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pleurotus ostreatus inhibits colitis-related colon carcinogenesis in mice.colorectal cancer is one of the leading causes of cancer deaths in both men and women in the world. however, colon cancer can be prevented to some extent by consumption of edible natural products with chemopreventive properties. therefore, we investigated, whether edible mushroom pleurotus ostreatus (po) has chemopreventive effect on inflammation-associated colon carcinogenesis induced by 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (phip) and promoted by dextran sodium sulfate (dss). po trea ...201020878085
medicinal and edible lignicolous fungi as natural sources of antioxidative and antibacterial agents.the antioxidant activity of organic extracts of eight fungal species, ganoderma lucidum, ganoderma applanatum, meripilus giganteus, laetiporus sulphureus, flammulina velutipes, coriolus versicolor, pleurotus ostreatus and panus tigrinus, was evaluated for free radical (dpph· and oh·) scavenging capacity and an effect on lipid peroxidation, and the antibacterial activity was tested by the agar well diffusion method. the highest dpph· scavenging activity was found in the methanol extract of g. app ...201020878697
commonly consumed and specialty dietary mushrooms reduce cellular proliferation in mcf-7 human breast cancer cells.worldwide, over one million women will be newly diagnosed with breast cancer in the next year. moreover, breast cancer is the second leading cause of cancer death in the usa. an accumulating body of evidence suggests that consumption of dietary mushrooms can protect against breast cancer. in this study, we tested and compared the ability of five commonly consumed or specialty mushrooms to modulate cell number balance in the cancer process using mcf-7 human breast cancer cells. hot water extracts ...201020921274
particle and naked rna mycoviruses in industrially cultivated mushroom pleurotus ostreatus in china.many cultivated mushroom strains, such as pleurotus ostreatus td300, displayed symptoms of degeneration. a spherical virus posv and four dsrna segments were extracted from mycelium of p. ostreatus td300. posv had a diameter of 23 nm and encapsidated a 2.5kb dsrna segment with coat proteins whose molecular weights were 39 kda and 30 kda. four dsrna segments were 8.2 kb, 2.5 kb, 2.0 kb, and 1.1 kb in size, respectively. the 1.1 kb dsrna segment often escaped detection. the cdna and the amino acid ...201020943162
assessment of heavy metals in some wild edible mushrooms collected from yunnan province, china.eight heavy metals (cu, zn, fe, mn, cd, cr, ni, and pb) in 14 different wild-growing edible mushroom species (coprinus comatus, voluariella volvacea, pleurotus nebrodensis, hypsizigus marmoreus, hericium erinaceus, agrocybe aegerita, lenfinus edodes, collybia velutipes, agaricus bisporus, russula albida, clitocybe conglobata, pleurotus eryngii, lepista sordida, and pleurotus ostreatus) collected from yunnan province, china, were determined by inductively coupled plasma-atomic emission spectromet ...201020976551
extraction and application of laccases from shimeji mushrooms (pleurotus ostreatus) residues in decolourisation of reactive dyes and a comparative study using commercial laccase from aspergillus oryzae.oxidases are able to degrade organic pollutants; however, high costs associated with biocatalysts production still hinder their use in environmental biocatalysis. our study compared the action of a commercial laccase from aspergillus oryzae and a rich extract from pleurotus ostreatus cultivation residues in decolourisation of reactive dyes: drimaren blue x-3lr (dmblr), drimaren blue x-bln (dmbbln), drimaren rubinol x-3lr (dmr), and drimaren blue c-r (rbbr). the colour removal was evaluated by co ...201021052547
in vitro antitumor and immunomodulatory effects of the protein pcp-3a from mushroom pleurotus citrinopileatus.a nonlectin glycoprotein (pcp-3a) newly isolated from the fruit body of edible golden oyster mushroom pleurotus citrinopileatus has been shown to be growth inhibitory against human myeloid leukemic u937 cells in a previous report. there is a well-recognized relation between antitumor activity and immunomodulation. the immunomodulatory activity of pcp-3a was therefore assessed in the present study. human mononuclear cells (mnc) and the cd4(+) t lymphocytes isolated from them were stimulated separ ...201021053905
in vivo bioavailability of selenium in enriched pleurotus ostreatus mushrooms.the in vivo bioavailability of se was investigated in enriched pleurotus ostreatus mushrooms. a bioavailability study was performed using 64 wistar male rats separated in 8 groups and fed with different diets: without se, with mushrooms without se, with enriched mushrooms containing 0.15, 0.30 or 0.45 mg kg(-1) se and a normal diet containing 0.15 mg kg(-1) of se using sodium selenate. the experiment was performed in two periods: depletion (14 days) and repletion (21 days), according to the asso ...201021069148
production of laccases by pleurotus ostreatus in submerged fermentation in co-culture with trichoderma viride.to evaluate the production and stability of laccases by pleurotus ostreatus in liquid co-cultures with trichoderma viride as a function of infection time and agitation rate.201019709340
bleach enhancement of mixed wood pulp by xylanase-laccase concoction derived through co-culture strategy.mixed enzyme preparation having both xylanase and laccase activity was evaluated for its bleach enhancing ability of mixed wood pulp. the enzyme was produced through co-cultivation of mutant penicillium oxalicum sau(e)-3.510 and pleurotus ostreatus mtcc 1804 under solid-state fermentation. bleaching of pulp with mixed enzyme had resulted into a notable decrease in kappa number and increased brightness as compared to xylanase alone. analysis of bleaching conditions had denoted that 8 iu g(-1) of ...201019421722
potentiation of innate immunity by β-glucans.β-glucans have been known to exhibit antitumor activities by potentiating host immunity by an unknown mechanism. the c-type lectin dectin-1, a β-glucan receptor, is found on the macrophage and can recognize various β-glucans. previously, we demonstrated the presence of β-glucan receptor, dectin-1, on the raw 264.7 cells as well as on murine mucosal organs, such as the thymus, the lung, and the spleen. in order to investigate immunopotentiation of innate immunity by β-glucan, we stimulated a muri ...201023956643
variations of lignocellulosic activities in dual cultures of pleurotus ostreatus and trichoderma longibrachiatum on unsterilized wheat straw.trichoderma spp., soil filamentous fungi, are antagonists that can cause great losses in mushroom production. we have investigated the influence of t. longibrachiatum on the production of lignocellulolytic enzymes by pleurotus ostreatus during its vegetative growth on a straw-based cultivation substrate that either had been sterilized, pasteurized or not heat treated. the variations in the lignocellulolytic activities and the electrophoretic patterns in single and dual cultures were used as a to ...201021148892
