Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| disseminated extraintestinal isosporiasis in a patient with acquired immune deficiency syndrome. | this report describes a 38-year-old male homosexual with acquired immune deficiency syndrome (aids) and a history of isospora belli infestation treated with trimethoprim-sulfamethoxazole. at autopsy, intracellular and extracellular i. belli organisms were identified in the mucosa and lamina propria of small and large intestine as well as in mesenteric and tracheobronchial lymph nodes, where an associated granulomatous reaction was also present. until now, isosporiasis has been observed as intrac ... | 1987 | 3826017 |
| shigella bacteremia in patients with the acquired immune deficiency syndrome. | bacteremic infection with shigella species is extremely rare in adults. we report five cases of shigella bacteremia in adult men that occurred at our hospital over a 2-yr period. four of these cases occurred in patients with the acquired immune deficiency syndrome. the fifth patient had evidence of cell-mediated immunodeficiency, but did not meet centers for disease control criteria for the acquired immune deficiency syndrome. shigellemia is a potentially fatal but treatable complication of the ... | 1987 | 3565339 |
| cytotoxicity of shiga toxin for primary cultures of human colonic and ileal epithelial cells. | shiga toxin purified from shigella dysenteriae 1 was cytotoxic to cultured epithelial cells from human colon and ileum. the cytotoxicity, which affected only about 50% of treated cells, was neutralized by rabbit antiserum monospecific for shiga toxin and mediated by protein synthesis inhibition. | 1987 | 3570477 |
| campylobacter hyointestinalis associated with human gastrointestinal disease in the united states. | campylobacter hyointestinalis was isolated from stool specimens of four persons, all of whom were experiencing nonbloody, watery diarrhea. the youngest (8 months of age) and the oldest (79 years of age) individuals were females, and the other two were homosexual men. c. hyointestinalis was the only clinically significant pathogen isolated from stool specimens for three of the individuals. in case 3 (involving a 37-year-old homosexual man), entamoeba histolytica and shigella sonnei were also pres ... | 1987 | 3571477 |
| production of a unique cytotoxin by campylobacter jejuni. | campylobacter jejuni is an important diarrheal pathogen worldwide; the mechanisms by which it causes disease remain unclear. because of its association with inflammatory diarrhea, we postulated that c. jejuni might produce a cytotoxin similar to that produced by shigella sp., enterohemorrhagic escherichia coli o157, or clostridium difficile. filtrates of 12 polymyxin-treated isolates of c. jejuni were placed on hela cells (sensitive to shiga toxin cytotoxicity) and chinese hamster ovary (cho) ce ... | 1987 | 3653987 |
| infections in british clinical laboratories, 1984-5. | during 1984-5 this continuing survey showed that 41 infections occurred in the staff of 193 laboratories, representing 23,043.5 person years of exposure. the community was the probable source of two cases each of hepatitis a and b, one of tuberculosis, two of campylobacter enteritis, and 12 of norwalk viral diarrhoea. occupational exposure was the probable cause of six hepatitis b infections (affecting haematology, biochemistry, and microbiology staff), three of tuberculosis (affecting mortuary ... | 1987 | 3654983 |
| [home-coming diarrhea. presentation of a clinical case]. | the homing diarrhea, described by duhamel and rey in 1980, represents a gastrointestinal disease which affects children of emigrants coming back from a trip to their country of origin and may become so serious to cause death secondary to dehydration or septicemia. the poor nutritional conditions at the beginning of the trip (secondary to low socioeconomic situation) and the onset of acute enteritis during the travel, caused by salmonella or shigella, e. coli, campylobacter, myceti, giardia lambl ... | 1987 | 3658808 |
| the mode of action of shiga toxin on peptide elongation of eukaryotic protein synthesis. | the effect of shiga toxin, from shigella dysenteriae 1, on the component reactions of peptide elongation were investigated. enzymic binding of [3h]phenylalanine-trna to reticulocyte ribosomes was inhibited by 50% at 7 nm toxin. elongation factor 1 (eef-1)-dependent gtpase activity was also inhibited. both reactions were not restored by addition of excess eef-1 protein. in contrast, toxin concentrations of 200 nm were required to inhibit by 50% the elongation factor 2 (eef-2)-dependent translocat ... | 1987 | 3663122 |
| leukemoid reaction in shigella dysenteriae type 1 infection. | 1987 | 3666850 | |
| crystal violet ring response as a marker for in vitro detection of pathogenic vibrio parahaemolyticus & shigella spp. | 1987 | 3666851 | |
| properties of equine anti-lipopolysaccharide hyperimmune plasma: binding to lipopolysaccharide and bactericidal activity against gram-negative bacteria. | anti-lipopolysaccharide equine hyperimmune plasma (anti-lps), which has been used successfully to treat lps (endotoxin)-mediated disorders, has been further characterised. igg present in anti-lps had the highest affinity for lps prepared from salmonella typhimurium, s. typhi, s. abortus equi and shigella flexneri and intermediate affinity for escherichia coli o55:b5, e. coli o127:b8 and s. enteritidis. anti-lps destroyed by means of complement activation a wide range of gram-negative bacteria, i ... | 1987 | 3669052 |
| [evaluation of the effectiveness of bacteriologic studies in detecting salmonella and shigella in healthy persons under sanitary surveillance in poland 1976-1985]. | 1987 | 3669801 | |
