Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| antinuclear antibodies in patients with atopic dermatitis and severe facial lesions. | in adult patients with atopic dermatitis (ad), the presence of autoantibodies such as anti-ige and antinuclear antibodies (ana) has been demonstrated. the patients may have altered immune regulation. | 1994 | 8003783 |
| [a new test of specific bronchial provocation with powder allergens in diagnosis of allergic diseases]. | the value of the melillo method of the specific bronchial provocation test (sbpt) using house dust mite allergen was examined. the studies were carried out on 33 mild asthma patients, found to be sensitive to house dust mite by the case history and positive skin test with dermatophagoides pteronyssinus. the lung function tests of most of the patients were within normal range. a positive bronchial response to allergen was observed in 26 persons (79% of patients examined), dual reaction in 16, onl ... | 1994 | 7951085 |
| effect of specific immunotherapy in dermatophagoides pteronyssinus allergic children. | the effect of immunotherapy on 21 dermatophagoides pteronyssinus (dpt)-sensitive patients (15 children with extrinsic asthma and six children with extrinsic asthma plus allergic rhinitis) aged 8.9 +/- 0.71 years was studied. their subjective and objective respiratory symptom score was recorded before beginning and after the first year of immunotherapy. the respiratory subjective symptoms, such as cough day/night, running nose, sputum production, sneezing, shortness of breath at night and asthma ... | 1994 | 8203258 |
| specific ige antibodies and serum eosinophil cationic protein in children with atopic dermatitis alone. | both eosinophils and specific immunoglobulin e (ige) to foods and mites have been considered involved in the pathogenesis of atopic dermatitis (ad). the relationship between eosinophils and specific ige, however, remains to be elucidated. blood eosinophil counts, serum eosinophil cationic protein (ecp) and ige to egg white, cow's milk, soybean, rice and dermatophagoides pteronyssinus (dp) were measured in subjects with ad alone or bronchial asthma (ba) alone. subjects with positive ige titers (p ... | 1994 | 8203257 |
| changes in nasal specific ige to mites after periods of allergen exposure-avoidance: a comparison with serum levels. | variations of serum and nasal specific ige to dermatophagoides pteronyssinus (der p) during alternate periods of antigen avoidance-exposure have been evaluated with an open design in a group of allergic children with asthma and rhinitis at the residential house istituto pio xii (misurina, bl, italy), at 1756 m, in the italian dolomites. a method based on direct incubation of allergen coupled substrate on the nasal mucosa has been employed to evaluate the levels of nasal ige. serum specific ige d ... | 1994 | 8039025 |
| sensitization to storage mites in house dust mite (dermatophagoides pteronyssinus) allergic patients. comparison of a rural and an urban population. | in this study, sera collected from 50 patients (24 females, 26 males) with type i allergy to house dust mite (dermatophagoides pteronyssinus) were investigated for ige antibodies specific for eight different mite species including storage mites of the families pyroglyphidae, glycyphagidae and acaridae. according to their environment the patients were divided into two groups. group i consisted of 24 (11 women, 13 men) farmers working and living in rural regions of austria (styria, lower austria), ... | 1994 | 8039020 |
| airways of allergic rhinitics are 'primed' to repeated allergen inhalation challenge. | the hypothesis that repeated exposure to a specific allergen will further increase bronchial responsiveness to that allergen is supported by indirect evidence. however, it has not been tested as intensely in the laboratory setting, and in some cases, conflicting results are presented. in order to test the hypothesis in the atopic subjects, allergen inhalation challenge tests were performed in 29 house dust mite (dermatophagoides pteronyssinus) sensitive subjects with allergic rhinitis. nine subj ... | 1994 | 8039019 |
| quantitative skin prick tests and specific ige (cap system) for d. pteronissynus--correlation of results in a paediatric population. | the cap system compared with rast has a greater sensitivity, with the same specificity, as well as an excellent correlation with skin prick tests (spt), as documented by others. standardization of spt is essential for routine and investigational purposes. we used a standardized lancet (dhs), and the methodology of reading the spt was a computerized digitalized graphics tablet coupled to an ibm-pc at using cad software, expressing the result as wheal areas with cut off +/- at 7 mm2. the method is ... | 1994 | 8185819 |
| serum levels of soluble il-2r, il-4, and soluble fc epsilon rii in adult bronchial asthma. | examination was made of the serum levels of interleukin 4 (il-4), soluble fc epsilon rii (sfc epsilon rii), and soluble interleukin 2 receptor (sil-2r) in 77 adult patients with bronchial asthma. all the patients had mild to moderate asthma and their symptoms were well controlled. the results were compared with values for 75 control subjects to clarify the involvement of these factors in the pathogenesis of bronchial asthma. serum sil-2r was elevated in asthmatics compared with control subjects ... | 1994 | 8131525 |
| cloning and sequencing of the dermatophagoides pteronyssinus group iii allergen, der p iii. | house dust mites are widely recognized as major factors involved in the triggering of allergic diseases such as asthma. it is now apparent that the group iii allergens of the dermatophagoides mite species may play a significant role in a number of house dust mite allergic cases. natural der p iii was isolated by gel filtration of salt precipitated dermatophagoides pteronyssinus extract and as reported previously ran as a doublet of mr 28 and 30 k on sodium dodecyl sulphate-polyacrylamide gel ele ... | 1994 | 8012853 |
| an immunogenetic analysis of t-cell reactive regions on the major allergen from the house dust mite, der p i, with recombinant truncated fragments. | proliferation assays were used to localize the t-cell reactive sites on the major allergen from the house dust mite dermatophagoides pteronyssinus, der p i. seven overlapping recombinant fragments of the der p i molecule, synthesized with the pgex expression vector system, were used to stimulate peripheral blood lymphocytes from 35 hdm-sensitive individuals. the fusion fragments were from 39 to 114 amino acids in length and spanned the entire der p i molecule. significant proliferative responses ... | 1994 | 7512103 |
| allergen skin test reactivity in an unselected danish population. the glostrup allergy study, denmark. | the aim of this study was to assess the distribution of allergen skin test reactivity in an unselected danish population. a total of 793 subjects, aged 15-69 years, were invited, and 599 (75.5%) attended. the skin prick test was performed with standardized allergen extracts of high potency. skin reactivity occurred in 28.4% of the subjects. the frequency of skin reactivity to the specific allergens ranged from 1.5% (cladosporium) to 12.5% (dermatophagoides pteronyssinus), and the frequencies of ... | 1994 | 8172364 |
| twenty-four-hour time course of eosinophil granule proteins ecp and epx during bronchial allergen challenges in serum of asthmatic children. | to study in vivo monitoring variables for bronchial allergen challenges, we investigated the time course of the eosinophil granule proteins, eosinophil cationic protein (ecp) and eosinophil protein x (epx) after allergen provocation in serum. thirty-two asthmatic children sensitive to house-dust mites and six healthy young adult controls were challenged by bronchial allergen provocations with dermatophagoides pteronyssinus and d. farinae. blood samples were taken at regular intervals up to 24 h. ... | 1994 | 8172362 |
