Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| alkaline deoxyribonuclease induced by herpes simplex virus type 1: composition and properties of the purified enzyme. | 1980 | 6247825 | |
| in vitro and in vivo resistance of herpes simplex virus to 9-(2-hydroxyethoxymethyl)guanine (acycloguanosine). | in vitro passage of the patton strain of herpes simplex virus type 1 (hsv-1-p) in the presence of acycloguanosine (acg) led to the emergence of a highly drug-resistant strain (hsv-1-p-acg-r). over 1% of virions in 6 of 15 hsv strains tested were able to form plaques in the presence of 10 mumol of acg on first exposure to the drug. therefore, there exists among hsv strains a broad range of susceptibilities to acg, and some strains contain particles which are partially resistant to acg before ever ... | 1980 | 6247967 |
| integration site(s) of herpes simplex virus type 1 thymidine kinase gene and regional assignment of the gene for aminoacylase-1 in human chromosomes. | to investigate the chromosomal sites of integration of the herpes simplex virus type 1 (hsv-1) thymidine kinase (tk) gene in hsv-1-transformed human hela(bu25)/kos 8-1 cells, the biochemically transformed cells were fused with tk-negative mouse lm(tk-) cells, and human-mouse somatic cell hybrid lines (lh81) were isolated using a hatg-ouabain selection system. the presence of hsv-1 tk activity in the hybrid lines was verified by disc polyacrylamide gel electrophoresis (page) and by enzyme neutral ... | 1980 | 6248297 |
| oxime and dithiolane derivatives of 5-formyl-2'-deoxyuridine and their 5'-phosphates: antiviral effects and thymidylate synthetase inhibition. | 5-formyl-2'-deoxyuridine (2a), an effective inhibitor of herpes simplex virus type 1 or 2 (hsv-1, hsv-2) and vaccinia virus, was converted to the oxime (3a) and dithiolane (4a) derivatives. the oxime (3a) was equally as potent as the formyl compound against hsv-1, but one-fifth as active against hsv-2, 100 times less effective against vaccinia, and 25 times less toxic for the host cells. in addition, compound 3a was about 10 times less active than 2a in inhibiting thymidylate synthetase in vivo ... | 1980 | 6248643 |
| combination chemotherapy: interaction of 5-methoxymethyldeoxyuridine with adenine arabinoside, 5-ethyldeoxyuridine, 5-iododeoxyuridine, and phosphonoacetic acid against herpes simplex virus types 1 and 2. | the antiviral activity of 5-methoxymethyl-2'-deoxyuridine (mmudr) was compared with that of 5-iodo-2'-deoxyuridine (iudr), 5-ethyl-2'-deoxyuridine (etudr), adenine arabinoside (ara-a), and phosphonoacetic acid (paa) against herpes simplex virus type 1 (hsv-1) and type 2 (hsv-2). mmudr was more potent than ara-a and paa but less active than etudr and iudr against hsv-1 in rabbit kidney (rk-13) cells. in vero cells, the antiviral activities of mmudr, ara-a, and paa against hsv-1 were of the same o ... | 1980 | 6249191 |
| 2'-fluoro-5-iodo-aracytosine, a potent and selective anti-herpesvirus agent. | a newly synthesized pyrimidine analog, 2'-fluoro-5-iodo-aracytosine (fiac), suppressed by 90% the replication of various strains of herpes simplex virus types 1 and 2 at concentrations of 0.0025 to 0.0126 microm. cytotoxicity was minimal, as determined by trypan blue dye exclusion with norman vero, wi-38, and nc-37 cell proliferation; the 50% inhibitory dose was 4 to 10 microm in a 4-day assay. when compared with other antiviral drugs, fiac was active at much lower concentrations than arabinosyl ... | 1980 | 6249196 |
| marrow-dependent cells depleted by 89sr mediate genetic resistance to herpes simplex virus type 1 infection in mice. | adult mice resistant to infection with 10(6) plaque-forming units of a virulent strain of herpes simplex virus type 1 were treated with 89sr to abrogate marrow-dependent cell functions. treated mice were found to be much more susceptible to the herpes simplex virus type 1 infection than untreated mice. the virus persisted in the visceral tissues of 89sr-treated mice for 3 or more days postinfection but not in those of untreated mice. the virus also spread to the spinal cords of treated but not u ... | 1980 | 6249746 |
| differences in attachment between herpes simplex type 1 and type 2 viruses to neurons and glial cells. | fractions of nerve cell perikarya, synaptosomes, and astrocytic glia were prepared from human, monkey , rabbit, rat, and mouse brain tissue. the herpes simplex virus type 1 (hsv-1) and type 2 (hsv-2) binding capacity of these fractions was studied. pretreatment of fractions with one type of hsv and the subsequent testing of adsorption of homotypic and heterotypic virus ws employed to reveal type selectivity of virus binding receptors. a higher density of hsv-1 than of hsv-2 selective receptors w ... | 1980 | 6249749 |
| ag-4 complement-fixing antibodies in cervical cancer and herpes-infected patients using local herpes simplex virus type 2. | the incidence of anti-ag-4 complement-fixing antibodies in australian cervical carcinoma (cacx), herpes simplex virus (hsv) type 1 and 2, and control patients, was investigated using local hsv strains. the local hsv strains (both hsv-imi and hsv-2mi) were found, by neutralization experiments, to vary from the american prototype strains. all hsv-2-strains tested were able to induce ag-4 in 4-h infected hep-2 cells. anti-ag-4-complement-fixing antibodies were detected in 40% of dysplasia patients, ... | 1980 | 6249764 |
| cell-mediated immunity in herpes simplex virus-infected mice: suppression of delayed hypersensitivity by an antigen-specific b lymphocyte. | lymph node cells, regional to the site of infection with herpes simplex virus type 1 (hsv-1), when taken 1 to 9 months p.i. and transferred to hsv-1 immune recipients, suppressed the delayed type hypersensitivity (dth) reaction to the virus. the suppressive activity was specific for hsv-1 and was transferred by a thy-1.2-negative, ig-positive, lymphocyte population. anti-hsv-1 serum did not suppress the hsv-1-induced dth response. contralateral lymph nodes contained little or no suppressor cell ... | 1980 | 6249886 |
| influence of 2,4,6-triamino-pyrimidyl-5-azobenzene on the activities of acid phosphatase, alkaline phosphatase and lactate dehydrogenase in cell cultures infected with herpes simplex virus type 1. | treatment of vero cells with 2,4,6-triamino-pyridimyl-5-azobenzene (10 gamma/ml) one hour after inoculation of herpes simplex virus type 1 limits the influence of the virus infection, consisting in the enchancement of the activities of alkaline phosphatase, acid phosphatase and ldh (especially of the latter two enzymes) and in changes in the isoenzyme patterns of acid and alkaline phosphatases and of peroxidase. in general the influence of the drug is expressed both by the fact that enzyme level ... | 1980 | 6250280 |
| antiviral activity of tunicamycin on herpes simplex virus. | tunicamycin (0.5 microgram/ml) significantly lowers (2 to 3 log10) the infectious yield of herpes simplex virus type 1 grown in chicken embryo fibroblasts and in bsc1 cells. although virus particles are formed and the synthesis of the viral deoxyribonucleic acid is only partially affected by the antibiotic, the glycosylation of herpesvirus glycopeptides is amost completely inhibited. the morphology of virus particles made in the presence of tunicamycin is similar to that of intact virus particle ... | 1980 | 6250466 |
| inhibition of herpes simplex virus multiplication by the pokeweed antiviral protein. | the pokeweed antiviral protein inhibited the multiplication of herpes simplex virus type 1 in cell culture. the extent of antiviral activity was proportional to the length of time that the antiviral protein was present postinfection. the results demonstrate that the continued presence of the pokeweed antiviral protein is necessary for the maximum inhibition of virus yields. | 1980 | 6250468 |
