Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
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| a cross-sectional study on the potential transmission of the carcinogenic liver fluke opisthorchis viverrini and other fishborne zoonotic trematodes by aquaculture fish. | throughout southeast asia and china, eating raw and or partially cooked cyprinid fish causes liver (hepatobiliary) disease and cancer (cholangiocarcinoma) due to fishborne zoonotic trematodes (fzt), in particular clonorchis sinensis and opisthorchis viverrini. the primary source of transmission is by native fish, but aquaculture fish are also reported to have high infective potential. here, a cross-sectional survey of fzt in fish farms was conducted in an endemic area in khon kaen province, thai ... | 2013 | 23320421 |
| centrocestus formosanus (heterophyidae): human infections and the infection source in lao pdr. | in lao people's democratic republic (pdr), we detected 7 patients infected with centrocestus formosanus (1-122 adult specimens) after praziquantel treatment and purgation, together with several other trematode species including opisthorchis viverrini and haplorchis taichui. the patients were all men, 23-42 yr-of-age. three subjects were from vientiane municipality and 1 each were from khammouane, saravane, champassak, and xiengkhouang province. the patients had frequently eaten raw freshwater fi ... | 2013 | 23116489 |
| schistosome and liver fluke derived catechol-estrogens and helminth associated cancers. | infection with helminth parasites remains a persistent public health problem in developing countries. three of these pathogens, the liver flukes clonorchis sinensis, opisthorchis viverrini and the blood fluke schistosoma haematobium, are of particular concern due to their classification as group 1 carcinogens: infection with these worms is carcinogenic. using liquid chromatography-mass spectrometry (lc-ms/ms) approaches, we identified steroid hormone like (e.g., oxysterol-like, catechol estrogen ... | 2014 | 25566326 |
| [the reagents kit to detect metyorchis biis, opisthorchis viverrini and clonorchis sinensis, opisthorchis felineus--agents of opisthorchiasis using technique of polymerase chain reaction in real-time]. | the helminths opisthorchis felineus, opisthorchis viverrini, clonorchis sinensis, metorchis bilis are the agents of opisthorchiasis. the actual diagnostic of parasitic diseases based on microscope analysis of samples of human feces to detect presence of ova of parasites suffers of many shortcomings, in particular low sensitivity especially at earlier stages. the purpose of this study was to compare results of detection of parasites using both classical technique and technique of specific differe ... | 2014 | 25552048 |
| infection and cancer: global distribution and burden of diseases. | infection is one of the main risk factors for cancer. | 2014 | 25512154 |
| rapid detection and differentiation of clonorchis sinensis and opisthorchis viverrini using real-time pcr and high resolution melting analysis. | clonorchis sinensis and opisthorchis viverrini are both important fish-borne pathogens, causing serious public health problem in asia. the present study developed an assay integrating real-time pcr and high resolution melting (hrm) analysis for the specific detection and rapid identification of c. sinensis and o. viverrini. primers targeting cox1 gene were highly specific for these liver flukes, as evidenced by the negative amplification of closely related trematodes. assays using genomic dna ex ... | 2014 | 25386621 |
| zoonotic trematode metacercariae in fish from phnom penh and pursat, cambodia. | a survey was performed to investigate the infection status of freshwater fish with zoonotic trematode metacercariae in phnom penh and pursat province, cambodia. all collected fish with ice were transferred to our laboratory and examined using the artificial digestion method. in fish from phnom penh, 2 kinds of metacercariae (opisthorchis viverrini and haplorchis yokogawai) were detected. o. viverrini metacercariae were positive in 37 (50.0%) of 74 fish in 11 species (average no. metacercariae/fi ... | 2014 | 24623879 |
| first insight into cd59-like molecules of adult fasciola hepatica. | the present study focussed on investigating cd59-like molecules of fasciola hepatica. a cdna encoding a cd59-like protein (termed fhcd59-1) identified previously in the membrane fraction of the f. hepatica tegument was isolated. this homologue was shown to encode a predicted open reading frame (orf) of 122 amino acids (aa) orthologous to human cd59 with a 25 aa signal peptide, a mature protein containing 10 cysteines and a conserved cd59/ly-6 family motif "ccxxxxcn". an analysis of cdnas from tw ... | 2014 | 24955521 |
| cytokine/chemokine secretion and proteomic identification of upregulated annexin a1 from peripheral blood mononuclear cells cocultured with the liver fluke opisthorchis viverrini. | we investigated the cytokine/chemokine secretions and alteration of protein expression from peripheral blood mononuclear cells (pbmcs) cocultured with adult liver flukes (opisthorchis viverrini) for 6 to 24 h. pbmc-derived proteins were identified by two-dimensional electrophoresis and mass spectrometry, and the cytokines/chemokines in the supernatant were assessed using a cytokine array. exposure to o. viverrini induced increases in secretion of proinflammatory cytokines, costimulating protein, ... | 2014 | 24614660 |
| prevalence of intestinal helminths among inhabitants of cambodia (2006-2011). | in order to investigate the status of intestinal helminthic infections in cambodia, epidemiological surveys were carried out on a national scale, including 19 provinces. a total of 32,201 fecal samples were collected from schoolchildren and adults between 2006 and 2011 and examined once by the kato-katz thick smear technique. the overall egg positive rate of intestinal helminths was 26.2%. the prevalence of hookworms was the highest (9.6%), followed by that of opisthorchis viverrini/minute intes ... | 2014 | 25548418 |
| development of a pcr assay and pyrosequencing for identification of important human fish-borne trematodes and its potential use for detection in fecal specimens. | small liver and minute intestinal flukes are highly prevalent in southeast asia. definitive diagnosis of parasite infection is usually achieved parasitologically by finding the fluke eggs in feces. however, their eggs are difficult to differentiate morphologically in fecal samples, even for experienced technicians. the present study developed a pcr assay coupled with dna pyrosequencing for identification of the fish-borne trematodes (fbt), opisthorchis viverrini, clonorchis sinensis, haplorchis ... | 2014 | 24589167 |
| prevalence of helminthic infections among inhabitants of lao pdr. | the prevalence of helminthic infections was surveyed on a total of 6,178 residents (males 2,549 and females 3,629) in 102 villages of 9 provinces in lao pdr between 2007 and 2011 under the project of korea-laos collaborative project for control of foodborne trematode infections in lao pdr. fecal specimens were collected and examined by the kato-katz thick smear and stoll's egg counting techniques. the overall liver/intestinal helminth egg positive rate was 71.9% with a single or mixed infections ... | 2014 | 24623882 |
