Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| heteroduplex analysis of cloned rat endogenous replication-defective (30 s) retrovirus and harvey murine sarcoma virus. | 1980 | 6255681 | |
| moloney murine sarcoma proviral dna is a transcriptional unit. | a portion of moloney murine sarcoma virus dna which is repeated at both ends of the provirus has been sequences. the nucleotide sequence, together with hybridization data obtained with in vitro pulse-labelled nascent viral rna, indicate that initiation and termination of rna synthesis occur within that region of the proviral dna. a model for transcriptional readthrough of termination signals during rna synthesis in this system is suggested. | 1980 | 6256637 |
| defective cytotoxic t-cell generation in moloney murine sarcoma virus-infected a/sn mice. | cytotoxic t-cells (ctl) could not be detected in spleen cell suspensions from moloney murine sarcoma virus (m-musv)-induced tumor-bearing a/sn and (a/sn x c57bl/6) f1 mice, with the a/sn-derived natural killer (nk)-sensitive yac-1 lymphoma cells used as targets. however, spleen t-cells from tumor-bearing (a/sn x c57bl/6)f1 mice were efficient killers against c57bl/6-derived rbl-5 cells. when tested for viral antigens by sera from mice with regressing atumors, yac-1 and rbl-5 cells cross-reacted. ... | 1980 | 6968372 |
| guanine nucleotide binding activity of the src gene product of rat-derived murine sarcoma viruses. | 1980 | 6261655 | |
| increased sensitivity of fibroblasts of skin from patients with adenomatosis coli and peutz-jegher's syndrome to transformation by murine sarcoma virus. | skin fibroblasts from family members of cases of adenomatosis coli (ac) and peutz-jegher's syndrome (pj) were examined for susceptibility to morphological transformation by moloney murine sarcoma virus (msv) of rate 78ai cells, which was found in the present study to contain both dualtropic and ecotropic murine leukemia virus (mlv). the fibroblasts from individuals with manifest ac (22 subjects) and manifest pj (5 subjects) were up to 5 times more susceptible to transformation by rat 78ai-msv(ml ... | 1980 | 6268477 |
| structure of and alterations to defective murine sarcoma virus particles lacking envelope proteins and core polyprotein cleavage. | htg2 hamster cells produce a defective murine sarcoma virus lacking gp70 and, consequently, viral surface projections (knobs), but the lack of knobs appears to have no effect on intramembrane particle distribution. in addition, it has been noted that the core of the virus remains in the "immature" form as a result of the failure of the polyprotein precursor (p65) to undergo cleavage. however, incubation of htg2 virus with avian myoblastosis virus was found to yield specific cleavage products of ... | 1980 | 6257930 |
| [a virus produced by human embryonic cells infected with moloney murine sarcoma virus]. | human embryonic cell cultures, infected with the mouse sarcoma virus, complex (moloney isolate), produced a virus that specifically transformed mink lung cells. the non-transforming helper virus could infect mouse cells as well as a wide range of other mammalian cells. these observations could suggest the presence of either several viruses in the virus stock and/or stable recombinants. | 1980 | 6774828 |
| the biology, pathology and immunology of a virus induced rat osteosarcoma. i. biological behaviour and histopathology. | the biological behaviour and histopathology of an experimental osteosarcoma in rats are described as a model to study the human disease. the tumour was developed by injection of moloney murine sarcoma virus into the tibial marrow space of three strains of inbred new-born rats. the resulting neoplasm was highly malignant and arose after a short latent period of only 10 days. it was readily maintained in tissue culture and was transplantable to adult rats. the virus induced tumour resembled human ... | 1980 | 6937449 |
| antitumor effects of doxorubicin against a virally-induced rat osteosarcoma with minimal immunosuppression. | intratibial inoculation of a moloney strain of murine sarcoma virus (msv-m) in neonatal wistar-lewis rats produced osteosarcoma in 96% of animals and resulted in a median survival of 20 days. intraperitoneal (i.p.) administration of doxorubicin (adriamycin) (1-2 mg/kg/d, on day 10-12) resulted in reduced tumor growth and prolonged median survival to 95+ and 64 days, respectively. higher dose doxorubicin (3-4 mg/kg/d, on day 10-12) caused early lethal toxicity. autopsy data revealed a characteris ... | 1980 | 6937562 |
| experimental osteosarcoma in the mouse, induced by moloney's murine sarcoma virus. | the writers report an experimental trial on the mouse, consisting in the induction of osteosarcoma by inoculation of moloney's murine sarcoma virus (msv-m) into the proximal metaphysis of the tibia. this experiment was conducted on 90 balb/c female mice aged twenty-one days and of mean weight 12.8 g. one group of animals was also given immunodepressive drugs. the tumour developed in 100 per cent of cases, producing metastases in some of the animals and resulting in death in three cases. the radi ... | 1980 | 6938501 |
| polyclonal antibody synthesis and autoantibody formation in mice inoculated with murine sarcoma virus. | 1980 | 6989530 | |
| effect of lactate dehydrogenase virus on chemically induced mouse lung tumorigenesis. | lactate dehydrogenase virus is the third in a series of viruses which have been examined for the capacity to alter chemically induced mouse lung tumorigenesis. this virus was given to strain a mice by i.p. injection either 4 weeks before, on the same days as, 4 weeks after, or 8 weeks after the s.c. injection of urethan (either 0.25 or 1.0 mg/g). the pulmonary adenoma response to urethan was suppressed in all of the lactate dehydrogenase-infected mice, with a maximum suppression of 30 to 40% whe ... | 1980 | 7349905 |
| intracellular rna complementary to the rna genome of the moloney--murine sarcoma virus complex. | 1980 | 7352370 | |
| in vitro translation of virion rna from moloney murine sarcoma virus. | 1980 | 7355580 | |
| effect of canavanine on murine retrovirus polypeptide formation. | canavanine is an arginine analog which is widely used to inhibit proteolytic processing of viral polyproteins. certain results obtained with canavanine have suggested that it may have other effects. therefore, we examined the effects of canavanine on the cell-free synthesis of murine retrovirus proteins. it was found that the electrophoretic mobility of the major gag-related cell-free product of both rauscher murine leukemia virus (r-mulv) and moloney murine sarcoma virus 124 (mo-musv-124) rna w ... | 1980 | 7365878 |
