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[rna tumor virus-like particles in hodgkin's disease and in non-hodgkin lymphomas]. 197777100
mouse mammary tumor virus production stimulated by hormones and polyamines in cells grown in semi-synthetic in vitro conditions.hormonal regulation of mouse mammary tumor virus (mmtv) synthesis was studied in the ccl-51-sf cell subline derived from the sykes' mammary tumor cell line ccl-51 and adapted to grow in semi-synthetic in vitro conditions. the virus was quantitated by measuring the supernatant reverse transcriptase activity in exogenous reaction using poly (ra)-oligo (dt) and poly (rc)-oligo (dg) as template/primers. the cells produced a low but significant amount of virus in the absence of any hormones and serum ...197765340
precursor-product relationship between nonglycosylated polypeptides of a and b particles of mouse mammary tumor virus. 197765829
replication of mouse mammary tumor virus in tissue culture. 1. establishment of a mouse mammary tumor cell line, virus characterization, and quantitation of virus production. 197765833
the structure of the mouse mammary tumor virus: isolation and characterization of the core. 197765834
characterization of a terminal deoxynucleotidyl transferase activity in mouse mammary tumor virus. 197765836
estrogens, estrogen receptors, and biological responses in experimental animals. 197778861
infection of cultured rat hepatoma cells by mouse mammary tumor virus.a continuous line of buffalo rat hepatoma (htc) cells has been successfully infected with mouse mammary tumor virus (mmtv) produced by the gr mammary tumor cell line. uniform infection required initial exposure of the htc cells to greater than 10(5) mmtv particles per cell. the resultant chronically infected cell population was found to have stably acquired 20-30 copies of mmtv dna. the infected cells contain viral rna and express viral antigens; however, very few mmtv particles are released int ...1977189932
mouse mammary tumor virus dna in infected rat cells: characterization of unintegrated forms.mouse mammary tumor virus (mmtv) dna in chronically infected rat hepatoma cells is maintained in both the integrated and unintegrated state. fractionation of dna by the procedure of hirt (1967) as well as by sedimentation through alkaline sucrose suggests that about two thirds of the viral dna is associated with high molecular weight cell dna. the remainder of the viral dna is unintegrated and is present primarily as linear or open circular duplexes consisting of a genome-length strand complemen ...1977189933
inhibition of mammary tumors by incomplete t-cell depletion.the effects of neonatal and perinatal thymectomy on mammary tumorigenesis in (c57bl x i)f1fc3h hybrid female mice were determined. when hybrid females were neonatally thymectomized by controlled suction, a procedure that removes thymic lobes completely, a large proportion of animals developed stigmas of a fulminant wasting disease and died before tumors developed. however, when hybrid females were subjected to neonatal thymectomy by continuous suction, a procedure that resulted in retention of t ...1977190416
quantitative determination of mammary tumor virus in individual samples of mouse milk.the possibility to determine quantitatively the intact mammary tumor virus (mtv) in milk of mice carrying milk-transmitted mtv has been assayed by a method that allows direct comparison between individual milk samples. the method is based on (a) the measure of light scattering of partially purified mtv preparations, (b) the use of milk from genetically identical mtv free mice as blank and (c) the quantitative reference to the total protein content of whole milk. the sensitivity, specificity and ...1977203070
quantitative comparison of milk-released c3h and riii mammary tumor viruses in infected balb/c hosts.the c3h and riii mammary tumor viruses (mtv) carried by balb/cfc3h and balb/cfriii breeding females have been quantified and compared in milk samples, after partial purification with a sucrose density gradient. the samples were collected at identical times during the first 3 lactation periods from individual mice (6 per strain), standardized for age at delivery and size of litter. milk samples from 6 mtv negative balb/c controls have also been analyzed. data for comparison are expressed in optic ...1977203071
comparative effects of diphenylglioxal and its superoxide on experimental tumors. 1977207230
immunologic studies of the low molecular weight dna binding protein of murine oncornaviruses.a low molecular weight, highly basic dna-binding protein was purified from several oncornaviruses by the sequential procedures of gel filtration in guanidine-hydrochloride, deae-cellulose chromatography and affinity chromatography on single-stranded dna sepharose. the binding protein from rauscher and woolly monkey type-c viruses was the fastest migrating of the virion proteins in sds-polyacrylamide gels and thus is designated p10 according to previous convention although our estimates of molecu ...1977194848