| roles of liver fluke infection as risk factor for cholangiocarcinoma. | several factors are known to be associated with risk of cholangiocarcinoma (cca) and infection with the liver flukes, opisthorchis viverrini and clonorchis sinensis, has often been singled out as the leading risk factor in east and southeast asia. in this review, current knowledge of their biology, life cycle, and pathogenesis of o. viverrini, and its role as a carcinogenic parasite are presented. the trends of age-specific incidence of liver cancer in khon kaen, northeast thailand are considere ... | 2014 | 24408775 |
| retrotransposon ov-rte-1 from the carcinogenic liver fluke opisthorchis viverrini: potential target for dna-based diagnosis. | infections by the fish-borne liver flukes opisthorchis viverrini and clonorchis sinensis can lead to bile duct cancer. these neglected tropical disease pathogens occur in east asia, with o. viverrini primarily in thailand and laos and c. sinensis in cambodia, vietnam, and china. genomic information about these pathogens holds the potential to improve disease treatment and control. transcriptome analysis indicates that mobile genetic elements are active in o. viverrini, including a novel non-long ... | 2014 | 24394447 |
| hepatitis b virus infection and intrahepatic cholangiocarcinoma. | intrahepatic cholangiocarcinoma (icc) is a devastating malignant tumor arising from the peripheral intrahepatic bile duct epithelium. the incidence and mortality of icc is markedly increasing over the past two decades worldwide, though the cause for this rise in incidence is unclear, thus intensifying the search for alternative etiological agents and pathogenetic mechanisms. hepatolithiasis, primary sclerosing cholangitis, parasitic infection (opisthorchis viverrini or clonorchis sinensis), fibr ... | 2014 | 24914333 |
| opisthorchis viverrini: analysis of the sperm-specific rhophilin associated tail protein 1-like. | concurrent deficiency of rhophilin associated tail protein (ropn1) and ropn1-like (ropn1l) in mice causes structural abnormalities and immotility of sperm and thereby infertility. in the present research, ropn1l of the human liver fluke opisthorchis viverrini was molecularly characterized and showed unexpected potential as a diagnostic tool. ropn1l transcripts were detected in 2-week-old juveniles by rt-pcr. immunohistochemical analysis of the adult worm localized the protein in testis lobes, se ... | 2014 | 25124046 |
| prevalence of infection and molecular confirmation by using its-2 region of fasciola gigantica found in domestic cattle from chiang mai province, thailand. | to investigate the infection of fasciola gigantica (f. gigantica) in domestic cattle from chiang mai province and molecular confirmation using its-2 region. | 2014 | 24507641 |
| effects of melatonin on oxidative stress, and resistance to bacterial, parasitic, and viral infections: a review. | melatonin, a hormone secreted by the pineal gland, works directly and indirectly as a free radical scavenger. its other physiological or pharmacological activities could be dependent or independent of receptors located in different cells, organs, and tissues. in addition to its role in promoting sleep and circadian rhythms regulation, it has important immunomodulatory, antioxidant, and neuroprotective effects suggesting that this indole must be considered as a therapeutic alternative against inf ... | 2014 | 24811367 |
| prevalence of haplorchis taichui among humans and fish in luang prabang province, lao pdr. | this study confirmed the prevalence of the intestinal fluke haplorchis taichui (trematoda: heterophyidae) among people and fish in luang prabang province, lao pdr. fecal specimens were collected from 559 riparian people (229 males and 330 females), residing in 4 districts (luang prabang, xieng ngeun, pak ou, and nam bak) and were examined by the kato-katz fecal smear technique. the overall helminth egg positive rate was 64.9%. the positive rate for small trematode eggs (ste), which may include h ... | 2014 | 24754919 |
| the mirnaome of opisthorchis viverrini induced intrahepatic cholangiocarcinoma. | intrahepatic cholangiocarcinoma (icc) is an aggressive cancer, arising in the biliary ducts that extend into the liver. the highest incidence of icc occurs in southeast asia, particularly in the mekong river basin countries of thailand, laos, cambodia, and vietnam, where it is strongly associated with chronic infection by the food-borne liver fluke opisthorchis viverrini (ov), one of only three eukaryote pathogens considered group one carcinogens. intrahepatic cholangiocarcinoma is usually diagn ... | 2014 | 26484108 |
| suppression of aquaporin, a mediator of water channel control in the carcinogenic liver fluke, opisthorchis viverrini. | opisthorchiasis and opisthorchis viverrini-associated bile duct cancer represent major public health threats in thailand and laos. the tegument of this food borne fluke plays pivotal roles in parasite metabolism, homeostasis and osmoregulation. excretory/secretory products also pass from the fluke to the biliary environment, products that likely underlie pathogenesis of liver fluke infection. aquaporins (aqps), belong to the major intrinsic protein superfamily of integral plasma membrane channel ... | 2014 | 24885060 |
| genomic and transcriptional alterations of cholangiocarcinoma. | cholangiocarcinoma (cca) is one of the representative cancers refractory to any therapeutic approach. the incidence of cca is highest in the northeastern part of thailand, where chronic inflammation caused by liver fluke (opisthorchis viverrini: ov) infection is a major etiologic factor. the incidence of cca is also increasing in other countries, including japan. here, we overview the genetic and transcriptional alterations of cca with and without association with ov infection. cca with ov shows ... | 2014 | 24532422 |
| potential targeted therapy for liver fluke associated cholangiocarcinoma. | biliary tree cancer or cholangiocarcinoma (cca) is an unusual subtype of liver cancer with exceptionally poor prognosis. lack of specific symptoms and availability of early diagnostic markers account for late diagnosis of cca. surgical treatment is a gold standard choice but few patients are candidates and local recurrence after surgery is high. benefit of systemic chemotherapy is limited; hence, better treatment options are required. the differences in etiology, anatomical positions and patholo ... | 2014 | 24408866 |
| risk biomarkers for assessment and chemoprevention of liver fluke-associated cholangiocarcinoma. | human liver fluke, opisthorchis viverrini (ov), is the major risk factor of cholangiocarcinoma (cca) in northeastern thailand. our approach focuses on genetic progression and molecular changes in the carcinogenic pathway of liver fluke-associated cca aimed at assessing patients at risk of cca and using chemoprevention as the secondary cancer prevention to reduce the incidence of cca. this review summarizes altered gene expressions, biomolecules and their modification, i.e. dna adducts, oxidized ... | 2014 | 24408859 |
