Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| relationship between body size and severity of dengue hemorrhagic fever among children aged 0-14 years. | a hospital based case-control study was conducted from october 2002 to november 2003 among children aged 0-14 years at queen sirikit national institute of child health (children's hospital), bangkok, thailand. this study focused on body size and severity of dengue hemorrhagic fever (dhf) in children. one hundred five patients diagnosed as having dhf grade iii or iv were the cases and 105 diagnosed as having dhf grade i or ii were controls. they were matched at a ratio of 1:1 by their gender and ... | 2006 | 17124987 |
| morphological studies in a model for dengue-2 virus infection in mice. | one of the main difficulties in studying dengue virus infection in humans and in developing a vaccine is the absence of a suitable animal model which develops the full spectrum of dengue fever, dengue haemorrhagic fever, and dengue shock syndrome. it is our proposal to present morphological aspects of an animal model which shows many similarities with the dengue infection in humans. balb/c mice were intraperitoneally infected with non-neuroadapted dengue virus serotype 2 (denv-2). histopathologi ... | 2006 | 17293987 |
| concurrent infection with dengue virus type-2 and denv-3 in a patient from ceará, brazil. | dengue outbreaks have occurred in several regions in brazil and cocirculating dengue virus type 1 (denv-1), denv-2, and denv-3 have been frequently observed. dual infection by denv-2 and denv-3 was identified by type-specific indirect immunofluorescence assay and confirmed by reverse transcription polymerase chain reaction in a patient in ceará with a mild disease. this is the first documented case of simultaneous infection with denv-2 and denv-3 in brazil. sequencing confirmed denv-2 and denv-3 ... | 2006 | 17293990 |
| crm1-dependent nuclear export of dengue virus type 2 ns5. | the dengue virus multidomain rna polymerase ns5 has been observed in the nucleus in mammalian infected cell systems. we previously showed that ns5 nuclear localization is mediated by two nuclear targeting signals within the ns5 interdomain region that are recognized by distinct members of the importin superfamily of intracellular transporters. intriguingly, we have recently found that ns5 also possesses the ability to be exported from the nucleus by the importin family member crm1 (exportin 1) b ... | 2006 | 17319160 |
| dengue in the americas and southeast asia: do they differ? | the populations of southeast asia (se asia) and tropical america are similar, and all four dengue viruses of asian origin are endemic in both regions. yet, during comparable 5-year periods, se asia experienced 1.16 million cases of dengue hemorrhagic fever (dhf), principally in children, whereas in the americas there were 2.8 million dengue fever (df) cases, principally in adults, and only 65,000 dhf cases. this review aims to explain these regional differences. in se asia, world war ii amplifie ... | 2006 | 17341332 |
| inhibitory potential of quercus lusitanica extract on dengue virus type 2 replication. | this study reports the in vitro inhibitory potential of crude extract of quercus lusitanica (q. lusitanica) seeds on the replication of dengue virus type 2 (den-2). in vitro antiviral activity of q. lusitanica extract, assessed in c6/36 cells (cloned cells of aedes albopictus larvae) employing a virus inhibition assay, showed dose-dependent inhibition. the q. lusitanica extract at its maximum non-toxic concentration of 0.25 mg/ml completely inhibited 10-1,000 tcid50 of virus, as indicated by the ... | 2006 | 17547068 |
| survival and replication of dengue-2 virus in diapausing eggs of aedes albopictus (diptera: culicidae). | survival and replication of dengue-2 virus (family flaviviridae, genus flavivirus, denv-2) was determined in diapausing eggs of aedes albopictus (skuse) (diptera: culicidae) to evaluate the importance of this mechanism for viral maintenance during adverse climatic conditions. infected and uninfected eggs of ae. albopictus were induced to diapause under low temperature and short photoperiod. virus was detected by reverse transcription-polymerase chain reaction and isolated in c6/36 cells from bot ... | 2007 | 17547236 |
| controlled peptide solvation in portion-mixing libraries of fret peptides: improved specificity determination for dengue 2 virus ns2b-ns3 protease and human cathepsin s. | the solubility of peptides in aqueous buffers used for the enzyme assays is a common limitation for all peptide libraries. in principle, the more water-soluble peptides are, the more susceptible they will be to peptidase hydrolysis. we have demonstrated that this bias can be circumvented in a portion-mixing fluorescence resonance energy transfer (fret) peptide library by introducing k (lysine in the d-form) in both termini of the peptides. this more solvated library and another one without the k ... | 2007 | 17563123 |
| interaction between the cellular protein eef1a and the 3'-terminal stem-loop of west nile virus genomic rna facilitates viral minus-strand rna synthesis. | rnase footprinting and nitrocellulose filter binding assays were previously used to map one major and two minor binding sites for the cell protein eef1a on the 3'(+) stem-loop (sl) rna of west nile virus (wnv) (3). base substitutions in the major eef1a binding site or adjacent areas of the 3'(+) sl were engineered into a wnv infectious clone. mutations that decreased, as well as ones that increased, eef1a binding in in vitro assays had a negative effect on viral growth. none of these mutations a ... | 2007 | 17626087 |
| characterization of retrovirus-based reporter viruses pseudotyped with the precursor membrane and envelope glycoproteins of four serotypes of dengue viruses. | in this study, we successfully established retrovirus-based reporter viruses pseudotyped with the precursor membrane and envelope (prm/e) proteins of each of the four serotypes of dengue viruses, which caused the most important arboviral diseases in this century. co-sedimentation of the dengue e protein and hiv-1 core proteins by sucrose gradient analysis of the pseudotype reporter virus of dengue virus type 2, d2(hivluc), and detection of hiv-1 core proteins by immunoprecipitation with anti-e m ... | 2007 | 17662331 |
| construction and characterization of a stable subgenomic dengue virus type 2 replicon system for antiviral compound and sirna testing. | self-replicating, non-infectious flavivirus subgenomic replicons have been broadly used in the studies of trans-complementation, adaptive mutation, viral assembly and packaging in kunjin, yellow fever and west nile viruses. we describe here the construction of subgenomic egfp- or renilla luciferase-reporter based dengue replicons of the type 2 new guinea c (ngc) strain and the establishment of stable bhk21 cell lines harboring the replicons. in replicon cells, viral proteins and rnas are stably ... | 2007 | 17662475 |
| analysis of secondary structure predictions of dengue virus type 2 ns2b/ns3 against crystal structure to evaluate the predictive power of the in silico methods. | multiple sequence alignment was performed against eight proteases from the flaviviridae family using clustalw to illustrate conserved domains. two sets of prediction approaches were applied and the results compared. firstly, secondary structure prediction was performed using available structure prediction servers. the second approach made use of the information on the secondary structures extracted from structure prediction servers, threading techniques and dssp database of some of the templates ... | 2007 | 17688447 |