effect of immune manipulation on natural immune responses to murine mammary tumor antigens.the virus-host relationship in balb/cfc3h virgin female mice neonatally infected with an oncogenic virus (mammary tumor virus) (mumtv) was significantly altered by a brief immunosuppressive treatment during young adult life. natural immune responses were augmented and changed. the ability of lymphoid cells to attack target cells expressing mumtv-associated antigens was significantly increased, and a new component, a non-t-cell reactivity, was added to the t-cell reactivity found in normal female ...1977195069
electrophoretic analysis of the molecular weight of murine mammary tumor virus rna.molecular weight determinations of native and subunit rnas of murine mammary tumor virus (mumtv), a type b oncornavirus, were performed by polyacrylamide gel electrophoresis and compared with molecular weights of well-characterized avian cellular rnas and tobacco mosaic virus rna. from extrapolations of semilog plots of the molecular weights of the standard rnas versus relative electrophoretic mobilities and ferguson plots, the subunit and native rnas of mumtv were found to possess molecular wei ...1977195092
the major polypeptides of the murine-mammary-tumor virus isolated by plant-lectin affinity chromatography.solubilized polypeptides of the murine mammary tumor virus (mumt virus) were chromatographed on a column of immobilised concanavalin a. the unbound viral material was rechromatographed on phosphocellulose, resulting in the isolation of the major proteins with a molecular weight of 28000 (p28) and 12000 (p12) respectively. the adsorbed glycopolypeptides after elution with methyl alpha-d-mannopyranoside were subjected to gel filtration. the major glycoprotein with a molecular weight of 52000 (gp52 ...1977195815
biological activities of murine mammary tumour virus in vitro. increased macromolcular synetheses in mouse and hamster kidney cells; production of b- and c-particles in the mouse cells. 1977195818
simultaneous chemical induction of mtv and mlv in vitro. 1977195819
identification of the structural proteins of the murine mammary tumor virus that are serologically related to the antigens of intracytoplasmic type-a particles. 1977196403
murine mammary tumor virus protein vaccine: induction of antivirus immunity and inhibition or acceleration of growth of transplanted mammary tumors. 1977197247
glucocorticoid-stimulated accumulation of mouse mammary tumor virus rna: increased rate of synthesis of viral rna.glucocorticoid hormones specifically increase the intracellular concentration of mouse mammary tumor virus (mmtv) rna in a cultured cell line from a gr mouse mammary carcinoma (gr) and in an mmtv-infected rat hepatoma cell line (m1.19). in contrast, these steroids have no effect on the concentration of mmtv rna in a lymphoma line, s49, from a balb/c mouse. using a molecular hybridization procedure to detect newly synthesized rna, we have directly measured the effect of dexamethasone, a synthetic ...1977197523
pseudotypes of vesicular stomatitis virus with envelope antigens provided by murine mammary tumor virus. 1977197701
regulation of mammary tumor virus production by prolactin in balb/cfc3h mouse normal mammary epithelial cells in vitro.hormonal regulation of mammary tumor virus (mtv) production, has been analyzed with normal mammary epithelial cells from chronically infected balb/cfc3h mice. the effect of prolactin in terms of increased mtv production was not reproducibly observed in cells cultured in tissue culture dishes, whereas the cells grown on floating collagen gels consistently responded to prolactin in a dose-dependent manner. of the three media tested. dulbecco's modified eagle's medium was found to be the best in te ...1977198118
factors interfering with cellular immunological responses to the murine mammary tumor virus in tumor-bearing mice.t-cell-enriched spleen cell fractions from balb/c mice bearing virally induced mammary tumors become unresponsive with progressive tumor growth in the lymphocyte blastogenesis test, using purified mammary tumor virus (mtv) as an antigen. reactivity can be restored by mild trypsinization or extensive washing of the cells. when reactive cells from mice immunized with mtv were incubated with the wash fluid of the initially unresponsive cells. mtv-specific blastogenesis was inhibited. washings of no ...1977198383
comparison of mouse mammary tumor virus-specific dna in inbred, wild and asian mice, and in tumors and normal organs from inbred mice. 1977198552