| bile canalicular changes and defective bile secretion in opisthorchis viverrini-infected hamsters. | infection with the liver fluke opisthorchis viverrini (digenea) (poirier, 1886) causes bile duct injury and periductal fibrosis by chronic overproduction of inflammatory-mediators and eventually results in cholangiocarcinoma development. while extensive research works have been done on o. viverrini infection-associated changes of bile ducts and periductal fibrosis, little attention was paid on morphological and biochemical changes of the bile canaliculi (bc), the origin of bile flow. we aimed to ... | 2014 | 25651692 |
| opisthorchis viverrini infection activates the pi3k/ akt/pten and wnt/β-catenin signaling pathways in a cholangiocarcinogenesis model. | opisthorchis viverrini (ov) infection is the major etiological factor for cholangiocarcinoma (cca), especially in northeast thailand. we have previously reported significant involvement of pi3k/akt/pten and wnt/β- catenin in human cca tissues. the present study, therefore, examined the expression and activation of pi3k/ akt/pten and wnt/β-catenin signaling components during ov-induced cholangiocarcinogenesis in a hamster animal model. hamsters were divided into two groups; non-treated and ov plu ... | 2014 | 25556493 |
| genetic and environmental determinants of risk for cholangiocarcinoma in thailand. | cholangiocarcinoma (cca) is a difficult cancer to diagnose in the early stage and to treat by curative resection. the incidence of cca in the northeast of thailand is the highest in the world. to make progress in detecting a high risk group and in the prevention and detection of cca, we have been analyzing the risk factors for cca. although liver fluke infection is known to be a risk factor, there are patients who are not infected with the liver fluke and not all people infected with the liver f ... | 2014 | 25401000 |
| circulating mir-192 in liver fluke-associated cholangiocarcinoma patients: a prospective prognostic indicator. | this study aimed to investigate the mir-192 levels in patients' sera of liver fluke-associated cholangiocarcinoma (cca) for a prospective prognostic indicator. | 2014 | 25131257 |
| stats profiling reveals predominantly-activated stat3 in cholangiocarcinoma genesis and progression. | we investigated the aberrant expression of the stat family in humans and liver fluke (opisthorchis viverrini, ov)-induced hamster cholangiocarcinoma (cca) tissues. | 2014 | 25044480 |
| an interleukin-6 receptor polymorphism is associated with opisthorchiasis-linked cholangiocarcinoma risk in thailand. | the cholangiocarcinoma (cca) is a relatively rare cancer worldwide but it is highly prevalent in thailand where the liver fluke, opisthorchis viverrini is endemic. there are reports that interleukin 6 (il-6) may play an important role in the pathogenesis of opisthorchiasis associated cca. functionally, il-6 can act on target cells through its receptor, il-6r, and il-6r polymorphisms may affect the functional activity of il-6 leading to susceptibility to cholangiocarcinogenesis. therefore, we ass ... | 2014 | 25041016 |
| distinct mirna signatures associate with subtypes of cholangiocarcinoma from infection with the tumourigenic liver fluke opisthorchis viverrini. | intrahepatic cholangiocarcinoma (icc) is a significant public health problem in east asia, where it is strongly associated with chronic infection by the food-borne parasite opisthorchis viverrini (ov). we report the first comprehensive mirna expression profiling by microarray of the most common histologic grades and subtypes of icc: well differentiated, moderately differentiated, and papillary icc. | 2014 | 25017828 |
| opisthorchis viverrini-like liver fluke in birds from vietnam: morphological variability and rdna/mtdna sequence confirmation. | flukes were found in the bile ducts of domestic ducks (anas platyrhynchos), necropsied in the binh dinh province of central vietnam. following staining, morphological characteristics of the bird flukes were compatible with opisthorchis viverrini, although some characteristics differed from those described in specimens collected from mammal hosts. computation of the phylogenetic trees on the partial sequences of the second internal ribosomal spacer (its2) of the ribosomal dna and cytochrome c oxi ... | 2014 | 23721954 |
| endemicity of opisthorchis viverrini liver flukes, vietnam, 2011-2012. | 2014 | 24377371 | |
| rna-seq reveals infection-induced gene expression changes in the snail intermediate host of the carcinogenic liver fluke, opisthorchis viverrini. | bithynia siamensis goniomphalos is the snail intermediate host of the liver fluke, opisthorchis viverrini, the leading cause of cholangiocarcinoma (cca) in the greater mekong sub-region of thailand. despite the severe public health impact of opisthorchis-induced cca, knowledge of the molecular interactions occurring between the parasite and its snail intermediate host is scant. the examination of differences in gene expression profiling between uninfected and o. viverrini-infected b. siamensis g ... | 2014 | 24676090 |
| the opisthorchis viverrini genome provides insights into life in the bile duct. | opisthorchiasis is a neglected, tropical disease caused by the carcinogenic asian liver fluke, opisthorchis viverrini. this hepatobiliary disease is linked to malignant cancer (cholangiocarcinoma, cca) and affects millions of people in asia. no vaccine is available, and only one drug (praziquantel) is used against the parasite. little is known about o. viverrini biology and the diseases that it causes. here we characterize the draft genome (634.5 mb) and transcriptomes of o. viverrini, elucidate ... | 2014 | 25007141 |
| chronic opisthorchis viverrini infection and associated hepatobiliary disease is associated with iron loaded m2-like macrophages. | chronic opisthorchis viverrini-induced hepatobiliary disease is associated with significant leukocyte infiltration, including activated macrophages; however, the polarization of infiltrating macrophages remains to be fully characterized. in this study, we characterized macrophage polarization and phenotype in chronic o. viverrini-induced hepatobiliary disease in humans and hamsters using gene expression and histochemical analysis. chronic o. viverrini infection and associated hepatobiliary disea ... | 2014 | 25548425 |
| anti-inflammatory and anti-periductal fibrosis effects of an anthocyanin complex in opisthorchis viverrini-infected hamsters. | the pharmacological activities of herbal extracts can be enhanced by complex formation. in this study, we manipulated cyanidin and delphinidin-rich extracts to form an anthocyanin complex (ac) with turmeric and evaluated activity against inflammation and periductal fibrosis in opisthorchis viverrini-infected hamsters. the ac was prepared from anthocyanins extracted from cobs of purple waxy corn (70%), petals of blue butterfly pea (20%) and turmeric extract (10%), resulting in an enhanced free-ra ... | 2014 | 25447758 |
| analysis of new biomarkers for cholangiocarcinoma. | cholangiocarcinoma is one of the most serious diseases in northeast thailand, where its incidence is reported to be the highest in the world. we tried to develop a new method to detect cholangiocarcinoma in the early stages using serum proteins. we found that after fluorescent labeling of the sugar moiety of serum proteins, a new peak was identified, which might be a promising marker for cholangiocarcinoma. | 2014 | 24446393 |
