Publications

an investigation into the use of sds-page of cell surface extracts and proteolytic activity to differentiate prevotella nigrescens and prevotella intermedia.	by comparison of the cell surface proteins derived from the outer membrane and fibrils from 14 prevotella intermedia and 19 prevotella nigrescens strains using sds and analysed by sds-page, it was possible to distinguish the two species. a polypeptide of approx. 21 kda distinguished p. intermedia strains, whereas two polypeptides of approx. 18 and 22 kda could be used to identify p. nigrescens strains. four other human oral black pigmented bacterial species (porphyromonas gingivalis, prevotella ...	1996	8869494
[cerebral abscess in childhood: a report of 5 cases].		1996	8967649
plaque ph and microflora of dental plaque on sound and carious root surfaces.	given the hypothesis that root caries is the result of acid formation by acidogenic micro-organisms, the present study was performed to relate sucrose-induced ph response of dental plaque on root surfaces to the microbial composition of the overlying plaque. seventeen caries-active elderly chinese with poor oral hygiene and with both sound and carious root surfaces were examined. plaque ph was measured before and up to one hour after a controlled sucrose mouthrinse. plaque samples for microbiolo ...	1996	9003238
[severe orbital cellulitis: therapeutic results in 9 patients and review of the literature].	orbital cellulitis can produce severe neuromeningeal infections. modern antimicrobial agents such as imipenem can be a valid therapeutical choice.	1996	9044641
associations of endodontic symptoms and signs with particular combinations of specific bacteria.	significant associations have been reported between (a) specific bacterial species isolated from root canals and (b) between individual bacterial species and endodontic symptoms and signs. the prime objective of this study was to determine whether particular combinations of specific bacteria are associated with individual endodontic symptoms and signs. seventy root canals were investigated microbiologically taking care to maintain the viability of obligate anaerobes, which accounted for 64% of t ...	1996	9206427
[antimicrobial properties of flos lonicerae against oral pathogens].	the antimicrobial effect of flos lonicerae on oral pathogens was studied. the results showed that 73.9% of the tested pathogens were inhibited at a concentration below 6.25mg/ml. streptococci mutants, actinomyces viscosus and bacteroides melaninogenicus were comparatively more sensitive to flos lonicerae.	1996	9208561
incidence of anaerobes in ventilator-associated pneumonia with use of a protected specimen brush.	the role of anaerobic bacteria in ventilator-associated pneumonia (vap) has been little investigated. in this study we analyzed the incidence of anaerobes in patients with a first episode of bacteriologically documented vap (> 10(3)cfu/ml), using protected specimen brushes (psb). we particularly took care to preserve anaerobic conditions during transport and the microbiological procedure. two groups were considered: group a with anaerobic bacteria recovered from psb, with or without anaerobes, a ...	1996	8616556
enhancement of growth of group a beta-hemolytic streptococci in mixed infections with aerobic and anaerobic bacteria.	objective: to evaluate the potential for mutual enhancement of growth of group a beta-hemolytic streptococci (gabhs), and 11 commonly associated aerobic and anaerobic microorganisms frequently isolated in tonsillar infections. methods: enhancement was assessed by measuring the relative increase in colony-forming units (cfu) of gabhs and each of the 11 microorganisms inducing a subcutaneous abscess in mice. results: of the 11 combinations of gabhs and aerobe or anaerobe, gabhs was enhanced in 10 ...	1996	11866754
[study on local huangjiabang delivery device].	this article reports the pharmacological functions of local huangjiabang delivery device which mainly includes medicinal herbs. the experimental study showed that huangjiabang could inhibit the predominant cultivable organisms in periodontal disease significantly, and could also inhibit inflammatory swelling caused by xylene. jiaochacaijiao, egg serum and filter-paper-granuloma significantly. the preliminary clinical observation showed that gi, pli, sbi and pd were reduced remarkably in huangjia ...	1996	9387558
[the effect of tinidazole in the treatment of adult periodontitis].	fifty-two adult periodontitis patients were treated by the tablets of tinidazole (tnz), and 23 patients treated by metronidazole (mnz) served as control group. the effective rate of tnz in adult periodontitis patients was 73.1%, which was significantly higher than that of the control group (43.5%). antimicrobial studies showed that the capability of tnz to kill the periodontal dominant anaerobic bacteria, especially b. gingivalis and b. melaninogenicus, was better than that of mnz. the minimal i ...	1996	9592262
[bacterial flora in chronic inner ear infections in adults].	the aim of the study was to analyse microbiologically middle ear exudate obtained from 56 patients, aged 17 to 83 years, treated for chronic otitis media. aerobic bacteria only were found 49 patients (87,5%). mixed aerobic and anaerobic isolates were recovered from 7 patients (12,5%). the most common bacteria isolated from the middle ear exudate, in descending order frequency, were staphylococcus aureus (45%), pseudomonas aeruginosa (34%), proteus mirabilis (16%) and prevotella melaninogenica (9 ...	1997	9411077
evaluation of antimicrobial efficacy of various root canal filling materials for primary teeth.	four materials viz. zinc oxide-eugenol, iodoform paste, kri paste, maisto's paste and vitapex (calcium hydroxide + iodoform) were tested for their antibacterial effect against the aerobic and anerobic bacteria, viz. staphylococcus aureus, streptococcus viridans, streptococcus faecalis, bacteroides melaninogenicus and mixed bacterial culture; obtained from infected non-vital deciduous anterior teeth. the antimicrobial sensitivity was checked on bhi-agar plates using well method. the results showe ...	1997	10635127
[a study of anaerobic infection in maxillofacial region].	to analyse the anaerobic infection of maxillofacial surgery and estimate the efficacy of antianaerobic therapy, 45 patients were divided into two groups, tinidazole group and metronidazole group. bacterial culture was positive before treating in all cases. there were bacillus melaniogenicus, veillonella, peptococcus and peptostreptococus, etc. there was excellent efficacy in the treatment of maxillofacial anaerobic infection by tinidazole intravenously. after treatment, the result of bacterial e ...	1997	9868121