| a three-year double-blind placebo-controlled study with specific oral immunotherapy to dermatophagoides: evidence of safety and efficacy in paediatric patients. | very few double-blind trials of oral immunotherapy have been reported. the majority of these have been performed with pollen extracts and the results have often been equivocal. the major weaknesses of these studies have been the short periods of the trials, the low doses of allergen employed and inadequate evaluation of efficacy. the present study has involved a placebo-controlled double-blind trial of oral immunotherapy for three years with dermatophagoides pteronyssinus at relatively high dose ... | 1994 | 8156447 |
| effect of nitrogen dioxide and sulphur dioxide on airway response of mild asthmatic patients to allergen inhalation. | air pollution may enhance the airway response of asthmatic subjects to allergen inhalation. to test the hypothesis that sulphur dioxide and nitrogen dioxide alone or in combination could have a contributory role, we have studied the effect of 6 h exposure to air, 200 parts per billion (ppb) sulphur dioxide, 400 ppb nitrogen dioxide, and the two gases together on the airway response to inhaled allergen in ten volunteers with mild atopic asthma. the subjects were exposed to the gases in random ord ... | 1994 | 7996960 |
| allergen-antibody complexes in the treatment of dermatophagoides pteronyssinus hypersensitivity diseases. | 1994 | 7889421 | |
| characterization of der p v allergen, cdna analysis, and ige-mediated reactivity to the recombinant protein. | a lambda gt11 library for cdna from dermatophagoides pteronyssinus was screened by plaque immunoassay, and a number of related clones that produced ige-binding proteins were isolated. their sequences were homologous to that of a cdna described previously, which contained a reading frame encoding a 17 kd polypeptide and which as determined by serologic analysis corresponded to a protein with relative molecular mass of 14 kd in mite extracts. the cdna from the lambda gt11 clones was truncated so i ... | 1994 | 7798547 |
| comparative detection of mite allergens in house dust of homes in moscow by enzyme-linked immunosorbent assay and acarologic analysis. | the present study revealed that 73% of surveyed apartments in moscow whose residents included children with the atopic form of bronchial asthma and sensitization to dermatophagoides pteronyssinus allergens were infested with the pyroglyphid mites d. pteronyssinus and d. farinae. the number of mites in the surveyed apartments varied between 0 and 154 mites/g of dust for d. pteronyssinus and between 0 and 162 mites/g of dust for d. farinae. the levels of mite allergens in these apartments ranged f ... | 1994 | 7709990 |
| comparison of the levels of the major allergens der p i and der p ii in standardized extracts of the house dust mite, dermatophagoides pteronyssinus. | allergy to the house dust mite dermatophagoides pteronyssinus is mediated by ige to the major allergens der p i and der p ii in the majority of mite-allergic patients. in recent years, standardized preparations of d. pteronyssinus, commercially available from several sources, have become widely used for the diagnosis and immunotherapy of mite allergy. as standardization implies uniformity of allergen composition and potency, we directly compared the absolute and relative quantities of der p i an ... | 1994 | 7874602 |
| allergens of the house dust mite, dermatophagoides pteronyssinus, in patients with mite allergic rhinitis: a clinical investigation by intracutaneous skin tests and nasal provocation tests. | to determine the allergens of mite allergic rhinitis, we studied 31 patients with mite allergic rhinitis by skin tests and nasal provocation tests (15 for skin and 16 for nasal tests) using 6 fractions of dermatophagoides pteronyssinus (dp) extract differing in molecular weights (15, 25, 32, 53, 95 and 190 kdmw). in skin testing, patients showed intense positive reactions to the fractions of 15, 25, 32, 95 and 190 kdmw, among which the most patients showed positive reactions to the fractions of ... | 1994 | 7863799 |
| filtration of airborne allergens in various households. | the filtration system investigated in this study uses the thermic convection caused by radiators for the adsorption of airborne particles. the effect of this filtration system on the reduction of clinical symptoms of allergic bronchial asthma was tested as well as its capability in retaining airborne allergens, in particular those of the house dust mite dermatophagoides pteronyssinus (d. pter.), which are responsible for this special kind of asthma. dust samples were taken before and after the h ... | 1994 | 7848501 |
| mite-antigen-stimulated cytokine production by peripheral blood mononuclear cells of atopic dermatitis patients with positive mite patch tests. | to investigate possible involvement of th1-type immunoreaction in the development of skin lesions of atopic dermatitis (ad), we measured mite-antigen-stimulated production of il-2, il-4 and ifn-gamma, using peripheral blood mononuclear cells (pbmc) obtained from 23 patients with ad who developed positive patch test reactions to house dust mite antigens extracted from dermatophagoides pteronyssinus (dp). incubation of these pbmc with the dp antigen for 72 h produced marked secretion of il-2 and i ... | 1994 | 7842675 |
| benzyl-benzoate foam: effects on mite allergens in mattress, serum and nasal secretory ige to dermatophagoides pteronyssinus, and bronchial hyperreactivity in children with allergic asthma. | home mattresses of 24 asthmatic children with house dust mite allergy were sprayed with either benzyl-benzoate foam or placebo in a double blind fashion, 10 days before the children left the residential house for asthmatic children istituto pio xii (located in the italian alps in an environment free of mites) and went back to their own home for the christmas and easter holidays. a further group of 8 children, whose mattresses received no treatment, was kept as an absolute control. two days after ... | 1994 | 7838620 |
| the profiles of interleukin (il)-2, il-6, and interferon-gamma production by peripheral blood mononuclear cells from house-dust-mite-allergic patients: a role for il-6 in allergic disease. | we have developed a model to measure cytokine production by peripheral blood mononuclear cells (pbmc) in vitro. in this report, we examine the production of interleukin-2 (il-2), il-6, and interferon-gamma (ifn-gamma) by pbmc of house-dust-mite (dermatophagoides pteronyssinus)-allergic subjects. when stimulated with specific allergen (d. pteronyssinus), pbmc of patients produced significant levels of il-2 and high levels of il-6, but little or no ifn-gamma. nonatopic control pbmc also produced i ... | 1994 | 7695065 |
| peak-flow variability in asthmatic children is not related to wall-to-wall carpeting on classroom floors. | the hypothesis was tested that wall-to-wall carpets on school classroom floors have a negative effect on the respiratory health of asthmatic children. asthmatic patients (n = 98) were selected from the records of the sophia children's hospital in rotterdam. the patients were between 5 and 11 years old. all children had peak-flow measurements three times a day for a 1-month period. the parents of the children completed a diary in which respiratory symptoms and medication use were recorded daily f ... | 1994 | 7695061 |