| restriction of herpes simplex virus by ama 1 cells. an analysis of viral macromolecule synthesis. | ama 1 cells, and alpha-amanitin-resistant subline of cho cells, restricted herpes simplex virus-1 and -2 replication. the infection was characterized by i) induction of typical cytopathology; ii) appearance of all the major virus proteins, glycoproteins and dna earlier than in hep-2 cells, followed by shut off of virus macromolecule synthesis; iii) defective maturation of viral particles, i.e. scarce assembly and lack of envelopment. the early shut off of viral dna and protein synthesis, and the ... | 1980 | 6250513 |
| electron microscopic studies of herpes simplex virus type 1 infection of macrophages, t- and b-lymphocytes of mice. | the course of infection with herpes simplex virus type 1 (hsv-1) in peritoneal macrophages, phytohemagglutinin (pha-)stimulated (t-)lymphocytes and lipopolysaccharide (lps-)stimulated (b-)lymphocytes of nmri-mice was studied by means of electron microscopy. non-stimulated as well as thioglycolate-stimulated macrophages were investigated; lymphocytes were derived both from hsv-1-sensitized and non-sensitized animals. the morphological characteristics of the abortive infection in macrophages and t ... | 1980 | 6250515 |
| properties of herpes simplex virus type 1 and type 2 dna polymerase. | herpes simplex virus type 1 (hsv-1) and type 2 (hsv-2) dna polymerases were highly purified from infected hela bu cells by deae cellulose, phosphocellulose and dna cellulose column chromatography. dna exonuclease activity but not endonuclease activity was found associated with both types of dna polymerase. both dna polymerase activities could be activated by salt in a similar fashion with the optimal activity in the range of ionic strength between 0.22 and 0.29 alpha. at an ionic strength of 0.1 ... | 1980 | 6250618 |
| a serological study of herpes simplex virus type 1 antibody over a 13-year period. | hsv-1 serum antibody titers determined by 50% plaque reduction and confirmed by radioimmunoassay in 11 samples from an individual over a 13-year period indicated a significant increase between the first sample and a sample taken 9 years later. this increase did not seem to reflect loss of antibody in the low titered serum sample due to storage. | 1980 | 6250922 |
| isolation and translation of mrna encoded by a specific region of the herpes simplex virus type 1 genome. | we have examined in detail the major mrna species encoded by the region of the herpes simplex virus type 1 genome encoded by hindiii fragment k (0.53-0.59 from the left end of the prototype arrangement of the genome) by using this restriction fragment bound to cellulose as a reagent for isolation of this mrna. before viral dna replication in infected cells (early), a major species of viral mrna 5.2 kilobases (kb) in length is abundant. after the onset of viral dna replication (late), four mrna s ... | 1980 | 6251246 |
| genetic studies of cell fusion induced by herpes simplex virus type 1. | eight cell fusion-causing syn mutants were isolated from the kos strain of herpes simplex virus type 1. unlike the wild-type virus, the mutants produced plaques containing multinucleated cells, or syncytia. fusion kinetics curves were established with a coulter counter assay for the mutants and wild-type virus in single infections of human embryonic lung (hel) cells, for the mutants and wild-type virus in mixed infections (dominance test), and for pairs of mutants in mixed infections (complement ... | 1980 | 6251259 |
| alterations in glycoprotein gb specified by mutants and their partial revertants in herpes simplex virus type 1 and relationship to other mutant phenotypes. | the tsb5 mutant of herpes simplex virus type 1 (hsv-1) strain hfem was shown previously to be temperature sensitive for accumulation of the mature form of glycoprotein gb, for production or activity of a factor required in virus-induced cell fusion, and for production of virions with normal levels of infectivity. in addition, a previous study showed that virions produced by tsb5 at permissive temperature were more thermolabile than hfem virions and contained altered gb that did not assume the di ... | 1980 | 6251260 |
| persistence of herpes simplex virus genes in cells of neuronal origin. | the growth characteristics of the kos strain of herpes simplex virus type 1 (hsv-1) in cell lines of nervous tissues origin were examined in an attempt to develop a tissue culture system mimicking the in vivo state of hsv-1 latency. we have previously reported that the b103 rat brain neuroma cell line is nonpermissive for growth of the kos strain. in this report, we show that this nonpermissiveness is a temperature- and multiplicity-dependent phenomenon, with minimum virus yields at an elevated ... | 1980 | 6251268 |
| infections of susceptible and resistant mouse strains with herpes simplex virus type 1 and 2. | the spread of hsv of type 1 and 2 was investigated after intraperitoneal, intraplantar and intracerebral infections of resistant (c57/bl) and susceptible (nmri) mice. the virus spreads after i.p. infection to the spleen and the liver to the same extent in both strains of mice. however, virus is eliminated earlier in resistant mice. intracerebral infections revealed a peculiar type of resistance of c57/bl mice especially for type 2 of hsv. hsv multiplies in the thymus at the early stage of infect ... | 1980 | 6251783 |
| experimental infection and the state of viral latency of adult tupaia with herpes simplex virus type 1 and 2 and infection of juvenile tupaia with temperature-sensitive mutants of hsv type 2. | the susceptibility of adult tupaia belangeri to infection with herpes simplex virus (hvs) was investigated. adult animals were inoculated intraperitoneally with hsv type 1 or 2. with the exception of hsv-2, strain hg-52, 10(5)--10(6) pfu of all hsv strains caused lethal infection irrespective of the age of the animals. infections hsv was recovered from the spinal cord of those animals which had survived infection with a low dose of virus. the dna of the recovered viruses was compared to the dna ... | 1980 | 6251787 |
| latency competence of thirteen hsv-1 temperature-sensitive mutants. | thirteen temperature-sensitive (ts) mutants of hsv-1 were analysed for their capacity to establish latent infections in the brains of mice. eleven of the mutants could be classified as latency-positive or -negative; two could not be assigned to either group. leakiness of mutants in the brain and differences in particle/infectivity ratios were found not to play a role in the results. ts+ revertants of selected latency-negative mutants regained the capacity to establish latent infections, indicati ... | 1980 | 6252286 |
| ultraviolet irradiation of herpes simplex virus (type 1): delayed transcription and comparative sensitivites of virus functions. | the delay in the replication of herpes simplex virus surviving u.v. irradiation occurs after the uncoating of virus, as judged by sensitivity to dnase. it occurs before translation, judged by the kinetics of appearance of various virus-specific proteins, and before transcription, judged by the detection of virus-specific rna by in situ hybridization. since the delays in both transcription and translation are reversed by photoreactivation, the simplest hypothesis is that pyrimidine dimers directl ... | 1980 | 6252290 |