inhibition of prevotella and capnocytophaga immunoglobulin a1 proteases by human serum.	oral prevotella and capnocytophaga species, regularly isolated from periodontal pockets and associated with extraoral infections, secret specific immunoglobulin a1 (iga1) proteases cleaving human iga1 in the hinge region into intact fab and fc fragments. to investigate whether these enzymes are subject to inhibition in vivo in humans, we tested 34 sera from periodontally diseased and healthy individuals in an enzyme-linked immunosorbent assay for the presence and titers of inhibition of seven pr ...	1997	9220164
time-kill study of the activity of trovafloxacin compared with ciprofloxacin, sparfloxacin, metronidazole, cefoxitin, piperacillin and piperacillin/tazobactam against six anaerobes.	a time-kill method was developed to examine the killing kinetics of trovafloxacin, ciprofloxacin, sparfloxacin, metronidazole, cefoxitin, piperacillin and piperacillin/tazobactam against one strain each of bacteroides fragilis, bacteroides thetaiotaomicron, prevotella melaninogenica, fusobacterium mortiferum, peptostreptococcus magnus and clostridium perfringens. solutions and suspensions were prepared inside an anaerobic glove box, using syringes and prereduced broth. bottles were then incubate ...	1997	9222066
beta-lactamase production by oral pigmented prevotella species isolated from young children.	the frequency of beta-lactamase production by oral pigmented prevotella species isolated from 23 healthy young children and the minimal inhibitory concentrations (mics) for 186 available beta-lactamase-positive isolates were examined by using the chromogenic cephalosporin disk test (ab biodisk, solna, sweden) and the etest (ab biodisk) and/or the agar dilution method of the national committee for clinical laboratory standards (villanova, pa, usa), respectively. beta-lactamase-positive prevotella ...	1997	9310704
fermentation product analysis in the identification of black-pigmented bacteria from periodontitis.	a quantitative procedure is described for the analysis of fermentation products of eight representative black-pigmented gram-negative anaerobic bacterial strains (porphyromonas gingivalis 381, porphyromonas gingivalis atcc 33277, prevotella intermedia atcc 25611, prevotella nigrescens atcc 33563, prevotella melaninogenica atcc 25845, prevotella denticola atcc 33185, and prevotella loescheii atcc 15930) from oral sites in humans, using gas-liquid chromatography. this procedure for the identificat ...	1997	9345789
microbial findings of deep peri-implant bone defects.	the objective of this study was to examine inflammatory tissue in deep peri-implant bone pockets (> 5 mm) for anaerobic bacteria colonization. the peri-implant inflammatory tissue of bone defects from 12 edentulous patients with 18 unsuccessful implants (imz type) was removed after surgical opening of the defects. after grinding the tissue with glass beads in nutrient solution, an aliquot of the suspension was plated and incubated on appropriate culture media. the quantitative and qualitative di ...	1997	9048462
bacterial adherence to guided tissue regeneration barrier membranes exposed to the oral environment.	microbial colonization of barrier materials used in guided tissue regeneration (gtr) is known to adversely affect treatment outcomes. the purpose of this study was to compare the rate at which 11 commonly-occurring oral bacteria species colonize three different barrier materials (collagen, expanded polytetrafluoroethylene, and polylactic acid). the study group consisted of 10 systemically healthy individuals with no history of periodontal disease and absence of antimicrobial therapy within the p ...	1997	9058336
[vertebral osteomyelitis by prevotella melaninogenica, candida albicans and mycobacterium tuberculosis in a intravenous drug addict].		1997	9101742
cloning and characterization of a prevotella melaninogenica hemolysin.	hemolysins have been proven to be important virulence factors in many medically relevant pathogenic organisms. their production has also been implicated in the etiology of periodontal disease. hemolytic strain 361b of prevotella melaninogenica, a putative etiologic agent of periodontal disease, was used in this study. the cloning, sequencing, and characterization of phya, the structural gene for a p. melaninogenica hemolysin, is described. no extensive sequence homology could be identified betwe ...	1997	9199448
comparison of lipopolysaccharides from bacteroides, porphyromonas, prevotella, campylobacter and wolinella spp. by tricine-sds-page.	lipopolysaccharides (lpss) of 11 bacterial strains from the type species of the genera bacteroides (b. fragilis), prevotella (pr. melaninogenica), porphyromonas (po. gingivalis), campylobacter (c. fetus subsp. fetus), and wolinella (w. succinogenes), and from the type strains of b. distasonis, b. forsythus, b. ureolyticus, po. levii, po. macacae, and c. gracilis, were extracted with hot water-phenol (westphal method). s-form lpss, obtained from all organisms, were well resolved with tricine-sodi ...	1997	9206383
characterization of saccharolytic bacteroides and prevotella isolates from infected dog and cat bite wounds in humans.	saccharolytic, nonpigmented, anaerobic gram-negative rods isolated from infected dog and cat bite wounds in humans have been poorly characterized, and most are not included in the databases of kits used for anaerobic identification; thus, they are problematic for clinical laboratories to identify. fifty strains isolated from such wounds were characterized with commercial kits for preformed-enzyme detection, carbohydrate fermentation, and other biochemical tests. pcr fingerprinting was performed ...	1997	9003606
pulmonary mucormycosis in diabetic renal allograft recipients.	renal allograft recipients are prone to opportunistic infections due to their need of immunosuppression to prevent rejection. mucormycosis is a rare opportunistic infection caused by a fungi of the order mucorales. risk factors predisposing to this disease include prolonged neutropenia, chelation therapy for iron or aluminum overdose, diabetes, and patients who are immunosuppressed. life-threatening infections can occur, as this fungi has the propensity to invade blood vessel endothelium, result ...	1997	9041226