| suppression of murine retrovirus polypeptide termination: effect of amber suppressor trna on the cell-free translation of rauscher murine leukemia virus, moloney murine leukemia virus, and moloney murine sarcoma virus 124 rna. | the effect of suppressor trna's on the cell-free translation of several leukemia and sarcoma virus rnas was examined. yeast amber suppressor trna (amber trna) enhanced the synthesis of the rauscher murine leukemia virus and clone 1 moloney murine leukemia virus pr200(gag-pol) polypeptides by 10- to 45-fold, but at the same time depressed the synthesis of rauscher murine leukemia virus pr65(gag) and moloney murine leukemia virus pr63(gag). under suppressor-minus conditions, moloney murine leukemi ... | 1980 | 7373716 |
| effects of sodium butyrate on the membrane glycoconjugates of murine sarcoma virus-transformed rat cells. | the temporal relationship between butyrate-induced cellular flattening of murine sarcoma virus-transformed rat cells (msv-nrk) and alterations in certain surface-associated biochemical markers of transformation, e.g., surface glycopeptides, glycolipids, fibronectin, hexose uptake, and cell-substrate adhesion was examined. the induction of elevated levels of the ganglioside gm3 and of a gdla-like ganglioside were observed to precede or to parallel cellular flattening. likewise, enhanced incorpora ... | 1980 | 7380882 |
| genome organization of retroviruses. viii. nonproducer cell lines of mouse fibroblasts transformed by moloney murine sarcoma virus dna synthesized in vitro. | 1980 | 7395109 | |
| cytochalasin b-induced multinucleation of human tumor and normal cell cultures. | twelve human cell cultures derived from tumors, normal tissues, and derivative cultures transformed by either a rna tumor virus or chemical carcinogen were examined for their response to cytochalasin b (cb) and the expression of this marker was correlated with growth in soft agar and saturation density in monolayer culture. cell lines derived from carcinoma of the bladder (t24 and rt4), kidney (caki-1), prostate (du 145), and breast (mcf-7) multinucleated when growth in cb-supplemented medium, w ... | 1980 | 7397790 |
| isolation and characterization of naturally occuring deletion mutants of moloney murine sarcoma virus. | 1980 | 7423856 | |
| partial characterization of a moloney murine sarcoma virus 85,000-dalton polypeptide whose expression correlates with the transformed phenotype in cells infected with a temperature-sensitive mutant virus. | 1980 | 7423857 | |
| murine sarcoma growth factors affect the growth and morphology of cultured mouse epithelial cells. | a mouse embryo epithelial cell line, mmc-e, was used to study the effects of purified murine sarcoma growth factors (sgfs) on the growth of epithelial cells. murine sgf was a partially purified preparation from the serum-free culture media of mouse fibroblasts transformed by moloney murine sarcoma virus. sgfs stimulated dna-synthesis in resting cells and induced them to grow to higher densities than control cells. with continued exposure to sgfs, mmc-e cells lost their postconfluency inhibition ... | 1980 | 7440645 |
| in vivo antigenic modification of tumor cells. i. introduction of murine leukemia virus antigens on non-virus-producing murine sarcomas. | murine oncovirus antigens represent excellent targets for immune recognition, and virus-associated tumors are generally susceptible to various immunotherapy protocols. virus-negative tumors, however, are nonimmunogenic and refractory to immunologic control. therefore, the feasibility of the introduction of antigens onto non-virus-expressing tumors in situ in inbred c57bl/6j mice by systemic administration of nononcogenic murine retroviruses was investigated. two classes of murine fibrosarcomas w ... | 1981 | 6942181 |
| in vivo antigenic modification of tumor cells. ii. distribution of virus in sarcoma-bearing mice. | murine leukemia viruses were previously demonstrated to be able to infect efficiently non-virus-expressing tumors in vivo. in the present study the infectivity and tissue distribution of friend murine leukemia virus (f-mulv) in normal and tumor-bearing c57bl/6j (b6) mice were examined. two syngeneic fibrosarcoma-inducing cell lines were used: cells from a 3-methylcholanthrene-induced fibrosarcoma syngeneic to b6 mice (mca-fs) and cells from a harvey murine sarcoma virus-transformed, nonproducer ... | 1981 | 6942182 |
| ldhk, an unusual oxygen-sensitive lactate dehydrogenase expressed in human cancer. | an unusual isozyme of lactate dehydrogenase (ldh; l-lactate:nad+ oxidoreductase, ec 1.1.1.27), ldhk, has been described in cells transformed by the kirsten murine sarcoma virus (kimsv). this isozyme appears to contain one or more subunits encoded by the transforming gene of kimsv and is readily distinguished from other isozymes of ldh. specifically, it is more basic than other ldh isozymes, has an apparent subunit structure of (35,000)4(22,000)1, is essentially inactive if assayed under a normal ... | 1981 | 6942426 |
| sequence of events in the transformation process in cells infected with a temperature-sensitive transformation mutant of moloney murine sarcoma virus. | normal rat kidney cells infected with the temperature-sensitive transformation mutant of moloney murine sarcoma virus were used to study the biochemical and morphological changes that occur during transformation. the infected cells exhibited a normal morphology at the nonpermissive temperature (39 degrees c) and a transformed morphology at the permissive temperature (33 degrees c). a new viral protein was detected 2 hr after shift to the permissive temperature as a polyprotein with an estimated ... | 1981 | 6946495 |
| virally induced periosteal osteogenesis in mice. | inoculation of moloney murine sarcoma virus (m-msv) into shank muscles of adult nmri mice resulted in localized sarcoma and periosteal membrane proliferation with subsequent periosteal bone formation. newly formed bone arose from outer surface of shank bones. two weeks after m-msv inoculation the width of bone cortex increased nearly 4 times. the spaces between newly formed bone ossicles were filled with bone marrow. in the later stages these bone marrow cavities were merged with medullar caviti ... | 1981 | 6783270 |
| complementary dna copies of leukemia and sarcoma virus rna contain sequences of deoxycytidylate and deoxyguanylate. | single-stranded complementary dna (cdna) of the rna of gazdar murine sarcoma virus, gz-msv/mulv; moloney murine leukemia virus, m-mulv; mouse mammary tumor virus, mmtv; and simian sarcoma virus, ssv-1, were synthesized in endogenous reverse transcriptase reaction. gz-msv/mulv cdna was also synthesized in exogenous in vitro reverse transcriptase reactions. in the endogenous reaction, 30-35s, or 6.0- to 7.9-kilobase-length cdna transcripts were synthesized in high yield. in comparison, transcripts ... | 1981 | 6299564 |