elution of tumor-directed antibody from kidneys of mammary tumor-bearing mice.immunoglobulin-containing eluates, prepared from kidneys of paris riii mammary tumor-bearing mice approximately 10 months old, were incubated with frozen sections of mouse mammary tumor and normal lactating mammary gland and examined by immunofluorescence. the eluates diffusely strained the tumor but did not stain the nonneoplastic lactating mammary gland. mammary tumor and normal lactating mammary gland were both stained by an antiserum to mammary tumor virus (mumtv). the euglobulin portion of ...1977198566
transmission of mammary tumor virus in mouse strain dd: further support for the uniqueness of strain gr.outcrosses between high tumor mouse strain dd and low-incidence strains balb/c and c57bl to produce reciprocal f1 susceptible offspring of both types resulted in high incidences of mammary tumors in (dd female x balb/c male)f1 and (dd female x c57bl male)f1 females but not in the reciprocal hybrids with dd as the male parent. the finding that transmission of murine mammary tumor virus (mumtv) in dd was through the milk, as most often observed in mouse strains, further supported the uniqueness of ...1977198570
the major structural proteins of murine mammary tumor virus: techniques for isolation. 1977199080
phospholipids of the milk-fat-globule membrane and the mouse mammary-tumour virus isolated from the milk of infected mice [proceedings]. 1977199491
paternal transmission of mammary tumor virus from dd/tbr strain of mice by crossing with balb/c or c57bl/6j strains. 1977199517
development of a congeneic line of the gr mouse strain without early mammary tumours.based on the previous observation that mtv-2 controls the appearance of pregnancy-dependent mammary tumours and the early expression of mammary tumour virus (mtv)-antigens in the milk, an attempt was made to develop a congeneic gr line, without this locus, by introducing genetic material of the c57bl/10 strain. the cross-intercross system was used for this purpose. the mice of the even-numbered generations were selected for the absence of the mtv-2 locus with the early maammary tumour (emt) test ...1977199548
considerations on the preneoplastic lesions of the mammary gland.the general characteristics of the preneoplastic lesions of the human mammary gland, as they are known through histologic description, are outlined, and data obtained from the experimental analysis of mammary gland preneoplasia in five areas of endeavor are discussed. results obtained with transplantation procedures and aimed at defining the growth potential of hyperplastic outgrowths are reported. information derived from the study of events able to induce benign hyperplastic outgrowths or thei ...1977200144
radioimmunoassay for glycoprotein gp47 of murine mammary tumor virus in organs and serum of mice and search for related antigens in human sera.murine mammary tumor virus main glycoprotein (gp47), prepared by diethylaminoethyl cellulose and hydroxyapatite chromatography of detergent-mercaptoethanol-kci-disrupted virion, was used as labeled antigen in a highly specific and reproducible radioimmunoassay. seven other (glyco) proteins of the virus were antigenically distinct from gp47. serum and organs of unifected c57bl mice did not contain gp47, but sera of infected swiss and riii mice did contain the antigen. despite the high content in ...1977200346
long-term effects of prenatal and neonatal administration of 5beta-dihydrotestosterone on normal and neoplastic mammary development in mice.the long-term effects on mammary glands of 5beta-dihydrotestosterone, considered to be biologically inactive, were studied in female shn mice with mammary tumor, virus. 5beta-dihydrotestosterone was adminstered to mothers for 4 days from day 12 to day 15 of pregnancy (prenatal treatment) and to pups for 5 days of postnatal life (neonatal treatment) at daily doses of 1 mg and 200 mug, respectively. neonatal treatment resulted in marked stimulation of spontaneous mammary tumorigenesis; all neonata ...1977200350
relative importance of genotype and type of mammary tumor virus on mammary tumorigenesis in mice. 1977200431
evidence for an influence of mammary tumour virus on prolactin secretion in the mouse. 1977200691
mammary tumors and mammary tumor virus expression in hybrid mice of strains c57bl and gr.mammary tumorigenesis in genetic crosses between the high mammary tumor incidence gr and the low incidence c57bl mouse strains is highly correlated with murine mammary tumor virus expression in milk. although the f1 and first backcross females had a mammary tumor incidence which was consistent with a single dominant gene segregation, the tumor incidence in the critical second backcross segregants disproved the single gene hypothesis. genetic factors were clearly involved in regulation of virus e ...1977200697