| serotyping of plesiomonas shigelloides strains with our own antigenic scheme. an attempted epidemiological study. | thirty-four o and 19 h sera were used to test 169 strains of plesiomonas shigelloides from several countries of three continents. the most frequent o serovar was o17 (o antigen identical with shigella sonnei phase i) which occurred in the oh serovar combinations: o17:c, o17:d and o17:f. some oh serovars were represented by a single strain; others, despite the small number of strains in hand, were evidently ubiquitous, having been isolated in mutually distant countries or at long time intervals. ... | 1987 | 3673335 |
| production of bacteriocin-like agents of budvicia aquatica and "pragia fontium". | in cultures of 9 budvicia aquatica and 5 "pragia fontium" strains production of distinct bacteriocin-like agents was proved. it is suggested to call them aquaticins and fonticins. they display strain specific antibacterial activities towards both genera; however, they are not active towards escherichia coli or shigella sonnei indicators. their inhibition zones are conspicuously narrow (turbid or clear). their spontaneous production may be enhanced by uv-induction of producing bacteria. they are ... | 1987 | 3673338 |
| empirical treatment of shigella dysentery with trimethoprim: five-day course vs. single dose. | fifty-three adults hospitalized with shigella dysentery were empirically treated with trimethoprim (200 mg) twice/day for 5 days, a single dose of trimethoprim (600 mg), or placebo in a randomized double-blind trial. during the first 24 hr of therapy, there was a reduction in the number of stools in 18/21 (86%) of patients treated with the 5-day regimen (trimethoprim-5) and 13/15 (87%) of patients treated with a single dose (trimethoprim-1), compared with 7/17 (41%) of the placebo group (p less ... | 1987 | 3688315 |
| a prospective epidemiologic study of shigellosis in the israel defense forces: implications for the use of shigella vaccines. | recent development of new shigella vaccines has renewed interest in the current epidemiology of shigellosis in endemic regions. a prospective epidemiologic study of 5,774 soldiers was carried out in the israel defense forces, between the months of may and september 1984. shigellosis was found to be responsible for half the diarrhea epidemics and only rarely presented as sporadic cases of diarrhea. the epidemics occurred after exposure to field conditions, whereas under fixed military base condit ... | 1987 | 3692749 |
| nalidixic acid in resistant shigella infection. | 1987 | 3693298 | |
| chemical characterization of enterobacterial common antigen isolated from plesiomonas shigelloides atcc 14029. | serologically characterized samples of enterobacterial common antigen (eca) from plesiomonas shigelloides, salmonella montevideo and shigella sonnei were investigated by chemical methods including methylation and nmr techniques. all showed the same sugar composition and contained a lipid moiety with palmitic acid as main fatty acid and with a phosphodiester group. additional enzymatic studies, reported in the preceding paper, provided evidence that the lipid moiety is an l-glycerophosphatidyl re ... | 1987 | 3816787 |
| qualitative and quantitative determination of enterobacterial common antigen (eca) with monoclonal antibodies: expression of eca by two actinobacillus species. | the presence and quantity of the enterobacterial common antigen (eca) in several species belonging to the family enterobacteriaceae as well as to other gram-negative families were determined by a solid-phase enzyme-linked immunosorbent assay system and western blotting by using mouse monoclonal antibodies specific for eca. except for erwinia chrysanthemi, previously known to be an exception, all species known or presumed to belong to enterobacteriaceae produced eca (89 of 90 species). most speci ... | 1987 | 3818929 |
| rapid catalase supplemental test for identification of members of the family enterobacteriaceae. | a simple, rapid, semiquantitative slide catalase test useful for differentiating members of the family enterobacteriaceae is described. judging by the time required for appearance of oxygen bubbles in 3% hydrogen peroxide, the immediate catalase reactors were yersinia, serratia, proteus, morganella, providencia, cedecea, and hafnia spp. the delayed catalase reactors were escherichia, shigella, klebsiella, enterobacter, salmonella, citrobacter, edwardsiella, kluyvera, and tatumella spp. this info ... | 1987 | 3818934 |
| incidence of shigellosis and multi-drug resistant shigellae: a 10 year study. | between 1976 and 1985 a total of 12,709 faecal samples were cultured. from these samples 760 strains (6.0%) of shigellae were isolated. shigella flexneri was the commonest isolate (55%), followed by s. dysenteriae (29.7%). a total of 15.7% of children under 5 years of age were positive for shigellae. the rate of isolation was lower during the winter months than in the summer months. only 25 strains (3.3%) were sensitive to all five antibiotics tested. a total of 617 (81.2%) were resistant to thr ... | 1987 | 3820357 |
| multiyear study of sludge application to farmland: prevalence of bacterial enteric pathogens and antibody status of farm families. | we describe our experience with the isolation of salmonellae from sewage sludge from four treatment plants in different geographic areas of ohio. over 3 years, we isolated salmonellae 50 times from 311 sludge samples. most isolations were made after enrichment in selenite broth (bbl microbiology systems, cockeysville, md.). the largest proportion of isolations came from the plant serving the population of columbus, a large metropolitan area. a significantly greater number of isolations from this ... | 1987 | 3606093 |
| [shigella and salmonella isolated in burundi from 1980 to 1985]. | data are presented on 633 strains of shigella, and 197 strains of salmonella, isolated in bujumbura, capital of burundi, between 1980 and 1985. the epidemiology of shigellosis is dominated by the resurgence of the shiga bacillus and by its increasing resistance against all available antibacterial drugs. salmonellosis is caused by a limited range of serotypes, most of them, with the exception of the typhoid bacillus, becoming multiresistant. bacteriaemia with s. enteritidis is a frequent complica ... | 1987 | 3608004 |