| t cell-mediated immunity to oncornavirus-induced tumors. iii. specific and nonspecific suppression in tumor-bearing mice. | a strong cell-mediated immune response against friend, moloney, rauscher virus-induced (fmr) cell surface antigens has been demonstrated previously in mice which reject oncornavirus-induced tumors. in order to identify an eventual suppressor mechanism in animals with progressively growing tumors, experiments were initiated in c57bl/6 mice bearing either a murine sarcoma virus (msv) tumor or moloney virus-induced lymphoma (mbl2). progressive tumor growth was induced (a) in viremic animals first i ... | 1981 | 6452278 |
| t cell-mediated immunity to oncornavirus-induced tumors. iv. preliminary evidence for a specific suppression of anti-moloney cell-mediated immune response by autoimmune t cells. | previous reports have demonstrated that adult c57bl/6 mice infected with murine sarcoma virus (msv) develop a strong cell-mediated immune response against friend, moloney, rauscher virus-induced type-specific (fmr) antigens and reject their tumors. to demonstrate a possible role for auto-anti-msv t blasts, syngeneic c57bl/6 mice were immunized with highly enriched anti-fmr cytolytic t cells. one of 3 pools of these autoimmune t cells prepared from 12 surviving immunized mice (a) inhibited specif ... | 1981 | 6452279 |
| systemic and in situ natural killer and suppressor cell activities in mice bearing progressively growing murine sarcoma-virus-induced tumors. | the activity of systemic and in situ natural killer (nk) cells was measured in 3- to 5-week-old cba mice with progressively growing murine sarcoma virus (msv)-induced tumors. splenic nk activity was depressed in tumor-bearing mice. nk activity was quite low or not detectable in unfractionated cell suspensions from the tumors 10-12 days after inoculation of virus, but activity was observed upon depletion of phagocytic and/or adherent cells. to determine whether this in situ inhibition of nk activ ... | 1981 | 6457000 |
| onc sequences (v-fes) of snyder-theilen feline sarcoma virus are derived from noncontiguous regions of a cat cellular gene (c-fes). | type c sarcoma viruses are genetic recombinants containing portions of replication-competent helper viruses linked to sarcoma virus-specific sequences (generically designated onc genes) which are thought to be required for acute fibroblast transformation. the onc elements of different avian and mammalian sarcoma viral isolates are each homologous to subsets of cellular dna sequences which have no well-defined role in normal cells. because of the lack of significant homology between helper viral ... | 1981 | 6259536 |
| morphological and functional differentiation of kirsten murine sarcoma virus-transformed rat adrenocortical cell lines. | this study was undertaken to examine relationships of the phenotype of malignant cells to target cell properties and to events early in the transformation process. eighteen transformed lines were obtained by kirsten murine sarcoma virus infection of cells from adrenal glands of rats ages 4 to 30 weeks, at first or second passages in culture. they were grown either as fibroblastic adrenocortical stem cells or as more differentiated epithelial cells, depending on culture conditions. of 14 lines ex ... | 1981 | 6260350 |
| glucocorticoid regulation of the ha-musv p21 gene conferred by sequences from mouse mammary tumor virus. | molecular chimeras with the p21 transforming gene of harvey murine sarcoma virus linked to dna containing the long terminal repeat (ltr) or mouse mammary tumor virus (mmtv) have been constructed. transformants of nih 3t3 cells induced by transfection with mmtv ltr-p21 hybrid dna have been identified that express the normal p21 gene product. the levels of p21 rna and protein in these transformants are regulated by physiological concentrations of dexamethasone, a synthetic glucocorticoid hormone. ... | 1981 | 6277498 |
| generation of balb-musv and ha-musc by type c virus transduction of homologous transforming genes from different species. | the nature of the cell-derived (bas) sequences of balb-musv, a spontaneous mouse sarcoma virus isolate, was determined. molecularly cloned bas sequences demonstrated no detectable homology with the onc genes of other mouse transforming viruses, but exhibited a high degree of sequence homology with the ras gene of the rat-derived harvey murine sarcoma virus (ha-musv) genome. the ha-musv cell-derived sequence (ras) shared a colinear 750 bp region of homology with bas. moreover, balb-musv transform ... | 1981 | 6276008 |
| analysis of transforming gene products from moloney murine sarcoma virus. | we previously showed that in vitro translation of m-musv virion rna yielded a 62 kd gag gene product and an overlapping set of four proteins with approximate molecular weights of 37,000, 33,000, 24,000 and 18,000. in this paper we show, by use of hybrid arrest translation with cloned recombinant dnas containing m-musv v-mosmo sequences, that the 37, 33, 24 and 18 kd proteins are synthesized in their entirety from the v-mosmo gene. analysis of the primary sequence of these proteins shows that eac ... | 1981 | 6276018 |
| neoplastic transformation of mouse fibroblasts by murine sarcoma virus: a multi-step process. | infection of cultures of three different normal rodent cell lines with murine sarcoma virus (msv) resulted in very rapid loss of contact inhibition of growth and morphological transformation. in the case of two of these lines, anchorage dependence of growth was also rapidly lost but with the 3rd, c3h10t1/2 c1.8, an established line of mouse embryo fibroblasts, there was a delay of many cell generations before the cells became anchorage independent. this was despite 100% successful infection, as ... | 1981 | 6268740 |
| ldhk, a uniquely regulated cryptic lactate dehydrogenase associated with transformation by the kirsten sarcoma virus. | a novel isozyme of lactate dehydrogenase is detected in various cells transformed by the kirsten murine sarcoma virus (kimsv). this isozyme, designated ldhk, is strongly inhibited by physiological concentrations of oxygen, in an apparently cooperative fashion. ldhk is inhibited by guanosine triphosphate and related compounds, in a noncompetitive fashion. ldhk is found with both 35,000- and 22,000-dalton subunits, although these probably cleave from a 57,000-dalton precursor. in studies utilizing ... | 1981 | 6270118 |
| construction and isolation of a transmissible retrovirus containing the src gene of harvey murine sarcoma virus and the thymidine kinase gene of herpes simplex virus type 1. | we constructed lambda recombinants containing the harvey murine sarcoma virus genome and the thymidine kinase (tk) gene of herpes simplex virus type 1 linked to each other. the tk gene was located in a position downstream from both the long terminal repeat and the src gene of harvey murine sarcoma virus. the dnas of the lambda recombinants were used to transfect nih3t3 mouse fibroblasts in order to obtain harvey murine sarcoma virus dna-induced foci of transformed cells. the transformed foci wer ... | 1981 | 6270359 |