[increase in the frequency of occurrence of mammary neoplasms in rats infected with mouse bittner virus]. 1977201438
[studies on reproduction and pregnancy--dependent mammary tumors of gr/a mice (author's transl)]. 1977201476
immunopathology of breast cancer. 1977201905
cellular immune responsiveness of breast cancer patients to parsi human milk particles & mammary tumor virus: lymphocyte blastogenesis. 1977202562
[effect of rna on tumor growth in mice of lines with and without the milk factor]. 1977188616
steroid induction of mouse mammary tumor virus: effect upon synthesis and degradation of viral rna.steroid hormones have been demonstrated to induce in tissue culture the production of mouse mammary tumor viral (mmtv) rna, proteins, and particles 10-fold compared with constitutive levels. however, previous data of increased viral rna levels did not distinguish between an increased rate of viral-specific rna synthesis and a slower rate of viral rna degradation. according to the recently developed assay of coffin et al. (1974) for measuring rates of viral rna synthesis, short-term labeling expe ...1977189061
application of freeze-drying intact cells to studies of murine oncornavirus morphogenesis.using a method for freeze-drying intact cells, uninfected and murine leukemia virus (mulv)-infected jlsv9 cell surfaces, as well as murine mammary tumor virus (mumtv)-infected cell surfaces, were examined by electron microscopy. the 10-nm knobs of mulv and the 5-nm spikes of mumtv were clearly revealed on the surfaces of budding viruses and were also found dispersed over the cell surface. the mulv knobs are randomly arranged on the virus surface, whereas the mumtv spikes are much more ordered. b ...1977189070
cyclic amp regulation of mammary tumor virus production.addition of dibutyryl cyclic amp (camp) and agents (isoproterenol and epinephrine) that stimulate the activities of adenylate cyclase enhance the stimulation of mammary tumor virus (mtv) production two- to threefold by glucocorticoid in short-term primary cultures of mammary tumors. this camp potentiation seems to depend on the stimulated level of mtv production by glucocorticoid alone, which increases mtv production 5- to 10-fold over basal level but varies greatly in absolute terms. when the ...1977189095
structural components of mouse mammary tumor virus. i. polypeptides of the virion. 1977190773
replication of mouse mammary tumor virus in tissue culture. ii. kinetics of virus production and the effect of rna and protein inhibitors on viral synthesis. 1977190785
the role of leukocyte subpopulations in the indirect leukocyte adherence inhibition assay in the mammary tumor virus system.a modification of the leukocyte adherence inhibition test for the detection of cellular immunologic reactivity of mice to the mammary tumor virus (mtv) has been described. it involves the transfer of the leukocyte adherence inhibition factor (laif) produced by spleen cells from immunized animals when cultured with antigen to indicator cells, for which peritoneal exudate cells from normal mice are used. the method proves to be sensitive and highly reproducible. by crude separation of leukocyte su ...1977191259
genetic transmission of mammary tumour virus by gr mice.by immunodiffusion assay (id-test) milk samples of mice of several strains and of f1-hybrids of the gr strain were tested for the presence of mammary tumour virus (mtv) antigens. the results clearly demonstrated that the presence of viral antigens in the milk of the first lactation period is restricted to mice harbouring endogenous mtv-gr. viral antigens were detectable in about 50% of the milk samples collected during the first (occasionally the second) lactation periods of mice of the segregat ...1977191409
naturally occurring lymphocyte reactivity to purified mammary tumor virus antigens. 1977191607
natural antibody in mammary tumor virus-infected mice that reacts with intracytoplasmic a particles of mouse mammary tumors.by an indirect immunofluorescence technique with prolonged serum incubation on murine mammary tumor (mt) slices, 179 of 424 mice examined were found to possess natural serum antibody (antibodies) that reacted with intracytoplasmic a particles (iap) of mt cells. the immunologic specificity of this antibody was supported by absorption and blocking experiments. furthermore, a strong similarity was seen between the mouse antibody reaction on various mt and the fluorescence pattern of rabbit anti-iap ...1977191630