electrostatically self-assembled azides on zinc sulfide nanoparticles as multifunctional nanoprobes for peptide and protein analysis in maldi-tof ms.a simple method to synthesize electrostatically self-assembled azides on zinc sulfide nanoparticles (zns-n(3) nps) was described and then it was further applied as a multifunctional nanoprobe such as enriching, desalting, accelerating and separation-/washing free nanoprobes for rapid analysis of peptides and proteins and microwave assisted tryptic digested proteins in matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (maldi-tof ms). the zns-n(3) nps were characterized ...201020602933
development of an efficient and simple method for conjugation of laccase to immunoglobulin and its characterization by enzyme immunoassay.a new laccase-conjugated antibody is developed, containing laccase as an enzyme marker, obtained from culture supernatant of the basidiomycetous fungi pleurotus ostreatus. the efficacy of the laccase-conjugated antibody was demonstrated in indirect and direct enzyme immunoassay after using periodate and glutaraldehyde conjugation methods. the assay based on laccase-conjugated antibody is potent to detect antigens when compared with peroxidase conjugated antibody.201020623408
α-(1 → 3)-d-glucans from fruiting bodies of selected macromycetes fungi and the biological activity of their carboxymethylated products.purpose of work: to show biological activity of carboxymethylated α-(1 → 3)-d-glucans isolated from the selected macromycetes fungi on human tumor and normal cells. water-insoluble, alkali-soluble polysaccharides (wip) were isolated from fruiting bodies of four macromycetes fungi: lentinus edodes, pleurotus ostreatus, piptoporus betulinus and laetiporus sulphureus. the structure of the polysaccharides was determined using composition analysis, methylation analysis, fourier transform infrared spe ...201021188616
effect of different substrates and casing materials on the growth and yield of calocybe indica.calocybe indica, a tropical edible mushroom, is popular because it has good nutritive value and it can be cultivated commercially. the current investigation was undertaken to determine a suitable substrate and the appropriate thickness of casing materials for the cultivation of c. indica. optimum mycelial growth was observed in coconut coir substrate. primordia initiation with the different substrates and casing materials was observed between the 13th and 19th day. the maximum length of stalk wa ...201023956634
isolation and characterization of a novel ribonuclease from the pink oyster mushroom pleurotus djamor.a 15-kda rnase was purified from pleurotus djamor using ion exchange chromatography and gel filtration. its n-terminal amino acid sequence was different from previously reported rnase sequences of mushrooms belonging to the genus of pleurotus and other genera. the rnase exhibited maximal rnase activity at ph 4.6 and 60 degrees c. its activity toward polyhomoribonucleotides was poly(u) > poly(c) > poly(a) > poly(g). it inhibited proliferation of hepatoma cells and breast cancer cells. the ranking ...201020647680
yield performances and changes in enzyme activities of pleurotus spp. (p. ostreatus and p. sajor-caju) cultivated on different agricultural wastes.pleurotus ostreatus and pleurotus sajor-caju were studied for their ability to produce laccase and carboxymethylcellulase (cmcase) enzymes on different agricultural wastes under solid state fermentation. the spawns of p. ostreatus and p. sajor-caju were inoculated on different agricultural wastes including viticulture wastes, wheat straw, paddy straw, sesame straw, sawdust as well as the mixtures of these wastes with wheat bran. the carbon and nitrogen contents of substrates containing bran were ...201020056410
search for new naturally occurring strains of pleurotus to improve yields. pleurotus albidus as a novel proposed species for mushroom production.background: the species of genus pleurotus are worldwide cultivated. aims: to evaluate growth, yield production and morphological variations of fruiting bodies obtained from the cultivation of fourteen naturally occurring pleurotus strains isolated from argentina. methods: the strains growth was tested at different temperatures on nobles' medium. substrates assayed were: supplemented salix sawdust, supplemented and non supplemented wheat straw. the species studied were pleurotus albidus, pleurot ...201121241817
morphology and laccase production of white-rot fungi grown on wheat bran flakes under semi-solid-state fermentation conditions.in this paper, we studied the laccase production and the growth morphology of different white-rot fungi, i.e. pleurotus ostreatus, trametes pubescens, cerrena unicolor and trametes versicolor, cultured under semi-solid-state fermentation conditions using wheat bran flakes as a natural low-cost support substrate. trametes versicolor exhibited the highest laccase activity per gram of total dry matter, followed by p. ostreatus (63.5 and 58.2ug(-1) , respectively). in addition, they showed a time pr ...201121291496
disposable diapers biodegradation by the fungus pleurotus ostreatus.this research assesses the feasibility of degrading used disposable diapers, an important component (5-15% in weight) of urban solid waste in mexico, by the activity of the fungus pleurotus ostreatus, also known as oyster mushroom. disposable diapers contain polyethylene, polypropylene and a super absorbent polymer. nevertheless, its main component is cellulose, which degrades slowly. p. ostreatus has been utilized extensively to degrade cellulosic materials of agroindustrial sources, using in s ...201121474296
skin test reactivity of allergic subjects to basidiomycetes' crude extracts in a tropical environment.fungal allergies can be detected by the skin prick test with extracts of the organisms, but not all fungi, including the basidiomycetes, are being examined. we determined the level of sensitization to basidiomycetes in allergic subjects and compared their reactivity to commercial extracts commonly used to detect allergies. crude spore extracts of the basidiomycetes ganoderma applanatum, chlorophyllum molybdites, and pleurotus ostreatus, which are known to release numerous spores, were examined a ...201121506892
anti-inflammatory activity of edible oyster mushroom is mediated through the inhibition of nf-kb and ap-1 signaling.abstract: background: mushrooms are well recognized for their culinary properties as well as for their potency to enhance immune response. in the present study, we evaluated anti-inflammatory properties of an edible oyster mushroom (pleurotus ostreatus) in vitro and in vivo. methods: raw264.7 murine macrophage cell line and murine splenocytes were incubated with the oyster mushroom concentrate (omc, 0-100 ug/ml) in the absence or presence of lipopolysacharide (lps) or concanavalin a (cona), resp ...201121575254
inhibitory effect of a glycoprotein isolated from golden oyster mushroom (pleurotus citrinopileatus) on the lipopolysaccharide-induced inflammatory reaction in raw 264.7 macrophage.mushrooms have become an important source of natural antitumor, antiviral, anti-bacterial, immunomodulatory, and anti-inflammatioy agents. golden oyster mushroom, pleurotus citrinopileatus, is a common mushroom in oriental countries for human consumption. the present study was to investigate the anti-inflammatory reaction and immunomodulatory of the bioactive non-lectin glycoprotein (pcp-3a) isolated from the fresh fruiting body of this mushroom. western blot analysis on lps-induced inos, cox-2, ...201121627087