| bacterial survey of well water--tehran, iran. | one hundred twenty-one wells throughout the city of tehran were monitored during the four seasons of the year for the presence of bacterial indicators of fecal pollution. none of the sites monitored was connected to tehran's main water distribution system but represented drinking water sources for small communities, individual factories, hospitals, etc. only 17 samples showed no indication of fecal bacterial contamination throughout the study. the remaining sites were positive for at least one o ... | 1987 | 3608933 |
| epidemiology and etiology of diarrhea in colony-born macaca nemestrina. | the epidemiology of diarrhea in colony-born m. nemestrina was studied in 205 neonates and infants in an infant primate research laboratory (iprl), and in 248 neonates, juveniles and adolescents up to 4 years of age at a separate breeding and holding facility (primate field station, pfs). computerized medical records of individual animals over a 5-year period were analyzed to determine the incidence of diarrhea; age, duration and number of episodes; mortality and etiology. the incidence of diarrh ... | 1987 | 3613510 |
| antimicrobial activity of benzylisoquinoline alkaloids. | the antimicrobial in vitro activity of 14 benzylisoquinoline alkaloids was investigated by agar diffusion and agar dilution methods against several genera of microorganisms that included streptococcus, staphylococcus, bacillus, lysteria, escherichia, salmonella, klebsiella, pseudomonas, enterobacter, serratia, shigella, mycobacterium and candida. anolobine was the most active compound against grampositive bacteria with mic90 between 12 and 50 mg/l; less active were anonaine, lysicamine and lirio ... | 1987 | 3615557 |
| two year study of cryptosporidium infection. | during a two year period 48 children admitted to hospitals in the blackburn district were found to have cryptosporidium in stool samples. cryptosporidium accounted for 6% of the 742 cases of childhood gastroenteritis, being as common as campylobacter. altogether, 89% of children with cryptosporidiosis had diarrhoea, which was usually offensive and watery, and 80% vomited, the mean duration of both symptoms being six days. a substantial proportion were moderately ill with dehydration and persiste ... | 1987 | 3619472 |
| shigella septicemia: a reminder. | 1987 | 3625731 | |
| predictive value of stool examination in acute diarrhea. | we prospectively evaluate the value of fecal blood and fecal leukocytes in predicting whether acute diarrhea in adults is associated with a stool culture positive for a bacterial pathogen. one hundred thirteen patients, aged 19 to 50 years, seen in a two-year period in an urban adult outpatient setting underwent stool culture for the presenting symptom of diarrhea. heterosexual men represented 48% of the cohort, women represented 17%, and homosexual men represented 35%. overall, 53 (47%) of the ... | 1987 | 3632285 |
| erythromycin-resistant campylobacter infections in thailand. | erythromycin therapy was compared with no treatment in a prospective trial of acute diarrheal disease among 100 infants in an orphanage in bangkok. within 24 h of the onset of diarrhea, 50 children received erythromycin ethylsuccinate (40 mg/kg per day) in four divided doses for 5 days. campylobacter jejuni isolated from 31, campylobacter coli isolated from 21, and shigella spp. isolated from 21 of 100 children were the most commonly recognized pathogens; use of a sensitive, nonselective method ... | 1987 | 3579261 |
| clinical and biochemical significance of toxin production by aeromonas hydrophila. | production of cytotoxin and enterotoxin by aeromonas strains obtained from stools of 50 children in mexico and texas and from blood of 9 children with sepsis was determined. results were correlated with clinical features of infected children as well as with biochemical traits of aeromonas strains. cytotoxin was produced by 40 of 42 aeromonas strains (95%) isolated from stools of children with diarrhea, by all 8 isolates from stools of well children, and by all 9 isolates from children with sepsi ... | 1987 | 3584426 |
| human carriage of yersinia spp. | examination of faecal specimens for the presence of yersinia spp. during a 1-year period yielded isolations from 3.5% of patients. salmonella spp., campylobacter spp. and shigella spp. were isolated from 2.5%, 2.8% and 0.34% of patients respectively. most isolates belonged to y. enterocolitica biotype 1 (53%) and y. frederikseni (39%). the most frequently encountered serotypable strains were serotypes o 5.27 and o 6.30. serotype o 3, the commonly recognised pathogenic strain, was not isolated in ... | 1987 | 3585967 |
| effect of water coagulation by seeds of moringa oleifera on bacterial concentrations. | the effects of a sudanese water purification method traditionally used in sudan to treat turbid waters were studied with respect to turbidity reduction and removal of faecal indicator bacteria as well as selected enteric bacterial pathogens. water treatment was performed at 30 degrees c with moringa oleifera seed material as a coagulant, and the technique employed corresponded closely to that used to clarify turbid water in sudanese villages. a turbidity reduction of 80.0-99.5% paralleled by a p ... | 1987 | 3586089 |
| pediatric gastroenteritis in primary care and in hospitalized patients. | one-hundred and fifty-seven consecutive children below seven years of age (primary care n = 48, hospitalized patients n = 109) with acute gastroenteritis of assumed infectious origin were studied. rotavirus was demonstrated by electron microscopy of faeces in 44% of all patients. the occurrence of rotavirus among patients in primary care, 15%, was significantly lower than among hospitalized patients, 57% (p less than 0.01). adenovirus was isolated in six per cent and enterovirus in two per cent ... | 1987 | 3589234 |