| mutant analysis of herpes simplex virus-induced cell surface antigens: resistance to complement-mediated immune cytolysis. | bhk-21 cells infected with temperature-sensitive mutants of herpes simplex virus type 1 strain kos representing 16 complementation groups were tested for susceptibility to complement-mediated immune cytolysis at permissive (34 degrees c) and nonpermissive (39 degrees c) temperatures. only cells infected by mutants in complementation group e were resistant to immune cytolysis in a temperature-sensitive manner compared with wild-type infections. the expression of group e mutant cell surface antige ... | 1980 | 6252331 |
| herpes simplex virus phosphoproteins. ii. characterization of the virion protein kinase and of the polypeptides phosphorylated in the virion. | the protein kinase associated with purified herpes simplex virus 1 and 2 virions partitioned with the capsid-tegument structures and was not solubilized by non-ionic detergents and low, non-inhibitory concentrations of urea. the enzyme required mg2+ or mn2+ and utilized atp or gtp. the activity was enhanced by non-ionic detergents and by na+ even in the presence of high concentrations of of mg2+, but not by cyclic nucleotides. the enzyme associated with capsid-tegument structures phosphorylated ... | 1980 | 6252339 |
| preparation and characterization of specific antisera to individual glycoprotein antigens comprising the major glycoprotein region of herpes simplex virus type 1. | the major glycoprotein complex (vp123) of herpes simplex virus type 1 resolved by sodium dodecyl sulfate (sds)-polyacrylamide gel electrophoresis was purified and further fractionated into two major and two minor components by chromatography of the isolated vp123 region on sds-hydroxylapatite columns. the two major components (gc and ga/gb) were purified free of other polypeptides and used to prepare specific antisera to these glycoproteins. radioimmune precipitation demonstrated that these anti ... | 1980 | 6252346 |
| 5'-amino-5'-deoxythymidine: synthesis, specific phosphorylation by herpesvirus thymidine kinase, and stability to ph of the enzymically formed diphosphate derivative. | radioactive 5'-amino-5'-deoxythymidine (5'-adthd), an inhibitor of herpes simplex virus, was synthesized by direct amination of monotosylated thymidine with concentrated ammonium hydroxide. incubation of 5'-adthd with purified herpes simplex virus type 1-encoded pyrimidine deoxyribonucleoside kinase produced the diphosphate derivative. the monphosphate derivative of 5'-adthd was not detected as a reaction product of this enzymatic phosphorylation. a purified mixture of nonviral thymidine kinase ... | 1980 | 6252834 |
| herpes simplex virus thymidine kinase transcripts are absent from both nucleus and cytoplasm during infection in the presence of cycloheximide. | nick-translated dna from a recombinant plasmid containing the herpes simplex virus type 1 thymidine kinase gene was used as a probe for the synthesis of thymidine kinase rna. the recombinant plasmid was generated by inserting the 3.5-kilobase fragment derived by bamhi digestion of herpes simplex virus type 1 dna into plasmid pbr322. at 8 h after infection, cytoplasmic and nuclear rna hybridized to 14% and 19% of the recombinant dna probe, respectively. however, no significant hybridization was f ... | 1980 | 6253662 |
| synthesis and processing of glycoproteins gd and gc of herpes simplex virus type 1. | herpes simplex virus type 1 (hsv-1) contains five glycoproteins, designated ga, gb, gc, gd, and ge. the present studies focused on the synthesis and processing of two of these, gc and gd. by using monoprecipitin antibody to gc, we demonstrated an antigenic and structural relationship between the precursor, pgc(110), and the product, gc(130). tryptic peptide analysis showed that pgc and gc shared methionine peptides and that these molecules had the same fingerprint pattern as that of gc(130) extr ... | 1980 | 6253668 |
| mechanism of inhibition of herpes simplex virus and vaccinia virus dna polymerases by aphidicolin, a highly specific inhibitor of dna replication in eucaryotes. | the inhibition in vitro of herpes simplex virus 1 and vaccinia virus dna polymerases by aphidicolin is primarily noncompetitive with dgtp, datp, dttp, dna, and mg2+ and competitive with dctp in analogy with the mode of inhibition of cellular alpha-polymerase. the degree of inhibition of viral or cellular growth in vivo can be quantitatively predicted by the degree of inhibition of the isolated replicative dna polymerases at the same concentration of aphidicolin in suitable conditions (limiting d ... | 1980 | 6253671 |
| herpes simplex virus dna sequences in the cns of latently infected mice. | it has been amply documented that herpes simplex virus (hsv) persists in sensory ganglia of the peripheral nervous system (pns). in contrast, hsv latency in the central nervous system (cns) has not been well characterized. corneal inoculation of virus results in a productive viral infection in the cns during the first week after inoculation, indicating that the virus can progress from the pns to the cns. during latency, hsv has been found by co-cultivation of cns tissue in only a very small frac ... | 1980 | 6253827 |
| cloning of reiterated and nonreiterated herpes simplex virus 1 sequences as bamhi fragments. | over 95% of the herpes simplex virus type 1 (strain f) dna sequences have been cloned as bamhi fragments in the pbr322 plasmid. with one exception, all of the cloned fragments have the same electrophoretic mobilities and restriction enzyme cleavage sites as do the authentic fragments derived from the bamhi digests of the viral genome. the exception is the bamhi b fragment mapping at the right end of l component in the prototype arrangement of the dna. thus, a small deletion mapping near the left ... | 1980 | 6254015 |
| dna-transformed murine teratocarcinoma cells: regulation of expression of simian virus 40 tumor antigen in stem versus differentiated cells. | thymidine kinase-deficient (tk-; atp:thymidine 5'-phosphotransferase, ec 2.7.1.21)f9 teratocarcinoma stem cells have been transformed with a recombinant plasmid genome consisting of the pbr322 genome linked to a herpes simplex virus type 1 thymidine kinase gene (hsv-1 tk) and a simian virus 40 (sv40) genome. a clonal line of stem cells was obtained that contains only one copy of plasmid dna, which is integrated into murine chromosomal dna through a site on the pbrr322 genome. the hsv-1 tk gene, ... | 1980 | 6254045 |
| latency of herpes simplex virus in absence of neutralizing antibody: model for reactivation. | mice inoculated with herpes simplex virus (type 1) by the lip or corneal route and then passively immunized with rabbit antibody to herpes simplex virus developed a latent infection in the trigeminal ganglia within 96 hours. neutralizing antibody to herpes simplex virus was cleared from the circulation and could not be detected in most of these mice after 2 months. examination of ganglia from the antibody-negative mice revealed latent virus in over 90 percent of the animals, indicating that seru ... | 1980 | 6254149 |
| comparison of indirect hemagglutination and 51chromium release tests for detection of herpes simplex virus type 1 and 2 antibodies in patients with recurrent herpes infections. | indirect hemagglutination and 51cr release tests (ihat and 51-crt respectively) were compared in patients with recurrent herpes simplex virus (hsv) infections from whom hsv-1 or hsv-2 was isolated. both tests were equally sensitive and specific in detecting hsv antibodies. however, ihat was more specific in detecting homologous hsv antibody response in patients with recurrent hsv-2 infections. past infections with hsv-1 in the patients with dual infections were detected by determining hsv-type s ... | 1980 | 6254476 |
