Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| effectiveness of soap formulations containing deet and permethrin as personal protection against outdoor mosquitoes in malaysia. | two soap formulations, both containing 20% deet and one each containing permethrin at 0.5 and 1.0%, respectively, were applied to exposed arms and legs of volunteers as personal protection against outdoor human biting mosquitoes in six locations on penang island, malaysia. the predominant mosquito species collected from these locations were aedes albopictus, mansonia uniformis, culex gelidus, anopheles lesteri and armigeres subalbatus. efficacy and residual effects up to 4 hours indicated good p ... | 1986 | 2906963 |
| multiplication of chikungunya virus in salivary glands of aedes albopictus (oahu strain) mosquitoes: an electron microscopic study. | aedes albopictus as well as aedes aegypti is an important vector of chikungunya and dengue viruses. electron microscopic observations on the salivary glands of ae. albopictus infected with chikungunya virus were performed in comparing with those of ae. aegypti infected with dengue virus. no virus budding from the cell surface of the chikungunya-infected mosquito's salivary glands was found as shown in dengue-infected ones, in contrast to the findings of the mammalian cells such as vero, kb, imr, ... | 1986 | 3599528 |
| marituba (bunyaviridae) virus replication in cultured aedes albopictus cells and in l-a9 cells. | the replication of marituba virus (bunyavirus genus, family bunyaviridae), was studied in aedes albopictus (mosquito) cells. infection of aedes albopictus cells with marituba virus was characterized by an initial acute phase of infection in which large amounts of virus were produced and further by a persistent phase of infection in which virus yield was much lower. no changes in host cell dna, rna and protein synthesis were observed in aedes albopictus cells infected with marituba virus. in cont ... | 1986 | 3729731 |
| aedes albopictus, an exotic mosquito vector in the united states. | 1986 | 3740685 | |
| sequence analysis of the e2 gene of a hyperglycosylated, host restricted mutant of sindbis virus and estimation of mutation rate from frequency of revertants. | svap15/21, a strain of sindbis virus (sv) derived from our standard laboratory strain of sv (svstd) after repeated passage on aedes albopictus cells, grows normally in mosquito cells but is host restricted (hr) in vertebrate cells. it is also temperature sensitive (ts) and produces pinpoint plaques on vertebrate cells (sp). e2 glycoprotein of svstd differs from that of the more widely used svhr (from which svstd was derived) by an additional (i.e., third) n-linked glycan. the e2 of svap15/21, in ... | 1986 | 3750843 |
| dominance of the cpe(+) phenotype in hybrid aedes albopictus cells infected with sindbis virus. | the effect of sindbis virus (sv) infection was analyzed in hybrid aedes albopictus cells formed by fusing ouabain-resistant cpe(+) cells to cpe(-) alpha-amanitin resistant cells. although the 24-h yields of virus from the parental cpe(+) and cpe(-) clones were similar, the rates of viral rna synthesis and virus release at early times post-infection were higher in the cpe(+) cells. in all eight hybrid clones studied, the cpe(+) phenotype was dominant. in addition, the kinetics of viral rna synthe ... | 1986 | 3765819 |
| titer distribution analysis of chikungunya virus in aedes albopictus (diptera: culicidae). | 1986 | 3950931 | |
| exclusion of superinfecting homologous virus by sindbis virus-infected aedes albopictus (mosquito) cells. | the infection of tissue-cultured aedes albopictus (mosquito) cells by an alphavirus ultimately results in a persistently infected cell population which can be maintained in the laboratory for years. one characteristic of this culture is that it will not support the replication of superinfecting homologous virus. we have previously shown that mosquito cells persistently infected with sindbis virus produce an antiviral agent which when applied to uninfected mosquito cells suppresses sindbis virus ... | 1986 | 3951024 |
| persistent infection of aedes albopictus c6/36 cells by bunyamwera virus. | two cell lines persistently infected with bunyamwera virus have been established from the c6/36 clone of aedes albopictus cells. the cells express bunyamwera virus antigens as detected by immunofluorescence and are resistant to superinfection with bunyamwera virus and other bunyaviruses, but not dugbe virus (nairovirus) nor vesicular stomatitis virus. the virus released from the persistently infected cells developed an altered cloudy or "bull's-eye" plaque morphology with increasing passage leve ... | 1986 | 3952984 |
| an outbreak of dengue virus at rio de janeiro--1986. | dengue virus type 1 has been isolated in aedes albopictus cell strain, from sera of patients living in the nova iguaçu county, by rio de janeiro. the clinical picture was characterized by fever, headache, retrobulbar pain, backache, pains in the muscles and the joints and prostration. studies in paired sera confirmed the presence of recent infection by dengue virus type 1. the outbreak reached adjacent areas, including rio de janeiro city (may, 1986). | 1986 | 3587006 |
| transovarial maintenance of san angelo virus in sequential generations of aedes albopictus. | an aedes albopictus line originally selected for efficient transovarial transmission (tot) of san angelo (sa) virus displayed a progressive decline in filial infection rate (fir) whenever artificial selection pressure was relaxed. this observation brought into question whether the efficiency of tot in this vector-virus model was sufficiently high to permit persistent vertical transmission over numerous freely reproducing generations. by tracing descendants of individual transovarially infected f ... | 1986 | 3082235 |
| chromosome banding homologies in three species of aedes (stegomyia). | the chromosome complements of the mosquitoes aedes aegypti, aedes mascarensis, and aedes albopictus, belonging to the subgenus stegomyia, gave a uniform response to the q-, h-, and r-banding techniques. of the three homomorphic chromosome pairs, only the shortest or sex pair (i) showed a consistent banding pattern. in the three species, a bright yellow intercalary band was present on one arm of both chromosomes of the sex pair after heat treatment and staining with acridine orange. the rest of t ... | 1986 | 2424581 |