| characterization of an antigenic site that contains a dominant, type-specific neutralization determinant on the envelope protein domain iii (ed3) of dengue 2 virus. | the surface of the mature dengue virus (denv) particle consists of 90 envelope (e) protein dimers that mediate both receptor binding and fusion. the e protein ectodomain can be divided into three structural domains designated ed1, ed2, and ed3, of which ed3 contains the critical and dominant virus-specific neutralization sites. in this study the ed3 epitopes recognized by seven, murine, igg1 denv-2 type-specific, monoclonal antibodies (mabs) were determined using site-directed mutagenesis of a r ... | 2007 | 17719070 |
| role of dendritic cells in differential susceptibility to viral demyelinating disease. | although persistent viral diseases are a global health concern, the mechanisms of differential susceptibility to such infections among individuals are unknown. here, we report that differential interactions between dendritic cells (dcs) and virus are critical in determining resistance versus susceptibility in the theiler murine encephalomyelitis virus-induced demyelinating disease model of multiple sclerosis. this virus induces a chronic demyelinating disease in susceptible mice, whereas the vir ... | 2007 | 17722981 |
| susceptibility of mouse macrophage j774 to dengue virus infection. | the aim of this study was to investigate whether the j774 mouse macrophage cell line could be used as an in vitro model for dengue virus infection (denv). after 3 days, infection in j774 cells was assessed by detecting dengue virus non-structural protein 1 (nsp-1) production either by dot blot or indirect immunofluorescence assay (ifa) of saponine-permeabilized j774 cells and then confirmed by rt-pcr (171 bp product, corresponding to the denv-2 core). based on the presence of nsp-1 in infected b ... | 2007 | 17356302 |
| panmicrobial oligonucleotide array for diagnosis of infectious diseases. | to facilitate rapid, unbiased, differential diagnosis of infectious diseases, we designed greenechippm, a panmicrobial microarray comprising 29,455 sixty-mer oligonucleotide probes for vertebrate viruses, bacteria, fungi, and parasites. methods for nucleic acid preparation, random primed pcr amplification, and labeling were optimized to allow the sensitivity required for application with nucleic acid extracted from clinical materials and cultured isolates. analysis of nasopharyngeal aspirates, b ... | 2007 | 17370518 |
| expression in pichia pastoris and immunological evaluation of a truncated dengue envelope protein. | among the dengue virus structural proteins, the envelope glycoprotein is the most important because of its antigenic characteristics. in this work, the e protein from dengue-2 virus truncated at the c-terminus region was successfully expressed in pichia pastoris. the e2trunc gene was cloned under the aox1 promoter from p. pastoris and the signal peptide of the sucrose invertase gene from saccharomyces cerevisiae. sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis of expression re ... | 2007 | 17401146 |
| attenuated dengue 2 viruses with deletions in capsid protein derived from an infectious full-length cdna clone. | a full-length cdna clone (pd212) of dengue virus type 2 isolated in china (den2-43) was constructed. based on this, we constructed several mutants with deletions in capsid protein c using fusion pcr. these deletions removed part or almost all of the internal stretch of hydrophobic amino acid residues that is probably involved in virion assembly. we thus obtained viable mutant viruses. the propagation capacity of the mutant viruses in cell culture was impaired in parallel with the increasing size ... | 2007 | 17412442 |
| dengue viruses can infect human primary lung epithelia as well as lung carcinoma cells, and can also induce the secretion of il-6 and rantes. | dengue viruses (denv) are herein demonstrated for the first time as being able to infect and replicate in human primary lung epithelium and various lung cancer cell lines. the detection of dengue virus particles and viral negative strand rna synthesis in the cell, in conjunction with the release of viral progenies in culture supernatants, support the notion that lung cells are susceptible to dengue virus infection. the replication efficiency of denv in lung cancer cells from high to low is: den- ... | 2007 | 17416433 |
| structural bases for substrate recognition and activity in meaban virus nucleoside-2'-o-methyltransferase. | viral methyltransferases are involved in the mrna capping process, resulting in the transfer of a methyl group from s-adenosyl-l-methionine to capped rna. two groups of methyltransferases (mtases) are known: (guanine-n7)-methyltransferases (n7mtases), adding a methyl group onto the n7 atom of guanine, and (nucleoside-2'-o-)-methyltransferases (2'omtases), adding a methyl group to a ribose hydroxyl. we have expressed and purified two constructs of meaban virus (mv; genus flavivirus) ns5 protein m ... | 2007 | 17473012 |
| neutralizing antibodies after infection with dengue 1 virus. | severity of disease is markedly increased when infection with dengue virus type 2 (denv-2) follows infection with denv-1 at an interval of 20 years. studies have shown that heterologous neutralizing antibody titers are inversely correlated with severity of a second infection. if this mechanism controlled disease severity in cuba, heterotypic antibody titers should have declined over time. to determine whether phenotypic changes in dengue antibodies occur over time, we analyzed serum samples coll ... | 2007 | 17479892 |
| combinatorial computational approaches to identify tetracycline derivatives as flavivirus inhibitors. | limited structural information of drug targets, cellular toxicity possessed by lead compounds, and large amounts of potential leads are the major issues facing the design-oriented approach of discovering new leads. in an attempt to tackle these issues, we have developed a process of virtual screening based on the observation that conformational rearrangements of the dengue virus envelope protein are essential for the mediation of viral entry into host cells via membrane fusion. screening was bas ... | 2007 | 17502914 |
| high-level expression of recombinant dengue virus type 2 envelope domain iii protein and induction of neutralizing antibodies in balb/c mice. | dengue fever is a growing public health problem in many countries since so far no effective vaccines are available. in this study, the domain iii of dengue virus type 2 envelope was expressed in escherichia coli without fusion of any carrier protein. the recombinant protein was detected in the form of inclusion bodies, which were solubilized in 8m urea and could be purified subsequently by high-performance liquid chromatography (hplc) on an ion exchange column. after refolding, the recombinant p ... | 2007 | 17532481 |
| nuclear localization of dengue virus nonstructural protein 5 through its importin alpha/beta-recognized nuclear localization sequences is integral to viral infection. | dengue virus nonstructural protein 5 (ns5) is a large multifunctional protein with a central role in viral replication. we previously identified two nuclear localization sequences (nlss) within the central region of dengue virus type-2 (denv-2) ns5 ('anls' and 'bnls') that are recognized by the importin alpha/beta and importin beta1 nuclear transporters, respectively. here, we demonstrate the importance of the kinetics of ns5 nuclear localization to virus production for the first time and show t ... | 2007 | 17537211 |