| specificity of elisa for igg subclass antibodies against inhalant antigens in early childhood. | elisa is increasingly used to measure antibodies in new circumstances. recently, it has been applied to the measurement of igg subclass antibodies against common antigens in early childhood. these studies have raised concerns about the specificity of some of these assays. this paper details the results of experiments which have assessed the specificity of igg1 binding to allergens of dust mite (dermatophagoides pteronyssinus) and ryegrass (lolium perenne) pollen by inhibition elisa in the sera o ... | 1994 | 7695060 |
| identification of a cross-reactive allergen (presumably tropomyosin) in shrimp, mite and insects. | a monoclonal antibody to dermatophagoides pteronyssinus is described that cross-reacts with an ige-binding antigen present in insects, crustacea (e.g. shrimp) and other invertebrates. by means of sodium dodecyl sulfate-polyacrylamide gel electrophoresis, gel filtration and immunofluorescence it was shown that this monoclonal antibody presumably recognizes tropomyosin. tropomyosin was shown to be involved in cross-reactivity between mite, shrimp and insects in shrimp-allergic patients. | 1994 | 8086829 |
| dermatophagoides pteronyssinus ii-induced interleukin-4 and interferon-gamma expression by freshly isolated lymphocytes of atopic individuals. | cytokines are known to play a major role in mediating many of the immunological and pathological features of allergic disease. much of our understanding of cytokine production in response to allergens has come from studying allergen-specific t cell clones following long-term in vitro culture. this has largely been due to the lack of sufficiently sensitive assays to measure allergen-induced cytokine production by freshly isolated peripheral blood mononuclear cells (pbmcs). here we have used the p ... | 1994 | 7812889 |
| evaluation of allergenic potency by reast inhibition. a new tool for the standardization of allergenic extracts. | the potency of allergenic extracts can be determined in vitro by rast inhibition, and this has become the preferred method for the standardization of allergens. a disadvantage of this technique is the impossibility of obtaining data about allergens bound to the solid phase, i.e., the counterpart of the inhibiting extract. the reast (reverse enzyme allergosorbent test) is based on the capture of ige by a specific antibody bound to microtiter wells, the reaction of captured ige with biotinylated a ... | 1994 | 8046253 |
| immune reactivity to der p i and der p ii in house dust mite sensitive patients attending paediatric and adult allergy clinics. | a comparison was made of immune responses to house dust mite allergens in symptomatic patients from paediatric and adult allergy clinics. ige-specific immune reactivity to dermatophagoides pteronyssinus was examined by western blotting and, for der p i and der p ii, by radioimmune dot-blot using purified allergens. nineteen mite-sensitive children (mean age 9 years) and 26 adults (mean age 31 years) were compared. positive ige responses by dot-blot were found to der p i and der p ii in 79% of ch ... | 1994 | 7982123 |
| a comparative study of three serine proteases from dermatophagoides pteronyssinus and d. farinae. | studies have shown that the dust mites dermatophagoides pteronyssinus and d. farinae contain several serine proteases, two of which have been shown to be allergenic, and to include trypsin and chymotrypsin, corresponding to the groups iii and vi mite allergens. however, mites also contain other serine proteases, and the data reported in this study show that an elastase-like enzyme is present in both species. this enzyme was differentiated from the other serine proteases, particularly chymotrypsi ... | 1994 | 7825723 |
| population analysis of cellular responses to synthetic peptides of der p ii, a major allergen molecule of dermatophagoides pteronyssinus, in allergic and nonallergic subjects. | responses of peripheral blood mononuclear cells to synthetic oligopeptides of der p ii, one of the major allergen molecules of dermatophagoides pteronyssinus, were compared between allergic and nonallergic subjects. healthy subjects showed positive responses to crude extracts of d. pteronyssinus, but only allergic subjects showed elevated cellular responses to der p ii. we synthesized three oligopeptides of der p ii in which motifs of a possible t-cell epitope were included. of 14 subjects with ... | 1994 | 7521142 |
| postallergen inhaled budesonide reduces late asthmatic response and inhibits the associated increase of airway responsiveness to methacholine in asthmatics. | to determine whether inhaled budesonide given after allergen inhalation challenge inhibits the late asthmatic response (lar) and/or the associated increase of airway responsiveness to methacholine, we performed a double-blind randomized cross-over study in 12 adult asthmatics (eight male, four female; mean age, 20.3 yr; range, 18 to 29 yr) sensitized to dermatophagoides pteronyssinus (dp) previously shown to develop early and late asthmatic response to allergen challenge with dp. on different da ... | 1994 | 8004297 |
| [current trends in the specific immunotherapy for allergic syndromes due to dermatophagoides pteronyssinus]. | house dust mites of the species dermatophagoides pteronyssinus are among the most prevalent perennial allergens. measurement of allergen exposure is so crucial to establish the natural history of the allergic reaction. the most common clinical manifestations induced by dermatophagoides are perennial asthma and perennial rhinitis. recently there are a number of studies indicating that a high degree of exposure to house dust mites may represent a risk factor for severe disease in patients with ato ... | 1994 | 8023008 |
| [the efficacy of long-term specific immunotherapy for dermatophagoides pteronyssinus in patients with atopic dermatitis]. | the aim of this study was to evaluate the efficacy of hyposensitization therapy with dermatophagoides pteronyssinus vaccine in patients with moderate and severe atopic dermatitis and sensitization to house mite dust. we studied 32 patients who showed strongly positive skin prick test and high levels of circulating specific ige to dermatophagoides pteronyssinus. all the patients were treated with sodium alginated dialyzed allergenic extract (bayropharm, milan), realized by increasing progressivel ... | 1994 | 8023005 |
| farm workers' occupational allergy to tetranychus urticae: clinical and immunologic aspects. | a group of 46 farm workers (32 men), affected by a recurrent "occupational disease of undetermined origin", underwent an immunologic investigation with a tetranychus urticae (tu) whole-body extract (tu-wbe) prepared in our laboratory. the patients suffered from seasonal attacks of rhinitis, during the summer and autumn periods, when working in open fields (30 subjects) or in greenhouse flower cultivation (16 subjects). in most patients, rhinitis was associated with bronchial asthma (16 subjects) ... | 1994 | 8074267 |
| allergen-induced cd23 on cd4+ t lymphocytes and cd21 on b lymphocytes in patients with allergic asthma: evidence and regulation. | interaction of cd4+ t cells and b cells is necessary for ige production. it has been recently demonstrated that cell surface antigen cd21 is a ligand for cd23 (fc epsilon rii) and that the pairing of these molecules may participate in the control of ige production. in this study we investigated the effect of the dermatophagoides pteronyssinus (dpt) allergen and recombinant interleukin(ril)-4 on the expression of cd21 and cd23 on t and b cells of asthmatic patients allergic to dpt and of healthy ... | 1994 | 7910139 |