| ribosome metabolism during the vitellogenic cycle of the mosquito, aedes aegypti. | ribosome accumulation and synthesis in the fat body of the mosquito aedes aegypti increased by approx. 4-fold during 18 h after the blood meal, consistent with the pattern of total rna accumulation during the synthetic phase of the vitellogenic cycle. by 24-30 h after the blood meal, the accumulated ribosomes began to be degraded, and the total rna content in the fat body eventually returned to previtellogenic levels. a method has been developed for isolation of ribosomal subunits from fat body, ... | 1987 | 3567223 |
| semliki forest virus-induced polykaryocyte formation is an atp-dependent event. | infection of aedes albopictus cells with semliki forest virus (sfv) leads to polykaryocyte formation below ph 6.2. this syncytium formation is accompanied by a decrease of the cellular atp level. addition of inhibitors of oxidative phosphorylation leads to a rapid, total depletion of atp in infected cells at ph 6 and results in an inhibition of polykaryocyte formation. however, when cells were exposed for only a few minutes to ph 6 in the presence of the inhibitors and then kept at ph 7.2, the a ... | 1987 | 3592981 |
| aedes albopictus, yellow fever, and the americas. | 1987 | 3800201 | |
| a novel method for the detection of early events in cell-cell fusion of semliki forest virus infected cells growing in monolayer cultures. | semliki forest virus infected aedes albopictus cells were used to investigate virus induced cell-cell fusion. it was shown by a novel method that cell-cell fusion was completed within approximately 5 minutes after triggering the fusion event by low ph. this method consists of fixing fusing cells with glutaraldehyde and microinjecting the highly fluorescent and rapidly diffusing dye lucifer yellow. in contrast, polykaryon formation, the usually used criterion to measure cell-cell fusion occurred ... | 1987 | 3606395 |
| susceptibility of aedes albopictus c6/36 cells to viral infection. | the susceptibility of the c6/36 clone of aedes albopictus monolayer cell cultures was determined with 46 prototype viruses passed through three subcultures. viral growth was confirmed by titration of the passage material in other susceptible host systems. nineteen viruses demonstrated good growth in c6/36 cells: coxsackievirus group a type 10 and group b types 2, 3, 4, and 5; enterovirus 69; mumps virus; poliovirus types 1 to 3; reovirus types 1 to 3; vaccinia virus; dengue virus type 2; eastern ... | 1987 | 3611315 |
| sexual transmission of dengue viruses by aedes albopictus. | male aedes albopictus experimentally infected with dengue virus types 1, 2, 3, or 4 transmitted their infection sexually to female ae. albopictus. such transmission was enhanced if the females had taken a bloodmeal 2 to 7 days prior to mating. male ae. albopictus also transmitted dengue virus vertically to their f1 progeny. infected progeny were found among those derived from eggs laid greater than or equal to 73 hr after mating but not among those derived from eggs laid prior to that time. this ... | 1987 | 3661831 |
| in vitro cultivation of rickettsia-like-organisms from glossina spp. | a method is described for the in vitro cultivation of the rickettsia-like-organisms (rlo) from glossina spp. which are believed to be associated with susceptibility to trypanosome infection. cultures of rlo were established by infecting a mosquito cell line (aedes albopictus) with haemolymph taken from teneral flies. rlo from nine species of glossina have been isolated and maintained in continuous culture using this technique. | 1987 | 3662675 |
| an enzyme immunoassay for dengue antibody using infected cultured mosquito cells as antigen. | a simple enzyme immunoassay (eia) for dengue virus antibody detection used infected tissue culture cells as antigen. c6/36 aedes albopictus cells infected with dengue virus, and uninfected control cells were fixed with 3.3% formalin. this technique eliminated microplate coating and laborious antigen preparation, and it facilitated rapid screening of large numbers of sera. formalin also inactivated dengue virus infectivity. microplates prepared by this technique could be stored at -20 or -70 degr ... | 1987 | 2890651 |
| detection of dengue virus rna using nucleic acid hybridization. | conditions for using slot-blot nucleic acid hybridization to quantitatively detect dengue-2 virus using a radiolabelled cdna probe, pvv17, were determined. as little as 11 plaque-forming units of virus were detected using a hybridization mixture without formamide and performing the test at 70 degrees c. while predominantly serotype-specific using stringent (65 degrees c) washing conditions, the probe detected all four dengue virus serotypes using astringent (28 degrees c) washing conditions. no ... | 1987 | 2881940 |
| effect of high temperature on aedes albopictus cells infected with mayaro virus. | the multiplication of mayaro virus in aedes albopictus cells was drastically inhibited after incubation at 37 degrees c. the effect of short-term exposure of infected cells to high temperatures (heat shock) produced a preferential translation of the heat shock messengers when compared to the viral mrnas. when cells were shifted back to 28 degrees c (the optimum growth temperature for aedes albopictus cells), preferential translation of viral mrna occurred. although the infected cells were progra ... | 1987 | 2843253 |
| vector competence of geographic strains of aedes albopictus and aedes polynesiensis and certain other aedes (stegomyia) mosquitoes for ross river virus. | the vector competence of geographic strains of aedes albopictus and ae. polynesiensis and fiji strains of ae. pseudoscutellaris and ae. aegypti was assessed for ross river (rr) virus, the etiologic agent of epidemic polyarthritis. strains of ae. polynesiensis from fiji, rarotonga, somoa and tahiti were the most susceptible to infection per os (mid50 less than or equal to 10(1.2) vero cell plaque-forming units [pfu]/blood meal). virus transmission data were variable, but all strains except the on ... | 1987 | 2849637 |
| vector competence of aedes albopictus from houston, texas, for dengue serotypes 1 to 4, yellow fever and ross river viruses. | a combination of virus infection and transmission experiments showed that a houston, texas strain of aedes albopictus is a competent vector for dengue (den), yellow fever (yf) and ross river (rr) viruses. however, at 14 days incubation, den virus infection rates in a puerto rican strain of aedes aegypti were significantly higher for each of the four den serotypes, except den-1, than in houston ae. albopictus fed simultaneously on the same virus suspensions. the degree of correlation between diss ... | 1987 | 2849638 |
