Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| coordinate regulation of the levels of type iii and type i collagen mrna in most but not all mouse fibroblasts. | a mouse genomic clone was isolated by cross-hybridization with a dna fragment which codes for the nh2-propeptide of chick alpha1(iii) collagen. the region of cross-hybridization within the mouse clone was localized, its sequence determined, and an exon coding for the nh2-propeptide of mouse alpha1(iii) collagen was identified. this dna fragment hybridizes to an rna species of approximately 5300 nucleotides, slightly larger than the major alpha2(i) collagen rna species. the mouse type iii collage ... | 1985 | 2981217 |
| molecular basis of host range variation in avian retroviruses. | previous genetic analysis has localized the region of the rous sarcoma virus (rsv) env gene responsible for host range specificity to that encoding the middle one-third of gp85. to better understand the host range determinants, the relevant regions of the genomes of infectious molecular clones of the transformation-defective prague strain of rsv, subgroup b (pr-rsv-b) and rous-associated virus 0 (rav-0) (subgroup e) were sequenced and compared with the sequence of pr-rsv-c. this comparative anal ... | 1985 | 2981357 |
| highly specific antibody to rous sarcoma virus src gene product recognizes a novel population of pp60v-src and pp60c-src molecules. | antiserum to the rous sarcoma virus (rsv)-transforming protein, pp60v-src, was produced in rabbits immunized with p60 expressed in escherichia coli. alpha p60 serum immunoprecipitated quantitatively more pp60v-src than did tumor-bearing rabbit (tbr) sera. when rsv-transformed cell lysates were preadsorbed with tbr serum, the remaining lysate contained additional pp60v-src, which was recognized only by reimmunoprecipitation with alpha p60 serum and not by tbr serum. in subcellular fractions of rs ... | 1985 | 2981886 |
| antiviral effect of flavonoids on human viruses. | the effect of several naturally occurring dietary flavonoids including quercetin, naringin, hesperetin, and catechin on the infectivity and replication of herpes simplex virus type 1 (hsv-1), polio-virus type 1, parainfluenza virus type 3 (pf-3), and respiratory syncytial virus (rsv) was studied in vitro in cell culture monolayers employing the technique of viral plaque reduction. quercetin caused a concentration-dependent reduction in the infectivity of each virus. in addition, it reduced intra ... | 1985 | 2981979 |
| a retrovirus-like strategy for expression of a fusion protein encoded by yeast transposon ty1. | eukaryotic transposons such as the ty element of yeast or the copia-like sequences of drosophila show structural and functional similarities to both prokaryotic transposons and retroviral proviruses, but the prokaryotic transposons and retroviral proviruses use markedly different expression strategies which yield products having entirely different functions. to determine the phylogenetic relationship between eukaryotic transposons, prokaryotic transposons and retroviruses, we have sought to iden ... | 1985 | 2982101 |
| transfer of genes into hematopoietic cells using recombinant dna viruses. | the ability of recombinant dna viruses to transfer genes into hematopoietic cells has been explored. a recombinant simian virus 40 (sv40) in which the early region had been replaced with the chloramphenicol acetyltransferase (cat) gene driven by the promoter from rous sarcoma virus (rsv), was constructed. this virus transferred the cat gene more efficiently into mouse and human bone marrow cells and into the k562, mel, and wehi hematopoietic tissue culture cell lines, than the classical calcium ... | 1985 | 2982141 |
| distinctive effects of the viral oncogenes myc, erb, fps, and src on the differentiation program of quail myogenic cells. | the relationship between susceptibility to transformation in vitro by different oncogenes and terminal differentiation was analyzed in embryonic quail myogenic cells. infection with rous sarcoma virus (rsv), fujinami sarcoma virus (fsv), avian erythroblastosis virus (aev), and the avian myelocytomatosis virus mc29 led to rapid and massive transformation. transformed cells had distinctive morphological alterations, increased proliferation rates, and the ability to grow in agar suspension. further ... | 1985 | 2982156 |
| transformation of erythroid cells by rous sarcoma virus (rsv). | rsv transforms several nonhematopoietic cell types and as reported here also has the capacity to transform hematopoietic cells of the erythroid lineage. in vitro, the three rsv isolates tested induced erythroblast-like colonies in infected bone marrow cells that were distinguishable by size and cell arrangement from those induced by avian erythroblastosis virus (aev). also in contrast to aev-transformed erythroblast cultures, isolated cell colonies induced by rsv required complex growth conditio ... | 1985 | 2982235 |
| organization of cytoskeleton and fibronectin matrix in rous sarcoma virus (rsv)-transformed fibroblast lines with different metastatic potential. | metastatic clones growing in 0.6% 'hard' agar were selected from the non-metastatic rous sarcoma virus (rsv)-transformed tumorigenic b77-3t3 mouse fibroblast line. the incidence of spontaneous lung metastases varied among clones around 100%, while it was lower than 5% in the parental tumor line. the organization of microfilaments, microtubules and intermediate filaments as well as the pattern of extracellular fibronectin matrix were analyzed by immunofluorescence in two representative clones (b7 ... | 1985 | 2982623 |
| rsv provirus with same flanking sequences is found on different size classes of chinese hamster chromosomes. | rous sarcoma virus(rsv)-transformed chinese hamster fibroblasts, containing approximately ten copies of the dna domain comprising a single provirus and its flanking cellular sequences, were arrested in metaphase, and the chromosomes were fractionated by size in a sucrose gradient. the resolution of polymorphic ribosomal genes, the dihydrofolate reductase gene, and the c-src gene demonstrated that the gradient can distinguish between small, medium, and large chromosomes. the same dna domain carry ... | 1985 | 2982758 |
| rous sarcoma virus src gene expression on the growth of quail embryo skin fibroblasts and the establishment of permanent cell lines. | permanent cell lines of quail embryo fibroblasts appear in cultures of cells infected with a wild type strain of rous sarcoma virus (sr-rsv) or with its temperature sensitive transformation mutants (ts-t) (nyts68 and pa101) following a three step process. in step one, infected cells grow twice as fast as the control. the second step consists of a crisis during which the cell population is stationary for four to five weeks. towards the fourth week several foci of cell growth are observed in the f ... | 1985 | 2982767 |
| identification of respiratory virus antigens in middle ear fluids of children with acute otitis media. | antigens of respiratory viruses were tested by immunoassay of the middle ear fluids and nasopharyngeal secretions of 137 children with acute otitis media. the following were found: (1) an epidemic of infection with respiratory syncytial virus (rsv) caused a significant increase in the occurrence of acute otitis media. (2) fifteen percent of the children had rsv antigens in middle ear fluid, and in 7% rsv was the sole pathogen found. adenovirus antigens were found in the middle ear fluid of 3% of ... | 1985 | 2982961 |
| activation of c-myc gene transcription by rous sarcoma virus infection. | transcription of the c-myc gene in chick embryo fibroblast cells was activated 50- to 100-fold by rous sarcoma virus (rsv) infection. more than 30-fold activation of c-myc gene transcription was also observed in rsv-transformed rat cells as compared with the control cells. the increased expression of c-myc gene in these cells was not due to gene amplification or gene rearrangement. this striking increase in expression of c-myc gene may be relevant to neoplastic transformation of rsv-infected cel ... | 1985 | 2984071 |
