Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
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| dna vaccine prime and recombinant fpv vaccine boost: an important candidate immunization strategy to control bluetongue virus type 1. | bluetongue virus (btv) is the causative agent of bluetongue (bt), an important sheep disease that caused great economic loss to the sheep industry. there are 26 btv serotypes based on the outer protein vp2. however, the serotypes btv-1 and btv-16 are the two most prevalent serotypes in china. vaccination is the most effective method of preventing viral infections. therefore, the need for an effective vaccine against btv is urgent. in this study, dna vaccines and recombinant fowlpox virus (rfpv) ... | 2015 | 26048472 |
| transient dominant selection for the modification and generation of recombinant infectious bronchitis coronaviruses. | we have developed a reverse genetics system for the avian coronavirus infectious bronchitis virus (ibv) in which a full-length cdna corresponding to the ibv genome is inserted into the vaccinia virus genome under the control of a t7 promoter sequence. vaccinia virus as a vector for the full-length ibv cdna has the advantage that modifications can be introduced into the ibv cdna using homologous recombination, a method frequently used to insert and delete sequences from the vaccinia virus genome. ... | 2015 | 25720477 |
| a prime/boost strategy using dna/fowlpox recombinants expressing the genetically attenuated e6 protein as a putative vaccine against hpv-16-associated cancers. | considering the high number of new cases of cervical cancer each year that are caused by human papilloma viruses (hpvs), the development of an effective vaccine for prevention and therapy of hpv-associated cancers, and in particular against the high-risk hpv-16 genotype, remains a priority. vaccines expressing the e6 and e7 proteins that are detectable in all hpv-positive pre-cancerous and cancer cells might support the treatment of hpv-related lesions and clear already established tumors. | 2015 | 25763880 |
| emergence of reticuloendotheliosis virus in pigeons in guangdong province, southern china. | reticuloendotheliosis virus (rev), an important immunosuppressive pathogen, has many hosts, including chickens, ducks, geese, turkeys, and wild birds. clinically, rev may lead to increased susceptibility to other pathogens, resulting in serious tissue damage (especially tumors) and the death of its host. in this study, we encountered a disease outbreak resulting in a large number of deaths of pigeons in guangdong province, southern china. histopathological analysis revealed apparent tumor-like l ... | 2016 | 27120185 |
| outbreaks of pox disease due to canarypox-like and fowlpox-like viruses in large-scale houbara bustard captive-breeding programmes, in morocco and the united arab emirates. | infectious diseases can be serious threats for the success of reinforcement programmes of endangered species. houbara bustard species (chlamydotis undulata and chlamydotis macqueenii), whose populations declined in the last decades, have been captive-bred for conservation purposes for more than 15 years in north africa and the middle east. field observations show that pox disease, caused by avipoxviruses (apv), regularly emerges in conservation projects of houbara bustard, despite a very strict ... | 2016 | 25651753 |
| rapid detection of contagious ecthyma by loop-mediated isothermal amplification and epidemiology in jilin province china. | the aim of this experiment was to develop a loop-mediated isothermal amplification (lamp) assay and to research the recent epidemiology of contagious ecthyma in jilin province, china, using the assay. a lamp assay targeting a highly conserved region of the f1l gene was developed to detect contagious ecthyma virus (cev). three hundred and sixty-five cases from 64 flocks in 9 different areas of jilin province, china, from 2011 to 2014 were tested using the lamp assay. the results showed that the s ... | 2016 | 26346652 |
| concurrent fowlpox and candidiasis diseases in backyard chickens with unusual pox lesions in the bursa of fabricius. | concurrent fowlpox and candidiasis diseases occurred in a backyard chicken flock. four deceased chickens (one nagoya breed and three white silkie chickens) were examined for diagnosis. at necropsy, white curd-like plaques were observed in the crop. fungal elements that stained positive for candida albicans with immunohistochemistry were distributed throughout the tongue, choanal mucosa, esophagus, and crop. typical fowlpox lesions, composed of proliferating epithelial cells with ballooning degen ... | 2016 | 27610735 |