| overlapping t-cell epitopes in the group i allergen of dermatophagoides species restricted by hla-dp and hla-dr class ii molecules. | the induction of ige antibodies reactive with the group i allergen of dermatophagoides species (house dust mite [hdm]), which comprise a major component of the allergic immune response in hdm-atopic individuals, is dependent on the functional activity of specific cd4+ t cells. in this report we demonstrate that for a particular hdm-atopic individual the t-cell response to the group i allergen of dermatophagoides pteronyssinus (der p i) is limited to a single region (residues 101-143) of the prot ... | 1994 | 7514196 |
| sensitization to blomia tropicalis: skin test and cross-reactivity studies. | this study evaluated the prevalence of positive percutaneous skin test results to blomia tropicalis, dermatophagoides pteronyssinus, and d. farinae in 167 consecutive persons (61 males, 106 females; mean age, 38.7 years; range, 14 to 75 years) with asthma or rhinitis, or both, in tampa, fla. forty-nine had asthma with or without rhinitis, and 118 had rhinitis alone. rast and rast inhibition analysis of all three mite species were performed by using pooled patient sera. prepared extracts of b. tr ... | 1994 | 8083450 |
| abnormal igg4 antibody response to aeroallergens in allergic patients. | various studies have suggested the involvement of immunoglobulin g4 (igg4) antibodies (ab) in the physiopathology of allergic disorders. recently, an abnormal igg4 ab production in response to immunization has been reported in some atopic patients. thus, in order to evidence in allergic patients, a potential abnormal igg4 ab response to aeroallergens following natural exposure, we compared, in 34 patients sensitive to dermatophagoides pteronyssinus and in 16 healthy subjects, the igg4 ab respons ... | 1994 | 8199463 |
| antigen presentation by human dermal microvascular endothelial cells. immunoregulatory effect of ifn-gamma and il-10. | human dermal microvascular endothelial cells (hdmec) play a central role in many aspects of the inflammatory and immune reactions in skin. hdmec display a phenotypic diversity ranging from cells with an epithelioid morphology to those that show both morphologic and biochemical characteristics of macrophages. here it is shown that hdmec possess the capability to both process and present ags. t lymphocyte clones specific for peptide p94-104, which are derived from the protein of group i allergen o ... | 1994 | 8207203 |
| evaluation of the overall degree of sensitization to airborne allergens by a single serologic test: implications for epidemiologic studies of allergy. | in most epidemiologic studies of respiratory allergy, the overall degree of sensitization is usually measured by indices based on skin prick test reactions to a panel of relevant airborne allergens. in the present study we used a single assay (cap-phadiatop, pharmacia, uppsala, sweden), which is based on the reaction between serum-specific ige and a mixture of locally relevant inhalant allergens to measure the degree of sensitization to inhalants in the sera of 1815 young italian men, in whom a ... | 1994 | 8308184 |
| immunobiology of the serine protease allergens from house dust mites. | extracts of dermatophagoides pteronyssinus and d. farinae were shown to contain a variety of 30 kda serine proteases, including trypsin, chymotrypsin, and an elastase-like enzyme. the mite trypsin, unlike chymotrypsin and the elastase enzyme, was heterogeneous with regard to charge. the enzymes were shown to be present at higher concentration in fecally enriched extracts than in whole mite extracts. the proteases were shown to induce vascular permeability and to detach cells in tissue culture. f ... | 1994 | 8116631 |
| house dust mite allergen (der p i) and respiratory symptoms in children: a case-control study. | the association between house dust mite allergen in house dust and childhood respiratory symptoms was investigated in a case-control study of 259 children with reported chronic respiratory symptoms and 257 control children without reported respiratory symptoms. the der p i concentration in floor dust of the living room and bedroom and in mattress dust was determined using an enzyme immunoassay. venous blood samples were taken from all children for serum ige determination against house dust mite ... | 1994 | 7874605 |
| mite antigen in house dust: relationship with different housing characteristics in the netherlands. | as part of a case-controlled study on the relationship between home dampness and respiratory symptoms of children, the concentration of the major allergen of dermatophagoides pteronyssinus (der p i) in floor dust and mattress dust in 516 dwellings in the netherlands was measured. a checklist, completed by the investigators, was used to obtain information on home and occupant characteristics, which may have an impact on the der p i concentration in house dust. the geometric mean mite antigen conc ... | 1994 | 7812886 |
| comparison of two epidemiological protocols for measuring airway responsiveness and allergic sensitivity in adults. | in recent years, airway responsiveness has commonly been measured in epidemiological studies using one of two methods. in one method, histamine is administered via a handheld devilbiss nebulizer and in the other, methacholine is administered via a dosimeter. allergic sensitivity has commonly been measured by either the allergen droplet method or by phazet. we wanted to assess the comparability of airway responsiveness and of allergic sensitivity measured by both methods. a total of 48 volunteers ... | 1994 | 7828688 |
| atopic sensitization in children of somali immigrants in italy. | children of immigrants are known to be at greater risk for developing allergic manifestations than the population in general. in this study we observed children of somali origin living in italy in order to find plausible explanations for the high risk of allergic disease in immigrants. fifty-two children aged 0-14 years (mean = 6.7 years) were examined during the spring season. the mothers of the children were asked to fill in a questionnaire on symptoms and signs of atopic diseases and the fami ... | 1994 | 7850033 |
| cow milk allergy within the spectrum of atopic disorders. | in order to examine the relationship between cow milk allergy (cma) and atopic disorders in childhood, a consecutive group of 42 infants with ige mediated cma was followed for at least 2 years. the incidence of sensitization to common food and inhalant antigens and the development of eczema, asthma, and food allergies was examined for the cohort and compared between patients whose cma remitted and those with persistent disease. in this cohort the prevalence of eczema was 57%, asthma 69%, egg all ... | 1994 | 7889427 |
| genetic linkage of t-cell receptor alpha/delta complex to specific ige responses. | ige responses to inhaled proteins underlie the clinical syndrome of allergic (atopic) asthma and rhinitis. we have investigated genetic linkage between specific ige reactions to highly purified major allergens and the t-cell receptor (tcr) alpha and beta gene complexes on chromosome 14 and 7, respectively. antigens tested included highly purified proteins from the housedust mite dermatophagoides pteronyssinus, the domestic cat and dog, grass pollen, and the mould alternaria alternata. affected s ... | 1994 | 7911920 |