| deletion in the 3' pol sequence correlates with aberration of rna expression in certain replication-defective avian sarcoma viruses. | the rna expression of a series of replication-defective recovered avian sarcoma viruses (rasvs) were studied. abnormal-sized viral rnas, both larger and smaller than the genome, were observed in the nonproducer cells infected with rasvs containing env and pol deletions. each nonproducer clone contained a single provirus integrated at a unique site and expressed a unique rna pattern. upon rescuing of the sarcoma virus with a helper virus and subsequent cloning, the rna pattern of individual nonpr ... | 1985 | 2985807 |
| no expression of a rous sarcoma virus-induced tumor antigen in mammalian cells infected with retroviruses transducing other oncogenes of the src gene family. | immunization with mouse and rat cells transformed by rous sarcoma virus (rsv) or by b77 avian sarcoma virus (asv) induced complete transplantation resistance against an rsv-induced mouse tumor (csa1m) in syngeneic hosts. in contrast, most of the mice immunized with a fujinami sarcoma virus-transformed rat fibroblast line (fsv-3y1), a feline sarcoma virus-transformed cat fibroblast line (fesv-fef), an abelson leukemia virus-infected balb/3t3 cell line (ablv-3t3), or an uninfected 3y1 cell line co ... | 1985 | 2985859 |
| parabiosis between avian embryos resistant and susceptible to rous sarcoma virus. | parabiosis of chicken embryos was used to determine if resistance or susceptibility to rous sarcoma virus (rsv) tumors could be transferred between rsv-resistant (r) and rsv-susceptible (s) embryos. eggs from rsv-r and rsv-s lines were parabiosed at 12 days of incubation. one week after hatching the chicks were inoculated in the wing web with rsv. birds were examined for tumor lesions through 9 weeks of age. lesion scores of the nonparabiosed rsv-s line were significantly higher than nonparabios ... | 1985 | 2986089 |
| rearrangements of viral and cellular dna are often associated with expression of rous sarcoma virus in rat cells. | rous sarcoma virus (rsv) proviruses integrated within the dna of transformed rat cells frequently display duplications of variable segments of proviral dna upstream of an intact provirus. the rearrangement in the a11 clone of transformed rat cells consists of a partial duplication of both viral and cellular dna segments whose origin is a region of approximately 4 kb encompassing the 3' virus-cell junction. transposition of this dna appears to have occurred at or after virus integration by a mech ... | 1985 | 2986846 |
| virus - tumor cell relationships. in vivo cocultivation of para-influenza type 1 (sendai) virus and of rous sarcoma virus (schmidt-ruppin strain) in mouse ehrlich ascites carcinoma. | co-infection of ehrlich ascites carcinoma (eac)-bearing mice with sendai virus and rous sarcoma virus (rsv) did not result in the formation of complete rsv. sendai virus could be, however, propagated in this system over 8 serial passages. as demonstrated by immunofluorescence and complement fixation reactions, antigens specific to each virus were synthesized in eac cells following either single or mixed virus infection. the virus progens also contained antigenic fractions incorporated from the h ... | 1985 | 2988191 |
| the location of cis-acting regulatory sequences in the human t cell lymphotropic virus type iii (htlv-iii/lav) long terminal repeat. | the location of cis-acting regulatory sequences within the long terminal repeat (ltr) of the human t cell lymphotropic virus type iii (htlv-iii/lav) was determined. an enhancer element capable of increasing the rate of transcription from a heterologous promoter, irrespective of distance and orientation, is located between nucleotides -137 and -17 (cap site +1). the promoter sequences present near the tata box respond to heterologous enhancers. the sequences present between nucleotides -17 and +8 ... | 1985 | 2988790 |
| change of hyaluronic acid synthesis during differentiation of myogenic cells and its relation to transformation of myoblasts by rous sarcoma virus. | hyaluronic acid synthesis was examined in cultures of differentiating chick embryo muscle cells before, during and after fusion. prior to fusion, hyaluronic acid was synthesized and secreted into the medium, but once fusion began this synthesis was reduced significantly. synthesis then increased again after completion of fusion. thus, production of hyaluronic acid was lowest at the time of or right before cell fusion. when myoblasts were transformed by rous sarcoma virus (rsv), a higher amount o ... | 1985 | 2988797 |
| strain differences in the antitumor activity of an immunopotentiator, nocardia rubra cell-wall skeleton, in b10 congenic and recombinant mice. | an immunogenetic evaluation of the antitumor activity of the immunopotentiator nocardia rubra cell-wall skeleton (n. rubra-cws) has been performed using non-virus-producing tumors induced by the schmidt-ruppin strain of rous sarcoma virus (rsv) in b10 congenic and recombinant mice. live tumor cells mixed with either n. rubra-cws or a placebo control were inoculated intradermally into the right flank of syngeneic mice. with n. rubra-cws, the development and growth of b10 mouse tumors, s1018(b10) ... | 1985 | 2989065 |
| surveillance of respiratory viral infections by rapid immunofluorescence diagnosis, with emphasis on the epidemiological development of respiratory syncytial virus infections. | surveillance of certain respiratory viral infections by applying immunofluorescence (if) examinations to samples of nasopharyngeal secretions has been evaluated using a simplified procedure for the preparation of cell smears. samples from 711 children living in different parts of norway were examined during the winter 1982/83 and a positive diagnosis was made for 290 children (41%). temporal epidemic peaks were observed for respiratory syncytial virus (rsv), parainfluenza virus type 3 and influe ... | 1985 | 2989359 |
| local degradation of fibronectin at sites of expression of the transforming gene product pp60src. | local degradation of extracellular fibronectin, a major extracellular adhesive protein, is believed to play an important part in the migration of cells through the extracellular matrix during tumour invasion, morphogenetic movement and trophoblast implantation. fibronectin is lost from the cell surface after transformation with rous sarcoma virus (rsv). by using fluorescent and radiolabelled probes covalently coupled to the surface of substrata, we have recently identified a proteolytic activity ... | 1985 | 2989711 |
| the chromatin structure of rous sarcoma proviruses is changed by factors that act in trans in cell hybrids. | in several lines of rous sarcoma virus (rsv)-transformed rat cells the proviruses are in a configuration typical of active eukaryotic genes. they are sensitive to pancreatic dnase i, with sites hypersensitive to nuclease near the 5' end of the genome, they are close to the nuclear 'cage' and they show a low level of cytosine methylation in cpg doublets. in contrast, in phenotypically untransformed hybrids between these cells and uninfected rat or mouse cells, rsv inactivity is associated with hy ... | 1985 | 2990899 |
| enhancer activity correlates with the oncogenic potential of avian retroviruses. | avian retroviruses lacking an oncogene, such as rous-associated virus 1 (rav-1), rav-2, and td mutants of rous sarcoma virus (rsv), can nevertheless cause leukemias and other neoplastic diseases. during this process, viral dna integrates near a cellular proto-oncogene, such as c-myc, and thus de-regulates its expression. the virus rav-0, on the other hand, is known to be non-oncogenic even in long-term in vivo infections of domestic chickens. the major difference between oncogenic and non-oncoge ... | 1985 | 2990916 |
