Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| therapeutic effects of roxithromycin and azithromycin in experimental murine brucellosis. | mice infected with brucella melitensis were treated with azithromycin or roxithromycin at a dose of 50 mg/kg/day i.p. alone and in combination with streptomycin 75 mg/kg/day for 14 days. streptomycin at this dose was previously documented to be ineffective against murine brucellosis. azithromycin- and azithromycin/streptomycin-treated animals demonstrated a significantly better cure rate than controls. therapy failure was observed in all mice treated with roxithromycin 50 mg/kg/day i.p. alone or ... | 1994 | 8082413 |
| in-vitro and in-vivo immunobiological properties of murine monoclonal anti-brucella antibodies. | features and protective activity of anti-brucella murine monoclonal antibodies (mabs) versus b. melitensis 16m were studied. all mabs tested were able to confer a good passive protection and showed correlative biological properties in immunological reactions. | 1994 | 7510505 |
| fusogenic activity of amino-terminal region of hiv type 1 nef protein. | we have studied two isoforms of nef, nef-27 and nef-25, which were produced in e. coli. nef-25 lacked the first 18 n-terminal residues of nef-27 and both were nonmyristylated. nef-27 fuses small unilamellar dipalmitoyl phosphatidylcholine vesicles (suvs), as indicated by enhanced light scattering of suvs and lipid mixing using concentration-dependent fluorescence dequenching. nef-27 also causes the appearance of a shifted isotropic peak in the 31p nmr spectra of these vesicles, suggesting that p ... | 1994 | 7848681 |
| differentiation of brucella abortus bv. 1, 2, and 4, brucella melitensis, brucella ovis, and brucella suis bv. 1 by pcr. | several pcr assays which identify the genus brucella but do not discriminate among species have been reported. we describe a pcr assay that comprises five oligonucleotide primers which can identify selected biovars of four species of brucella. individual biovars within a species are not differentiated. the assay can identify three biovars (1, 2, and 4) of b. abortus, all three biovars of b. melitensis, biovar 1 of b. suis, and all b. ovis biovars. these biovars include all of the brucella specie ... | 1994 | 7852552 |
| [detection of brucella with an automatic hemoculture system: bact/alert]. | the ability of in vitro and in vivo detection of brucella spp. with the bact/alert system was studied. | 1994 | 7865557 |
| immunotherapy in chronic brucellosis. effect of levamisole and interferon; mechanisms of action and clinical value. | thirty two anergic patients with chronic brucellosis treated with a) interferon-alpha 2b(group 1), b) levamisole (group 2) and c) conventional therapy(group 3) were studied. the effect of treatment on t lymphocyte blast formation in the presence of pha, specific cell mediated immunity against brucella antigens, titers of brucella antibodies and clinical symptoms were evaluated .t lymphocyte blast formation was shown to range in normal levels in all patients before treatment compared to 10 normal ... | 1994 | 7876467 |
| osteoarticular brucellosis resembling microcrystalline arthropathy. | 1994 | 7799378 | |
| national cross-sectional study of mastitis in dairy cattle in jordan. | between july 1991 and august 1992, 63 jordanian dairy farms selected by stratified random sample were visited to identify the major causes and prevalence of intramammary infections in dairy cows. of 773 cows examined 60% of all sampled quarters had > 283,000 cells/ml. the mean value of somatic cell count (scc) was positively associated with age in lactations and negatively with herd size. cows milked by bucket milking machines or in fully automatic parlours had a lower mean scc than those milked ... | 1994 | 7809990 |
| a case of brain abscess due to brucella melitensis. | 1994 | 7893897 | |
| brucella haemorrhagic pleural effusion. | brucella melitensis (bm) is a rare respiratory pathogen. the lungs are usually affected in the subacute and chronic course of the disease, when pleurisy, empyema, hilar adenopathy, pneumonia and lung abscess have been described. we present a patient with bm haemorrhagic pleural effusion, with low ph, low glucose and positive pleural fluid cultures. brucellosis should be considered in the differential diagnosis of patients with long-standing pleural effusions of unknown aetiology. | 1994 | 7925918 |
| vaccination with brucella abortus rough mutant rb51 protects balb/c mice against virulent strains of brucella abortus, brucella melitensis, and brucella ovis. | vaccination of balb/c mice with live brucella abortus rb51, a stable rough mutant, produced protection against challenge with virulent strains of brucella abortus, brucella melitensis, and brucella ovis. passive-transfer experiments indicated that vaccinated mice were protected against b. abortus 2308 through cell-mediated immunity, against b. ovis pa through humoral immunity, and against b. melitensis 16m through both forms of immunity. live bacteria were required for the induction of protectiv ... | 1994 | 7927779 |
| [the endotoxins of gram-negative bacteria: their structure and biological role]. | main attention in the paper is paid to the study of lipid a, a component possessing endotoxic activity. lipids a containing glucosamine disaccharide (representatives of enterobacteriaceae family), and variants of lipid a differing from the toxic one either in the structure of carbohydrate core or in the spectrum of fatty acids are considered. they are either phototrophic, nodulating (bradyrhyzobium species) or soil species (nitrobacter and thiobacillus) bacteria. lipid a from lipopolysaccharides ... | 1994 | 7952230 |
| brucella ribosomal protein l7/l12 is a major component in the antigenicity of brucellin inra for delayed-type hypersensitivity in brucella-sensitized guinea pigs. | a delayed-type hypersensitivity (dth) reaction in the course of brucellosis in humans and animals can be revealed by the brucellin inra (brucellergen) skin test. brucellergen is composed of more than 20 proteins of different molecular weights. a 12-kda protein eliciting dth in brucella melitensis rev1-sensitized guinea pigs was found to be a significant component for the allergenic properties of brucellergen. sequencing of the gene encoding this protein identified it as the l7/l12 ribosomal prot ... | 1994 | 7960115 |