a hemagglutinating variant of prevotella melaninogenica isolated from the oral cavity.	strains resembling prevotella melaninogenica were isolated from healthy subjects and patients with periodontal disease and were identified using: a 5-test phenotypic screen; commercial identification kits; and a 16s rrna-based polymerase chain reaction (pcr) method. eleven clinical isolates closely resembling p. melaninogenica, and all from patients with periodontitis, were able to agglutinate erythrocytes. in the electron microscope, hemagglutinating isolates showed fimbria-like structures, tha ...	1998	9872112
detection of dextranase-producing gram-negative oral bacteria.	thirty-one strains of 23 gram-negative oral bacterial species were examined for dextran-degrading activity on agar plates containing blue dextran. one strain each of capnocytophaga ochracea, capnocytophaga sputigena, prevotella loescheii, prevotella melaninogenica and prevotella oralis had detectable dextranase activity. the culture supernatants of p. melaninogenica and p. oralis cells contained dextranases of multiple sizes, but those of the other three species had a single size of enzyme. a 56 ...	1998	9872116
oropharynx microbiota among alcoholics and non-alcoholics.	the oropharynx microbiota plays an important role in the origin of infections, especially among alcoholics whose airway defenses are impaired.	1998	9876451
[clinical evaluation of lung abscess diagnosed by transtracheal aspiration].	we have diagnosed lung abscess according to findings of infiltration with cavity formation on chest x-ray and/or ct-scan and pathogens isolated from transtracheal aspirates. we evaluated the clinical features of 20 patients with lung abscess (18 males and 2 females, mean age; 54.3 years). diabetus mellitus and periodontal diseases were prominent underlying diseases in patients with lung abscess. cough was complained in 13 patients, chest or back pain in 9, purulent sputum in 8 and hemosputum in ...	1998	9884505
inflammatory cytokine production and specific antibody responses against possible causative bacteria in patients with multilesional periapical periodontitis.	we examined the induction of inflammatory cytokines (including interleukin-1, interleukin-6, interleukin-8, and tumor necrosis factor-alpha) by several species of possible causative bacteria in periapical periodontitis. assays were done on human whole blood cultures from patients with differing numbers of periapical lesions; those having radiographically clear periapical lesions in 10 or more teeth (high lesion group), in one or two teeth (low lesion group), and healthy volunteers having no peri ...	1998	10023261
bacterial interference in the nasopharynx following antimicrobial therapy of acute otitis media.	the effect on the nasopharyngeal bacterial flora of therapy for 10 days with co-amoxiclav or cefprozil was studied in 50 children with acute otitis media. before therapy, potential pathogens (streptococcus pneumoniae, haemophilus influenzae and moraxella catarrhalis) were isolated in 14 (56%) of those treated with co-amoxiclav and 15 (60%) of those treated with cefprozil. following therapy, the reduction in the number of these pathogens was the same in the two groups. however, differences betwee ...	1998	9598781
recovery of anaerobic bacteria from a post-traumatic nasal septal abscess. a report of two cases.	this report presents 2 children (boys, 11 and 9 years old) who had post-traumatic nasal septal abscesses. cultures for aerobic and anaerobic bacteria revealed peptostreptococcuus magnus and a beta-lactamase-producing prevotella intermedia in the first patient, and peptostreptococcus anaerobius, streptococcus intermedius, and prevotella melaninogenica in the second patient. anaerobic bacteria may play a role in nasal septal abscesses.	1998	9823846
[microbiology of acute pharyngitis and acute tonsillitis and treatment with taileqi troches].	a qualitative analysis of microflora from pharynx of 100 cases of acute pharyngitis and acute tonsillitis was performed. these patients were randomly divided into two groups so as to assess treatment effect of taileqi troches vs amoxycillin. results showed that beta-hemolytic streptococcus, klebsiella pneumoniae, staphylococcus aureus were the most common organisms causing diseases. the predominant anaerobic organisms were bacteroides melaninogenicus, eptostrep. these findings suggest that anaer ...	1998	11189192
proteolysis and utilization of albumin by enrichment cultures of subgingival microbiota.	subgingival dental plaque consists mainly of microorganisms that derive their energy from amino acid fermentation. their nutrient requirements are met by the subgingival proteolytic system, which includes proteases from microorganism and inflammatory cells, and substrate proteins from sulcus exudate, including albumin. to determine the selective effect of individual proteins on microbiota, we used albumin as the main substrate for growth. eight subgingval plaque samples from untreated periodonta ...	1999	10895689
relationship of the subgingival microbiota to a chairside test for aspartate aminotransferase in gingival crevicular fluid.	the aim of the present study was to evaluate the association between the occurrence of certain specific periodontal pathogens and aspartate aminotransferase (ast) levels in gingival crevicular fluid (gcf).	1999	10052771
gastrointestinal case of the day. pyogenic liver abscess caused by perforation by a swallowed wooden toothpick.		1999	10336207
induction of oxidative dna damage in anaerobes.	we compared oxidative dna damage in strictly anaerobic prevotella melaninogenica, aerotolerant anaerobic bacteroides fragilis, and facultative anaerobic salmonella typhimurium after exposure to o2 or h2o2. using hplc with electrochemical detection, we measured 8-hydroxydeoxyguanosine (8ohdg) as a damage marker. o2 induced 8ohdg in p. melaninogenica but not in b. fragilis, which shows catalase activity, or in s. typhimurium. in p. melaninogenica, with catalase, o2 induced less 8ohdg; superoxide d ...	1999	10359070
beta-lactamase production by oral anaerobic gram-negative species in infants in relation to previous antimicrobial therapy.	the frequency of beta-lactamase production in gram-negative bacteria has increased considerably during recent years. in this study, beta-lactamase production by oral anaerobic gram-negative rods isolated from saliva was longitudinally examined for 44 caucasian infants at the ages of 2, 6, and 12 months in relation to their documented exposure to antibiotics. isolates showing decreased susceptibility to penicillin g (1 microg/ml) were examined for beta-lactamase production by using a chromogenic ...	1999	10390208