| partial nucleotide sequence of rous sarcoma virus-29 provides evidence that the original rous sarcoma virus was replication defective. | rous sarcoma virus-29 (rsv-29) is the strain of rsv that has the least number of passages beyond its isolation from chicken tumor no. 1 among all current strains of rsv. biological characterization indicated that it was replication defective. rna analysis of nonproducer clones of rsv-29-infected chicken embryonic fibroblasts showed the presence of a subgenomic message of 2.6 kilobases containing src and a genomic rna of 7.7 kilobases that contains gag, pol, and src, but not env. the src-containi ... | 1985 | 2991593 |
| neoplastic transformation of normal and carcinogen-induced preneoplastic syrian hamster embryo cells by the v-src oncogene. | the ability of cloned rous sarcoma virus (rsv) dna encoding the v-src oncogene to neoplastically transform normal, diploid syrian hamster embryo (she) cells was examined. transfection of rsv dna into early passage she cells resulted in a low but significant number of tumors when treated cells were injected into nude mice. tumors formed with a low frequency (two tumors out of ten sites injected) and only after a long latency period (14 weeks). in contrast to the normal she cells, several differen ... | 1985 | 2991747 |
| [brain tumor induced in dogs by intracerebral inoculation of sr-rsv induced cultured tumor cells--electron microscopic study]. | an experimental transplantable canine brain tumor model with the advantages of rapid tumor growth within 10 days and relative safety for the investigator is presently available. the tumor is produced by intracerebral inoculation of cultured cells derived from a canine brain tumor induced by the schmidt-ruppin strain of the rous-sarcoma virus (sr-rsv). it has potential use as a model in experiments designed to evaluate the effectiveness of chemotherapy and radiotherapy with serial computerized to ... | 1985 | 2991791 |
| quail neural crest cells transformed by rous sarcoma virus can be established into differentiating permanent cell cultures. | quail neural crest cells derived from the truncal neural primordium, infected in vitro by rous sarcoma virus (rsv) in january 1978, were induced to multiply and have been established into permanent cultures. these cultures contain cells that differentiate into melanocytes, neuron-like cells and flat cells. about 50% of these different cell types are tetanus-toxin positive. electrophysiological studies have shown that some cells can generate action potentials similar to those reported in quail ne ... | 1985 | 2992711 |
| glutamic acid decarboxylase activity is stimulated in quail retina neuronal cells transformed by rous sarcoma virus and is regulated by pp60v-src. | rous sarcoma virus (rsv) stimulates in quail embryo neuro-retina (nr) cultures the specific activity of glutamic acid decarboxylase (gad), the enzyme responsible for the synthesis of gamma-aminobutyric acid, a major inhibitory neurotransmitter in nr and in central nervous system. in quail embryo nr cultures transformed by ts ny-68, a thermodependent transformation-defective mutant of rsv, stimulation of gad activity is regulated by pp60v-src, the product of the src gene of rsv. fibroblasts and m ... | 1985 | 2992933 |
| site-specific increased phosphorylation of pp60v-src after treatment of rsv-transformed cells with a tumor promoter. | when vole cells that had been transformed by rous sarcoma virus were treated with the tumor-promoting phorbol ester 12-o-tetradecanoyl-13-acetate (tpa), specific phosphorylation of pp60v-src was increased. partial v8 protease mapping indicated that the increased phosphorylation occurred exclusively on serine residues located in the amino terminus of the molecule. treatment of cells with dimethyl sulfoxide or 4 alpha-phorbol-12,13-didecanoate did not elicit this response. two-dimensional tryptic ... | 1985 | 2994221 |
| estimates of heritability of response to rous sarcomas of chickens. | the stocks used for this investigation consisted of 1039 f3 generation progeny from the cross of two highly inbred lines and 355 and 462 offspring from subpopulations unh 105a and unh 105b, respectively, of a noninbred line of new hampshires. matings were such that b complex alleles were segregated in the three experimental populations with minor exceptions. each chicken was inoculated at 6 weeks of age with a subgroup of rous sarcoma virus (rsv). resulting tumors were subjectively scored on a s ... | 1985 | 2995957 |
| wounding and its role in rsv-mediated tumor formation. | tumors induced in chickens by rous sarcoma virus remain localized at the site of injection even though the animals become viremic. tumors have now been shown to be inducible at other sites if a wound is inflicted or if the tissue is injured by administration of tumor promoters. these findings indicate that local wounding plays a role in the spread of tumorigenicity of rous sarcoma virus. | 1985 | 2996144 |
| establishment of a brain-tumor model in adult monkeys. | a brain-tumor model in adult monkeys may be significant because of the biological similarity to humans as well as the feasibility for surgical manipulation and for sequential computerized tomography (ct) scanning. in the present study, brain tumors were successfully produced in japanese monkeys (macaca fuscata), each weighing 2 to 10.8 kg, with an average age of 5.1 years old. tumor cells were implanted by intracerebral inoculation of 4 x 10(7) chick embryo fibroblasts infected with the schmidt- ... | 1985 | 2997416 |
| the gag and pol genes of bovine leukemia virus: nucleotide sequence and analysis. | the dna sequence of the gag and pol regions of a provirus cloned from a bovine tumor is presented. in order to confirm these results the sequence of portions of a second clone, derived from a virus-producing cell line, was also determined. the gag gene was found to consist of 1179 nucleotides, which probably encode only three proteins: an n-terminal protein of 109 amino acids, a major core protein (p24) of 215 amino acids, and a nucleic acid binding protein (p12) of 69 residues. an open reading ... | 1985 | 2997990 |
| the mannosidase inhibitors 1-deoxymannojirimycin and swainsonine have no effect on the biosynthesis and infectivity of rous sarcoma virus. | the effects of inhibitors, which interfere with oligosaccharide trimming by blocking mannosidases, on the processing and export of the viral glycoproteins of rous sarcoma virus (rsv), have been studied. 1-deoxymannojirimycin (dim) prevents removal of mannose residues from the man9 (glcnac)2 oligosaccharide whereas swainsonine (sw) blocks at a later stage resulting in the formation of so-called hybrid oligosaccharides. under a regime of these inhibitors, proteolytic cleavage of the viral glycopro ... | 1985 | 2998010 |
| pp60src-dependent protein phosphorylation in membranes from rous sarcoma virus-transformed chicken embryo fibroblasts. | the rous sarcoma virus (rsv)-transforming protein, pp60src, is a plasma membrane-associated tyrosine-specific protein kinase. a 36,000-da cellular polypeptide (p36) which is phosphorylated at tyrosine in rsv-transformed chicken embryo fibroblasts (rsv-cef) is also plasma membrane associated. to determine if p36 is directly phosphorylation and kinase activity in situ in the plasma membrane, src-dependent protein phosphorylation in membranes isolated from rsv-cef has been characterized. these memb ... | 1985 | 2998019 |