| changing trends in the epidemiology of human brucellosis in california from 1973 to 1992: a shift toward foodborne transmission. | from 1973 through 1992, 426 cases of human brucellosis were reported in california, of which 98% were laboratory confirmed. brucella melitensis was identified in 185 cases (78.7% of the bacteriologically typed cases). hispanics accounted for 81% of the cases from 1983 to 1992 compared with 65% during the previous decade (p < .01). the population-adjusted average annual incidence was higher in hispanics, especially in children and teenagers, compared with non-hispanic whites and african americans ... | 1994 | 7963716 |
| [aortic valvular endocarditis caused by brucella melitensis with initial acute myocardial infarction manifestation]. | this is the case of a 31 year old man with brucella aortic valvular endocarditis which first manifested itself clinically as an acute myocardial infarction (ami). he subsequently developed an acute aortic regurgitation, which together with the ventricular disfunction secondary to the myocardial necrosis caused severe heart failure requiring aortic valve replacement and aortic coronary graft in a first operation. the patient's subsequent evolution was negative with congestive heart failure refrac ... | 1994 | 7973020 |
| evaluation of allergic and serological tests for diagnosing brucella melitensis infection in sheep. | a total of 291 unvaccinated sheep from brucella melitenesis-infected flocks were examined for delayed-type hypersensitivity (dth) responses with brucellergene commercial allergen and with cold saline extract and cytosol from rough b. melitensis 115, and their sera were tested in the rose bengal test (rbt), complement fixation test (cft), and enzyme-linked immunosorbent assay (elisa) with lipopolysaccharide. dth reactions were maximal after 72 h, with no intensity differences among allergens, ino ... | 1994 | 7989528 |
| detection of brucella melitensis by bactec nr660 blood culture system. | data on the performance of modern blood culture systems for the detection of brucella spp. are insufficient. to evaluate the performance of the bactec nr660 blood culture system for the detection of brucella melitensis within the routine 1-week blood culture protocol, a prospective 24-month study was conducted in an area endemic for b. melitensis in southern israel. blood samples obtained from patients with suspected brucellosis were monitored and blindly subcultured once per week for 4 weeks, a ... | 1994 | 7989539 |
| outbreak of brucella melitensis among microbiology laboratory workers. | we report on an outbreak of laboratory-acquired brucellosis involving four technicians working at a microbiology laboratory. all cases occurred in a period of 4 months. blood cultures and the rose bengal test were positive for brucella spp. in all cases. microagglutination was positive for brucella spp. at titers of between 1/40 and 1/160. all patients were cured after treatment. | 1994 | 7989566 |
| [an evaluation of the effectiveness of laboratory diagnostic methods for brucellosis]. | the diagnostic value of bacteriological and serological methods for the laboratory diagnosis of brucellosis was studied. in the analysis of milk and cheese specimens brucella cultures were isolated and differentiated as b.melitensis, biovar i, and b.abortus, biovar 4. in 25.6% of cases b.melitensis culture, biovar 1, was isolated from the blood of persons suspected for brucellosis. the isolation of b.melitensis culture from milk showed that this infective agent migrated from small animals to cat ... | 1994 | 7992538 |
| epidural abscess due to acute brucella melitensis infection. | 1994 | 8002693 | |
| efficacy of different rose bengal and complement fixation antigens for the diagnosis of brucella melitensis infection in sheep and goats. | fifteen different rose bengal antigens showed large differences with respect to ph, cell concentration and agglutination with the international standard anti-brucella abortus serum, demonstrating the lack of international standardisation. their sensitivity and specificity, compared with that of the complement fixation test, were evaluated for the diagnosis of b melitensis infection in culture-positive sheep, brucella-free ewes, and sheep and goats belonging to field flocks under different epidem ... | 1994 | 8036772 |
| efficacy of combinations of doxycycline and rifampicin in the therapy of experimental mouse brucellosis. | mice infected with brucella melitensis were treated with doxycycline and rifampicin in daily dosages of: 1.5, 3 and 6 mg/kg separately and in combination, for a period of 14 days. doxycycline alone in all dosages did not result in a cure rate superior to controls. a significant reduction in the viable count of b. melitensis recovered from the spleens of mice (which was the criterion for cure) was achieved with 6 and 3 mg/kg/day of doxycycline. rifampicin at a dose of 6 mg/kg/day gave significant ... | 1994 | 8040118 |
| evaluation of serological tests for diagnosis of brucella melitensis infection of goats. | five serological assays were evaluated for the diagnosis of brucellosis in goats: the rose bengal test (rbt), complement fixation test (cft), radial immunodiffusion (rid) with brucella and yersinia enterocolitica o:9 polysaccharides, counterimmunoelectrophoresis (ciep) with cytosol, and enzyme-linked immunosorbent assay (elisa) with polyclonal and protein g conjugates and smooth lipopolysaccharide (s-lps), native hapten polysaccharide (nh), or cytosol antigens. for optimal sensitivity, rbt had t ... | 1994 | 8051240 |
| fatal brucella melitensis endocarditis in a hemodialysis patient. | 1994 | 8072615 | |
| identification and nucleotide sequence of brucella melitensis l7/l12 ribosomal protein. | dna sequencing of the gene encoding a brucella melitensis 12-kda protein revealed that this protein was the ribosomal protein l7/l12. the b. melitensis l7/l12 dna sequence was identical to that of the corresponding b. abortus gene, showing the near identity of these two organisms. when comparing the sequence of this protein to that of other organisms some domains were highly conserved, especially the c-terminus, which contrasted with the lack of conservation of the sequences at the n-terminus. t ... | 1994 | 8076798 |