| histological confirmation of periductal fibrosis from ultrasound diagnosis in cholangiocarcinoma patients. | cholangiocarcinoma (cca) has no specific clinical signs and symptoms and non-specific bio- and tumor-markers in the early disease stage. usually patients present to tertiary care with advanced disease stage. in order to detect early cases of cca that may present as a mass, dilatation of intrahepatic duct or combination, ultrasonography is accepted as a powerful imaging tool. a smaller mass or bile duct segmental dilatation requires further imaging for characterization, including computerized tom ... | 2014 | 24420706 |
| prevalence and risk factors for opisthorchis viverrini infections in upper northeast thailand. | opisthorchis viverrini is an ongoing public health problem in northeast thailand. despite continuous efforts for decades by healthcare organizations to overcome this problem, infection rates remain high. to enable related personnel to identify and address the various issues effectively, a cross-sectional study was performed to investigate prevalence and risk factors for opisthorchiasis. the target group was 3,916 thai residents of northeast thailand who were 15 or over. participants were recruit ... | 2014 | 25169496 |
| analysis of the population genetics of opisthorchis viverrini sensu lato in the nam ngum river wetland, lao pdr, by multilocus enzyme electrophoresis. | a previous population genetics study of opisthorchis viverrini from a locality in an endemic area in thailand found little genetic variation over time and second intermediate fish host species. since a similar comparative analysis is not available for lao pdr, we conducted a study of o. viverrini from different endemic foci in vientiane province, lao pdr, based on spatial, temporal and fish host species. a total of 620 adult o. viverrini originating from the nam ngum river wetland were analysed ... | 2014 | 25033811 |
| risk factors for opisthorchis viverrini and minute intestinal fluke infections in lao pdr, 2009-2011. | the purpose of this report is to compare the prevalence of opisthorchis viverrini/minute intestinal fluke (ov/mif) in lao people's democratic republic according to different regions and evaluate the effect of ingestion of raw freshwater fish on the infection. demographic data, behavioral data, and symptoms were obtained, and the kato-katz method was used for the diagnosis of ov/mif. in total, 3,046 individuals were enrolled. the risk factors associated with acquisition of ov/mif were raw fish co ... | 2014 | 24980495 |
| localization of the cystogenous glands of opisthorchis viverrini cercariae. | opisthorchiasis in northeastern thailand is an important etiology of cholangiocarcinoma. to form the infectious stage, free swimming cercariae penetrate cyprinid fish, shed their tails, and then secret a cystic substance to cover their larval stage to form metacercariae in the fish body. we determined the location of the cystogenous glands in opisthorchis viverrini cercariae. the cercariae and metacercariae were obtained from the naturally infected snail host, bithynia siamensis goniomphalos and ... | 2014 | 24968667 |
| experimental infection of opisthorchis viverrini cercariae to the cyprinid fish, barbonymus gonionotus. | opisthorchis viverrini is an important public health problem, a major cause of cholangiocarcinoma in the greater mekong subregion including lao pdr, cambodia, vietnam and thailand. humans acquire the infection by consumption of raw, fermented or partially cooked freshwater cyprinid fish containing infective metacercariae. this study examined the effect of cercarial infection dosage (25-200 cercariae), age (1-60 day) and size (1-24mm) of barbonymus gonionotus fish on infection success of o. viver ... | 2014 | 24780189 |
| intestinal parasitic infections in hiv-infected patients, lao people's democratic republic. | hiv infection is an emerging problem in laos. we conducted the first prospective study on intestinal parasites, including opportunistic protozoa, in newly diagnosed hiv infected patients, with or without diarrhea. the aims were to describe the spectrum of infections, to determine their prevalence and to assess their associations with diarrhea, cd4 cell count, place of residence and living conditions. | 2014 | 24662743 |
| seasonal cercarial emergence patterns of opisthorchis viverrini infecting bithynia siamensis goniomphalos from vientiane province, lao pdr. | snail intermediate hosts play a pivotal role in maintaining the life cycles of trematodes, including opisthorchis viverrini. we investigated the emergence patterns of o. viverrini cercariae infecting bithynia siamensis goniomphalos at foci in an endemic area in vientiane province, lao pdr. | 2014 | 25442515 |
| traditional herbal medicine for the control of tropical diseases. | throughout history, traditional herbal medicine has afforded a rich repository of remedies with diverse chemical structures and bioactivities against several health disorders. a common issue of herbal medicine is the limitation of information on their pharmacological activities and their active constituents. traditionally, the use of herbal medicine has been based on empirical treatment and passed on from generation to generation with information available only in local journals. this prevents s ... | 2014 | 25425945 |
| selective immunostaining of mehlis' gland of opisthorchis viverrini by antibody against rat diacylglycerol kinase. | the mehlis' gland of opisthorchis viverrini was selectively and intensely immunopositive with an antibody against rat diacylglycerol kinase gamma, and its entire structure with associated radiating processes was clearly demonstrated by immuno-light microscopy. in immuno-electron microscopy, the immunopositive processes were revealed to contain many vesicles and vacuoles and the immunoreactive materials were deposited diffusely in the cytoplasm except for the vesicular interior. the present findi ... | 2014 | 25417499 |
| gene-environment interaction involved in cholangiocarcinoma in the thai population: polymorphisms of dna repair genes, smoking and use of alcohol. | cholangiocarcinoma (cca) is the most common malignancy in a northeast thai population. smoking and alcohol drinking are associated with the production of free radical intermediates, which can cause several types of dna lesions. reduced repair of these dna lesions would constitute an important risk factor for cancer development. we therefore examined whether polymorphisms in dna base-excision repair (ber) genes, xrcc1 g399a and ogg1 c326g, were associated with cca risk and whether they modified t ... | 2014 | 25335960 |
| increase of exostosin 1 in plasma as a potential biomarker for opisthorchiasis-associated cholangiocarcinoma. | a proteomic-based approach was used to search for potential markers in the plasma of hamsters in which cholangiocarcinoma (cca) was induced by opisthorchis viverrini infection and n-nitrosodimethylamine treatment. the plasma proteins of cca-induced hamsters were resolved by 1-d page, digested by trypsin, and analyzed by lc-ms/ms. from the criteria of protein id scores >15 and an overexpression of at least three times across all time points, 37 proteins were selected. these overexpressed proteins ... | 2014 | 24018821 |