| influence of base-pairing in the leader region on in vitro translation of rous sarcoma virus rna. | the capacity of the leader region of rous sarcoma virus (rsv) rna to act as a regulator of viral protein synthesis was tested in vitro. when dna/rna hybrids of sufficient length (greater than 90-100 nucleotides) are created within the leader, synthesis of pr76gag is inhibited. the inhibition is dependent upon the length of the hybrid rather than its position with the exception that encumberance of the 5'-terminal 33 nucleotides does block translation. these findings demonstrate that physical alt ... | 1985 | 2998116 |
| modulation of fibronectin gene activity in chick embryo fibroblasts transformed by a temperature-sensitive strain (ts68) of rous sarcoma virus. | transcriptional regulation of the fibronectin gene is a major mechanism for lowering steady-state levels of fibronectin mrna in chick embryo fibroblasts (cef) transformed by rous sarcoma virus (rsv) (1). in the present study, we have measured the change of transcriptional activity of the fibronectin gene in cef transformed by a temperature-sensitive strain of rsv (ts68). ts68-cef maintained at either 35 degrees c or 41 degrees c were shifted to 41 degrees c or 35 degrees c, respectively, at 5-ho ... | 1985 | 2999717 |
| response of b complex haplotypes b22, b24, and b26 to rous sarcomas. | five individual male matings of line unh 105 new hampshires, in which all males and most females were either b22/b24 or b22/b26, produced 462 progeny that fell into six b complex genotypes: b22/b22, b24/b24, b26/b26, b22/b24, b22/b26, and b24/b26. the genotypes of parents and offspring were determined by blood typing for b alloantigens using a panel of antisera. six-week-old chickens were inoculated with rous sarcoma virus (rsv). resulting tumors were scored for size six times over a 10-week per ... | 1985 | 2999742 |
| [changes of the expression of histocompatibility (hla) antigens in human fibroblasts transformed by the avian leukosis-sarcoma virus]. | monoclonal monomorphic antibodies anti-hla class i and ii antigens, were used to evaluate the expression of these molecules on normal and rsv-transformed human fibroblasts. the results indicate that the human diploid fibroblasts transformed in vitro by rsv present a reduced hla-class i antigens expression as compared to the uninfected fibroblasts of the same donor. in parallel, it is demonstrated that the class ii molecules absent on normal cells are expressed after transformation. | 1985 | 3000539 |
| seroepidemiologic survey for antibodies to selected viruses in the respiratory tract of lambs. | a serologic study was conducted to determine the prevalence of antibodies to, and infection rate of, mastadenovirus ovi 5, m ovi 6, parainfluenza-3 (pi-3) virus, bovine herpesvirus-1 (bhv-1), respiratory syncytial virus (rsv), bovine viral diarrhea (bvd) virus, and ovine progressive pneumonia (opp) virus in lambs at a ram lamb growth-rate test station. for 2 consecutive years, serum samples were prepared from blood collected from 1- to 2-month-old ram lambs as they entered the test station (1st ... | 1985 | 3002217 |
| characterization of a growth-inhibiting protein present in rat serum that exerts a differential effect on in vitro growth of nonmalignant rat liver cells when compared with rous sarcoma virus-transformed rat liver cells. | we have previously reported the transformation by rous sarcoma virus of a cloned epithelial cell line (brl) established from buffalo rat liver by h. coon. the nontransformed (brl) and transformed (rsv-brl) cells grew at comparable rates in culture, whereas only the transformed cells were tumorigenic in vivo. we report here on the existence in rat and mouse sera of a growth inhibitor for the nontransformed brl cells. the transformed brl cells (rsv-brl) were insensitive to this inhibitor. the inhi ... | 1985 | 3003021 |
| regulation of cellular morphology by the rous sarcoma virus src gene: analysis of fusiform mutants. | we have been interested in how rous sarcoma virus (rsv) influences transformed cell morphology and compared the molecular properties of chicken embryo cells (cec) infected with mutants of rsv that induce the fusiform transformed cell morphology with those of cec infected by wild-type rsv, which induces the more normal round transformed cell morphology. we looked for properties shared by all fusiform mutant-infected cells, because these may be responsible for maintaining the fusiform morphology. ... | 1985 | 3018500 |
| respiratory syncytial virus infection of human mononuclear leukocytes in vitro and in vivo. | recurrent infections with respiratory syncytial virus (rsv) have been well documented despite serological evidence of prior exposure of the host and the absence of clear evidence of antigenic variation of the virus. therefore, human mononuclear leukocytes, as well as purified lymphocytes and monocytes-macrophages, were exposed to rsv in vitro and examined for expression of viral antigens by using indirect immunofluorescence with monoclonal antibodies to rsv. rsv infected both human monocytes-mac ... | 1985 | 2931491 |
| [bronchiolitis. our clinical experience in the 4 years from 1981 to 1984]. | the clinical experience of 661 children with bronchiolitis is reported in four-years period to gain a better understanding of diagnosis and pathogenesis of bronchiolitis. upper airways infections, expiratory dyspnea, clear sound by chest percussion, vesicular rales and whistling by chest auscultation, air trapping on the chest radiography were considered as essential data of diagnosis. it was found in 595 patients: expiratory dyspnea, air trapping, vesicular r. and whistling in 85% and whistling ... | 1985 | 3837240 |
| respiratory syncytial virus and heart block--cause and effect? | a child aged 3 years who developed a respiratory syncytial virus infection is described. his admission was complicated by the development of an irregular pulse. he initially had a variable first or second degree heart block with transient electrocardiographic evidence suggestive of pericarditis and/or myocarditis and later developed complete heart block. he has remained asymptomatic for the past four years. a search for other causes of the heart block proved negative. it is tempting to suggest a ... | 1985 | 3859266 |
| seroprevalence of respiratory syncytial virus in free-ranging bighorn sheep. | sera from 187 (42%) of 447 free-ranging bighorn sheep (ovis canadensis) in 9 western states had antibody titers to respiratory syncytial virus (rsv). titers were from 1:5 to 1:640, as determined by the standard virus neutralization test. results of the survey indicated that rsv is widespread in free-ranging bighorn sheep populations and that rsv may be an important factor in the bighorn sheep pneumonia-complex. | 1985 | 3878355 |
| management of bronchiolitis. | the incidence, etiology, epidemiology, clinical presentation, treatment, prognosis, and prevention of bronchiolitis are discussed with a critical evaluation of the available studies on various therapeutic approaches. bronchiolitis is a lower respiratory-tract viral infection that affects 6-10% of all children below two years of age. respiratory syncytial virus (rsv) is the usual pathogen. the symptoms range from mild wheezing to severe respiratory distress. an infected child usually has a fever, ... | 1985 | 3891201 |
| properties of a respiratory syncytial virus isolated from a sheep with rhinitis. | a virus isolated from a yearling cross-bred ewe was identified as respiratory syncytial virus (rsv) by indirect immunofluorescence and by virus neutralization with bovine rsv antisera. the virus caused a mild conjunctivitis in 3-month-old lambs when inoculated alone. although clinical signs of pneumonia were not observed, there was gross and microscopic evidence of pulmonary inflammation in the lungs of lambs inoculated with either the sheep rsv isolate alone or in conjunction with pasteurella h ... | 1985 | 3893243 |