| activity of bay y 3118, a novel 4-quinolone, against brucella melitensis. | we have tested the in-vitro activities of bay y 3118, a new chlorofluoroquinolone, ciprofloxacin, sparfloxacin, streptomycin, tetracycline and rifampin against 59 strains of brucella melitensis. bay y 3118 (mic90 0.12 mg/l) was twice as active as sparfloxacin and tetracycline (mic90 0.25 mg/l). the activity of ciprofloxacin, rifampin and streptomycin (mic90 0.5, 2, and 8 mg/l, respectively) was, respectively, four-, sixteen-, and more than sixty-fold lower than that of bay y 3118. | 1994 | 8077983 |
| hematologic manifestations of brucellosis in children. | 1994 | 8078747 | |
| brucella melitensis cell envelope protein and lipopolysaccharide epitopes involved in humoral immune responses of naturally and experimentally infected sheep. | cell envelope fraction (cef) of brucella melitensis b115 was used to investigate antibody responses of b. melitensis naturally and strain h38 experimentally infected sheep by immunoblotting, indirect enzyme-linked immunosorbent assay (elisa) (i-elisa), and competitive elisa (c-elisa) with monoclonal antibodies (mabs). mabs used were directed to outer membrane proteins with molecular masses of 10, 16.5, 19, 25 to 27, 31 to 34, 36 to 38, and 89 kda; to the heat shock protein dnak, to o-polysacchar ... | 1994 | 7529242 |
| antibacterial activity of azithromycin against brucella melitensis. | in vitro antibacterial activity of the new macrolide azithromycin was tested against 116 strains of brucella melitensis, isolated from 115 patients in a major tertiary care referral center. eighty-seven percent of the strains were inhibited by 1.0 mg/l and all the 116 strains by 2.0 mg/l of azithromycin. comparison was made with tetracycline, gentamicin, trimethoprim-sulfamethoxazole, rifampicin and ciprofloxacin. all the isolates were susceptible to gentamicin, tetracycline, trimethoprim-sulfam ... | 1995 | 7555205 |
| surface exposure of outer membrane protein and lipopolysaccharide epitopes in brucella species studied by enzyme-linked immunosorbent assay and flow cytometry. | seven surface-exposed outer membrane proteins (omps) in brucella supp. have been previously described (a. cloeckaert, p. de wergifosse, g. dubray, and j. n. limet, infect. immun. 58:3980-3987, 1990). omps were shown to be more accessible to monoclonal antibodies (mabs) on rough (r) brucella melitensis and b. abortus strains than to mabs on their smooth (s) counterparts. in this work, we have extended this study to representatives of the main brucella species, using mabs specific for omps and s a ... | 1995 | 7558303 |
| comparative evaluation of microagglutination test and serum agglutination test as supplementary diagnostic methods for brucellosis. | the diagnosis of brucellosis in cattle and small ruminants requires the use of more than one serological test. the complement fixation test (cft), the rose bengal test (rbt), and the serum agglutination test (sat) are among the most useful tests for routine diagnosis. the microagglutination test (mat) was developed as a simpler and more efficient test than the sat. the relative efficacy of this test compared with that of the sat was evaluated by using brucella-free sheep and goats prior to and a ... | 1995 | 7559970 |
| thyroiditis due to brucella melitensis. | a case of thyroiditis due to brucella melitensis is reported. brucellosis anticipated by about two months the onset of the characteristic symptoms of acute thyroiditis. cultures of specimens obtained by fine needle aspiration biopsy and microbiological investigations allowed isolation and identification of the germ. this observation allowed the recognition that thyroid gland might harbored secondary localization of a prolonged brucellosis. a microbiological study (the protocol of which is propos ... | 1995 | 7560815 |
| growth phase-dependent variations in the outer membrane protein profile of brucella melitensis. | changes in brucella cell envelope protein profiles were investigated with batch cultures of b. melitensis strain 16m in a 2-litre fermenter. analysis of expression of outer membrane proteins (omp) (apparent molecular masses of 10, 16.5, 19, 25-27, 31-34, 36-38 and 89 kda) and heat-shock protein dnak (73 kda) was performed with monoclonal antibodies (mab) and immunoblotting techniques. synthesis of the 89-kda omp and the heat-shock protein dnak was invariant during b. melitensis growth. expressio ... | 1995 | 7569317 |
| study of the organization of the genomes of escherichia coli, brucella melitensis and agrobacterium tumefaciens by insertion of a unique restriction site. | tn5map, a tn5 derivative containing the 18 bp i-scei site, was delivered from a rp4-mobilizable, rk6-derived suicide vector to escherichia coli hb101, brucella melitensis and agrobacterium tumefaciens c58, which all lack natural i-scei sites in their genomes. digestion of the dna from tn5map-containing strains and analysis by pulsed-field gel electrophoresis (pfge) revealed that these derivatives contained a single transposon insertion. these digests also gave direct and independent proof for th ... | 1995 | 7582002 |
| evaluation of an enzyme-linked immunosorbent assay for the detection of sheep infected and vaccinated with brucella melitensis. | an indirect enzyme-linked immunosorbent assay (elisa), using the lipopolysaccharide of the cell wall as an antigen, was used to detect brucella melitensis antibodies in ovine serum. the test was carried out on 703 samples of field serums, which were also analyzed by the complement fixation (cf) test and the rose bengal (rb) test. the elisa results were more similar to those of the cf test (kappa = 0.89) than to the results of the rb test (kappa = 0.73). the elisa also had high sensitivity (94.7% ... | 1995 | 7619903 |
| characteristics of and risk factors for relapse of brucellosis in humans. | for 16 years we prospectively observed 530 adult patients with brucellosis to analyze the characteristics of and risk factors for relapse. clinical and laboratory findings from 86 relapsed patients were milder during the relapse episode when compared with those for the same patients during the initial disease. blood cultures were positive for brucella melitensis in 65% of cases during relapse and in approximately 80% of cases during the initial disease. risk factors that were identified as being ... | 1995 | 7620005 |
