Publications

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the production of high titre poliovirus in concentrated suspensions of tissue culture cells. 196013844202
a simple method for concentration of live and formaldehyde-inactivated poliovirus. 196013829716
thermal inactivation of mefl poliovirus. 196013824872
ultraviolet inactivation of poliovirus ribonucleic acid. 196014427608
quantitative studies on the infectivity of ribonucleic acid from partially purified and highly purified poliovirus preparations. 196014410319
the use of attenuated poliovirus in an epidemic area, managua, nicaragua, 1958. 196013856129
a simple test for serodifferentiation of poliovirus strains within the same type. 196013843361
[study of the glutamic-oxalacetic transaminase activity in kb cells in normal culture and after poliovirus inoculation]. 196013827694
destruction of poliovirus in the digestive tract of chimpanzees by means of specific high-titre gamma-globulin. 196014407261
[infection by poliovirus in pregnancy]. 196013837498
the case for live poliovirus vaccination. 196014430990
the effect of x-irradiation on the yield of poliovirus. 196014416296
a review of theoretical, experimental, and practical considerations in the use of formaldehyde for the inactivation of poliovirus. 196014441021
inactivation of concentrated purified poliovirus suspensions. 196013809340
poliovirus vaccines, killed and live. 196014430989
a simplified procedure for purification of large amounts of poliovirus: characterization and amino acid analysis of type 1 poliovirus. 196014416320
large scale field trial with sabin's live poliovirus vaccine in czechoslovakia. 196013831540
poliovirus protein: source of amino acids and time course of synthesis. 196013814143
repeated serological surveys performed in the general population of czechoslovakia before and after the vaccination of children with inactivated and live poliovirus vaccine. 196013787596
[studies on the diagnostic value of the complement fixation reaction with poliovirus antigens]. 196013773481
effects of rapid mass immunization of a population with live, oral poliovirus vaccine under conditions of massive enteric infection with other viruses. 196013745165
the genetic relationship between thermostability of poliovirus and its in vivo response to cystine. 196013724572
the biosynthesis of poliovirus in cell cultures. 196013719801
differences in chromatographic behavior on cellulose columns of virulent mahoney and attnuated lsc strains of poliovirus and similarities of base ratios of their nucleic acids. 196013715366
vaccination with the chat strain of type 1 attenuated poliomyelitis virus in leopoldville. belgian congo. 2. studies of the safety and efficacy of vaccination.in the course of the attenuated live poliovirus vaccination trial described in the preceding paper, an estimated 46 000 african children were given chat strain. among the 3400 children followed up by home visits, none developed paralytic poliomyelitis or aspetic meningitis. when bled 2-3 months after vaccination, 60% of previously type-1 negative children had antibodies; interference by wild enteric viruses is suggested in explanation of this low figure.two months after the beginning of vaccinat ...196014433516
studies on the development of natural immunity to poliomyelitis in louisiana. vi. the incidence of poliovirus infections during 1958 as an indication of the effect of salk-type vaccine on virus dissemination. 196014434441
[prolonged elimination by the feces of type ii poliomyelitis virus (lansing) in a convalescent]. 196014441351
the behavior of different strains of poliomyelitis virus in the complement fixation reaction. ii. the sensitivity and specificity of antigens derived from various strains. 196014443079
the behavior of different strains of poliomyelitis virus in the complement fixation reaction. iii. comparison of the potency of complement fixing antigens derived from different strains of poliomyelitis virus. 196014443080
virus production by subcultured monkey-kidney cells.cultured monkey-kidney cells, like the rabbit cells previously studied, have a relatively high folic acid requirement for growth. subcultures can be readily prepared if the medium is supplemented with a high level of the vitamin. the sensitivity of the resultant cells to poliovirus and the yields of virus obtainable with them are the same as with primary cultures.196014416839