| quantification of respiratory syncytial virus polypeptides in nasal secretions by monoclonal antibodies. | an indirect enzyme-linked immunosorbent assay (elisa) which uses monoclonal antibody as solid-phase immunosorbent was developed to measure specific polypeptides of respiratory syncytial virus (rsv). the assay was used to examine 43 nasopharyngeal (np) aspirates from rsv-positive infants that had been examined previously for rsv by culture, direct immunofluorescence, and polyclonal antibody elisa. frozen np aspirates were serially diluted and examined for the 66k mol. wt. fusion glycoprotein (f), ... | 1985 | 3894575 |
| practical recommendations for the detection of pediatric respiratory syncytial virus infections. | in our private clinic-hospital setting, respiratory syncytial virus (rsv) was isolated from infants more frequently and sooner from nasal washes (84%; 4.2 days) than from throat swabs (45%; 5.5 days) or nasopharyngeal swabs (39%; 5.7 days). immunofluorescence of nasal wash cells identified 72% of the infants with virus isolations from nasal washes in less than one day. we therefore recommend the combination of isolation and immunofluorescence on nasal wash specimens for optimal detection of rsv- ... | 1985 | 3897268 |
| development of cell-mediated cytotoxic activity in the respiratory tract after experimental infection with respiratory syncytial virus. | the development of natural killer cell and other antibody-independent cellular cytotoxic response to rsv were studied in splenic and pulmonary mononuclear effector cells obtained from groups of 6-week-old cotton rats after subcutaneous (sc) or intranasal (in) immunization with live virulent or ultra-violet (uv) inactivated respiratory syncytial virus (rsv). no virus-induced cytotoxic activity was observed after sc immunization with live virus or in inoculation of inactivated non-infectious virus ... | 1985 | 3899431 |
| the production of monoclonal antibodies against respiratory syncytial virus and its clinical applications. | the availability of monoclonal antibodies against various viral components, either internal nucleoproteins or membrane proteins, has greatly advanced our understanding of the biochemistry of respiratory syncytial virus (rsv). the components responsible for serum neutralization have been identified by monoclonal antibodies, thus facilitating the development of synthetic rsv vaccine. a number of clinical tests have been devised using monoclonal antibodies for either qualitative or quantitative det ... | 1985 | 3899483 |
| immunoprophylaxis and immunotherapy of respiratory syncytial virus infection in the cotton rat. | human convalescent antiserum to respiratory syncytial virus (rsv) administered intraperitoneally to cotton rats prior to rsv challenge provided near-complete protection from pulmonary infection. antiserum given subsequent to viral challenge reduced pulmonary viral titers 100-fold or greater within 24 h. sandoglobulin, a preparation of purified human igg with high titer of anti-rsv neutralizing activity, produced the same effects as convalescent antiserum. sandoglobulin was absorbed rapidly and p ... | 1985 | 3907188 |
| calf pneumonia. | infectious calf pneumonia is a high-mortality pneumonia of housed dairy-type calves. viruses are important etiologic agents and infection with bovine respiratory syncytial virus (rsv) and parainfluenza type 3 virus (pi-3 virus) may result in extensive, and sometimes fatal, lung damage. respiratory viral infections are frequently followed by mycoplasmal and secondary bacterial invasion of the lower respiratory tract, which increases the extent and severity of lung damage. bad housing, particularl ... | 1985 | 3907774 |
| respiratory syncytial virus infection in immunosuppressed animals: implications in human infection. | neonatal cotton rats were treated with cyclophosphamide parenterally for three weeks before intranasal inoculation of live respiratory syncytial virus (rsv). immunosuppressive therapy resulted in severe depletion of lymphocytes from the peripheral circulation, the spleen, and the thymus. in contrast to normal rats, immunosuppressed animals developed severe pulmonary pathology with marked infiltration of foamy macrophages. persistent degeneration and regeneration of bronchial epithelial cells wer ... | 1985 | 3908616 |
| immunoprecipitation of insulin receptors from cultured human lymphocytes (im-9 cells) by antibodies to pp60src. | the family of tyrosine-specific protein kinases includes proteins encoded by retroviral oncogenes as well as receptors for insulin and several growth factors. antibodies to pp60src, the protein encoded by the src oncogene of rous sarcoma virus (rsv), can specifically immunoprecipitate affinity-labeled insulin receptors from cultured human lymphocytes (im-9 cells). this precipitation is specifically inhibited by the src gene product purified from rsv-transformed rat cells. these observations prov ... | 1985 | 3918346 |
| an n-terminal peptide from p60src can direct myristylation and plasma membrane localization when fused to heterologous proteins. | the src gene product, p60src, of rous sarcoma virus (rsv) is a tyrosine-specific protein kinase which is associated with the plasma membrane of infected cells. myristic acid is bound in an amide linkage to glycine 2 of p60src. of the n-terminal 30 kilodaltons of p60src, only amino acids 1-14 are required for myristylation, and myristylation of p60src may be required for its membrane association, and for cell transformation. to test the hypothesis that the first 14 amino acids of p60src contain a ... | 1985 | 3920530 |
| close structural resemblance between putative polymerase of a drosophila transposable genetic element 17.6 and pol gene product of moloney murine leukaemia virus. | we have made a computer-assisted search for homology among polymerases or putative polymerases of various viruses and a transposable element, the drosophila copia-like element 17.6. the search revealed that the putative polymerase (second open reading frame) of the copia-like element 17.6 bears close resemblance in overall structural organization to the pol gene product of moloney murine leukaemia virus (m-mulv): they show significant homology to each other at both the n- and c-terminal portions ... | 1985 | 2408886 |
| inhibition of rous sarcoma virus production by formycin. | the effect of formycin, an adenosine analog, on the growth of chick embryo fibroblasts and on rous sarcoma virus (rsv) production was studied. an adverse effect on cell proliferation was observed in the presence of 10 microm formycin. treatment with 5 microm formycin for 24 hr reduced by a factor of about 1000 the yield of infections progeny whereas the cell growth remained unaltered. moreover the few particles released in the presence of formycin showed a markedly decreased ability to synthesiz ... | 1985 | 2409669 |
| rous sarcoma virus-transformed fibroblasts adhere primarily at discrete protrusions of the ventral membrane called podosomes. | rous sarcoma virus-transformed bhk cells (rsv/b4-bhk) adhere to a fibronectin-coated substratum primarily at specific dot-shaped sites. such sites contain actin and vinculin and represent close contacts with the substratum as revealed by interference reflection microscopy. only a few adhesion plaques and actin filament bundles can be detected in these cells as compared to untransformed parental fibroblasts. in thin sections examined with transmission electron microscopy (tem) these adhesion site ... | 1985 | 2411576 |
| cytotoxic t-cell response to respiratory syncytial virus in mice. | the role of the humoral and cellular arms of the immune response in protection against respiratory syncytial virus (rsv) infection and in the pathogenesis of the severe forms of this disease is poorly understood. the recent demonstration that some inbred mouse strains can be infected with rsv has opened the way to a detailed investigation of rsv immunity. we report here the finding of major histocompatibility complex-restricted, rsv-specific memory cytotoxic t cells in the spleens of balb/c and ... | 1985 | 2411951 |
