Publications

TitleAbstractYear(sorted ascending)
Filter
PMID
Filter
the interaction of fusarium solani pisi cutinase with long chain spin label esters.we here present a study of the interaction between the fusarium solani pisi cutinase mutant s120a and spin-labeled 4,4-dimethyloxazoline-n-oxyl-(doxyl)-stearoyl-glycerol substrates in a micellar system. the interaction is detected by nmr measuring changes in chemical shift for 1h and 15n as well as relaxation parameters for backbone 1h (t1) and 15n (t1, t2) atoms as well as for side chain methyl groups 1h (t1). the detected interaction shows a weak binding of cutinase to the lipid micelles. stru ...200616866362
[bilateral infectious keratitis in a patient wearing cosmetic soft contact lenses].we report a case of an immunocompetent 20-year-old woman, wearing planocosmetic contact lenses to change the color of her eyes, with no contact lens hygiene regimen. she developed a bilateral infectious keratitis. acanthamoeba and fusarium solani were isolated in both eyes. bilateral penetrating keratoplasty was needed because of bilateral corneal perforation. three months later, bilateral simultaneous phacoemulsification was performed because of a dense cataract and a severe decrease in her vis ...200616885898
hyperinduction of nitrilases in filamentous fungi.2-cyanopyridine proved to act as a powerful nitrilase inducer in aspergillus niger k10, fusarium solani o1, fusarium oxysporum ccf 1414, fusarium oxysporum ccf 483 and penicillium multicolor ccf 2244. valeronitrile also enhanced the nitrilase activity in most of the strains. the highest nitrilase activities were produced by fungi cultivated in a czapek-dox medium with both 2-cyanopyridine and valeronitrile. the specific nitrilase activities of these cultures were two to three orders of magnitude ...200616909267
a case of fusariosis in an immunocompromised patient successfully treated with liposomal amphotericin b.although aspergillosis remains the most common mould infection in patients with haematologic malignancies, disseminated fusarium infection is an emerging problem with a poor prognosis in this patient population. the treatment options are limited due to relative resistance of the fungus to standard antifungals. we present a patient with acute lymphoblastic leukaemia successfully treated with ambisome for a disseminated fusarium solani infection that did not respond to first line treatment with vo ...200616918066
cytotoxicity and antimicrobial activity of six multipurpose soft contact lens disinfecting solutions.to assess and compare the cytotoxicity and antimicrobial activity of six multipurpose disinfecting contact lens solutions (mpds).200616918772
the effect of additives and mechanical agitation in surface modification of acrylic fibres by cutinase and esterase.the surface of an acrylic fibre containing about 7% of vinyl acetate was modified using fusarium solani pisi cutinase and a commercial esterase, texazym pes. the effect of acrylic solvents and stabilising polyols on cutinase operational stability was studied. the half-life time of cutinase increased by 3.5-fold with the addition of 15% n,n-dimethylacetamide (dma) and by 3-fold with 1m glycerol. the impact of additives and mechanical agitation in the protein adsorption and in the hydrolysis of vi ...200616927260
systematic screening of all signal peptides from bacillus subtilis: a powerful strategy in optimizing heterologous protein secretion in gram-positive bacteria.efficient protein secretion is very important in biotechnology as it provides active and stable enzymes, which are an essential prerequisite for successful biocatalysis. therefore, optimizing enzyme-producing bacterial strains is a major challenge in the field of biotechnology and protein production. in this study, the gram-positive model bacterium bacillus subtilis was optimized for heterologous protein secretion using a novel approach. two lipolytic enzymes, cutinase from fusarium solani pisi ...200616930615
comparison of antifungal treatments for murine fusariosis.fusarium solani infections are notoriously difficult to treat. we compared the efficacy of polyenes and an echinocandin in treating murine fusariosis to identify the optimal therapeutic regimen.200616973654
[successful treatment with voriconazole for disseminated cutaneous and visceral infection by fusarium solani in a patient with acute myeloid leukemia].we report the successful treatment of a disseminated fusarium infection with skin manifestations in a severely neutropenic patient. a 51-year-old man with acute myeloblastic leukemia (m4) underwent two courses of remission induction therapy with cytarabine and daunorubicin. despite prophylactic treatment with tosufloxacin and micafungin, the patient developed a febrile scrotal ulcer. eight days later, we noted the appearance of painful and diffuse cutaneous nodules and a plain chest x-ray disclo ...200616986714
antimicrobial activity of extracts of chemical races of the lichen pseudevernia furfuracea and their physodic acid, chloroatranorin, atranorin, and olivetoric acid constituents.the antimicrobial activity and the mic values of the ethanol, chloroform, diethyl ether, and acetone extracts of the chemical races of pseudevernia furfuracea (var. furfuracea and var. ceratea) and their physodic acid, chloroatranorin, atranorin, and olivetoric acid constituents have been investigated against some microorganisms. nearly all extracts of both chemical races showed antimicrobial activity against aeromonas hydrophila, bacillus cereus, bacillus subtilis, listeria monocytogenes, prote ...200616989308
mild hydrolysis of nitriles by fusarium solani strain o1.high levels of an aromatic nitrilase (about 37 microkat/l culture) were induced in fusarium solani o1 after transfer of the mycelium from a rich medium into a medium with 20 mmol/l picolinonitrile. the mycelium was entrapped in lense-shaped particles consisting of a polyvinyl alcohol/polyethylene glycol copolymer (lentikats). the cell-free extract was immobilized by hydrophobic binding onto a butyl sepharose column. the enzyme was useful for the mild hydrolysis of nicotinonitrile, isonicotinonit ...200617007419
phase partition of gaseous hexane and surface hydrophobicity of fusarium solani when grown in liquid and solid media with hexanol and hexane.the filamentous fungus, fusarium solani, was grown in liquid and solid culture with glucose, glycerol, 1-hexanol and n-hexane. the partition coefficient with gaseous hexane (hpc) in the biomass was lower when grown in liquid medium with 1-hexanol (0.4) than with glycerol (0.8) or glucose (1) the hpc for surface growth were 0.2 for 1-hexanol, 0.5 for glycerol, 0.6 for glucose, and 0.2 for f. solani biomass obtained from a biofilter fed with gaseous n-hexane. these values show a 200-fold increase ...200617021662