| comparison of the efficacy of brucella suis strain 2 and brucella melitensis rev. 1 live vaccines against a brucella melitensis experimental infection in pregnant ewes. | the comparative efficacy of brucella suis strain 2 (s2) and brucella melitensis strain rev. 1 (rev. 1) live vaccines in protecting sheep against b. melitensis infection was evaluated by clinical and bacteriological examination of ewes vaccinated conjunctivally with a dose of 1 x 10(9) c.f.u. when 4 months old and then challenged with 5 x 10(7) c.f.u. of the b. melitensis virulent strain 53h38 (h38) at the middle of the first or second pregnancy following vaccination. animals were considered to b ... | 1995 | 7625115 |
| susceptibilities of brucella melitensis isolates to clinafloxacin and four other new fluoroquinolones. | the susceptibilities of 120 clinical isolates of brucella melitensis and 3 reference strains of the same species to six fluoroquinolones (clinafloxacin, pd 117596, pd 131628, pd 138312, pd 140248, and ciprofloxacin) were examined by agar dilution mic methodology. clinafloxacin was the most active compound tested (mic at which 50% of strains tested were inhibited [mic50] and mic90 of 0.06 micrograms/ml). its level of activity was slightly higher than that of pd 117596 (mic50 and mic90 of 0.12 mic ... | 1995 | 7625815 |
| deletion of pure attenuates brucella melitensis 16m for growth in human monocyte-derived macrophages. | we constructed a defined purine-auxotrophic mutant of brucella melitensis 16m by chromosomal gene replacement. we electroporated b. melitensis 16m with suicide plasmids containing a kanamycin resistance cassette that replaced 226 bp at the carboxyl end of pure, the intergenic region, and 18 bases of the purk open reading frame. recombinant b. melitensis delta pure201 required exogenous purines for growth on minimal media. purine auxotrophy was complemented by electroporation of b. melitensis del ... | 1995 | 7642258 |
| protective effect of brucella outer membrane complex-bearing liposomes against experimental murine brucellosis. | liposomes of stable multilamellar type, which previously demonstrated great efficiency in antibiotic transport, were used in this study as transport vehicles of antigenic extracts of brucella melitensis (hs: complex of lipopolysaccharide/phospholipids/outer membrane proteins). the incorporation of hs into positively charged liposomes produced a protective effect against experimental murine brucellosis when they were administered 1 day before or 2 days after infection, as the number of colony-for ... | 1995 | 7649445 |
| in vitro efficacy of several antimicrobial combinations against brucella canis and brucella melitensis strains isolated from dogs. | in vitro efficacy of tetracyclines, aminoglycosides, fluoroquinolones, rifampin and macrolides and their combinations against four brucella canis and two b. melitensis strains isolated from dogs was evaluated by means of inhibition tests: minimal inhibitory concentration (mic) and fractional inhibitory concentrations (fic) and by bactericidal tests: minimal bactericidal concentrations (mbc) and kill-time experiments. in inhibition studies, tetracyclines showed the lowest mics of all compounds te ... | 1995 | 7653024 |
| cloning and sequence analysis of a newly identified brucella abortus gene and serological evaluation of the 17-kilodalton antigen that it encodes. | a thus far unknown gene encoding a brucella abortus protein has been isolated from a lambda gt11 expression library probed with sera from brucella-infected sheep. sequence analysis of the cloned gene revealed the presence of an open reading frame of 158 amino acids encoding a protein of 17.3 kda (calculated molecular mass). the recombinant b. abortus protein, expressed in escherichia coli, and the corresponding brucella melitensis protein migrated at the same apparent molecular masses as shown b ... | 1995 | 7664168 |
| cell response to a salt-extractable and sonicated brucella melitensis 16m antigen in human brucellosis. | we compared the immunological responses of leukocytes taken from healthy negative controls, laboratory workers immunized with the phenol-insoluble french vaccine against brucellosis, patients acutely ill with brucellosis, and patients chronically infected with brucella melitensis. a salt-extractable antigen (protein-rich but with traces of lipopolysaccharide) and a sonicated suspension from b. melitensis 16m were used as antigens for in vitro lymphocyte proliferation test. quantitation of t cell ... | 1995 | 7664186 |
| identification of sero-reactive brucella melitensis cytosoluble proteins which discriminate between antibodies elicited by infection and rev.1 vaccination in sheep. | distinction between brucella melitensis infected and vaccinated sheep is needed to fully achieve ovine brucellosis eradication in several countries. for this purpose, we probed immunoblots of cytosoluble protein extract (cpe) of the rough (r) b. melitensis strain b115 with sera of brucella-free, naturally infected, b. melitensis h38 experimentally infected and b. melitensis rev.1 vaccinated sheep to identify immunogenic brucella cytosoluble proteins which may lead to the development of more usef ... | 1995 | 7667905 |
| cloning and sequencing of the bacterioferritin gene of brucella melitensis 16m strain. | the 40 n-terminal amino acids of the 20 kda antigen a2 from brucella melitensis were sequenced and showed important similarities with 4 bacterioferrins. a monoclonal antibody raised against this antigen cross-reacted with escherichia coli bacterioferritin. hybridization of two sets of degenerate primers with b. melitensis hindiii-digested genomic dna identified a 3.8 kb fragment. this fragment was shown to contain a bacterioferritin gene (bfr) encoding a 161-amino acid protein. the sequence of t ... | 1995 | 7698330 |
| pseudogout masking brucellar arthritis. | 1995 | 7728410 | |
| bacterial infections: osteoarticular brucellosis. | osteoarticular brucellosis has been documented extensively from the middle east and spain in the last 5 years, but it has only been reported infrequently from the uk and usa. brucella melitensis from goat and sheep is the most frequently isolated organism. peripheral articular pain, particularly of the large joints, is the commonest osteoarticular manifestation, while effusions that seldom yield organisms on culture, also occur frequently. sacroiliitis which most frequently is unilateral, often ... | 1995 | 7728879 |