poliovirus inhibitor from the central nervous system of the rhesus monkey.suspensions from the central nervous system of rhesus monkeys inhibited the infection of monkey kidney cell cultures by types 1, 2, and 3 poliovirus, whereas inhibition of coxsackie a9 and echo 12 viruses could not be readily demonstrated. failure of suspensions of tissues of the central nervous system to irreversibly neutralize poliovirus indicated that the inhibition was not directed against the virus but affected viral multiplication by altering the host cells.196014418520
vaccination with the chat strain of type 1 attenuated poliomyelities virus in leopoldville, belgian congo. 1. description of the city, its history of poliomyelitis, and the plan of the vaccination campaign.a trial of the chat, type 1, attenuated poliovirus strain of koprowski was begun in august 1958 in léopoldville, a city of 350 000 people, and possessing modern medical facilities, including an institute of public health.paralytic poliomyelitis is endemic in the city; since 1951 there has been an average annual incidence of 63 cases, and a rate of 19.4 cases per 100 000 inhabitants. more than 80% of the cases have occurred in african children less than 3 years old. consequently, a plan to vaccin ...196014415050
neurovirulence of attenuated type 1 poliovirus vaccine strains after intramuscular inoculation of rhesus monkeys. 196014409387
human and animal antibody responses to commercial poliomyelitis vaccine.the evaluation of the poliomyelitis vaccine field trials conducted in the usa in 1954 showed that there was considerable variation in the antigenicity of the vaccines then in use. since then, however, there have been changes both in manufacturing procedures and in immunization schedules; and a new study has therefore been conducted into the antibody response of triply negative children, aged 6months to 3 years, to commercial vaccine. the results show good response for all three poliovirus types ...196014410625
genetic markers and serological identity of wild and attenuated strains of type-1 poliovirus, with special emphasis on strains isolated from patients during an epidemic in the belgian congo.an epidemic of paralytic poliomyelitis occurred in léopoldville at a time when both virulent and attenuated type 1 polioviruses were present in the population. the attenuated virus was of the chat strain, then being given extensive field trials as a vaccine in the belgian congo. it was thought that intratypic identification of "wild" and vaccine strains might give information on the hypothetical possibility of a reversion to virulence of attenuated strains. viruses were isolated from the stools ...196014410972
a physical property as a virus marker. difference in avidity of cellulose resin for virulent (mahoney) and attenuated (lsc, 2ab) strain of type 1 poliovirus. 196014402217
immunity to poliomyelitis in guatemala: a serological and virological survey.a mass poliomyelitis vaccination campaign, such as that under consideration in guatemala, inevitably changes the immunological picture of a population by the artificial stimulation of antibodies in large numbers of individuals. it was therefore decided that before carrying out such a campaign, and to provide basic information for its conduct, a survey should be made to determine the degree of natural immunity to poliomyelitis and the amount of poliovirus and other enterovirus infection in the po ...196014403224
the pathogenesis of poliomyelitis. sites of multiplication of poliovirus in cynomolgus monkeys after alimentary infection. 196013843985
enteroviral ribonucleic acid. ii. biological, physical, and chemical studies.chromatographic behavior of whole type 1 poliovirus and phenol-extracted viral rna on diethylaminoethyl cellulose columns, as revealed by assay of plaque-forming capacity, indicated that infectious rna had surface properties markedly different from those of the intact virus. infectious rna of type 1 poliovirus and coxsackie b1 virus was relatively resistant to heat inactivation as compared to intact virus. kinetics of inactivation at elevated temperatures were multi-hit in character. the structu ...196013715282
enteroviral ribonucleic acid. i. recovery from virus and assimilation by cells.a relatively sensitive and adequately reproducible assay of infectious enteroviral rna was obtained by exposing calf serum-grown hela cells to rna suspended in 2 m magnesium sulfate solution. highly purified enteroviral preparations yielded rna exhibiting more than 0.1 per cent of the infectivity of whole original virus and infectivity regressed linearly with dilution. radioisotope experiments with p(32)-labeled rna and spectrophotometric studies demonstrated that gierer-schramm phenol extractio ...196013715281