members of the fusarium solani species complex that cause infections in both humans and plants are common in the environment.members of the fusarium solani species complex (fssc) are increasingly implicated as the causative agents of human mycoses, particularly in the expanding immunocompromised and immunosuppressed patient populations. best known as ubiquitous plant pathogens and saprotrophs, the fssc comprises over 45 phylogenetically distinct species distributed among three major clades. to identify which species are associated with human infections, we generated multilocus haplotypes based on four partial gene seq ...200616757619
insurgence of fusarium keratitis associated with contact lens wear.to describe the clinical presentation and course of patients who developed keratitis due to fusarium while wearing nontherapeutic soft contact lenses.200616769827
de novo design, synthesis and screening of a combinatorial library of complementary ligands directed towards the surface of cutinase from fusarium solani pisi.the protein surface is the interface through which a protein molecule senses the external world. the composition of this interface, in charged, polar and/or hydrophobic residues is crucial for both the activity and stability of the protein. protein immobilization on surfaces has been extensively explored as one of the most effective approaches for stabilization. the mechanism of stabilization, however, is still poorly understood, and usually the success of any method is more a matter of trial an ...200616779873
[clinical and microbiological study of mycetomas at the muñiz hospital of buenos aires between 1989 and 2004].this work presents clinical, microbiological and outcome data collected from 76 patients with mycetomas at the muñiz hospital from 1989 to 2004. forty-nine patients were male and 27 female; the mean age was 43.4 years. the majority of the patients acquired the infection in argentina: the most affected provinces were santiago del estero with 31 cases, and chaco with 11; 8 cases came from other countries (bolivia 6 and paraguay 2). the mean evolution of the disease was 9.2 years. the most frequent ...200616784127
studies on kinetics and thermostability of a novel acid invertase from fusarium solani.the present investigation deals with purification and thermal characterization of an acid invertase produced by fusarium solani in submerged culture. the maximum enzyme activity (9.90 u ml(-1)) was achieved after 96 h of cultivation at ph 5.0 and 30 degrees c in a basal medium containing molasses (2%) as the carbon and energy source supplemented with 1% peptone. invertase was purified by ammonium sulfate fractionation and column chromatography on deae-cellulose and sephadex g-200. the purified e ...200616787006
antibacterial, antifungal and cytotoxic properties of some sulfonamide-derived chromones.a series of antibacterial and antifungal sulfonamide (sulfanilamide, sulfaguanidine, sulfamethaxozole, 4-aminoethylbenzene-sulfonamide and 4-amino-6-trifluoromethyl-benzene-1,3-disulfonamide) derived chromones, previously reported as inhibitors of carbonic anhydrase, have been screened for in-vitro antibacterial activity against four gram-negative (escherichia coli, pseudomonas aeruginosa, salmonella typhi and shigella flexener) and two gram-positive (bacillus subtilis and staphylococcus aureus) ...200616789431
increase of the hydrophilicity of polyethylene terephthalate fibres by hydrolases from thermomonospora fusca and fusarium solani f. sp. pisi.treatment of polyethylene terephthalate fibres with hydrolase preparations from thermomonospora (thermobifida) fusca and fusarium solani f. sp. pisi resulted in an increase of the hydrophilicity of the fibres determined by measurement of their dyeing behaviour with reactive dyes and their water absorption ability. reflectance spectrometry of treated fibres dyed with a reactive dye showed that the colour became more intense corresponding to an increase of hydroxyl groups on the fibre surfaces and ...200616791721
antifungal efficacy of chitosan and its thiourea derivatives upon the growth of some sugar-beet pathogens.chitosan (cs) was modified by reaction with benzoyl thiocyanate to give a thiourea derivative (tucs). the antifungal behavior of chitosan and its thiourea derivative was investigated in vitro on the mycelial growth, sporulation and germination of conidia or sclerotia of the following sugar-beet: beta vulgaris pathogens isolated in egypt, rhizoctonia solani kühn (ag(2-2)) sclerotium rolfsii sacc. and fusarium solani (mart.) sacc. all the prepared thiourea derivatives had a significant inhibiting ...200616413607
hexadecylphosphocholine (miltefosine) has broad-spectrum fungicidal activity and is efficacious in a mouse model of cryptococcosis.the alkyl phosphocholine drug miltefosine is structurally similar to natural substrates of the fungal virulence determinant phospholipase b1 (plb1), which is a potential drug target. we determined the mics of miltefosine against key fungal pathogens, correlated antifungal activity with inhibition of the plb1 activities (plb, lysophospholipase [lpl], and lysophospholipase-transacylase [lpta]), and investigated its efficacy in a mouse model of disseminated cryptococcosis. miltefosine inhibited sec ...200616436691
herbicidal and fungicidal activities of lactones in kava (piper methysticum).this is the first report showing that kava lactones are plant and plant fungus growth inhibitors. aqueous extract of kava roots showed high allelopathic potential and strongly suppressed germination and growth of lettuce, radish, barnyardgrass, and monochoria. nine kava lactones were detected using gc-ms including desmethoxyyagonin, kavain, 7,8-dihydrokavain, hydroxykavain, yagonin, 5,6,7,8-tetrahydroxyyagonin, methysticin, dihydromethysticin, and 11-hydroxy-12-methoxydihydrokavain. quantities o ...200616448174
versatile expression and secretion vectors for bacillus subtilis.most expression systems are based on escherichia coli as the host strain because of the large availability of all kinds of vector plasmids. however, aside from the obvious advantages of e. coli systems, serious problems can occur during the process of heterologous gene expression and purification. therefore, low expression rates, formation of inclusion bodies, improper protein-folding, and/or toxicity problems might enforce changing the expression host. here we describe the construction of two n ...200616450069
deoxycholate amphotericin b and amphotericin b lipid complex exert additive antifungal activity in combination with pulmonary alveolar macrophages against fusarium solani.fusarium spp. have emerged as important causes of invasive fungal infections in immunocompromised patients. rabbit pulmonary alveolar macrophages (pams) exhibited fungicidal activity against conidia of fusarium solani and achieved a time-dependent increase in killing. neither deoxycholate amphotericin b (damb) nor amphotericin b lipid complex (ablc) exerted a suppressive effect on pams by decreasing their conidiocidal activity against f. solani. on the contrary, at a concentration of 0.125 micro ...200616466443