co-cultivation of mutant penicillium oxalicum sau(e)-3.510 and pleurotus ostreatus for simultaneous biosynthesis of xylanase and laccase under solid-state fermentation.co-cultivation of mutant penicillium oxalicum sau(e)-3.510 and pleurotus ostreatus mtcc 1804 was evaluated for the production of xylanase-laccase mixture under solid-state fermentation (ssf) condition. growth compatibility between mutant p. oxalicum sau(e)-3.510 and white rot fungi (p. ostreatus mtcc 1804, trametes hirsuta mtcc 136 and pycnoporus sp. mtcc 137) was analyzed by growing them on potato dextrose agar plate. extracellular enzyme activities were determined spectrophotometrically. under ...201121642024
co-cultured production of lignin-modifying enzymes with white-rot fungi.co-cultivation was a potential strategy in lignocellulolytic biodegradation with producing high activity enzymes due to their synergistic action. the objective of this study was to investigate the rarely understood effects of co-culturing of two white-rot fungi on lignin-modifying enzymes (lmes) production. six species, bjerkandera adusta, phlebia radiata, pleurotus ostreatus, dichomitus squalens, hypoxylon fragiforme and pleurotus eryngii, were cultured in pairs to study the production of lmes. ...201121647688
fungal solid state fermentation on agro-industrial wastes for acid wastewater decolorization in a continuous flow packed-bed bioreactor.this study was aimed at developing a process of solid state fermentation (ssf) with the fungi pleurotus ostreatus and trametes versicolor on apple processing residues for wastewater decolorization. both fungi were able to colonize apple residues without any addition of nutrients, material support or water. p. ostreatus produced the highest levels of laccases (up to 9u g(-1) of dry matter) and xylanases (up to 80u g(-1) of dry matter). a repeated batch decolorization experiment was set up with ap ...201121652205
response to oyster mushroom (pleurotus ostreatus) extract by sensitized and nonsensitized guinea pig trachea.occupational exposure to oyster mushroom (pleurotus ostreatus) has been associated with obstructive lung disease. previously, we studied an extract of oyster mushroom (ome) and determined that it causes dose-dependent contractions of nonsensitized guinea pig trachea (gpt). we extend these studies to the investigation of sensitized tissue. in the present study 24 animals were sensitized using ovalbumin (oa) and subsequently challenged with an aerosol of 2.5% oa. a control group of 12 nonsensitize ...201121667260
anti-cancer effects of protein extracts from calvatia lilacina, pleurotus ostreatus and volvariella volvacea.calvatia lilacina (cl), pleurotus ostreatus (po) and volvariella volvacea (vv) are widely distributed worldwide and commonly eaten as mushrooms. in this study, cell viabilities were evaluated for a human colorectal adenocarcinoma cell line (sw480 cells) and a human monocytic leukemia cell line (thp-1 cells). apoptotic mechanisms induced by the protein extracts of po and vv were evaluated for sw480 cells. the viabilities of thp-1 and sw480 cells decreased in a concentration-dependent manner after ...201121792367
antihyperlipidemic effects of pleurotus ostreatus (oyster mushrooms) in hiv-infected individuals taking antiretroviral therapy.abstract:201121831304
comparative study of antioxidant activities and total phenolic content of selected edible wild mushrooms.the present study aims to assess the antioxidant activities (aoa) and total phenolic content (tpc) of water extracts of selected edible wild mushrooms: pleurotus porrigens, schizophyllum commune, hygrocybe conica, and lentinus ciliatus. the aoa were evaluated against dpph radical and abts radical cation scavenging ability, ferric-reducing antioxidant power (frap) and beta-carotene-linoleate bleaching (beta-cb) assays, and the folin-ciocalteu method for tpc. bha was used as reference. p. porrigen ...201122135876
Engineered tobacco and microalgae secreting the fungal laccase POXA1b reduce phenol content in olive oil mill wastewater.Olive oil mill wastewaters (OMWs) are characterised by low pH and a high content of mono- and polyaromatic compounds that exert microbial and phytotoxic activity. The laccase cDNA of the poxA1b gene from Pleurotus ostreatus, carrying a signal peptide sequence for enzyme secretion and driven by the CaMV 35S promoter, was cloned into a plant expression vector. Nuclear genetic transformation was carried out by co-cultivation of Agrobacterium tumefaciens with tobacco cv Samsun NN leaves and cells of ...201122142729
a family gh51 α-l: -arabinofuranosidase from pleurotus ostreatus: identification, recombinant expression and characterization.an α-l: -arabinofuranosidase produced by pleurotus ostreatus (poabf) during solid state fermentation on tomato pomace was identified and the corresponding gene and cdna were cloned and sequenced. molecular analysis showed that the poabf gene carries 26 exons interrupted by 25 introns and has an open reading frame encoding a protein of 646 amino acid residues, including a signal peptide of 20 amino acid residues. the amino acid sequence similar to the other α-l: -arabinofuranosidases indicated th ...201122080345
Acylhomoserine lactone production by bacteria associated with cultivated mushrooms.The main bacterial pathogens of cultivated mushroom as well as mushroom-associated bacteria, which were isolated from Agaricus bisporus, Pleurotus ostreatus and Pleurotus eryngii mushroom niches, were evaluated for the production of N-acyl-L-homoserine lactones (AHLs) by using four bioreporters. Furthermore, identification of AHLs by LC-ESI-FTICR MS was performed on culture filtrates of selected pathogens and mushroom-associated bacteria strains, which resulted in inducing at least one of the fo ...201121942309
Antioxidant properties of extracts of wild medicinal mushroom species from Croatia.Antioxidant activity and total phenol (TP) content of methanol and water extracts of three wild Croatian mushroom species Auricularia auricula-judae (Bull.) Quél., Sarcoscypha austriaca (Sacc.) Boud., and Strobilurus esculentus (Wulfen) Singer were determined and compared with the values obtained for extracts of four cultivated mushrooms Agaricus bisporus (J.E. Lange) Imbach (brown and white strains), Pleurotus ostreatus (Jacq.) P. Kumm., and Lentinus edodes (Berk.) Singer. Spectrophotometric de ...201122135877
chemical composition and nutrition value of dried cultivated culinary-medicinal mushrooms from turkey.dietary fiber, raw fiber, fructose, glucose, sucrose, maltose, vitamin a (retinol), b1 (thiamine), b2 (riboflavin), b6 (pyridoxine), and niacin contents of dried cultivated mushroom species agaricus bisporus (white and brown), lentinus edodes, and pleurotus ostreatus were determined and evaluated for nutrient and chemical composition. assays of dried mushroom samples were carried out after the drying process. dried shiitake samples showed the highest dietary fiber and raw fiber content (23.23 +/ ...201122164765
biodegradation of chlorobenzoic acids by ligninolytic fungi.we investigated the abilities of several perspective ligninolytic fungal strains to degrade 12 mono-, di- and trichloro representatives of chlorobenzoic acids (cbas) under model liquid conditions and in contaminated soil. attention was also paid to toxicity changes during the degradation, estimated using two luminescent assay variations with vibrio fischeri. the results show that almost all the fungi were able to efficiently degrade cbas in liquid media, where irpex lacteus, pycnoporus cinnabari ...201121963171