[trovafloxacin in gynecology].	presence of microorganisms in the female lower genital tract (lgt) in concentrations that modify the established normal equilibrium produce different symptoms that make necessary to consult the gynecologist. it is currently accepted that infections of the lgt are due to microorganisms that are normally integrating the internal flora, except for the erroneously called "sexually transmitted diseases" that are caused by external microorganisms. this means that all those microorganisms usually prese ...	1999	10436555
in vitro activity of gemifloxacin (sb 265805) against anaerobes.	gemifloxacin mesylate (sb 265805), a new fluoronaphthyridone, was tested against 359 recent clinical anaerobic isolates by the national committee for clinical laboratory standards reference agar dilution method with supplemented brucella blood agar and an inoculum of 10(5) cfu/spot. comparative antimicrobials tested included trovafloxacin, levofloxacin, grepafloxacin, sparfloxacin, sitafloxacin (du-6859a), penicillin g, amoxicillin clavulanate, imipenem, cefoxitin, clindamycin, and metronidazole ...	1999	10471570
[clinical evaluation of anaerobic infections in patients with bronchopulmonary infections diagnosed by transtracheal aspiration].	we evaluated the clinical and bacteriologic features in the patients with bronchopulmonary infections isolated anaerobes from transtracheal aspirates between april 1990 and march 1998. some anaerobe was isolated in 42 (10.9%) in 387 patients whom we performed transtracheal aspiration (tta), in 42 (15.7%) of 268 in whom some organism was isolated from tta, or in 42 (16.3%) of 257 patients in whom some bacterium excluding acid-fast bacteria, fungi or mycoplasma from tta. the isolation rate of anae ...	1999	10481403
establishment of oral anaerobes during the first year of life.	anaerobic species constitute a significant part of the bacterial community of the mouth. although the time and species involved in the primary colonization of infants are of great importance by forming the basis for further colonization, the development of the oral anaerobic microflora with age is still inadequately understood. in the present study, time and succession of colonization of oral anaerobes were longitudinally examined in 44 healthy caucasian infants at 2, 6, and 12 months of age. un ...	1999	10520968
association of black-pigmented bacteria with endodontic infections.	black-pigmented bacteria (bpb) have been associated with endodontic infections. the purpose of this study was to evaluate further the presence of bpb with the clinical signs and symptoms associated with endodontic infections. microbial samples were collected from the root canals of 40 intact teeth with necrotic pulps and apical periodontitis. conventional laboratory methods were used for identification of the strains of bpb isolated in pure culture. in addition, the polymerase chain reaction and ...	1999	10530240
an evaluation of the action of different root canal irrigants on facultative aerobic-anaerobic, obligate anaerobic, and microaerophilic bacteria.	the aim of this study was to test the effect of different concentrations of sodium hypochlorite, chlorhexidine, and cetrimide on the following bacterial strains: facultative aerobes-anaerobes: candida albicans atcc 10231; enterococcus faecalis atcc 29212; escherichia coli atcc 25,922; pseudomonas aeruginosa atcc 27,853; streptococcus mitis atcc 9811; streptococcus mutans atcc 35668; streptococcus salivarius atcc 13419; and streptococcus sanguis atcc 10556. microaerophiles: actinobacillus actinom ...	1999	10530260
activities of gemifloxacin (sb 265805, lb20304) compared to those of other oral antimicrobial agents against unusual anaerobes.	the activities of gemifloxacin (sb 265805, lb20304) and comparator agents were determined by an agar dilution method against 419 clinical strains of less-commonly identified species of anaerobes. gemifloxacin was generally more active than trovafloxacin against gram-positive strains by one to two dilutions. peptostreptococci (peptostreptococcus asaccharolyticus, peptostreptococcus magnus, peptostreptococcus micros, and peptostreptococcus prevotii) and porphyromonas spp. (porphyromonas asaccharol ...	1999	10543754
[anaerobic infection and its rapid detection in surgical patients].	to investigate anaerobic infection and its rapid detection in surgical patients.	1999	11829950
evaluation of the rapid id 32a system for identification of anaerobic gram-negative bacilli, excluding the bacteroides fragilis group.	objective: to evaluate the rapid id 32a system (biomérieux, marcy-l'etoile, france) for the identification of anaerobic gram-negative bacilli, excluding the bacteroides fragilis group. methods: five hundred and twenty-eight identified clinical isolates of non-b. fragilis group anaerobic gram-negative bacilli were tested in the rapid id 32a system, and identifications were compared with those obtained with conventional biochemical tests and gas-liquid chromatography. results: the rapid id 32a sys ...	1999	11856276
features of severe periodontal disease in a teenager with chédiak-higashi syndrome.	chédiak-higashi syndrome (c-hs) is a rare congenital disease characterized by defective neutrophil function with abnormal lysosomal inclusions, neutropenia, and reduced chemotaxis. the complete syndrome includes oculocutaneous albinism with photophobia, neurologic features, recurrent infections, and enterocolitis.	2000	10872965
"checkerboard" assessments of periodontal microbiota and serum antibody responses: a case-control study.	we explored the association between subgingival microbial profiles and serum igg responses to periodontal microbiota in relation to clinical periodontal status.	2000	10914791
differences in the composition of the subgingival microbiota of two periodontitis populations of different geographical origin. a comparison between spain and the netherlands.	the purpose of this study was to compare the subgingival microbiota of two geographically distinct patient populations using identical clinical and bacteriological methods. adult patients with a diagnosis of periodontitis were consecutively selected according to pre-defined clinical criteria. microbiological samples were taken from the deepest four sites with bleeding. the samples were plated on blood agar plates, for the determination of the total anaerobic counts and identification of specific ...	2000	11037754
mechanism of oxidative dna damage induction in a strict anaerobe, prevotella melaninogenica.	we investigated the mechanism of the oxidative dna damage induction by exposure to o(2) in prevotella melaninogenica, a strict anaerobe. flow cytometry with hydroethidine and dichlorofluorescein diacetate showed that o(2) exposure generated o(2)-) and h(2)o(2). results of electron spin resonance with alpha-(4-pyridyl-1-oxide)-n-tert-butylnitrone and ethanol showed that o(2) exposure also induced oh radical generation in p. melaninogenica loaded with fecl(2) but not in samples without fecl(2) l ...	2000	11040441