| brucella melitensis: an unexpected isolate from cerebrospinal fluid. | a specimen of cerebrospinal fluid was initially handled with 'category 3' precautions because the patient came from somalia, where tuberculosis and hiv infection are endemic. an isolate from the specimen, initially thought to be a neisseria species, was subsequently handled on the open bench. it was later identified as brucella melitensis. laboratory procedures should allow for the possibility of brucella in such specimens until a positive identification has been made. | 1995 | 7735352 |
| specific bovine brucellosis diagnosis based on in vitro antigen-specific gamma interferon production. | in order to improve the specificity of the diagnosis of bovine brucellosis, we developed a test which can be regarded as an in vitro correlate of the delayed-type hypersensitivity test (dth). a mixture of cytoplasmic proteins from brucella melitensis b115 was used as a specific antigenic stimulus in bovine whole blood culture. supernatants harvested at 18 to 24 h after the in vitro antigenic stimulus were assayed for their gamma interferon (ifn-gamma) content by using a commercial sandwich enzym ... | 1995 | 7751381 |
| in vivo and in vitro stability of the broad-host-range cloning vector pbbr1mcs in six brucella species. | previous studies have shown that the broad-host-range plasmid pbbr1mcs can be used for genetic complementation in brucella abortus. to extend these observations, the in vivo and in vitro stability of pbbr1mcs was evaluated in the six currently recognized species of the genus brucella. pbbr1mcs was readily introduced into all of the strains tested by electroporation and was stably maintained in broth cultures without antibiotic selection during five serial passages over a 10-day period. furthermo ... | 1995 | 7753908 |
| [brucellar bursitis]. | 1995 | 7754159 | |
| enhanced cellular response in mice treated with a brucella antigen-liposome mixture. | the capacity of liposomes constituted by dycetyl-phosphate (0.009 mm), cholesterol (0.017 mm), lecithin (0.003 mm), and myristic (0.1 mm), stearic (0.1 mm), or oleic acid (0.1 mm) to modify the lymphocyte response to brucella melitensis antigens in mice was studied. mice treated with antigens mixed with liposomes containing myristic, stearic or oleic acid had higher antibody titres than mice given antigen suspended in a saline solution. liposomes alone, without brucella antigens, resulted in inc ... | 1995 | 7773240 |
| serological responses to brucellosis in hiv-seropositive patients. | serial sera from 2 patients infected with the human immunodeficiency virus (hiv) type 1 attending a clinic in nairobi, kenya, and with blood cultures yielding brucella melitensis, were tested by enzyme-linked immunosorbent assay for their serological response (brucella-specific immunoglobulin (ig) m and igg) to brucella infection. antibody responses were comparable to those of immunocompetent individuals, one patient showing serology typical of acute brucellosis, the other of chronic brucellosis ... | 1995 | 7778159 |
| secondary amenorrhoea due to persistent corpus luteum in brucellosis. | 1995 | 7823703 | |
| microangiopathic hemolytic anemia and severe thrombocytopenia in brucella infection. | a case of brucella septicemia presenting at the onset as a severe microangiopathic hemolytic anemia with coexisting dramatic hemorrhagic syndrome (severe epistaxis, gross hematuria, and skin purpura) is reported. a hemogram showed severe thrombocytopenia, anemia, and leukopenia. bone marrow morphology showed the typical features associated with brucella infection: numerous histiocytes with signs of activation, multiple granulomata, giant cells, and hemophagocytosis. after appropriate antimicrobi ... | 1995 | 7827209 |
| restriction site polymorphism of the genes encoding the major 25 kda and 36 kda outer-membrane proteins of brucella. | seventy-seven brucella reference and field strains from different geographic origins and hosts representing the six recognized species and their different biovars were analysed for diversity of their genes encoding the major 25 and 36 kda outer-membrane proteins (omps) by pcr-rflp. the 25 kda omp is encoded by a single gene (omp25) whereas two closely related genes (omp2a and omp2b) encode and potentially express the 36 kda omp. analysis of pcr products of the omp25 gene digested with nine restr ... | 1995 | 7496522 |
| immunogenic properties of brucella melitensis cell-wall fractions in balb/c mice. | the immunogenicity of several brucella melitensis cell-wall (cw) fractions was tested in balb/c mice. these cw fractions were smooth lipopolysaccharide (s-lps) fraction from smooth (s) b. melitensis strain 16m, sodium dodecyl sulphate-insoluble (sds-i) cw fraction from b. melitensis strain 16m (s) undigested or digested with pepsin, and sds-i cw fraction from rough (r) b. melitensis strain h38. the b. melitensis sds-i cw fraction contained two major outer-membrane proteins (omps) of 25-27 kda an ... | 1995 | 7884802 |
| acute pancreatitis associated with brucellosis. | acute pancreatitis developed in a patient with acute brucellosis due to brucella melitensis. to the best of our knowledge this is only the second reported case of acute pancreatitis associated with brucella infection. | 1995 | 8580415 |
| single-step pcr for detection of brucella spp. from blood and milk of infected animals. | a versatile method for the extraction of brucella dna and pcr are presented as reliable tools for the detection of brucella spp. from body fluids of infected animals. two oligonucleotides homologous to regions of the gene encoding for an outer membrane protein (omp-2) were designed to detect the pathogen from milk and/or blood of infected goats, bovines, and human patients. the sensitivity of our test and its ability to detect the pathogen in samples from the field reveal a promising advance in ... | 1995 | 8586678 |
| [the epidemiological characteristics of brucellosis in the region of the northern caucasus]. | at present, the northern caucus region is the most unfavourable brucellosis area of the russian federation where 43.5% of the total russian incidence of this infection was recorded in 1993. over the past years (1988-1993) there was a highly unsteady-state improvement of the epidemiological situation, however, the rates of human morbidity with this infection greatly lag behind those in russia. the active foci of small cattle brucellosis as the leading source of human contamination with brucellosi ... | 1995 | 8587517 |