effect of the high polycyclic aromatic hydrocarbon, benzo[a]pyrene, on the lipid content of fusarium solani.the purpose of the present paper was to study the effect of the high polycyclic aromatic hydrocarbon (pah), benzo[a]pyrene, on the lipid [fatty acid (fa) and sterol] composition and content of the fungi fusarium solani and f. oxysporum, respectively recognized as good and poor pah degraders. the major fas and the major sterol that characterized the tested fusarium strains were c16:0, c18:1, c18:2, and ergosterol. lipid profiles of f. solani remained unchanged with the addition of benzo[a]pyrene ...200616546362
oxovanadium(iv) complexes of hydrazides: potential antifungal agents.oxovanadium(iv) -derived antifungals have been prepared by condensing equimolar amounts of vanadyl sulfate with hydrazides. all the synthesized ligands and their metal complexes were characterized by ir, uv-visible and micro analytical data. these synthesized compounds were screened for their antifungal activity against aspergillus flavus (a. flavus), trichophyton longifusus (t. longifusus), candida albicans (c. albicans), microsporum canis (m. canis), fusarium solani (f. solani) and candida gla ...200616570503
in vitro antibacterial, antifungal & cytotoxic activity of some isonicotinoylhydrazide schiff's bases and their cobalt (ii), copper (ii), nickel (ii) and zinc (ii) complexes.isonicotinoylhydrazide schiff's bases formed by the reaction of substituted and unsubstituted furyl-2-carboxaldehyde and thiophene-2-carboxaldehyde with isoniazid and, their co (ii), cu (ii), ni (ii) and zn (ii) complexes have been synthesized, characterized and screened for their in vitro antibacterial activity against mycobacterium tuberculosis, escherichia coli, klebsiella pneumoniae, proteus mirabilis, pseudomonas aeruginosa, salmonella typhi, shigella dysenteriae, bacillus cereus, corynebac ...200616570512
antifungal effects and mechanism of action of viscotoxin a3.viscotoxins are cationic proteins, isolated from different mistletoe species, that belong to the group of thionins, a group of basic cysteine-rich peptides of approximately 5 kda. they have been shown to be cytotoxic to different types of cell, including animal, bacterial and fungal. the aim of this study was to obtain information on the cell targets and the mechanism of action of viscotoxin isoform a3 (vta3). we describe a detailed study of viscotoxin interaction with fungal-derived model membr ...200616367749
sol-gel encapsulation: an efficient and versatile immobilization technique for cutinase in non-aqueous media.cutinase from fusarium solani pisi was encapsulated in sol-gel matrices prepared with a combination of alkyl-alkoxysilane precursors of different chain-lengths. the specific activity of cutinase in a model transesterification reaction at fixed water activity in n-hexane was highest for the precursor combination tetramethoxysilane/n-butyltrimetoxysilane (tmos/btms) in a 1:5 ratio, lower and higher chain lengths of the mono-alkylated precursor or decreasing proportions of the latter relative to tm ...200616095741
interactions between the soybean cyst nematode and fusarium solani f. sp. glycines based on greenhouse factorial experiments.abstract the soybean cyst nematode, heterodera glycines, and the fungus that causes sudden death syndrome (sds) of soybean, fusarium solani f. sp. glycines, frequently co-infest soybean (glycine max) fields. the interactions between h. glycines and f. solani f. sp. glycines were investigated in factorial greenhouse experiments with different inoculum levels of both organisms on a soybean cultivar susceptible to both pathogens. measured responses included root and shoot dry weights, h. glycines r ...200618943675
interaction of fusarium solani f. sp. glycines and heterodera glycines in sudden death syndrome of soybean.abstract sudden death syndrome (sds) of soybean is caused by the soilborne fusarium solani f. sp. glycines (synonym f. virguliforme). in a sequential approach, two multifactor factorial-design microplot experiments were conducted to investigate the effects of fungal infestation levels and soil moisture on both root necrosis and foliar sds severity, and the interaction between f. solani f. sp. glycines and heterodera glycines in fumigated versus nonfumigated soil. in 2003, soybean cv. spencer was ...200618943150
isolation of autochthonous non-white rot fungi with potential for enzymatic upgrading of venezuelan extra-heavy crude oil.the increasing world demand for fuels makes it necessary to exploit the largest reserve of extra-heavy crude oil (ehco) of the orinoco oil belt from venezuela. we propose the use of extracellular oxidative enzymes, in particular, lignin-degrading enzyme systems (lds) of fungi, for enzymatic improvement of ehco. autochthonous non-white rot fungal strains able to use ehco, and several polycyclic aromatic hydrocarbons (pahs) as sole carbon source and energy, were isolated from ehco-polluted soils a ...200718833334
chemical composition and antifungal activity of volatiles from three opuntia species growing in tunisia.the chemical composition of the volatiles isolated by steam distillation from leaves, flowers and fruits of opuntia lindheimeri var. linguiformis l. benson, leaves and flowers of opuntia macrorhiza engelm and leaves of opuntia microdasys (lehmann) gathered in the sea cliff of monastir town (tunisia), has been studied by gc and gc-ms. remarkable differences were noted between the composition and the constituent percentage of the different studied organs. the most important compounds found in leav ...200719070119
hypotin, a novel antipathogenic and antiproliferative protein from peanuts with a sequence similar to those of chitinase precursors.a protein designated hypotin, with both antifungal and antibacterial activity, was isolated from peanut (arachis hypogaea) seeds. the isolation procedure included extraction, ammonium sulfate precipitation, affinity chromatography on affi-gel blue gel, ion chromatography, and gel filtration. the protein exhibited a molecular mass of 30.4 kda in sds-polyacrylamide gel electrophoresis under both reducing and nonreducing conditions, indicating that it is a monomeric protein. its n-terminal sequence ...200717979231
modulation of cutinase stability and structure by phospholipid detergents.fusarium solani pisi cutinase hydrolyses triglycerides of different lengths. here we show that micelle-forming short-chain (c6-c9) phospholipids significantly reduce cutinase stability (both below and above the critical micelle concentration cmc) and rates of folding (only above cmc), trapping cutinase in an inactive state which only regains activity over hours to days, rather than the few seconds required for refolding in the absence of detergent. destabilization decreases with increasing chain ...200717980683