inhibition of actinobacillus actinomycetemcomitans leukotoxicity by bacteria from the subgingival flora.	actinobacillus actinomycetemcomitans produces a pore-forming leukotoxin that lyses human polymorphonuclear leukocytes and monocytes. certain proteolytic bacteria may coexist with a. actinomycetemcomitans in periodontal pockets. we aimed therefore to examine whether oral bacteria can modify the leukotoxicity of a. actinomycetemcomitans. a total of 55 strains representing 45 bacterial species of the subgingival flora were tested. each strain was incubated with the highly toxic strain of a. actinom ...	2000	11154406
profile of subgingival microbiota in children with mixed dentition.	a diversity of microbial species has been detected in children's oral flora at an early age. to investigate the composition of the subgingival microbiota of different groups of teeth in children with mixed dentition, 40 systemically healthy children, aged 7-8 years, randomly chosen, were examined. subgingival plaque samples were taken from the mesiobuccal sites of 21, 41, 16 and 36 permanent teeth and 53, 73, 64 and 84 deciduous teeth. the samples were cultured for bacterial isolation anaerobica ...	2000	11155173
[antilysozyme activity of anaerobic bacteria from fecal microflora in man].	for the first time anaerobic bacteria of the fecal microflora in man have been found be capable of inactivating lysozyme. the presence of this antilysozyme sign has been noted in both gram-positive anaerobes (bifidobacterium, propionibacterium, eubacterium, actinomyces israelii) and in gram-negative anaerobes (bacteroids, prevotella melaninogenica). the expression of antilysozyme activity in the anaerobes under study has been determined. the possible biological role of this sign of the indigenou ...	2000	11220957
cleavage of a recombinant human immunoglobulin a2 (iga2)-iga1 hybrid antibody by certain bacterial iga1 proteases.	to understand more about the factors influencing the cleavage of immunoglobulin a1 (iga1) by microbial iga1 proteases, a recombinant human iga2/iga1 hybrid molecule was generated. in the hybrid, termed iga2/a1 half hinge, a seven-amino-acid sequence corresponding to one half of the duplicated sequence making up the iga1 hinge was incorporated into the equivalent site in iga2. insertion of the iga1 half hinge into iga2 did not affect antigen binding capacity or the functional activity of the hybr ...	2000	10639405
[the dynamics of the colonization by the microbial flora of the mouth of the different materials used for dental prosthetics].	colonization of materials used for making dentures with oral microflora was studied in 12 patients with chronic generalized medium severe periodontitis during remission. time course of dentures colonization with representatives of oral microflora (s. sanguis, peptostreptococcus sp., prevotella oralis) and with bacteria pathogenic for the periodontium (prevotella melaninogenica, a. naeslundii, fusobacterium sp.) depends on the material from which the dentures are made. minimal colonization of bac ...	2000	10693351
pyomyositis of the thigh due to prevotella melaninogenica.	pyomyositis is an uncommon infection in temperate climates, however, it is being more frequently reported among patients with diabetes or malignancy, or those who are immunocompromised. it is predominantly caused by staphylococcus aureus, and rarely by bacteroides species. pyomyositis due to prevotella melaninogenica has not previously been reported. we describe an elderly patient with pyomyositis of the thigh due to p. melaninogenica which was successfully treated by surgical incision and drain ...	2000	10697793
detection of tetq and ermf antibiotic resistance genes in prevotella and porphyromonas isolates from clinical specimens and resident microbiota of humans.	gram-negative anaerobes belonging to the genera fusobacterium, prevotella and porphyromonas were investigated for the presence of tetq and ermf, which have been shown to be spread by conjugal elements. one hundred isolates from either sites of infection or various body sites in healthy subjects were studied. pcr was used to detect tetq, and dna-dna hybridization studies on ecori chromosomal digests were undertaken to detect the presence of tetq and ermf. antibiotic sensitivity assays were perfor ...	2000	10797077
recovery of anaerobic bacteria from 3 patients with infection at a pierced body site.	we describe 3 adolescents who developed infections due to anaerobes at pierced body sites: the nipple, the umbilicus, and the nasal septum. anaerobes (prevotella intermedia and peptostreptococcus anaerobius) were recovered from pure culture of specimens obtained from 1 patient with nipple infection and were mixed with aerobic bacteria in cultures of specimens obtained from 2 patients (streptococcus aureus, peptostreptococcus micros, and prevotella melaninogenica were recovered from a patient wit ...	2001	11389513
inhibitory effect of lactoperoxidase-generated hypothiocyanite upon black pigmented anaerobe growth.	this study aimed to evaluate the in vitro effect of lactoperoxidase with or without its substrates (hydrogen peroxide, thiocyanate) on the growth of 4 different black pigmented anaerobe (bpa) strains associated with the development and progress of periodontal diseases: porphyromonas gingivalis atcc 33277, prevotella intermedia nctc 9336, prevotella loescheii atcc 15930, and prevotella melaninogenica nctc 9338. a 5-min lactoperoxidase-generated oscn--hoscn incubation at ph 6.0, 7.0 or 8.0 resulte ...	2001	11408950
[study of bacteriology on local application of tinidazole stilus in treatment for periodontitis].	the study was designed to evaluate the antiseptic efficacy of tinidazole stilus against periodontal pathogen.	2001	12539636
[effect of macrolide therapy on microbes in nasal cavity and larynx of patients with chronic paranasal sinusitis].		2001	12575431
study of fungal and bacterial infections of the diabetic foot.	microbiological study for aerobic organisms, anaerobic organisms and fungi from 105 cases of diabetic foot ulcers was carried out to determine the aetiological agents and their antibiograms. out of 265 microbial isolates obtained, 160 were aerobes, 50 anaerobes and 55 fungal strains. polymicrobial infection was observed in 73 (69.5%) cases. the most frequently isolated aerobic microorganisms were staphylococcus aureus and pseudomonas aeruginosa. among the anaerobes bacteroides melaninogenicus an ...	2002	12593559