isolation of the mouse mammary tumor virus sequences not transmitted as germinal provirus in the c3h and riii mouse strains.radioactive 60-70s rna from the mouse mammary tumor virus (mmtv) produced by the c3h mouse mammary tumor cell line (mm5mt) hybridized to a greater extent, and at a lower cot1/2 value, to the dna of c3h mammary tumor cells than to the dna of c3h liver cells. the 125i-labeled mmtv (c3h) 60-40s rna was annealed to a vast excess of dna from c3h livers, and single-stranded rna was eluted from hydroxylapatite and recovered. this "recycled rna" did not hybridize to the dna of the apparently normal orga ...1977191657
procedures for radioimmunoassay of the mouse mammary tumor virus.a procedure for radioimmunoassay of the major glycoprotein antigen derived from murine mammary tumor virus is described. the assay is sensitive to 0.05 ng of antigen and is highly reproducible. the antigen, gp55, has been found to be group specific and will detect viruses in 13 separate mouse strains, as wel .l as from continuous cell lines. factors affecting the assay have been examined.1977192451
plasma levels of a viral protein as a diagnostic signal for the presence of mammary tumor: the effect of tumor removal.we have previously shown (1, 2) that mice with mammary tumors can always be identified by their very high plasma levels of gp52, a 52,000 mol wt glycoprotein of the mouse mammary tumor virus (mmtv). the present investigation demostrates that the tumor is the principal source of the plasma gp52 since surgical excision is invariably followed in the first 9 days by a sharp decreasing (10-100-fold) of the gp52 levels. control animals in which the tumors were left in place by a "sham" surgical proced ...1977192830
the role of oncogenic viruses in neoplasia. 1977193432
levels of mammary tumor virus in hormone-dependent and -independent mouse mammary tumor cells.levels of mammary tumor virus particles (types a and b) and levels of the virus antigen were assayed in hormone-dependent and -independent mammary tumors of gr mice. various transplant generations of seven separate tumor lines were investigated. the results indicated that the tumors consisted of different cell clones, each of which exhibited a separate progressive expression and subsequent loss of the mammary tumor virus. when the tumors were transplanted, levels of b particles first declined in ...1977193632
immunoelectron microscopic studies of antibodies in mouse sera directed against mouse mammary tumor virus.indirect immunoferritin and fixed immunofluorescence tests were carried out on (a) sera of mice hyperimmunized with isologous mouse mammary tumor virus (mmtv) particles or isologous mmtv-producing mammary tumor cells grown in tissue culture and (b) sera of mammary tumor-bearing and tumor-free mice of several inbred strains. sera were tested against mmtv produced by c3h/hej/tex tissue culture cells (mmt-1). mammary tumor-bearing a/dm, c3h/hetex, and riii/dm mice and apparently tumor-free a/dm mic ...1977193633
organ distribution of exogenous murine mammary tumour virus as determined by bioassay. 1977193692
the role of t and suppressor cells in mtv-directed cellular immunity. 1977193694
[microsomal n-acetyl-glucosaminyl-transferases activity in balb/c mice infected with mouse mammary tumor virus (m-mtv) (author's transl)]. 1977193720
expression of thy 1 antigen in normal and neoplastic mammary cells of mice.the expression of thy 1.2 (theta c3h) antigen was measured on the membranes of normal and neoplastic riii and c3h mammary cells. competitive inhibition assays revealed that the average membrane content of thy 1.2 in mammary tissues was about equal to that of lymph node cells. higher percentages of thy 1.2-positive cells than mammary tumor virus (mumtv)-positive cells were observed by immunofluorescence, which suggested that not all the thy 1.2-positive cells recovered from tumors were also mumtv ...1977194040
bioactivity of c3h and riii mammary tumor viruses in virgin female balb/c mice.the bioactivities of c3h and riii mammary tumor virus (mumtv) in virgin female balb/c mice differed. the average number of mammary hyperplastic alveolar nodules per mouse after noduligenic tests was 20.1 in balb/cfc3h and 10.7 in balb/cfriii females. spontaneous mammary tumor incidence after 20 months of observation was 47.5% in balb/cfc3h and 14.6% in balb/cfriii females (p less than 0.01). the frequency of lung metastases in mammary tumor-bearing mice was 63.1% in balb/cfc3h and 16.6% in balb ...1977194047
role of h-2 histocompatibility on generation of cell-mediated cytotoxicity against virus-induced mammary tumors in c3h mice. 1977194369
differences in mouse mammary tumor viruses. relationship to early and late occurring mammary tumors.the murine model has been used extensively to study the various factors involved in the etiology of mammary carcinoma. inbred mouse strains have been classically categorized into (i) high incidence stains with tumors occurring relatively early in the life of the animal, or (ii) low or moderate incidence strains with tumors occurring later on in life. we have radioactively labeled the rna genome of the mouse mammary tumor virus (mmtv) from each of several mouse strains. we report here, using the ...1977194675