| neurones express high levels of a structurally modified, activated form of pp60c-src. | neural tissues contain high levels of the cellular homologue of the transforming protein of rous sarcoma virus (rsv), but neither the specific cell types expressing high levels of c-src, nor the function of the cellular src (c-src) protein has been determined. using primary culture methods, we have found that pure neurones and astrocytes derived from the rat central nervous system (cns) contain 15- to 20-times higher levels of the c-src protein than fibroblasts. however, the specific activity of ... | 1985 | 2412121 |
| vinculin and 36 kda protein are not tyrosine-phosphorylated in rous sarcoma virus infected cells which have been treated with interferon. | the expression of membrane-associated transformation-specific parameters was analyzed in de novo rous sarcoma virus (strain sr-rsv-d) infected chicken embryo fibroblasts pretreated with homologous interferon. cellular morphology, hexose transport, microfilament organization, and tyrosine-phosphate content of two primary substrates of the transformation-generating viral kinase, pp60src, were found indistinguishable from non-infected controls. these observations support the hypothesis that vinculi ... | 1985 | 2412817 |
| respiratory syncytial virus infection in transported calves. | nasal swab samples were collected from calves on individual farms in tennessee and sequentially at an auction barn and at a feedlot to detect respiratory syncytial virus (rsv). in 1976, rsv was isolated from 5 of 225 calves at the auction barn and from 13 of 92 calves examined at the feedlot. of the 13 isolations, 11 were from calves with acute respiratory tract disease. most (14/18) calves infected with rsv were also shedding parainfluenza-3 virus in their nasal secretions. attempts to isolate ... | 1985 | 2417510 |
| clinical applications of antiviral agents for chemophrophylaxis and therapy of respiratory viral infections. | table iii summarizes clinical applications of antiviral agents in respiratory viral infections. (table: see text) for influenza a virus infections, both oral amantadine and rimantadine are effective when used for seasonal prophylaxis and for prophylaxis in institutional populations. both of these drugs, as well as aerosolized ribavirin, have antiviral and therapeutic effects in uncomplicated influenza. it remains to be determined whether any of these modalities or possibly their combined use [44 ... | 1985 | 2417551 |
| gene expression from both intronless and intron-containing rous sarcoma virus clones is specifically inhibited by anti-sense rna. | to distinguish the inhibitory effect of anti-sense rna on translation from the effect on splicing, a plasmid (plc32) was constructed from a cdna clone of the rous sarcoma virus (rsv) envelope gene (env) mrna. transcription of this plasmid results in the synthesis of rna identical to the rsv env gene mrna which does not require splicing to be expressed. plasmids derived from plc32 were also constructed in which the env gene coding sequence and 5' noncoding leader sequences were inserted in the op ... | 1985 | 2426579 |
| enhancement of immunity against rsv-induced sarcomas by generation of hapten-reactive helper t lymphocytes. | previous work from this laboratory has shown that preimmunization of syngeneic hosts with rous sarcoma virus (rsv)-transformed cells elicits a strong immune response against the growth of transplantable rsv sarcomas, mediated by t lymphocytes expressing the surface phenotype of helper cell precursors (prat, di renzo & comoglio, 1983). this paper shows that anti-tumour immunity may be elicited in tumour-bearing animals by triggering an experimentally pre-amplified t-helper cell population at the ... | 1985 | 2578431 |
| antigenic characterization of respiratory syncytial virus strains with monoclonal antibodies. | to study the antigenic characteristics of respiratory syncytial virus (rsv), we developed and evaluated monoclonal antibodies (mabs) to three strains of rsv: 11 to long, 4 to 18537, and 9 to a2. six of these mabs immunoprecipitated the nucleoprotein, six the large glycoprotein, and 11 the fusion protein. by the pattern of the reactions of these mabs to 16 strains of rsv in an indirect immunofluorescence assay or enzyme-linked immunosorbent assay, we were able to distinguish three subgroups. with ... | 1985 | 2579169 |
| antigenic analysis of a putative new strain of respiratory syncytial virus. | a recent isolate (9320) of respiratory syncytial virus (rsv) that had been reported to differ from all previous strains was compared with rsv strains isolated over a period of 23 years by using the techniques of in vitro neutralization by specific antiserum and in vivo resistance to infection in cotton rats. the 9320 strain did not appear to be a novel antigenic variant and exhibited greater homology to several prototype strains than did an earlier isolate. the antigenic differences detected by ... | 1985 | 2579170 |
| human and murine cytotoxic t cells specific to respiratory syncytial virus recognize the viral nucleoprotein (n), but not the major glycoprotein (g), expressed by vaccinia virus recombinants. | the viral antigens recognized by cytotoxic t cells (ctl) have not been defined in most viruses infecting mouse or man. natural or artificial virus recombinants can be used to determine the antigen specificity of ctl directed against viruses with segmented genomes, such as influenza, but this technique is more difficult to apply to the study of unsegmented viruses. we describe here the use of recombinant vaccinia viruses, containing cdna corresponding to either the nucleoprotein (n) gene or the m ... | 1986 | 2431051 |
| concurrent circulation of antigenically distinct strains of respiratory syncytial virus during community outbreaks. | respiratory syncytial virus (rsv) is considered to be of a single serotype. antigenic variants are detectable both by neutralization and monoclonal antibodies and have been divided into two broad categories, groups 1 and 2. group 2 isolates have been considered to be uncommon. we used indirect immunofluorescence with strain-specific monoclonal antibodies to study rsv isolates from hospitalized infants in the greater boston area. of 223 rsv isolates recovered over a five-month period in 1983-1984 ... | 1986 | 2418126 |
| the detection of respiratory syncytial virus in nasopharyngeal aspirates: assessment, formulation, and evaluation of monoclonal antibodies as a diagnostic reagent. | comparisons were made between standard methods of cell culture, indirect immunofluorescence (if) using hyperimmune respiratory syncytial virus (rsv) antiserum, and indirect if using mouse monoclonal antibodies directed against various epitopes of rsv for the detection of rsv in nasopharyngeal aspirates. the monoclonal antibodies were used singly and in pools of different specificities which in turn were tested in both direct and indirect if. in a preliminary study, aspirates from 227 infants wer ... | 1986 | 2419492 |
| enhancement of respiratory syncytial virus pulmonary pathology in cotton rats by prior intramuscular inoculation of formalin-inactiva ted virus. | cotton rats previously inoculated with formalin-inactivated respiratory syncytial virus (rsv) were challenged intranasally with live rsv to induce an enhancement of rsv disease similar to that observed after the administration of formalin-inactivated rsv vaccine to human infants 20 years ago. within 24 h after infection with rsv, cotton rats developed pulmonary lesions that reached a maximum by day 4. histologically, the lesions resembled an experimental pulmonary arthus reaction. an action of f ... | 1986 | 2419587 |