| sex differences in opisthorchiosis and the development of cholangiocarcinoma in syrian hamster model. | worldwide, the highest incidence of cholangiocarcinoma (cca) is found in northeast thailand, the endemic area of opisthorchis viverrini infection. cumulated clinical data revealed that the majority of cca patients are men. however, many other types of cancers are more commonly found in women. in this study, we investigated the sex differences in the development of cca, induced by o. viverrini infection and n-nitrosodimethylamine administration, in syrian hamsters. histopathology, liver function ... | 2014 | 24318666 |
| synergistic effects of isomorellin and forbesione with doxorubicin on apoptosis induction in human cholangiocarcinoma cell lines. | chemotherapy for advanced cholangiocarcinoma (cca) is largely ineffective, but innovative combinations of chemotherapeutic agents and natural compounds represent a promising strategy. in our previous studies, isomorellin and forbesione, caged xanthones isolated from garcinia hanburyi, were found to induce cell cycle arrest and apoptosis in cca cell lines. the subject of our inquiry is the synergistic effect(s) of these caged xanthones with doxorubicin on growth inhibition and apoptosis induction ... | 2014 | 25866479 |
| field survey focused on opisthorchis viverrini infection in five provinces of cambodia. | opisthorchiasis is endemic in thailand and lao people's democratic republic and constitutes a major public health problem throughout the mekong basin. although cambodia is located in the mekong basin, the status of o. viverrini infection in that country was not previously clarified. this research was conducted to document the extent and distribution of o. viverrini infection in cambodia. | 2014 | 24342554 |
| milk fat globule epidermal growth factor 8 serves a novel biomarker of opisthorchiasis-associated cholangiocarcinoma. | milk fat globule epidermal growth factor 8 (mfg-e8) is a pleiotropic secreted glycoprotein to play roles in mediating immune tolerance and homeostasis maintenance and enhancing angiogenesis. to evaluate its value as a biomarker in opisthorchiasis-associated cholangiocarcinoma (cca), the present study investigated mfg-e8 expression kinetics during the tumorigenesis in opisthorchis viverrini infection-induced cca, and demonstrated its expression in the tumor tissues of cca patients and its serum l ... | 2014 | 24122204 |
| development and evaluation of pcr methods based on cytochrome c oxidase subunit one (cox1) and nadh dehydrogenase subunit one gene (nad1) to detect opisthorchis viverrini in human fecal samples. | opisthorchis viverrini is highly prevalent throughout southeast asia. chronic infection of this parasite leads to cholangiocarcinoma (cca), a fatal bile duct cancer. the early and accurate detection of this parasite is very important; therefore, new pcr methods targeting cytochrome c oxidase subunit one and nadh dehydrogenase subunit one gene to detect o. viverrini in fecal specimens have been developed. ninety o. viverrini-positive human fecal samples were used in this study. the pcr sensitivit ... | 2015 | 26239798 |
| why does infection with some helminths cause cancer? | infections with opisthorchis viverrini, clonorchis sinensis, and schistosoma haematobium are classified as group 1 biological carcinogens: definitive causes of cancer. these worms are metazoan eukaryotes, unlike the other group 1 carcinogens including human papilloma virus, hepatitis c virus, and helicobacter pylori. by contrast, infections with phylogenetic relatives of these helminths, also trematodes of the phylum platyhelminthes and major human pathogens, are not carcinogenic. these inconsis ... | 2015 | 28741473 |
| temperature dependence of opisthorchis viverrini infection in first intermediate host snail, bithynia siamensis goniomphalos. | determining of the success of a parasite's infectiveness in its snail host clearly depends on environmental conditions. temperature, one of the most influential factors impinging on metabolism of cold-blooded animals, is believed to be an important factor in parasitic infection in snails. in order to elucidate the influence of temperature, sex and size of snails on infectivity of opisthorchis viverrini to its first intermediate host, bithynia siamensis goniomphalos, 960 snails were divided into ... | 2015 | 24161535 |
| bithynia siamensis goniomphalos, the first intermediate host of opisthorchis viverrini in thailand. | opisthorchiasis caused by opisthorchis viverrini (o. viverrini) remains as medically important problem in thailand especially in the north-eastern part. infection with this parasite can lead to cholangiocarcinoma improvement. the highest prevalence of o. viverrini infection has been found in the northeast thailand and is associated with the high incidence rate of cholangiocarcinoma. to complete the life cycle of o. viverrini, the freshwater snails namely bithynia funiculata, bithynia siamensis s ... | 2015 | 26522291 |
| trematode infection of freshwater snail, family bithyniidae in thailand. | opisthorchis viverrini is restricted to and requires for its aquatic life cycle only bithynia snail as first intermediate host but many species of cyprinid fish as second intermediate hosts. a survey in thailand of trematode infection in freshwater snails of the family bithyniidae carried out during october 2008 - july 2009 found a total of 5,492 snails, classified into ten species distributed in various geographic areas. bithyniafuniculata and gabbia pygmaea were localized to the north, b. s. g ... | 2015 | 26521513 |
| community participation in cholangiocarcinoma prevention in ubon ratchathani, thailand: relations with age and health behavior. | a high prevalence of opisthorchis viverrini infection is usually found in wetland geographical areas of thailand where people have traditional behavior of eating uncooked freshwater fish dishes which results in cholangiocarcinoma (cca) development. there were several approaches for reducing opisthorchiasis-linked cca, but the prevalence remains high. to develop community participation as a suitable model for cca prevention is, firstly, to know what factors are related. we therefore aimed to inve ... | 2015 | 26514539 |
| review and current status of opisthorchis viverrini infection at the community level in thailand. | opisthorchis viverrini is remains a public health problem in thailand, particularly in the northeast and north regions which have the highest incidences of chonalgiocarcinoma (cca). o. viverrini causes the disease opithorchiasis, and its has been classified as a group 1 biological carcinogen. humans, dogs, and cats become infected with o. viverrini by ingesting raw or undercooked fish containing infective metacercariae. the first human cases of o. viverrini infection were reported in thailand 10 ... | 2015 | 26514452 |
| human trichostrongyliasis: a hospital case series. | trichostrongylus is a common nematode found to infect livestock throughout the tropics and can cause accidental zoonosis in humans. in the lao pdr and thailand, cases of human trichostrongyliasis have been reported sporadically but clinical data are limited. we retrospectively reviewed 41 cases of trichostrongyliasis who presented to srinagarind hospital, thailand from 2005 to 2012. the diagnosis of trichostrongyliasis was made by finding their eggs in the stool of patients. of the 41 cases revi ... | 2015 | 26513921 |