| prime-boost therapeutic vaccination in mice with dna/dna or dna/fowlpox virus recombinants expressing the human papilloma virus type 16 e6 and e7 mutated proteins fused to the coat protein of potato virus x. | the therapeutic antitumor potency of a prime-boost vaccination strategy was explored, based on the mutated, nontransforming forms of the e6 (e6f47r) and e7 (e7ggg) oncogenes of human papilloma virus type 16 (hpv16), fused to the potato virus x (pvx) coat protein (cp) sequence. previous data showed that cp fusion improves the immunogenicity of tumor-associated antigens and may thus increase their efficacy. after verifying the correct expression of e6f47rcp and e7gggcp inserted into dna and fowlpo ... | 2016 | 27664839 |
| protection of mice against the highly pathogenic vvihd-j by dna and fowlpox recombinant vaccines, administered by electroporation and intranasal routes, correlates with serum neutralizing activity. | the control of smallpox was achieved using live vaccinia virus (vv) vaccine, which successfully eradicated the disease worldwide. as the variola virus no longer exists as a natural infection agent, mass vaccination was discontinued after 1980. however, emergence of smallpox outbreaks caused by accidental or deliberate release of variola virus has stimulated new research for second-generation vaccine development based on attenuated vv strains. considering the closely related animal poxviruses tha ... | 2016 | 27637905 |
| relationship between values of fowlpox elisa and the presence of "takes" after vaccination. | values from an elisa for evaluating the immune response induced by a commercial vaccine against fowlpox virus and the lesion at the site of inoculation (i.e., swelling of the skin or a pox where the vaccine was applied) were compared. the elisa was carried out with an antigen prepared by precipitation of a cell culture-propagated virus suspension with ammonium sulfate and concentration by centrifugation. a 0.1 m acetate buffer (ph 5) was used as the sensitizing solution for maximum specific bind ... | 2016 | 26953946 |
| current and future vaccines and vaccination strategies against infectious laryngotracheitis (ilt) respiratory disease of poultry. | infectious laryngotracheitis (ilt) is an economically important respiratory disease of poultry that affects the industry worldwide. vaccination is the principal tool in the control of the disease. two types of vaccines, live attenuated and recombinant viral vector, are commercially available. the first generation of gahv-1 vaccines available since the early 1960's are live viruses, attenuated by continuous passages in cell culture or embryos. these vaccines significantly reduce mortalities and, ... | 2016 | 28057380 |
| vector order determines protection against pathogenic simian immunodeficiency virus infection in a triple component vaccine by balancing cd4+and cd8+t-cell responses. | an effective aids vaccine should elicit strong humoral and cellular immune responses while maintaining low levels of cd4+t cell activation to avoid the generation of target cells for viral infection. the present study investigated two prime-boost regimens, both starting vaccination with single cycle immunodeficiency virus, followed by two mucosal boosts either with recombinant adenovirus (rad) or fowlpox virus (rfwpv) expressing sivmac239 or sivmac251gag/polandenvgenes, respectively. finally, ve ... | 2017 | 28904195 |
| recombinant fowlpox virus vector-based vaccines: expression kinetics, dissemination and safety profile following intranasal delivery. | we have previously established that mucosal uptake of recombinant fowlpox virus (rfpv) vaccines is far superior to other vector-based vaccines. specifically, intranasal priming with rfpv vaccines can recruit unique antigen-presenting cells, which induce excellent mucosal and systemic hiv-specific cd8+ t-cell immunity. in this study, we have for the first time investigated the in vivo dissemination, safety and expression kinetics of rfpv post intranasal delivery using recombinant viruses expressi ... | 2017 | 28056224 |
| multiple gene typing and phylogeny of avipoxvirus associated with cutaneous lesions in a stone curlew. | avipoxvirus (apv) infections have been observed in a wide variety of wild, captive and domestic avian hosts, recently including a range of island endemic and endangered species. however, not enough is known about genome diversity and phylogenetic relationships of apvs, as well as their host-range specificity. a wild stone curlew (burhinus oedicnemus) was recovered in sardinia (italy), showing large wart-like lesions and nodules on both legs and toes, which resulted positive to poxvirus by pcr. h ... | 2017 | 28054222 |