quantitative microbiological study of human carious dentine by culture and real-time pcr: association of anaerobes with histopathological changes in chronic pulpitis.	the bacteria found in carious dentine were correlated with the tissue response of the dental pulps of 65 teeth extracted from patients with advanced caries and pulpitis. standardized homogenates of carious dentine were plated onto selective and nonselective media under anaerobic and microaerophilic conditions. in addition, real-time pcr was used to quantify the recovery of anaerobic bacteria. primers and fluorogenic probes were designed to detect the total anaerobic microbial load, the genera pr ...	2002	11980945
[portal vein thrombosis and prevotella melanigenica revealing an appendicular abscess].	the misleading aspects of appendicitis are multiple. we report an observation, original not only from a clinical and bacteriological point of view but also because of the presence of a portal vein thrombosis.	2002	11984983
bacterial and fungal microleakage of ah26 and ah plus root canal sealers.	to evaluate the penetration of candida albicans alone and a combination of bacteria through root canals filled with gutta-percha and one or other root canal sealers, ah26 and ah plus.	2002	12059913
salivary microbiota levels in relation to periodontal status, experience of caries and miswak use in sudanese adults.	the purpose of the present investigation was to assess the salivary levels of 25 oral bacteria in relation to periodontal status and experience of caries, and to compare the levels of these bacteria between habitual miswak and toothbrush users in adult sudanese subjects.	2002	12060423
frequency of reactivity for porphyromonas gingivalis and prevotella spp. in supra- and subgingival plaques, and periodontal clinical parameters according to subject age.	the present study was conducted to assess the association between selected clinical parameters and the distribution of porphyromonas gingivalis (pg), prevotella intermedia (pi), prevotella nigrescens (pn), and prevotella melaninogenica (pm) in supra- and subgingival plaque samples measured by an immunoslot blot assay (iba) using their monoclonal antibodies.	2002	12211497
recovery of anaerobic bacteria from a glossal abscess in an adolescent.	glossal abscess is infrequent in children. anaerobic bacteria are rarely recovered from this infection and never have been reported in children or adolescents. a 15-year-old patient presented with a tongue abscess following trauma. aspirate of the abscess yielded polymicrobial anaerobic flora: prevotella melaninogenica, fusobacterium nucleatum, and peptostreptococcus micros. the patient recovered following incision and drainage and 14 days of antimicrobial therapy with clindamycin. this report i ...	2002	12395007
a study on the isolation of anaerobic bacteria from the root canal infections in anterior non-vital teeth with special reference to bacteroides species.		2002	12420575
suppurative parotitis caused by anaerobic bacteria in newborns.	staphylococci are the usual bacterial etiology of suppurative parotitis in newborns. this report describes for the first time recovery of anaerobic bacteria from aspirates of the infected gland in two infants with suppurative parotitis. peptostreptococcus intermedius and prevotella melaninogenica were isolated from one child and prevotella intermedia from the other patient. complete recovery occurred after 4 weeks of antimicrobial therapy.	2002	11791111
faropenem, a new oral penem: antibacterial activity against selected anaerobic and fastidious periodontal isolates.	the in vitro activity of faropenem, an oral penem, was compared with those of penicillin, co-amoxiclav, cefoxitin, clindamycin, erythromycin and metronidazole against 106 isolates of anaerobic pathogens involved in systemic infections. the organisms tested comprised porphyromonas gingivalis (29), prevotella spp. (eight), prevotella melaninogenica (seven), prevotella intermedia (five), actinomyces spp. (25), fusobacterium nucleatum (14), peptostreptococcus spp. (11), bacteroides ureolyticus (five ...	2003	12615878
beta-lactamase production in prevotella and in vitro susceptibilities to selected beta-lactam antibiotics [corrected].	this study looked for beta-lactamase production in 100 prevotella isolates. mics were determined for amoxycillin, ticarcillin, amoxycillin+clavulanate, cephalothin, cefuroxime, cefixime, cefpodoxime and cefotaxime using the reference agar dilution method (standard m11 a4, nccls). beta-lactamase activity was detected in 58 of the 100 isolates, 24 of 46 black-pigmented provotella and 34 of 54 non-pigmented prevotella. all beta-lactamase-negative strains were susceptible to all beta-lactam antibiot ...	2003	12636990
bactericidal activities of methoxyfluoroquinolones gatifloxacin and moxifloxacin against aerobic and anaerobic respiratory pathogens in serum.	gatifloxacin (bristol-myers squibb) and moxifloxacin (bayer) are new methoxyfluoroquinolones with broad-spectrum activity against aerobic and anaerobic pathogens of the respiratory tract. in this investigation, we analyzed the bactericidal activity in serum over time of these antimicrobials against three aerobic (streptococcus pneumoniae, haemophilus influenzae, and staphylococcus aureus) and four anaerobic (peptostreptococcus micros, peptostreptococcus magnus, fusobacterium nucleatum, and prevo ...	2003	12654663
[early diagnosis of bacterial brain abscesses: interest of diffusion-weighted mri].	three cases of bacterial brain abscesses, in immunocompetent patients, are reported. in all these cases, the diffusion-weighted magnetic resonance (mri) with apparent diffusion coefficient (adc) map has permitted an early diagnosis and a rapid treatment. this emergency mri showed in the three cases a low signal on ti-weighted images, a high signal on t2-weighted and echo-planar images, and a decrease of adc (0.36- 0.49 x 10(-3) mm2/s). so, this new mri technique provides an available and rapid e ...	2003	12773871
distribution of selected bacterial species on intraoral surfaces.	to examine the proportions of 40 bacterial species in samples from 8 oral soft tissue surfaces and saliva in systemically healthy adult subjects and to compare these microbiotas with those of supra- and subgingival plaque.	2003	12834503