| prolonged infection of human synovial cells with ross river virus. | primary cultures of human synovial cells shed infectious virus for 14 to 35 days following infection with isolates of ross river virus which had been passaged in the c6/36 line of aedes albopictus mosquito cells. no frank cytopathic effect was seen in infected synovial cells and they continued to replicate for the duration of the experiments. | 1987 | 2826654 |
| aedes albopictus in north america: probable introduction in used tires from northern asia. | north american strains of aedes albopictus, an asian mosquito recently introduced into the western hemisphere, exhibit photoperiodic sensitivity and cold-hardiness characteristics similar to strains originating from temperate zone asia. trade statistics for used tire imports, the most likely mode of introduction, also indicate a north asian origin. aedes albopictus, an important vector of dengue and a potential vector of many other arboviral diseases, may therefore have the capability of infesti ... | 1987 | 3576225 |
| a study on susceptibility of indonesia colonies of aedes aegypti and aedes albopictus mosquitoes to experimental infection with dengue type 3 and chikungunya viruses. | 1987 | 3586577 | |
| variation in the vector competence of geographic strains of aedes albopictus for dengue 1 virus. | eight geographic strains of aedes albopictus from asia and north america and one north american strain of aedes aegypti were tested for their vector competence with dengue 1 virus. three groups of ae. albopictus were established based on their vector competence: a) the oahu laboratory strain, b) the three malaysian strains, and c) the tokyo and three north american strains. the three north american strains were similar to the strain of ae. aegypti from houston, texas in their ability to transmit ... | 1987 | 3504922 |
| cytolytic activity and immunological similarity of the bacillus thuringiensis subsp. israelensis and bacillus thuringiensis subsp. morrisoni isolate pg-14 toxins. | the parasporal bodies of the mosquitocidal isolates of bacillus thuringiensis subsp. israelensis and b. thuringiensis subsp. morrisoni isolate pg-14 were compared with regard to their hemolytic and cytolytic activities and the immunological relatedness of the 28- and 65-kilodalton (kda) proteins that occur in both subspecies. the alkali-solubilized parasporal bodies of b. thuringiensis subsp. israelensis caused 50% lysis of human erythrocytes at 1.14 micrograms/ml, whereas those of b. thuringien ... | 1987 | 3300548 |
| dengue haemorrhagic fever in malaysia: a review. | the historical background, epidemiology and changing pattern of clinical disease as seen in malaysia is reviewed. the preliminary results of the longitudinal study of epidemiology of dengue in malaysia is also presented. studies led by rudnick et al. over some 18 years have established that the disease is endemically transmitted by both aedes aegypti and aedes albopictus causing illnesses ranging from mild febrile episodes through classical dengue fever, dengue haemorrhagic fever and the dengue ... | 1987 | 3324361 |
| characterization of the toxicity and cytopathic specificity of a cloned bacillus thuringiensis crystal protein using insect cell culture. | an insecticidal protein gene from bacillus thuringiensis var. aizawai was cloned in escherichia coli. the cloned gene expressed at a high level and the synthesized protein appeared as an insoluble, phase-bright inclusion in the cytoplasm. these inclusions were isolated by density gradient centrifugation, the isolated protein was activated in vitro by different proteolytic regimes and the toxicity of the resulting preparations was studied using insect cells grown in tissue culture. the inclusions ... | 1987 | 3330756 |
| the surface charge of l-a9 cells and aedes albopictus cells infected with marituba (bunyaviridae) virus. | the surface charge of marituba virus infected l-a9 cells and aedes albopictus cells was estimated by direct measurement of their electrophoretic mobilities. uninfected l-a9 cells and a. albopictus cells have mean electrophoretic mobilities of -1.083 microns/s x cm/v and -1.019 microns/s x cm/v, respectively. in marituba virus infected l-a9 cells a progressive decline in the electrophoretic mobility was observed. in contrast, in marituba virus infected a. albopictus cells the electrophoretic mobi ... | 1987 | 3111119 |
| update: aedes albopictus infestation--united states. | 1987 | 3119976 | |
| entry pathway of vesicular stomatitis virus into different host cells. | a biochemical and morphological investigation of the mechanism of entry of vesicular stomatitis virus (vsv) into host cells of mammalian (hela), avian (cer), piscine (epc) and arthropod (aedes albopictus) origin, is described. vsv was capable of infecting all cell lines tested by a endosome- and/or a lysosome-dependent step since ammonium chloride and amantadine blocked the early stages of infection. complement-dependent immune lysis of infected host cells provided evidence that in none of the f ... | 1987 | 3029282 |
| ultrastructural studies of kunjin virus-infected aedes albopictus cells. | ultrastructural changes in aedes albopictus cells infected with kunjin virus were characterized from 12 to 72 h post-infection. early in infection (16h), there were no prominent ultrastructural changes except for an increase in the number of vacuoles in the cytoplasm. as the infection progressed the rough endoplasmic reticulum appeared to lengthen and whorls of fibres were observed within some vacuoles. virus particles were observed in small numbers scattered in the cytoplasm between 24 to 30 h ... | 1987 | 3029292 |
| sindbis virus mutants resistant to mycophenolic acid and ribavirin. | previous work from this laboratory has demonstrated a correlation between the inhibition by ribavirin (rbv), mycophenolic acid (mpa), or 2-amino thiadiazole (tda) of sindbis virus replication in aedes albopictus mosquito cells and a reduction in cellular gtp levels. this reduction in gtp results from the inhibition by these drugs of inosine monophosphate dehydrogenase (impdh), the first enzyme specific for the de novo synthesis of gmp. by serial passage of sv in a. albopictus cells in the presen ... | 1987 | 3033882 |