toll-like receptors (tlrs) expression and function in response to inactivate hyphae of fusarium solani in immortalized human corneal epithelial cells.to evaluate the role of toll-like receptors (tlrs) in host responses to fusarium solani by the use of cultured immortalized human corneal epithelial cells (hcec) and to determine whether inactive hyphal fragments can induce an antifungal response in these cells.200717982419
invasion of the french paleolithic painted cave of lascaux by members of the fusarium solani species complex.a major fungal invasion was discovered in the prehistoric painted cave of lascaux in france in sep 2001. at least three species of the fusarium solani complex were isolated and identified with a portion of the translation elongation factor 1alpha gene (ef-1alpha), a portion of the nuclear large subunit rdna (lsu) and nuclear ribosomal intergenic spacer region (its). this study represents the first time that fusarium species have been reported from a cave containing prehistoric paintings. signifi ...200718065003
[the roles of gelatinases in pathological changes of fungal keratitis in experimental rabbits].to study the roles of gelatinases, including matrix metalloproteinase (mmp)-2 and mmp-9, in pathological changes of fungal keratitis in rabbits.200718070528
in the aftermath of the fusarium keratitis outbreak: what have we learned?the 2005-2006 outbreak of fusarium keratitis associated with soft hydrophilic contact lens wear was both unprecedented and unexpected. more than 250 cases have been reported worldwide that have primarily been associated with bausch & lomb renu with moistureloc and, more recently, with bausch and lomb renu multiplus multipurpose contact lens disinfecting solutions. this article documents the outbreak, presenting the time line and the historical and scientific basis for its occurrence. underlying ...200719668512
antifungal activity and enhancement of plant growth by bacillus cereus grown on shellfish chitin wastes.bacillus cereus qq308 produced antifungal hydrolytic enzymes, comprising chitinase, chitosanase and protease, when grown in a medium containing shrimp and crab shell powder (scsp) produced from marine waste. the growth of the plant-pathogenic fungi fusarium oxysporum, fusarium solani, and pythium ultimum were considerably affected by the presence of the qq308 culture supernatant. the supernatant inhibited spore germination and germ tube elongation of f. oxysporum, f. solani, and p. ultimum. the ...200716797180
modification of phenolic metabolism in soybean hairy roots through down regulation of chalcone synthase or isoflavone synthase.soybean hairy roots, transformed with the soybean chalcone synthase (chs6) or isoflavone synthase (ifs2) genes, with dramatically decreased capacity to synthesize isoflavones were produced to determine what effects these changes would have on susceptibility to a fungal pathogen. the isoflavone and coumestrol concentrations were decreased by about 90% in most lines apparently due to gene silencing. the ifs2 transformed lines had very low ifs enzyme activity in microsomal fractions as measured by ...200716924535
the role of chitosan in protection of soybean from sudden death syndrome caused by fusarium solani f. sp. glycines.the in vitro antifungal properties of chitosan and its role in protection of soybean from a sudden death syndrome (sds) were evaluated. chitosan inhibited the radial and submerged growth of f. solani f. sp. glycines with a marked effect at concentrations up to 1mg/ml indicating antifungal property and at 3mg/ml was able to delay sds symptoms expression on soybean leaves for over three days after fungal inoculation when applied preventively. chitosan was able to induce the level of chitinase acti ...200716828285
production of fusarium solani f. sp. pisi cutinase in fusarium venenatum a3/5.fusarium venenatum a3/5 was transformed using the aspergillus niger expression plasmid, pigf, in which the coding sequence for the f. solani f. sp. pisi cutinase gene had been inserted in frame, with a kex2 cleavage site, with the truncated a. niger glucoamylase gene under control of the a. niger glucoamylase promoter. the transformant produced up to 21 u cutinase l(-1) in minimal medium containing glucose or starch as the primary carbon source. glucoamylase (165 u l(-1) or 8 mg l(-1)) was also ...200717505784
expression of matrix metalloproteinases 2 and 9 in experimental corneal injury and fungal keratitis.levels of matrix metalloproteinases (mmps) can be modulated during corneal infection, but little is known about mmp profiles during fungal keratitis. the purpose of this study was to determine the effect of corneal trauma and immunosuppressive treatment on the expression kinetics of mmp-2 and mmp-9 during experimental keratomycosis.200717525657
fungal neuroinfections: rare disease but unacceptably high mortality.within last 25 years we have observed 20 cases of fungal meningitis and/or cerebral abscesses. commonest etiologic agens was candida spp. (c. albicans 9 of 20). molds were responsible for 4 cases of brain abscess. mortality was 50% what seems to be very high. extremely high mortality is caused by delayed onset of therapy, severe underlying disease and multiresistant fungal organisms such as mucorales, fusarium solani and aureobasidium.200717558372
expression of a ribosome-inactivating protein gene in bitter melon is induced by sphaerotheca fuliginea and abiotic stimuli.the gene encoding a single-chain, ribosome-inactivating protein (scrip) was cloned from bitter melon (momordica charantia l.) leaves infected with the fungus, sphaerotheca fuliginea, by rt-pcr. the orf was 861 bp. the ribosome-inactivating protein was expressed in e. coli and, when purified, it inhibited the growth of fusarium solani [corrected] northern blot analysis revealed that rip transcripts rapidly accumulated in leaves 1-day post inoculation with sphaerotheca fuliginea and reached a peak ...200717579811
efficacy of topical caspofungin in experimental fusarium keratitis.to evaluate the efficacy of caspofungin in an experimental rabbit model of fusarium keratitis and to compare it with amphotericin b.200717592325
in vitro antifungal activity of rhazya stricta.rhazya stricta is a small glabrous shrub, widely distributed throughout western asia from yemen to arabia, to the north west province of india and abundantly found in various regions of pakistan. larvicidal and antifungal studies of polar and non polar aerial parts extracts of rhazya stricta were performed using brine shrimps larvae for larvicidal study and for antifungal study microorganisms, trichophyton longifusis, aspergillus flavus, candida albicans, microsporum canis and fusarium solani we ...200717604249
[experimental study of fast diagnosis of mycotic keratitis using molecular biology technical].to establish a method for fast diagnosis of mycotic keratitis using multi-pcr system.200717605910