| identification of epitopes on respiratory syncytial virus proteins by competitive binding immunoassay. | to characterize the interrelationship of monoclonal antibodies (mabs) against respiratory syncytial virus (rsv) and their respective epitopes, we developed a competitive binding assay based on the biotin-avidin system and a tissue culture enzyme-linked immunosorbent assay. the competitive binding assay clearly distinguished between competing and noncompeting mabs. eight mabs against the fusion protein (f protein) demonstrated two blocking patterns consistent with two antigenic sites. mabs reacti ... | 1986 | 2420819 |
| murine cytotoxic t cells specific to respiratory syncytial virus recognize different antigenic subtypes of the virus. | the aim of this study was to establish whether cytotoxic t cells (tc), raised against respiratory syncytial virus (rsv) in the mouse, are specific to the strain of immunizing virus, or cross-reactive between virus strains. several recent studies using monoclonal antibodies have begun to define the antigenic variation among strains of rsv. it is likely that a successful rsv vaccine will need to contain antigenic determinants from more than one subtype, but since only the highest levels of neutral ... | 1986 | 2420922 |
| polymerase-defective mutant of the bryan high-titer strain of rous sarcoma virus. | a mutant of the bryan high-titer strain of rous sarcoma virus defective in reverse transcriptase is known as type alpha (bh-rsv alpha). bh-rsv alpha virion particles do not contain any polymerase-related proteins but they direct the synthesis of a normal sized pr180 gag-pol polyprotein precursor in infected cells. using a bioassay for polymerase gene function that is based on the requirement of viral replication for transformation of transfected chicken cells, we have localized the defect to the ... | 1986 | 2421248 |
| phosphotyrosine-containing 120,000-dalton protein coimmunoprecipitated with pp60v-src from rous sarcoma virus-transformed mammalian cells. | serum from rabbits bearing tumors (tbr serum) induced by the rous sarcoma virus (rsv) was originally developed to identify the rsv src gene protein-pp60v-src. it is also capable of directly or indirectly immunoprecipitating a number of proteins besides pp60v-src from lysates of rsv-transformed cells. this report describes a highly phosphorylated protein of approximately 120,000 da (pp120) which is specifically immunoprecipitated from rsv-transformed mammalian cells by tbr sera and monospecific a ... | 1986 | 2421483 |
| phosphorylation of tyrosine residues of calmodulin in rous sarcoma virus-transformed cells. | calmodulin, a wide-spread eukaryotic ca2+-binding protein, was phosphorylated at its tyrosine residues in rous sarcoma virus (rsv)-transformed chicken and rat cells but not in normal chicken embryo fibroblasts. in contrast, serine and threonine phosphorylation of calmodulin was found to occur in both normal and virus-transformed cells. in an in vitro system containing purified src kinase from rsv-transformed cells, tyrosine phosphorylation of calmodulin by the src kinase was inhibited by ca2+. f ... | 1986 | 2424020 |
| radiological findings in children with respiratory syncytial virus infection: relationship to clinical and bacteriological findings. | respiratory syncytial virus (rsv) is a frequent cause of bronchiolitis leading to acute admission to hospital in the winter months. a wide range of findings accompanies this disease and the appearances are seldom completely diagnostic. associated bacterial co-infections are common and we have shown an association with atelectasis among patients with pathogenic bacteria in the nasopharynx. | 1986 | 3951895 |
| complement activation by respiratory syncytial virus-infected cells. | respiratory syncytial virus (rsv), a major respiratory pathogen of children, has been speculated to cause disease by immunologic mechanisms. although circulating levels of complement (c) are normal during rsv infections, the role of c in respiratory tract secretion is unclear. since epithelial cells of the respiratory tract of children infected with rsv express viral surface antigens, the ability of rsv infected human cells to activate c was studied. rsv infected human cells (hela) were found to ... | 1986 | 3954599 |
| comparison of washed nasopharyngeal cells and whole nasal secretions for detection of respiratory syncytial virus antigens by enzyme-linked immunosorbent assay. | we compared washed nasal epithelial cells with unfractionated nasal secretions as sources of respiratory syncytial virus (rsv) antigens in an indirect enzyme-linked immunosorbent assay (elisa). of 28 infants positive for rsv by virus isolation or direct immunofluorescence or both, 27 (96%) were positive by elisa with whole nasal secretions, whereas only 19 (68%) were positive by elisa with the matching washed-cell fractions. furthermore, the elisa absorbances obtained with nasal secretions were ... | 1986 | 3700621 |
| genetic aspects of antibody responses in chickens to different classes of antigens. | six breeding groups of chickens, each characterized by a different haplotype of the b blood group system, were challenged with different classes of antigens, namely newcastle disease vaccine (nd), infectious bronchitis vaccine (ib), infectious bursal disease viral agent (ibd), salmonella pullorum antigen (p), and sheep red blood cells (srbc). parents were challenged at 20 weeks of age, and their offspring were challenged at 3 weeks of age. blood samples were taken from the parents at 1 week afte ... | 1986 | 3703775 |
| [respiratory syncytial virus infections in newborn infants]. | twenty-three of 23 neonates were contaminated in the course of an outbreak of respiratory syncytial virus (rsv) in a neonatal care unit. symptoms among 22 infected symptomatic infants included rhinitis (n = 21), dyspnea (n = 19), cough (n = 17), apnea (n = 5), seizures (n = 3), fever (n = 3). five patients presented with severe respiratory distress. the occurrence of non-obstructive apnea was significantly correlated with a history of respiratory disease, rsv infection during the first 15 days o ... | 1986 | 3718165 |
| culture of respiratory syncytial virus infected diploid bovine nasal mucosa cells on cytodex 3 microcarriers. | in preliminary studies, a system for the propagation of respiratory syncytial virus (rsv) using cytodex 3 microcarriers was established. optimal growth conditions were defined in culture to be a microcarrier concentration of 1.5 g/l with a cell inoculum density of 4 x 10(4) cells/ml tissue culture medium. under these conditions growth coefficients were 3-fold greater in microcarriers when compared to conventional monolayer culture in roux culture flasks. maximum yield of virus antigen was achiev ... | 1986 | 3718237 |
| respiratory syncytial viral infection in children with compromised immune function. | for 10 winters, 608 children five years old or younger who were hospitalized with respiratory syncytial virus (rsv) infection were prospectively studied to evaluate the relation between their immune status and the severity of their infection. forty-seven had been immunocompromised by chemotherapy, steroid therapy, or a primary immunodeficiency disorder. among the immunocompromised children, those receiving chemotherapy for cancer and those with immunodeficiency disease had more severe rsv diseas ... | 1986 | 3724802 |
| respiratory syncytial virus: a report of a 5-year study at a children's hospital. | a study over a 5-year period (1979-1983) of rsv infections in children in sydney, australia is reported. in common with findings made elsewhere in the world, annual epidemics of rsv infection commencing in autumn and lasting 4-6 months, with peak activity in mid-winter, were observed in 1979, 1980, and 1983. however, in 1981 and 1982 virus activity was first detected in midsummer, peaked in autumn, and was present throughout most months of the year. the alteration in virus activity in 1981 and 1 ... | 1986 | 3746259 |