| reaction patterns of herpes simplex virus type 1 and type 2 proteins with sera of patients with uterine cervical carcinoma and matched controls. | serum from 105 individuals with diagnosed uterine cervical cancer and 231 matched controls were examined for their ability to react with a large number of herpes simplex virus type 1 or type 2 (hsv-1, hsv-2) proteins. radiolabeled hsv-1 or hsv-2 proteins were mixed with test serum and immune complexes were isolated with staphylococcal protein a. viral proteins in the immune complexes were resolved by polyacrylamide gel electrophoresis and visualized by fluorography. when the frequency of precipi ... | 1980 | 6254649 |
| resistance to hsv-1 in the mouse is governed by two major, independently segregating, non-h-2 loci. | earlier studies showed that genetic resistance of adult, inbred strains of mice to herpes simplex virus-type 1 (hsv-1) is a dominant genetic trait. the present studies were undertaken to determine the number of genetic loci involved and whether they were found within the major histocompatibility complex, h-2, of the mouse. challenge with hsv-1 of progeny of mice backcrossed to moderately susceptible balb/c mice, of progeny of mice backcrossed to very susceptible a/j strain mice, and of progeny o ... | 1980 | 6254873 |
| the effect of polyamines on herpes simplex virus type 1 dna polymerase purified from infected baby hamster kidney cells (bhk-21/c13). | the dna polymerase whose synthesis is directed by the herpes simplex virus type 1 (hsv-1) dna was purified 545-fold from bhk-21/c13 cells 16 h after infection with the virus. spermidine and spermine stimulated the activity of the polymerase over the concentration range 0.5 mm to 2.5 mm. this effect was enhanced with increased concentrations of polyamine, maximum stimulation being threefold and fourfold for spermidine and spermine respectively. the diamine, putrescine, had little effect on the en ... | 1980 | 6255073 |
| the effect of delta-9-tetrahydrocannabinol on herpes simplex virus replication. | both herpes simplex virus type 1 (hsv-1) and herpes simplex virus type 2 (hsv-2) failed, in an identical fashion to replicate and produce extensive c.p.e. in human cell monolayer cultures which were exposed (8 h before infection, at infection, or 8 h p.i.) to various concentrations of delta-9-tetrahydrocannabinol. similar results were obtained with a plaque assay utilizing confluent monkey cells. possible mechanisms for this antiviral activity are discussed. | 1980 | 6255077 |
| quantification of plasminogen activator activity associated with herpesvirus-transformed cells. | herpesvirus-transformed cell lines were examined for plasminogen activator (pa) activity using a quantitative assay. previous results of cold fibrin overlay assays indicated that herpesvirus-transformed hamster cell lines produce fibrinolytic activity. quantification of this activity involved the use of an 125i-fibrin lysis assay in which medium previously incubated with transformed or normal cells was tested for its ability to lyse 125i-fibrin polymers. this assay indicated an enhanced producti ... | 1980 | 6255081 |
| nuclear processing of viral high-molecular-weight rna in cells infected with herpes simplex virus type 1. | nuclear rna isolated from cells infected by herpes simplex virus type 1, strain f, was fractionated on formamide-sucrose gradients into two major classes, greater and less than 45s. these two classes of labeled nuclear rna were hybridized to viral dna fragments generated by digestion with the restriction enzymes hindiii and bglii. early in infection, only a few dna fragments hybridized to rna, with slight differences between the two classes. late in infection, all dna fragments hybridized, showi ... | 1980 | 6255179 |
| comparative structural analysis of glycoprotein gd of herpes simplex virus types 1 and 2. | we studied the synthesis and processing of the type-common glycoprotein gd in herpes simplex virus type 2 (hsv-2) and compared it structurally to glycoprotein gd of herpes simplex virus type 1 (hsv-1). we demonstrated that in hsv-2, gd undergoes posttranslational processing from a lower-molecular-weight precursor (pgd51) to a higher-molecular-weight product (gd56). tryptic peptide analysis by cation-exchange chromatography indicated that this processing step altered neither the methionine nor th ... | 1980 | 6255183 |
| fine-structure mapping and functional analysis of temperature-sensitive mutants in the gene encoding the herpes simplex virus type 1 immediate early protein vp175. | herpes simplex virus (hsv)-specific proteins fall into at least three kinetic classes whose synthesis is sequentially and coordinaely regulated. temperature-sensitive (ts) mutants of one complementation group (1-2) are defective in the transition from immediate early to early and late protein synthesis. to elucidate the function of the 1-2 gene product in the hsv type 1 replicative cycle, nine ts mutants in this group were mapped by fine-structure analysis and characterized members of the group ... | 1980 | 6255206 |
| frequency and levels of antibodies to epstein-barr virus-specific dnase are elevated in patients with nasopharyngeal carcinoma. | sera from healthy individuals and patients with infectious mononucleosis, burkitt lymphoma, nasopharyngeal carcinoma, or other malignancies were examined for their capacity to neutralize epstein-barr virus (ebv)-induced dnase activity. sera were found that neutralized the ebv dnase but not herpes simplex virus type 1 or type 2 dnases, and vice versa. sera from 46 of the 49 patients with nasopharyngeal carcinoma examined (94%) neutralized > 6 units of ebv dnase per ml of serum. in contrast, only ... | 1980 | 6255477 |
| the possible relationship of herpes simplex virus infection to cause of retardation in severe mental handicap. | the relationship between herpes simplex virus type 1 and mental retardation is explored by studying the antibody levels to this virus in a group of 86 severely and profoundly retarded adults. a tendency towards higher antibody levels is found in patients whose retardation is of unknown aetiology. the relationship of these observations to previous research findings and the possible significance of herpes simplex virus in the aetiology of mental retardation are discussed. | 1980 | 6255507 |
| mapping of the herpes simplex virus dna sequences present in herpes simplex virus type-1 thymidine kinase-transformed cells. | analyses of the herpes simplex virus (hsv) dna sequences which are present in three hsv thymidine kinase-transformed (hsvtk+) mouse cell lines have revealed that these cells contain relatively large and variable portions of the viral genome. two of these cell lines do not contain the viral dna sequences known to encode the early viral genes normally responsible for regulating tk gene expression during lytic hsv infections. this finding suggests that cell-associated viral tk gene expression may b ... | 1980 | 6255619 |
| deoxyribonucleases in herpes simplex virus type 1 and 2 infected primary rabbit kidney cells. | in primary rabbit kidney cells infected with herpes simplex virus four different neutral deoxyribonuclease activities can be detected by means of the deoxyribonuclease assay in dna-containing polyacrylamide gels following their separation by disc-electrophoresis. the method is suitable to follow independently the change in each activity of the different enzymes using only about 5 x 10(5) cells for each assay during the time-course of infection. under these conditions one enzyme activity is const ... | 1980 | 6255696 |