comparison of pathogen-induced expression and efficacy of two amphibian antimicrobial peptides, msra2 and temporin a, for engineering wide-spectrum disease resistance in tobacco.the rapid accumulation of defensive transgene products in plants only on pathogen invasion has clear advantages over their constitutive synthesis. in this study, two antimicrobial peptides from the skin secretions of frogs, msra2 (n-methionine-dermaseptin b1) and temporin a, were evaluated for engineering pathogen-induced disease resistance in plants. both peptides inhibited plant-specific pathogens in vitro at micromolar concentrations that were not toxic to plant protoplasts. the plant-optimiz ...200717645440
interaction of fusarium solani lectin with monosaccharides and oligosaccharides: a fluorometric study.the intrinsic fluorescence intensity of fusarium solani lectin was quenched upon binding to mono- and oligosaccharides without any change in the emission maximum and it was used to determine association constants for several sugars and glycans. the lectin interacted very poorly with monosaccharides but well with disaccharides (t-antigen and lacnac) with a distinction between beta1-->4 and beta1-->3 linkages. among the monosaccharides, the interaction was observed only with gal/galnac derivatives ...200717645671
toxigenic fungi and mycotoxins in mature corn silage.to investigate the exposure of livestock and farm workers to mycotoxins during the last months of silage use, the mycoflora and the mycotoxins in a mature silage (11-months-old) were studied. a multimycotoxin method was developed to evaluate the toxigenic in vitro ability of fungal strains. the screening of potentially toxigenic fungi isolated from the mature silage showed that six fusaria (fusarium culmorum, fusarium equiseti, fusarium graminearum, fusarium oxysporum, fusarium solani and fusari ...200717655998
modeling hydration mechanisms of enzymes in nonpolar and polar organic solvents.a comprehensive study of the hydration mechanism of an enzyme in nonaqueous media was done using molecular dynamics simulations in five organic solvents with different polarities, namely, hexane, 3-pentanone, diisopropyl ether, ethanol, and acetonitrile. in these solvents, the serine protease cutinase from fusarium solani pisi was increasingly hydrated with 12 different hydration levels ranging from 5% to 100% (w/w) (weight of water/weight of protein). the ability of organic solvents to 'strip o ...200717419728
prediction of retention time of cutinases tagged with hydrophobic peptides in hydrophobic interaction chromatography.hydrophobic interaction chromatography (hic) is an important technique for protein purification, which exploits the separation of proteins based on hydrophobic interactions between the stationary phase ligands and hydrophobic regions on the protein surface. one way of enhancing the purification efficiency by hic is the addition of short sequences of peptide tags to the target protein by genetic engineering, which could reduce the need for extra and expensive chromatographic steps. in the present ...200717448484
evaluation of the inhibitory effect of amphotericin b on the apical growth of f. solani using the biocell-tracer system.the biocell-tracer (bct) system is an automatic microscopic method used for measuring the growth rate of a single fungal hyphae, which has not yet been applied to study fusarium spp. considering the large resistance of fusarium species to the available chemotherapy and that hyphae is the morphological fungal form most often seen in vivo, in this work, amphotericin b mic and mfc values for a fusarium solani strain were obtained by the conventional assay method testing conidia and also by the bct ...200717472613
quality control and reference guidelines for clsi broth microdilution method (m38-a document) for susceptibility testing of anidulafungin against molds.the clsi (formerly nccls) m38-a document for antifungal susceptibility testing of filamentous fungi does not describe guidelines for echinocandins. a multicenter study (eight centers) evaluated inter- and intralaboratory reproducibilities of two reading times (24 and 48 h or 48 and 72 h) and two end points (mics and minimum effective concentrations [mecs]) for evaluating anidulafungin against molds. anidulafungin mics (>or=50% inhibition) and mecs (morphological hyphal changes) were determined f ...200717475760
biotransformation of heterocyclic dinitriles by rhodococcus erythropolis and fungal nitrilases.2,6-pyridinedicarbonitrile (1a) and 2,4-pyridinedicarbonitrile (2a) were hydrated by rhodococcus erythropolis a4 to 6-cyanopyridine-2-carboxamide (1b; 83% yield) and 2-cyanopyridine-4-carboxamide (2b; 97% yield), respectively, after 10 min. after 118 h, the intermediates 1b or 2b were transformed into 2,6-pyridinedicarboxamide (1c; 35% yield) and 2,6-pyridinedicarboxylic acid (1d; 60% yield) or 2-cyanopyridine-4-carboxylic acid (2c; 64% yield), respectively. the nitrilase from fusarium solani af ...200717479225
chemical modification results in hyperactivation and thermostabilization of fusarium solani glucoamylase.chemical modification of carboxyl groups of glucoamylase from a mesophilic fungus, fusarium solani, was carried out using ethylenediamine as nucleophile in the presence of water-soluble 1-ethyl-3(3-dimethylaminopropyl)carbodiimide. modification brought about a dramatic enhancement of catalytic activity and thermal stability of glucoamylase. temperature and ph optima of ethylenediamine-coupled glucoamylase (ecg) increased as compared with those of native enzyme. the specificity constant (k(cat)/k ...200717496965
outbreak of keratomycosis attributable to fusarium solani in the french west indies.we report an unusual increase in the number of cases of keratomycosis attributable to fusarium solani in contact lens wearers.200717258535
molecular characterization, biofilm analysis and experimental biofouling study of fusarium isolates from recent cases of fungal keratitis in new york state.to characterize fusarium isolates from recent cases of fungal keratitis in contact lens wearers, and to investigate fungal association with moistureloc solution.200717263885
universal in vitro antifungal resistance of genetic clades of the fusarium solani species complex.eleven antifungal drugs were tested against representative isolates of the four phylogenetic clades of the fusarium solani species complex obtained in a multilocus sequence analysis. they all showed very poor activity, with no differences among the clades. amphotericin b was the most active drug.200717220423
effects of a chitin-binding vicilin from enterolobium contortisiliquum seeds on bean bruchid pests (callosobruchus maculatus and zabrotes subfasciatus) and phytopathogenic fungi (fusarium solani and colletrichum lindemuntianum).chitin-binding vicilin from enterolobium contortisiliquum seeds was purified by ammonium sulfate followed by gel filtration on sephacryl 300-sh and on sephacryl 200-sh. the vicilin, called ecv, is a dimeric glycoprotein composed of 1.03% carbohydrates and a mr of 151 kda, consisting of two subunits of mr of 66.2 and 63.8 kda. the ecv homogeneity was confirmed in a page where it was observed to be a unique acid protein band with slow mobility in this native gel. e. contortisiliquum vicilin (ecv) ...200717227051