[results of isolation and typing of poliomyelitis virus in hela cells in odessa]. 196013856435
[comparative study of various methods of titration of virus-neutralizing antibodies to poliomyelitis virus in tissue culture]. 196013850244
poliomyelitis virus in sewage in 1951. 196013850460
studies on antiviral immunity using tissue culture methods. ii. obtaining cells resistant to poliomyelitis virus. 196013854898
nutritional requirements for the production of poliovirus type ii. coxsackie b3, and vaccinia viruses by continuous animal cell cultures. 196013840107
research on the penetration of the attenuated polio virus strain type i (chat) into an unvaccinated population in wyszkow and the surrounding district. 196013827368
studies on the interaction between coxsackie and poliomyelitis viruses. iv. further experiments concerning the antagonism between coxsackie b1 virus and the lansing strain of poliomyelitis virus in young mice. 196013817429
[identification and antigenic typing of strains of poliomyelitis virus isolated in japan]. 196013820259
action of mucoproteins in vivo and in tissue culture on poliomyelitis virus. 196013820534
immunological strain specificity within type 1 poliovirus.the demonstration of immunological differences between poliovirus strains of any one type is a valuable procedure in epidemiological research as it may allow a virus strain to be identified as derived from or unrelated to a given possible source of infection. it is obviously of particular importance in connexion with live poliovirus vaccination campaigns. both kinetic tests and conventional neutralization and complement-fixation techniques have been used to this end, the former involving a more ...196013826481
assessment of correlation between certain in vitro poliovirus markers and monkey neurovirulence. 196013806668
production of viraemia in cortisone-treated monkeys with the attenuated brunenders strain of type-i poliovirus. 196013811059
[induction of mutations into the poliomyelitis virus by nitric acid]. 196013802074
[materials for the study of the sensitivity of lagurus lagurus pall to the poliomyelitis virus. i. the possibility of utilization of lagurus lagurus pall for a study of poliomyelitis viruses types ii and iv]. 196013737591
[inactivation and cellular specificity of the ribonucleic acid of the poliomyelitis virus]. 196013738071
[influence of ketoaldehyde derivatives of biphenyl on the dynamics of viral infections in monolayer cell cultures. ii. on the activity of xenalamine on the dynamics of infection by type i (brunhilde) poliomyelitis virus]. 196013695413
[influence of ketoaldehyde derivatives of biphenyl on the dynamics of viral infections in monolayer cell cultures. i. on the activity of xenaldial on the dynamics of infections by type i (brunhilde) poliomyelitis virus]. 196013695415
[influence of cortisone-type steroids on the cytopathogenic activity of viruses in tissue culture. iii. study of the effect of prednisolone on the cytopathogenic activity of type i (brunhilde) poliomeylitis virus]. 196013695418
[on the d-characteristic of polimyelitis virus]. 196013685453
biological properties of ribonucleic acid from virulent and attenuated poliovirus.concentrates of type i poliovirus were extracted with phenol by gierer and schramm's method. the infectivity of these extracts in tissue culture and mice appeared to be associated with viral rna. studies on neurovirulence based on the "t" marker and intraspinal pathogenicity in mice demonstrated that the viral progeny of rna isolated from virulent viruses had the "t(+)" character and were highly neurotropic for mice. conversely, rna extracted from attenuated virus gave rise to a progeny of "t" t ...196013704559
[epidemic infantile gastrointestinal syndrome caused by enteroviruses (echo-14, echo-9 and poliovirus of type 3)]. 196013709106
virus survival as a seasonal factor in influenza and polimyelitis. 196013713229
intracellular virus crystals in the central nervous system of mice following rodent polioencephalitis (mm strain) infection. 196013728271
[the effect of potassium permanganate on the poliomyelitis virus]. 196013734308