| specific transcriptional initiation in vitro on murine type c retrovirus promoters. | we have investigated the ability of molecularly cloned murine type c retroviral dna to direct accurate initiation of rna synthesis when added to cell-free extracts. two different cloned proviruses were used. the first was derived from an integrated molecule of akr murine leukemia virus and contains adjacent host information. the origin of the second was an unintegrated permuted copy of harvey murine sarcoma virus. we found that the leukemia virus cloned provirus, as predicted by structural consi ... | 1981 | 6270679 |
| transformation of rat thyroid epithelial cells by kirsten murine sarcoma virus. | fischer rat thyroid epithelial cella (frt) growing continuously in culture were infected with the kirsten murine sarcoma virus kimsv(kimulv) and found to produce this virus constitutively. although the morphology of the frt cells did not change appreciably, the cells became malignant after infection with kimsv(kimulv) as shown by the growth of infected cels in semi-solid media (uninfected frt cells did not grow) and by the tumorigenicity of infected when injected into syngeneic animals (uninfect ... | 1981 | 6273340 |
| sv40 recombinant molecules express the gene encoding p21 transforming protein of harvey murine sarcoma virus. | 1981 | 6273733 | |
| mechanisms of kirsten murine sarcoma virus transformation-induced changes in the collagen phenotype and synthetic rate of balb 3t3 cells. | specific viral transformation rather than cell selection can explain the previously observed increase in the proportion of type iii procollagen compared to type i procollagen in balb 3t3 cells transformed by kirsten murine sarcoma virus (ki-msv). two subclones of balb 3t3 a31 were productively infected with with a temperature-sensitive ki-msv in the presence of helper murine leukemia virus (mlv), resulting in virtually complete transformation of cultures and eliminating selection of transformed ... | 1981 | 6273840 |
| avian reticuloendotheliosis virus: characterization of genome structure by heteroduplex mapping. | the genome structure of defective, oncogenic avian reticuloendotheliosis virus (rev) was studied by heteroduplex mapping between the full-length complementary dna of the helper virus rev-t1 and the 30s rev rna. the rev genome (5.5 kilobases) had a deletion of 3.69 kilobases in the gag-pol region, confirming the genetic defectiveness of rev. in addition, rev lacked the sequences corresponding to the env gene but contained, instead, a contiguous stretch (1.6 to 1.9 kilobases) of the specific seque ... | 1981 | 6262534 |
| transformation of cultured rat ovarian surface epithelial cells by kirsten murine sarcoma virus. | a method is described for the culture of rat ovarian surface epithelial cells, i.e., the cellular component thought to be the source of most ovarian cancers. these cells in culture have a characteristic epithelial morphology which distinguishes them from other ovarian cell types. cultured surface epithelial cells are histochemically positive for 17 beta-hydroxysteroid dehydrogenase and negative for delta 5-3 beta-hydroxysteroid dehydrogenase, the same as in cryostat sections of whole rat ovary. ... | 1981 | 6263459 |
| translational products of moloney murine sarcoma virus rna: identification of proteins encoded by the murine sarcoma virus src gene. | in vitro translation of virion rna of moloney murine sarcoma virus (msv) strain 124 yielded major products having molecular weights of 63,000 (63k), 43k, 40k, 31k, and 24k daltons. a molecularly cloned subgenomic fragment of moloney msv comprised of the cellular insertion (src) region was utilized in hybridization arrest translation as a means of identifying products of the msv src gene. msv src dna specifically inhibited synthesis of the 43k, 40k, 31k, and 24k proteins, implying that each of th ... | 1981 | 6264137 |
| regulation of cell-mediated immunologic reactivity to moloney murine sarcoma virus-induced tumors. i. cell and serum activity detected by leukocyte adherence inhibition. | cell-mediated immunity and serum regulatory factors were studied in an in vitro system involving a spontaneously regressing, virus-induced tumor. inbred balb/c and cba mice were inoculated with moloney murine sarcoma virus and their peritoneal cells were tested for reactivity in leukocyte-adherence inhibition tests with extracts of syngeneic tumors. sera from inoculated mice were tested for their effect on this reactivity. at the optimal dilution, tumor extracts induced significant reactions wit ... | 1981 | 6264193 |
| three distinct genes in human dna related to the transforming genes of mammalian sarcoma retroviruses. | southern blot hybridization was used to identify human and other vertebrate dna sequences that were homologous to cloned dna fragments containing the oncogenic nucleic acid sequences of three different type c mammalian retroviruses (simian sarcoma virus, the snyder-theilen strain of feline sarcoma virus, and the harvey strain of murine sarcoma virus). each onc gene counterpart has a single genetic locus, which probably contains non-onc intervening sequences. the human dna sequences may represent ... | 1981 | 6264598 |
| enhancement of antibody activity to bovine leukemia virus by complement. | cocultivation of fetal lamb kidney cells infected with bovine leukemia virus (blv) and the cat cells containing murine sarcoma virus genome resulted in the rapid production of syncytia. this syncytia formation was inhibited by serum containing antibodies to glycoprotein antigen of blv. when rabbit complement was added to the antiserum for early syncytia inhibition (esi) test, a significant enhancement of esi activity of the antiserum was observed. this enhancement was associated with igg fractio ... | 1981 | 6264895 |
| transforming growth factor production by chemically transformed cells. | evidence is presented indicating that the chemically transformed akr-mca and c3h/mca-58 murine cell lines produce "transforming growth factor(s)" capable of inducing a transformed morphology and the ability to grow in soft agar in nontransformed, anchorage-dependent indicator cells. serum-free medium conditioned by exposure to the chemically transformed cells was chromatographed on a bio-gel p-60 column after dialysis and lyophilization. using the nontransformed mouse akr-2b cells as the indicat ... | 1981 | 6265069 |
| in vivo antigenic modification of tumor cells. iii. metastatic thymic lymphoma specifically infected by thymotropic retrovirus. | tissue distribution of radiation leukemia virus (radlv) was examined after its inoculation into normal c57bl/6j (b6) mice, b6 mice bearing a transplantable, non-virus-producing thymic lymphoma (rl12-np), and b6 mice bearing a transplanted non-virus producing, harvey murine sarcoma virus-transformed fibrosarcoma. virus expression was determined by competition radioimmunoassay for murine leukemia virus (mulv) p30 (predominant group-reactive antigen of mulv) and for radlv p12 (a highly type-specif ... | 1981 | 6265678 |