| glycosylation of the dengue 2 virus e protein at n67 is critical for virus growth in vitro but not for growth in intrathoracically inoculated aedes aegypti mosquitoes. | to determine the importance of dengue 2 virus (den2v) envelope (e) protein glycosylation, virus mutants in one or both of the n-linked glycosylation motifs were prepared. we found that while the e2 mutant virus (n153q) replicated in mammalian and mosquito cells, the e1 (n67q) and e1/2 (n67q and n153q) mutant viruses were unable to grow in mammalian cells. infection of c6/36 mosquito cells with either the e1 or e1/2 mutants resulted in the introduction of a compensatory mutation, k64n, restoring ... | 2007 | 17543367 |
| a fusogenic peptide expressed on the surface of salmonella enterica elicits ctl responses to a dengue virus epitope. | attenuated salmonella strains are used widely as live carriers of antigens because they elicit both mucosal and systemic immunity against passenger antigens. however, they generally evoke poor cytotoxic t cell (ctl) responses because salmonella resides within vacuolar compartments and the passenger antigens must travel to the cytosol and be processed through the mhc class i-dependent pathway to simulate ctls. to address this problem, we designed a fusion protein to destabilize the phagosome memb ... | 2007 | 17543427 |
| characterization of humoral and cellular immune responses in cynomolgus macaques upon primary and subsequent heterologous infections with dengue viruses. | since studying the pathogenesis of dengue virus associated disease in humans has several limitations, an appropriate animal model is needed. therefore, we investigated kinetics of viremia as well as humoral and cellular immune responses, after primary, secondary and tertiary heterologous dengue virus infections in cynomolgus macaques: these parameters were largely similar to those observed in natural human infection upon primary infection. both antibody and t-cell responses measured were largely ... | 2007 | 17544804 |
| antibody responses are generated to immunodominant elk/kle-type motifs on the nonstructural-1 glycoprotein during live dengue virus infections in mice and humans: implications for diagnosis, pathogenesis, and vaccine design. | antibodies generated to the purified dengue type 2 virus (d-2v) nonstructural-1 (ns1) protein in mice and rabbits were compared with those generated to this protein in congeneic (h-2 class ii) mouse strains and humans after d-2v infections. unlike the profiles observed with the rabbits, similar antibody reaction profiles were generated by mice and humans with severe d-2v disease (dengue hemorrhagic fever [dhf]/dengue shock syndrome [dss]). many of these epitopes contained the core acidic-hydroph ... | 2007 | 17329445 |
| interference in dengue virus adsorption and uncoating by carrageenans. | this study demonstrated that the lambda- and iota-carrageenans, sulfated polysaccharides containing linear chains of galactopyranosyl residues, are potent inhibitors of dengue virus type 2 (denv-2) and 3 (denv-3) multiplication in vero and hepg2 cells, with values of effective concentration 50% from 0.14 to 4.1 microg/ml. this activity was assayed by plaque reduction, virus yield inhibition and antigen expression tests, and was independent of the input multiplicity of infection in the range 0.00 ... | 2007 | 17337028 |
| a novel tetraspanin c189 upregulated in c6/36 mosquito cells following dengue 2 virus infection. | dengue (den) viruses cause apoptosis in mammalian cells, but usually result in high progeny yields without evident damage in mosquito cells. by using subtractive hybridization, 13 potentially virus-induced genes were selected in den-2 virus-infected aedes albopictus c6/36 cells. based on semi-quantitative and real-time rt-pcr, one novel gene, named c189, was significantly upregulated in infected c6/36 cells. its full-length of 678 nucleotides (nt) was determined by a combination of 5'- and 3'-ra ... | 2007 | 17156880 |
| a mouse cell-adapted ns4b mutation attenuates west nile virus rna synthesis. | an adaptive mutation (e249g) within west nile virus (wnv) ns4b gene was consistently recovered from replicon rnas in c3h/he mouse cells. the e249g is located at the c-terminal tail of ns4b predicted to be on the cytoplasmic side of the endoplasmic reticulum membrane. the e249g substitution reduced replicon rna synthesis. compared with the wild-type ns4b, the e249g mutant protein exhibited a similar efficiency in evasion of interferon-beta response. recombinant e249g virus exhibited smaller plaqu ... | 2007 | 17178141 |
| substrate specificity of recombinant dengue 2 virus ns2b-ns3 protease: influence of natural and unnatural basic amino acids on hydrolysis of synthetic fluorescent substrates. | a recombinant dengue 2 virus ns2b-ns3 protease (ns means non-structural virus protein) was compared with human furin for the capacity to process short peptide substrates corresponding to seven native substrate cleavage sites in the dengue viral polyprotein. using fluorescence resonance energy transfer peptides to measure kinetics, the processing of these substrates was found to be selective for the dengue protease. substrates containing two or three basic amino acids (arg or lys) in tandem were ... | 2007 | 17184724 |
| characterization of the west nile virus protease substrate specificity and inhibitors. | west nile virus (wnv), a mosquito-borne member of flaviviridae, is a human pathogen causing widespread disease for which there is no vaccine or chemotherapy. the two-component viral serine protease consists of a heterodimeric complex between the hydrophilic domain of the cofactor, ns2b (ns2bh) and the protease domain (ns3-pro). the protease is essential for polyprotein processing followed by assembly of viral replicase and genome replication. therefore, the protease is an excellent target for de ... | 2007 | 17188926 |
| the envelope glycoprotein domain iii of dengue virus serotypes 1 and 2 inhibit virus entry. | dengue virus (dv) is a flavivirus and its urban transmission is maintained largely by its mosquito vectors and vertebrate host, often human. in this study, investigation was carried out on the involvement of domain iii of the envelope (e) glycosylated protein of dengue virus serotypes 1 and 2 (dv-1 and dv-2 diii) in binding to host cell surfaces, thus mediating virus entry. domain iii protein of flavivirus can also serve as an attractive target in inhibiting virus entry. the respective dv diii p ... | 2007 | 17196419 |
| membrane topology of murine coronavirus replicase nonstructural protein 3. | mouse hepatitis virus (mhv) is a member of the family coronaviridae. these positive strand rna viruses encode a replicase polyprotein that is processed into 16 nonstructural proteins (nsps). the nsps assemble with membranes to generate double membrane vesicles, which are the sites of viral rna synthesis. mhv nsp3 contains multiple domains including two papain-like protease domains, plp1 and plp2, and a predicted transmembrane (tm) domain. in this study, we determined the membrane topology of nsp ... | 2007 | 17222884 |
| [preparation and identification of dengue virus with deletion mutation of capsid protein]. | to generate rescued viruses with deletion mutation of capsid protein from dengue virus type 2 isolated in china (den2-43). | 2007 | 17259139 |
| high-yield production of short gpppa- and 7megpppa-capped rnas and hplc-monitoring of methyltransfer reactions at the guanine-n7 and adenosine-2'o positions. | many eukaryotic and viral mrnas, in which the first transcribed nucleotide is an adenosine, are decorated with a cap-1 structure, (7me)g5'-ppp5'-a(2'ome). the positive-sense rna genomes of flaviviruses (dengue, west nile virus) for example show strict conservation of the adenosine. we set out to produce gpppa- and (7me)gpppa-capped rna oligonucleotides for non-radioactive mrna cap methyltransferase assays and, in perspective, for studies of enzyme specificity in relation to substrate length as w ... | 2007 | 17259217 |