in vitro prevention of cataract by oyster mushroom pleurotus florida extract on isolated goat eye lens.the aim of the present work was to evaluate the in vitro effect of pleurotus florida extract cataract induced by glucose.201122144771
Chemical composition and nutritional value of the most widely appreciated cultivated mushrooms: An inter-species comparative study.Herein, it was reported and compared the chemical composition and nutritional value of the most consumed species as fresh cultivated mushrooms: Agaricus bisporus (white and brown mushrooms), Pleurotus ostreatus (oyster mushroom), Pleurotus eryngii (King oyster mushroom), Lentinula edodes (Shiitake) and Flammulina velutipes (Golden needle mushroom). Shiitake revealed the highest levels of macronutrients, unless proteins, as also the highest sugars, tocopherols and PUFA levels, and the lowest SFA ...201122056333
Profiling of hepatic gene expression of mice fed with edible japanese mushrooms by DNA microarray analysis: comparison among Pleurotus ostreatus, Grifola frondosa, and Hypsizigus marmoreus.To compare and estimate the effects of dietary intake of three kinds of mushrooms (Pleurotus ostreatus, Grifola frondosa, and Hypsizigus marmoreus), mice were fed a diet containing 10-14% of each mushroom for 4 weeks. Triacylglycerol in the liver and plasma decreased and plasma cholesterol increased in the P. ostreatus-fed group compared with those in the control group. Cholesterol in the liver was lower in the G. frondosa-fed group than in the control group, but no changes were found in the H. ...201121910414
Trypanocidal Activity of Ergosterol Peroxide from Pleurotus ostreatus.Chagas' disease, which is caused by the protozoan parasite Trypanosoma cruzi, is a public health problem in South America affecting millions of people, and more recently several thousands in countries where the disease is not endemic. Due to the magnitude of the problem, finding a cure for this disease remains a major challenge. The aim of this study is to evaluate the trypanocidal activity of ergosterol peroxide (5a, 8a-epidioxy-22E-ergosta-6, 22-dien-3ß-ol) isolated from Pleurotus ostreatus (J ...201122083593
Thermus composti sp. nov., isolated from oyster mushroom compost.A Gram-negative, rod-shaped bacterium strain (K-39(T)) was isolated from the thermophilic phase of the composting process for oyster mushroom substrate preparation. The strain grew at 40-80 °C (optimum, 65-75 °C), at pH 5-9 (optimum, pH 7), in media containing up to 1.5% (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain K-39(T) formed a distinct lineage within the genus Thermus. Its closest cultivated relative was Thermus islandicus PRI 3838(T) (96.8% similari ...201121856987
indole compounds in some culinary-medicinal higher basidiomycetes from poland.methanolic extracts of two species collected from natural habitats in poland, boletus edulis and suillus luteus, and one species from a commercial source, pleurotus ostreatus, were analyzed for the presence of non-hallucinogenic indole compounds. the contents of indole compounds in these species were both qualitatively and quantitatively diverse, ranging from 0.01 to 34.11 mg/100 g d.w. two of 11 tested indole compounds, 5-hydroxytryptophan (0.18, 2.08, 1.63 mg/100 g d.w.) and serotonin (6.52, 1 ...201122324410
antitumor effect of culinary-medicinal oyster mushroom, pleurotus ostreatus (jacq.: fr.) p. kumm., derived protein fraction on tumor-bearing mice models.previously, we reported the in vitro anticancer and immunomodulatory effect of a protein fraction designated as cibacron blue affinity purified protein (cbaep) obtained from the culinary-medicinal oyster mushroom, pleurotus ostreatus. in the present study, we investigated the in vivo antitumor potential of cbaep in different tumor-bearing mice models and studied the detailed mechanism of tumor regression in dalton lymphoma (dl)-bearing mice. the lethal dose (ld50) of cbaep was found to be 55 mg/ ...201122324408
optimization and kinetics evaluation of biodegradation of synthetic azo reactive dye by fungal consortium.wastewater containing direct dyes discharged from various industries, in particular, textile industry, often cause many environmental problems. among the various effluent treatment methods, biological methods found to be cost effective and do not end up in secondary pollutants. in this study, an attempt has been made to study the decolorization of cibacron yellow s-3r, an azo reactive dye by using fungal cultures such as coriolus versicolor, phanerochaete chrysosporium, pleurotus ostreatus, and ...201123505830
characterization of pseudomonads isolated from decaying sporocarps of oyster mushroom.pleurotus ostreatus is one of the most extensively cultivated mushrooms in the world; however, the success of cultivation often depends on the proliferation of different bacterial pathogens. pseudomonas tolaasii is thought as the major cause of brown blotch disease of agaricus bisporus and yellowing of pleurotus ostreatus. in this study we examined the pathogenicity and assessed the industrial damage causing effect of 41 pseudomonas strains isolated from deformed, yellowing oyster mushroom (p. o ...201120627228
potential for bioremediation of agro-industrial effluents with high loads of pesticides by selected fungi.wastewaters from the fruit packaging industry contain a high pesticide load and require treatment before their environmental discharge. we provide first evidence for the potential bioremediation of these wastewaters. three white rot fungi (wrf) (phanerochaete chrysosporium, trametes versicolor, pleurotus ostreatus) and an aspergillus niger strain were tested in straw extract medium (stem) and soil extract medium (sem) for degrading the pesticides thiabendazole (tbz), imazalil (imz), thiophanate ...201120635121
biological removal of azo and triphenylmethane dyes and toxicity of process by-products.increasing environmental pollution is connected with broad applications of dyes and imperfection of dyeing technology. decolourization of triphenylmethane brilliant green and disazo evans blue by bacterial and fungal strains and toxicity (phyto- and zootoxicity) of degradation by-products were investigated. influence of incubation method on dyes removal was evaluated (static, semi-static, shaken). dead biomass was used for sorption estimation. toxicity of treated dyes was measured to estimate po ...201122593606
photo-irradiated biosynthesis of silver nanoparticles using edible mushroom pleurotus florida and their antibacterial activity studies.this is a report on photo-irradiated extracellular synthesis of silver nanoparticles using the aqueous extract of edible oyster mushroom (pleurotus florida) as a reducing agent. the appearance, size, and shape of the silver nanoparticles are understood by uv-visible spectroscopy, field emission scanning electron microscopy, transmission electron microscopy, and atomic force microscopy. the x-ray diffraction studies, energy dispersive x-ray analysis indicate that particles are crystalline in natu ...201122190895