| clinical impact of stool cultures for campylobacter in adults with acute or chronic diarrhea. | campylobacter jejuni has emerged as a frequent cause of diarrhea. during a 12-month period at the houston veterans administration medical center, we isolated c jejuni from 3.4% of the 290 stool cultures from patients with diarrhea. this compared to an isolation rate of 4.1% for salmonella and 3.1% for shigella. during the same period, 17 additional cases of campylobacter-associated diarrhea were identified in adults at the two other baylor college of medicine teaching hospitals. we correlated th ... | 1987 | 3589763 |
| comparative in vitro activity of the new oral cephalosporin bmy-28100. | using a broth microdilution method, the in vitro activity of bmy-28100 against 365 clinical strains of commonly isolated bacteria was determined. bmy-28100 showed good activity against streptococci, methicillin-susceptible staphylococci, salmonella spp., shigella spp., and beta-lactamase producing branhamella catarrhalis and haemophilus influenzae. against susceptible strains of these organisms, bmy-28100 showed activity comparable to that of penicillin g, ampicillin, co-trimoxazole, erythromyci ... | 1987 | 3595580 |
| current problems in antimicrobial therapy for bacterial enteric infection. | trimethoprim/sulfamethoxazole is currently considered the treatment of choice for shigellosis and severe travelers' diarrhea. the problem with this combination regimen is inactivity against campylobacter jejuni strains and other bacterial enteropathogens showing in vitro resistance to the drug. resistance to trimethoprim/sulfamethoxazole among enteric pathogens has occurred frequently in certain areas of the world. a study of the in vitro susceptibility of enteric bacterial pathogens isolated fr ... | 1987 | 3555056 |
| clinical efficacy of ciprofloxacin compared with placebo in bacterial diarrhea. | in a double-blind, randomized trial, 85 adult patients with acute diarrhea (more than three watery stools per day) received either 500 mg of ciprofloxacin twice daily or placebo for five days. seventy-six patients were evaluated, 38 patients in the ciprofloxacin group (16 with salmonella species, 19 with campylobacter jejuni, and three with shigella species) and 38 patients in the placebo group (21 with salmonella species, 11 with c. jejuni, and six with shigella species). the duration of fever ... | 1987 | 3555057 |
| neonatal shigellosis: a case report. | 1987 | 3556231 | |
| shigellosis in the south-western cape of good hope 1968-85. | during the period 1968-85 shigella organisms were isolated from stool specimens of 1562 patients attending tygerberg hospital, situated in the south-western province of the cape of good hope of the republic of south africa. shigella flexneri (72% of patients) was the commonest subgroup identified. sh. sonnei was the second-commonest isolate (20%), with smaller numbers of sh. boydii (5%) and sh. dysenteriae (3%). sh. dysenteriae has not been isolated since 1979. in 1985 30% of isolates were resis ... | 1987 | 3556443 |
| in vitro and in vivo pathogenicity of plesiomonas shigelloides. | epidemiologic evidence suggests that plesiomonas shigelloides is an enteric pathogen. we conducted in vitro, animal, and volunteer studies on p. shigelloides isolates from patients with diarrhea. five strains gave a negative keratoconjunctivitis reaction in guinea pigs and did not invade hela cells. genetic probes for heat-stable enterotoxins related to those of enterotoxigenic escherichia coli and for gene sequences common to the invasiveness plasmids of shigella spp. and enteroinvasive e. coli ... | 1987 | 3557621 |
| shigella keratitis: a report of two cases and a review of the literature. | 1987 | 3547292 | |
| multiresistant strains of enteric pathogens cultured from stools of acute cases of diarrhea in cairo, egypt. | 1987 | 3509426 | |
| local and systemic antibody responses to shigella infection in rhesus monkeys. | 1987 | 3549918 | |
| [isolation and characteristics of shigella antigens during the course of dysentery]. | in 2,436 fecal samples and 1,272 urinary samples taken from 633 patients with dysentery caused by s. flexneri, s. newcastle and s. sonnei, shigella antigens were detected by means of the passive hemagglutination test with antibody diagnostic agents and the antibody neutralization test. the antigen-binding activity of shigellae in urine dynamically increased in the course of dysentery. the comparison of the parallel results of both serological tests made it possible to evaluate the dispersion of ... | 1987 | 3551415 |
| antibacterial activity and mechanism of action of 3'-azido-3'-deoxythymidine (bw a509u). | the thymidine analog 3'-azido-3'-deoxythymidine (bw a509u; azidothymidine [azt]) had potent bactericidal activity against many members of the family enterobacteriaceae, including strains of escherichia coli, salmonella typhimurium, klebsiella pneumoniae, shigella flexneri, and enterobacter aerogenes. azt also had bactericidal activity against vibrio cholerae and the fish pathogen vibrio anguillarum. azt had no activity against pseudomonas aeruginosa, gram-positive bacteria, anaerobic bacteria, m ... | 1987 | 3551832 |
| congo red binding phenotype is associated with hemin binding and increased infectivity of shigella flexneri in the hela cell model. | wild-type isolates of shigella flexneri bind the dye congo red from solid media, thus producing red (crb+) colonies. mutants which fail to bind the dye produce white colonies (crb-) and are avirulent in a variety of systems. in s. flexneri the ability to bind congo red correlates with the ability to bind hemin and protoporphyrin ix. binding of hemin by crb+ s. flexneri was observed both in solid media and in liquid assays. results of competition experiments suggest that congo red and hemin bind ... | 1987 | 3553000 |
| review of the 4-quinolones. | improvements in antimicrobial activity and pharmacokinetics have moved the 4-quinolones into the forefront of antibiotic research. the 4-quinolones are analogues of nalidixic acid, and there are presently at least six agents in this group under investigation in the united states. it is difficult to generalize their clinical usefulness, since these agents exhibit different pharmacokinetic profiles, antimicrobial activity (with varied minimum inhibitory concentrations among similar organisms), and ... | 1987 | 3553053 |