| dengue virus type 2: replication and tropisms in orally infected aedes aegypti mosquitoes. | to be transmitted by its mosquito vector, dengue virus (denv) must infect midgut epithelial cells, replicate and disseminate into the hemocoel, and finally infect the salivary glands, which is essential for transmission. the extrinsic incubation period (eip) is very relevant epidemiologically and is the time required from the ingestion of virus until it can be transmitted to the next vertebrate host. the eip is conditioned by the kinetics and tropisms of virus replication in its vector. here we ... | 2007 | 17263893 |
| the type 2 dengue virus envelope protein interacts with small ubiquitin-like modifier-1 (sumo-1) conjugating enzyme 9 (ubc9). | dengue viruses are mosquito-borne flaviviruses and may cause the life-threatening dengue hemorrhagic fever and dengue shock syndrome. its envelope protein is responsible mainly for the virus attachment and entry to host cells. to identify the human cellular proteins interacting with the envelope protein of dengue virus serotype 2 inside host cells, we have performed a screening with the yeast-two-hybrid-based "functional yeast array". interestingly, the small ubiquitin-like modifier-1 conjugatin ... | 2007 | 17265167 |
| infection and dissemination of dengue virus type 2 in aedes aegypti, aedes albopictus, and aedes scutellaris from the torres strait, australia. | to determine their relative roles in transmission of dengue virus (denv) in the torres strait region of northern australia, we examined infection and dissemination of a sympatric strain of dengue virus type 2 (denv-2) in aedes scutellaris, ae. albopictus, and ae. aegypti. in experiments using membrane feeders for virus exposure, infection rates were 83% and 43% for ae. scutellaris and ae. aegypti, respectively. salivary gland infection rates for both species were 43%. in experiments using pledge ... | 2007 | 18240514 |
| cleavage preference distinguishes the two-component ns2b-ns3 serine proteinases of dengue and west nile viruses. | regulated proteolysis of the polyprotein precursor by the ns2b-ns3 protease is required for the propagation of infectious virions. unless the structural and functional parameters of ns2b-ns3 are precisely determined, an understanding of its functional role and the design of flaviviral inhibitors will be exceedingly difficult. our objectives were to define the substrate recognition pattern of the ns2b-ns3 protease of west nile and dengue virises (wnv and dv respectively). to accomplish our goals, ... | 2007 | 17067286 |
| gb virus b disrupts rig-i signaling by ns3/4a-mediated cleavage of the adaptor protein mavs. | understanding the mechanisms of hepatitis c virus (hcv) pathogenesis and persistence has been hampered by the lack of small, convenient animal models. gb virus b (gbv-b) is phylogenetically the closest related virus to hcv. it causes generally acute and occasionally chronic hepatitis in small primates and is used as a surrogate model for hcv. it is not known, however, whether gbv-b has evolved strategies to circumvent host innate defenses similar to those of hcv, a property that may contribute t ... | 2007 | 17093192 |
| a recombinant capsid protein from dengue-2 induces protection in mice against homologous virus. | in the present work, we study the immunogenicity and protective capacity of a recombinant capsid protein from dengue-2 virus. the capsid gene was cloned under the t5 phage promoter and expressed in escherichia coli. the recombinant protein was obtained mainly associated to the soluble fraction upon cellular disruption and exhibited a pattern of high aggregation, determined by gel filtration chromatography. the semipurified preparation was inoculated in mice and after three doses, no antiviral an ... | 2007 | 17097199 |
| potential of ancestral sylvatic dengue-2 viruses to re-emerge. | dengue viruses (denv) are the most important arboviral pathogens in tropical and subtropical regions throughout the world. denv transmission includes both a sylvatic, enzootic cycle between nonhuman primates and arboreal mosquitoes of the genus aedes, and an urban, endemic/epidemic cycle between aedes aegypti, a mosquito with larval development in peridomestic water containers, and human reservoir hosts. all 4 serotypes of endemic denv evolved independently from ancestral sylvatic viruses and ha ... | 2007 | 17014880 |
| dna vaccines against dengue virus based on the ns1 gene: the influence of different signal sequences on the protein expression and its correlation to the immune response elicited in mice. | we analyzed four dna vaccines based on denv-2 ns1: pcens1, encoding the c-terminal from e protein plus the ns1 region; pcens1anc, similar to pcens1 plus the n-terminal sequence from ns2a (anc); pctpans1, coding the t-pa signal sequence fused to ns1; and pctpans1anc, similar to pctpans1 plus the anc sequence. the ns1 was detected in lysates and culture supernatants from pctpans1-, pcens1- and pcens1anc-transfected cells and not in cells with pctpans1anc. only the pcens1anc leads the expression of ... | 2007 | 17020777 |
| an atpase activity associated with the rotavirus phosphoprotein nsp5. | interactions between nsp5 and nsp2 drive the formation of viroplasms, sites of genome replication and packaging in rotavirus-infected cells. the serine-threonine-rich nsp5 transitions between hypo- and hyper-phosphorylated isomers during the replication cycle. in this study, we determined that purified recombinant nsp5 has a mg2+-dependent atp-specific triphosphatase activity that generates free adp and pi (vmax of 19.33 fmol of product/min/pmol of enzyme). the atpase activity was correlated wit ... | 2007 | 17825341 |
| type- and subcomplex-specific neutralizing antibodies against domain iii of dengue virus type 2 envelope protein recognize adjacent epitopes. | neutralization of flaviviruses in vivo correlates with the development of an antibody response against the viral envelope (e) protein. previous studies demonstrated that monoclonal antibodies (mabs) against an epitope on the lateral ridge of domain iii (diii) of the west nile virus (wnv) e protein strongly protect against infection in animals. based on x-ray crystallography and sequence analysis, an analogous type-specific neutralizing epitope for individual serotypes of the related flavivirus d ... | 2007 | 17881453 |
| effects of blood and virus-infected blood on protein expression in the midgut of the dengue vector aedes albopictus. | although the mosquito midgut is the primary site of bloodmeal storage and the first line of defence against pathogenic infection, little is known about its proteic composition at a time when an increasing number of proteins are reported to impair viral infection. aedes albopictus skuse (diptera: culicidae) is an important vector of the dengue virus. we compared 2-dimensional protein profiles of the adult midgut in this species, taking into account bloodmeal status. the comparison of profiles fro ... | 2007 | 17897369 |
| antibody neutralization and viral virulence in recurring dengue virus type 2 outbreaks. | outbreaks involving dengue viruses (denv) of the same genotype occur in a cyclical pattern in malaysia. two cycles of outbreaks involving dengue virus type 2 (denv-2) of the same genotype occurred in the 1990s in the klang valley, malaysia. sera of patients from the first outbreak and sera of mice inoculated with virus from the same outbreak had poorer neutralization activity against virus of the second outbreak. conversely, patient sera from the second outbreak showed higher neutralization tite ... | 2007 | 17931106 |