| antiviral activity of antilipidemic compounds on herpes simplex virus type 1. | two antilipidemic compounds, clofibrate and procetofene, inhibited the replication of herpes simplex virus type 1 (hsv-1) in african green monkey kidney cells. clofibrate, at a concentration of 400 mumol/liter caused a 63% reduction (p < 0.001) in hsv-1 yield and at 100 mumol/liter caused a 62% reduction (p < 0.001) in plaque formation. two stereoisomeric analogs of clofibric acid, (-)- and (+)-desmethyl clofibric acid, also caused a significant inhibition of hsv-1 replication. procetofene at 5 ... | 1980 | 6255860 |
| erythro-9-(2-hydroxy-3-nonyl) adenine alone and in combination with 9-beta-d-arabinofuranosyladenine in treatment of systemic herpesvirus infections in mice. | although the antiviral activity of erythro-9-(2-hydroxy-3-nonyl)adenine, a potent adenosine deaminase inhibitor, against herpes simplex virus type 1 in cell culture was readily confirmed, the compound was found to be totally ineffective in the treatment of experimentally induced systemic herpes simplex virus type 1 infections in swiss mice. data were obtained, however, which clearly indicated that the antiviral potency of 9-beta-d-arabinofuranosyladenine in vivo could be enhanced by the co-admin ... | 1980 | 6255863 |
| selective inhibition of herpes simplex virus glycoprotein synthesis by a benz-amidinohydrazone derivative. | 1h-benz[f]indene-1.3(2h)dione-bis-amidinohydrazone (benzhydrazone) inhibited incorporation of 14c-glucosamine, 14c-fucose and 14c-mannose into glycoproteins of hep-2 cells infected with various strains of herpes simplex virus 1 (hsv-1) and impaired rna and protein synthesis to a low extent. these biochemical effects are very similar to those induced by glycosylation inhibitors such as tunicamycin, d-glucosamine and 2-deoxy-d-glucose. in contrast to these inhibitors, benzhydrazone reduced hsv gly ... | 1980 | 6255895 |
| immunogenicity of subviral herpes simplex virus type 1 preparation: protection of mice against intradermal challenge with type 1 and type 2 viruses. | an intradermal assay system for herpes simplex virus in the infected pinna (external ear) of mice was utilized for examining the immunogenicity of an experimental subviral herpes simplex type 1 virus vaccine. the administration of one dose of vaccine with adjuvant did not prevent the appearance of erythema; however, it markedly reduced the severity skin lesions induced by either type 1 or type 2 virus. in addition, neurological complications frequently seen in control mice were never observed in ... | 1980 | 6255896 |
| in vitro induction of human cell-mediated cytotoxicity directed against herpes simplex virus-infected cells: characterization of the effector lymphocyte. | normal human peripheral blood mononuclear leukocytes (pbml) were incubated (sensitized) in vitro for 6 days with herpes simplex virus type 1 (hsv-1)-infected cells. the sensitized pbml were then tested in a 5-hr 51cr-release microassay against uninfected or hsv-1-infected target cells. pbml subpopulation-depletion studies revealed that the effector cells were neither b cells nor monocytes, but that their activity was found both in the e-rosetting and the non-e-rosetting lymphocyte fractions. fur ... | 1980 | 6967905 |
| mechanism of recovery from systemic herpes simplex virus infection. i. comparative effectiveness of antibody and reconstitution of immune spleen cells on immunosuppressed mice. | the role of cellular immunity, humoral antibody, and interferon in recovery from primary systemic infection in mice due to herpes simplex virus type 1 (hsv-1) was studied. immunosuppression by three methods--each of which was sufficient to suppress humoral and cellular immunity--markedly potentiated primary systemic hsv-1 infection. immunosuppressed mice did not form neutralizing antibody to hsv-1, but passive transfer of physiologic amounts of neutralizing antibody as late as day 6 after infect ... | 1980 | 6967924 |
| cell-mediated immunity to herpes simplex virus: specificity of cytotoxic t cells. | this communication deals with the question of which of the viral antigens constitutes the targets for cytotoxic t lymphocytes (ctl) generated against herpes simplex virus type 1 (hsv-1). the approach used was, first, to compare cytotoxicity of ctl against target cells infected with virus in the presence of tunicamycin and 2-deoxy-d-glucose, which are known to inhibit glycoprotein synthesis, and second, to compare cytotoxicity of ctl against target cells infected with wild-type hsv-1 with that ag ... | 1980 | 6969228 |
| corrected splicing of a chicken ovalbumin gene transcript in mouse l cells. | mouse thymidine-kinase-negative l cells were transformed with a cloned chicken ovalbumin gene linked to the cloned thymidine kinase gene from herpes simplex virus type 1. most thymidine-kinase-positive clones contained one or more copies of the ovalbumin gene, which were stably maintained for at least 6 months during continuous culture under selective conditions. transcription of the ovalbumin gene was detected at a low level, producing rna molecules that were correctly spliced and polyadenylyla ... | 1980 | 6928676 |
| [prophylaxis of herpes simplex virus type 1 infection on guinea pig corneas by detergent-soluble extract (dse) of virus-infected cells: inhibitory effect of dse on the establishment of trigeminal ganglionic latency (author's transl)]. | 1980 | 6971568 | |
| returning to breast-feeding. | given the basic prejudice that breastfeeding is superior to bottle feeding, this article outlines proper behavior of clinicians in educating, supporting, and preparing mothers who want to breastfeed their infants. the discussion is broken down into clinician's behavior in the prenatal, intrapartum, and follow-up periods of breastfeeding practice. prenatally, the obstetrician should educate the mother and should examine the woman for contraindications to breastfeeding, taking care to correct th ... | 1980 | 7004694 |
| estimation of type-specific neutralizing antibody to herpes simplex virus type 2 in uterine cervical cancer patients by a new absorption method. | a simple method of estimating type-specific neutralizing antibody to type 2 herpes simplex virus (hsv-2) was devised with the use of the microneutralization system. serially diluted serum was mixed in the well with a constant amount of type 1 virus (hsv-1), and after 3 days' incubation at 37 c, the plate was irradiated with ultraviolet light. the absorbing hsv-1 consisted of culture fluid plus an extract of infected vero cells not especially concentrated. the well then received indicator hsv-1 o ... | 1980 | 7219207 |
| interferon in biopsy and autopsy specimens of brain. its presence in herpes simplex virus encephalitis. | quantitative virus and interferon titers were assayed in temporal lobe brain biopsy specimens (six patients); in various regions of the brain at autopsy (five of the six patients); and in serums and csf during courses of herpes simplex virus, type 1 (hsv-1) encephalitis. until the tenth day of neurologic disease, interferon (geometric mean titer, 25 units/ml) was present in each virus-positive brain biopsy specimen. the geometric mean hsv-1 titer at brain biopsy was 328 tissue culture dose (tcd) ... | 1980 | 7356411 |
| acyclovir topical therapy of cutaneous herpes simplex virus infection in guinea pigs. | the chemotherapeutic efficacy of acyclovir was evaluated by observing the potential of topical acyclovir to reduce the severity of herpes viral lesions and to control the multiplication of this virus in the experimentally induced primary cutaneous infection of guinea pigs. topical acyclovir showed a substantially high therapeutic efficacy when treatment was initiated after the development of clinically overt skin lesions. the therapeutic response was dose dependent and clearly evident even when ... | 1980 | 7377804 |