| anticancer activity of atractylodes lancea (thunb.) dc in a hamster model and application of pet-ct for early detection and monitoring progression of cholangiocarcinoma. | opisthorchis viverrini (ov)-induced cholangiocarcinoma (cca) is an important cancer in the great mekong region, particularly in thailand. limitations of treatment options and the lack of an effective diagnostic tool for early detection of cca are major concerns for the control of this type of cancer. the aim of the study was to investigate anti-cca activity of the ethanolic extract of atractylodes lancea (thunb.) dc., and the applicability of positron emission tomography-computed tomography (pet ... | 2015 | 26434829 |
| levels of 8-oxodg predict hepatobiliary pathology in opisthorchis viverrini endemic settings in thailand. | opisthorchis viverrini is distinct among helminth infections as it drives a chronic inflammatory response in the intrahepatic bile duct that progresses from advanced periductal fibrosis (apf) to cholangiocarcinoma (cca). extensive research shows that oxidative stress (os) plays a critical role in the transition from chronic o. viverrini infection to cca. os also results in the excision of a modified dna lesion (8-oxodg) into urine, the levels of which can be detected by immunoassay. herein, we m ... | 2015 | 26230769 |
| multiparasitism and intensity of helminth infections in relation to symptoms and nutritional status among children: a cross-sectional study in southern lao people's democratic republic. | the occurrence and spatial distribution of intestinal helminth infection in children is fairly well understood. however, knowledge on how helminth infections govern intestinal morbidity is scarce. we conducted a cross-sectional study to assess and quantify the relationship between single and multiple species helminth infection with clinical and self-reported morbidity indicators and nutritional status in champasack province, southern lao people's democratic republic (lao pdr). a random sample of ... | 2015 | 25291046 |
| mri and (1)h mrs findings of hepatobilary changes and cholangiocarcinoma development in hamsters infected with opisthorchis viverrini and treated with n-nitrosodimethylamine. | 3 t mri and (1)h mrs were useful for quantitative investigation of the serial development of hepatobiliary changes in opisthorchis viverrini infection in hamsters, and the differential diagnosis of cholangiocacinoma (cca) development from bile duct changes and normal condition is unclear. in this study, we investigated the serial development of hepatobiliary changes and ccagenesis in o. viverrini-infected and n-nitrosodimethylamine (ndma) treated hamsters (on group) using 3 t mri and (1)h mrs an ... | 2015 | 26117689 |
| risk factors for opisthorchis viverrini infection in nong khai province, thailand. | opisthorchis viverrini (ov) infection is the main risk factor for cholangiocarcinoma and is often found in northeastern thailand. the prevalence of ov infection and the incidence of cholangiocarcinoma are major public health problems in this region. | 2015 | 26107209 |
| lectin microarray-based sero-biomarker verification targeting aberrant o-linked glycosylation on mucin 1. | glycoform of mucin 1 (muc1) in cancerous cells changes markedly with cell differentiation, and thus, qualitative detection and verification of the muc1 glycosylation changes have potential diagnostic value. we have developed an ultrasensitive method to detect the changes in cholangiocarcinoma (cc), which produces muc1, and applied it in the diagnostics development. the focused glycan analysis using 43-lectin-immobilized microarray could obtain the glycan profiles of sialylated muc1 in 5 μl of se ... | 2015 | 26091356 |
| lc-ms/ms method for the determination of two metabolites of tribendimidine, deacylated amidantel and its acetylated metabolite in plasma, blood and dried blood spots. | tribendimidine has emerged as potential alternative to praziquantel in the treatment of opisthorchis viverrini infections. to support its clinical development program, a quantitative high performance liquid chromatography-tandem mass spectrometry (hplc-ms/ms) assay was developed for tribendimidine's degradation product deacylated amidantel (dadt) and its acetylated metabolite adadt. analytical sample preparation included protein precipitation for blood and plasma, and direct processing of dried ... | 2015 | 25553533 |
| prevalence and risk factors for infection by opisthorchis viverrini in an urban area of mahasarakham province, northeast thailand. | the aim of this cross-sectional analytic study was to investigate the prevalence and risk factors for opisthorchis viverrini (ov) infection in an urban area of northeastern thailand. the participants were 254 household representatives aged 15 years or older living in the most urbanised part of chiang yuen municipality in mahasarakham province. all participants provided stool samples which were examined using the modified kato-katz procedure, and a structured interview questionnaire was used to c ... | 2015 | 26028068 |
| seasonal transmission of opisthorchis viverrini sensu lato and a lecithodendriid trematode species in bithynia siamensis goniomphalos snails in northeast thailand. | seasonal changes play roles in the transmission success of fish-borne zoonotic trematodes (fzt). this study examined the seasonal transmission patterns of opisthorchis viverrini sensu lato (s.l.) and a virgulate cercaria (family lecithodendriidae) in the snail intermediate host, bithynia siamensis goniomphalos in northeast thailand. snail samples were collected monthly during the rainy, cool, and hot seasons during 2012-2013 to determine the prevalence and intensity of larval trematode infection ... | 2015 | 25918210 |
| a microrna profile associated with opisthorchis viverrini-induced cholangiocarcinoma in tissue and plasma. | intrahepatic cholangiocarcinoma (icc) is a highly aggressive tumor of the bile duct, and a significant public health problem in east asia, where it is associated with infection by the parasite opisthorchis viverrini. icc is often detected at an advanced stage and with a poor prognosis, making a biomarker for early detection a priority. | 2015 | 25903557 |
| quantitative changes in tumor-associated m2 macrophages characterize cholangiocarcinoma and their association with metastasis. | the tumor microenvironment (tme) includes numerous non-neoplastic cells such as leukocytes and fibroblasts that surround the neoplasm and influence its growth. tumor-associated macrophages (tams) and cancer-associated fibroblasts (cafs) are documented as key players in facilitating cancer appearance and progression. alteration of the macrophage (cd68, cd163) and fibroblast (α-sma, fsp-1) cells in opisthorchis viverrini (ov)-induced cholangiocarcinoma (cca) was here assessed using liver tissues f ... | 2015 | 25854403 |
| plasma phosphoproteome and differential plasma phosphoproteins with opisthorchis viverrini-related cholangiocarcinoma. | this study was conducted to investigate the plasma phosphoproteome and differential plasma phosphoproteins in cases of of opisthorchis viverrini (ov)-related cholangiocarcinoma (cca). plasma phosphoproteomes from cca patients (10) and non-cca subjects (5 each for healthy subjects and ov infection) were investigated using gel-based and solution-based lc-ms/ms. phosphoproteins in plasma samples were enriched and analyzed by lc-ms/ms. strap, panther, ipath, and mev programs were applied for the ide ... | 2015 | 25735322 |