| mitochondrial and bioenergetic dysfunction in human hepatic cells infected with dengue 2 virus. | dengue virus infection affects millions of people all over the world. although the clinical manifestations of dengue virus-induced diseases are known, the physiopathological mechanisms involved in deteriorating cellular function are not yet understood. in this study we evaluated for the first time the associations between dengue virus-induced cell death and mitochondrial function in hepg2, a human hepatoma cell line. dengue virus infection promoted changes in mitochondrial bioenergetics, such as ... | 2007 | 17964123 |
| secreted ns1 of dengue virus attaches to the surface of cells via interactions with heparan sulfate and chondroitin sulfate e. | dengue virus (denv) nonstructural protein-1 (ns1) is a secreted glycoprotein that is absent from viral particles but accumulates in the supernatant and on the plasma membrane of cells during infection. immune recognition of cell surface ns1 on endothelial cells has been hypothesized as a mechanism for the vascular leakage that occurs during severe denv infection. however, it has remained unclear how ns1 becomes associated with the plasma membrane, as it contains no membrane-spanning sequence mot ... | 2007 | 18052531 |
| multiple antigenic peptides as vaccine platform for the induction of humoral responses against dengue-2 virus. | dengue is an important agent of human disease for which no licensed vaccine is available to the public. we used multiple antigenic peptides (maps) as an antigen carrier for the development of subunit vaccines against dengue-2 virus (den-2). commercially available software (macvector 7.0) was used to identify potential antigenic b-cell epitopes of e-glycoprotein. a total of 60 balb/c mice were immunized with 12 recombinant den-2-specific maps and the humoral immune response was assessed by anti-d ... | 2007 | 18158738 |
| an in vitro model for dengue virus infection that exhibits human monocyte infection, multiple cytokine production and dexamethasone immunomodulation. | an important cytokine role in dengue fever pathogenesis has been described. these molecules can be associated with haemorrhagic manifestations, coagulation disorders, hypotension and shock, all symptoms implicated in vascular permeability and disease worsening conditions. several immunological diseases have been treated by cytokine modulation and dexamethasone is utilized clinically to treat pathologies with inflammatory and autoimmune etiologies. we established an in vitro model with human mono ... | 2007 | 18209938 |
| miniaturized pcr chips for nucleic acid amplification and analysis: latest advances and future trends. | the possibility of performing fast and small-volume nucleic acid amplification and analysis on a single chip has attracted great interest. devices based on this idea, referred to as micro total analysis, microfluidic analysis, or simply 'lab on a chip' systems, have witnessed steady advances over the last several years. here, we summarize recent research on chip substrates, surface treatments, pcr reaction volume and speed, architecture, approaches to eliminating cross-contamination and control ... | 2007 | 17576684 |
| dengue virus type 2 isolated from an imported dengue patient in japan: first isolation of dengue virus from nepal. | we report the isolation of dengue virus type 2 from a dengue patient returning to japan from nepal in october, 2004. this is the first isolate of dengue virus in nepal. according to nucleotide homology, the virus was closest to a dengue virus type 2 isolate from india. | 2008 | 18217869 |
| universal primers that amplify rna from all three flavivirus subgroups. | species within the flavivirus genus pose public health problems around the world. increasing cases of dengue and japanese encephalitis virus in asia, frequent outbreaks of yellow fever virus in africa and south america, and the ongoing spread of west nile virus throughout the americas, show the geographical burden of flavivirus diseases. flavivirus infections are often indistinct from and confused with other febrile illnesses. here we review the specificity of published primers, and describe a n ... | 2008 | 18218114 |
| rapid detection of dengue virus in serum using magnetic separation and fluorescence detection. | a magnetophoretic fluorescence sensor (mfs) has been developed to rapidly detect dengue virus in serum at a sensitivity that was approximately three orders of magnitude higher than conventional solid phase immunoassays. uv inactivated type 2 dengue virus was first reacted with a mixture of superparamagnetic and fluorescent microparticles functionalised with an anti-type 2 dengue virus monoclonal antibody in 10% fetal calf serum. the magnetic particles were separated from the serum based on their ... | 2008 | 18227947 |
| human t-cell leukemia virus type 1 blunts signaling by interferon alpha. | although many animal viruses block the interferon (ifn) signaling pathway, this issue has not been previously investigated in retrovirus-infected cells. for this purpose, an infectious molecular clone of human t-cell leukemia virus type 1 (htlv-1) was transfected into 293t or hela cells and was found to reduce interferon-stimulated response element (isre) reporter activity. this effect was independent of expression of the polymerase or envelope products and independent of the ability of tax to a ... | 2008 | 18234266 |
| monoclonal antibodies that bind to common epitopes on the dengue virus type 2 nonstructural-1 and envelope glycoproteins display weak neutralizing activity and differentiated responses to virulent strains: implications for pathogenesis and vaccines. | the abilities of monoclonal antibodies (mabs) that bind to defined sequential epitopes on the dengue virus (denv) nonstructural-1 (ns1) glycoproteins to cross-react with epitopes on the denv envelope (e) glycoproteins were investigated. in this study, some of these mabs cross-reacted with the denv type 2 (denv-2) e glycoprotein and with synthetic peptides representing x-ray crystallographically confirmed surface-exposed regions on this glycoprotein. mab 1g5.3 cross-reacted with the flavivirus-co ... | 2008 | 18160621 |
| evaluation of an ns1 antigen detection for diagnosis of acute dengue infection in patients with acute febrile illness. | diagnosis of dengue infection during the febrile stage has been challenging. we evaluated the accuracy of ns1 protein detection in diagnosing dengue infection in patients presenting with acute febrile illness in bangkok, thailand. of the 235 subjects presented with fever of unknown source within 5 days, 132 (56.2%) were male with the median age of 17.8 (range, 3-52) years. the median duration of fever was 4 (range, 1-5) days. one hundred seventy-one (72.8%) patients had dengue infection, of whic ... | 2008 | 18191361 |
| functional entry of dengue virus into aedes albopictus mosquito cells is dependent on clathrin-mediated endocytosis. | entry of dengue virus 2 (denv-2) into aedes albopictus mosquito c6/36 cells was analysed using biochemical and molecular inhibitors, together with confocal and electron microscopy observations. treatment with monodansylcadaverine, chlorpromazine, sucrose and ammonium chloride inhibited denv-2 virus yield and protein expression, whereas nystatin, a blocker of caveolae-mediated endocytosis, did not have any effect. using confocal microscopy, co-localization of denv-2 e glycoprotein and the marker ... | 2008 | 18198378 |