purification and characterization of a lectin from endophytic fungus fusarium solani having complex sugar specificity.a lectin from the mycelial extract of an endophytic strain of fusarium solani was purified. its hemagglutinating activity was inhibited by glycoproteins possessing n-linked as well as o-linked glycans. the thermodynamics and kinetics of binding of glycans and glycoproteins to f. solani lectin was studied using surface plasmon resonance. the lectin showed high affinity for asialofetuin, asialomucin, asialofibrinogen, and thyroglobulin; and comparatively low affinity for mucin, fetuin, fibrinogen, ...200717118333
comparison of the hydrolysis of polyethylene terephthalate fibers by a hydrolase from fusarium oxysporum lch i and fusarium solani f. sp. pisi.the hydrolysis of polyethylene terephthalate (pet) fibers by two fungal hydrolases was investigated. the hydrolase from a newly isolated fusarium oxysporum strain (lch 1) was more efficient in releasing terephthalic acid from pet fibers compared to the enzyme from f. solani f. sp. pisi dsm 62420 when equal amounts of p-nitrophenyl butyrate-hydrolyzing activity were employed. pet fabrics treated under the same conditions with the enzyme from f. oxysporum lch 1 also showed a considerably higher in ...200717136729
deep cutaneous infection by fusarium solani in a healthy child: successful treatment with local heat therapy.we describe a 6-year-old boy with a granulomatous lesion on the upper aspect of his left arm. fusarium solani infection was confirmed by biopsy studies and cultures. the biopsy specimen showed an unusually extensive dermal invasion with fungal hyphae. this is an uncommon clinical presentation for deep cutaneous infection caused by f solani in a healthy child. local heat therapy with a chemical pocket warmer led to dramatic healing of the lesion.200717147970
in vivo confocal microscopy in fungal keratitis.fungal keratitis is a major blinding eye disease found throughout the world, particularly in developing countries. given the recent increase in fusarium keratitis infections in contact lens wearers owing to contact lens solutions, a warning was recently issued by the food and drug administration, making it a public health concern in developed countries.200717151059
effect of aniline coupling on kinetic and thermodynamic properties of fusarium solani glucoamylase.purified glucoamylase (ga) from fusarium solani was chemically modified by cross-linking with aniline hydrochloride in the presence of 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (edc) for 1 [aniline-coupled glucoamylase-1 (acg-1)], 7 (acg-7), and 13 min (acg-13). the aniline coupling of ga had a profound enhancing effect on temperature, ph optima, and pk (a)'s of active site residues. the specificity constants (k (cat)/k (m)) of native, acg-1, acg-7, and acg-13 were 136, 244, 262, and 208 at ...200717031637
in vitro and in vivo antifungal activity of cetrimide (cetyltrimethyl ammonium bromide) against fungal keratitis caused by fusarium solani.mycotic keratitis is a devastating eye infection acquired after eye injury. cetrimide at 15 and 20 mg ml(-1) produced no surviving fusarium solani growth with minimal inhibitory concentration value of 0.10 mg ml(-1). topical administration of three drops (0.3 ml) of cetrimide aqueous solution of 10 mg ml(-1) at ph 6.4 three times daily succeeded to cure human severe resistant f. solani keratitis in a time course of <3 weeks, and with complete healing after 6 weeks. cetrimide-treated rabbit corne ...200717302751
tailoring cutinase activity towards polyethylene terephthalate and polyamide 6,6 fibers.cutinase from fusarium solani pisi was genetically modified near the active site, by site-directed mutagenesis, to enhance its activity towards polyethylene terephthalate (pet) and polyamide 6,6 (pa 6,6) fibers. the mutations l81a, n84a, l182a, v184a and l189a were done to enlarge the active site in order to better fit a larger polymer chain. modeling studies have shown enhanced free energy stabilization of model substrate tetrahedral intermediate (ti) bound at the enzyme active site for all mut ...200717306400
chemical, thermal and ph-induced equilibrium unfolding studies of fusarium solani lectin.the effect of urea, guanidine thiocyanate, temperature and ph was studied on the conformational stability of fusarium solani lectin. equilibrium unfolding with chemical denaturants showed that the lectin was least stable at ph 12 and maximally stable at ph 8.0 near its pi (8.7). guanidine thiocyanate (the concentration of denaturant at which the protein is half folded, d1/2 = 0.49 m at ph 12) was found to be an eight times stronger denaturant than urea (d1/2 = 3.88 m at ph 12). the unfolding cur ...200717365178
attachment to and penetration of conventional and silicone hydrogel contact lenses by fusarium solani and ulocladium sp. in vitro.to analyze the relative capacities of fusarium solani and ulocladium sp. to attach to and penetrate silicone hydrogel soft contact lenses.200717667618
[the effect of infection by fusarium solani f. sp. batatas on endogenous hormone levels in sweet potato seedling].the endogenous hormone aba concentrations increased in the leaves, shoots and roots of sweet potato plants infected with fusarium solani f. sp. batatas (fsb) or treated by its culture filtrate. the accumulation of aba occurred firstly in the roots, but the highest concentration was in the shoots. the endogenous ga(1/3) concentration of leaves, shoots and roots of infected sweet potato kept at markedly low levels during the period of experiments.the aba concentration of sweet potato seedlings inc ...200717675755
fusarium solani species complex isolates conspecific with fusarium solani f. sp. cucurbitae race 2 from naturally infected human and plant tissue and environmental sources are equally virulent on plants, grow at 37 degrees c and are interfertile.in a previous taxonomic study based on multilocus sequencing of fusarium from clinical specimens and hospital environments, the most common lineage was fusarium solani species complex group 1 (fssc 1) which is conspecific with f. solani f. sp. cucurbitae race 2, a pathogen of cucurbit fruits. the aims of our study were to determine if clinical and environmental isolates of fssc 1 are plant pathogens and members of the same biological species as cucurbit isolates, and to determine if all isolates ...200717686017
dna microarray-based detection and identification of fungal pathogens in clinical samples from neutropenic patients.the increasing incidence of invasive fungal infections (ifi) in immunocompromised patients emphasizes the need to improve diagnostic tools. we established a dna microarray to detect and identify dna from 14 fungal pathogens (aspergillus fumigatus, aspergillus flavus, aspergillus terreus, candida albicans, candida dubliniensis, candida glabrata, candida lusitaniae, candida tropicalis, fusarium oxysporum, fusarium solani, mucor racemosus, rhizopus microsporus, scedosporium prolificans, and trichos ...200717715373