the effects of β-glucan isolated from pleurotus ostreatus on methotrexate treatment in rats with adjuvant arthritis.the purpose of this study was to evaluate the effect of β-(1,3/1,6)-d: -glucan isolated from pleurotus ostreatus (β-glucan-po) on prophylactic treatment of adjuvant arthritis (aa) with methotrexate (mtx) in rats. groups of rats with aa were treated with methotrexate (1 mg/kg/week), β-glucan-po (1 mg/kg every second day) or their combination for the period of 28 days from adjuvant application. body mass, hind paw swelling, arthrogram scores and a level of serum albumin were measured as markers of ...201120033413
enzyme production by solid substrate fermentation of pleurotus ostreatus and trametes versicolor on tomato pomace.a process of solid state fermentation (ssf) on tomato pomace was developed with the white-rot fungi pleurotus ostreatus and trametes versicolor, using sorghum stalks as support. operative parameters (humidity, water activity, and size of substrate particles) guaranteeing a good colonization of tomato pomace by both fungi were defined and conditions for production at high titers of the industrially relevant enzymes laccase, xylanase and protease were identified. significant laccase activity level ...201120582639
a heteropolysaccharide from aqueous extract of an edible mushroom, pleurotus ostreatus cultivar: structural and biological studies.a water soluble polysaccharide isolated from the hot aqueous extract of pleurotusostreatus cultivar was found to contain d-glucose and d-galactose in a molar ratio of nearly 7:1. structural investigation was carried out using acid hydrolysis, methylation analysis, periodate oxidation, smith degradation, and nmr studies ((1)h, (13)c, dept-135, tocsy, dqf-cosy, noesy, roesy, hmqc, and hmbc). on the basis of the above mentioned experiments the structure of the repeating unit of the polysaccharide w ...201121129732
pleuran (β-glucan from pleurotus ostreatus) supplementation, cellular immune response and respiratory tract infections in athletes.prolonged and exhausting physical activity causes numerous changes in immunity and sometimes transient increases the risk of upper respiratory tract infections (urtis). nutritional supplements as countermeasures to exercise-induced changes have increasingly been studied in the last decade. one of the most promising nutritional supplements is β-glucan, a well-known immunomodulator with positive effects on the function of immunocompetent cells. in this double blind, placebo-controlled study, we in ...201121249381
pleurotus ostreatus spent mushroom compost as green biosorbent for nickel (ii) biosorption.the potential of pleurotus ostreatus spent mushroom compost (psmc) as a green biosorbent for nickel (ii) biosorption was investigated in this study. a novel approach of using the half-saturation concentration of biosorbent to rapidly determine the uptake, kinetics and mechanism of biosorption was employed together with cost per unit uptake analysis to determine the potential of this biosorbent. fifty per cent nickel (ii) biosorption was obtained at a half-saturation constant of 0.7 g biosorbent ...201122170837
cultivation of the culinary-medicinal lung oyster mushroom, pleurotus pulmonarius (fr.) quél. (agaricomycetideae) on grass plants in taiwan.cultivation of the culinary-medicinal lung oyster mushroom, pleurotus pulmonarius, on the stalks of three grass plants, i.e., panicum repens, pennisetum purpureum, and zea mays were investigated. the effects of various combinatorial substrates on mushroom mycelial growth and yield calculated as biological efficiency (be) were determined. among 9 experimental substrates, the most suitable substrate for mycelial growth was 45zms:45s, followed by 45prs:45s; their mycelial growth rates were obviousl ...201122135896
pleurotus ostreatus heme peroxidases: an in silico analysis from the genome sequence to the enzyme molecular structure.an exhaustive screening of the pleurotus ostreatus genome was performed to search for nucleotide sequences of heme peroxidases in this white-rot fungus, which could be useful for different biotechnological applications. after sequence identification and manual curation of the corresponding genes and cdnas, the deduced amino acid sequences were converted into structural homology models. a comparative study of these sequences and their structural models with those of known fungal peroxidases revea ...201122078736
fungal laccases: versatile tools for lignocellulose transformation.conversion of lignocellulosic materials to useful, high value products normally requires a pre-treatment step to transform or deconstruct the recalcitrant and heterogeneous lignin fraction. the development of "green tools" for the transformation of lignocellulosic feedstocks is in high demand for a sustainable exploitation of such resources. this multi-faceted challenge is being addressed by an ever-increasing suite of ligninolytic enzymes isolated from various sources. among these, fungal lacca ...201122078735
low impact strategies to improve ligninolytic enzyme production in filamentous fungi: the case of laccase in pleurotus ostreatus.the ever-increasing demand of laccases for biodelignification, industrial oxidative processes and environmental bioremediation requires the production of large quantities of enzymes at low cost. the present work was carried out to reduce laccase production costs in liquid fermentations of the white-rot fungus pleurotus ostreatus through two different approaches. in the first, screening of fungal spent media as natural laccase inducer was performed, eliminating the presence of potentially toxic/r ...201122078734
genomics and transcriptomics characterization of genes expressed during postharvest at 4°c by the edible basidiomycete pleurotus ostreatus.pleurotus ostreatus is an industrially cultivated basidiomycete with nutritional and environmental applications. its genome, which was sequenced by the joint genome institute, has become a model for lignin degradation and for fungal genomics and transcriptomics studies. the complete p. ostreatus genome contains 35 mbp organized in 11 chromosomes, and two different haploid genomes have been individually sequenced. in this work, genomics and transcriptomics approaches were employed in the study of ...201122069155
biosorption of methylene blue onto spent corncob substrate: kinetics, equilibrium and thermodynamic studies.this study focuses on the possible use of the spent corncob substrate (scs), an agricultural waste used after the cultivation of white rot fungus pleurotus ostreatus, to adsorb methylene blue (mb) from aqueous solutions. a batch adsorption study was carried out with variable solution ph, adsorption time, temperature and initial mb concentration. it was found that mb uptake was favorable at ph ranging from 4.0 to 12.0 and the equilibrium adsorption capacity can be reached promptly within about 18 ...201122049698
influence of pleurotus ostreatus inoculation on wood degradation and fungal colonization.the influence of pleurotus ostreatus inoculation on wood degradation and on fungal community structure was studied. the experiments were performed on an organically poor fly ash deposit covered with a 10 cm layer of beech wood chips inoculated with p. ostreatus isolate zim76. compared to non-inoculated wood chips, inoculation increased the temperatures and relative humidities and, in the first 6 months, accelerated klason lignin degradation by 9% and also, after 17 months, increased iron translo ...201121963903