| reprint of "experimental infection of opisthorchis viverrini cercariae to the cyprinid fish, barbonymus gonionotus". | opisthorchis viverrini is an important public health problem, a major cause of cholangiocarcinoma in the greater mekong subregion including lao pdr, cambodia, vietnam and thailand. humans acquire the infection by consumption of raw, fermented or partially cooked freshwater cyprinid fish containing infective metacercariae. this study examined the effect of cercarial infection dosage (25-200 cercariae), age (1-60 day) and size (1-24 mm) of barbonymus gonionotus fish on infection success of o. vive ... | 2015 | 25446173 |
| [role of opisthorchis felineus on induction of bile duct cancer]. | opisthorchis felineus (trematoda) is widespread in the russian federation, especially in siberia, and other countries of europe. infestation of endemic area population with o. felineus reaches 80%. on animal models of the infection of closely related opisthorchis viverrini combined with the nitrosamines' intake it has been shown that the parasite induces cholangiocarcinoma. however carcinogenic potential of o. felineus is still poorly studied. the present study is aimed to investigate the role o ... | 2015 | 26016330 |
| response to manuscript 'is opisthorchis viverrini an avian liver fluke?'. | 2015 | 24160764 | |
| is opisthorchis viverrini an avian liver fluke? | recently, in the journal of helminthology (may 2013), dao et al. reported that opisthorchis viverrini-like flukes were found in the bile duct of domestic ducks in vietnam. they stated that this is the first record of opisthorchis sp. in birds in vietnam. however, three opisthorchis species--o. cheelis, o. longissimus and o. parageminus--in birds in vietnam were described by le in 2000. amongst these, o. parageminus was first reported, by oshmarin in 1970, as a new opisthorchis species found in d ... | 2015 | 24160690 |
| excretory/secretory products of the carcinogenic liver fluke are endocytosed by human cholangiocytes and drive cell proliferation and il6 production. | liver fluke infection caused by opisthorchis viverrini remains a major public health problem in many parts of asia including thailand, lao pdr, vietnam and cambodia, where there is a strikingly high incidence of cholangiocarcinoma (cca - hepatic cancer of the bile duct epithelium). among other factors, uptake of o. viverrini excretory/secretory products (oves) by biliary epithelial cells has been postulated to be responsible for chronic inflammation and proliferation of cholangiocytes, but the m ... | 2015 | 26187786 |
| apoptosis of cholangiocytes modulated by thioredoxin of carcinogenic liver fluke. | chronic infection with the food-borne liver fluke, opisthorchis viverrini, frequently induces cancer of the bile ducts, cholangiocarcinoma. opisthorchiasis is endemic in thailand, lao pdr, cambodia and vietnam, where eating undercooked freshwater fish carrying the juvenile stage of this pathogen leads to human infection. because inhibition of apoptosis facilitates carcinogenesis, this study investigated modulation by thioredoxin from o. viverrini of apoptosis of bile duct epithelial cells, chola ... | 2015 | 26007234 |
| diabetes mellitus: possible risk and promoting factors of cholangiocarcinoma: association of diabetes mellitus and cholangiocarcinoma. | the highest incidence of cholangiocarcinoma (cca), a malignancy of bile duct epithelia, is in the northeast of thailand. the liver fluke, opisthorchis viverrini, is the known risk factor for cca development in this region. approximately 1% of o. viverrini infected individuals develop cca. there could be other factors that influence the cholangiocarcinogenesis particularly in the o. viverrini infected individuals. the global epidemiological studies of risk factors for cca in non-o. viverrini rela ... | 2015 | 25910864 |
| data set from the proteomic analysis of bithynia siamensis goniomphalos snails upon infection with the carcinogenic liver fluke opisthorchis viverrini. | the snail bithynia siamensis goniomphalos acts as the first intermediate host for the human liver fluke opisthorchis viverrini, the major cause of cholangiocarcinoma (cca) in northeast thailand. this data article contains the results obtained from the analysis of the proteins differentially expressed in the snail b. siamensis goniomphalos upon infection with o. viverrini. it contains the data generated from iquantitator software including a pdf of each sample with a protein׳s relative expression ... | 2015 | 26217698 |
| toward integrated opisthorchiasis control in northeast thailand: the lawa project. | human liver fluke, opisthorchis viverrini, a food-borne trematode is a significant public health problem in southeast asia, particularly in thailand. despite a long history of control programmes in thailand and a nationwide reduction, o. viverrini infection prevalence remains high in the northeastern provinces. therefore, a new strategy for controlling the liver fluke infection using the ecohealth/one health approach was introduced into the lawa lake area in khon kaen province where the liver fl ... | 2015 | 25102053 |
| risk areas of liver flukes in surin province of thailand using geographic information system. | opisthorchiasis, caused by opisthorchis viverrini, is of considerable public health importance in southeast asia, particularly in lao pdr and thailand. | 2015 | 26201130 |
| localization of tubulin from the carcinogenic human liver fluke, opisthorchis viverrini. | opisthorchiasis caused by opisthorchis viverrini, is of considerable public health importance in southeast asia, particularly in lao people's democratic republic and thailand. the infection is associated with a number ofhepatobiliary diseases including cholangitis, obstructive jaundice, hepatomegaly, cholecystitis, cholelithiasis and cholangiocarcinoma. | 2015 | 26201128 |
| genetics of opisthorchis viverrini-related cholangiocarcinoma. | we review the genetic, epigenetic and transcriptional landscape of liver fluke (opisthorchis viverrini, ov)-related cholangiocarcinoma (cca). its distinct alterations, as compared with non-ov-related cca may help shed light on its underlying molecular mechanisms. | 2015 | 25693006 |
| the carcinogenic liver fluke opisthorchis viverrini among rural community people in northeast thailand: a cross- sectional descriptive study using multistage sampling technique. | opisthorchis viverrini infection is a serious public health problem in southeast asia especially in the northeast and north of thailand. therefore, a cross-sectional survey using multistage sampling was conducted from the rural communities of surin province, thailand, during september 2013 to july 2014. o. viverrini infection was determined using kato's thick smear technique. socio-demographic, information resources, and history data were collected using predesigned semi-structured questionnaire ... | 2015 | 26625801 |
| proteomic profile of bithynia siamensis goniomphalos snails upon infection with the carcinogenic liver fluke opisthorchis viverrini. | the snail bithynia siamensis goniomphalos acts as the first intermediate host for the human liver fluke opisthorchis viverrini, the major cause of cholangiocarcinoma (cca) in northeast thailand. the undisputed link between cca and o. viverrini infection has precipitated efforts to understand the molecular basis of host-parasite interactions with a view to ultimately developing new control strategies to combat this carcinogenic infection. to date most effort has focused on the interactions betwee ... | 2015 | 25284051 |
