Publications

escherichia coli in gastroenteritis of children in auckland, new zealand.	a study of stool escherichia coli in 60 children with gastroenteritis and 18 control children was carried out in auckland, new zealand in 1977. toxigenic strains, heat labile and heat stable, predominated in the stools of only three children, all of whom had concomitant rotavirus. classical enteropathogenic e. coli (epec) were found in patients and controls. only one patient had many epec in the stool (086.h2), they were variably toxigenic and rotavirus was also present. no toxigenic serotype wa ...	1981	7031126
traveler's diarrhea associated with rotavirus infection: analysis of virus-specific immunoglobulin classes.	an enzyme-linked immunosorbent assay for the detection of rotavirus-specific immunoglobulin g (igg), igm, and iga antibodies was used in a serological study of traveler's diarrhea. the antigenically related simian rotavirus (sa-11) was used as antigen in this study. serum was obtained from two groups of volunteers before travel (pre-travel) and at various times after arrival at their destination (post-travel). one group consisted of 47 american peace corps volunteers who traveled to honduras, an ...	1981	6260664
clinical manifestations of diarrhea in calves infected with rotavirus and enterotoxigenic escherichia coli.	the susceptibility of gnotobiotic, colostrum-derived, or suckling calves to four bovine rotavirus isolates was found to be age dependent. calves older than 7 days remained clinically normal, although they excreted virus in their feces and subsequently developed antibody against the virus, enterotoxigenic escherichia coli, fed to gnotobiotic, colostrum-deprived, or suckling calves ranging in age from a few hours to 26 days old, only caused diarrhea in animals younger than 24 h old. in contrast, d ...	1981	6265493
glucose vs sucrose in oral rehydration solutions for infants and young children with rotavirus-associated diarrhea.	the use of oral rehydration solutions containing essential electrolytes and either glucose or sucrose of equal osmolality was compared in a double-blind sequential trial of 784 children with rotavirus-associated diarrhea treated at a center in rural bangladesh. the oral fluid failure rate was 11.5% for the sucrose-containing solution group and 7.3% for the glucose-containing group (p = ns). vomiting was a significantly more common cause of failure for the group treated with sucrose-containing or ...	1981	6264376
[rotaviruses: an agent of infantile diarrhea].		1981	6291426
[gastroenteritis caused by rotavirus. an epidemiologic survey in lyons. diagnostic value of the elisa method].		1981	6264380
neonatal bovine enteritis: detection of rotavirus by counterimmunoelectrophoresis and enzyme-linked immunosorbent assay.	counterimmunoelectrophoresis and enzyme-linked immunosorbent assay specific for rotavirus were performed on 253 calf gut and fecal specimens from eastern north dakota. rotavirus antigen was detected in 29 specimens by counterimmunoelectrophoresis and in 52 specimens by enzyme-linked immunosorbent assay. all of the counterimmunoelectrophoresis-positive specimens were positive by enzyme-linked immunosorbent assay. the counterimmunoelectrophoresis and the enzyme-linked immunosorbent assay, using a ...	1981	6266620
poly(a) polymerase activity in human rotavirus.	a poly(a) polymerase enzymic activity was found in partially purified preparations of a human rotavirus. the activity was demonstrated using conditions similar to those utilized for the detection of the poly(a) polymerase previously described in reovirus (incubation at 43 degrees c in 70 mm-tris buffer ph 7.5 containing 12 mm-mn2+). the enzymic activity was associated only with complete, double-shelled particles. characterization of the poly(a)-containing product of the in vitro reaction by gel ...	1981	6267183
identification of rotavirus particle types.	negative-contrast electron microscopy of purified rotavirus particles reveals two particle types: single-shelled and double-shelled particles. the relationship of these particle types, seen by negative staining, to the enveloped and various types of nonenveloped particles seen in thin sections of virus-infected cells was determined. thin-section and negative-contrast electron microscopic analyses were performed on cell lysates from simian rotavirus. sa11-infected cells and on highly purified dou ...	1981	6276323
gene-coding assignments of rotavirus double-stranded rna segments 10 and 11.	the gene-coding assignments for genome segments 10 and 11 of a simian virus and two human rotaviruses were determined. for those viruses having a "long" rna gel pattern (electropherotype), segments 10 and 11 encoded proteins ns(3) and o(4), respectively. the human virus with a "short" electropherotype had the opposite assignments and also differed in (enzyme-linked immunosorbent assay) serotype from the human virus with a long electropherotype.	1981	6264154
[personal experience with the application of the electron microscope in research on rotavirus in the feces of children with acute gastroenteritis].		1981	6291467