| histidine protonation and the activation of viral fusion proteins. | many viral fusion proteins only become activated under mildly acidic condition (ph 4.5-6.5) close to the pk(a) of histidine side-chain protonation. analysis of the sequences and structures of influenza ha (haemagglutinin) and flaviviral envelope glycoproteins has led to the identification of a number of histidine residues that are not only fully conserved themselves but have local environments that are also highly conserved [kampmann, mueller, mark, young and kobe (2006) structure 14, 1481-1487] ... | 2008 | 18208382 |
| reliable detection of st. louis encephalitis virus by rt-nested pcr. | st. louis encephalitis virus (slev) is a re-emerging arbovirus in south america, with reported cases in humans in argentina and brazil. this fact indicates that there is an urgent need to increase the current knowledge about this virus in order to control and prevent future cases. exhaustive epidemiological and laboratory investigation is required to ensure fast, accurate identification of the viral agent and allow prompt surveillance action by health authorities. herein, we report the developme ... | 2008 | 18208760 |
| larval competition alters susceptibility of adult aedes mosquitoes to dengue infection. | dengue, the most important human arboviral disease, is transmitted primarily by aedes aegypti and, to a lesser extent, by aedes albopictus. the current distributions of these invasive species overlap and are affected by interspecific larval competition in their container habitats. here we report that competition also enhances dengue infection and dissemination rates in one of these two vector species. we determined the effects of competition on adult a. aegypti and a. albopictus, comparing their ... | 2008 | 18077250 |
| primary and secondary infections of macaca fascicularis monkeys with asian and american genotypes of dengue virus 2. | the goal of this study was to compare the immune response and the protection capacity induced by the dengue virus 2 (denv-2) american and asian genotypes in macaca fascicularis monkeys. animals were infected with american or asian denv-2 strains and challenged 1 year later with a denv-2 asian genotype strain. the viremia and monkey antibody levels were similar for the different strains after primary and secondary infection; however, the functionality of the antibody response was different. a lim ... | 2008 | 18094112 |
| solution structure and neutralizing antibody binding studies of domain iii of the dengue-2 virus envelope protein. | 2008 | 18004779 | |
| novel anti-dengue monoclonal antibody recognizing conformational structure of the prm-e heterodimeric complex of dengue virus. | an interaction between the premembrane (prm) and envelope (e) glycoproteins as prm-e heterodimer is required for proper folding and transport of e during the formation and release of new flaviviral progeny. more evidence, however, is needed to confirm this interaction of prm and e during dengue virus replication. in this study, 2e11, a mouse monoclonal antibody (mab) that specifically recognizes dengue prm-e heterodimeric complex in either intracellular or secreted dengue virions, was generated ... | 2008 | 18041028 |
| distinct rig-i and mda5 signaling by rna viruses in innate immunity. | alpha/beta interferon immune defenses are essential for resistance to viruses and can be triggered through the actions of the cytoplasmic helicases retinoic acid-inducible gene i (rig-i) and melanoma differentiation-associated gene 5 (mda5). signaling by each is initiated by the recognition of viral products such as rna and occurs through downstream interaction with the ips-1 adaptor protein. we directly compared the innate immune signaling requirements of representative viruses of the flaviviri ... | 2008 | 17942531 |
| genome 3'-end repair in dengue virus type 2. | genomes of rna viruses encounter a continual threat from host cellular ribonucleases. therefore, viruses have evolved mechanisms to protect the integrity of their genomes. to study the mechanism of 3'-end repair in dengue virus-2 in mammalian cells, a series of 3'-end deletions in the genome were evaluated for virus replication by detection of viral antigen ns1 and by sequence analysis. limited deletions did not cause any delay in the detection of ns1 within 5 d. however, deletions of 7-10 nucle ... | 2008 | 18974278 |
| persistency of transovarial dengue virus in aedes aegypti (linn.). | a study was conducted to examine the persistency of transovarial dengue virus type 2 (den-2) in a selangor strain of aedes aegypti mosquitoes. two hundred 4-5 day old female mosquitoes were fed with blood containing dengue virus. the infected mosquitoes were reared to the 7th generation; each generation was screened for the virus using immunological staining methods. the virus was detectable until the 5th generation but absent in the 6th and the 7th generations. therefore, dengue virus type 2 ca ... | 2008 | 19058573 |
| production of a monoclonal antibody against non-structural protein 3 of dengue-2 virus by intrasplenic injection. | dengue fever and dengue hemorrhagic fever/dengue shock syndrome are highly infectious diseases caused by dengue virus (dv). dv non-structural protein 3 (ns3) is known to possess atpase, helicase, and protease activity that is a constitutive part of the replication complex of dv. in this study, we discuss the cloning, expression, and purification of the dv-2 ns3 protein to immunize mice by intrasplenic injection and then to generate a monoclonal antibody (mab). one mab, named 4f5, was obtained an ... | 2008 | 19108619 |
| [express characterization of dengue virus type 2 structural gene e]. | 2008 | 18956691 | |
| eating the enemy within: autophagy in infectious diseases. | autophagy is emerging as a central component of antimicrobial host defense against diverse viral, bacterial, and parasitic infections. in addition to pathogen degradation, autophagy has other functions during infection such as innate and adaptive immune activation. as an important host defense pathway, microbes have also evolved mechanisms to evade, subvert, or exploit autophagy. additionally, some fungal pathogens harness autophagy within their own cells to promote pathogenesis. this review wil ... | 2008 | 18772897 |
| predicting the sensitivity and specificity of published real-time pcr assays. | in recent years real-time pcr has become a leading technique for nucleic acid detection and quantification. these assays have the potential to greatly enhance efficiency in the clinical laboratory. choice of primer and probe sequences is critical for accurate diagnosis in the clinic, yet current primer/probe signature design strategies are limited, and signature evaluation methods are lacking. | 2008 | 18817537 |
| massively parallel pathogen identification using high-density microarrays. | identification of microbial pathogens in clinical specimens is still performed by phenotypic methods that are often slow and cumbersome, despite the availability of more comprehensive genotyping technologies. we present an approach based on whole-genome amplification and resequencing microarrays for unbiased pathogen detection. this 10 h process identifies a broad spectrum of bacterial and viral species and predicts antibiotic resistance and pathogenicity and virulence profiles. we successfully ... | 2008 | 21261824 |