| antibiotic treatment induces an increase of the specific antibody levels in brucella melitensis infected mice. | the effects of doxycycline (dox) and streptomycin (sm) treatment of brucella melitensis infected mice on humoral immune response were examined. in female balb/c mice, dox was administered at a dose of 50 mg/kg/12 h, for 21 or 45 consecutive days, alone or combined with sm (10 mg/kg/12 h) for 14 days. all treatments induced a significant increase in specific igg levels (elisa and ciep) against lps and cytosolic antigens of brucella during treatment. this was not related with therapeutic failure o ... | 1995 | 8589668 |
| comparative activity of azithromycin and doxycycline against brucella spp. infection in mice. | the activities of a short therapeutic regimen with azithromycin and the classic treatment doxycycline with streptomycin were compared and evaluated in mice infected with brucella melitensis. in a chronic model, starting therapy 31 days after challenge, azithromycin (10 days, 50 mg/kg/day) significantly reduced the infection (2.9 logs, day 48 post-infection). the effectiveness of doxycycline (21 days, 50 mg/kg/12 hourly) was greater than azithromycin (4.1 logs of reduction, day 48 post-infection) ... | 1995 | 8591939 |
| control of brucella melitensis infection in a large camel herd in saudi arabia using antibiotherapy and vaccination with rev. 1 vaccine. | the authors describe an attempt to control brucella melitensis infection in a large camel herd in saudi arabia. sera from the entire herd (2,536) were examined by the rose bengal and standard united states of america buffered plate agglutination tests. the overall brucella seroprevalence was 8%. milk samples from the 120 seropositive milking camels were cultured on brucella-selective media. b. melitensis biovars 1, 2 and 3 were isolated from 41 camels (34%). seropositive camels (202) were treate ... | 1995 | 8593404 |
| clinical and therapeutic features of childhood neurobrucellosis. | brucellosis is a multisystem disease with diverse clinical presentations, and involvement of the nervous system is considered to be rare in childhood. five children with meningitis (n=2), meningoencephalitis (n=1), meningomyelitis (n=1), or cerebellar ataxia (n=1) are described, all of whom had a history of exposure to a possible source of brucellosis. examination of cerebrospinal fluid (csf) revealed lymphocytic pleocytosis in 4 patients, high protein concentration in 5 and low glucose concentr ... | 1995 | 8658066 |
| a case of brucella melitensis endocarditis successfully treated by a combination of surgical resection and antibiotics. | endocarditis is a rare complication of brucellosis, often fatal, and the treatment is controversial. we present a case of brucella melitensis endocarditis successfully treated by a combination of surgical resection and antibiotics. | 1995 | 8685649 |
| [isolation of brucella melitensis biovar 2 from the human milk]. | 1995 | 8814668 | |
| brucellosis associated with unpasteurized milk products abroad. | 1995 | 8519642 | |
| immunological response in mice after long-term stimulation with cell wall antigens from brucella melitensis. | the continuous stimulation of the immune system using cell wall antigens from brucella melitensis was found to cause both quantitative and qualitative changes in circulating lymphocyte populations in mice. animals were inoculated in the hind legs with antigens on alternate days for varying lengths of time. during a two-month period, we saw a higher number of circulating lymphocytes, with an increase in the number of cd4+ cells (l3t4+) and b lymphocytes (i-ad). after two months, a drop in the ove ... | 1995 | 8525067 |
| brucellar bacteremia in pregnancy. | 1995 | 8527594 | |
| brucellosis presenting with cough. | 1995 | 8532438 | |
| [azithromycin, pharmacodynamic evaluation in animal models]. | several experimental models have been used in order to evaluate the in vivo efficacy of azithromycin against numerous human pathogenic bacteria and parasites, including comparison between azithromycin and other antibiotics belonging or not to the macrolide family. using the experimental models, three major objectives can be distinguished: the comparative studies of the efficacy dose 50 (ed50) of azithromycin compared to other orally given antibiotics, the azithromycin efficacy in animal infected ... | 1995 | 8539074 |
| doxycycline-rifampin versus doxycycline-streptomycin in treatment of human brucellosis due to brucella melitensis. the gecmei group. grupo de estudio de castilla-la mancha de enfermedades infecciosas. | brucellosis is a common zoonosis in many parts of the world; the best regimen for the treatment of brucellosis has not been clearly determined. we have carried out a multicenter, open, controlled trial in five general hospitals in spain to compare the efficacy and safety of doxycycline and rifampin (dr) versus doxycycline and streptomycin (ds) for the treatment of human brucellosis. the study included 194 ambulatory or hospitalized patients with acute brucellosis, without endocarditis or neurobr ... | 1995 | 8540716 |
| a brucella melitensis high temperature requirement a (htra) deletion mutant demonstrates a stress response defective phenotype in vitro and transient attenuation in the balb/c mouse model. | bacterial stress response proteins of the high temperature requirement a (htra) family are serine proteases which appear to play an important role in scavenging oxidatively damaged proteins from the cell before they reach toxic levels. an isogenic htra deletion mutant, designated rwp5, was constructed from virulent brucella melitensis 16m via gene replacement to determine whether the b. melitensis htra protein functions as a stress response protein, and to evaluate the contribution of this prote ... | 1995 | 8778563 |
| significance of environmental conditions (ph and serum) on the in vitro potency of azithromycin against brucella melitensis. | 1995 | 8904096 | |