exanthem subitum not associated with rotavirus.		1981	6264296
an outbreak of rotavirus diarrhea among a nonimmune, isolated south american indian community.	during july-august 1977, an outbreak of acute diarrhea occurred in an unusually isolated population, the tiriyó indians, who live in the north of pará, brazil, near the border with surinam. diarrhea was reported by 157 (70%) of the 224 indians living in the village during the epidemic. there was one fatal case in a one year old child. rotavirus was detected by electron microscopy in one fecal specimen collected from an acute case of diarrhea. seroconversions were noted in 127 out of 168 (75.6%) ...	1981	6263087
comparison of direct electron microscopy, immune electron microscopy, and rotavirus enzyme-linked immunosorbent assay for detection of gastroenteritis viruses in children.	an approximate 10% suspension in water of the first available stool sample from 411 infants and young children with acute gastroenteritis was examined by electron microscopy (em) after 2 min of negative staining. this procedure enabled the detection of 88% of the 199 rotavirus infections, all of the 22 adenovirus infections, and 47% of the 15 approximately 27-nm virus infections ultimately detected by a combination of techniques, including immune electron microscopy (iem) and rotavirus enzyme-li ...	1981	6263947
development of serum and intestinal antibody response to rotavirus after naturally acquired rotavirus infection in man.	the temporal characteristics of the response of rotavirus specific igm, igg, iga in serum and secretory antibody in feces to rotavirus were studied in 77 hospitalized patients with rotavirus induced gastroenteritis. the response in serum was characterized by the sequential appearance of rotavirus specific igm, igg, and iga antibody. the igm antibody appeared to be higher in the acute phase of the disease and was subsequently replaced by the igg and iga antibodies. however, the titers of igg rota ...	1981	6276504
frequency of preclumped virus in routine fecal specimens from patients with acute nonbacterial gastroenteritis.	a low-speed centrifugation technique for the preparation of grids after minimal purification of fecal extracts is described for examination of viruses by direct electron microscopy using negative staining. results showed that adenovirus, astrovirus, rotavirus, and "small round" viruses were frequently shed into the gastrointestinal tract in clumps of variable size. differential centrifugation study showed that a substantial proportion of the virus in the sample was lost in the initial pellet at ...	1981	6263948
[incidence of rotavirus infection and in combined infection of rotavirus and enteropathogenic escherichia coli in french calves (author's transl)].	detection of a rotavirus in faeces of 789 calves developing diarrhoea gave positive results among 48% of the calves. the same investigation extended to 96 apparently healthy animals shows that 12.5% of the faeces contained rotavirus. it appears that all the last ones cannot be considered as healthy controls. association of rotavirus and e. coli k99+ is found in 5% of sick animals less than 10 days old.	1981	6284012
igg1 antibody in milk protects lambs against rotavirus diarrhoea.	newborn gnotobiotic lambs were fed a diet of diluted evaporated milk supplemented either with normal ewes' milk or with milk obtained from ewes injected parenterally during gestation with rotavirus. lambs fed 150 ml per day of milk collected 5 days after lambing from normal ewes were susceptible to rotavirus infection and diarrhoea, while lambs fed milk from vaccinated ewes collected either 5 or 12 days after lambing were protected. analysis of the milk by column chromatography showed the anti-r ...	1981	6283719
pathomorphology of the intestinal mucosa in diarrheic calves.		1981	6283853
extraction of rotavirus from human feces by treatment with lithium dodecyl sulfate.	a procedure has been developed for the isolation of rotavirus from human fecal specimens based on the resistance of the virus to treatment with cold 1% lithium dodecyl sulfate at neutral ph. a single detergent treatment of fecal material followed by low- and high-speed centrifugations yielded a virus suspension of sufficient purity for viral ribonucleic acid to be analyzed directly by electrophoresis on polyacrylamide gels.	1981	6261684
[further studies on the etiology of autumnal infantile gastroenteritis (author's transl)].		1981	6282404
[world epidemiological situation: viral disease incidence (author's transl)].	the importance of viral disease, although estimated with difficulty, seems very large in human pathology. viruses appear to be involved in most of respiratory disease. moreover, in tropical countries, measles and viral diarrhoeas (mainly rotaviruses) are fatal for many children. but smallpox eradication must be considered as the most important fact which occurred in the field during the last five years, and the consequences of this new situation are discussed. finally, beyond the recent question ...	1981	6282477