identification of prevotella in pedal osteomyelitis of a diabetic patient.	osteomyelitis in a diabetic patient with a nonhealing foot ulcer, multiple medical conditions, and recurrent hospitalization for antibiotic therapy was found to be associated with gram-negative bacteria prevotella melanginoganica and prevotella melaninoganica hemagglutinating variant. those organisms were identified due to the morphologically distinct features in electron microscopy and sequencing of the genes after polymerase chain reaction amplification from the pathological material. the bact ...	2003	12907373
sensitivity of genera porphyromonas and prevotella to the bactericidal action of c-terminal domain of human cap18 and its analogues.	this paper reports the effect of the synthesized 27-amino acid sequence in the c-terminal domain of human cap18 (hcap18), a human cationic antibacterial protein or cathelicidin, on certain strains belonging to the genera porophyromonas and prevotella. the domain binds lipopolysaccharides (lps) from porophyromonas gingivalis and porophyromonas circumdentaria as well as enterobacterial lps. two analogues of hcap18, designated ll/cap18 and ff/cap18, were also tested to determine whether additional ...	2003	12930528
[modifying action of oxytocin on the biological properties of the causative agents of anaerobic non-clostridial infection].	in a number of in vitro experiments the effect of oxytocin on the antilysozyme and anticomplemental activity of propiobacterium propionicum, bacteroides fragilis, prevotella melaninogenica and peptostreprtococcus anaerobius, isolated from patients with acute pyoinflammatory pleuropulmonary diseases, was studied. antibiotic resistance dynamics of the infective agents under study to lincomycin, clindamycin, thienam, vancomycin was also detected. the inhibiting activity of oxytocin on the persisten ...	2003	12966884
[effect of a polyoxydonium immunoregulator on the biological properties of microorganisms].	the effect of the synthetic immunomodulator polyoxydonium (po) on some biological properties of pathogenic bacteria (shigella flexneri, salmonella enteritidis), opportunistic bacteria (klebsiella pneumoniae, escherichia coli, pseudomonas aeruginosa, staphylococcus aureus, bacteroides fragilis, peptostreptococcus anaerobius, prevotella melaninogenica, propionibacterium propionicum, clostridium difficile) and fungi (candida albicans), isolated during enteric infections, enteric dysbiosis, pyoinfla ...	2003	12966885
clinical presentation and bacteriologic analysis of infected human bites in patients presenting to emergency departments.	previous studies of infected human bites have been limited by small numbers of patients and suboptimal microbiologic methodology. we conducted a multicenter prospective study of 50 patients with infected human bites. seventy percent of the patients and assailants were young adult men. fifty-six percent of injuries were clenched-fist injuries and 44% were occlusional bites. most injuries were to the hands. fifty-four percent of patients were hospitalized. the median number of isolates per wound c ...	2003	14614671
role of anaerobes in acute pelvic inflammatory disease.	pouch of douglas aspirates were collected from 50 women with history and examination suggestive of acute pelvic inflammatory disease (pid) and 20 healthy women admitted for tubal ligation served as control. a total of 57 microorganisms were isolated from 37 patients out of 50 in study group. of 37 positive cultures 21(56.7%) were monomicrobial and 16(43.2%) were polymicrobial. most common symptom in study group was lower abdominal pain (90%), vaginal discharge (70%) and irregular bleeding (40%) ...	2003	17643017
an in vitro time-kill assessment of linezolid and anaerobic bacteria.	linezolid is a novel oxazolidinone antibacterial agent active against staphylococci (including methicillin-resistant strains), enterococci (including vancomycin-resistant strains), streptococci (including penicillin-intermediate and -resistant streptococcus pneumoniae), and other aerobic and facultative bacteria. the agent has also demonstrated activity against a broad spectrum of gram-positive and gram-negative anaerobic bacteria. previous time-kill assessments have shown linezolid to be genera ...	2003	16887680
[prevotella species involved in the onset of superficial face and neck abscesses].	the aim of the study was to identify at species level the prevotella strains from 30 pus samples collected by needle aspiration in patients with abscesses of fascial spaces of the face and neck. microscopy of gram-stained direct smear and cultures on selective and nonselective media incubated aerobically and anaerobically were performed in each pus specimen. all strains of gram-negative strictly anaerobic bacilli were identified to genus and species level using conventional methods of diagnosis ...	2004	16938935
anaerobic liver abscesses as initial presentation of silent colonic cancer.	rarely, multiple liver abscesses caused by anaerobic organisms are the presenting form of unsuspected colonic cancer in the absence of liver metastases.	2004	18333045
soft-tissue abscess involving actinomyces odontolyticus and two prevotella species in an intravenous drug abuser.	skin and soft-tissue infections in intravenous users comprise a variety of microorganisms and anaerobic bacteria are frequently involved in these suppurative infections. a case of subcutaneous abscess into anterior femoral muscles involving actinomyces odontolyticus and two prevotella species (prevotella buccae and prevotella melaninogenica) in an intravenous drug abuser is presented. this combination of microorganisms has not previously been described in soft-tissue infections. the patient volu ...	2004	14690717
effect of intrauterine administration of oestradiol on postpartum uterine bacterial infection in cattle.	after parturition fewer first dominant follicles are selected in the ovary ipsilateral to the previously gravid uterine horn in cattle. however, the presence of a large oestradiol-secreting follicle in the ipsilateral ovary is a predictor of fertility, possibly due to a localised effect of oestradiol which increases the rate of elimination of the ubiquitous uterine bacterial contamination that occurs after calving. the present study tested the hypothesis that oestradiol reduces uterine bacterial ...	2004	14749045
comparison of systemic flurithromycin therapy and clinical procedures in the treatment of periodontal diseases.	the purpose of the present investigation was to evaluate, in 20 periodontal patients, the microbial and clinical effects of flurithromycin therapy plus scaling and root planning (srp) in comparison with srp alone. clinical assessments of plaque, bleeding on probing and pocket depth were made prior to srp alone and srp plus flurithromycin therapy (375 mg twice daily for 5 days) and after both treatments. subgingival plaque samples (n. 180) were taken prior to and after both treatments and analyze ...	2004	15216949