| the p21 src genes of harvey and kirsten sarcoma viruses originate from divergent members of a family of normal vertebrate genes. | the harvey and kirsten strains of murine sarcoma virus encode enzymatically and serologically related p21 src proteins which are required for virally mediated cellular transformation. the genes in each virus encoding p21 show such extensive divergence from each other that cloned probes from these genes detect distinct sets of cellular genes in the dna from several vertebrate species. these data suggest that cellular p21 sarc genes constitute a divergent family of vertebrate genes that can regula ... | 1981 | 6265801 |
| host range of murine sarcoma virus, its host modification and phenotypic mixing with endogenous virus. | the m(msv)ag-50 cells produce competent ecotropic sarcoma virus which is able to transform several rodent embryo cells and replicate in them. the expanded host range has shown that the virus acquired some host information which facilitate the transformation of heterologous cells. the transformed cells were xc positive and with transformed phenotype in vitro. the genome of mouse sarcoma virus from nonproducer rat liver cells could be rescued by xenotropic endogenous virus. the obtained virus has ... | 1981 | 6265817 |
| transforming growth factors (tgfs): properties and possible mechanisms of action. | transforming growth factors (tgfs) are growth-promoting polypeptides that cause phenotypic transformation and anchorage-independent growth of normal cells. they have been isolated from several human and animal carcinoma and sarcoma cells. one tgf is sarcoma growth factor (sgf) which is released by murine sarcoma virus-transformed cells. the tgfs interact with epidermal growth factor (egf) cell membrane receptors. tgfs are not detectable in culture fluids from cells which contain high numbers of ... | 1981 | 6267317 |
| analysis of two divergent rat genomic clones homologous to the transforming gene of harvey murine sarcoma virus. | harvey murine sarcoma virus (ha-musv) is a mouse-rat recombinant retrovirus that encodes a protein designated p21, required for virally induced transformation. using a radiolabeled dna fragment from the p21 coding region, we have detected homologous dna sequences in the normal dna of rats and of several other vertebrate species. moreover, many tested cells from these species contain low levels of a p21 protein that is highly related to viral 21. now we report two independent fragments from norma ... | 1981 | 6267583 |
| t lymphocyte tolerance and early appearance of virus-induced cell surface antigens in moloney-murine leukemia virus neonatally injected mice. | regression of moloney-murine sarcoma virus- (m-msv) induced sarcomas in normal adult mice is accompanied by generation of virus-specific cytotoxic t lymphocytes (ctl). however, when neonatal mice that were injected with moloney-murine leukemia virus (m-mulv carrier) were subsequently challenged as adults with m-msv, the sarcomas did not regress nor did they generate ctl. this failure to produce ctl cannot be ascribed to nonspecific immunodepressive effects or to suppressor cell generation since ... | 1981 | 6969741 |
| quantitation and clonal isolation of cytolytic t lymphocyte precursors selectively infiltrating murine sarcoma virus-induced tumors. | a limiting dilution mixed leukocyte-tumor cell microculture system was used to quantitate cytolytic t lymphocytes and their precursors (ctl-p), which infiltrate tumors induced by injection of moloney sarcoma-leukemia virus (msv-molv) complex into c57bl/6 mice. leukocyte populations obtained from tumors collected on day 10 after virus injection were found to contain significantly higher frequencies of operationally defined (tumor-specific) ctl-p than either peripheral blood leukocytes (pbl) or sp ... | 1981 | 6973602 |
| a selective temperature-sensitive defect in viral rna expression in cells infected with a ts transformation mutant of murine sarcoma virus. | 1981 | 7273137 | |
| distribution of in vitro-stimulated immune lymphocytes in mice bearing moloney murine sarcoma virus-induced primary tumor. | the distribution of in vitro-stimulated immune lymphocytes in tumor-bearing mice was studied. spleen cells from balb/c mice which had regressed moloney murine sarcoma virus (m-msv)-induced primary tumors, were sensitized in vitro by using moloney murine leukemia virus-induced balb/c lymphoma (lstra). lymphocytes obtained 6 days after stimulation were examined for cytotoxic activity against lstra cells, labeled with 51 cr or 99mtc, and inoculated into mice bearing msv-induced primary tumors. the ... | 1981 | 7274651 |
| no activation of new initiation points for deoxyribonucleic acid replication in balb/c 3t3 cells transformed by kirsten sarcoma virus. | balb/c 3t3 cells were transformed by kirsten sarcoma virus, and five clones were isolated in soft agar. average replicon sizes of the transformed cell lines were estimated by the method of fiber-autoradiography (j. a. huberman and a. d. riggs, j. mol. biol.32:327-341, 1968) and found to be the same size as the nontransformed 3t3 cells, analyzed in parallel. the results indicate that, unlike simian virus 40 and epstein-barr virus, kirsten sarcoma virus does not activate new initiation points for ... | 1981 | 9279389 |
| biological, immunological, and molecular properties of revertants of cat cells transformed by murine sarcoma virus. | 1981 | 6257388 | |
| resistance to moloney murine sarcoma virus (m-musv) tumor induction is associated with natural antibody production to "endogenous" moloney leukemia virus (m-mulv) in balb/mo mice. | balb/mo mice are characterized by early expression of "endogenized" m-mulv and are resistant to m-musv tumor induction. furthermore, compared to normal balb/c mice, sera from balb/mo mice exhibit a significant reactivity which is specific for m-mulv when tested in a 125i-labelled staphylococcus protein a binding assay. the possible significance of this reactivity in conferring tumor resistance is explored. | 1981 | 6278685 |
| detection of a recombinant murine leukemia virus-related glycoprotein on virus-negative thymoma cells. | x-irradiation of outbred swiss mice resulted in the development of virus-free thymomas. when put in culture, a lymphoblastic cell line (nixt) expressed neither particles nor infectious virus but supported the growth of pure ecotropic murine leukemia viruses (mulvs) without generating any envelope recombinant (rm) mulv in more than 20 months of culture. these cells did not support the growth of rm-mulvs and completely excluded the entry of all rm-mulv pseudotypes of murine sarcoma virus, suggesti ... | 1981 | 6165021 |