| subtle to severe hepatobiliary morbidity in opisthorchis viverrini endemic settings in southern laos. | evidence of severe hepatobiliary morbidity associated with opisthorchis viverrini liver fluke infection including cholangiocarcinoma (cca) is scarce in laos although o. viverrini infection is highly prevalent. we assessed hepatobiliary morbidity using abdominal ultrasonography (us) in o. viverrini adult patients in saravan province, southern laos. a random sample of 431 o. viverrini patients from 10 villages underwent abdominal us. mild, moderate and markedly advanced periductal fibrosis was dia ... | 2015 | 25275346 |
| carcinogenic liver fluke secretes extracellular vesicles that promote cholangiocytes to adopt a tumorigenic phenotype. | throughout asia, there is an unprecedented link between cholangiocarcinoma and infection with the liver fluke opisthorchis viverrini. multiple processes, including chronic inflammation and secretion of parasite proteins into the biliary epithelium, drive infection toward cancer. until now, the mechanism and effects of parasite protein entry into cholangiocytes was unknown. | 2015 | 25985904 |
| re-examination of opisthorchis viverrini infection in northeast thailand. | liver fluke infection caused by the parasite opisthorchis viverrini (o. viverrini), a human carcinogen, is endemic in north-eastern thailand and remains a major health problem. | 2015 | 25921154 |
| bi-functionality of opisthorchis viverrini aquaporins. | aquaporins (aqp) are essential mediators of water regulation in all living organisms and members of the major intrinsic protein (mip) superfamily of integral membrane proteins. they are potential vehicles or targets for chemotherapy, e.g. in trypanosoma brucei melarsoprol and pentamidine uptake is facilitated by tbaqp-2. transcriptome data suggests that there are at least three active aquaporins in the human liver fluke, opisthorchis viverrini, ovaqp-1, 2 and 3, and crude rna silencing of ovaqp- ... | 2015 | 25461277 |
| advances in the diagnosis of human opisthorchiasis: development of opisthorchis viverrini antigen detection in urine. | many strategies to control opisthorchiasis have been employed in thailand, but not in the other neighbouring countries. specific control methods include mass drug administration (mda) and health education to reduce raw fish consumption. these control efforts have greatly shifted the epidemiology of opisthorchis viverrini (ov) infection over the last decade from presenting as densely concentrated "heavy" infections in single villages to widespread "light" ov infections distributed over wide geogr ... | 2015 | 26485024 |
| patterns of trematode infections of opisthorchis viverrini (opisthorchiidae) and haplorchis taichui (heterophyidae) in human populations from two villages in savannakhet province, lao pdr. | the liver fluke, opisthorchis viverrini, and the minute intestinal fluke, haplorchis taichui, are prevalent in many asian countries. this study analysed the patterns of infections of o. viverrini and h. taichui in lahanam and thakhamlien villages (savannakhet province, lao pdr), in two cross-sectional investigations. out of a total of 207 human participants, post-anthelmintic treatment positivity rates for expelled worms were 170 (82.1%) for h. taichui and 65 (31.4%) for o. viverrini. both these ... | 2015 | 24739959 |
| perceived illness drives participation in mass deworming campaigns in laos. | multiple helminth infections are very common in communities of southern laos. preventive chemotherapy in combination with health education is the mainstay of control. we assessed the knowledge, perceptions and practices of rural communities related to endemic helminthiasis and their control during a mass drug administration (mda) campaign. short interviews with household heads (n=192), direct observations and discussions with attendees of mdas, and in-depth interviews with local opinion leaders ... | 2015 | 24713201 |
| carcinogenic parasite secretes growth factor that accelerates wound healing and potentially promotes neoplasia. | infection with the human liver fluke opisthorchis viverrini induces cancer of the bile ducts, cholangiocarcinoma (cca). injury from feeding activities of this parasite within the human biliary tree causes extensive lesions, wounds that undergo protracted cycles of healing, and re-injury over years of chronic infection. we show that o. viverrini secreted proteins accelerated wound resolution in human cholangiocytes, an outcome that was compromised following silencing of expression of the fluke-de ... | 2015 | 26485648 |
| an ecological study of bithynia snails, the first intermediate host of opisthorchis viverrini in northeast thailand. | infection with the food-borne trematodiasis, liver fluke opisthorchis viverrini, is a major public health concern in southeast asia. while epidemiology and parasitic incidence in humans are well studied, ecological information on the o. viverrini intermediate hosts remains limited. this study aimed to investigate the factors affecting the distribution and abundance of the first intermediate host, bithynia siamensis goniomphalos snails. water quality and snails were sampled in 31 sites in muang d ... | 2015 | 24561073 |
| urinary microrna-192 and microrna-21 as potential indicators for liver fluke-associated cholangiocarcinoma risk group. | opisthorchis viverrini infection induces chronic inflammation in the bile ducts, leading to periductal fibrosis (pdf), which possibly associates to cholangiocarcinoma (cca). patients with cca have a poor prognosis, which is linked to asymptomatic disease and late diagnosis. hence, detecting early stage cca is essential. secretory mirnas have been promoted as biomarkers for pathological changes associated with parasitic infections, fibrosis and/or cancer. we aimed to determine levels of mir-192 a ... | 2015 | 26456596 |
| the carcinogenic liver fluke opisthorchis viverrini is a reservoir for species of helicobacter. | there has been a strong, positive correlation between opisthorchiasis-associated cholangiocarcinoma and infection with helicobacter. here a rodent model of human infection with opisthorchis viverrini was utilized to further investigate relationships of apparent co-infections with o. viverrini and h. pylori. a total of 150 hamsters were assigned to five groups: i) control hamsters not infected with o. viverrini; ii) o. viverrini-infected hamsters; iii) non-o. viverrini infected hamsters treated w ... | 2015 | 25773821 |
| suppression of ov-grn-1 encoding granulin of opisthorchis viverrini inhibits proliferation of biliary epithelial cells. | multistep processes likely underlie cholangiocarcinogenesis induced by chronic infection with the fish-borne liver fluke, opisthorchis viverrini. one process appears to be cellular proliferation of the host bile duct epithelia driven by excretory-secretory (es) products of this pathogen. specifically, the secreted growth factor ov-grn-1, a liver fluke granulin, is a prominent component of es and a known driver of hyper-proliferation of cultured human and mouse cells in vitro. we show potent hype ... | 2015 | 25450776 |