| antibrucella activity of quinolone sparfloxacin. | background: sparfloxacin is a new fluoroquinolone with excellent in vitro activity against a variety of gram-positive and gram-negative bacteria. methods: in vitro antibacterial activity of this new drug was tested against 139 strains of brucella melitensis. these strains were isolated from 138 patients at a major tertiary care referral center in riyadh, saudi arabia. results: seven percent of the strains were inhibited by 0.06 mg per l and 99% by 0.12 mg per l of sparfloxacin. of the five other ... | 1995 | 9815397 |
| human to human transmission of brucella melitensis. | human brucellosis is acquired mainly through contact with infected animal tissues, ingestion of unpasteurized dairy products or infected aerosols. person to person transmission is still considered uncertain. the case of a woman diagnosed with proven brucellosis after her husband suffered a relapse of bacteremia with brucella melitensis biotype 3, which was originally acquired abroad by eating goat cheese, is described. it was postulated that person to person spread of brucellosis is a likely mod ... | 1995 | 22514390 |
| [historical brief on the fever of malta]. | malta fever (brucellosis) is an infectious disease of cattle (chiefly goats) which can infect man. although the disease is not limited to malta, occurring also in other mediterranean countries as well as in asia and america, it was originally described in 1863 in malta where the responsible bacteria (brucella melitensis) was discovered in 1887 by d. bruce. ten years later wright & smith (1897) showed that the agglutination test originally established for typhoidic patients could be used for the ... | 1996 | 11624839 |
| brucella infection of the thyroid gland. | three cases of brucella infection of the thyroid gland are reported. all three were female, and two were from rural areas in tehran province. all presented with fever, chills, and painful swelling in the neck, had positive wright and 2-mercaptoethanol test; and had normal thyroid function. cultures of fna materials grew brucella in all cases. they were cured following antibiotic treatment. | 1996 | 8936672 |
| outer membrane protein of neisseria meningitidis as a mucosal adjuvant for lipopolysaccharide of brucella melitensis in mouse and guinea pig intranasal immunization models. | a mucosal vaccine against brucellosis consisting of the lipopolysaccharide (lps) of brucella melitensis complexed with the outer membrane protein (gbomp) of group b neisseria meningitidis was tested in small-animal models of intranasal immunization. mice given two doses of the vaccine developed high levels of immunoglobulin g (igg) and iga antibodies specific for b. melitensis lps in lung lavages and specific igg and iga antibody-secreting cells in the lungs and spleen. similarly, in guinea pigs ... | 1996 | 8945575 |
| breast abscess caused by brucella melitensis. | cutaneous and soft tissue lesions are uncommon manifestations of brucellosis. though breast involvement in animal brucellosis is not uncommon, involvement of the breast in human brucellosis is extremely rare. we report a case of breast abscess in a 39-year-old female caused by brucella melitensis. treatment with combination of trimethoprim/sulphamethoxazole (tmp/ smx; cotrimoxazole) and doxycycline for 3 months resulted in clinical cure. | 1996 | 8945713 |
| recombinant brucella abortus proteins that induce proliferation and gamma-interferon secretion by cd4+ t cells from brucella-vaccinated mice and delayed-type hypersensitivity in sensitized guinea pigs. | optimal protective immunity to brucella abortus infection is dependent on a coordinate interaction between different t-cell subsets which leads to an antigen-specific t-lymphocyte-mediated activation of macrophages, the main cellular reservoir for the bacterium. as an initial step in the identification of bacterial proteins that mediate cellular immunity, we have subcloned the b. abortus ssb, uvra, groes, and groel genes into the prokaryotic expression vector pmal-c2 using pcr. escherichia coli ... | 1996 | 8964089 |
| brucella melitensis biotype 1 outbreak in goats in northern kwazulu-natal. | 1996 | 8786610 | |
| effect of antibiotics contained in two brucella selective media on growth of brucella abortus, b. melitensis, and b. ovis. | the mic and the highest concentration enabling bacterial growth (ceg) of the antibiotics contained in two selective media were determined for brucella abortus, b. melitensis, and b. ovis. the nalidixic acid and bacitracin contained in farrell's selective medium were responsible for the inhibitory effects observed. | 1996 | 8789029 |
| polymorphism at the dnak locus of brucella species and identification of a brucella melitensis species-specific marker. | the dnak gene and surrounding sequences from reference strains of the six brucella species were amplified by the polymerase chain reaction (pcr) with primers chosen according to the published sequence of the b. ovis dnak gene and studied for polymorphism with nine restriction endonucleases. the restriction patterns were identical for all species with all restriction endonucleases tested except for b. melitensis strain 16m that showed a different pattern with ecorv, consistent with the presence o ... | 1996 | 8810947 |
| production and characterisation of monoclonal antibodies to brucella melitensis cytosoluble proteins that are able to differentiate antibody responses of infected sheep from rev. 1 vaccinated sheep. | monoclonal antibodies (mabs) were produced to brucella melitensis cytosoluble proteins (cp) with apparent molecular masses of 12, 24 and 28 kda (cp12, cp24 and cp28) which were previously shown by immunoblotting to differentiate antibody responses of infected sheep from those of b. melitensis strain rev. 1 vaccinated sheep. these mabs were derived from mice infected with virulent smooth (s) b. melitensis strain h38. most mabs obtained were directed to cp28, which indicated (as was shown in infec ... | 1996 | 8810948 |
| thrombosis of the abdominal aorta secondary to brucella spondylitis. | spondylitis is one of the most frequent osteoarticular complications of brucella infection in adults. occasionally it gives rise to soft tissue paravertebral or epidural masses, which can compress surrounding structures. a patient with thrombosis of the abdominal aortic artery secondary to brucella lumbar spondylitis is presented here, a complication that has not been previously reported. | 1996 | 8811368 |