| regulation of cell-mediated immunologic reactivity to moloney murine sarcoma virus-induced tumors. ii. nature of blocking and unblocking factors in serum. | serum blocking factors, detected in sera of inbred cba and balb/c mice with progressing virus-induced tumors by their interference with leukocyte-adherence inhibition (lai) with the use of syngeneic leukocytes, were found to be strain related: no blocking occurred with allogeneic leukocytes. furthermore, the unblocking factors of regressor serum were also strain related: no unblocking of allogeneic progressor serum was observed. there was no requirement for h-2 compatibility between leukocytes a ... | 1981 | 6165853 |
| interferon effect on collagen and fibronectin distribution in the extracellular matrix of murine sarcoma virus-transformed cells. | the transformation of murine balb/c embryonic fibroblasts by murine sarcoma virus, moloney strain, followed by prolonged treatment with murine interferon, resulted in the appearance of a new cell population (msv-if+). these msv-if+ cells are characterized by the recovery of a normal phenotype, contact inhibition, and lack of colony formation in agar. this phenotypic change of the msv-if+ cells is associated to the neosynthesis of a dense fibrous matrix beyond the cell periphery. ultrastructural ... | 1981 | 6167350 |
| purified low-molecular-weight protein kinase from murine sarcoma virus particles catalyzes tyrosine phosphorylation endogenously but phosphorylates cellular proteins at serine. | the low-molecular-weight (lmw) protein kinase associated with high-titer murine sarcoma virions have been extensively purified by ammonium sulfate fractionation. bio-gel p-100 gel filtration, deae-cellulose and carboxymethyl cellulose chromatography. the purified enzyme migrates as a 16k polypeptide in polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate. the enzyme catalyzes phosphotransfer with atp as a phosphate donor to various exogenously added proteins as acceptors; ... | 1981 | 6168778 |
| further studies on anti-tumor activity of adenovirus fibre protein on murine sarcoma tumours. | the present study demonstrates that subcutaneous injection of purified adenovirus 12 fibre protein (fp) to 4-week-old balb/c mice, 4, 2 and 1 day(s) before intramuscular injection of murine sarcoma virus (msv-m) or 2 and 1 day before and 1 day after msv-m injection causes significant inhibition of tumour growth (p less than 0.001). the evidence suggests that treatment with fp leading to tumour inhibition is due to neither an interferon mechanism nor to inhibition of virus multiplication of fp in ... | 1981 | 6169787 |
| in vitro proteolytic cleavage of gazdar murine sarcoma virus p65gag. | moloney murine leukemia virus, disrupted in concentrations of 0.1 to 0.5% nonidet p-40, catalyzed the cleavage of p65, the gag gene polyprotein of the gazdar strain of murine sarcoma virus, into polypeptides with sizes and antigenic determinants of murine leukemia virus-specified p30, p15, pp12, and p10. cleavage performed in the presence of 0.15% nonidet p-40 in water yielded polypeptides of approximately 40,000 (p40) and 25,000 (p25) mr. in vitro cleavage performed in a buffered solution conta ... | 1981 | 6169847 |
| complete nucleotide sequence and organization of the moloney murine sarcoma virus genome. | the complete nucleotide sequence of a mammalian transforming retrovirus. moloney murine sarcoma virus, has been determined. msv, recombinant virus derived of helper viral and cellular sequences, possesses termini resembling prokaryotic transposable elements. the viral genome has the coding capacity for the moloney murine leukemia virus gag gene product and contains large deletions in pol and env genes. a large open reading frame encompassing its cell-derived sequences codes for its putative tran ... | 1981 | 6170110 |
| partial reversion of the transformed phenotype of murine sarcoma virus-transformed cells in the presence of interferon: a possible mechanism for the anti-tumour effect of interferon. | the effect of interferon (ifn) on a series of cellular properties which characterize the transformed state was studied, using normal mouse fibroblasts (both established lines and embryo cells) and cloned derivatives transformed by murine sarcoma virus. it was found that the transformed cells behaved in a more normal fashion in the presence of ifn. this was indicated by reduction in saturation density, decreased dna synthesis in crowded cultures, decreased formation of foci on monolayers of norma ... | 1981 | 6171576 |
| nucleotide sequence of the genome of a murine sarcoma virus. | 1981 | 6173134 | |
| rapid syncytium formation induced by moloney murine sarcoma virus in 3t3/nih cells and its delay by mouse interferon. | moloney murine sarcoma virus (msv) clone 124 was found to induce rapid syncytium formation upon infection of 3t3/nih at a high multiplicity of infection. this effect became apparent, by light microscopy, within 1 to 2 hours, whereas by scanning electron microscopy, clusters of 4 to 25 cells were seen in their initial steps of syncytium formation within 20 to 30 minutes after virus addition. it appeared that the cell fusion was initiated by connection between the cells through virus bound to thei ... | 1981 | 6174107 |
| use of hybridoma-resident variant cell lines to study h-2db/fmr-specific cytotoxic t cells. | an h-2db heterozygous tumor cell line and a variant subclone bearing a mutant gene product were used to analyze the h-2db specificity of cytotoxic t lymphocytes (ctl) generated during a moloney murine sarcoma virus (msv) infection. when the mutant cells were used as targets for msv-specific ctl, the amount of cell lysis, compared with that seen with the nonmutant parental cells, was drastically decreased. however, cells of the mutant clones remained susceptible to allogeneic ctl specific for the ... | 1981 | 6174439 |
| reorganization of the cytoskeleton by interferon in msv-transformed cells. | beta and gamma interferons were found to enhance the organization of microfilaments, intermediate filaments, and fibronectin in the murine sarcoma virus (msv)-transformed cell line. furthermore, both interferons increased the number of intermediate-type junctions. sodium butyrate was also found to act in a similar way and, when associated with interferon, an additive effect was observed. in the cell line, cultured for many passages in the presence of beta interferon (msv-if+), the cytoskeletal n ... | 1981 | 6180063 |
| harvey and kirsten sarcoma viruses promote the growth and differentiation of erythroid precursor cells in vitro. | harvey and kirsten murine sarcoma viruses have previously been shown to transform fibroblastic cells in culture, and type c virus pseudotypes of these viruses cause erythroleukemia in susceptible mice. we report a cell culture assay for quantitating the growth-promoting effect of harvey and kirsten viruses on erythroid cells. murine hemopoietic cells were infected in vitro with harvey or kirsten sarcoma virus, and then cultured in methylcellulose in the presence of relatively low concentrations ... | 1981 | 6276011 |
| migration of null lymphocytes to murine sarcoma virus-induced tumors. | 1981 | 6266725 | |