quantitative aspects of rotavirus excretion in childhood diarrhoea.	the amount of rotavirus (antigen) in diarrhoeal stools was determined from serially diluted faecal specimens using a solid phase radioimmunoassay (ria) method. the acute stage ria titres for rotavirus antigen ranged from 1:200 to 1:200 000 with a mean titre of 1:2 936 in a group of 16 infants with age range 2 to 14 months and 1:4 703 in a group of 12 children aged 25 to 94 months. in the younger age-group the diarrhoea lasted longer (mean 7.8 days) than in the older children (mean 5.4 days) desp ...	1981	6275655
etiology of childhood diarrhea and oral rehydration therapy in northeastern brazil.	this study was conducted from january 1977 to june 1978 in fortaleza, brazil, to evaluate the oral rehydration treatment recommended by the world health organization for children admitted with acute diarrhea; admission peaked in january-march of both years and children treated came from the lowest socioeconomic strata of the popultion. initial treatment consisted of intravenous administration of normal saline or 5% glucose with saline solution; intravenous therapy was continued until objective ...	1981	6275933
lymphocyte transformation to specific antigens associated with crohn's disease.	specific lymphocyte transformation to three micro-organisms that have been implicated in crohn's disease was performed. these were pseudomonas maltophilia, mycobacterium kansasii and rotavirus. no significant differences were observed for any of the antigens tested. simultaneous testing of nonspecific stimulation by pha also showed no difference between patients and controls. these results do not support an aetiological role for these organisms mediated by a purely cellular mechanism.	1981	6284623
rotavirus infections associated with diarrhoea in calves in egypt.	the successful isolation and identification of rotavirus from newborn calves with diarrhoea is reported for the first time in egypt. from 25 faecal samples taken from diarrhoeic calves, ten virus isolates were found to give cytopathogenic effects on bovine embryonic kidney cells. three of the isolates were identified as rotavirus using fluorescent antibody staining, serum-neutralization, revealed the presence of rotavirus antibodies in 18 of 105 serum samples obtained from other calves slaughter ...	1981	6285588
[an outbreak of rotavirus gastroenteritis in a home for the elderly (author's transl)].		1981	6285841
diarrhoea in foals.		1981	6286504
routine isolation and cultivation of bovine rotaviruses in cell culture.	using the bovine embryonic kidney cell line, aubek, bovine rotaviruses were routinely isolated from fecal samples of calves with diarrhea. of 125 fecal samples positive for rotavirus by immune electron microscopy and the enzyme-linked immunosorbent assay, 61 isolates were recovered and cultivated continuously.	1981	6280522
[rotaviruses as causal agents of acute gastroenteritis. virological and serological studies in samples of the population of palermo].		1981	6280624
[demonstration of rotaviruses in feces by means of the complement fixation test].	complement fixation test for the detection of rotavirus in stools has been evaluated. removal of anticomplementary activity from the stools by absorption with complement or fetal calf serum was obtained. then human reference serum pools were checked for the optimal dilution to be employed in the test. sensibility and specificity of the c.f. method was determined on faecal specimens controlled by em. the results indicate that c.f. test is adequate for, and easily adaptable to the detection of rot ...	1981	6280738
a survey of zoo mammals for antibody to rotavirus.	the prevalence of rotavirus antibody in zoo animals was examined. a wide range of animals were found to be seropositive, indicating a widespread susceptibility to rotavirus infection.	1981	6280818
protein synthesis in cells infected with bovine rotavirus.	bovine rotavirus was found to multiply efficiently in llc-mk2 cells, a continuous line of rhesus monkey kidney, with a growth cycle which was essentially completed within 9 h after infection. the presence of low concentrations of trypsin (10 microgram/ml) in the virus inoculum was essential for infectivity. polyacrylamide gel electrophoresis of infected cell extracts demonstrated the synthesis of at least eight virus-specific polypeptides 6 h post-infection with mol. wt. ranging from 102 x 10(3) ...	1981	6267184
structural polypeptides of simian rotavirus sa11 and the effect of trypsin.	analysis of purified simian rotavirus has shown that it contains fewer structural polypeptide classes than previously reported. two polypeptides (molecular weights, 62,000 and 28,000) commonly found in purified rotaviruses were, in fact, produced by cleavage of a larger structural polypeptide (molecular weight, about 88,000) by trypsin, which is usually employed to increase the yield of rotaviruses in tissue culture. trypsin-uncleaved, double-shelled rotaviruses are probably composed of only fiv ...	1981	6260970