biology of breast preneoplasia.the literature dealing with mammary cancer and the presence of the identifiable preneoplastic lesions in the mammary gland of several species has been reviewed. a discrete morphologically identifiable lesion with a high malignant potential is considered preneoplastic. lobuloalveolar lesions have been identified in the human mammary gland which fit most of the criteria for preneoplasia. the lesions, hyperplastic atypical lobules, are multicentric, have a high statistical association with breast c ...1977194676
natural immunity to murine mammary tumor viruses. 1977194748
immunogenicity and mumtv-like antigenicity of human breast cancer tissues. 1977194749
an improved procedure for purifying rna tumor viruses from malignant tissue.a convenient method for purifying rna tumor viruses is described. viruses banded in isopycnic gradients are contaminated with membranes that can be removed by velocity sedimentation in glycerol gradients. on sodium dodecyl sulfate (sds) gel electrophoresis, the particles purified by these procedures from mouse mammary tumor tissue show protein profiles typical of those observed with virus purified from milk.1977214996
[glucocorticoid receptors in mammary tumors in ps and c3h mouse strains (author's transl)].specific binding of glucocorticoids was characterized in mammary tumors and cells derived from c3h and ps mice. the binding protein satisfied all the general criteria of hormone receptors namely high affinity and binding specificity for glucocorticoids (kd congruent to 2.5 nm) for 3h dexamethasone and limited number of binding sites (congruent to 150 femtomoles/mg protein). the hormonal regulation of these receptors will be reported. the production of viral particles of type a and b was increase ...1977206179
[carcinogenesis due to oncorna virus type c: biological, immunological and genetic aspects]. 1977205925
is there a role for actin in virus budding?electrophoretic data from both sodium dodecyl sulfate-polyacrylamide gels (sds-page) and acid-urea gels reveal a protein in purified murine mammary tumor virus (mumtv) which co-migrates with purified chick skeletal muscle actin. 125i-labeling of intact and disrupted virus preparations shows that the actin-like protein is not artifactually adsorbed to the outside of virions during isolation. quantitative sds-page and examination of negatively stained preparations show that the actin cannot be acc ...1977233748
purification and characterization of the dna polymerase of human breast cancer particles.previous studies have identified human breast tumor particles possessing many of the features characteristic of rna tumor viruses. in addition to the expected size (600 s) and density (1.16 g/ml) these include possession of an outer membrane and an inner one surrounding a "core" containing a dna polymerase and a large-molecular-weight (70s) rna possessing detectable homology to the rnas of the mouse mammary tumor virus (mmtv) and of the mason-pfizer monkey virus (mpmv). we report here the purifi ...1977265540
in vitro mouse mammary tumor virus transcription from chromatin. a system to study the mechanism of action of glucocorticoid hormones. 1977563804
gene order of the mouse mammary tumor virus glycoproteins.immunochemical and tryptic peptide mapping techniques were used to show that the mouse mammary tumor virus (mmtv) envelope glycoproteins gp52 and gp36 are distinct components derived from a common glycosylated precursor polypeptide of 75,000 daltons (gpr75). because both gp52 and gp36 are derived from a common precursor polypeptide and therefore have a common initiation site, we have been able to determine their gene order within the viral genome. the gene order was deduced from three different ...197718625476
leukocyte migration inhibition response to breast cancer antigens and to mouse mammary tumor virus in women with primary breast cancer. 1978233830
serological and biochemical characterization of the mouse mammary tumor virus with localization of p10. 1978205999
isolation of separate precursor polypeptides for the mouse mammary tumor virus glycoproteins and nonglycoproteins. 1978206000
eukaryotic gene regulation studies with mouse mammary tumor virus in tissue culture cells.the transcriptional regulation of mouse mammary tumor virus has been investigated in cell culture systems. these systems offer several insights into mechanisms of gene control including tenfold stimulation by glucocorticoids and spontaneous alterations in levels of constitutive transcription.1978219353