| serum and nasal-wash immunoglobulin g and a antibody response of infants and children to respiratory syncytial virus f and g glycoproteins following primary infection. | an enzyme-linked immunosorbent assay (elisa) with immunoaffinity-purified fusion (f) or attachment (g) glycoprotein was used to measure the serum and secretory immune responses of 18 infants and children, 4 to 21 months of age, who underwent primary infection with respiratory syncytial virus (rsv). most of the 10 older individuals (9 to 21 months of age) developed moderate levels of serum and nasal-wash immunoglobin a (iga) and igg f and g antibodies. these individuals developed a moderate level ... | 1986 | 3754878 |
| dissociation between serum neutralizing and glycoprotein antibody responses of infants and children who received inactivated respiratory syncytial virus vaccine. | the serum antibody response of infants and children immunized with formalin-inactivated respiratory syncytial virus (rsv) vaccine 20 years ago was determined by using an enzyme-linked immunosorbent assay specific for the rsv fusion (f) and large (g) glycoproteins and a neutralization assay. twenty-one young infants (2 to 6 months of age) developed a high titer of antibodies to the f glycoprotein but had a poor response to the g glycoprotein. fifteen older individuals (7 to 40 months of age) deve ... | 1986 | 3755730 |
| simplified enzyme-linked immunosorbent assay for specific antibodies to respiratory syncytial virus. | a simplified and reliable enzyme-linked immunosorbent assay (elisa) was applied to the detection of serum antibodies against respiratory syncytial virus (rsv). rsv-infected cells were fixed and dried on 96-well microtiter plates and kept at 4 degrees c. the titers of reference sera were determined by endpoint dilution. a linear relation was found between the titers and the logarithm of absorbance values of sera diluted to 1:1,000 (r = 0.93, p less than 0.001). measurement of rsv antibodies was d ... | 1986 | 3771748 |
| effect of age and preexisting antibody on serum antibody response of infants and children to the f and g glycoproteins during respiratory syncytial virus infection. | the serum antibody response of 50 infants and children infected with respiratory syncytial virus (rsv) was determined by a glycoprotein-specific enzyme-linked immunosorbent assay, and the effects of age and preexisting antibody titer at the time of rsv infection on response to the g and f glycoproteins of rsv were examined. the immune response to the g and f glycoproteins was assessed with anti-human immunoglobulin a to permit measurement of the response of young infants in the presence of mater ... | 1986 | 3771779 |
| predictive value of respiratory syncytial virus-specific ige responses for recurrent wheezing following bronchiolitis. | to determine whether the magnitude of the respiratory syncytial virus (rsv)-specific ige response at the time of an episode of rsv bronchiolitis in infancy accurately predicts the development of subsequent wheezing episodes, we observed 38 infants prospectively from the time of an episode of infantile bronchiolitis through 48 months of age. peak rsv-ige titers were measured at the time of the bronchiolitis episode using an elisa procedure. notation was made of both the number of subsequent wheez ... | 1986 | 3772657 |
| gianotti-crosti syndrome associated with infections other than hepatitis b. | although the gianotti-crosti syndrome (gcs) is regularly associated with hepatitis b infection elsewhere, in north america that association is rarely made. accordingly, we studied nine children with acral, symmetrical eruptions typical of gcs for evidence of other infections. all were negative for hepatitis b surface antigen. viral cultures were done in nine patients, and viruses isolated in two. one patient with a respiratory prodrome had respiratory syncytial virus (rsv) isolated, and a second ... | 1986 | 3773143 |
| the use of eye-nose goggles to control nosocomial respiratory syncytial virus infection. | we evaluated an eye-nose goggle to determine its usefulness in reducing nosocomial rsv infection in patients and staff members on our infant ward. during a community outbreak of rsv in 1984, infection was assessed by biweekly routine viral cultures on all ward personnel and patients and also by seroconversion in personnel. for three weeks staff members wore the goggles; two (5%) adults and one (6%) child acquired nosocomial infection. during the subsequent three-week study period, goggles were n ... | 1986 | 3773177 |
| age related igg subclass response to respiratory syncytial virus fusion protein in infected infants. | respiratory syncytial virus (rsv) fusion protein was purified by immunoaffinity chromatography using a mouse monoclonal antibody coupled to affi-gel 10. the fusion protein was homogeneous by sodium dodecyl sulfate polyacrylamide gel electrophoresis (sds-page) and free of other detectable viral or cellular protein. the purified fusion protein was used in a quantitative enzyme-linked immunosorbent assay (elisa) to measure the age-related antibody response to this protein in infected infants. the f ... | 1986 | 3791688 |
| immunological similarity between the insulin receptor and the protein encoded by the src oncogene. | insulin receptors resemble receptors for certain growth factors (epidermal growth factor, platelet-derived growth factor, and insulin-like growth factor i) in that all possess tyrosine-specific protein kinase activity. these cell surface receptors resemble protein kinases encoded by viral oncogenes in that both groups of enzymes phosphorylate proteins on tyrosine. recently, we reported that there is immunological similarity between the insulin receptor and pp60src [the protein encoded by the src ... | 1986 | 3084215 |
| functional analysis of the human tissue-type plasminogen activator protein: the light chain. | a pbr322::rous sarcoma virus(rsv)-based shuttle vector was used to insert fused genes, composed of the amino-terminal portion of the bacterial chloramphenicol-acetyltransferase gene (cat) and the entire coding region for the c-terminally derived light (l) chain of human tissue-type plasminogen activator (t-pa) cdna. cotransfection of rat 3y1 cells with prsvneo dna and prsvcat/t-pa dna yielded stably integrated g418-resistant transfectants which contain unrearranged copies of prsvcat/t-pa dna. th ... | 1986 | 3087818 |
| [rhinovirus and acute respiratory infections in infants]. | human leucocytary interferon (ifn) in a concentration of 2,000 ul/ml stops the development of 9 rhinovirus (rv) strains. the use of serum ifn antibodies (saif) reverses this effect. of 256 nasal aspirates culture negative for other respiratory viruses, 40 rv were isolated with saif as opposed to 19 without serum anti ifn. the frequency and some clinical aspects are emphasized in infants: 43% had bronchiolitis similar to respiratory syncytial virus (rsv) infections and atopy was frequent. | 1986 | 3028312 |
| differential transient and long-term expression of dna sequences introduced into t-lymphocyte lines. | we have used a protoplast fusion protocol to introduce the genes encoding neomycin phosphotransferase (neo) and chloramphenicol acetyltransferase (cat) into murine and human t-lymphocyte lines. plasmid constructs containing the neo gene under the control of the promoters from the rous sarcoma virus long terminal repeat (rsv ltr), the sv40 early region, or the herpes simplex virus thymidine kinase gene (hsv tk) can stably transform each of three t-cell lines to g-418 resistance. the characteristi ... | 1986 | 3028736 |