virus diarrhoea associated with pale fatty faeces.	steatorrhoea was a significant feature in an outbreak of rotavirus gastroenteritis which affected adults and infants in hospital. fat globules or fatty acid crystals were obvious by light microscopy (lm) in faeces from 14 of 25 patients examined. ten of the fatty stools and two of the remainder were very pale. by electron microscopy (em) a rotavirus was seen in 11 of the 14 fatty faeces and in only two of 11 specimens without visible fat. in a further study of pale or fatty faeces 20 such specim ...	1981	6270209
a survey of rotavirus infection in the topics.	the age distribution of rotavirus antibody in the serum of inhabitants of laos and indonesia was studied by the neutralization test using antigenically related calf rotavirus (ncdv) as a substitute for non-cultivable human rotavirus. the results revealed that both the rate of antibody-positives and the modal titers of antibody distribution by each age group in these countries were higher than those in respective are groups of japanese, which suggest higher endemicity of rotavirus infection in th ...	1981	6276600
light microscopy and ultrahistology of intestinal changes in pigs infected with epizootic diarrhoea virus (evd): comparison with transmissible gastroenteritis (tge) virus and porcine rotavirus infections.		1981	6277107
[rotavirus gastroenteritis].		1981	6277112
incidence of rotavirus, adenovirus and herpesvirus infection in pigeons.		1981	6278694
[isolation of human rotavirus in cell cultures and its antigenic and physicochemical properties (author's transl)].		1981	6278754
[variation of diarrhea viruses detected in japan with special reference to spherical viruses other than rotaviruses (author's transl)].		1981	6278755
[rotavirus gastroenteritis. laboratory diagnosis and study methods].		1981	6278777
the 30- to 54-nm rotavirus-like particles in gastroenteritis: incidence and antigenic relationship to rotavirus.	the 30- to 54-nm rotavirus-like particles were observed in the stool specimens of 17 children with gastroenteritis. these small rotavirus-like particles were shown to be antigenically related to the commonly described 68-nm rotavirus using the techniques of immune electron microscopy and elisa (enzyme-linked immunosorbent assay). four specimens containing the small rotavirus-like particles were shown to contain an antigen of a common human rotavirus serotype (type 2). the findings of small rotav ...	1981	6278067
treatment of diarrhea caused by rotavirus.		1981	6261130
[corona-, rota- and parvovirus infection in calves from a clinical point of view (author's transl)].		1981	6279381
evaluation of a new enzyme-linked immunosorbent assay test for rotavirus antigen in faeces.	a new commercial test for the diagnosis of rotavirus gastroenteritis was assessed. with some modifications it compared favourably with electron microscopy and immunofluorescence.	1981	6276444
rotavirus infection in children hospitalized for diarrhoea in argentina.	an epidemiological survey was undertaken to establish the importance of rotavirus as a cause of admission of children to hospital in buenos aires, argentina. between august 1978 and january 1980 rotavirus infection was detected in 62 (34%) of 180 infants and children admitted to hospital with acute gastro-enteritis. in identification of the virus, electron microscopy (em) was found to be as sensitive as counterimmunoelectrophoresis (cie). there was agreement between em and cie diagnosis in 90.5% ...	1981	6185065
new enteric viruses in the dog.		1981	6275684
viruses and bacteria in pediatric diarrhea in thailand: a study of multiple antibiotic-resistant enteric pathogens.	children with diarrhea admitted to a rehydration ward of a children's hospital in bangkok were investigated to determine the prevalence of enteric pathogens, the extent of children's previous antibiotic therapy, and the frequency of plasmid-mediated antibiotic resistance among infecting bacteria. rotavirus (36%), enterotoxigenic escherichia coli (18%), shigella (9%), salmonella (6%), campylobacter jejuni/coli (4%), and vibrio cholerae (2%) infections were among 105 children with diarrhea. antibi ...	1981	6275727
lymphocytotoxic and microbial antibodies in crohn's disease and matched controls.	patients with crohn's disease and age, sex and seasonally matched healthy controls were studied for cold lymphocytotoxic and a variety of microbial antibodies. lymphocytotoxic antibody titers were increased significantly in the crohn's patients compared to controls, but did not correlate with any of the microbial titers. antibodies to a pseudomonas-like bacterium and bacteroides vulgatus were also increased in the patients, but titers to chlamydia trachomatis, rotavirus and norwalk virus were no ...	1981	6275790