relationship between volatile sulphide compounds concentration and oral bacteria species detection in the elderly.	to evaluate the relationship between oral bacteria species detection and volatile sulphide compounds (vsc) concentration in the elderly.	2004	15218895
subgingival microbiota of chronic periodontitis subjects from different geographic locations.	most clinical studies assume that the subgingival microbiota is similar from one geographic location to another. the purpose of the present investigation was to examine the composition of the subgingival microbiota in chronic periodontitis subjects from four countries.	2004	15491316
susceptibility of some oral microorganisms to chlorhexidine and paramonochlorophenol.	since the use of antimicrobial agents is required in endodontic therapies, this study aimed at determining the minimum inhibitory concentrations (mics) of chlorhexidine digluconate and paramonochlorophenol (pmc) against microorganisms commonly found in endodontic infections. both agents were tested by agar dilution tests against pseudomonas aeruginosa, staphylococcus aureus, enterococcus faecalis, escherichia coli, candida albicans, prevotella intermedia, porphyromonas gingivalis, porphyromonas ...	2004	15619879
differences in the subgingival microbiota of swedish and usa subjects who were periodontally healthy or exhibited minimal periodontal disease.	previous studies have shown differences in the mean proportions of subgingival species in samples from periodontitis subjects in different countries, which may relate to differences in diet, genetics, disease susceptibility and manifestation. the purpose of the present investigation was to determine whether there were differences in the subgingival microbiotas of swedish and american subjects who exhibited periodontal health or minimal periodontal disease.	2005	15642056
development and characterization of a simple perfused oral microcosm.	to validate perfused, inline, filter-based fermentation systems (multiple sorbarod devices, msd) for their ability to maintain stable oral bacterial communities. msd enable replicate (n=5) microcosm biofilms (bf) to be established and sampled, together with their perfusates (pa, cells in eluted medium).	2005	15715865
antimicrobial resistance and beta-lactamase production of clinical isolates of prevotella and porphyromonas species.	this study determined the beta-lactamase production and the antimicrobial resistance of 72 prevotella species and 48 porphyromonas species isolated from different clinical specimens.	2005	15722627
vertebral osteomyelitis caused by prevotella (bacteroides) melaninogenicus.	a 35-year-old, previously healthy female presented with severe low back pain, fever, and a high erythrocyte sedimentation rate 1 week after a routine dental cleaning. technetium-labeled leukocyte scanning and magnetic resonance imaging scan of the spine were negative for osteomyelitis. the patient underwent biopsy, cultures from which grew prevotella (bacteroides) melaninogenicus. appropriate antibiotic therapy resulted in resolution of symptoms. p. melaninogenicus is a gram-negative anaerobic b ...	2005	15759955
dual role of vitamin c in an oxygen-sensitive system: discrepancy between dna damage and cell death.	although vitamin c is considered to act both as pro-oxidant and antioxidant, the mechanisms underlying these actions are still unclear. using the oxygen-sensitive system of a strict anaerobe, prevotella melaninogenica, we investigated both the pro-oxidant and antioxidant mechanisms of vitamin c. in the presence of vitamin c, the 8-hydroxydeoxyguanosine (8ohdg) formation induced by oxygen exposure was enhanced, probably due to the action of vitamin c on hydrogen peroxide generated during oxygen e ...	2005	15763969
phototargeting oral black-pigmented bacteria.	we have found that broadband light (380 to 520 nm) rapidly and selectively kills oral black-pigmented bacteria (bpb) in pure cultures and in dental plaque samples obtained from human subjects with chronic periodontitis. we hypothesize that this killing effect is a result of light excitation of their endogenous porphyrins. cultures of prevotella intermedia and p. nigrescens were killed by 4.2 j/cm2, whereas p. melaninogenica required 21 j/cm2. exposure to light with a fluence of 42 j/cm2 produced ...	2005	15793117
the salivary microbiota as a diagnostic indicator of oral cancer: a descriptive, non-randomized study of cancer-free and oral squamous cell carcinoma subjects.	the purpose of the present investigation was to determine if the salivary counts of 40 common oral bacteria in subjects with an oral squamous cell carcinoma (oscc) lesion would differ from those found in cancer-free (oscc-free) controls.	2005	15987522
prevotella massiliensis sp. nov. isolated from human blood.	we report a bacterial isolate (marseille isolate) recovered from the blood of a patient hospitalized in an intensive care unit, presenting with severe trauma, fever and mechanical ventilation. colonies appeared at 37 degrees c on blood agar after 72 h incubation. this isolate was a strictly anaerobic, gram-negative rod phenotypically related to other prevotella species described to date: non-motile, catalase-negative, oxidase-positive, non-glucose fermenting, resistant to vancomycin and suscepti ...	2005	16085394
antibacterial activity of aqueous extracts of selected chewing sticks.	this aim of this study was to determine the antibacterial activity in extracts obtained from various nigerian chewing sticks. aqueous extracts from seventeen chewing sticks and the fruit of c. ferruginea, one fruit used in oral hygiene in nigeria, were screened for antibacterial activity against type cultures of staphylococcus aureus, bacillus subtilis, escherichia coli, and pseudomonas aeruginosa. eleven of the test extracts showed activity against at least two of these referenced organisms. mi ...	2005	16127476
human granulocytic anaplasmosis affecting the myocardium.	a case of 65-year-old male is reported who presented with myalgias, headache, and fever. he subsequently developed myocarditis and was diagnosed to have anaplasmosis on peripheral blood smear. he was treated with doxycycline for 30 days. a coronary angiogram done after recovery showed normal epicardial arteries. the case illustrates the importance of a careful examination of the peripheral smear, with a high index of clinical suspicion, which led to prompt treatment and complete recovery of the ...	2005	16191146