influence of calcium and silicon supplementation into pleurotus ostreatus substrates on quality of fresh and canned mushrooms.supplements of gypsum (calcium source), pumice (silicon source) and pumice sulfate (silicon and calcium source) into substrates for oyster mushrooms (pleurotus ostreatus) were searched for their effects on production as well as qualities of fresh and canned mushrooms. the addition of pumice up to 30% had no effect on total yield, size distribution and cap diameters. the supplementation of gypsum at 10% decreased the total yield; and although gypsum at 5% did not affect total yield, the treatment ...201121917642
transformation of the recalcitrant pharmaceutical compound carbamazepine by pleurotus ostreatus: role of cytochrome p450 monooxygenase and manganese peroxidase.carbamazepine (cbz) is an environmentally recalcitrant compound highly stable in soil and during wastewater treatment. in this study, we examined the mechanisms by which the white-rot fungus pleurotus ostreatus metabolizes cbz in liquid culture using a physiological approach. p. ostreatus pc9 was grown in media known to support different levels of a multiplicity of enzyme systems such as cytochrome p450 (cyp450) and manganese peroxidase (mnp). when both cyp450 and mnp systems were active, 99% of ...201121744850
the use of a white rot fungi (pleurotus ostreatus) immobilized on amberlite xad-4 as a new biosorbent in trace metal determination.the present work proposes the use of pleurotus ostreatus immobilized on amberlite xad-4 as new biosorbent in trace metal determination. the effects of experimental parameters, such as "ph and flow rate of sample solution, amount of solid phase, eluent type, and concentration" on the recovery of the metal ions were investigated. maximum adsorption of cr(iii), cd(ii) and cu(ii) ions took place in the ph range 4-5. these metal ions can be desorbed with 1m hcl (recovery 95-100%). 0.2g adsorbent amou ...201121737258
agaricus blazei production on non-composted substrates based on sunflower seed hulls and spent oyster mushroom substrate.agaricus blazei murrill is usually cultivated using the same biphasic composting method employed for a. bisporus. because cultivation of a. blazei on traditional a. bisporus composts poses some disadvantages, non-composted substrates were studied for a. blazei cultivation. mycelial growth rate and productive performance of a. blazei were evaluated on substrates containing sunflower seed hulls, pleurotus spp. spent mushroom substrate, or their combination, in the absence or in the presence of dif ...201125187132
effect of light and reductones on differentiation of pleurotus ostreatus.vegetative mycelia of pleurotus ostreatus were differentiated into primordia and subsequently into fruit bodies in synthetic sucrose-asparagine medium when exposed to light at low temperature. during photo-morphogenesis, l-ascorbic acid-like substances called reductones were produced. l-ascorbic acid, d-erythroascorbic acid, 5-o-(α-d-glucopyranosyl)-d-erythroascorbic acid, 5-o-(α-d-xylopyranosyl)-d-erythroascorbic acid, 5-methyl-5-o-(α-d-glucopyranosyl)-d-erythroascorbic acid and 5-methyl-5-o-(α ...201121369982
hypolipidemic activities of dietary pleurotus ostreatus in hypercholesterolemic rats.this work was conducted to investigate dietary supplementation of oyster mushroom fruiting bodies on biochemical and histological changes in hyper and normocholesterolemic rats. six-week old female sprague-dawley albino rats were divided into three groups of 10 rats each. feeding a diet containing a 5% powder of pleurotus ostreatus fruiting bodies to hypercholesterolemic rats reduced plasma total cholesterol, triglyceride, low-density lipoprotein (ldl), total lipid, phospholipids, and ldl/high-d ...201122783072
application of 2iii7-3 fractional factorial experimental design to enhance enzymatic activities of pleurotus ostreatus with high concentrations of polychlorinated biphenyls.a 2(iii)(7-3) fractional factorial experimental design was used to establish 16 culture media, with and without pcbs to enhance the activities of laccase (lac), manganese peroxidase (mnp), and versatile peroxidase (vp) produced by the white rot fungus pleurotus ostreatus. the culture was added to 10,000 mg l(-1) of transformer oil, containing 71% of the identified arochlor 1242. the culture conditions were established with eight variables at two values (levels); ph (4 and 6), agitation (100 and ...201121308601
dietary effect of pleurotus eryngii on biochemical function and histology in hypercholesterolemic rats.this work was conducted to investigate diet supplement of king oyster mushroom fruiting bodies on biochemical and histological changes in hypercholesterolemic rats. six-week old female sprague-dawley albino rats were divided into three groups of 10 rats each. the feeding of 5% powder of the fruiting bodies of pleurotus eryngii to hypercholesterolemic rats reduced their plasma total cholesterol, triglyceride, low-density lipoprotein, total lipid, phospholipids, and ldl/hdl ratio by 24.05%, 46.33% ...201123961153
effect of calcinated oyster shell powder on growth, yield, spawn run, and primordial formation of king oyster mushroom (pleurotus eryngii).this study was conducted to evaluate the calcium (ca) absorption efficacy of king oyster mushroom (pleurotus eryngii) grown on sawdust medium supplemented with ca-sources, including oyster shell powder, and to determine the efficacy of oyster shell powder as a calcium supplement on growth, yield, spawn run and primordial formation of p. eryngii. optimum calcination of oyster shell powder was achieved at the temperature of 620.56 °c. a 1% supplementation of oyster shell powder in sawdust medium d ...201121394076
removal of neutral red from aqueous solution by adsorption on spent cottonseed hull substrate.cottonseed hull, a low-cost widely available agricultural waste in china, after used as substrate for the white rot fungus pleurotus ostreatus cultivation, was tested for the removal of neutral red (nr), a cationic dye, from aqueous solution. a batch adsorption study was carried out with varied solution ph, adsorbent dosage, reaction time and initial nr concentration. the results show that the kinetics of dye removal by the spent cottonseed hull substrate (schs) is prompt in the first 5 min and ...201120933326
induction and purification by three-phase partitioning of aryl alcohol oxidase (aao) from pleurotus ostreatus.aryl alcohol oxidase (aao) is an extracellular flavoenzyme involved in lignin degradation by white rot fungi. screening of lignolytic and aao activity from twenty different fungal species were carried out. among them, seven species showed lignolytic activity and three of them (pleurotus ostreatus, pleurotus eous, and pleurotus platypus) were found to be aao positive. maximal aao activity was observed in batch cultures of p. ostreatus and was found to be induced by aromatic amino acids and aryl a ...201120857231
particle geometry affects differentially substrate composition and enzyme profiles by pleurotus ostreatus growing on sugar cane bagasse.the growth of pleurotus ostreatus was analyzed on three particle sizes of sugar cane bagasse: 0.92 mm and 1.68 mm in diameter, in addition to heterogeneous fibers (average 2.9 mm in diameter). specific growth rate on heterogeneous particles was lower (μ=0.043 h(-1)), although soluble protein production was maximal (809 μg/g dry wt). higher μ values were reached on the other two particles sizes (0.049-0.05 h(-1)) with less soluble protein (500 μg/g dry wt). xylanases and laccases were favored in ...201120846859