| topological mapping of unique epitopes on the dengue-2 virus ns1 protein using monoclonal antibodies. | monoclonal antibodies were produced against two distinct thai dengue-2 (den-2) virus strains isolated in 1980 from dengue haemorrhagic fever patients. nine of 36 hybridomas produced monoclonal igg antibodies which reacted in radioimmune precipitation assays with the ns1 non-structural protein (42,000 mol. wt.) from den-2-infected c6/36 (aedes albopictus) cells. the virus specificity of ns1-reactive monoclonal antibodies was determined by indirect immunofluorescence assays using llc-mk2 cells inf ... | 1987 | 2434611 |
| aedes albopictus in chicago, illinois. | 1987 | 3504953 | |
| [a study on the characteristics of controlled-release tube and effectiveness of the slow release of phoxim on killing larvae of aedes albopictus]. | 1987 | 3449221 | |
| intraspecific and interspecific variation in the sequence and abundance of highly repeated dna among mosquitoes of the aedes albopictus subgroup. | interspecific variation in abundance of highly repeated dna sequences has been examined in three species of the aedes scutellaris and three species of the a. albopictus subgroup of the a. scutellaris group. sequences from a population of a. albopictus were hybridised with whole genomic content from other species and strains. copy number estimates were determined by dot-blot hybridisation. variation in sequence abundance between strains of a. albopictus was as great as between it and the other si ... | 1987 | 3597124 |
| estimation of the northern limits of distribution of aedes albopictus in north america. | 1987 | 3504917 | |
| genetic studies on two carboxylesterase loci in aedes albopictus. | two esterase loci, est-4 and est-5, in aedes albopictus encode carboxylesterases in 4th-instar larvae, pupae and adults. the electrophoretic bands migrate on agar gels toward the most anodal side, those of est-5 followed by those of est-4. linkage studies on the two loci revealed that they were arranged on linkage group 2 in the following order: est-4-(0.9 +/- 0.7 to 4.3 +/- 1.4 map units)-p(pigmented pupa)-(2 map units, as previously determined)-wb(white-body)-(18.2 +/- 2.6 to 21.0 +/- 1.8 map ... | 1987 | 3504903 |
| formation and maintenance of viroplasmic centers in tipula iridescent virus-infected mosquito cells with deranged cytoskeletons. | we have examined the role of cytoskeletal elements with respect to the formation and maintenance of viroplasmic centers (vcs) in tipula iridescent virus (tiv)-infected mosquito aedes albopictus (c6/36) cells. filamentous systems consisting of microtubules and microfilaments were detected by immunofluorescence microscopy. inoculation of cells with tiv resulted in an alteration of microtubule and microfilament organization whether or not vcs developed. the formation of short arrays of microtubules ... | 1987 | 3297704 |
| nucleoside and nucleobase transport and metabolism in wild type and nucleoside transport-deficient aedes albopictus cells. | nucleoside and nucleobase transport and metabolism were measured in atp-depleted and normal aedes albopictus mosquito cells (line c-7-10) by rapid kinetic techniques. the cells possess a facilitated diffusion system for nucleosides, which in its broad substrate specificity and kinetic properties resembles that present in many types of mammalian cells. the michaelis-menten constant for uridine transport at 28 degrees c is about 180 microm. however, the nucleoside transporter of the mosquito cells ... | 1987 | 3814594 |
| lactate dehydrogenase from cultured aedes albopictus cells: kinetic and isozyme analysis. | l(+) lactate dehydrogenase (ldh) activity from cultured cells of aedes albopictus was studied as a kinetic model of carbohydrate metabolism. enzyme kinetics were studied in the forward (lactate as substrate) and reverse (pyruvate as substrate) reactions and the apparent km values were obtained showing ldh higher affinity for pyruvate. the hill coefficient values for each substrate were similar and indicate the existence of only one binding site on the enzyme. isozyme analysis on cellulose-acetat ... | 1987 | 3454571 |
| control of aedes albopictus in the americas. | 1987 | 2450609 | |
| use of elevated temperatures to kill aedes albopictus and ae. aegypti. | 1988 | 3225579 | |
| effect of continuous heat stress on cell growth and protein synthesis in aedes albopictus. | aedes albopictus (clone c6/36) cells, which normally grow at 28 degrees c, were maintained at a supraoptimal temperature of 37 degrees c. the effect of continuous heat stress (37 degrees c) on cell growth was analyzed as were the modifications occurring with protein synthesis during short- and long-term heat stress. we observed that cells in lag or exponential growth phase, present inhibition of cell growth, and cells in the lag phase showed more sensitivity to death than cells growing exponenti ... | 1988 | 3192626 |
| aedes albopictus in florida. | 1988 | 3199126 | |
| susceptibility status of aedes albopictus to three topically applied adulticides. | the baseline susceptibility of aedes albopictus (sabah strain) to malathion was determined. both laboratory-colonized and field-collected ae. albopictus at harris county, tx are resistant to malathion but susceptible to scourge. the acute toxicity of bendiocarb to ae. albopictus is confounded by its rapid knockdown and delayed recovery syndrome of poisoning. | 1988 | 3199119 |
| aedes albopictus in the united states: rapid spread of a potential disease vector. | aedes albopictus, the asian "tiger mosquito," was found in houston, texas, in 1985. aedes albopictus is primarily a forest edge inhabiting species that has readily adapted to the container habitats produced by humans. although not yet incriminated in the spread of any disease in the americas, it has been repeatedly implicated in epidemic dengue and dengue hemorrhagic fever transmission in asia. it is a competent laboratory vector of la crosse, yellow fever and other viruses, and can transovarial ... | 1988 | 3058869 |
| [pathogenicity of spiroplasma sabaudiense (mollicute) for the cells (c6/36) of aedes albopictus (insecta: diptera) in vitro]. | an initial stock of spiroplasma sabaudiense had been maintained in cell-free medium for 2-3 years. subsequent passages in leibovitz's medium l-15 in the presence of aedes albopictus (c6/36) cells dramatically increased the pathogenicity of s. sabaudiense towards these cells. cytopathogenicity included production of syncytia, an increase in the number of mitochondria and alteration of their morphology, vacuolisation and a reduction in the rate of growth. cell lysis was associated with cytadsorpti ... | 1988 | 3064943 |