| [epidemic outbreak of 81 cases of brucellosis following the consumption of fresh cheese without pasteurization]. | in spite of the great effort that has been made in recent years in castilla-la mancha to control brucelosis, a lack of awareness on the part of producers and consumers leads to major epidemic outbreak, such as the one described below. | 1996 | 9005033 |
| competitive enzyme-linked immunosorbent assay using monoclonal antibodies to the brucella melitensis bp26 protein to evaluate antibody responses in infected and b. melitensis rev.1 vaccinated sheep. | competitive enzyme-linked immunosorbent assay (c-elisa) was performed using 15 monoclonal antibodies (mabs), specific for brucella bp26 (previously also called cp28), a periplasmic protein antigen, to investigate antibody responses in naturally and b. melitensis h38 experimentally infected and b. melitensis rev.1 vaccinated sheep. the antigen preparation consisted of cytosoluble protein extract (cpe) of b. melitensis b115. by combining the c-elisa results of several mabs, a high percentage of na ... | 1996 | 9008343 |
| detection of brucella melitensis by bactec nr 730 and bactec 9120 systems. | in many parts of the world, brucellosis has significantly decreased, but it is still a problem in some regions of turkey. in our laboratory we have isolated 58 brucella spp. through bactec nr 730 and 30 brucella spp. through bactec 9120 systems. 31 of the 58 isolates were detected by bactec nr 730 system in 72 hours. the majority of the growth values were between 45-65. bactec 9120 system detected all the isolates in 84 hours. | 1996 | 8982628 |
| [infectious-allergic ophthalmic brucellosis (the validation of a new nosological form)]. | the necessity of distinguishing a new nosological entity: infectious allergic ophthalmic brucellosis-is validated. based on her own clinical and experimental findings and published data, the author describes the etiology, pathogenesis, clinical picture, diagnosis, and treatment of this disease. | 1996 | 9045486 |
| molecular characterization, occurrence, and immunogenicity in infected sheep and cattle of two minor outer membrane proteins of brucella abortus. | screening of a brucella abortus genomic library with two sets of monoclonal antibodies allowed the isolation of the genes corresponding to two minor outer membrane proteins (omp10 and omp19) found in this bacterial species. sequence analysis of the omp10 gene revealed an open reading frame capable of encoding a protein of 126 amino acids. the nucleotide sequence of the insert producing the omp19 protein contains two overlapping open reading frames, the largest of which (177 codons) was shown to ... | 1996 | 8557326 |
| characterization of brucella abortus and brucella melitensis native haptens as outer membrane o-type polysaccharides independent from the smooth lipopolysaccharide. | brucella native haptens (nhs) extracted with hot water from smooth (s)-type b. abortus and b. melitensis were purified to high levels of serological activity and compared with the polysaccharide obtained by acid hydrolysis (ps) of the s lipopolysaccharide (s-lps). by 13c nuclear magnetic resonance analysis, nhs showed the spectrum of a homopolymer of alpha-1,2- or alpha-1,2- plus alpha-1,3-linked 4-formamido-4,6-dideoxy-d-mannose (n-formylperosamine) previously reported for the lps o chain. howe ... | 1996 | 8576040 |
| infective endocarditis and occult splenic abscess caused by brucella melitensis infection: a case report and review of the literature. | this report describes a patient with prosthetic aortic valve endocarditis caused by brucella melitensis infection with an initial relapse after apparent recovery due to associated occult splenic abscess. in spite of a prolonged period of recovery (6 months) achieved with splenectomy and antibrucella antimicrobial therapy, a second relapse occurred and cure was achieved after replacement of the infected prosthetic valve and prolonged courses of antibrucella antimicrobial therapy. there is controv ... | 1996 | 8817011 |
| infected abdominal aortic aneurysm caused by spinal brucellar infection. | 1996 | 8819420 | |
| childhood brucellosis in israel. | brucellosis has become a major medical problem in israel particularly in the muslim arab population. | 1996 | 8823856 |
| first report of a strain of brucella melitensis that was widely sensitive to brucellaphages isolated in the united arab emirates. | 1996 | 8825005 | |
| inadequate azithromycin activity against brucella melitensis in mice with acute or chronic infections. | the activities of therapeutic regimens with azithromycin (azi) and doxycycline combined with streptomycin (dox-sm) were compared in brucella melitensis infected mice. in a chronic model, azi given over 10, 14 or 21 consecutive days (50 mg/kg/24 h) significantly reduced the infection (1.3-1.6 logs, day 48 post-infection). however, the effectiveness of dox (21 days, 50 mg/kg/12 h) was higher than azi (3.4 logs of reduction, day 48 post-infection). besides, when dox was administered for 45 days, it ... | 1996 | 8835110 |
| chronic subdural empyema: a new presentation of neurobrucellosis. | 1996 | 8842288 | |
| serological follow-up of human brucellosis by measuring igg antibodies to lipopolysaccharide and cytoplasmic proteins of brucella species. | a serological 2-year follow-up of 35 patients who had brucellosis with different clinical outcomes was performed. patients were followed up by standard tube agglutination (sta) and 2-mercaptoethanol sta (2me-sta) tests and by two enzyme-linked immunosorbent assays (elisas) to independently measure values of serum igg to either lipopolysaccharide (lps) or cytoplasmic proteins of brucella. whereas sta and 2me-sta tests revealed characteristic antibody profiles for patients who recovered (group i), ... | 1996 | 8852961 |
| hepatosplenic abscesses due to brucella melitensis: report of a case involving a child and review of the literature. | suppurative disease of the liver and/or spleen is a rare and serious complication of human brucellosis. in the english-language literature, only nine cases have been reported, all involving adults with chronic infection. we report the case of a young child in whom abscesses of the liver and spleen developed during acute brucellosis. brucella melitensis was cultured from an aspirate of the liver and from the bone marrow. after percutaneous drainage of the liver abscess, the patient responded to a ... | 1996 | 8852967 |