Publications

TitleAbstractYear(sorted ascending)
Filter
PMID
Filter
characterization of the ferret trb locus guided by v, d, j, and c gene expression analysis.the domestic ferret, mustela putorius furo, is an important mammalian animal model to study human respiratory infection. however, insufficient genomic annotation hampers detailed studies of ferret t cell responses. in this study, we analyzed the published t cell receptor beta (trb) locus and performed high-throughput sequencing (hts) of peripheral blood of four healthy adult ferrets to identify expressed v, d, j, and c genes. the hts data is used as a guide to manually curate the expressed v, d, ...202031797007
blastocystis sp. subtype diversity in wild carnivore species from spain.the occurrence and molecular diversity of the stramenopile eukaryote blastocystis sp. was investigated by pcr and sequencing (sanger and ngs) methods in 380 faecal specimens of free-living carnivores in spain. blastocystis sp. was confirmed in 1.6% (6/380) of the specimens analysed. two samples from a common genet and a fox were successfully subtyped as st7 by sanger. using ngs, st14 was found in a fox and a european polecat, st7 in a fox, and two additional foxes presented mixed infections of s ...202031691450
visual corticocortical inputs to ferret area 18.visual cortical areas in the adult mammalian brain are linked by a network of interareal feedforward and feedback circuits. we investigated the topography of feedback projections to ferret (mustela putorius furo) area 18 from extrastriate areas 19, 21, and ssy. our objective was to characterize the anatomical organization of the extrastriate feedback pool to area 18. we also wished to determine if feedback projections to area 18 share similar features as feedback projections to area 17. we injec ...202033117134
cryopreservation of ferret (mustela putorius furo) sperm collected by rectal massage and electroejaculation: comparison of a decelerating and an accelerating freezing rate protocol.the domestic ferret (mustela putorius furo) provides a good model for developing new reproductive technologies for use with threatened related species. such technologies could also be used in the reproductive management of this pet species. the present work reports an improved freezing protocol for ferret sperm. semen was collected by electroejaculation plus rectal massage (in an attempt to reduce the electrical stimulation necessary) from five adult male ferrets, and then subjected to one of tw ...202033040487
the amygdaloid body of two carnivore species: the feliform banded mongoose and the caniform domestic ferret.the current study provides an analysis of the cytoarchitecture, myeloarchitecture, and chemoarchitecture of the amygdaloid body of the banded mongoose (mungos mungo) and domestic ferret (mustela putorius furo). using architectural and immunohistochemical stains, we observe that the organization of the nuclear and cortical portions of the amygdaloid complex is very similar in both species. the one major difference is the presence of a cortex-amygdala transition zone observed in the domestic ferre ...202033009661
the diencephalon of two carnivore species: the feliform banded mongoose and the caniform domestic ferret.this study provides an analysis of the cytoarchitecture, myeloarchitecture, and chemoarchitecture of the diencephalon (dorsal thalamus, ventral thalamus, and epithalamus) of the banded mongoose (mungos mungo) and domestic ferret (mustela putorius furo). using architectural and immunohistochemical stains, we observe that the nuclear organization of the diencephalon is very similar in the two species, and similar to that reported in other carnivores, such as the domestic cat and dog. the same comp ...202032964417
treatment and follow-up of a domestic ferret (mustela putorius furo) with clinical leishmaniosis caused by leishmania infantum.leishmania infantum infection including treatment and follow up in domestic animals other than dogs and cats has not been described at this moment. this article describes the anti-leishmania treatment and follow-up of a ferret (mustela putorius furo) with leishmaniosis. a combined therapeutic protocol established for the patient, not yet approved for ferrets, was a combination of meglumine antimoniate plus allopurinol. a follow-up was established monthly during the first year in order to monitor ...202032862910
seasonal weight changes in laboratory ferrets.ferrets (mustela putorius furo) are a valuable animal model used in biomedical research. like many animals, ferrets undergo significant variation in body weight seasonally, affected by photoperiod, and these variations complicate the use weight as an indicator of health status. to overcome this requires a better understanding of these seasonal weight changes. we provide a normative weight data set for the female ferret accounting for seasonal changes, and also investigate the effect of fluid reg ...202032764762
visualization of the retina in intact eyes of mice and ferrets using a tissue clearing method.visualization of specific cells and structures in intact organs would greatly facilitate our knowledge about pathological changes; therefore, a tissue clearing method applicable to the intact eye may be valuable. here we report a novel imaging method for the retina using the hyperhydration-based tissue clearing technique cubic (clear, unobstructed brain/body imaging cocktails and computational analysis).202032704421