| induction of neutralizing antibodies against dengue virus type 2 upon mucosal administration of a recombinant lactococcus lactis strain expressing envelope domain iii antigen. | mucosal vaccines present several advantages over conventional parenteral vaccines including their ease of administration and low cost, both criteria being priorities for developing countries plagued by infectious diseases. a recombinant lactococcus lactis strain producing the envelope domain iii (ediii) antigen from dengue virus serotype 2 was engineered, and the ability of the live recombinant bacteria to trigger a systemic anti-ediii igg antibody response upon nasal or oral administration to b ... | 2008 | 18243432 |
| a strong endoplasmic reticulum retention signal in the stem-anchor region of envelope glycoprotein of dengue virus type 2 affects the production of virus-like particles. | recombinant virus-like particles (vlps) of flaviviruses have been shown to be produced efficiently by co-expressing the precursor membrane (prm) and envelope (e) proteins with few exceptions, such as dengue virus type 2 (denv2). it was reported previously that chimeric denv2 prm/e construct containing the stem-anchor region of e protein of japanese encephalitis virus (jev) produced vlps efficiently (chang, g. j., hunt, a. r., holmes, d. a., springfield, t., chiueh, t. s., roehrig, j. t., and gub ... | 2008 | 18252258 |
| toward universal flavivirus identification by mass cataloging. | development of rapid and specific molecular diagnostics for flaviviruses remains an important global health challenge. herein a platform technology using mass spectrometry that can be used for universal identification and genotyping of these viruses is described. the feasibility of the approach is demonstrated by using it to correctly identify and serotype two strains of dengue virus. predictive calculations show that the approach can be expected to be equally efficacious for the identification ... | 2008 | 18258926 |
| asymmetric competitive suppression between strains of dengue virus. | within-host competition between strains of a vector-borne pathogen can affect strain frequencies in both the host and vector, thereby affecting viral population dynamics. however little is known about inter-strain competition in one of the most genetically diverse and epidemiologically important mosquito-borne rna virus: dengue virus (denv). to assess the strength and symmetry of intra-host competition among different strains of denv, the effect of mixed infection of two denv serotypes, denv2 an ... | 2008 | 18261207 |
| peripheral blood mononuclear cells increase the permeability of dengue virus-infected endothelial cells in association with downregulation of vascular endothelial cadherin. | plasma leakage is one of the characteristic features of dengue haemorrhagic fever. the interaction among peripheral blood mononuclear cells (pbmcs), dengue virus and endothelial cells was analysed in vitro. human umbilical vein endothelial cells (huvecs) were infected with dengue-2 virus (dv-2) at an m.o.i. of 0.5 p.f.u. per cell. pbmcs were added to dv-2-infected huvecs, and transendothelial electrical resistance (teer) and transalbumin permeability were assessed. dengue virus infection at an m ... | 2008 | 18272754 |
| immunomodulating and antiviral activities of uncaria tomentosa on human monocytes infected with dengue virus-2. | uncaria tomentosa (willd.) dc., a large woody vine native to the amazon and central american rainforests has been used medicinally by indigenous peoples since ancient times and has scientifically proven immunomodulating, anti-inflammatory, cytotoxic and antioxidant activities. several inflammatory mediators that are implicated in vascular permeability and shock are produced after dengue virus (denv) infection by monocytes, the primary targets for virus replication. here we assessed the immunoreg ... | 2008 | 18279801 |
| sylvatic dengue virus type 2 activity in humans, nigeria, 1966. | using phylogenetic analysis of complete virus genomes from human isolates obtained in nigeria in 1966, we identified sylvatic dengue virus (denv) strains from 3 febrile patients. this finding extends current understanding of the role of sylvatic denv in febrile disease and documents another focus of sylvatic denv transmission in west africa. | 2008 | 18325274 |
| genetic relationships among aedes aegypti collections in venezuela as determined by mitochondrial dna variation and nuclear single nucleotide polymorphisms. | a population genetic analysis of gene flow was conducted among 619 aedes aegypti from nine collections distributed among six geographic regions of venezuela. genetic markers included a 387-basepair region of the mitochondrial nadh dehydrogenase 4 (nd4) gene and single nucleotide polymorphisms (snps) at 11 nuclear loci. genotypes at snp loci were identified using melting curve analysis. six different nd4 haplotypes were detected and patterns of variation suggested that collections were isolated b ... | 2008 | 18337347 |
| the relative contribution of antibody and cd8+ t cells to vaccine immunity against west nile encephalitis virus. | west nile virus (wnv) is a mosquito borne, neurotropic flavivirus that causes a severe central nervous system (cns) infection in humans and animals. although commercial vaccines are available for horses, none is currently approved for human use. in this study, we evaluated the efficacy and mechanism of immune protection of two candidate wnv vaccines in mice. a formalin-inactivated wnv vaccine induced higher levels of specific and neutralizing antibodies compared to a dna plasmid vaccine that pro ... | 2008 | 18339459 |
| autophagic machinery activated by dengue virus enhances virus replication. | autophagy is a cellular response against stresses which include the infection of viruses and bacteria. we unravel that dengue virus-2 (dv2) can trigger autophagic process in various infected cell lines demonstrated by gfp-lc3 dot formation and increased lc3-ii formation. autophagosome formation was also observed under the transmission electron microscope. dv2-induced autophagy further enhances the titers of extracellular and intracellular viruses indicating that autophagy can promote viral repli ... | 2008 | 18353420 |
| the flavivirus precursor membrane-envelope protein complex: structure and maturation. | many viruses go through a maturation step in the final stages of assembly before being transmitted to another host. the maturation process of flaviviruses is directed by the proteolytic cleavage of the precursor membrane protein (prm), turning inert virus into infectious particles. we have determined the 2.2 angstrom resolution crystal structure of a recombinant protein in which the dengue virus prm is linked to the envelope glycoprotein e. the structure represents the prm-e heterodimer and fits ... | 2008 | 18369147 |
| structure of the immature dengue virus at low ph primes proteolytic maturation. | intracellular cleavage of immature flaviviruses is a critical step in assembly that generates the membrane fusion potential of the e glycoprotein. with cryo-electron microscopy we show that the immature dengue particles undergo a reversible conformational change at low ph that renders them accessible to furin cleavage. at a ph of 6.0, the e proteins are arranged in a herringbone pattern with the pr peptides docked onto the fusion loops, a configuration similar to that of the mature virion. after ... | 2008 | 18369148 |
| dengue 2 virus inhibits in vitro megakaryocytic colony formation and induces apoptosis in thrombopoietin-inducible megakaryocytic differentiation from cord blood cd34+ cells. | thrombocytopenia is frequently associated with dengue virus infection in humans. although antiplatelet immunopathogenic processes have been implicated in the origin of dengue-associated thrombocytopenia, the effect of dengue viruses on megakaryocyte differentiation remains incompletely understood. in this study, we examined the effect of human dengue 2 virus isolates on the in vitro growth and differentiation of thrombopoietin-induced megakaryopoiesis of cord blood cd34+ cells. dengue 2 viruses, ... | 2008 | 18371071 |