increasing pleurotus ostreatus laccase production by culture medium optimization and copper/lignin synergistic induction.laccases have great biotechnological potential in diverse industries as they catalyze the oxidation of a broad variety of chemical compounds. production of laccases by basidiomycetes has been broadly studied as they secrete the enzymes, grow on cheap substrates, and they generally produce more than one isoenzyme (constitutive and/or inducible). laccase production and isoenzyme profile can be modified through medium composition and the use of inducers. the objective of this work was to increase l ...201120694851
antioxidant properties and phenolic profile of the most widely appreciated cultivated mushrooms: a comparative study between in vivo and in vitro samples.the present study reports a comparison of the antioxidant properties and phenolic profile of the most consumed species as fresh cultivated mushrooms and their mycelia produced in vitro: agaricus bisporus (white and brown), pleurotus ostreatus (oyster), pleurotus eryngii (king oyster) and lentinula edodes (shiitake). the antioxidant activity was evaluated through reducing power (folin-ciocalteu and ferricyanide/prussian blue assays), free radical scavenging activity (dpph assay) and lipid peroxid ...201222369965
preparation of culinary-medicinal king oyster mushroom pleurotus eryngii-fermented products with high ergothioneine content and their taste quality.pleurotus eryngii (dc. : fr.) ouel. was used in solid-state fermentation to develop novel mushroom products with a high amount of ergothioneine. the correlation coefficients between ergothioneine content and biomass were 0.8878 and 0.9206 for fermented adlay and buckwheat, respectively. using optimal conditions, pleurotus-fermented adlay and buckwheat (pfa and pfb) with the ergothioneine contents of 795.5 and 786.1 mg/ kg, respectively, were prepared. however, mycelia contained the highest ergot ...201222339711
construction of a genetic linkage map based on amplified fragment length polymorphism markers and development of sequence-tagged site markers for marker-assisted selection of the sporeless trait in the oyster mushroom (pleurotus eryngii).a large number of spores from fruiting bodies can lead to allergic reactions and other problems during the cultivation of edible mushrooms, including pleurotus eryngii (dc.) quél. a cultivar harboring a sporulation-deficient (sporeless) mutation would be useful for preventing these problems, but traditional breeding requires extensive time and labor. in this study, using a sporeless p. eryngii strain, we constructed a genetic linkage map to introduce a molecular breeding program like marker-assi ...201222210222
determination of optimal extraction time and temperature by response surface methodology to obtain high-level antioxidant activity in culinary-medicinal oyster mushroom, pleurotus ostreatus (jacq.:fr.) p. kumm. (higher basidiomycetes).a central composite design of response surface methodology (rsm) was employed to optimize the extraction time (x1: 266.4-393.6 min) and temperature (x2: 42.9-57.1°c) of pleurotus ostreatus aqueous extract with high antioxidant activities, namely dpph radical-scavenging activity, abts radical cation inhibition, and ferric reducing/antioxidant power, as well as total phenolic content (tpc). results showed that the data were adequately fitted into four second-order polynomial models developed by rs ...201223510253
activity of crude cold-water extract of the culinary-medicinal oyster mushroom, pleurotus ostreatus (jacq.:fr.) p.kumm. (higher basidiomycetes), and timolol maleate on induced ocular hypertension.aqueous crude cold-water extract from the fruiting body of the culinary-medicinal oyster mushroom pleurotus ostreatus was assessed for activity against increased intra-ocular pressure (iop) in mice. a 0.1% dexamethasone instillation was used to raise the intra-ocular pressure in the animals above the 21-mmhg threshold limit. the extract has intrinsic anti-hypertensive properties that are dose dependent. a comparison analysis indicated that 150 mg/ml of the crude extract produced 57.69% reduction ...201223510215
oral hypoglycemic activity of culinary-medicinal mushrooms pleurotus ostreatus and p. cystidiosus (higher basidiomycetes) in normal and alloxan-induced diabetic wistar rats.this study investigates the oral hypoglycemic activity of pleurotus ostreatus (p.o.) and p. cystidiosus (p.c.) mushrooms on normal and alloxan-induced diabetic wistar rats. different doses (250, 500, 750, 1000, and 1250 mg/kg/body weight) of suspensions of freeze-dried and powdered (sfdp) p.o. and p.c. were administered to normal rats, and postprandial serum glucose levels were measured. optimal time of activity was investigated using the dose 500 mg/kg. hypoglycemic effect of a single dose of s ...201223510172
self-assembly of hydrophobin protein rodlets studied with atomic force spectroscopy in dynamic mode.we have investigated the self-assembling properties of the class i hydrophobin vmh2 isolated from the fungus pleurotus ostreatus. five different hydrophobin self assembled samples including monolayers, bilayers, and rodlets have been prepared by langmuir technique and studied at the nanoscale. local wettability and visco-elasticity of the different hydrophobins samples were obtained from atomic force spectroscopy experiments in dynamic mode performed at different, controlled relative humidity (r ...201222181848
strategies for dephenolization of raw olive mill wastewater by means of pleurotus ostreatus.the reduction of polyphenols content in olive mill wastewater (omw) is a major issue in olive oil manufacturing. although researchers have pointed out the potential of white-rot fungus in dephenolizing omw, the results available in the literature mainly concern pretreated (sterilized) omw. this paper deals with the reduction of polyphenols content in untreated omw by means of a white-rot fungus, pleurotus ostreatus. dephenolization was performed both in an airlift bioreactor and in aerated flask ...201222179541
effect of gamma and electron beam irradiation on the physico-chemical and nutritional properties of mushrooms: a review.the short shelf-life of mushrooms is an obstacle to the distribution and marketing of the fresh product. thus, prolonging postharvest storage, while preserving their quality, would benefit the mushroom industry as well as consumers. there has been extensive research on finding the most appropriate technology for mushrooms preservation. gamma, electron-beam and uv irradiation have been shown to be potential tools in extending the postharvest shelf-life of fresh mushrooms. studies evaluating the e ...201222868140
novel isolation of water-soluble polysaccharides from the fruiting bodies of pleurotus ostreatus mushrooms.novel water-soluble polysaccharides have been isolated from the fruiting bodies of the edible mushroom pleurotus ostreatus. three polysaccharide fractions were obtained by ethanol precipitation from cold water, hot water and hot aqueous naoh extracts. the fractions were purified by size exclusion chromatography showing a unique carbohydrate occurring in each fraction: pc from the cold fraction, ph from the hot fraction and pb from the hot aqueous naoh fraction. the analysis of the methylated ald ...201222824506