gene signatures of sars-cov/sars-cov-2-infected ferret lungs in short- and long-term models.coronaviruses (covs) consist of six strains, and the severe acute respiratory syndrome coronavirus (sars-cov), newly found coronavirus (sars-cov-2) has rapidly spread leading to a global outbreak. the ferret (mustela putorius furo) serves as a useful animal model for studying sars-cov/sars-cov-2 infection and developing therapeutic strategies. a holistic approach for distinguishing differences in gene signatures during disease progression is lacking. the present study discovered gene expression ...202032615317
persistent infection and pancytopenia associated with ferret systemic coronaviral disease in a domestic ferret.ferret systemic coronaviral disease (fscd) is a well-established cause of mortality in domestic ferrets. we describe herein novel findings in a case of fscd that was diagnosed and medically managed following virus detection by immunohistochemical (ihc) staining of surgical biopsy samples. hematologic changes in this ferret suggested spread of the virus to the bone marrow, which was confirmed by ihc staining of a postmortem sample. genotyping of the virus indicated that the virus grouped with alp ...202032589111
biochemical effects of routine gonadectomy on blood of domestic ferrets (mustela putorius furo).we studied domestic ferrets (mustela putorius furo) to evaluate the physiologic effects of routine surgery. standard plasma biochemistry panels and 1h-nmr spectroscopy of heparinized whole blood were performed on samples taken 24 h prior to and immediately after surgery from female and male ferrets undergoing routine gonadectomy. increases in plasma glucose, phosphorus, potassium, and creatine kinase concentrations associated with the duration of surgery were identified on plasma biochemistry pa ...202032586412
sequencing b cell receptors from ferrets (mustela putorius furo).the domestic ferret (mustela putorius furo) provides a critical animal model to study human respiratory diseases. however immunological insights are restricted due to a lack of ferret-specific reagents and limited genetic information about ferret b and t cell receptors. here, variable, diversity and joining genes within the ferret kappa, lambda and heavy chain immunoglobulin loci were annotated using available genomic information. a multiplex pcr approach was derived that facilitated the recover ...202032470013
sequencing smart: de novo sequencing and assembly approaches for a non-model mammal.whilst much sequencing effort has focused on key mammalian model organisms such as mouse and human, little is known about the relationship between genome sequencing techniques for non-model mammals and genome assembly quality. this is especially relevant to non-model mammals, where the samples to be sequenced are often degraded and of low quality. a key aspect when planning a genome project is the choice of sequencing data to generate. this decision is driven by several factors, including the bi ...202032396200
mapping the architecture of ferret brains at single-cell resolution.mapping the cytoarchitecture of the whole brain can reveal the organizational logic of neural systems. however, this remains a significant challenge, especially for gyrencephalic brains with a large volume. here we propose an integrated pipeline for generating a cytoarchitectonic atlas with single-cell resolution of the whole brain. to analyze a large-volume brain, we used a modified en-bloc nissl staining protocol to achieve uniform staining of large-scale brain specimens from ferret (mustela p ...202032351352
cystine urolithiasis in ferrets.urolithiasis in captive domestic ferrets has previously been predominantly struvite uroliths, although more recent laboratory submissions show a shift to predominantly cystine uroliths. genetic mutations for cystinuria have been identified in dogs, and it is suspected that underlying genetic mutations are partly responsible for this disease in ferrets. currently, surgery remains the only definitive treatment of cystine urolithiasis in ferrets, since dietary dissolution protocols have not been th ...202032327038
invasive mammalian predators habituate to and generalize avian prey cues: a mechanism for conserving native prey.invasive mammalian predators can cause the decline and extinction of vulnerable native species. many invasive mammalian predators are dietary generalists that hunt a variety of prey. these predators often rely upon olfaction when foraging, particularly at night. little is understood about how prey odor cues are used to inform foraging decisions. prey cues can vary spatially and temporally in their association with prey and can either reveal the location of prey or lead to unsuccessful foraging. ...202032573866
sars-cov-2 in fruit bats, ferrets, pigs, and chickens: an experimental transmission study.in december, 2019, a novel zoonotic severe acute respiratory syndrome-related coronavirus emerged in china. the novel severe acute respiratory syndrome coronavirus 2 (sars-cov-2) became pandemic within weeks and the number of human infections and severe cases is increasing. we aimed to investigate the susceptibilty of potential animal hosts and the risk of anthropozoonotic spill-over infections.202032838346
imgt® biocuration and comparative study of the t cell receptor beta locus of veterinary species based on homo sapiens trb.imgt®, the international immunogenetics information system® is the global reference in immunogenetics and immunoinformatics. by its creation in 1989 by marie-paule lefranc (université de montpellier and cnrs), imgt® marked the advent of immunoinformatics, which emerged at the interface between immunogenetics and bioinformatics. imgt® is specialized in the immunoglobulins (ig) or antibodies, t cell receptors (tr), major histocompatibility (mh), and proteins of the igsf and mhsf superfamilies. t c ...202032431713