| an orbivirus of mosquitoes which induces co2 sensitivity in mosquitoes and is lethal for rabbits. | an orbivirus, jkt-7400, isolated from culex mosquitoes in indonesia, replicated to a high titer and induced cytopathic effects in aedes albopictus cell cultures. the virus produced lethal sensitivity to carbon dioxide in culex and aedes mosquitoes as well as in drosophila melanogaster fruit flies but was not the agent of the hereditary sensitivity to carbon dioxide previously described for culex quinquefasciatus. when injected intravenously in high doses, jkt-7400 virus was lethal for rabbits, a ... | 1988 | 3136255 |
| vector competence of a houston, texas strain of aedes albopictus for rift valley fever virus. | 1988 | 3193106 | |
| a survey of mosquitoes breeding in used tire stockpiles in connecticut. | a larval survey of nine used tire disposal sites in connecticut, was conducted from june through october 1987. no larvae of aedes albopictus were uncovered indicating that this mosquito is not yet established in the state. however, eight other mosquito species in four genera were found. the most abundant and widely distributed species found breeding in permanent tire dumps were ae. atropalpus, ae. triseriatus and culex restuans. culex pipiens was the second most common culex species followed by ... | 1988 | 3199116 |
| effects of dengue-1 infection in aedes albopictus on its susceptibility to malathion. | 1988 | 3199129 | |
| arboviruses recovered from sentinel cattle using several virus isolation methods. | a group of 20 sentinel steers was bled weekly for 5 months in 1986 and the blood samples were examined for arboviruses by inoculation firstly into embryonated chicken eggs (ece), baby mice, aedes albopictus cells and bhk21 monolayers. a second group of cattle was similarly examined for virus in 1987, except that baby mice were not used. viruses were recovered from 26% of the 878 weekly bleeds. the viruses identified consisted of 14 types belonging to the bluetongue, epizootic haemorrhagic diseas ... | 1988 | 3218074 |
| breeding structure of a colonizing species: aedes albopictus (skuse) in peninsular malaysia and borneo. | the mosquito, aedes albopictus, has recently become established in a number of cities throughout the united states. an initial survey of allozyme and genotypic frequencies in u.s. populations (black et al., 1988) revealed an extensive amount of local differentiation of populations and suggested that much genetic drift may have accompanied colonization. a study of gene flow was initiated in native habitats of ae. albopictus in malaysia to determine if the result observed in the u.s. was a consequ ... | 1988 | 3230033 |
| [variations in the phenotypic expression of the western nile fever virus after reproduction in two aedes albopictus skuse, 1895 cell lines]. | 1988 | 3231175 | |
| aedes albopictus mosquitoes introduced into brazil: vector competence for yellow fever and dengue viruses. | 1988 | 3232189 | |
| [arbovirus infections on the island of nosy-be; serologic and entomologic findings]. | since 1977, the pasteur institute of madagascar has been studying, during six surveys, the arboviruses of nosy-be area, in the north-west of madagascar. 47.2 p. 100 out of 271 human sera and 11.3 p. 100 out of 150 animal sera (mostly from lemurs), tested for antibodies to 16 arboviruses by the haemagglutination inhibition test, are positive. the results show an important prevalence of flaviviruses. west-nile and dengue 1 viruses were probably circulating some years before the surveys. antibodies ... | 1988 | 3242421 |
| detection of dengue virus type 2 in aedes albopictus by nucleic acid hybridization with strand-specific rna probes. | a molecular hybridization technique with radiolabeled, strand-specific rna probes was developed to detect dengue virus type 2 rna in pools of infected aedes albopictus mosquitoes. one infected mosquito in a pool of 25 could be detected, corresponding to a dengue virus type 2 titer of 2.75 log10 50% tissue culture infectious doses. | 1988 | 3281980 |
| leads from the mmwr. update: aedes albopictus infestation--united states. | 1988 | 3336132 | |
| svlm21, a mutant of sindbis virus able to grow in aedes albopictus cells in the absence of methionine, shows increased sensitivity to s-adenosylhomocysteine hydrolase inhibitors such as neplanocin a. | inhibition of s-adenosylhomocysteine (adohcy) hydrolase by compounds such as neplanocin a (npa) leads to the build-up of adohcy and the inhibition of methyltransferase enzymes. whether assayed by efficiency of plaquing or virus yield, svlm21, a mutant of sindbis virus resistant to methionine deprivation, was more sensitive to npa than was the standard virus (svstd) from which it was derived. for example, whereas 10 micrograms npa/ml depressed the yield of svlm21 by more than 30-fold, the yield o ... | 1988 | 3347999 |
| exposure of semliki forest virus-infected baby hamster kidney cells to low ph leads to a proton influx and a rapid depletion of intracellular atp which in turn prevents cell-cell fusion. brief report. | the conditions needed to trigger semliki forest virus (sfv) induced cell-cell fusion from within (ffwi) of baby hamster kidney (bhk) cells differs from some other cells (e.g., aedes albopictus cells). in contrast to such cells it is mandatory for bhk cells to be brought back to a neutral ph after a short exposure to mildly acidic ph for fusion to occur. this phenomenon can be explained by a sudden drop of the intracellular ph observed after clamping the extracellular ph below 6.2--the ph require ... | 1988 | 3355372 |
| breeding structure of a colonising species: aedes albopictus (skuse) in the united states. | allelic and genotypic frequencies were monitored at seven enzymatic loci in newly established aedes albopictus (skuse) populations in the united states. populations were sampled within the cities of new orleans, louisiana, houston, texas, three counties surrounding houston, memphis, tennessee, jacksonville, florida, and evansville and indianapolis, indiana. unique alleles and relatively high levels of heterozygosity were detected in new orleans, houston, surrounding counties and indianapolis sug ... | 1988 | 3366625 |
| effect of actinomycin d and cycloheximide on replication of sindbis virus in aedes albopictus (mosquito) cells. | production of sindbis virus in the presence of transcription and translation inhibitors was examined in three aedes albopictus cell lines. addition of cycloheximide to heat-resistant sindbis virus (svhr)-infected mosquito cells arrested viral rna synthesis completely, in contrast to the effects of this drug on virus-infected vertebrate cells. production of mature virus by both svhr (a variant commonly used as a wild-type virus) and sbamr (a mutant which is resistant to the effects of 18 h of pre ... | 1988 | 3392770 |