| reverse genetics system for the avian coronavirus infectious bronchitis virus. | we have developed a reverse genetics system for the avian coronavirus infectious bronchitis virus (ibv) in which a full-length cdna corresponding to the ibv genome is inserted into the vaccinia virus genome under the control of a t7 promoter sequence. vaccinia virus as a vector for the full-length ibv cdna has the advantage that modifications can be introduced into the ibv cdna using homologous recombination, a method frequently used to insert and delete sequences from the vaccinia virus genome. ... | 2017 | 28508215 |
| identification of clade e avipoxvirus, mozambique, 2016. | analysis of scab samples collected from poultry during outbreaks of fowlpox in mozambique in 2016 revealed the presence of clade e avipoxviruses. infected poultry were from flocks that had been vaccinated against fowlpox virus. these findings require urgent reevaluation of the vaccine formula and control strategies in this country. | 2017 | 28820373 |
| construction, selection and immunogenicity of recombinant fowlpox candidate vaccine co-expressing hiv-1 gag and gp145. | an hiv candidate vaccine for the chinese population was designed by constructing a recombinant fowlpox virus expressing hiv-1 gag and hiv gp145 proteins via homologous recombination and plaque screening using enhanced green fluorescent protein (egfp) as the reporter gene. egfp in the recombinant was then knocked out with the cre/loxp system yielding rfpvhg-hp, which was identified at the genomic, transcriptional and translational levels. the immunogenicity of rfpvhg-hp was analyzed by measuring ... | 2017 | 28611493 |
| structural basis of apoptosis inhibition by the fowlpox virus protein fpv039. | programmed cell death or apoptosis of infected host cells is an important defense mechanism in response to viral infections. this process is regulated by proapoptotic and prosurvival members of the b-cell lymphoma 2 (bcl-2) protein family. to counter premature death of a virus-infected cell, poxviruses use a range of different molecular strategies including the mimicry of prosurvival bcl-2 proteins. one such viral prosurvival protein is the fowlpox virus protein fpv039, which is a potent apoptos ... | 2017 | 28411240 |
| a novel rapid direct haemagglutination-inhibition assay for measurements of humoral immune response against non-haemagglutinatingfowlpox virusstrains in vaccinated chickens. | fowlpox (fp) is a serious disease in chickens caused byfowlpox virus(fpv). one method currently used to control fpv is vaccination followed by confirmation that antibody titres are protective using the indirect haemagglutination assay (iha). the direct haemagglutination inhibition (hi) assay is not done because most fpv strains do not agglutinate chicken red blood cells (rbcs). a novel fpv strain tpv-1 which agglutinates chicken rbcs was discovered recently and enabled a direct hi assay to be co ... | 2017 | 29226260 |
| isolation and molecular detection of avipoxvirus from field outbreaks in mymensingh, bangladesh. | the present study was performed for isolation, identification, and molecular detection of avipoxvirus [turkeypox virus (tpv), fowlpox virus (fpv), and pigeonpox virus (ppv)] from field outbreaks in some selected areas of mymensingh division, bangladesh. | 2019 | 31453171 |
| occurrence and phylogenetic analysis of avipoxvirus isolated from birds around jammu. | domesticated fowls, pigeons and turkey birds were screened for avipoxvirus infection from different areas in jammu region. based on typical pox lesions the overall occurrence in fowl was found to be 18.52%, 17.03% in pigeons and 57.14% in turkeys. mortality recorded in chicks was 41.96%, 45.36% in squabs, 100% in poults, and 20.00% in adult turkeys. both cutaneous and diphtheritic forms of the disease was observed of which the latter was particularly prevalent in young birds. one sample of putat ... | 2019 | 31179368 |
| characterization of fowlpox virus in chickens and bird-biting mosquitoes: a molecular approach to investigating avipoxvirus transmission. | avian pox is a highly contagious avian disease, yet relatively little is known about the epidemiology and transmission of avipoxviruses. using a molecular approach, we report evidence for a potential link between birds and field-caught mosquitoes in the transmission of fowlpox virus (fwpv) in singapore. comparison of fpv167 (p4b), fpv126 (vltf-1), fpv175-176 (a11r-a12l) and fpv140 (h3l) gene sequences revealed close relatedness between fwpv strains obtained from cutaneous lesions of a chicken an ... | 2019 | 30907721 |