| vector competence of aedes aegypti populations from senegal for sylvatic and epidemic dengue 2 virus isolated in west africa. | vector competence of aedes aegypti populations from six locations in different bioclimatic zones and habitats of senegal was assessed for sylvatic (ard 140875) and epidemic (ara 6894) dengue 2 virus (denv-2) isolates. f1 generation mosquitoes were fed with a blood meal containing 10(6.5) and 1.6 x 10(7) tissue culture infectious dose (tcid50) of the sylvatic denv-2 isolate or 10(6.5) tcid50 of the epidemic isolate and incubated for 14 days. after this period, legs and wings of each individual we ... | 2008 | 18378270 |
| complete genome sequence analysis of dengue virus type 2 isolated in brunei. | in a previous study, we have reported the detection and isolation of dengue virus in brunei (osman, o., fong, m.y., devi, s., 2007. a preliminary study of dengue infection in brunei. jjid 60 (4), 205-208). den-2 was the predominant serotype followed by den-1. the full genomic sequences of 3 den-2 viruses isolated during the 2005-2006 dengue incident in brunei were determined. twenty-five primer sets were designed to amplify contiguous overlapping fragments of approximately 500-600 base pairs spa ... | 2008 | 18406488 |
| antibodies to envelope glycoprotein of dengue virus during the natural course of infection are predominantly cross-reactive and recognize epitopes containing highly conserved residues at the fusion loop of domain ii. | the antibody response to the envelope (e) glycoprotein of dengue virus (denv) is known to play a critical role in both protection from and enhancement of disease, especially after primary infection. however, the relative amounts of homologous and heterologous anti-e antibodies and their epitopes remain unclear. in this study, we examined the antibody responses to e protein as well as to precursor membrane (prm), capsid, and nonstructural protein 1 (ns1) of four serotypes of denv by western blot ... | 2008 | 18448542 |
| imaging the interaction between dengue 2 virus and human blood platelets using atomic force and electron microscopy. | thrombocytopenia is frequently associated with dengue virus infection. host factors such as anti-platelet immunopathogenic processes have been implicated in the origin of dengue-associated thrombocytopenia but the role of dengue virus in directly interacting with platelets and altering their hemostatic property remains incompletely understood. in the present study, we examined the effect of dengue 2 virus on the morphology and physiological activation profile of normal human platelets using atom ... | 2008 | 18467742 |
| mutagenesis of the dengue virus type 2 ns5 methyltransferase domain. | the flavivirus ns5 protein possesses both (guanine-n7)-methyltransferase and nucleoside-2'-o methyltransferase activities required for sequential methylation of the cap structure present at the 5' end of the flavivirus rna genome. seventeen mutations were introduced into the dengue virus type 2 ns5 methyltransferase domain, targeting amino acids either predicted to be directly involved in s-adenosyl-l-methionine binding or important for ns5 conformation and/or charged interactions. the effects o ... | 2008 | 18469001 |
| identification of human hnrnp c1/c2 as a dengue virus ns1-interacting protein. | dengue virus nonstructural protein 1 (ns1) is a key glycoprotein involved in the production of infectious virus and the pathogenesis of dengue diseases. very little is known how ns1 interacts with host cellular proteins and functions in dengue virus-infected cells. this study aimed at identifying ns1-interacting host cellular proteins in dengue virus-infected cells by employing co-immunoprecipitation, two-dimensional gel electrophoresis, and mass spectrometry. using lysates of dengue virus-infec ... | 2008 | 18471994 |
| west nile virus infection of drosophila melanogaster induces a protective rnai response. | to determine if west nile virus (wnv) infection of insect cells induces a protective rnai response, drosophila melanogaster s2 and aedes albopictus c6/36 cells were infected with wnv, and the production of wnv-homologous small rnas was assayed as an indicator of rnai induction. a distinct population of approximately 25 nt wnv-homologous small rnas was detected in infected s2 cells but not c6/36 cells. rnai knockdown of argonaute 2 in s2 cells resulted in slightly increased susceptibility to wnv ... | 2008 | 18501400 |
| use of synthetic peptides to represent surface-exposed epitopes defined by neutralizing dengue complex- and flavivirus group-reactive monoclonal antibodies on the native dengue type-2 virus envelope glycoprotein. | the reactions of neutralizing monoclonal antibodies (mabs) that defined dengue virus (denv) complex, flavivirus subgroup or group neutralizing epitopes were tested against synthetic peptide sequences from domains i, ii and iii of the envelope (e) glycoproteins of different denv-2 genotypes/strains. the denv complex-reactive mab identified the surface-exposed 304-gkfkv/ivkeia-313 peptides and the denv complex-conserved 393-kkgssigq/km-401 peptides in domain iii, which were located adjacently in t ... | 2008 | 18559931 |
| characterization of dengue virus complex-specific neutralizing epitopes on envelope protein domain iii of dengue 2 virus. | the surface of the mature dengue virus (denv) particle is covered with 180 envelope (e) proteins arranged as homodimers that lie relatively flat on the virion surface. each monomer consists of three domains (ed1, ed2, and ed3), of which ed3 contains the critical neutralization determinant(s). in this study, a large panel of denv-2 recombinant ed3 mutant proteins was used to physically and biologically map the epitopes of five denv complex-specific monoclonal antibodies (mabs). all five mabs reco ... | 2008 | 18562544 |
| sequence analysis of e/ns1 gene junction of dengue virus type 2 isolated in brunei. | a preliminary study of dengue infection in brunei between 2005 and 2006 showed that dengue 2 was the predominant serotype. a total of five den-2 isolates were isolated and maintained in the mosquito cell-line, albopictus c6/36. the sequence spanning the envelope and non-structural protein 1 (e/ns1) junction (positions 2311 to 2550) of the isolates were determined and analysed at the amino acid and nucleotide levels. alignment of the 240 nucleotide sequences among the five isolates showed changes ... | 2008 | 18567445 |
| treatment of ag129 mice with antisense morpholino oligomers increases survival time following challenge with dengue 2 virus. | to determine the antiviral activity of phosphorodiamidate morpholino oligomers (pmo) and peptide-conjugated pmo (ppmo) in ag129 mice infected with dengue 2 virus (denv-2). | 2008 | 18567576 |
| genetic and phenotypic characterization of sylvatic dengue virus type 2 strains. | the four serotypes of endemic dengue viruses (denv) circulate between humans and peridomestic aedes mosquitoes. at present endemic denv infect 100 million people per year, and a third of the global population is at risk. in contrast, sylvatic denv strains are maintained in a transmission cycle between nonhuman primates and sylvatic aedes species, and are evolutionarily and ecologically distinct from endemic denv strains. phylogenetic analyses place sylvatic strains basal to each of the endemic s ... | 2008 | 18570968 |