| survival of albumin, igg, igm, and complement (c3) in human blood after ingestion by aedes albopictus and phlebotomus papatasi. | the levels of albumin, igg, igm, and complement (c3) were determined by enzyme-linked immunosorbent assay in aedes albopictus and phlebotomus papatasi at regular intervals after feeding on human blood. albumin disappeared most rapidly; by 48 hr, detectable levels of albumin had decreased 100-fold. in contrast, igg and igm survived longer and were still detectable at low levels several days after ingestion. c3 was intermediate in its rate of degradation. in general, serum protein disappearance oc ... | 1988 | 3400799 |
| suppression of rna synthesis by a specific antiviral activity in sindbis virus-infected aedes albopictus cells. | an antiviral protein is released by mosquito cells persistently infected with sindbis virus. differences in both sensitivity to and production of this virus-specific activity were apparent in three independently produced aedes albopictus cell lines. this activity inhibits total viral rna synthesis in a time-dependent manner. the antiviral effect is maximally realized when cells are treated with the activity 48 h before infections. these data suggest that the antiviral activity induces an antivir ... | 1988 | 2824854 |
| investigation of the role of glycans for the biological activity of semliki forest virus grown in aedes albopictus cells using inhibitors of asparagine-linked oligosaccharides trimming. | the effects of n-linked-oligosaccharide-processing inhibitors on the formation of semliki forest virus (sfv) in c6/36 aedes albopictus cells were investigated. the glycosidase inhibitors deoxynojirimycin, deoxymannojirimycin and swainsonine prevented the formation of endo-h resistant structures, but had little effect on virus formation and on the biological activities of the virus. tunicamycin greatly inhibited virus formation, but had little effect on cell-cell fusion from within and the cleava ... | 1988 | 2973779 |
| ovitrap surveys of dengue vector mosquitoes in chiang mai, northern thailand: seasonal shifts in relative abundance of aedes albopictus and ae. aegypti. | aedes aegypti (l.) and aedes albopictus (skuse) were surveyed using ovitraps in residential areas in chiang mai, northern thailand. egg populations (both species inclusive) remained low in the dry season, but increased/decreased exponentially during the first/latter half of the rainy season, respectively. this seasonal pattern was similar to the seasonal distribution of dengue haemorrhagic fever cases in the area. during the dry season (november-march) ae.aegypti was dominant in urban and indoor ... | 1988 | 2980189 |
| growth of bluetongue and epizootic hemorrhagic disease of deer viruses in poikilothermic cell systems. | continuous cell lines from the ticks dermacentor variabilis, d. parumapertus, d. nitens, rhipicephalus sanguineus and r. appendiculatus, the mosquitoes aedes albopictus and culex quinquefasciatus and the african toad xenopus laevis were tested for their ability to replicate bluetongue (bt) and epizootic hemorrhagic disease of deer (ehd) viruses, and for their sensitivity as potential isolation systems. bt serotype 17 grew to peak titers of 10(4.5)-10(7.5) tcid50 ml-1 in all except one of the tic ... | 1988 | 2833002 |
| further observations on the mechanism of vertical transmission of flaviviruses by aedes mosquitoes. | as previously observed for dengue viruses, vertical (i.e., transgenerational) transmission of japanese and st. louis encephalitis viruses by aedes albopictus takes place when the fully formed egg, enclosed in the chorion, is oviposited. the demonstration of such a mechanism for three flaviviruses suggests that vertical transmission of all mosquito-borne flaviviruses may occur in the same manner. | 1988 | 2840833 |
| establishment and characterization of st louis encephalitis virus persistent infections in aedes and culex mosquito cell lines. | persistent infections with st louis encephalitis (sle) virus were established in three mosquito cell lines (aedes albopictus, a. dorsalis and culex tarsalis) and were maintained for over 2 years. all three persistently infected cell cultures shared two features: (i) no overt cytopathic effect and (ii) a relatively high proportion of cells infected (41 to 85%). the aedes persistently infected cultures were resistant to superinfection with the homologous virus but not heterologous viruses. two sig ... | 1988 | 2842432 |
| phenotypes of st louis encephalitis virus mutants produced in persistently infected mosquito cell cultures. | viral mutants that appeared during long-term persistent infections of mosquito cell cultures (aedes albopictus, a. dorsalis and culex tarsalis) with st louis encephalitis virus were characterized. evidence was obtained for the presence of temperature-sensitive mutants in the a. dorsalis and c. tarsalis persistently infected cultures, and small plaque mutants were predominant in all cultures except one of two cell cultures of c. tarsalis. virus from persistently infected a. albopictus cell cultur ... | 1988 | 2842433 |
| [arboviroses in the region of nosy-bé, madagascar. serologic and entomologic data]. | since 1977, the pasteur institute of madagascar has been studying, during six surveys, the arboviruses of nosy-be area, in the north-west of madagascar. 47.2% out of 271 human sera and 11.3% out of 151 sera of lemurs, tested for antibodies to 16 arboviruses by the haemagglutination inhibition test, are positive. the results show an important prevalence of flaviviruses. west nile and dengue 1 viruses were probably circulating some years before the surveys. antibodies against sindbis and rift vall ... | 1988 | 2900082 |
| importation of aedes albopictus and other exotic mosquito species into the united states in used tires from asia. | between may 18 and december 4, 1986, 79 seagoing containers and their contents of 22,051 used tires were inspected for adult mosquitoes as well as eggs and larvae. of the total inspected, 5,507 tires (25%) contained significant amounts of water. no adults or eggs were found. fifteen tires contained mosquito larvae that were identified as ae. albopictus, ae. togoi, culex pipiens complex, tripteroides bambusa and uranotaenia bimaculata. the infestation rate for all species was 6.8 infested tires p ... | 1988 | 2903907 |