natural sars-cov-2 infection in kept ferrets, spain.we found severe acute respiratory syndrome coronavirus 2 rna in 6 (8.4%) of 71 ferrets in central spain and isolated and sequenced virus from 1 oral and 1 rectal swab specimen. natural infection occurs in kept ferrets when virus circulation among humans is high. however, small ferret collections probably cannot maintain virus circulation.202134152974
host barriers to sars-cov-2 demonstrated by ferrets in a high-exposure domestic setting.ferrets (mustela putorius furo) are mustelids of special relevance to laboratory studies of respiratory viruses and have been shown to be susceptible to severe acute respiratory syndrome coronavirus 2 (sars-cov-2) infection and onward transmission. here, we report the results of a natural experiment where 29 ferrets in one home had prolonged, direct contact and constant environmental exposure to two humans with symptomatic disease, one of whom was confirmed positive for sars-cov-2. we observed n ...202133858941
coronaviruses associated with the superfamily musteloidea.among the animal superfamily musteloidea, which includes those commonly known as mustelids, naturally occurring and species-specific alphacoronavirus infections have been observed in both mink (mustela vison/neovison vison) and domestic ferrets (mustela putorius furo). ferret systemic coronavirus (frscv), in particular, has been associated with a rare but fatal systemic disease. in recent months, it has become apparent that both minks and ferrets are susceptible to severe acute respiratory syndr ...202133468694
in vitro characterisation of sars-cov-2 and susceptibility of domestic ferrets (mustela putorius furo).severe acute respiratory syndrome coronavirus-2 (sars-cov-2) is an emerging virus that has caused significant human morbidity and mortality since its detection in late 2019. with the rapid emergence has come an unprecedented programme of vaccine development with at least 300 candidates under development. ferrets have proven to be an appropriate animal model for testing safety and efficacy of sars-cov-2 vaccines due to quantifiable virus shedding in nasal washes and oral swabs. here, we outline o ...202133400387
sars-cov-2 seroprevalence in household domestic ferrets (mustela putorius furo).animal infections with sars-cov-2 have been reported in different countries and several animal species have been proven to be susceptible to infection with sars-cov-2 both naturally and by experimental infection. moreover, infections under natural conditions in more than 20 mink farms have been reported where humans could have been the source of infection for minks. however, little information is available about the susceptibility of pet animals under natural conditions and currently there is no ...202133801548
the ferret as a model for filovirus pathogenesis and countermeasure evaluation.the domestic ferret (mustela putorius furo) has long been a popular animal model for evaluating viral pathogenesis and transmission as well as the efficacy of candidate countermeasures. without question, the ferret has been most widely implemented for modeling respiratory viruses, particularly influenza viruses; however, in recent years, it has gained attention as a novel animal model for characterizing filovirus infections. although ferrets appear resistant to infection and disease caused by ma ...202133951727
mucosal challenge ferret models of ebola virus disease.recent studies have shown the domestic ferret (mustela putorius furo) to be a promising small animal model for the study of ebola virus (ebov) disease and medical countermeasure evaluation. to date, most studies have focused on traditional challenge routes, predominantly intramuscular and intranasal administration. here, we present results from a non-clinical pathogenicity study examining oronasal, oral, and ocular mucosal challenge routes in ferrets. animals were challenged with 1, 10, or 100 p ...202133806375
biodetection of a specific odor signature in mallard feces associated with infection by low pathogenic avian influenza a virus.outbreaks of avian influenza virus (aiv) infection included the spread of highly pathogenic aiv in commercial poultry and backyard flocks in the spring of 2015. this resulted in estimated losses of more than $8.5 million from federal government expenditures, $1.6 billion from direct losses to produces arising from destroyed turkey and chicken egg production, and economy-wide indirect costs of $3.3 billion from impacts on retailers and the food service industries. additionally, these outbreaks re ...202134038460
establishment of an induced pluripotent stem cell line from a female domestic ferret (mustela putorius furo) with an x chromosome instability.the domestic ferret (ferret; mustela putorius furo) is an important animal model for neuroscience and preclinical/veterinary medicine owing to its highly developed cerebral cortex and susceptibility to avian influenza and corona viruses. nevertheless, there is a lack of in vitro ferret models, since immortal cell lines including induced pluripotent stem cells (ipscs) of ferrets have been scarce. in this study, we established an ipsc line from ferret skin fibroblasts. the established ipsc line, f ...202134088013
Refresh
firstprev
1234567
nextlast
Displaying items 601 - 628 of 628