| evaluation of the potency of allergenic extracts by inhibition of igg-antibody binding. | a method is described for the in vitro potency evaluation of allergenic extracts by using their capacity of binding to specific human igg antibodies. the results obtained with this inhibition-type enzyme immunoassay are compared with the analyses by the customary method of ige antibody binding. a large series of allergenic protein fractions from the pollen of the gramineae, olea europea, parietaria judaica, and from the dust mite dermatophagoides pteronyssinus as well as from cat dander and pean ... | 1994 | 7917232 |
| cloning and characterization of a major allergen of the house dust mite, dermatophagoides pteronyssinus, homologous with glutathione s-transferase. | a major allergen of the house dust mite, dermatophagoides pteronyssinus, has been identified and characterized from a lambda gt11 cdna library of the mite. ige antibodies from the sera of allergic patients that recognise the cloned polypeptide bind to an approximately 26 kda polypeptide on a western blot of reduced mite polypeptides. nucleotides sequencing of the clone revealed a 219 amino acid open reading frame encoding a protein with a derived molecular mass of 25,589 da and a pi of 6.3. comp ... | 1994 | 7918651 |
| human ige in scid mice reconstituted with peripheral blood mononuclear cells from dermatophagoides pteronyssinus-sensitive patients. | scid mice were reconstituted with purified peripheral blood mononuclear cells from patients sensitive to dermatophagoides pteronyssinus (dpt). after immunization with a low dose of the related allergen, these human (hu)-scid mice may develop a specific ige response. by using an ige-dependent platelet assay and the related allergen dpt, the human ige was demonstrated to be functional. indeed a high correlation was obtained between both parameters. no rise in human ige level was detected within 3 ... | 1994 | 7930597 |
| dietary sodium intake and the risk of airway hyperreactivity in a random adult population. | high dietary sodium intake has been identified as a potential cause of asthma and airway hyperreactivity. this study was designed to test the hypothesis that dietary sodium intake is an independent determinant of the risk of hyperreactivity in the general population, and to assess the role of atopy in the association between these factors. | 1994 | 7940426 |
| comparison of three in vitro assays for serum ige with skin testing in asthmatic children. | the diagnostic performance of three commercial assay kits [phadezym rast (phrast), pharmacia cap system (cap), and multiple chemiluminescent assay (cla-mast)] for measuring serum-specific ige was evaluated and compared using intradermal skin testing or skin prick testing as reference standards. serum samples were obtained from allergic patients who were tested with either intradermal skin tests or skin prick tests (96 and 49 subjects, respectively). six different allergen extracts were tested: d ... | 1994 | 7944001 |
| [breeding control and immobilizing effects of wood microingredients on house dust mites]. | the possible effects of essential oils as wood microingredients on house dust mites (tyrophagus putrescentiae, dermatophagoides farinae and dermatophagoides pteronyssinus) were investigated. 1. whether small pieces of 5 types of wood had any control effect on mite breeding was studied. mite breeding using only normal feed was compared with breeding using feed mixed with small pieces of wood. in addition, mite breeding using feed mixed with small pieces of wood with no essential oil was studied. ... | 1994 | 7949285 |
| effect of regular terbutaline and budesonide on bronchial reactivity to allergen challenge. | there is a transient rebound increase in bronchial reactivity to histamine and methacholine following regular treatment with an inhaled beta 2-agonist. we set out to determine whether the response to allergen was increased after cessation of regular inhaled terbutaline and whether concomitant inhaled budesonide modifies this response. in a double-blind, double-dummy, parallel group design we studied 41 subjects (37 evaluable) with mild asthma who were allergic to dermatophagoides pteronyssinus, ... | 1994 | 7952551 |
| mite allergen exposure is a risk for the incidence of specific sensitization. | mite allergen exposure is essential for the process of specific allergic sensitization. however, it is not clear whether incidence of specific sensitization at school age depends on the level of mite antigen exposure. therefore the relationship between mite allergen exposure and incidence of specific sensitization was investigated. | 1994 | 8027498 |
| do latex allergens potentiate other skin tests? | we report the case of a 33 year old atopic female patient, who worked for the last 4 years as a cleaner in the surgical area of a hospital. one year after starting the work in this area, the patient noticed upper respiratory symptoms and soon later dyspnea immediately after entering the operating room. other allergic symptoms were perennial rhinoconjunctivitis and urticaria after contact with peaches. common skin prick tests were positive for dermatophagoides pteronyssinus. fifteen min later a s ... | 1994 | 8030583 |
| asthma, allergy and atopy in southern chinese school students. | in order to determine the prevalence of asthma, allergy and atopy in southern china and the relative importance of atopy and parental history in predicting asthma and allergic diseases, we carried out a cross-sectional study on 737 secondary school students aged 12-20 (492 boys, 245 girls; mean age = 16.4 years, sd = 1.8 years) in the city of san bu, guangdong, china. standard questionnaires on respiratory and allergic symptoms were distributed for completion by parents with an overall response ... | 1994 | 8039021 |
| ige to food allergens are highly prevalent in patients allergic to pollens, with and without symptoms of food allergy. | serum samples from 274 patients allergic to one or more of three pollens (birch, grass, mugwort), from 36 patients allergic to cat and/or dermatophagoides pteronyssinus but not to pollen and from 55 non-allergic controls, as well as 20 cord blood samples, were examined for specific ige to six 'pollen-associated' food allergens by using a new sensitive assay (cap). a questionnaire asking for reactions to food was also sent to all patients. in the pollen group, 111 patients (47%) were positive (> ... | 1994 | 8039023 |
| isolation of three species of mites from house dust of atopic dermatitis patients in qualyobia governorate, egypt. | dermatitis or inflammation of the skin caused by an outside agent, is a condition with many causes. it may result from direct irritation of the skin by a substances (chemical or insecticide) or it may be an allergic reaction to a particular substance that has been in contact with the skin as soap or detergent or insect urticating hairs; injected as insect saliva or faeces or sting or taken by mouth as food or drug. in general, treatment of dermatitis depends mainly upon the cause. in the present ... | 1994 | 8077751 |
| interleukin-1 favours the in vitro development of type 2 t helper (th2) human t-cell clones. | the effects exerted by interleukin-1 (il1) on the growth and differentiation of human th1 and th2 cells were examined. neither il1 nor the il1 receptor antagonist (il1ra) had detectable activity toward the antigen- or anti-cd3 antibody-induced proliferative response of already established type 1 t helper (th1) or type 2 t helper (th2) clones. moreover, neither exogenous il1 addiction to, nor neutralization of, endogenously produced il1 in bulk cultures before cloning changed the th1-like cytokin ... | 1994 | 8079050 |
