral cavity. hence, the objective was to assess the in vitro antimicrobial activity of tuls ... | 2017 | 27143825 |
| occurrence of aggregatibacter actinomycetemcomitans in indian chronic periodontitis patients and periodontally healthy adults. | aggregatibacter actinomycetemcomitans (aa), an important primary periodontal pathogen, is known for its strong virulence characteristics that cause periodontal disease. we investigated aa occurrence in indian individuals using culture and 16 s rdna polymerase chain reaction (pcr). | 2017 | 27143824 |
| bactericidal effect of erbium-doped yttrium aluminum garnet laser and photodynamic therapy on aggregatibacter actinomycetemcomitans biofilm on implant surface. | peri-implantitis is a common complication of dental implants. the first step of treatment is elimination of bacterial biofilm and disinfection of the implant surface. this study sought to compare the effects of an erbium-doped yttrium aluminum garnet (er:yag) laser, photodynamic therapy using an indocyanin green-based photosensitizer (icg-based ps) and diode laser, toluidine blue o (tbo) photosensitizer and light-emitting diode (led) light source, and 2% chlorhexidine (chx) on biofilm of aggrega ... | 2017 | 27183085 |
| a novel intrinsically disordered outer membrane lipoprotein of aggregatibacter actinomycetemcomitans binds various cytokines and plays a role in biofilm response to interleukin-1β and interleukin-8. | intrinsically disordered proteins (idps) do not have a well-defined and stable 3-dimensional fold. some idps can function as either transient or permanent binders of other proteins and may interact with an array of ligands by adopting different conformations. a novel outer membrane lipoprotein, bacterial interleukin receptor i (bilri) of the opportunistic oral pathogen aggregatibacter actinomycetemcomitans binds a key gatekeeper proinflammatory cytokine interleukin (il)-1β. because the amino aci ... | 2017 | 27459270 |
| evaluation of antimicrobial action of carie care™ and papacarie duo™ on aggregatibacter actinomycetemcomitans a major periodontal pathogen using polymerase chain reaction. | in the present scenario, we are made available with chemomechanical caries removal system containing a natural proteolytic enzyme for the ease in the excavation of infected dentine. the additive action for these agents is providing antimicrobial and anti-inflammatory properties. | 2017 | 26681861 |