morphogenesis of human rotavirus type 2 wa strain in ma 104 cells.	morphogenesis of human rotavirus type 2 wa strain in ma 104 cells was observed. the virus antigen in the cytoplasm was detected by indirect immunofluorescence twelve hours after infection. the cytopathic effect occurred 24 hours after infection when virus particles were detected by em in the culture fluid as well as in thin sections of the infected cells. virus particles were observed in the dilated rer, nuclear envelope (perinuclear space), viroplasm, and a lysosome-like body. three types of vi ...	1981	6277282
viral diarrhea of young animals: a review.		1981	6277556
[evolution of anti-rota virus antibodies in the milk of cows treated in the last month of pregnancy either by adjuvated rotavirus vaccine or by the adjuvant fraction of the vaccine (author's transl)].		1981	6277557
a sensitive method for the production of diagnostic fingerprints of the genome segments of field isolates of rotavirus.	the 3'-terminal labelling procedure for analyzing the genome segment profile of field isolates of rotavirus (clarke and mccrae, 1981, j. virol. methods 2, 203) has been further developed to produce a rapid and sensitive method for generating diagnostic fingerprints from individual species of double-stranded rna. the fingerprints were obtained by a one-dimensional resolution of overlapping terminally labelled oligonucleotides produced by partial nuclease digestion with a base-specific nuclease. t ...	1981	6277973
detection of rotavirus immune complexes: relationship between rotavirus antibodies and rotavirus antigens in faeces.	an elisa was developed for the identification of rotavirus immune complexes in pig faeces. the relationship between rotavirus antigens, rotavirus immune complexes and rotavirus antibodies of iga class was examined.	1981	6277974
[rotavirus in children with acute gastroenteritis].		1981	6267410
rotavirus detection for the routine laboratory.		1981	6267531
distribution of antibodies to rotavirus in serum and lacteal secretions of naturally infected swine and their suckling pigs.	rotavirus antibodies were demonstrated in lacteal secretions and sera of 20 parturient sows and in sera of their newborn by an enzyme-linked immunosorbent assay blocking technique, using bovine rotavirus cell culture antigen and monospecific antibody to bovine rotavirus. antibodies to rotavirus occur in the 3 immunoglobulin (ig) classes igm, iga, and igg in lacteal secretions. high and long-persisting antibody activity was mainly associated with the iga class. the igm and igg decreased to undete ...	1981	6267967
comparative virulence of different bovine rotavirus isolates.	intestinal loops, ligated in colostrum-deprived calves were used to compare the virulence of four isolates of bovine rotavirus. histopathological studies were carried out on infected and control loops and measurements of villous length, crypt depth, villus:crypt ratio and crypt mitotic index were recorded. pathological changes associated with the rotaviruses included villous atrophy, flattening of absorptive epithelium and reduced villus:crypt ratios. the changes were confined to infected intest ...	1981	6268264
diarrhea in lambs: experimental infections with enterotoxigenic escherichia coli, rotavirus, and cryptosporidium sp.	thirteen gnotobiotic lambs, aged from a few hours to 8 days, were inoculated orally with single infections of enterotoxigenic escherichia coli (etec) (four animals), lamb rotavirus (five animals), and cryptosporidium (four animals). six gnotobiotic and two specific-pathogen-free lambs were co-inoculated with either rotavirus and etec (four animals), rotavirus and cryptosporidium (two animals), or etec and cryptosporidium (two animals). lambs 4 days of age and older became only subclinically infe ...	1981	6268543
effect of fractions of ethiopian and norwegian colostrum on rotavirus and escherichia coli heat-labile enterotoxin.	samples of colostrum from both ethiopian and norwegian women contained antirotavirus activities of immunoglobulin and non-immunoglobulin nature. no significant differences in rotavirus immunoglobulin a or in rotavirus-inhibiting activity were found between samples from the two countries. the non-immunoglobulin inhibitory activity was trypsin sensitive and heat stable (100 degrees c for 10 min). escherichia coli heat-labile enterotoxin antibodies were measured in the colostrum samples by enzyme-l ...	1981	6268544
susceptibility of mice to rotavirus infection: effects of age and administration of corticosteroids.	we examined susceptibility to the murine rotavirus, epizootic diarrhea of infant mice virus (edim), in normal suckling and weaned mice and in suckling mice treated with glucocorticoids. normal mice 1 to 40 days old were inoculated by gastrin intubation with high doses of edim and subsequently evaluated for rotavirus infection by solid-phase radioimmunoassay, by electron microscopy of intestinal tissue sections or by both. radioimmunoassay and electron microscopy showed a concordance of 89.5% in ...	1981	6268547
immuno-electron microscopic study on the antigenicity of tubular structures associated with human rotavirus.	tubular structures associated with human rotavirus were antigenically related to the single-capsid layered particles having only an inner capsid of the virus because they were agglutinated by heterologous bovine rotavirus antiserum.	1981	6268548