| rapid automated method for screening of enteric pathogens from stool specimens. | a total of 800 colonies suggestive of salmonella, shigella, or yersinia species isolated on stool differential agar media were inoculated onto both conventional biochemical test media (triple sugar iron agar, urea agar, and phenylalanine agar) and entero pathogen screen cards of the automicrobic system (vitek systems, inc., hazelwood, mo.). based on the conventional tests, the automicrobic system method yielded the following results: 587 true-negatives, 185 true-positives, 2 false-negatives, and ... | 1987 | 3553230 |
| [infectious diarrhea in children. results of a prospective study concerning various epidemiological and clinical aspects of infectious diarrhea in children]. | over a 3-year period (from october 1981 to september 1984) 352 pediatric patients were treated for infectious diarrhea at the division for pediatric infectious diseases, department of pediatrics, university of berne, switzerland. routine diagnostic tests on stool samples included cultures for salmonella, shigella, campylobacter and yersinia, and enzyme-linked immunosorbent assay (rotazyme) for rotaviruses. in selected cases special tests for parasitic pathogens were also performed. the patients ... | 1987 | 3554501 |
| comparison of enterobacterial common antigen from different species by serological techniques. | enterobacterial common antigen (eca) was isolated from a number of selected species (including salmonella montevideo, shigella sonnei and plesiomonas shigelloides) using the extraction method described by männel and mayer [eur. j. biochem. 86, 361-370 (1978)]. eca of all these species behaved identically in enzyme-linked immunosorption assay (elisa) and in its inhibition using monoclonal anti-eca antibodies. immunoblotting showed a ladder-like pattern of at least 20 bands for all preparations te ... | 1987 | 3545822 |
| experimental production of colicin type v of shigella sonnei unusual in nature. | col v factor of fredericq's strain escherichia coli 1.7a was mobilized for a transfer by the r (sm, cm, tc, su) factor of shigella sonnei strain in three component test. the strain e. coli k13 hfrr (a universal indicator for evidence of colicinogeny) and s. sonnei strain became the recipient of both plasmids. s. sonnei, a producer of colicin v has not been found in natural conditions as yet. | 1987 | 3546028 |
| plasmid-mediated early killing of eucaryotic cells by shigella flexneri as studied by infection of j774 macrophages. | in shigella flexneri a 220-kilobase plasmid encodes the ability to invade nonprofessional phagocytes by a mechanism similar to phagocytosis. in this report, the continuous macrophage cell line j774 was used to study the intracellular fate of both invasive and noninvasive strains. pwr100, the virulence plasmid of s. flexneri serotype 5, mediated very efficient and rapid killing of j774 macrophages, as measured by cellular detachment and uptake of trypan blue. for this to occur, the bacteria had t ... | 1987 | 3546130 |
| limitations of using the plasmid pattern as an epidemiological tool for clinical isolates of shigella sonnei. | 1987 | 3543149 | |
| shigellosis in bangladesh. | 1987 | 3543150 | |
| convulsions in childhood shigellosis. clinical and laboratory features in 153 children. | we studied 153 children who experienced convulsions associated with shigellosis. the male-female ratio was 1.2:1.0. thirty-six children had a previous history of febrile convulsions, and 31 children had a family history of convulsive disorder. most of the children were 0.5 to 3 years of age, although 49 (32%) were older than 3 years of age and 20 (13.1%) were older than 5 years of age. all children were febrile; in 75% of the children, the temperature was over 39 degrees c. the majority of the c ... | 1987 | 3544808 |
| ionic requirements for entry of shiga toxin from shigella dysenteriae 1 into cells. | the ionic requirements for entry of shiga toxin into cells were examined by measuring inhibition of protein synthesis after short-term incubations with toxin. the sensitivity of vero cells and hela cells to shiga toxin was strongly dependent on the divalent cation present. vero cells were most sensitive in the presence of cacl2 and srcl2, whereas hela cells were equally sensitive in the presence of mgcl2, srcl2, and cacl2. both cell lines were protected by bacl2, cocl2, and mncl2. inhibitors of ... | 1987 | 3542829 |
| cloning of genes from members of the family enterobacteriaceae with mini-mu bacteriophage containing plasmid replicons. | an in vivo cloning system that uses derivatives of the escherichia coli bacteriophage mu with plasmid replicons has been extended to five different species of the family enterobacteriaceae. mu and these mini-mu replicon elements were introduced into strains of e. coli, shigella flexneri, salmonella typhimurium, citrobacter freundii, and proteus mirabilis by infection, by transformation, or by conjugation with newly constructed broad-host-range plasmids containing insertions of these elements. ly ... | 1987 | 3542967 |
| identification of the carbohydrate receptor for shiga toxin produced by shigella dysenteriae type 1. | the binding of shiga toxin isolated from the bacterium shigella dysenteriae type 1 to a series of glycolipids and to cells or cell homogenates has been studied. bound toxin was detected using either 125i-labeled toxin or specific monoclonal antibody and 125i-labeled anti-antibody. overlay of toxin on thin-layer chromatograms with separated glycolipids and binding to glycolipids coated in microtiter wells established that the toxin specifically bound to gal alpha 1-4gal beta (galabiose) placed te ... | 1987 | 3543013 |