| quantitative analysis of peripheral allergen-specific b lymphocytes in asthma. | we have recently shown that the number of peripheral allergen-specific t cells can, in part, predict, together with methacholine hyperresponsiveness, the bronchial response to inhaled allergen in asthmatic patients. this study was designed to explore the role of blood b cells committed to produce allergen-specific ige in asthma. twenty-three asthmatic patients sensitized to dermatophagoides pteronyssinus and 11 control subjects were studied. peripheral blood b cells, committed to produce allerge ... | 1994 | 8092432 |
| asthma in gaza refugee camp children and its relationship with house dust mites. | three hundred asthmatic children aged 3 to 15 years, and 100 age-sex-neighborhood matched controls, were studied for environmental risk factors for asthma during 1986 to 1987. in 98% of the asthmatic patients, exposure to house dust aggravated the symptoms. in 97% of the cases, the symptoms were more severe at home; in more than 50%, the symptoms persisted throughout the year. significantly more cigarettes were smoked in the homes of the allergic children than in the control homes. house dust sa ... | 1994 | 8109806 |
| sensitization to inhalant allergens in wheezing infants is predictive of the development of infantile asthma. | early recognition of infantile asthma in wheezing infants is a major problem for physicians. we investigated whether detection of early sensitization to inhalant allergens would be useful to identify those wheezing infants who are likely to develop asthma. a total of 67 infants (aged 1-25 months) hospitalized for a wheezing episode were initially tested for reactivity to inhalant allergens by both skin prick test and in vitro measurement of specific ige antibodies (phadiatop). thirty-seven of th ... | 1994 | 7709993 |
| detection of at least one high-molecular-mass, ige-binding component of the dust mite lepidoglyphus destructor. | we studied the allergen composition in an extract of the storage mite lepidoglyphus destructor. monoclonal antibodies (mabs) were raised against l. destructor by a standard hybridoma technique. in the subsequent screening, we concentrated on mabs fulfilling three criteria. first, in elisa, mabs were assessed against a panel of various mite species, and only those reacting exclusively with l. destructor extract were selected for further analyses. secondly, mabs were selected in immunoblotting acc ... | 1994 | 7653740 |
| optimization and characterization of freeze-dried multilamellar liposomes incorporating different standardized allergen extracts. | desensitization therapy for type i allergy is now current practice. liposomes have been proposed as a support for allergens to improve safety and effectiveness. the aim of this work was to optimize liposomal formulations of three different standardized allergen extracts and to test their allergenicity in vitro and in a preclinical trial. allergen extracts (dactylis glomerata, dermatophagoides pteronyssinus, and cat hair and dander) were associated with multilamellar liposomes of varying composit ... | 1994 | 7653744 |
| il-10 secretion of allergen-specific skin-derived t cells correlates positively with that of the th2 cytokines il-4 and il-5. | in atopic individuals, allergen-specific cd4+ t lymphocytes often belong to the t-helper 2 (th2) subset as they secrete the marker cytokines interleukin-4 (il-4) and il-5 but not interferon-gamma (inf-gamma). il-10 is a cytokine the production of which, in the mouse system has been described to be restricted to the th2 subset, but in the human was found to be produced by both th1 and th2 t cell clones (tcc). we have recently shown that house dust mite antigen (dermatophagoides pteronyssinus)-spe ... | 1994 | 7749574 |
| skin disease-related t cells bind to endothelial selectins: expression of cutaneous lymphocyte antigen (cla) predicts e-selectin but not p-selectin binding. | cutaneous lymphocyte antigen (cla), defined by the heca-452 antibody, is a cell surface glycoprotein found on a subset of t cells in peripheral blood that binds specifically to e-selectin. this marker is present on the majority of t cells at sites of cutaneous inflammation and immune responses. based upon such evidence, an association between t cell cla expression and skin homing has been proposed. to understand better this relationship, we asked whether putative disease-related, antigen-specifi ... | 1994 | 7517361 |
| [histamine liberation and specific ige against dermatophagoides pteronyssinus in parasitized patients]. | we studied 98 patients with different parasitosis, without clinical symptoms of mite sensitization, most of them coming from guinea. histamine release to dermatophagoides pteronyssinus was performed using whole blood. specific ige to the same antigen was measured by east, as well as by an immunodot with the same antigen extract employed for the histamine release test. finally, the east positive sera were studied by immunoblotting. the presence of specific ige by east could be proved in 31 patien ... | 1994 | 7520207 |
| in vivo biological activity of recombinant der p ii allergen of house-dust mite. | the cdna coding for one of the major allergens of dermatophagoides pteronyssinus, der p ii, has been cloned and expressed in escherichia coli as a fusion protein with glutathione s-transferase. the recombinant material (r der p ii) was tested in the skin of house-dust allergic patients, and was found to be almost as active as the purified native allergen (n der p ii) in stimulating immediate hypersensitivity reactions. the positivity to r der p ii in these patients, who were selected for their s ... | 1994 | 7522071 |
| peptide specificity and hla restriction do not dictate lymphokine production by allergen-specific t-lymphocyte clones. | human and murine cd4+ t lymphocytes can be subdivided into distinct subsets [t-helper type 0 (th0), th1 or th2], based on their lymphokine production profiles. not much is known about the factors that determine these restricted lymphokine secretion profiles. peptide specificity and human leucocyte antigen (hla) restriction may be such factors. as it is well established that allergen-specific t lymphocytes from atopic individuals and non-atopic controls differ in their lymphokine secretion profil ... | 1994 | 7525459 |
| discrepancies between in vitro and in vivo tests for house dust mite allergy: in domestic exposure a better predictor than sensitization? | we subjected seven asthmatic children to two bronchial allergen challenges, first with an extract from the house dust mite dermatophagoides pteronyssinus (der p) and then dermatophagoides farinae (der f), or vice versa. all children had elevated specific serum ige to both species as well as reactions by crossed radioimmuno/electrophoresis (crie) to both group i and ii allergens from both species. immunoabsorption and subsequent analysis by crie showed a considerable concentration of serum ige wi ... | 1994 | 7531110 |
| allergenic characterization of acarus siro and tyrophagus putrescentiae and their crossreactivity with lepidoglyphus destructor and dermatophagoides pteronyssinus. | allergenic components in extracts of acarus siro and tyrophagus putrescentiae were identified by sodium dodecyl sulphate-polyacrylamide (sds-page) and immunoblotting. five and four allergenic components, respectively, were visualized with sera from farmers sensitized to storage mites. the highest frequency of ige-binding was to a 15 kda component of a. siro (7/9 sera) and a 16 kda component of t. putrescentiae (23/29 sera). the allergenic crossreactivity of a. siro and t. putrescentiae with lepi ... | 1994 | 7982124 |