| induction of surface qa2 on lymphoid cells. | three tumor cell lines isolated from individual murine sarcoma virus-infected balb/cby (qa2-) mice were established. boms, ora i, and thorbly i were analyzed for the expression of qa2 and ly surface differentiation antigens. by cytofluorograf analysis and absorption techniques, all 3 cell lines were found to be qa2+, ly1-, ly2-. this cell surface phenotype suggests that immature lymphoid cells are uniquely susceptible to transformation by virus. anomalous appearance of qa2 on transformed lymphoi ... | 1981 | 7229374 |
| identification of unintegrated forms of kirsten murine sarcoma viral dna and restriction endonuclease cleavage map of linear dna. | we detected unintegrated linear 7.0-kilobase pair dna and covalently closed circular dna species in nih3t3 cells recently infected with kirsten murine sarcoma virus. using the linear dna, we constructed a restriction endonuclease cleavage map and compared it with the map of harvey murine sarcoma virus. the restriction endonuclease maps of two segments, one 1.2 kilobase pairs (smai site) to 3.7 kilobase pairs (hindiii site) from the right end (corresponding to the viral 3' side) and the other 0.5 ... | 1981 | 6264147 |
| structure and functions of the kirsten murine sarcoma virus genome: molecular cloning of biologically active kirsten murine sarcoma virus dna. | the unintegrated closed circular form of viral dna prepared from nih3t3 cells infected with kirsten murine sarcoma virus was cloned into bacterial plasmid pbr322. the closed circular dna, which consisted of two different-sized populations, was enriched from the virus-infected cells, linearized with bamhi, and inserted into pbr322 dna. four different recombinant dnas (clones 2, 4, 6, and 7) were obtained, and a physical map of each was constructed by using various restriction enzymes. clone 4 dna ... | 1981 | 6264139 |
| comparative effects of azathioprine, cyclophosphamide and frentizole on cellular immunity in mice. | this report extends previous observations on the immunosuppressive properties of cyclophosphamide (cpa), azathioprine and frentizole (15) to include murine models of cellular immunity. systemic and local graft vs host reactions (gvhr) were most effectively suppressed by cpa. in contrast to frentizole, both cpa and azathioprine were found to inhibit the proliferation of parental t-cells in a systemic gvhr. however, cpa was the only agent capable of inhibiting the proliferation of t-cells followin ... | 1981 | 6978364 |
| growth properties and susceptibility to viral transformation of skin fibroblasts from individuals at high genetic risk for colorectal cancer. | growth characteristics and susceptibility to viral transformation were compared in cultured skin fibroblasts from patients with gardner's syndrome (gs), those with familial polyposis coli (fp), asymptomatic family members, and unrelated controls. compared to cells from unrelated controls, cells from 4 of 5 gs patients were transformed by kirsten murine sarcoma virus at 100- to 1,000-fold increased efficiency. the transformation efficiencies of fibroblasts from 2 of 3 fp patients were 10- to 100- ... | 1981 | 6935464 |
| markedly elevated levels of an endogenous sarc protein in a hemopoietic precursor cell line. | the src gene product of harvey murine sarcoma virus is a 21,000-dalton guanine nucleotide-binding protein. we have recently shown that a wide variety of vertebrate cell strains and cell lines express much lower levels of an endogenous p21 immunologically related to the harvey murine sarcoma virus-coded p21. in this report, we have examined the levels of endogenous p21 in a unique hemopoietic precursor cell line, 416b, which was originally described as a continuous cell line of a hemopoietic stem ... | 1981 | 6821513 |
| synergistic interaction of two classes of transforming growth factors from murine sarcoma cells. | transforming growth factors (tgfs) isolated from murine sarcoma virus-transformed 3t3 cells have been separated by high-pressure liquid chromatography into two subsets. one subset, called tgf alpha, competes with epidermal growth factor (egf) for receptor sites, whereas the other, called tgf beta, does not. tgb beta, purified by high-pressure liquid chromatography, will not induce formation of large colonies of cells in soft agar in the absence of tgf alpha or egf. however, the combined action o ... | 1982 | 6290046 |
| characterization of the phosphorylation sites and the surrounding amino acid sequences of the p21 transforming proteins coded for by the harvey and kirsten strains of murine sarcoma viruses. | the transforming protein coded for by the onc gene (v-rasha) of harvey murine sarcoma virus (ha-musv) is the 21,000-dalton protein (p21) which is the immediate agent responsible for the virus-induced malignant transformation of normal cells. the p21 proteins of ha-musv and the closely related kirsten murine sarcoma virus are heavily phosphorylated in vivo. in the partially purified ha-musv p21, the protein shows a guanine nucleotide-binding activity and, in addition, a very unique autophosphoryl ... | 1982 | 6288698 |
| nucleotide sequence of the p21 transforming protein of harvey murine sarcoma virus. | harvey murine sarcoma virus is a retrovirus which transforms cells by means of a single virally encoded protein called p21 has. we have determined the nucleotide sequence of 1.0 kilobase in the 5' half of the viral genome which encompasses the has coding sequences and its associated regulatory signals. the nucleotide sequence has identified the amino acid sequence of two additional overlapping polypeptides which share their reading frames and the carboxyl termini with p21 but which contain addit ... | 1982 | 6287572 |
| phase ii evaluation of 4'-epi-doxorubicin in patients with advanced colorectal carcinoma. | 4'-epi-doxorubicin (4'-epi-dx) is a new doxorubicin derivative that is more active than the parent compound against murine sarcoma virus tumors and lewis lung carcinoma and may be less toxic. thirty-five patients with advanced measureable colorectal carcinoma were treated with 4'-epi-dx (85 mg/m2) every 3 weeks. the major toxic effect was leukopenia less than 2000 cells/mm3 in 28% of the patients. one of 29 patients (3%) had a partial response. at this dose and schedule, 4'-epi-dx has minimal ac ... | 1982 | 6956441 |
| glycolipid n-acetylgalactosaminyltransferase activities in normal and kirsten murine sarcoma virus transformed balb/c 3t3 cells. | 1982 | 6814199 | |
| analysis of v-mos encoded proteins in cells transformed by several related murine sarcoma viruses. | we have used antisera against synthetic peptides to identify and characterize a 37,000 dalton v-mos encoded protein (p37mos) in cells transformed by m-musv 124. p37mos, a phosphoprotein, comprises only about 0.0005% of total cellular protein in cell lines transformed by m-musv 124. nih 3t3 cells acutely infected with m-musv 124, however, contain 30-100-fold more p37mos. these elevated levels of p37mos correlate with striking morphological changes and cell death in the acutely infected cell popul ... | 1982 | 6298255 |