| chicken embryonic-stem cells are permissive to poxvirus recombinant vaccine vectors. | the discovery of mammalian pluripotent embryonic stem cells (esc) has revolutionised cell research and regenerative medicine. more recently discovered chicken esc (cesc), though less intensively studied, are increasingly popular as vaccine substrates due to a dearth of avian cell lines. information on the comparative performance of cesc with common vaccine viruses is limited. using rna-sequencing, we compared cesc transcriptional programmes elicited by stimulation with chicken type i interferon ... | 2019 | 30897824 |
| efficacy of novel recombinant fowlpox vaccine against recent mexican h7n3 highly pathogenic avian influenza virus. | since 2012, h7n3 highly pathogenic avian influenza (hpai) has produced negative economic and animal welfare impacts on poultry in central mexico. in the present study, chickens were vaccinated with two different recombinant fowlpox virus vaccines (rfpv-h7/3002 with 2015 h7 hemagglutinin [ha] gene insert, and rfpv-h7/2155 with 2002 h7 ha gene insert), and were then challenged three weeks later with h7n3 hpai virus (a/chicken/jalisco/cpa-37905/2015). the rfpv-h7/3002 vaccine conferred 100% protect ... | 2019 | 30885512 |
| long-term protection against a virulent field isolate of infectious laryngotracheitis virus induced by inactivated, recombinant, and modified live virus vaccines in commercial layers. | infectious laryngotracheitis (ilt) is an acute respiratory disease of chickens controlled through vaccination with live-modified attenuated vaccines, the chicken embryo origin (ceo) vaccines and the tissue-culture origin (tco) vaccines. recently, novel recombinant vaccines have been developed using herpesvirus of turkey (hvt) and fowl pox virus (fpv) as vectors to express iltv immunogens for protection against ilt. the objective of this study was to assess the protection efficacy against ilt ind ... | 2019 | 30640536 |
| detection of fowlpox virus carrying distinct genome segments of reticuloendotheliosis virus. | fowlpox virus (fwpv), the type species of the genus avipoxvirus family poxviridae, is a large double-stranded dna virus that causes fowlpox in chickens and turkeys. notably, sequences of the avian retrovirus reticuloendotheliosis virus (rev) are frequently found integrated into the genome of fwpv. while some fwpv strains carry remnants of the rev long terminal repeats (ltrs), other strains have been shown to contain insertions of nearly the full-length rev provirus in their genome. in the presen ... | 2019 | 30359622 |
| detection and characterization of an avipoxvirus in a common buzzard ( buteo buteo) in italy using a multiple gene approach. | poxvirus infections have been reported in domestic, captive, and wild avian hosts including many raptor species. a wild common buzzard ( buteo buteo) admitted to a wildlife veterinary clinic in sardinia, italy, showed multiple, wart-like proliferative cutaneous lesions on both legs. histologically, there was ballooning degeneration and large intracytoplasmic inclusion bodies consistent with avipoxvirus (apv) infection. diagnosis was confirmed by pcr detecting apv genes: p4b (locus fpv167), p35 ( ... | 2019 | 29953311 |
| mucosal and systemic siv-specific cytotoxic cd4+ t cell hierarchy in protection following intranasal/intramuscular recombinant pox-viral vaccination of pigtail macaques. | a hiv vaccine that provides mucosal immunity is urgently needed. we evaluated an intranasal recombinant fowlpox virus (rfpv) priming vaccine followed by intramuscular modified vaccinia ankara (rmva) booster vaccine, both expressing siv antigens. the vaccination generated mucosal and systemic siv-specific cd4+ t cell mediated immunity and was associated with partial protection against high-dose intrarectal sivmac251 challenge in outbred pigtail macaques. three of 12 vaccinees were completely prot ... | 2019 | 30952887 |
| recombinant fowlpox virus expressing gb gene from predominantly epidemic infectious larygnotracheitis virus strain demonstrates better immune protection in spf chickens. | background: infectious laryngotracheitis (ilt) is a highly contagious acute respiratory disease of chickens. antigenic mutation of infectious laryngotracheitis virus (iltv) may result in a vaccination failure in the poultry industry and thus a protective vaccine against predominant iltv strains is highly desirable. methods: the full-length glycoprotein b (gb) gene of iltv with the two mutated synonymous sites of fowlpox virus (fpv) transcription termination signal sequence was cloned into the in ... | 2020 | 33105740 |