the use of markers in immune electron microscopy.	immune electron microscopy (iem) cannot be used successfully for structures that do not have recognisable morphology. however, at least some of these structures or components are related antigenically to recognisable antigens or viruses. we have therefore mixed unknown antigens with known markers and looked for the presence of mixed aggregates. the present study examined a low molecular weight subunit of rotavirus and a micellar form of hepatitis b surface antigen. in both cases mixed immune agg ...	1981	6268650
comparison of immunosorbent electron microscopy, enzyme immunoassay and counterimmunoelectrophoresis for detection of human rotavirus in stools.	the detection of human rotaviruses by routine electron microscopy examination of stool specimens has been compared with the sensitivity of detection obtainable by three different immunoassays. these assays are: 1) immunosorbent electron microscopy (isem), which consists of the serological trapping of viruses on electron microscopy grids coated with protein a and specific viral antiserum; 2) an enzyme-linked immunosorbent assay (elisa), in which the primary antibody is rabbit anti-rotavirus immun ...	1981	6268652
acute diarrhea and rotavirus infection in newborn babies and children in yogyakarta, indonesia, from june 1978 to june 1979.	a longitudinal study of acute diarrhea in children in yogyakarta, indonesia (june 1978 to june 1979), showed little variation throughout most months of the year in numbers of children admitted to hospital and in numbers infected with rotaviruses. both decreased during november and december coincidentally with seasonal change from dry to wet conditions. rotavirus particles were identified by electron microscopy in fecal specimens from 126 of 334 (38%) infants and children with acute diarrhea. nos ...	1981	6268656
improved detection of viruses by electron microscopy after direct ultracentrifuge preparation of specimens.	we have adapted the beckman airfuge air turbine ultracentrifuge and the new em-90 particle-counting rotor to improve detection by electron microscopy of viruses in clinical specimens. samples were clarified by centrifugation, pelleted in the em-90 rotor directly to formvar-coated copper grids, and strained with 1.5% sodium phosphotungstate. virus counts and endpoint titrations of serial dilutions of partially purified preparations of poliovirus, sa11 rotavirus, herpes simplex virus, and vaccinia ...	1981	6268658
twenty-five years of progress in understanding major infectious diseases of dairy cattle.		1981	6268673
a prospective study of rotavirus infection in infants and young children.	diarrhea in neonates, followed as a cohort, and their families was studied prospectively. the families were followed for an average of 16.3 months. stool and serum specimens were obtained at least every three months. stool specimens were examined for viruses by electron microscopy and cultured for enteropathogens, and serum specimens were tested for antibodies to rotavirus and norwalk virus. during the study, 237 episodes of gastroenteritis were observed in 104 infants and their 62 siblings. rot ...	1981	6268713
rotavirus infection in thai people: a preliminary study.		1981	6268729
molecular biology of rotaviruses. i. characterization of basic growth parameters and pattern of macromolecular synthesis.	the united kingdom tissue-adapted bovine rotavirus growing in african green monkey kidney (bsc-1) cells was selected as a model system with which to study the detailed molecular virology of rotavirus replication. study of the kinetics of infectious virus production revealed a fairly rapid replication cycle, with maximum yield of virus after 10 to 12 h at 37 degrees c. progeny genome synthesis was first detected during the virus latent period at 2 to 3 h postinfection. study of the kinetics of vi ...	1981	6268838
[ultrastructural characteristics of the rotavirus detected in swine with gastroenteritis].		1981	6268949
[clinical aspects of epidemic rotavirus gastroenteritis in adults].		1981	6269232
bovine rotavirus-cell interactions: effect of virus infection on cellular integrity and macromolecular synthesis.		1981	6269294
outbreak of rotavirus gastroenteritis among premature infants.		1981	6269687
distinctive ribonucleic acid patterns of human rotavirus subgroups 1 and 2.	the ribonucleic acid migration patterns of 7 subgroup 1 and 16 subgroup 2 human rotaviruses recovered from four geographic areas were compared. the subgroup 1 ribonucleic acid patterns had strikingly slower-moving segments 10 and 11, suggesting a correlation between the ribonucleic acid pattern and the subgroup specificity.	1981	6270002
a survey of virus particles in infantile gastroenteritis using the electron microscope.		1981	6270037