| identification of proteins encoded by the gazdar murine sarcoma virus genome by in vitro translation and comparison with moloney murine sarcoma virus 124. | the gene products of gazdar murine sarcoma virus (gz-musv) were identified by in vitro translation of gz-musv virion rna. an overlapping set of proteins with approximate molecular weights of 37,000 (37k), 33k, 24k, and 18k were synthesized from the transforming gene of gz-musv, v-mosgz. in addition, gz-musv-specific rna directed the in vitro synthesis of a 62k gag gene protein and a 37.5k env gene-related product. the gz-musv-specific in vitro translation products were compared with the in vitro ... | 1982 | 6180181 |
| viral-versus non-viral-induced yolk sac tumors in the rat. | yolk sac tumors were induced in fetectomized rats by displacement of the visceral yolk sac outside the uterus with or without injection of murine sarcoma virus (msv) into the placenta. some of the animals were immunized with msv prior to surgery. the biological, morphological and immunological characteristics of the tumors which develop in animals either injected or not with virus are compared. the results indicate that msv inoculation into the placenta increases the tumor incidence, shortens th ... | 1982 | 6181488 |
| induction of gp70-specific suppressor t-cells in mice inoculated with virus-producing tumor cells. | inoculation of balb/c mice with syngeneic, murine leukemia virus (mulv)-producing (murine sarcoma virus-transformed) balb/3t3 tumor cells resulted in diminution of alloreactivity as measured in the mixed leukocyte culture (mlc) reaction. cells that suppressed this response were identified in the spleens of tumor-bearing mice 10--14 days after inoculation. the suppressor cells were adherent, radiosensitive t-lymphocytes of the lyt 1-, 2, 3+ phenotype. mice inoculated with gross mulv (g-mulv)-prod ... | 1982 | 6214655 |
| dihydrocytochalasin b disorganizes actin cytoarchitecture and inhibits initiation of dna synthesis in 3t3 cells. | dihydrocytochalasin b (h2cb) disrupts the actin structure of swiss/3t3 mouse fibroblasts and inhibits the ability of serum growth factors to stimulate dna synthesis in quiescent cultures. low doses of h2cb (2-10 x 10(-7) m) added to serum-arrested cells reversibly block initiation of dna synthesis by serum; by epidermal growth factor and insulin; or by epidermal growth factor, fibroblast growth factor and insulin. h2cb is effective only when added to cells within 8-10 hr after stimulation. low d ... | 1982 | 6215122 |
| genetic control of sensitivity to moloney leukemia virus in mice. vi. involvement of virus-specific t helper cells collaborating with b cells. | t cell responses to moloney virus involve cytolytic and helper lymphocytes. in contrast to specific cytolytic t lymphocytes, few studies have been devoted to the characteristics of helper t cells for antibody production. the present experiments describe an assay for moloney virus-specific help for b cells using dinitrophenylated virus. this method, using the moloney virus as a carrier in a hapten-carrier system, allows to definition of the specific helper function of antibody responses. t helper ... | 1982 | 6216112 |
| clonal analysis of the t lymphocyte response to murine sarcoma virus-induced tumors. | 1982 | 6217605 | |
| diverse effects: augmentation, inhibition, and non-efficacy of 12-o-tetradecanoylphorbol-13-acetate (tpa) on retrovirus genome expression in vivo and in vitro. | the action of 12-o-tetradecanoylphorbol-13-acetate (tpa) on several retrovirus-related functions was investigated in four virus-host cell systems. the following effects were recorded: (i) in stu-mice, infected with the friend virus complex (friend) murine leukaemia virus/friend spleen focus forming virus) and treated with tpa (50 ng/g) for one week prior to infection, the number of spleen foci increased 5-fold over the control. (ii) addition of tpa (0.04 to 40 ng/ml) to virus-producing cell syst ... | 1982 | 6177439 |
| carcinogen- and radiation-transformed c3h 10t1/2 cells contain rnas homologous to the long terminal repeat sequence of a murine leukemia virus. | carcinogen- or radiation-transformed c3h 10t1/2 murine fibroblasts transcribe a set of poly(a)+rnas that contain sequences homologous to the long terminal repeat (ltr) sequence of moloney murine sarcoma virus. these ltr-containing rnas consist of a series of discrete bands ranging in size from about 38 to 18 s. the higher molecular weight molecules (30-38 s) in this set of rnas also contain sequences homologous to the gag, pol, and env genes of a murine leukemia virus. a 24s rna contains sequenc ... | 1982 | 6178111 |
| p65 of gazdar murine sarcoma viruses contains antigenic determinants from all four of the murine leukemia virus (mulv) gag polypeptides (p15, p12, p30, and p10) and can be cleaved in vitro by the mulv proteolytic activity. | 1982 | 6178211 | |
| moloney murine sarcoma virus tumors in cba/j mice: chemopreventive and chemotherapeutic actions of supplemental beta-carotene. | decreased tumor frequency, increased latent period, and increased rate of tumor regression were observed in male inbred cba/j mice fed supplemental beta-carotene before and/or after they were inoculated with the moloney sarcoma virus. when beta-carotene feeding was begun after tumors were already present, it markedly increased the rate of tumor regression. beta-carotene minimized the virus-induced thymus gland involution that accompanies tumor growth, and this action on the thymus gland was beli ... | 1982 | 6279952 |
| human nucleotide sequences related to the transforming gene of a murine sarcoma virus: studies with cloned viral and cellular dnas. | a recombinant plasmid, pi26, has been constructed by cloning into pbr322 a transforming gene of murine sarcoma virus (a moloney strain, clone 124, msv) synthesized by detergent-treated virions. from this plasmid a xbai-hindiii fragment has been isolated which contains only mos-specific sequences. this mos-specific probe has been used for screening a human gene library cloned in bacteriophage lambda charon 4a. of these, 19 clones have been isolated containing mos-related sequences. by physical ma ... | 1982 | 6281133 |
| long terminal repeat of murine retroviral dnas: sequence analysis, host-proviral junctions, and preintegration site. | the nucleotide sequence of the long terminal repeat (ltr) of three murine retroviral dnas has been determined. the data indicate that the u5 region (sequences originating from the 5' end of the genome) of various ltrs is more conserved than the u3 region (sequences from the 3' end of the genome). the location and sequence of the control elements such as the 5' cap, "tata-like" sequences, "ccaat-box," and presumptive polyadenylic acid addition signal aataaa in the various ltrs are nearly identica ... | 1982 | 6281466 |