| construction and immune protection evaluation of recombinant virus expressing newcastle disease virus f protein by the largest intergenic region of fowlpox virus nx10. | fowlpox virus (fpv) is used as a vaccine vector to prevent diseases in poultry and mammals. the insertion site is considered as one of the main factors influencing foreign gene expression. therefore, the identification of insertion sites that can stably and efficiently express foreign genes is crucial for the construction of recombinant vaccines. in this study, we found that the insertion of foreign genes into orf054 and the orf161/orf162 intergenic region of the fpv genome did not affect replic ... | 2020 | 33009986 |
| molecular detection of reticuloendotheliosis virus 5' long terminal repeat integration in the genome of avipoxvirus field strains from different avian species in egypt. | avipoxviruses (apvs) are among the most complex viruses that infect a wide range of birds' species. the infection by apvs is often associated with breathing and swallowing difficulties, reduced growth, decreased egg production, and high mortalities in domestic poultry. in the present study, 200 cutaneous nodular samples were collected from different avian species (chicken, pigeon, turkey, and canary) suspected to be infected with apvs from dakahlia governorate, egypt. pooled samples (n = 40) wer ... | 2020 | 32878059 |
| avian leukosis virus contamination in live vaccines: a retrospective investigation in china. | avian leukosis (al) is one of the most pandemic immunosuppressive diseases and has been widely spread between 2006 and 2009 in china. the contamination of avian leukosis virus (alv) in attenuated vaccine is considered as one of the possible transmission routes of this disease. based on a retrospective survey of 918 batches of attenuated vaccine produced before 2010, three of them were identified as alv-positive and corresponding alv strains were successfully isolated from a live fowlpox virus va ... | 2020 | 32605749 |
| an atypical clinicopathological manifestation of fowlpox virus associated with reticuloendotheliosis virus in commercial laying hen flocks in brazil. | fowlpox (fp) is a common epitheliotropic disease in chickens that is usually controlled by live attenuated vaccines. however, there have been some reports of outbreaks of fp in recent years, even in vaccinated flocks, presenting as atypical lesions and feathering abnormalities in chickens. these findings can be associated with fowlpox virus (fpv) with the reticuloendotheliosis virus (rev) integrated into its genome. in the present study, outbreaks of atypical fp were explored in vaccinated comme ... | 2020 | 32519513 |
| prevalence of poultry red mite (dermanyssus gallinae) in korean layer farms and the presence of avian pathogens in the mite. | the poultry red mite, dermanyssus gallinae, is a blood-feeding parasite of layer hens and a potential vector of several avian infectious agents. high infestation with d. gallinae in layer farm buildings could result in economic losses, and the mites may act as a reservoir of avian pathogens within farms. this study aimed to assess the prevalence of d. gallinae in layer farm buildings in korea and to investigate avian pathogens in the collected mites. the mite samples were collected from 36 korea ... | 2020 | 32435982 |
| negligible effect of chicken cytokine il-12 integration into recombinant fowlpox viruses expressing avian influenza virus neuraminidase n1 on host cellular immune responses. | in comparison to the extensive characterization of haemagglutinin antibodies of avian influenza virus (aiv), the role of neuraminidase (na) as an immunogen is less well understood. this study describes the construction and cellular responses of recombinant fowlpox viruses (rfwpv) strain fp9, co-expressing na n1 gene of aiv a/chicken/malaysia/5858/2004, and chicken il-12 gene. our data shows that the n1 and il-12 proteins were successfully expressed from the recombinants with 48 kd and 70 kd mole ... | 2020 | 32427095 |
| first report on molecular characterization and phylogenetic analysis of reticuloendotheliosis virus in sudan. | the reticuloendotheliosis virus (rev) group of retroviruses infects a wide range of avian species, including chickens, turkeys, ducks, geese, quail, and prairie chickens. the infection can result in immunosuppression, runting syndrome, high mortality, acute reticular cell neoplasia, or t- and/or b-cell lymphoma. one pcr positive chicken spleen sample obtained in a previous study in addition to one marek's disease and three fowl pox (fp) vaccine samples were investigated in this study. a pcr assa ... | 2020 | 32040658 |