difference in antibody reactivity between complement fixation and immune adherence hemagglutination tests with virus antigens.	complement-fixing (cf) immunoglobulin m antibody to infantile gastroenteritis virus (a rotavirus) did not show the reactivity of immune adherence hemagglutination (iaha). early immunoglobulin g cf antibody produced both in patients and in guinea pigs experimentally infected with japanese encephalitis virus (a flavivirus) had weak reactivity in iaha test. however, late antibody showed higher titers by iaha than by cf. these results suggested that early antibodies with lower affinity are inefficie ...	1981	6270189
rapid, simple method of preparing rotaviral double-stranded ribonucleic acid for analysis by polyacrylamide gel electrophoresis.	a procedure for extracting rotaviral double-stranded ribonucleic acid (rna) directly from fecal and intestinal specimens collected from calves and pigs is described. this procedure provides a rapid, simple, reproducible method of obtaining rotaviral double-stranded rna preparations suitable for electrophoretic analysis in polyacrylamide-agarose composite gels. the rotaviral genome electrophoretic migration pattern produced by double-stranded rna extracted directly from a specimen by this procedu ...	1981	6270190
evaluation of enzyme immunoassays for the detection of human rotavirus.		1981	6270217
isolation and characterization of feline rotavirus.	feline rotavirus was detected by electron microscopy in the faecal samples of a cat, and was propagated in an established cell line of foetal rhesus monkey kidney, ma104, cell cultures. morphologically, feline rotavirus was indistinguishable from known rotaviruses. complete particles showed a characteristic "spoke-like' arrangement of inner capsomers surrounded by an outer layer. intracytoplasmic inclusion bodies in different sizes and shapes were produced in infected ma104 cells. reproducible c ...	1981	6270251
isolation from chickens of a rotavirus lacking the rotavirus group antigen.	a virus, designated 132 virus, was isolated from the faeces of chickens in chick embryo liver cell cultures. the morphology and morphogenesis of 132 virus were indistinguishable from that of rotaviruses. the nucleic acid of 132 virus had the nuclease resistance of double-stranded rna, and was separated by polyacrylamide gel electrophoresis into 11 segments with mol. wt. ranging from 2.07 x 10(6) to 0.20 x 10(6). spf chickens were susceptible to oral infection with 132 virus, which replicated in ...	1981	6270272
proteolytic enhancement of rotavirus infectivity: molecular mechanisms.	the polypeptide compositions of single-shelled and double-shelled simian rotavirus particles were modified by exposure to proteolytic enzymes. specifically, a major outer capsid polypeptide (vp3) having a molecular weight of 88,000 in double-shelled particles was cleaved by trypsin to yield two polypeptides, vp5* and vp8* (molecular weights, 60,000 and 28,000, respectively). the cleavage of vp3 by enzymes that enhanced infectivity (trypsin, elastase, and pancreatin) yielded different products co ...	1981	6270356
in vitro transcription catalyzed by heat-treated human rotavirus.	the in vitro characteristics of human rotavirus transcription have been examined. the virus has an associated rna polymerase activity which was activated after a heat shock treatment. the enzyme required the presence of the four ribonucleoside triphosphates and a divalent cation (mg2+), and it required an optimum ph of 8.5. the polymerase was activated by monovalent salts and inhibited by na ppi. the addition of actinomycin d, alpha-amanitin, or rifampin did not inhibit the polymerase activity. ...	1981	6270365
rota-coronavirus vaccination of pregnant cows.		1981	6270525
[current theories on the etiology of crohn disease].		1981	6270624
[morphogenesis of human rotavirus in a cell culture].	morphogenesis of human rotavirus was studied by primary green monkey kidney cell culture by electron microscopy, at the levels of the 5th and 12th passage in the cell culture. the initial stages of rotavirus morphogenesis were shown to be associated with cytoplasmic inclusions. nucleoid or core particles formed in the periphery of the inclusions were transported by budding to endoplasmic reticulum cavities where the final stages of virion maturation occurred. the loss of the outer membrane seems ...	1981	6270913
isolation of cytopathic porcine rotavirus in cell roller culture in the presence of trypsin.	cytopathic porcine rotavirus was isolated in roller tube cultures of ma-104 cells. faeces of a piglet suffering from diarrhoea, were treated with trypsin which was also added to the maintenance medium. using stationary cultures, virus was not isolated from the same materials. the cytopathic effect was clearly observed after 8 serial passages and the virus titer at the 14th passage level was 10(7) median tissue culture infective doses per ml, or higher. at the 27th passage, rotavirus particles we ...	1981	6271098