immunological and epidemiological effectiveness of live poliomyelitis vaccine in the ussr.in 1959 a total of 1 700 000 children up to 14-18 years old in the latvian, byelo-russian, moldavian and russian republics of the ussr were given live poliovirus vaccine prepared from attenuated sabin strains. the results show that the vaccine is highly effective and quite innocuous.the mean reduction in poliomyelitis incidence in the different republics varied from sixfold to tenfold, and among very young children (the age-group at greatest risk) was as high as 15-fold. in regions where live va ...196020604073
oral immunization against poliomyelitis.in the ten years since attenuated poliovirus was first administered to man for immunization against poliomyelitis, a vast body of experience has grown up and an attempt can now be made to assess the potentialities of oral poliovirus vaccine. the author of this paper seeks to answer two main questions: how safe are the strains now under study, and is oral vaccine effective in practice under the different conditions in which it may be used?from the information gained in large-scale trials involvin ...196020604072
the mammalian cell-virus relationship : v. susceptibility and resistance of cells in vitro to infection by coxsackie a9 virus.all primary cell cultures tested from numerous human and rhesus monkey tissues were susceptible to coxsackie a9 virus infection and were found to contain a specific a9 virus receptor. ability of these cells to adsorb actively virus correlated with the presence of this receptor. attachment of a9 virus to cell receptor was specifically enhanced by calcium and magnesium ions. human cells established in continuous culture from pretested susceptible cultures were found to have lost a9 virus susceptib ...196019867177
poliomyelitis immunization: mass use of oral vaccine in the united states might prevent definitive evaluation of either vaccine.i would summarize the foregoing discussion by asserting, first, that the superiority of the live oral attenuated poliovirus vaccines over the inactivated vaccines now in use remains to be demonstrated, except for convenience of administration. second, the proposal to introduce live oral poliovirus vaccine by means of country-wide mass immunization programs is irresponsible in the sense that such a procedure would eliminate the possibility of a definitive evaluation of either vaccine in this coun ...196117817390
aetiology of central nervous system infections during prevalence of poliovirus and coxsackie virus. 196120789119
vaccination with the chat strain of type 1 attenuated poliomyelitis virus in leopoldville, congo. 3. safety and efficacy during the first 21 months of study.between august 1958 and april 1960 nearly all children less than five years old in léopoldville were orally vaccinated with the chat, type 1, attenuated poliovirus strain of koprowski. in a previous report, the first nine months of the campaign were reviewed and tentative conclusions were drawn concerning the safety and efficacy of the vaccine. the present paper is a report on a further twelve-month period of observation. additional epidemiological data and virological studies, including the use ...196113736381
serum inhibition of cytopathic effect and virus release of type 3 poliovirus vaccine in primary monkey-kidney (mk) cultures. 196113731294
interaction of viruses in the intestinal tract of man. i. interference between wild and vaccine poliovirus strains. 196113724478
facilitation of infection of monkey cells with poliovirus "ribonucleic acid".the plaque titer of poliovirus "ribonucleic acid" on monkey kidney cells cultured in vitro is greatly increased by depleting these cells of calcium and treating the "ribonucleic acid" inoculum with a suspension of any one of several poorly water-soluble substances before inoculation. these undissolved substances apparently facilitate infection by serving as solid vehicles for the "ribonucleic acid."196113724573
a comparison of the growth properties of coxsackie virus strains a-9 and a-10 with poliovirus strain mahoney in cultures of primary human amnion cells. 196113725061
a phase contrast microprecipitin test with poliovirus antigens. ii. occurrence and development of microprecipitin antibody after natural inapparent infections, after inoculation of salk vaccine, after feeding of attenuated live poliovirus vaccine, and in disease caused by polioviruses and other enteroviruses. 196113725917
[serum avidity and virus aggregate in the poliomyelitis virus neutralization experiment]. 196113709432
metabolism of human amnion cell cultures infected with poliovirus. ii. utilization of glucose, pyruvate and ribose in virus infected cells. 196113688518
the effect of poliomyelitis virus type i (mahoney strain) on the phospholipid metabolism of the hela cell. 196113695554