antimicrobial activity of methanol extract of origanum majorana l. (sweet marjoram).in-vitro microbicidal activity of the methanol extract of origanum majorana l. was tested against seven fungi (fusarium solani, candida albicans, aspergillus niger, a. parasiticus, rhizopus oryzae, rhizoctonia otyzae-sativae and altemaria brassicicola) and six bacteria (bacillus subtilis, b. megaterium, escherichia coil, proteus vulgaris, pseudomonas aeruginosa and staphylococcus aureus). the methanol extract of o. majorana can be used as an effective herbal protectant against different pathogen ...200717718003
ulcerous lesions disclosing cutaneous infection with fusarium solani.fusarium spp. are emergent opportunistic moulds capable of producing life-threatening diseases, usually in immunocompromised hosts. when they infect immunocompetent patients, they usually cause localized diseases. we describe here a case of cutaneous fusariosis in a patient with a medical history of diabetes mellitus and non-hodgkin's lymphoma who presented with two painful lesions on her left leg. the diagnosis was made on the basis of histopathological findings and skin biopsy culture. the les ...200717721650
deep cutaneous infection by fusarium solani in a healthy child. 200718021857
isolation and biochemical characterization of an antifungal peptide from amaranthus hypochondriacus seeds.an antifungal peptide, ay-amp, was isolated from amaranthus hypochondriacus seeds by acidic extraction and then purified by reverse-phase high-pressure liquid chromatography. the molecular mass of this peptide, as determined by mass spectrometry, is 3184 da. the peptide belongs to the superfamily of chitin-binding proteins, containing a single cysteine/glycine-rich chitin-binding domain, and it was found that ay-amp degrades chitin. ay-amp inhibits the growth, at very low doses, of different pat ...200718031007
role of ethylene in the protection of tomato plants against soil-borne fungal pathogens conferred by an endophytic fusarium solani strain.an endophytic fungal isolate (fs-k), identified as a fusarium solani strain, was obtained from root tissues of tomato plants grown on a compost which suppressed soil and foliar pathogens. strain fs-k was able to colonize root tissues and subsequently protect plants against the root pathogen fusarium oxysporum f.sp. radicis-lycopersici (forl), and elicit induced systemic resistance against the tomato foliar pathogen septoria lycopersici. interestingly, attenuated expression of certain pathogenesi ...200718048373
in vitro interactions of antifungal agents against clinical isolates of fusarium spp.the in vitro activities of amphotericin b (amb), itraconazole (itc), voriconazole (vcz) and terbinafine (tbf) alone and in the combinations amb+vcz, tbf+itc and tbf+vcz were evaluated against 29 clinical isolates of fusarium spp. (15 fusarium solani, 7 fusarium oxysporum, 2 fusarium decemcellulare, 2 fusarium dimerum and 3 other fusarium spp.). minimum inhibitory concentrations were determined using the method of the clinical and laboratory standards institute and the interaction activity was ca ...200818054469
antifungal activity of chitinases from trichoderma aureoviride dy-59 and rhizopus microsporus vs-9.two chitinolytic fungal strains, trichoderma aureoviride dy-59 and rhizopus microsporus vs-9, were isolated from soil samples of korea and vietnam, respectively. dy-59 and vs-9 crude chitinases secreted by these fungi in the 0.5% swollen chitin culture medium had an optimal ph of 4 and the optimal temperatures of 40 degrees c and 60 degrees c, respectively. enzymatic hydrolysis products from crab swollen chitin were n-acetyl-beta-d-glucosamine (glcnac) by dy-59 chitinase, and glcnac and n, n'-di ...200817896135
antifungal efficacy of voriconazole, itraconazole and amphotericin b in experimental fusarium solani keratitis.to evaluate the efficacy of topical voriconazole in an experimental rabbit model of fusarium keratitis.200817912543
sewage and community shower drains are environmental reservoirs of fusarium solani species complex group 1, a human and plant pathogen.in two recent studies, clinical isolates in the fusarium solani species complex (fssc) were sequenced; one of the most common lineages was fssc group 1 (fssc 1), a phylogenetic species that is synonymous with f. solani f. sp. cucurbitae race 2, a pathogen of cucurbit fruits. fssc 1 was also identified in sink and shower drains in two hospitals. the environmental sources of fssc 1 are important for understanding the epidemiology of both human and plant diseases caused by this organism. fssc 1 was ...200817916080
comparing the effect of immobilization methods on the activity of lipase biocatalysts in ester hydrolysis.the activity of various lipases was compared, in both free and immobilized forms, using the kinetics of the hydrolysis reaction of p-nitrophenyl butyrate, which was followed with in situ uv/vis diode array spectrophotometry. several enzymes were used to catalyze the reaction, namely candida antarctica lipase b and fusarium solani pisi cutinase wildtype and three single-mutation variants. the enzymes were tested in three different forms: free, immobilized as cross-linked aggregates and supported ...200817940805
rational design of a new one-step purification strategy for candida antarctica lipase b by ion-exchange chromatography.a fast and efficient one-step method for purification of lipase b from candida antarctica by ion-exchange chromatography was developed by rational design. the electrostatic properties of the enzyme were calculated and validated by isoelectric focusing and measurement of the titration curve. c. antarctica lipase b shows an unusual ph profile with a broad isoelectric region from ph 4 to 8. at ph 3 c. antarctica lipase b can be bound to a cation-exchange chromatography column and was purified to ho ...200818154980
dna microarray based on arrayed-primer extension technique for identification of pathogenic fungi responsible for invasive and superficial mycoses.an oligonucleotide microarray based on the arrayed-primer extension (apex) technique has been developed to simultaneously identify pathogenic fungi frequently isolated from invasive and superficial infections. species-specific oligonucleotide probes complementary to the internal transcribed spacer 1 and 2 (its1 and its2) region were designed for 24 species belonging to 10 genera, including candida species (candida albicans, candida dubliniensis, candida famata, candida glabrata, candida tropical ...200818160452