| protection of white leghorn chickens by recombinant fowlpox vector vaccine with an updated h5 insert against mexican h5n2 avian influenza viruses. | despite decades of vaccination, surveillance, and biosecurity measures, h5n2 low pathogenicity avian influenza (lpai) virus infections continue in mexico and neighboring countries. one explanation for tenacity of h5n2 lpai in mexico is the antigenic divergence of circulating field viruses compared to licensed vaccines due to antigenic drift. our phylogenetic analysis indicates that the h5n2 lpai viruses circulating in mexico and neighboring countries since 1994 have undergone antigenic drift awa ... | 2020 | 31862196 |
| whole genome sequencing of an avipoxvirus associated with infections in a group of aviary-housed snow buntings (plectrophenax nivalis). | avipoxvirus infections have been reported in both free-ranging and domestic birds worldwide. fowlpox and canarypox viruses belong to the genus avipoxvirus among the virus family poxviridae. they cause cutaneous lesions with proliferative growths on the unfeathered parts of the skin and/or diphtheritic lesions generally associated with necrosis in the upper respiratory and digestive tracts. in this study, a poxvirus has been identified in wild-caught snow buntings (plectrophenax nivalis) housed i ... | 2020 | 31926510 |
| modulation of early host innate immune response by an avipox vaccine virus' lateral body protein. | the avian pathogen fowlpox virus (fwpv) has been successfully used as a vaccine vector in poultry and humans, but relatively little is known about its ability to modulate host antiviral immune responses in these hosts, which are replication-permissive and nonpermissive, respectively. fwpv is highly resistant to avian type i interferon (ifn) and able to completely block the host ifn-response. microarray screening of host ifn-regulated gene expression in cells infected with 59 different, nonessent ... | 2020 | 33352813 |
| mucosal il-4r antagonist hiv vaccination with sosip-gp140 booster can induce high-quality cytotoxic cd4+/cd8+ t cells and humoral responses in macaques. | inducing humoral, cellular and mucosal immunity is likely to improve the effectiveness of hiv-1 vaccine strategies. here, we tested a vaccine regimen in pigtail macaques using an intranasal (i.n.) recombinant fowl pox virus (fpv)-gag pol env-il-4r antagonist prime, intramuscular (i.m.) recombinant modified vaccinia ankara virus (mva)-gag pol-il-4r antagonist boost followed by an i.m sosip-gp140 boost. the viral vector-expressed il-4r antagonist transiently inhibited il-4/il-13 signalling at the ... | 2020 | 33328567 |
| an unusual case of haemorrhagic bursa in a pullet flock associated with a genogroup 2 infectious bursal disease virus. | an unusual case of infectious bursal disease (ibd) was observed in eight-week-old commercial caged pullets. this flock (house 1) exhibited a one-day spike in mortality. on gross necropsy examination, enlarged, diffusely haemorrhagic bursas were observed. this lesion has been frequently described in cases of very virulent infectious bursal disease virus (vvibdv). a five-week-old caged pullet flock (house 2) in an adjacent building did not display haemorrhagic bursa lesions. microscopic examinatio ... | 2021 | 33823706 |
| research note: simultaneous detection of infectious laryngotracheitis virus, fowlpox virus, and reticuloendotheliosis virus in chicken specimens. | infectious laryngotracheitis (ilt), fowlpox (fp), and reticuloendotheliosis are important poultry diseases caused by gallid herpesvirus 1 (iltv), fowlpox virus (fwpv), and reticuloendotheliosis virus (rev), respectively. coinfections with iltv and fwpv occur naturally in chickens, and fp in its more virulent wet form is characterized by diphtheritic lesions and easily confused with ilt. moreover, the insertion of only partial rev-ltr or a nearly full-length rev into the fwpv genome, located betw ... | 2021 | 33647723 |
| chicken cgas senses fowlpox virus infection and regulates macrophage effector functions. | the anti-viral immune response is dependent on the ability of infected cells to sense foreign nucleic acids. in multiple species, the pattern recognition receptor (prr) cyclic gmp-amp synthase (cgas) senses viral dna as an essential component of the innate response. cgas initiates a range of signaling outputs that are dependent on generation of the second messenger cgamp that binds to the adaptor protein stimulator of interferon genes (sting). here we show that in chicken macrophages, the cgas/s ... | 2021 | 33633733 |