| frequency of escherichia coli o157:h7 isolation from stool specimens. | during a 6-month period, 7252 faeces specimens were examined for escherichia coli serotype o157:h7. forty-nine specimens (0.7%) from 19 patients yielded this organism. escherichia coli o157:h7 was the third most frequently isolated bacterial pathogen, following campylobacter jejuni and (or) salmonella sp. although regional variation between laboratories determined whether campylobacter jejuni or salmonella was the primary bacterial pathogen isolated, e. coli o157:h7 was consistently isolated mor ... | 1987 | 3319115 |
| pathogenesis of shigella infections. | 1987 | 3321018 | |
| inhibition of enteropathogenic escherichia coli (strain rdec-1) adherence to rabbit intestinal brush borders by milk immune secretory immunoglobulin a. | 1987 | 3321942 | |
| enhancement of the secretory iga response to enteropathogens. | 1987 | 3321943 | |
| flanking and internal regions of chromosomal genes mediating aerobactin iron uptake systems in enteroinvasive escherichia coli and shigella flexneri. | we have investigated the presence of the aerobactin system and the location of the aerobactin genes in enteroinvasive strains of escherichia coli. also, we cloned the aerobactin region and its flanking sequences from the chromosome of a strain of shigella flexneri and compared the molecular organization of the aerobactin genes in the two genera. of the 11 enteroinvasive e. coli strains studied, 5 possessed the aerobactin genes, which were located on the chromosome in each case. these strains pro ... | 1987 | 2832521 |
| [activity of restriction endonuclease sso ii in escherichia coli strains transformed by shigella sonnei 47 plasmids]. | the inverse dependence of activity of restriction endonuclease ssoii preparations on the number of low molecular mass plasmids of shigella sonnei transforming escherichia coli recipient cells producing the enzyme has been shown. escherichia coli strain producing efficiently one of two shigella sonnei 47 restriction endonucleases ssoii has been isolated. the producer strain harbours two of the nine shigella sonnei 47 plasmids. one of them p4 codes for ssoii+ phenotype while the other p9 determine ... | 1987 | 2833695 |
| frequency of is1-mediated molecular events in different members of the family enterobacteriaceae. | the numbers of chromosomal copies of the insertion sequence is1 in strains of salmonella typhimurium (0 to 8 copies), shigella sonnei (56 copies), and shigella flexneri (41 copies) isolated in mexico city, mexico, were similar to those reported for these genera isolated in other countries. of the 11 shigella strains studied, all carried several small plasmids; however, in only one of these strains did a small plasmid contain is1, is1 recombination, cointegrate formation mediated by is1 or by the ... | 1987 | 2822657 |
| [molecular and medical aspects of dna modification]. | 1987 | 2823497 | |
| isolation and characterization of is elements repeated in the bacterial chromosome. | shigella sonnei contains repetitive sequences, including an insertion element is1, which can be isolated as double-stranded dna fragments by dna denaturation and renaturation and by treatment with s1 nuclease. in this paper, we describe a method of cloning the is1 fragments prepared by the s1 nuclease digestion technique into phage m13mp8 rfi dna. several clones contained is1, usually with a few additional bases. we isolated and characterized five other repetitive sequences using this method. on ... | 1987 | 2824781 |
| genetic and physical characterization of trimethoprim resistance plasmids from shigella sonnei and shigella flexneri. | analysis of six shigella flexneri and four s. sonnei isolates with trimethoprim (tp) resistance from clinical cases in ontario has shown that, in all isolates, the tp resistance is mediated by gene(s) on conjugative, multiple antibiotic-resistance plasmids. the physical and genetic characterization of these plasmids revealed that there are three different tp resistance plasmids. one group, composed of all six s. flexneri plasmids, consists of plasmids which are about 70 megadaltons (mda) and inh ... | 1987 | 2825949 |
| [mobilization of phase i plasmid in shigella sonnei and stability of its inheritance in rough strains of shigella and escherichia]. | the plasmid pss120, determining the synthesis of species specific i phase antigen of shigella sonnei is mobilized for genetic transfer into e. coli k12 recipient cells with the frequency 12-41%. the frequency depends on the type of mobilized plasmid and recipient strain. the i phase antigen is normally expressed in ii phase recipient cells and in e. coli cells. during mobilization pss120 forms cointegrates representing a recombinant of mobilizing and mobilized plasmids dna. the study of pss120 i ... | 1987 | 2828937 |
| [rotavirus infection in the older child and adult]. | rotaviruses were detected in 1.3% of more than 10,000 stool samples from children--older than 6 years--and adults. beside rotaviruses the main enteropathogenic agents found were salmonellae (6.3%) and campylobacter jejuni/coli (1.7%). similar as the rotavirus infection of infants, in adults symptoms and signs were nearly the same except that the disease was not so severe. a correlation of the rotavirus infection with certain behaviour or eating habits was not found. | 1987 | 2830184 |
| media for isolating shigella. | 1987 | 2881104 | |
| the papg protein is the alpha-d-galactopyranosyl-(1----4)-beta-d-galactopyranose-binding adhesin of uropathogenic escherichia coli. | uropathogenic escherichia coli adhere to uroepithelial cells by their digalactoside alpha-d-galactopyranosyl-(1----4)-beta-d-galactopyranose [alpha-d-galp-(1----4)-beta-d-galp or gal alpha (1----4)gal]-binding pili, which are composed of repeating identical subunits. the major subunit (papa) of these pili is not required for binding, but the papf and papg gene products are essential for adhesion. transcomplementation analysis between the pap gene cluster and a related gene cluster encoding a dif ... | 1987 | 2886993 |