the effect of environment on the replication of poliovirus in monkey kidney cells. 196113702674
[isolation of infectious antigens from poliomyelitis virus preparations]. 196113681646
antiviral effect of guanidine.it has been found that soluble guanidine salts show an antiviral effect on poliovirus and on some other enteroviruses. the activity was first detected in a cell culture screening system when the guanidine salt of hydroxyaminomethylene malononitrile inhibited the cytopathic effect of poliovirus in cell cultures. the studies were extended to various in vivo experiments in which the suggestive therapeutic activity of guanidine salts was again observed in monkeys infected with poliovirus. while thes ...196113741634
observations on the complement-fixing antibody response to poliovirus in patients with certain coxsackie and echo virus infections. 196113760855
[on the inhibitory activity, in vitro, of guanidine against the poliomyelitis virus. ii. lack of action of derivatives and analogues of guanidine]. 196113763014
[on the inhibitory activity, in vitro, of guanidine against the poliomyelitis virus. iii. lack of antagonism of certain molecules against the inhibition of cytopathogenic effect produced by guanidine in comparisons on poliomyelitis virus i]. 196113763015
[on the inhibitory activity, in vitro, of guanidine against the poliomyelitis virus. iv. influence of guanidine on viral multiplication]. 196113763016
[on the inhibitory activity, in vitro, of guanidine against the poliomyelitis virus. v. curative activity of guanidine in infections of cell cultures by poliomyelitis virus i]. 196113763017
[on the inhibitory activity, in vitro, of guanidine against the poliomyelitis virus. vi. recent research on the possible mechanism of antipoliomyelitic action of guanidine in vitro]. 196113763018
the effect of oxidation and reduction on the infectivity of poliomyelitis virus. 196113764266
reversibility of the reaction between polio-virus and neutralizing antibody of rabbit origin. 196113766052
studies on the acridine orange staining of two purified rna viruses: poliovirus and tobacco mosaic virus. 196113768614
presence of two interrferring enterovirral agents (echo virus type 9 and poliovirus type 2) in the human central nervous system. 196113780920
maintenace of intestinal tissue in vitro and the multiplication of poliovirus in excised segments of monkey intestinal tract. 196113783208
[the different reaction of attenuating and virulent strains of poliomyelitis virus to the action of chlorine and soil adsorbents]. 196113788114
proposed method and evaluation of the monkey neurovirulence test for attenuated poliovirus vaccine. 196113791071
[supplementary factors connected with manifestation of the d-factor in poliomyelitis virus (preliminary report)]. 196113859607
heterotypic antibody responses by rabbits inoculated with poliovirus. 196113865116
the influence of technique on the sensitivity of monkeys to intraspinally inoculated poliovirus. 196113868598
relationship between structure of benzimidazole derivatives and selective virus inhibitory activity. inhibition of poliovirus multiplication and cytopathic effects by 2-(alpha-hydroxybenzyl)-benzimidazole, and its 5-chloroderivative.the virus inhibitory activity and selectivity of certain benzimidazole, benzotriazole, and naphthimidazole derivatives were determined with influenza b and polio type 2 viruses. among the sixty-five compounds examined, several were highly active inhibitors of influenza b virus multiplication in the chorioallantoic membrane in vitro. the following compounds, listed in order of increasing inhibitory activity, were more than 100 times as active as benzimidazole: 5-(4'-toluenesulfonamido)-benzimidaz ...196113775109
the location and nature of enterovirus receptors in susceptible cells.it is shown that enterovirus receptors are found mainly in the microsomal fraction of disrupted primate cells. greater virus adsorption was exhibited by disrupted cells than by intact cells, indicating that enterovinis receptor may be present on intracellular membranes as well as on the surface of the cell. polio-virus receptor is an integral part of, or is firmly attached to, the insoluble lipoproteins of the cell. all attempts to solubilize receptor have either destroyed virus-adsorbing activi ...196113715283
[the elimination of viruses by fecal matter in an institution of children infected with type 1 poliomyelitis virus]. 196113868679
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