| a direct method for the preparation of 2-hydroxyethoxymethyl derivatives of guanine, adenine, and cytosine. | alkylation of 2-chloro-6-iodopurine with iodomethyl [(trimethylsilyl)oxy]ethyl ether at -63 degrees c and subsequent treatment of the 9-substituted chloroiodopurine with k2co3 in aqueous dioxane at 25 degrees c and then with nh3 under pressure at 150 degrees c provided 9-[(2-hydroxyethoxy)methyl]guanine (1a), a potent antiviral agent against herpes simplex virus type 1, in excellent yield. its monophosphate (1g), which is enzymatically produced from 1a in the virus-infected cell, was also synthe ... | 1980 | 7381859 |
| inhibition of cellular alpha and virally induced deoxyribonucleic acid polymerases by the triphosphate of acyclovir. | the effect of the triphosphate of 9-(2-hydroxyethoxymethyl)guanine (acyclovir, acycloguanosine) on cellular alpha deoxyribonucleic acid (dna) polymerases (dna nucleotidyltransferases), dna polymerases of several members of the herpes group, vaccinia virus dna polymerase, and friend leukemia virus ribonucleic acid-dependent dna polymerase was examined. several viruses, which were found to be susceptible to acyclovir, were found to induce dna polymerases which were sensitive to acyclovir triphosph ... | 1980 | 7192534 |
| [experimental endophthalmitis induced by herpes simplex virus. 1. intravitreal inoculation (author's transl)]. | 1980 | 7196141 | |
| relationship of antibody to outcome in neonatal herpes simplex virus infections. | neutralizing antibody titers to herpes simplex virus type 1 (hsv-1) and hsv-2 were measured at birth in normal infants and uninfected infants of mothers with genital hsv infections during pregnancy and at the onset of infection in 5 infants with mild infections and 11 infants with severe infections. thirty-eight percent of premature and 29% of term infants had neutralization titers of <1:5. high titers ([unk]1:40) were found in 55% of infants of mothers with primary infections during pregnancy a ... | 1980 | 7216423 |
| immune response to herpes simplex virus infections: virus-specific antibodies in sera from patients with recurrent facial infections. | radioimmunoprecipitation assays were used to identify antibodies against a number of herpes simplex virus type 1-specific antigens in serum samples from individuals with recurrent facial herpes virus infections and from seropositive individuals without recurrent infections. individuals with recurrent infections contributed three sequential serum samples each: immediately after the appearance of lesions, 3 weeks later, and 3 months later. antibodies against at least 18 viral polypeptides were pre ... | 1981 | 7216464 |
| immune responses to labial infection of balb/c mice with herpes simplex virus type 1. | the kinetics of appearance of five humoral antibody responses (micro-neutralization assay [nt], complement fixation [cf], enzyme-linked immunosorbent assay [elisa], radioimmunoassay [ria], antibody-dependent cell-mediated cytotoxicity [adcc]), were compared during labial infection of balb/c mice with herpes simplex virus type 1 strain patton. the elisa/ria antibody responses were present in most mice by day 5 after infection, at the beginning of the herpetic lip lesions; antibody effective in ad ... | 1981 | 7216485 |
| antiviral activity of win 41258-3, a pyrazole compound, against herpes simplex virus in mouse genital infection and in guinea pig skin infection. | win 41258-3 (4-[6-(2-chloro-4-methoxyphenoxy)hexyl]-3,5-diethyl-1h-pyrazole methane sulfonate) has in vitro and in vivo activity against herpes simplex virus types 1 and 2. in cell culture, a concentration of 2 microgram/ml produced a greater than 50% inhibition of plaque formation of herpes simplex virus type 2, and 3 microgram/ml produced a 100% reduction of herpes simplex virus type 1. win 41258-3 was effective against herpes simplex virus types 1 and 2 in mouse genital infection after intrav ... | 1981 | 7247370 |
| genital herpes simplex--concepts and treatment. | genital herpes simplex is an increasingly common venereal disease caused by either herpes simplex virus type 1 or 2. the disease can be manifested in a primary and/or recurrent form, the clinical features and complications of which are reviewed. potential problems include psychosocial difficulties, neonatal herpes simplex infections, and the possibility of an oncogenic role in cervical cancer. diagnostic modalities, prophylactic measures, and management technics are discussed. | 1981 | 7263972 |
| in vitro induction of human cell-mediated cytotoxicity directed against herpes simplex virus-infected cells. kinetics in normal donors and patients with recurrent herpes labialis. | in order to analyse cellular cytotoxic mechanisms involved in herpes virus infections, human peripheral blood mononuclear leukocytes (pbml) were incubated (sensitized) for up to 7 days with uninfected or herpes simplex virus type 1 (hsv-1)-infected cells. the sensitized pbml were then tested in a 5-hr 51cr-release microassay against uninfected, hsv-1-infected, or cytomegalovirus (cmv)-infected target cells. pbml from normal donors, sensitized with hsv-1 infected cells, exhibited strong cytotoxic ... | 1981 | 7265173 |
| herpes simplex type 1 retinitis in an adult with systemic herpes zoster. | a 72-year-old man developed bullous skin lesions two months before he was discovered to have malignant lymphoma. herpes zoster virus grew from the skin bullae. he developed encephalitis, keratitis in the left eye, and bilateral retinitis 18 months later. herpes simplex virus type 1 grew from cultures of the eyelid vesicles and corneal scrapings from the left eye. the patient died two years after the diagnosis of malignant lymphoma. virus particles believed to be herpes simplex virus were demonst ... | 1981 | 7270636 |
| ac2idu, bvdu, and thymine arabinoside therapy in experimental herpes keratitis. | the therapeutic efficacy of three new antiviral agents-5-iodo-3',5'-diacetyl-2'-deoxyuridine (diacetylidoxuridine, 1% ac2idu), e-5-(2-bromovinyl)-2'-deoxyuridine (0.25% bvdu), and 3% thymine arabinoside-is compared with available antivirals in an experimental model of herpes simplex virus type 1 (hsv-1) keratitis in new zealand white rabbits. compared with placebo, ac2idu significantly reduced ulcerative keratitis on days 4 through 8 after inoculation with virus and iritis on day 8 after inocula ... | 1981 | 7283815 |
| herpesvirus type 2 in adenocarcinoma of the uterine cervix: a possible association. | sera from 16 jewish patients with histologically confirmed adenocarcinoma of the uterine cervix (auc) and 32 control subjects matched by age and ethnic origin were examined for herpesvirus type 1 (hsv-1) and type 2 (hsv-2) neutralizing antibodies. titers were determined by means of the quantitative plaque reduction neutralization test on vero cell monolayers, using hsv-1 strain vr3 and hsv-2 strain. significantly increased levels of antibodies against both virus strains were found in the auc pat ... | 1981 | 7284955 |
| organization of the nucleolar rnp components in cultured rabbit fibroblasts following mild loosening of the nuclear content. | the organization of the ribonucleoprotein (rnp) components of the nucleolus was studied in ultrathin sections by a recent method of mild loosening of the nuclear content. this investigation was carried out in cultured rabbit fibroblasts in which the nucleoli contain clearly separated fibrillar and granular rnp components. in addition, the demarcation between these two particulate components was accentuated by infection with herpes simplex type 1 virus (hsv1), by recovery at 37 degrees c after a ... | 1981 | 7333285 |