genetics of resistance of animals to viruses: i. introduction and studies in mice. 1978219668
studies on cell surface antigens of mouse leukemic and normal lymphocytes. ii. relationships between mammary leukemia, gross virus related and major histocompatibility antigens on l1210 leukemic lymphocytes.the presence of ml antigen on leukemia l1210 cells was confirmed by different procedures. in addition, by applying appropriate absorptions and blocking techniques combined with ferritin labelled antibodies in electron microscopy (em), the independent localization of ml antigen and murine leukemia virus gross (mulv-gross) cellular antigen on l1210 cells was demonstrated. the ferritin labeled antigenic sites appeared to be free from budding viral particles. in further studies the 3m kci extracts o ...1978219815
biological behaviour of two sublines of mammary cancer susceptible strains made free of mammary tumour virus (mtv) by foster-nursing. 1978232065
synthesis of mouse mammary tumor virus ribonucleic acid in isolated nuclei from cultured mammary tumor cells.glucocorticoid hormone treatment of gr cells, a cultured line derived from mouse mammary tumor tissue, selectively stimulates the rate of transcription of integrated proviral genes specifying mammary tumor virus (mtv). we have incubated isolated nuclei from these cells under conditions in which all three endogenous rna polymerases appear to be active. rna synthesized in vitro is distinguished from preexisting nuclear rna by labeling the in vitro products with [3h]ctp, and the level of mtv rna sy ...1978206279
radioimmunoassay for protein p28 of murine mammary tumor virus in organs and serum of mice and search for related antigens in human sera and breast cancer extracts.the main protein of the core of murine mammary tumor virus, with a molecular weight of 28,000 (p28), was solubilized by deoxycholate treatment of the virus and purified by ultrogel aca-54 filtration and hydroxyapatite chromatography. this protein was used as labeled antigen in a highly specific and reproducible radioimmunoassay. organ extracts of uninfected c57bl mice did not contain p28, but organ extracts of infected riii mice did contain the antigen. despite the high content in the mammary gl ...1978206358
leukocyte migration inhibition and lymphocyte blastogenesis responses in breast carcinoma patients to mouse mammary tumor virus and to virion gp52 antigen and rauscher murine leukemia virus-kirsten sarcoma virus gp69/71 antigen. 1978206704
effect of a mouse mammary tumor virus-derived protein vaccine on primary tumor development in mice.the vaccines used in this study were derived from purified murine mammary tumor virus (mumtv) preparations. approximately 60% of the protein fractions consisted of the major viral membrane glycoprotein gp52. inoculation sc of 10 microgram mumtv-s-derived vaccine significantly delayed the appearance of primary mammary tumors in gr and balb/cfc3h mice (strains with high incidences of mammary cancer); in balb/c and c3hf mice, which have a moderate tumor incidence at an advanced age, this treatment ...1978206711
genetic control of immune responses to moloney leukemia virus in rats.when bn and lew rats were immunized with untreated or with inactivated moloney murine leukemia virus (m-mulv), bn rats produced high antibody responses to the p15, p30, and gp70 antigens of the virus, whereas lew rats were low responders to these antigens. bn rats also exhibited a high response and lew rats a low response when the two strains were immunized with purified p30. studies of (lew x bn)f1 and backcross rats suggested that factors associated with agb exerted major influences on respons ...1978206712
production of unintegrated mouse mammary tumor virus dna in infected rat hepatoma cells is a secondary action of dexamethasone.dexamethasone, a synthetic glucocorticoid, selectively increased the rate of synthesis of mouse mammary tumor virus (mtv) rna in clonal isolates of chronically infected rat hepatoma tissue culture cells. this hormonal effect occurred extremely rapidly and appeared to be mediated directly by the glucocorticoid-specific receptor protein. in addition to the viral rna, unintegrated mtv dna was also detected in these cells. several lines of evidence are consistent with the idea that the unintegrated ...1978206732
detection of viral proteins in mouse mammary tumors by immunoperoxidase staining of paraffin sections.an indirect immunoperoxidase method is described, which can readily detect viral antigens in paraffin sections of primary, transplanted, and metastatic mammary tumors of mice. in addition to having the obvious advantage of not being limited to fresh specimens, immunoperoxidase staining of paraffin sections proved to be superior in many respects when compared with immunofluorescence and frozen sections. immunoperoxidase staining of paraffin sections is permanent and provides the kind of histologi ...1978206904