occupational hypersensitivity pneumonitis due to molds in an onion and potato sorter.a 42-year-old female non-smoking onion and potato sorter developed work-related shortness of breath, cough, fatigue and flu-like symptoms. the diagnosis of hypersensitivity pneumonitis was based on patchy infiltrates in both lungs on high resolution computed tomography and lymphocytosis of 71% in a bronchoalveolar lavage sample with a cd4/cd8 ratio of 0.4. exposure cessation and initial corticosteroid therapy resulted in complete recovery. igg antibodies to penicillium species and fusarium solan ...200818161826
interactions between glyphosate and autochthonous soil fungi surviving in aqueous solution of glyphosate.the survival of autochthonous fungi in soil treated with 1mm aqueous solution of glyphosate was investigated. significant differences in the total number of fungi in the studied objects were observed, and additionally significant qualitative changes were encountered. the dominating group of fungi belonged to genus fusarium: fusarium solani h30, fusarium solani h50 and fusarium oxysporum h80. interactions between the isolated strains of fungi and varying concentrations of glyphosate were determin ...200818177917
activities of voriconazole, itraconazole and amphotericin b in vitro against 590 moulds from 323 patients in the voriconazole phase iii clinical studies.fungal pathogens from the voriconazole trials were identified and tested for susceptibility at two reference laboratories.200818222957
fungal root endophytes from natural vegetation in mediterranean environments with special reference to fusarium spp.surveys (in 2002 and 2003) were performed for fungal endophytes in roots of 24 plant species growing at 12 sites (coastal and inland soils, both sandy soils and salt marshes) under either water or salt stress in the alicante province (southeast spain). all plant species examined were colonized by endophytic fungi. a total of 1830 fungal isolates were obtained and identified by morphological and molecular [internal transcribed spacer (its) and translation elongation factor-1alpha gene region (tef ...200818248439
antifungal susceptibility profile of clinical fusarium spp. isolates identified by molecular methods.to analyse the susceptibility pattern of a collection of fusarium clinical isolates.200818263569
protein structure and dynamics in ionic liquids. insights from molecular dynamics simulation studies.we present in this work the first molecular simulation study of an enzyme, the serine protease cutinase from fusarium solani pisi, in two ionic liquids (ils): 1-butyl-3-methylimidazolium hexafluorophosphate ([bmim][pf6]) and 1-butyl-3-methylimidazolium nitrate ([bmim][no(3)]). we tested different water contents in these ils at room temperature (298 k) and high temperature (343 k), and we observe that the enzyme structure is highly dependent on the amount of water present in the il media. we show ...200818266354
amphotericin b formulations variably enhance antifungal activity of human neutrophils and monocytes against fusarium solani: comparison with aspergillus fumigatus.lipid formulations of amphotericin b (ambf) are widely used in the treatment of life-threatening infections caused by aspergillus fumigatus and fusarium solani. we aimed to compare the immunomodulatory effects of four ambf, deoxycholate (damb), liposomal (lamb), lipid complex (ablc) and colloidal dispersion (abcd), on the oxidative antifungal activities of human neutrophils (pmns) and monocytes (mncs) against hyphae of a. fumigatus and f. solani.200818272514
orientation of cutinase adsorbed onto pmma nanoparticles probed by tryptophan fluorescence.the fluorescence of the single tryptophan (trp69) of cutinase from fusarium solani pisi, free in aqueous solution and adsorbed onto the surface of poly(methyl methacrylate) (pmma) latex particles, was studied at phs of 4.5 and 8.0. the monodisperse pmma particles (d=106.0+/-0.1 nm) were coated with a quite compact monolayer of cutinase at both ph values. the trp decay curve of the folded free cutinase in solution can only be fitted with a sum of four exponentials with lifetimes of 0.05, 0.3-0.4, ...200818311968
expression in escherichia coli, purification, refolding and antifungal activity of an osmotin from solanum nigrum.abstract:200818334031
[non-dermatophytic moulds: onychomycosis in four patients infected with the human immunodeficiency virus].patients infected with human immunodeficiency virus (hiv) are a risk group for onychomycosis, fungal infections caused mainly by dermatophytes and yeast. however, non-dermatohytic moulds are becoming common agents for nail infections in this population of patients. we report four cases of onychomycosis caused by non-dermatophytic moulds (aspergillus niger, scytalidium hyalinum, scytalidium dimidiatum and fusarium solani) in patients infected with hiv from recife, pernambuco, brazil. onychomicosi ...200818338928
antifungal and antibacterial activities of taxus wallichiana zucc.current study was undertaken to evaluate the in vitro antifungal and antibacterial potential of methanol extract and subsequent fractions obtained after partitioning in organic solvents with variable polarity of the aerial parts of the tree taxus wallichiana zucc. traditionally, this plant is often used in folk medicines in pakistan for treating microbial infections. in order to rationalize the traditional use, methanol extracts of leaf, bark, and heartwood of taxus wallichiana zucc. were tested ...200818343912
fungal removal of gaseous hexane in biofilters packed with poly(ethylene carbonate) pine sawdust or peat composites.the removal of volatile organic compounds (voc) in biofilters packed with organic filter beds, such as peat moss (pm) and pine sawdust (ps), frequently presents drawbacks associated to the collapse of internal structures affecting the long-term operation. poly(ethylene ether carbonate) (peec) groups grafted to these organic carriers cross linked with 4,4'-methylenebis(phenylisocyanate) (mdi) permitted fiber aggregation into specific shapes and with excellent hexane sorption performance. modified ...200818351679
[diversity of facultatively anaerobic microscopic mycelial fungi in soils].the numbers of microscopic fungi isolated from soil samples after anaerobic incubation varied from tens to several hundreds of cfu per one gram of soil; a total of 30 species was found. this group is composed primarily of mitotic fungi of the ascomycete affinity belonging to the orders hypocreales (fusarium solani, f. oxysporum, fusarium sp., clonostachys grammicospora, c. rosea. acremonium sp., gliocladium penicilloides, trichoderma aureoviride, t. harzianum, t. polysporum, t. viride. t. koning ...200818365728
fusarium solani in the post-transplant patient: an unusual fungus.the incidence of fungal infections in post-transplant patients ranges from 2% to 50%, and more uncommon pathogens are being isolated. earlier, the fungi seen were candida, aspergillus, and cryptococcus; and of late, histoplasma, coccidiodomycoses, and dematiaceous fungi are seen. in this article, a patient with invasive fusariosis is presented, and literature of this uncommon fungus is reviewed.200818372269
Displaying items 601 - 700 of 1378