| effect of cellular inhibitors on the infection of various susceptible cells with vesicular stomatitis virus. | the effect of some cellular function inhibitors on adsorption and successive events of penetration of vesicular stomatitis virus to phylogenetically unrelated permissive cells was investigated. treatment of hela, cer, epc and aedes albopictus cells with colcemide and cytochalasin d which affect cytoskeleton organization indicated that microfilaments but not microtubules were involved in the early events of vsv infection. inhibitors of oxidative phosphorylation such as dinitrophenol and sodium az ... | 1988 | 2906220 |
| detection of yellow fever virus nucleic acid in infected mosquitoes by rna:rna in situ hybridization. | an in situ hybridization technique was developed for the strand-specific detection of yellow fever virus (yfv) rna. an 35s-labeled, transcribed rna probe was used to detect positive-sense polarity yfv genomic rna in infected c6/36 (aedes albopictus) cells, dissected mosquito tissues, and sections of plastic-embedded, yfv-infected aedes aegypti mosquitoes. mosquito tissues fixed in buffered formalin retained morphological integrity. the low concentrations of probe used yielded high specific signa ... | 1988 | 2907776 |
| sv(lm21), a mutant of sindbis virus able to grow in aedes albopictus cells in the absence of methionine, shows increased sensitivity to s-adenosylhomocysteine hydrolase inhibitors such as neplanocin a. | inhibition of s-adenosylhomocysteine (adohcy) hydrolase by compounds such as neplanocin a (npa) leads to the build-up of adohcy and the inhibition of methyltransferase enzymes. whether assayed by efficiency of plaquing or virus yield, svlm21, a mutant of sindbis virus resistant to methionine deprivation, was more sensitive to npa than was the standard virus (svstd) from which it was derived. for example, whereas 10 microg npa/ml depressed the yield of svlm21 by more than 30-fold, the yield of sv ... | 1988 | 18644581 |
| the biology of aedes albopictus. | the biology of aedes albopictus is reviewed, with emphasis on studies of ecology and behavior. the following topics are discussed: distribution and taxonomy, genetics, medical importance, habitat, egg biology, larval biology, adult biology, competitive interactions, comparative studies with aedes aegypti, population dynamics, photoperiodism, and surveillance and control. | 1988 | 3068349 |
| saint lucia revisited. | the eastern caribbean island of saint lucia is now famous in parasitological history as the setting for a major programme of schistosomiasis control'. perhaps less well-known are the island's effective control of many intestinal parasites, and elimination of malaria, such that the current patterns of mortality and other demographic indicators now resemble those of industrialized countries. more recently, the island has become the focus for another community-based health programme as the caribbea ... | 1988 | 15463009 |
| the ribosomal genes of the mosquito, aedes aegypti. | the characterisation of the ribosomal genes of the mosquito, aedes aegypti, is described. preliminary experiments using a cloned drosophila ribosomal dna (rdna) repeat to probe southern transfers of ae. aegypti genomic dna has indicated that the rdna repeat of ae. aegypti is 9.0 kb in length and that individual rdna repeats exhibit a high degree of homogeneity with respect to length and the position of restriction enzyme recognition sites within the rdna. the preliminary mapping data together wi ... | 1989 | 2573528 |
| interspecific mating between louisiana strains of aedes albopictus and aedes aegypti in the field and laboratory. | interspecific mating between aedes albopictus males and ae. aegypti females was detected in the field using mark-release-recapture techniques. by 3 days after the release of virgin ae. aegypti females into a field site containing only ae. albopictus, 100% of the captured females were inseminated. laboratory investigations indicated that male ae. albopictus were very proficient at inseminating ae. aegypti females and that ae. aegypti males rarely inseminated ae. albopictus females, especially if ... | 1989 | 2584975 |
| infection of aedes albopictus by tolypocladium cylindrosporum. | the infection process of tolypocladium cylindrosporum in aedes albopictus is discussed. the integument is a common site of infection. spores of t. cylindrosporum are able to adhere to the exoskeleton and penetrate it. during its early stages of development the fungus is always surrounded by a thick bacterial muff. however these bacteria did not invade the host, and no bacterial cell was observed on the intact cuticle. conidia filtered by larvae rapidly filled the gut and most of them were hydrol ... | 1989 | 2915148 |
| the use of sulfite to study the mechanism of membrane fusion induced by e1 of semliki forest virus. | in this study we have used 35s-labeled sulfite to modify the disulfide bonds of the proteins at the cell surface of semliki forest-infected aedes albopictus cells before and after low ph treatment. this reagent specifically cleaves disulfide bonds and concomitantly reacts with the newly formed cysteines, thereby labeling the respective protein. treatment of the infected cells with sulfite led to inhibition of the fusion activity only when applied after low ph exposure. these cells exhibited subs ... | 1989 | 2909989 |
| effect of sindbis virus infection on induction of heat shock proteins in aedes albopictus cells. | when aedes albopictus cells (clone c7) were infected with sindbis virus, the production of cytopathic effect cpe depended largely on the conditions under which the cells were cultured. we observed marked inhibition of cellular rna and protein synthesis, as well as a loss of the ability to induce heat shock proteins, e.g., hsp70, under conditions which led to cytopathic effect. infected cells in which heat shock proteins could no longer be induced contained much lower amounts of hsp70 mrna after ... | 1989 | 2911128 |
| yellow fever virus proteins ns2a, ns2b, and ns4b: identification and partial n-terminal amino acid sequence analysis. | a series of fusion proteins corresponding to the hydrophobic ns2 and ns4 regions of yellow fever virus (yf) were generated in escherichia coli using trpe fusion vectors. antisera to ns2 and ns4 region fusion proteins recognize virus-specific proteins of 15 and 27 kda, respectively. n-terminal amino acid sequence analysis of the 27-kda protein indicates that the n-terminus of yf ns4b immediately follows a signalase-like cleavage site. additional sequence data generated by microsequence analysis o ... | 1989 | 2922923 |