[persistence of transplacental antibodies against rotavirus in children less than 6 months of age].	the persistence of antibodies against rotavirus was studied in the sera of 54 recently born infants up to six months of age; likewise, in the sera of their mothers. serum positivities were found to be similar in both, showing 96% for those with antibodies and 94.4% for the latter. the percentage of infants with antibodies dropped gradually to the 4th month of age and since then rises in the titre of antibodies began to appear in four infants, which indicated there had been rotavirus infections. ...	1981	6271147
aetiology of viral gastroenteritis: a review.	over the past seven to eight years, several virus groups have been shown to be associated with gastroenteritis. they are adenoviruses, astroviruses, caliciviruses, coronaviruses. norwalk-like viruses and rotaviruses. in infants and young children, rotaviruses are the single most important aetiological agents of acute gastroenteritis in terms of numbers of cases and also of patients requiring admission to hospital. in older children and adults, the aetiology is less clear, but the present state o ...	1981	6272071
campylobacter as a cause of acute enteritis in children in south australia. i. a 12-month study with controls.	during a 12-month period, we tested faecal samples from 386 children with acute enteritis and 332 controls by light and electron microscopy, and by bacterial and viral culture for pathogens, especially to assess the importance of campylobacter. campylobacter alone was responsible for the illness in 17 patients (5%), and was second to salmonella among the bacterial agents, which were predominant in summer. overall, rotavirus was the commonest identifiable cause of acute enteritis and was especial ...	1981	6272073
campylobacter as a cause of acute enteritis in children in south australia. ii. clinical comparison with salmonella, rotavirus and non-specific enteritis.	the clinical features of 17 children with campylobacter enteritis were compared with 17 age- and sex-matched children with enteritis due to salmonella, rotavirus or those in whom there was no identifiable pathogen. prominent clinical features of campylobacter enteritis included fever, diarrhoea, vomiting, blood in stools and periumbilical pain. dehydration was uncommon, compared to rotavirus and non-specific enteritis. the acute illness was self-limited, in spite of prolonged asymptomatic faeca ...	1981	6272074
[studies on an improved technic to increase the positive detection rate of rotavirus under electron microscope (author's transl)].		1981	6273004
travelers' diarrhea in panamanian tourists in mexico.	to determine whether residents of developing countries are unlikely to acquire travelers' diarrhea, 64 panamanians of widely divergent socioeconomic strata were studied during a 15-day tour through mexico. twenty-three (36%) tourists experienced 27 episodes of travelers' diarrhea that were caused by seven different pathogens. the most commonly identified etiologic agents were rotavirus (26%), norwalk virus (15%), and campylobacter fetus (11%), whereas enterotoxigenic escherichia coli was not fre ...	1981	6273474
nonenteric sources of rotavirus in acute diarrhea.		1981	6273477
composition and topography of structural polypeptides of bovine rotavirus.	the composition and topography of the structural polypeptides of bovine rotavirus was studied by polyacrylamide gel electrophoresis of radioactively labelled virus grown in llc-mk2 cells and by lactoperoxidase-catalysed iodination of single- and double- capsid particles. bovine rotavirus was found to possess at least six structural polypeptides, three of them associated with the inner capsid (p102k, p91k and p45k) and the others with the outer capsid (p84k, p37k and p34k). the most abundant poly ...	1981	6273501
lactose malabsorption following rotavirus infection in young children.		1981	6273520
comparison of three electron microscopy techniques for the detection of human rotaviruses.	rotavirus detection by direct electron microscopy was compared with direct and indirect immune electron microscopy techniques. the latter two approaches permitted the enumeration of 25 and 103 times more rotaviruses respectively, than direct electron microscopy. also, 70% and 90% of the virus particles were aggregated by direct and indirect immune electron microscopy techniques respectively, thus facilitating their detection.	1981	6273695
sequential passages of human rotavirus in ma-104 cells.	starting with a small amount of diarrheal feces containing human rotavirus (hrv), we succeeded in propagation of the virus using the roller culture technique with ma-104 cells. furthermore, we made a successful adaptation of hrv to a stationary culture and developed a plaque assay for the cell culture-adapted viruses. the 3 culture-adapted virus isolates, ku, yo, and 44 produced plaques (about 0.5-1.0 mm in diameter) under the overlay medium consisting of 0.6% purified agar, 3 micrograms of acet ...	1981	6273696
neutralizing patterns of anti-bovine rotavirus (lincoln) serum against cytopathic bovine rotaviruses isolated from calves in japan.		1981	6273700