| effects of adenosine monophosphate on the reactivation of latent herpes simplex virus type 1 infections of mice. | adenosine monophosphate pretreatment of mice with latent herpes simplex virus type 1 infections significantly reduced the rate of reactivation of latent virus. adenosine monophosphate pretreatment did not, however, eradicate latent virus. | 1981 | 7342880 |
| detection of an immediate early herpes simplex virus type 1 polypeptide in trigeminal ganglia from latently infected animals. | in this study, trigeminal sensory ganglia from animals with acute herpes simplex virus, type 1 (hsv-1) infection were compared to those with a latent infection for the expression of hsv-specific antigens. by the indirect immunofluorescence assay, antisera to an immediate early polypeptide of molecular weight 175,000, designated vp175 or icp4, and a hyperimmune antiserum to hsv-1 were used to determine whether early viral polypeptides were being expressed in neurons during the latent stage of inf ... | 1981 | 7037648 |
| herpes simplex virus type 2 and human cervical cancer: relationship between cellular and immune assays for the detection of previous infection. | women with invasive carcinoma of the cervix were examined for evidence of previous infection with herpes simplex virus (hsv) type 2 (hsv-2) by three assays: assay 1 measured the ratio of lymphocyte blastogenesis to herpes simplex virus type 1 (hsv-1) and hsv-2 antigens; assay 2 measured the ratio of antibody titer to surface antigens of hsv-1-infected and hsv-2-infected cells; assay 3 measured the titer of type-specific antibody to hsv-1 and hsv-2, including the percentage of hsv-2 type-specific ... | 1981 | 6941038 |
| cell-mediated immunity to herpes simplex virus: induction of cytotoxic t lymphocyte responses by viral antigens incorporated into liposomes. | the immunogenicity of inactivated herpes simplex virus type 1 (hsv-1) antigens incorporated into liposomes was measured by their ability to induce secondary anti-hsv-1 specific cytotoxic t lymphocyte (ctl) responses in splenocyte cultures from virus-primed mice. such virus-specific ctl could be induced provided the liposomes contained virus along with plasma membrane antigen of the same h-2 type as that of the virus-primed responser cells. responses did not occur in cultures stimulated with lipo ... | 1981 | 6969745 |
| the involvement of herpes simplex virus type 1 glycoproteins in cell-mediated immunity. | the participation of herpes simplex virus (hsv) glycoproteins in t cell-mediated lysis of hsv-infected syngeneic target cells was examined by using a temperature sensitive (ts) mutant defective in glycoprotein synthesis at the nonpermissive temperature (39 degrees c), and 2-deoxy-d-glucose and tunicamycin, known inhibitors of both hsv replication and glycoprotein synthesis in hsv-infected cells. lymphocytes cytotoxic for hsv-infected cells lysed c57bl/6 wt-3 cells infected with the mutant, ts a1 ... | 1981 | 6971309 |
| evaluation of 2,2'-anhydro-1-(3'-ok-acetyl-beta-d-arabinofuranosyl)-5-iodocytosine hydrochloride and related compounds as antineoplastic and antiviral agents. | 2,2'-anhydro-1-(3'-o-acetyl-beta-d-arabinofuranosyl)-5-iodocytosine (anhydro-araic 3'-acetate), previously synthesized and isolated as a crude product by moffatt and his coworkers, was purified and characterized. the availability of pure anhydro-araic 3'-acetate made possible a comparative study of the antineoplastic, antiviral and biochemical potencies of anhydro-araic 3'-acetate with the structurally related agents 2,2'-anhydro-1-(3'-o-acetyl-beta-d-arabinofuranosyl)cytosine (anhydro-arac 3'-a ... | 1981 | 6257405 |
| induction of uracil-dna glycosylase and dutp nucleotidohydrolase activity in herpes simplex virus-infected human cells. | hela bu cells infected with either the type 1 or the type 2 forms of herpes simplex virus show an increase in the activities of uracil-dna glycosylase and dutp nucleotidohydrolase. under optimal conditions, uracil-dna glycosylase activity increases approximately 40-fold in hsv type 2-infected cells. in herpes simplex virus (hsv) type 1-infected cells, uracil-dna glycosylase activity increases only 6-fold. at a kcl concentration of 100 mm, uracil-dna glycosylase derived from hsv type 2-infected c ... | 1981 | 6115860 |
| herpes simplex virus types 1 and 2 and multiple sclerosis. | a hypothesis is proposed which states that multiple sclerosis (ms) is caused by herpes simplex virus type 2 (hsv-2) in persons lacking herpes simplex virus type 1(hsv-1) immunity. it is examined by comparing the epidemiology of these viruses with that of ms. the age at which hsv-2 infections are first detected, as well as the age of peak incidence are similar to those for ms. if age-specific hsv-1 immunity data from various places is compared, an inverse relationship of immunity levels with lati ... | 1981 | 6116906 |
| antiviral activity of substituted 6-methylmercaptopyrazolo(3,4-d)pyrimidines and their ribosides. | the antiviral activities of pyrazolo(3,4-d)pyrimidines and their nucleosides, divided into 7 groups based on the substituent in position 4 (mercapto-, methylmercapto-, amino-, hydrazine-, dialkyl amino-, oxy-, and methoxyderivatives), were studied against herpes simplex virus type 1 (hsv 1) and vaccinia virus. numerous compounds inhibited the virus yields by 1-4 log cpd50. most antiviral compounds inhibited hsv 1 more than vaccinia virus. only methylmercapto- derivatives inhibited both viruses a ... | 1981 | 6118054 |
| comparison of the effects of arabinosyladenine, arabinosylhypoxanthine, and arabinosyladenine 5'-monophosphate against herpes simplex virus, varicella-zoster virus, and cytomegalovirus with their effects on cellular deoxyribonucleic acid synthesis. | in a single line of human foreskin fibroblasts, minimum inhibitory concentrations (mics) and the minimum intracellular virus inactivation concentrations (miics) of arabinosyladenine, arabinosylhypoxanthine, and arabinosyladenine 5'-monophosphate were assayed for a number of recent isolates of herpes simplex virus types 1 and 2 (hsv-1, hsv-2), varicella-zoster virus (vzv), and cytomegalovirus (cmv). (the term miic is used here to describe the selective qualitative intracellular inhibition of the ... | 1981 | 6166244 |
| anticomplement immunofluorescence for the titration of antibody to varicella-zoster virus. | anticomplement immunofluorescence (acif) was tested for its use for the titration of antibody against varicella-zoster virus (vzv). acif antibody responses of patients with vzv infection were specific for vzv antigen and heterotypic responses to herpes simplex virus type-1 and cytomegalovirus antigens were not observed. comparative studies of acif, membrane immunofluorescence (mif) and indirect immunofluorescence (if), using acetone-fixed antigen, were carried out with nonimmune sera and convale ... | 1981 | 6166838 |
| herpes simplex virus type i dna polymerase. kinetic properties of the associated 3'-5' exonuclease activity and its role in araamp incorporation. | an exonuclease activity copurified with herpes simplex virus type i (hsv-1) dna polymerase through dna-cellulose column chromatography and comigrated with dna polymerase activity on nondenaturing gel electrophoresis at varied polyacrylamide concentrations. a gapped duplex dna was the preferred substrate for this exonuclease activity since the hydrolytic activity on this type of dna was much greater than the hydrolysis of either native or heat-denatured dna. using 3'-terminally labeled activated ... | 1981 | 6167579 |