| update: aedes albopictus infestation--united states, mexico. | 1989 | 2499758 | |
| replication of mayaro virus in aedes albopictus cells: an electron microscopic study. | the replication of mayaro virus in aedes albopictus cells, was studied by electron microscopy at various times post-infection. in infected cells we observed the presence of cytoplasmic vesicles containing viral nucleocapsids and mature virus particles but at no time did we detect virus budding into such vacuoles. budding of virus through plasma membrane was rarely observed. our results are discussed considering the possibility of the release of virus particles to the extracellular space by exocy ... | 1989 | 2539797 |
| flaviviruses can mediate fusion from without in aedes albopictus mosquito cell cultures. | flavivirus-induced polykaryocytes were detected in monolayers of aedes albopictus (clone c6/36) mosquito cells as early as 20 min after adsorbing virus to these cells. a high multiplicity of infection with dengue (den)-1, 2, 3, 4, japanese encephalitis, and yellow fever viruses was required to demonstrate fusion from without (ffwo) with these flaviviruses. optimal conditions for ffwo included exposure of adsorbed virus to ph 6.0 and an incubation temperature of 39 degrees c. den-2 monoclonal ant ... | 1989 | 2543161 |
| maintaining mosquito cell lines at high temperatures: effects on the replication of flaviviruses. | the upper thermal limit for maintenance of eleven mosquito cell lines was studied. although most cell lines could be grown at 32 degrees c to 34 degrees c, anopheles stephensi cell line could be maintained at 37 degrees c. at higher temperatures initial growth rate was higher, but yield of cells after about a week of incubation was lower than at the standard temperature (28 degrees c). replication of several flaviviruses in aedes albopictus cell cultures adapted to 34.5 degrees c was faster, and ... | 1989 | 2563994 |
| purification and properties of a 28-kilodalton hemolytic and mosquitocidal protein toxin of bacillus thuringiensis subsp. darmstadiensis 73-e10-2. | the mosquitocidal crystal of bacillus thuringiensis subsp. darmstadiensis 73-e10-2 was purified, bioassayed against third-instar aedes aegypti larvae (50% lethal concentration, 7.5 micrograms/ml), and subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis, revealing polypeptides of 125, 50, 47, and 28 kilodaltons (kda). when solubilized and proteolytically activated by insect gut proteases or proteinase k, the crystal was cytotoxic to insect and mammalian cells in vitro and was h ... | 1989 | 2566594 |
| modulation in response to temperature of mayaro virus proteosynthesis in aedes albopictus cells. | incubation of aedes albopictus cells infected with mayaro virus at 37 degrees c causes inhibition of virus replication. during the first hour post infection (p.i.) incubation at 37 degrees c inhibited cellular and virus proteosynthesis. a preferential translation of heat shock proteins 82 kd and 70 kd was observed. after incubations longer than 1 hr at 37 degrees c, a switch to normal pattern of cell protein synthesis occurred without recovery of virus proteosynthesis. in addition, preferential ... | 1989 | 2570510 |
| clinical observations of mosquito bite reactions in man: a survey of the relationship between age and bite reaction. | to evaluate the mechanism of mosquito bite reaction in man, the reaction to aedes albopictus was observed in 162 subjects ranging in age between 1 to 68 years old. bite reactions were found to consist of both an immediate and a delayed reaction. the eruption and time course of the immediate reaction were consistent with type i hypersensitivity. the eruption and time course of the delayed reaction were consistent with cutaneous basophil hypersensitivity. positive rates of immediate reaction incre ... | 1989 | 2571626 |
| breeding habitats of mosquitoes in goa. | studies carried out in goa from 1986 to 1987 revealed immature stages of 43 species of mosquitoes. breeding habitats were divided into three categories, viz., (i) ground water habitats consisting of ground pools and tanks, rocky pools, paddy fields and stream beds, yielding 30 species; (ii) plant habitats consisting of tree holes and fallen coconut shells and leaf sheaths yielding 20 species; and (iii) domestic/peridomestic habitats consisting of cement tanks, glass, earthen, metallic containers ... | 1989 | 2572463 |
| relative suitability of aedes albopictus and aedes aegypti in north carolina to support development of dirofilaria immitis. | the relative suitability of two colonies established from local strains of aedes albopictus (wilmington and rockingham) and a local (raleigh) and laboratory (liverpool) strain of ae. aegypti to support development of dirofilaria immitis was investigated. high levels of mortality occurred 1-2 days after mosquitoes fed on a heavily microfilaremic (28,617 microfilariae/ml) dog but not when mosquitoes were fed on a dog exhibiting a moderate microfilaremia (3,300 microfilariae/ml). at 15-16 days post ... | 1989 | 2584971 |
| recently introduced aedes albopictus in the united states: potential vector of la crosse virus (bunyaviridae: california serogroup). | a population of aedes albopictus collected in 1986 in harris county, texas, was evaluated for its vector competence with 4 california serogroup viruses (jamestown canyon, keystone, la crosse and trivittatus). rates of midgut infection, dissemination of virus beyond the midgut and oral transmission to suckling mice were markedly different for the 4 viruses in a pattern representative of the antigenic relationships known for the california serogroup. only la crosse virus was shown to be efficientl ... | 1989 | 2584976 |
| records of aedes albopictus, ae. aegypti and ae. triseriatus from the u.s. air force ovitrapping program--1988. | 1989 | 2584979 | |
| size of blood meals of aedes albopictus and culex tritaeniorhynchus (diptera: culicidae) feeding on an unrestrained dog infected with dirofilaria immitis (spirurida: filariidae). | blood meal volume was determined for two species of mosquitoes, aedes albopictus (skuse) and culex tritaeniorhynchus giles, recently demonstrated to be natural vectors of canine heartworm in japan. when fed on unrestrained dogs, volumes were from 1 nl to 6 microliters in both species, but percentages of engorged mosquitoes were 45.2-64.3% for ae. albopictus and 11.9-22.2% for cx. tritaeniorhynchus. ae. albopictus preferred to feed on human bait versus canine bait when both were exposed concurren ... | 1989 | 2585448 |