| comparison of skin-prick test assay and reverse enzyme immunoassay competition (reina-c) for biological activity of allergens. | an in vitro technique for measuring the relative potency of allergenic extracts has been developed and compared with the dose-response skin-prick test. the in vitro technique is a modification of an elisa based on the method of antigen capture using polystyrene microtitre wells as solid phase (reina-competition) and is also based on a dose-response. both methods have been applied to allergen extracts of dermatophagoides pteronyssinus, phleum pratense, secale cereale, lolium perenne, plantago lan ... | 1994 | 8187028 |
| selective restimulation of antigen or allergen preactivated t cells using okt3 f(ab)2 results in the secretion of th-1 or th-2-like cytokine patterns. | the synthesis of ige is regulated by cytokines secreted from t-helper cells. the studies on cytokine secretion by peripheral blood mononuclear cells (pbmc) upon stimulation with antigen or allergen are difficult due to low levels of cytokines, especially of interleukin-4 (il-4). | 1995 | 8581844 |
| mechanism of allergic cross-reactions. v. high incidence of unanticipated cross-stimulation by natural allergens of rat basophilic leukemia cells sensitized with monoclonal ige antibodies. | the incidence of cross-stimulations by natural allergens was investigated using rbl-2h3 cells sensitized with five different mouse monoclonal anti-dnp iges and four mercury-induced rat monoclonal iges. cells sensitized with 3 of the 5 monoclonal anti-dnp iges (clones spe-7, srt-1, lb4) responded by serotonin release upon stimulation by natural allergens such as dermatophagoides pteronyssinus, horse dander and mugwort extracts. serotonin release could be inhibited by monovalent dnp-lysine, indica ... | 1995 | 7549509 |
| effect of diuretics on allergen-induced contractions of passively sensitized human bronchi in vitro. | inhaled furosemide has been shown to protect subjects with asthma from bronchoconstriction induced by a wide variety of stimuli, including allergen, but the mechanism of action is controversial. we have used an in vitro model of allergen-induced bronchoconstriction to examine the effects of furosemide and other ion transport inhibitors. human bronchial rings were passively sensitized by incubation with serum from an atopic donor and were challenged with dermatophagoides pteronyssinus. allergen-c ... | 1995 | 7551365 |
| [skin tests for different species of acari in asthmatic children from mexico city]. | house dust mites, dermatophagoides pteronyssinus (dp) and dermatophagoides farinae (df) are the predominant cause of allergic asthma in our population. to detect the presence of sensitivity to other mites besides dp and df in 100 children that live in mexico city, skin tests were performed in their arms with multi-test devices and glycerinated extracts. there were 34 female and 66 male, aged 4 to 14 years (x = 9.1y), with history of mild and moderate asthma, 2 months to 12 years of evolution. dp ... | 1995 | 7552183 |
| [frequency of allergens in immediate, late, and dual allergic skin response at the national institute of pediatrics]. | we studied 126 children with allergic illness in instituto nacional de pediatria. we applied cutaneous test to determine the early, late and dual response. the results were 68% for the early response, 6% late response and 26% dual response. the allergens more frequently found in the early and late response were dermatophagoides pteronyssinus, dermatophagoides farinae and house dust. approximately the third part of the children studied presented late cutaneous response (dual response more isolate ... | 1995 | 7552188 |
| [prevalence of sensitization to blomia in gran canaria]. | in the present study we assessed the prevalence of sensitizations to blomia in our environment. perennial climatological conditions in the canarian islands facilitates the habitat to mites. the blomia is found in tropical and subtropical homes of europe, usa and asia like the dermatophagoides. we have designed a transversal and prospective study on 100 patients who consulted our unit for the first time. a prick-test for the common allergens, including blomia kulagini, and in vitro determination ... | 1995 | 7572538 |
| quantitation of major allergens in dust samples from urban populations collected in different seasons in two climatic areas of the basque region (spain). | we present the results of allergen content evaluation in 80 dust samples from 31 homes of atopic patients from two climatic areas (humid and subhumid), collected in two seasons of the year (autumn and winter). monoclonal antibody-based immunoassays were used to quantify der p 1, der f 1, der 2, lep d 1, and fel d 1. the results were compared according to climate, season, and the type of sensitization (pyroglyphidae mites, storage mites, or grass pollens). we underline the predominance of dermato ... | 1995 | 7573840 |
| polyinosinic acid: polycytidylic acid promotes t helper type 1-specific immune responses by stimulating macrophage production of interferon-alpha and interleukin-12. | the effects exerted on the development in vitro of dermatophagoides pteronyssinus group i (der p i)-specific t cell lines and t cell clones by addition of polyinosinic acid: polycytidylic acid (poly i:c) in lymphocyte bulk culture were examined. der p i-specific t cell lines generated in presence of poly i:c exhibited reduced ability to produce interleukin (il)-4 and il-5 and developed into der p i-specific cd4+ t cell clones showing a t helper (th) type 0 or th1, instead of th0/th2 cytokine pro ... | 1995 | 7589141 |
| peptide-induced anergy in allergen-specific human th2 cells results in lack of cytokine production and b cell help for ige synthesis. reversal by il-2, not by il-4 or il-13. | the induction of anergy in t cells is believed to be the result of triggering of the tcr in the absence of adequate costimulation mediated through the interaction of cd28 and its ligands, cd80 and cd86. here, we demonstrate that stimulation of human group i allergen in dermatophagoides pteronyssinus extract (der p 1)-specific cd4+ th2-like t cell clones with der p 1-derived peptides in the absence of professional apc results in a state of nonresponsiveness. the induction of anergy occurred despi ... | 1995 | 7594575 |
| a major house dust mite allergen disrupts the immunoglobulin e network by selectively cleaving cd23: innate protection by antiproteases. | asthma is a chronic life-threatening disease of worldwide importance. although allergic asthma and related atopic conditions correlate strongly with immune sensitization to house dust mites, it is unclear why antigens from mites provoke such powerful allergic immune responses. we have characterized the protease activity of der p i, the group i protease allergen of the house dust mite dermatophagoides pteronyssinus, and here report that it cleaves the low-affinity immunoglobulin (ig) e fc recepto ... | 1995 | 7595223 |
| lack of association between hla-dq and -dr genotypes and asthma in southern chinese patients. | asthma is an inflammatory disease with a strong genetic predisposition. we have studied a group of unrelated asthmatic patients of southern chinese origin on their hla-dr and -dq regions using molecular techniques and compared them with 104 healthy controls of the same ethnic origin. restriction fragment length polymorphism (rflp) was used to genotype the mhc class ii dr beta, dq alpha and dq beta loci of the subjects. polymerase chain reaction (pcr) using sequence specific primer (ssp) for dq b ... | 1995 | 7600378 |