| plasmid-mediated resistance to nalidixic acid in shigella dysenteriae type 1. | in an epidemic of shigellosis in southern bangladesh the causal organism, shigella dysenteriae type 1, was resistant to nalidixic acid as well as to co-trimoxazole (trimethoprimsulphamethoxazole) and ampicillin. the genes coding for resistance to nalidixic acid, but not those coding for resistance to co-trimoxazole or ampicillin, are located on a conjugative 20 megadalton plasmid. this epidemic is of particular importance because of the resistance to nalidixic acid, an antibiotic to which shigel ... | 1987 | 2887725 |
| outbreak of shigella sonnei dysentery on a long stay psychogeriatric ward. | a ward outbreak of diarrhoea associated with shigella sonnei on a long stay psychogeriatric ward is described. nine patients and three staff had positive stool cultures for s. sonnei. the source of infection of the index case was not discovered. environmental factors at ward level which predispose long stay hospitals to such outbreaks are discussed. | 1987 | 2888815 |
| [a case of severe colitis with positive hemocultures for shigella flexneri]. | 1987 | 2954108 | |
| comparative in vitro activity of a-56268 (te-031) against gram-positive and gram-negative bacteria and chlamydia trachomatis. | the in vitro activity of a-56268 (te-031) was determined and compared with that of 13 antibiotics against 401 gram-positive and gram-negative bacteria and 11 strains of chlamydia trachomatis. a-56268 was very active against methicillin-susceptible staphylococcus aureus and neisseria gonorrhoeae, and was among the most active of the agents tested against listeria monocytogenes, streptococci and chlamydia trachomatis. it was moderately active against haemophilus spp., vibrio spp., campylobacter je ... | 1987 | 2954818 |
| [genetic characteristics and protective properties of neamin-resistant mutant salmonella typhimurium and s. dublin of the nea(r) str(s) and nea(r) str(r) 500 classes]. | the genetic analysis of attenuated mutants, class nea(r) str(s), with the use of bacteriophage p 22 has shown that mutation rendering the mutants resistant to neamine is localized in gene nea a. in experiments with the intraperitoneal infection of mice, the appearance of this mutation in s. typhimurium and s. dublin virulent strains has been found to lead to the decrease of virulence in 100% of clones. on the basis of the data obtained in this investigation, region str-spc in s. typhimurium and ... | 1987 | 2955615 |
| [shigella diagnosis--a transport problem]. | 1987 | 2957636 | |
| sequence of amino acids in lamb responsible for spontaneous ejection of bacteriophage lambda dna. | the dna sequence of the promoter-distal half of lamb from shigella sonnei 3070 has been determined and compared with the known sequence for the escherichia coli k12 gene. the only predicted amino acid changes in this region of lamb, the receptor protein for bacteriophage lambda, lie between positions 381 and 390, where seven of the ten amino acids are altered. evidence is presented that indicates that this region is responsible for the ability of the s. sonnei receptor, but not the e. coli recep ... | 1987 | 2958635 |
| an outbreak of dysentery due to shigella dysenteriae type 1 in rangoon, burma. | 1987 | 2963061 | |
| [prevalence of enteropathogenic agents in the acute diarrheal syndrome in hospitalized and outpatient children]. | 1987 | 2841726 | |
| localization of plasmid loci necessary for the entry of shigella flexneri into hela cells, and characterization of one locus encoding four immunogenic polypeptides. | we have previously cloned a 44 kb fragment from the virulence plasmid of shigella flexneri serotype 5 strain m90t which is capable of restoring invasiveness to an avirulent, plasmidless mutant. this report presents a genetic and physical analysis of tn5 mutations in recombinant clone phs4108. tn5 mutagenesis allowed identification of at least five regions implicated in the entry phenotype. these regions were located on a 20 kb portion of phs4108. expression of the insertion mutants was studied b ... | 1987 | 2846749 |
| metabolic events mediating early killing of host cells infected by shigella flexneri. | j774, a continuous macrophage cell-line, was infected by m90t, an invasive isolate of shigella flexneri serotype 5 and bs176, its non invasive derivative--which does not harbor the 220 kbase virulence plasmid pwr100. killing of host cells by intracellular m90t, commenced one hour after infection and was completed by 4 hours. intracellular bs176 did not kill cells during the same period. cell protein biosynthesis was totally inhibited by both strains within 2 hours of infection thus indicating th ... | 1987 | 2848171 |
| hypersensitivity to shigella outer membrane proteins in mice. | mice sensitized with living sh. flexneri 3a or with outer membrane proteins (omp) derived from these bacteria showed arthus and delayed type hypersensitivity (dth) when injected in the footpad with omp. the delayed reactivity to omp was transferable to normal mice by spleen cells obtained from donor animals which were previously sensitized with living bacteria. | 1987 | 2977277 |
| polymicrobial septicemia due to shigella flexneri and pseudomonas aeruginosa: first report. | an invasion of the bloodstream by members of the genus shigella is extremely rare. when it does occur, the etiologic agent is usually a single organism. a report of the first case of shigella bacteremia that was complicated by pseudomonas aeruginosa sepsis is reported. | 1987 | 3123706 |
| characterization of self-transmissible plasmids determining lactose fermentation and multiple antibiotic resistance in clinical strains of klebsiella pneumoniae. | the lactose fermentation (lac+) and antibiotic resistance (r+) phenotypes were conjugally transferred from klebsiella pneumoniae strains (k166, k182, k186, k218, and k220) to salmonella typhi, s. typhimurium, shigella flexneri, and vibrio cholerae. the genes for lactose fermentation and antibiotic resistance were located on the plasmids. further analysis of plasmid dna from these isolates indicated the presence of multiple plasmids (mr ranged less than 2.7 to 70 x 10(6)). the lac+r+ plasmids p16 ... | 1987 | 3107002 |