detection in human breast carcinomas of an antigen immunologically related to a group-specific antigen of mouse mammary tumor virus.an antigen immunologically related to a group-specific antigen (gp52, a 52,000-dalton glycoprotein) of the mouse mammary tumor virus has been identified in paraffin sections of human breast cancers by means of the indirect immunoperoxidase technique. the specificity of the reaction with antibody against mouse mammary tumor virus was examined by absorption of the igg with the following: (a) purified gp52; (b) a number of virus preparations (mouse mammary tumor virus, rauscher leukemia virus, simi ...1978206905
genetic analyses of differences in incidence of mammary tumors and reticulum cell neoplasms with the use of recombinant inbred lines of mice.the influence of genes, in addition to genes in the h-2 complex, that effect the genesis of mammary tumors was studied. the recombinant inbred (ri) cxb lines were chosen for this investigation, because they are well suited for the study of the genetics of a trait for which the genotype affects probability of phenotype expression and which therefore is measured as incidence. females of seven ri lines (cxbd, cxbe, cxbg, cxbh, cxbi, cxbj, and cxbk) and their progenitor strains c57bl/6by (b6) and ba ...1978212568
involvement of mouse mammary tumor virus in spontaneous and hormone-induced mammary tumors in low-mammary-tumor mouse strains.the involvement of the mouse mammary tumor virus (mtv) in spontaneous and hormone-induced mammary tumors in low-mammary-tumor mouse strains was studied by comparing the amounts of mtv rna and mtv dna sequences in mammary tumors and other tissues of mice with an without hormonal treatments. the following results were obtained. (i) mammary tumors which appeared in c3h mice as a result of an infection with mtv contained more mtv dna compared with noninfected organs; these mammary tumors also contai ...1978212580
strain-specific markers for the major structural proteins of highly oncogenic murine mammary tumor viruses by tryptic peptide analyses.tryptic peptide analyses were performed on the major structural 52,000- and 36,000-dalton glycoproteins (gp52 and gp36-38) and the nonglycosylated 28,000-, 14,000-, and 10,000-dalton proteins (p28, p14, and p10) of the highly oncogenic murine mammary tumor viruses (mmtvs) of c3h, riii, and gr mice, i.e., mmtv(c3h), mmtv(riii), and mmtv(gr), respectively. each virus was grown in both murine and feline cells to ensure the virus-coded nature of each peptide analyzed. the gp36-38 peptide maps of all ...1978212589
prospects of vaccines against cancer in man.preventive immunization is the cheapest and most effective approach to the management of epidemic disease. certain forms of cancer, including burkitt's lymphoma, carcinoma of the cervix, primary liver cancer, acute leukaemia and breast cancer show many characteristics of epidemic disease, and the possibility of producing preventive vaccine against these conditions merits serious consideration.1978212839
genetic control of endogenous murine mammary tumour viruses reinvestigated. 1978213287
occurrence of antibody against intracytoplasmic a-particles of mouse mammary tumor virus in sera from breast cancer patients.acetone-fixed smears of dba/2 mouse leukemia cells that produce clusters of intracytoplasmic a-particles (pronucleocapsids of mouse mammary tumor virus) were employed as an indirect immunofluorescence system to detect the antibody to a-particles in human sera. with positive test sera, specific fluorescence was easily detectable as discrete cytoplasmic granules at the site of a-particle clusters. the antibody was found in 26 (60%) out of 43 breast cancer patient sera and 4 (25%) of 16 mammary fib ...1978213337
murine mammary tumor virus (mumtv) infection of an epithelial cell line established from c57bl/6 mouse mammary glands. 1978213889
[virus and human cancers]. 1978214215
chemical carcinogenesis in c3h/stwi mice, a worthwhile experimental model for breast cancer. 1978214224
demonstration of mouse mammary tumour virus (mumtv) antigenicity in human milk by means of immunodiffusion technique.using the micro-ouchterlony immunodiffusion technique, we were able to demonstrate mouse mammary tumour virus (mumtv) antigenicity in the 1.26-1.28 g/ml density fraction prepared from pooled human milk samples after treatment with detergents and ether. the cross-reacting antigen(s) was precipitated by rabbit antisera to mumtv-b particles prepared from murine milk and intracytoplasmic a particles (iap) isolated from mouse mammary tumour tissue. by confluence of precipitin lines, the human milk "c ...1978214323
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