| four species of house dust mites recovered from houses of patients with allergic respiratory diseases. | arthropods and their products are wide spread and considered one of the common allergens affecting man. allergy to arthropod antigens is the major cause of respiratory allergy as rhinitis and bronchial asthma. the range of reaction depends on several factors which include the type and source of the material, the rate of intensity, the duration of exposure, the human immune response and history of prior exposure. successful management of allergic disease depends on proper identification of the ca ... | 1995 | 7602163 |
| human ige in severe combined immunodeficiency mice reconstituted with peripheral blood mononuclear cells from dermatophagoides pteronyssinus-sensitive patients. | we studied conditions of human ige formation in severe combined immunodeficiency (scid) mice engrafted with peripheral blood mononuclear cells (pbmcs) from allergic patients sensitive to dermatophagoides pteronyssinus (d.pt.). with 10 x 10(6) pbmcs injected intraperitoneally, the hu-scid mice developed an ige response, but only if experimental animals were immunized with the related allergen. two routes of immunization were tested: intraperitoneal and inhalation. in these experimental conditions ... | 1995 | 7613137 |
| immunochemical characterization of recombinant and native tropomyosins as a new allergen from the house dust mite, dermatophagoides farinae. | two predominant mite species, dermatophagoides farinae and dermatophagoides pteronyssinus, are responsible for the immediate hypersensitivity reaction. although a large number of antigens and allergens was detected in a whole mite culture extract, only the major allergens of der i and ii groups have been extensively studied. much information on other important allergens remains to be accumulated. | 1995 | 7622766 |
| food-induced and occupational asthma due to barley flour. | occupational exposure to inhalant allergens may induce asthma but the presence of asthma after the ingestion of the allergen is rarely reported. | 1995 | 7648375 |
| house dust mite fauna in thailand. | house dust mites have been shown to be the most important source of allergen in thailand. the results of our initial mite survey were in conflict with previous data reported 20 years ago. to evaluate the current condition of mite fauna in thailand, we conducted a larger scale survey of house dust mites in dusts from three different parts of the country. a total of 630 mattress dust samples was collected from various types of housing from central, northern and northeastern parts of thailand. hous ... | 1995 | 7648463 |
| bioassay techniques for evaluating pesticides against dermatophagoides spp. (acari: pyroglyphidae). | three bioassay techniques for testing pesticides against the american house dust mite, dermatophagoides farinae hughes, and the european house dust mite, dermatophagoides pteronyssinus (trouessart), were evaluated using cypermethrin as a standard. all 3 bioassay techniques gave linear responses, and can be used for evaluation of different properties of compounds. the slide dip technique tests for contact activity, the plastic petri dish technique for residual activity on a nonporous surface, and ... | 1995 | 7650713 |
| natural history of ige antibodies in children at risk for atopy. | the relationship between atopic diseases and high serum ige levels has been emphasized since the mid-sixties, when ige antibodies were discovered. a number of environmental factors, such as the type of feeding, passive smoking, viral infections, early mold, house dust mite and pet fur exposure modulate ige synthesis and affect the development of atopic diseases. | 1995 | 7749975 |
| house dust mite (dermatophagoides farinae and dermatophagoides pteronyssinus) prevalence in the rooms and hallways of a tertiary care hospital. | house dust mites, dermatophagoides farinae and dermatophagoides pteronyssinus, are important components in the development of asthma. | 1995 | 7722158 |
| dietary antioxidant vitamin intake and lung function in the general population. | we have investigated the relation between lung function and dietary intake of the antioxidant vitamins c and e in the general population in a cross-sectional survey of a random sample of adults from the electoral register of an administrative area of nottingham. in 2,633 subjects 18 to 70 yr of age, we measured fev1 and fvc, allergen skin sensitivity to grass pollen, cat fur, and dermatophagoides pteronyssinus, pack-years smoking exposure by personal recall, and usual dietary intake of vitamins ... | 1995 | 7735589 |
| canine atopic disease: the prevalence of positive intradermal skin tests at two sites in the north and south of great britain. | results of intradermal skin test responses to the same panel of 53 allergens were compared in 118 dogs with atopic disease presented at two geographical centres, edinburgh (87 cases) and london (31 cases). the allergens most commonly positive at both centres were human dander and dermatophagoides farinae, but positive tests to all of the allergens used occurred in at least one case. the mean number of allergens to which positive tests resulted in atopic dogs was 5.126 (edinburgh) and 5.129 (lond ... | 1995 | 7747407 |
| augmentation of permeability in the bronchial epithelium by the house dust mite allergen der p1. | sequence analyses have revealed the existence of homology between certain aeroallergens and proteolytic enzymes. this homology can be expressed functionally, but its significance to airway pathophysiology is unknown. studies with madin-darby canine kidney cells and canine tracheal epithelial cells grown on plastic substrata or matrix proteins suggest that der p1, a major allergen from the house dust mite dermatophagoides pteronyssinus and a cysteine proteinase, or the unfractionated growth mediu ... | 1995 | 7695916 |
| der p i, a major allergen of the house dust mite, proteolytically cleaves the low-affinity receptor for human ige (cd23). | the nature of the proteases that cleave cd23 in vivo is of considerable interest, but remains unknown. here, we demonstrate that der p i, a major allergen of the house dust mite dermatophagoides pteronyssinus, cleaves cd23 from the surface of cultured human b cells (rpmi 8866 b cell line). the cleavage of the receptor from the b cell surface was associated with a parallel increase in soluble cd23 (scd23) in the culture supernatant. furthermore, the proteolytic effect of der p i was specific for ... | 1995 | 7489763 |
| an immunogenetic analysis of the t-cell recognition of the major house dust mite allergen der p 2: identification of high- and low-responder hla-dq alleles and localization of t-cell epitopes. | cellular reactivity to der p 2, a major allergen of the house dust mite (hdm) dermatophagoides pteronyssinus, was studied in a group of 41 symptomatic hdm sensitive patients, using fresh peripheral blood mononuclear cells (pbmc) and assays of proliferation. sixty per cent of the patients responded to der p2, with reactivities being greater in patients with asthma as one of their clinical manifestations and also in those who had skin-test reactivity to a number of allergens. hla-dr and -dq seroty ... | 1995 | 7490115 |
| [prevalence of immediate allergy to respiratory allergens in fodder farmers in doubs]. | the doubs is a damp, semi-mountainous fodder farming department in which occupational respiratory diseases (including asthma) are common in farmers. we studied the prevalence of ige-mediated allergy (total ige, phadiatop and skin prick tests) in a group of 265 exclusive dairy farmers of both sexes of the department and in a control group of non exposed, administrative workers living in the same area. skin prick tests were: dermatophagoides pteronyssinus, acarus siro, cat hair, cow danders, grass ... | 1995 | 7501894 |