| experimental infection of inbred mice with herpes simplex virus type 1. i. investigation of humoral and cellular immunity and of interferon induction. | considerable differences exist in the lethality of herpes simplex virus type 1 (hsv-1) after intraperitoneal (i.p.) infection in different inbred strains of mice. in this study humoral and cellular immunity and interferon production were compared in resistant and susceptible mice. the serum igg response, as determined by an enzyme-linked immunosorbent assay (elisa), was the same in different strains of mice. there was also no difference in neutralizing antibodies between resistant c57bl/6 and su ... | 1981 | 6168735 |
| resistance to herpes simplex virus - type 1 (hsv-1). | 1981 | 6171384 | |
| herpes simplex virus type 1 and 2 intracellular p40: type-specific and cross-reactive antigenic determinants on peptides generated by partial proteolysis. | intracellular p40 is a class of protein ranging in molecular weight from 39,000 to 45,000 that is immunoprecipitated from herpes simplex virus type 1 (hsv-1)- and hsv-2-infected cell extracts by mouse monoclonal antibodies or guinea pig antisera against hsv-1 and hsv-2 nucleocapsid p40. analysis by a two-dimensional gel system showed that hsv-1 and hsv-2 intracellular p40 each consisted of three major components. however, these hsv-1 and hsv-2 proteins differed in charge and size. analysis of st ... | 1981 | 6172597 |
| inhibition of cellular and virus-associated nucleotide polymerases by, and anti-herpes simplex virus activity of, streptovaricin derivatives. | fourteen streptovaricin derivatives were tested for inhibition of cellular nucleotide polymerases (deoxyribonucleic acid polymerases alpha, beta, and gamma, terminal deoxynucleotidyltransferase [tdt], and ribonucleic acid polymerase ii), simian sarcoma virus deoxyribonucleic acid polymerase, and herpes simplex virus type 1-induced deoxyribonucleic acid polymerase (hsv-dp). three compounds (strep-tovadienal c, prestreptovarone, and streptoval fc) preferentially inhibited tdt and hsv-dp over the o ... | 1981 | 6119956 |
| characteristics of chromatin preparations from herpes simplex virus infected cells. | chromatin isolated from herpes simplex virus type 1-infected baby hamster kidney cells contains a number of tightly associated virus-induced polypeptides. a subset of these proteins bind to immobilized dna in vitro (vmw 175, 155, 130, 63, 43, 38/39). virus-induced polypeptides extractable with acid from infected cell chromatin include vmw 155, the major capsid protein of herpes simplex virus type 1 virions, and vmw 63 and 38/39 which are heterogeneous with respect to charge and are phosphorylate ... | 1981 | 6266485 |
| formation of infectious progeny virus after insertion of herpes simplex thymidine kinase gene into dna of an avian retrovirus. | we have prepared several infectious stocks of an avian retrovirus, spleen necrosis virus, containing the herpes simplex virus type 1 thymidine kinase (tk) gene. the viruses were produced after cotransfection of chicken cells with dna from recombinants between cloned spleen necrosis virus and tk dnas and dna of cloned reticuloendotheliosis virus strain a. removal of sequences in the tk gene for the end of tk mrna increased a thousand fold the yield of infectious recombinant virus. infection of ch ... | 1981 | 6276009 |
| molecular genetics of herpes simplex virus. v. characterization of a mutant defective in ability to form plaques at low temperatures and in a viral fraction which prevents accumulation of coreless capsids at nuclear pores late in infection. | in herpes simplex virus-infected cells, coreless capsids accumulate at the nuclear pores soon after infection, but subsequently disappear, suggesting that, as in adenovirus-infected cells (s. dales and y. chardonnet, virology 56:465-483, 1973), the release of viral dna from nucleocapsids takes place at the nuclear pores. a nonlethal mutant, hsv-1(50b), produced by mutagenesis of hsv dna fragments and selected for delayed production of plaques at 31 degrees c, accumulated coreless capsids at the ... | 1981 | 6275122 |
| acyclovir-resistant mutants of herpes simplex virus type 1 express altered dna polymerase or reduced acyclovir phosphorylating activities. | the biochemical properties of four acyclovir-resistant mutants are described. two of these mutants, paar5 and bwr, specified nucleotidyl transferase (dna polymerase) activities which were less sensitive to inhibition by acyclovir triphosphate than their wild-type counterparts. another mutant, iudrr, exhibited reduced ability to phosphorylate acyclovir. the fourth mutant, acgr4, both induced an altered dna polymerase and failed to phosphorylate appreciable amounts of acyclovir. bwr, a new acyclov ... | 1981 | 6275127 |
| quantitation of herpes simplex virus type 1 shed in preocular tear film of rabbits treated with acyclovir. | the quantity and duration of herpes simplex virus type 1 shedding in the preocular tear film of rabbits were measured before, during, and after administration of acyclovir topically, intravenously, and by these routes. topical administration reduced shedding significantly, intravenous administration was without effect and in the combination regimen added nothing to the effectiveness of local application. the effects of acyclovir were temporary because there were significant increase in preocular ... | 1981 | 6275777 |
| virus inhibition assay for measurement of acyclovir levels in human plasma and urine. | a simple microplate virus inhibition assay which measures the levels of acyclovir in plasma and urine samples from patients was developed. the assay is based upon the inhibition of the cytopathic effect of herpes simplex virus type 1 on human fibroblast cells. the extent of inhibition of virus cytopathic effect, caused by dilutions of samples from patients, allowed determination of acyclovir concentrations to be made. the assay, which measured biological activity, could detect acyclovir levels o ... | 1981 | 6275786 |
| electrophysiological changes of hsv-1-infected dorsal root ganglia neurons in culture. | dissociated rat dorsal root ganglion cultures were infected with herpes simplex virus type 1 and studied electrophysiologically throughout the course of infection. alterations in the electrical parameters of the action potentials of these neurons occurred as early as 4 hours post infection. the maximum rate of rise, spike amplitude, and overshoot were decreased, and the full width at half maximum and resting membrane potential were increased. at 6 hours post infection there is a movement back to ... | 1981 | 6265604 |
| pyrimidine acyclic nucleosides, 1-[(2-hydroxyethoxy)methyl]pyrimidines as candidate antivirals. | a number of pyrimidine acyclic nucleosides were synthesized and tested for activity against herpes simplex virus type 1. synthesis of 1-[(2-hydroxyethoxy)methyl]cytosine (8) and 1-[(2-hydroxyethoxy)methyl]uracil (14) was accomplished in two or three steps from 2,4-diethoxypyrimidine and 2-(benzoyloxy)ethoxymethyl chloride. the 5-methyl (20), 5-(trifluoromethyl) (21), and 5-fluoro (22) analogues of 14 were available in two steps form the appropriate bis(trimethylsilyl)ated 5-substituted uracil an ... | 1981 | 6265637 |
| synthesis and antiviral properties of (z)-5-(2-bromovinyl)-2'-deoxyuridine. | (z)-5-(2-bromovinyl)uracil was obtained by photoisomerization of the e. isomer. similarly, (e)-5-(2-bromovinyl)-2'-deoxyuridine gave the required z isomer. (z)-5-(2-bromovinyl)-2'-deoxyuridine is much less active against herpes simplex virus type 1 (hsv-1) and somewhat less active against herpes simplex virus type 2 than is the e isomer. both isomers show similar activity against vaccinia virus. therefore, the highly potent and selective activity of (e)-5-(2-bromovinyl)-2'-deoxyuridine against h ... | 1981 | 6265638 |