| multistate outbreak of shigella sonnei gastroenteritis--united states. | 1987 | 3110572 | |
| shigellosis--north carolina. | 1987 | 3110573 | |
| colicin js of shigella sonnei: classification of type colicin "7". | colicinotype 7 of shigella sonnei, introduced by abbott and graham, is one of those most frequently found in epidemiological screenings. it is represented by the production of a single colicin (acidic protein of m. w. 46 kd) endowed with some striking features. it is unstable at ph 8.0 and at the temperature of 70 degrees c. its inhibitive activity is specific for the species shigella sonnei, most strains of which seem to be sensitive. it is the only colicin known so far inactive towards escheri ... | 1987 | 3111132 |
| [improved method of lipopolysaccharide isolation from gram-negative bacteria]. | a modification of the traditional method for lipoplysaccharide isolation from the cells of grammnegative bacteria was elaborated on the basis of extraction by the hot water solution of phenol (the method of westfahl). to make the method simpler and to raise the yield of the product it was proposed to use the water-phenol extract without its division for plases. the nucleic acids are eliminated by precipitation from dialyzed extract at ph 3,2-3,4 achieved by addition of acetic acid. the comparati ... | 1987 | 3112564 |
| nationwide dissemination of multiply resistant shigella sonnei following a common-source outbreak. | 1987 | 3114612 | |
| efficacy of a polyvalent protein-polysaccharide dysentery oral vaccine. | 1987 | 3115716 | |
| [comparative in vitro activity of 10 new 4-quinolones against enteropathogenic bacteria]. | the susceptibility has been determined of 169 shigella spp., 93 salmonella spp., 68 yersinia enterocolitica, 34 vibrio cholerae 0:1, and 34 campylobacter jejuni, isolated in central africa and in belgium, to ten fluoroquinolones: ciprofloxacin, ofloxacin, norfloxacin, pefloxacin, enoxacin, amifloxacin, ci-934, a-56619, a-56620 and ro 23-6240. all quinolones were active against the strains tested, showing an mic90 between 0.004 mg/l and 2 mg/l for strains susceptible to nalidixic acid and an mic9 ... | 1987 | 3116489 |
| [effect of verapamil, cromoglycate and dimedrol on the survivability of mice in endotoxic shock]. | the trial has been performed on blocking the potentiation of the lethal action of sh. sonnei endotoxin isolated using the agents possessing antiaggregation and antimediator properties--verapamil, cromoglycate and diphenhydramine. it was shown that ld50 of endotoxin increased 2-2.5-fold, provided there were 3 drug injection 1, 4 and 9 hours after endotoxin administration. the survival of mice at 100% level of mortality was also increased 2-2.5-fold. with endotoxin administered together with the f ... | 1987 | 3118985 |
| rotaviruses in infants with diarrhea studied by viral rna electrophoresis in ankara, turkey. | incidence of rotavirus in children under 2 years of age, admitted to 2 children's hospitals i n ankara, turkey, from july 1984-june 1985 was measured using viral rna electrophoresis in agarose gels. 375 children were selected at random from over 14,000 diarrhea admissions, and their stool specimens taken on admission were compared to those of 333 children without diarrhea. 61 (16.3%) of the diarrhea cases had detectable rotavirus, compared to 0% in controls. bacteriologic tests for salmonella ... | 1987 | 2851886 |
| virulence of iron transport mutants of shigella flexneri and utilization of host iron compounds. | mutants of shigella flexneri defective in aerobactin-mediated iron transport were assayed for virulence in several model systems. a tn5 insertion mutant was invasive in hela cells, lethal in the chicken embryo, and produced keratoconjunctivitis in the guinea pig, indicating little or no loss of ability to invade and multiply intracellularly. although the mutant failed to grow in low-iron medium in vitro, growth equivalent to that of the wild type was observed in hela cell lysates. thus, there ap ... | 1987 | 3028962 |
| [gastrointestinal infections in children. a strategy for searching pathogenic agents]. | from a bacteriological and virological study of 2056 stool samples obtained from 1595 children we have attempted to derive epidemiological arguments leading to an algorithm of coprodiagnosis. we believe that rotavirus should be systematically searched for at any age, as it is the most frequent pathogen encountered (27% of children with positive detection). if the test is positive, no other test needs to be performed in patients staying less than 4 days in hospital. if the test is negative, or if ... | 1987 | 3029746 |
| in-vitro anti-microbial susceptibility of clinical isolates of pathogenic bacteria to ten antibiotics including phosphomycin. | the minimum inhibitory concentration (mic) of ten antibiotics was determined for various bacterial pathogens isolated from clinical specimens in a lagos hospital. the in-vitro activity of penicillin and tetracycline was not very impressive and a similarity was noticed in the resistance patterns of these two antibiotics, while the activity in vitro of the relatively more toxic aminoglycosides and chloramphenicol was high for gram-negative rods. ceftazidime demonstrated the highest activity in vit ... | 1987 | 3037870 |
| two-year survey of etiologic agents of diarrheal disease at san lazaro hospital, manila, republic of the philippines. | the prevalence of bacterial pathogens and rotavirus in 2,908 patients with diarrhea who were admitted to san lazaro hospital in manila in 1983 and 1984 was determined. one or more enteric pathogens were isolated or detected in samples from 1,698 (58.4%) patients. isolation rates for the various enteropathogens were as follows: rotavirus, 30.6%; shigella spp., 11.6%; salmonella spp., 9.2%; enterotoxigenic escherichia coli (1983 only), 7.8%; vibrio cholerae biotype eltor, 3.8%; non-o1 v. cholerae, ... | 1987 | 3038946 |