occurrence and accumulation of mercury in two species of wild grown pleurotus mushrooms from southeastern nigeria.the aim of this study was to evaluate the degree of contamination and intake rates, as well as the risk of hg contained in two wild species of pleurotus mushrooms--oyster mushroom (p. ostreatus) and king tuber mushroom (p. tuber-regium)--which are widely consumed in southeastern nigeria; and to also assess their potential to accumulate hg. the mean hg concentrations in caps of p. ostreatus from distant sites of ekeoba, ntigha, and ubakala in abia state, were 31±11, 28±8, and 29±5 ng g(-1) dry we ...201222818845
production, purification and characterization of laccase from pleurotus ostreatus grown on tomato pomace.a strain of pleurotus ostreatus was grown in tomato pomace as sole carbon source for production of laccase. the culture of p. ostreatus revealed a peak of laccase activity (147 u/l of fermentation broth) on the 4th day of culture with a specific activity of 2.8 u/mg protein. differential chromatographic behaviour of laccase was investigated on affinity chromatographic matrices containing either urea, acetamide, ethanolamine or ida as affinity ligands. laccase exhibited retention on such affinity ...201222806800
low-cost and low maintenance preservation of agaricus brasiliensis cultures.agaricus brasiliensis cultures quickly lose viability when stored at cool temperatures, even for a short period of time. we evaluated several low-cost preservation methods using varied substrates, preservation solutions, and storage temperatures. agaricus brasiliensis was intolerant to freezing temperatures, making liquid nitrogen use and deep-freezing methods impossible for its preservation. the best preservation conditions for the a. brasiliensis cs1 strain tested in this study were obtained b ...201222806115
submerged fermentation of the edible mushroom pleurotus ostreatus in a batch stirred tank bioreactor as a promising alternative for the effective production of bioactive metabolites.the aim of this study was to investigate the potential of the submerged fermentation procedure in the production of bioactive metabolites of the common edible mushroom pleurotus ostreatus. the biomass of the mushroom strain was produced by submerged fermentation in a batch stirred tank bioreactor and extracted by solvents of increasing polarity. the dichloromethane and methanol extract were fractioned by different techniques including adsorption chromatography and fast centrifugal partition chro ...201222395402
pcbs stimulate laccase production and activity in pleurotus ostreatus thus promoting their removal.pleurotus ostreatus degrades polychlorinated biphenyls (pcbs) with an increase of laccase activity. laccases are well known for their detoxifying activity. we show, using reverse transcription polymerase chain reaction and a biochemical assay, that reduction in pcbs (di, tri, tetra, and penta) levels are correlated with an increase in laccase activity. p. ostreatus cultures were obtained from 0 to 30 days in the presence or absence of 7,100 mg/l pcbs (from transformer oil) and a surfactant. afte ...201222388978
changes in the activities of enzymes involved in the degradation of butylbenzyl phthalate by pleurotus ostreatus.degradation of butylbenzyl phthalate (bbp) by the white rot fungus pleurotus ostreatus and the activities of some degrading enzymes were examined in two different media containing 100 mg/l of the compound. p. ostreatus pregrown for 7 days in complex ymg medium was able to completely degrade bbp within an additional 24 h but degraded only 35 mg/l of bbp in 5 days of incubation in minimal medium. fungal cell mass in the culture in ymg medium was higher in the presence than in the absence of bbp. t ...201222370356
hypolipidemic and antiatherogenesis effect of culinary-medicinal pink oyster mushroom, pleurotus salmoneostramineus l. vass. (higher basidiomycetes), in hypercholesterolemic rats.the present study was conducted to investigate dietary supplementation of pleurotus salmoneostramineus fruiting bodies on biochemical and histological effects in hyper- and normocholesterolemic rats. six-week-old female sprague-dawley albino rats were divided into three groups of 10 rats each. feeding of diet containing a 5% powder of the fruiting bodies of p. salmoneostramineus in hypercholesterolemic rats reduced plasma total cholesterol, triglyceride, low-density lipoprotein, total lipid, pho ...201222339705
biodecontamination of water from bisphenol a using ligninolytic fungi and the modulation role of humic acids.bisphenol a (bpa) is an endocrine disruptor compound (edc) of xenobiotic origin occurring in natural waters and wastewaters, especially in the most industrialized and urbanized areas. recent investigations report the use of ligninolytic fungi for the removal of aromatic contaminants, including some edcs, from different matrices. humic acids (ha) are widely spread in all natural systems and their presence is ascertained to interfere with microbial growth and activity. the objective of this study ...201222305120
environmental conditions modulate the switch among different states of the hydrophobin vmh2 from pleurotus ostreatus.fungal hydrophobins are amphipathic, highly surface-active, and self-assembling proteins. the class i hydrophobin vmh2 from the basidiomycete fungus pleurotus ostreatus seems to be the most hydrophobic hydrophobin characterized so far. structural and functional properties of the protein as a function of the environmental conditions have been determined. at least three distinct phenomena can occur, being modulated by the environmental conditions: (1) when the ph increases or in the presence of ca ...201222292968
production, purification and characterisation of polysaccharides from pleurotus ostreatus with antitumour activity.mushroom polysaccharides play an important role in functional foods because they exhibit biological modulator properties such as antitumour, antiviral and antibacterial activities. the present study involved the production, purification and characterisation of intracellular and extracellular free and protein-bound polysaccharides from pleurotus ostreatus and the investigation of their growth-inhibitory effect on human carcinoma cell lines.201222234986
effect of β-1.3/1.6-d-glucan derived from oyster mushroom pleurotus ostreatus on biometrical, haematological, biochemical, and immunological indices in rainbow trout (oncorhynchus mykiss).effect of long-term oral administration of three different concentrations (0.5, 1.0, and 2.0%) of micronized β-1.3/1.6-d-glucan derived from oyster mushroom (pleurotus ostreatus, hiratake) on biometrical, haematological, biochemical, and immunological indices of half-year-old rainbow trout (oncorhynchus mykiss) was assessed in the study.201223353851
the microbial population in the air of cultivation facility of oyster mushrooms.the microbial population in the air of mushroom cultivation facility was studied to understand the population structure and size depending on the cultivation methods and regions. the air contents of ten farmers' oyster mushroom cultivation facilities in kyunggi province were sampled. the results indicated that there was no difference in population size depending on the regions of mushroom cultivation. in addition, the population size of bacteria in the growth room was bigger than that of the coo ...201223274995
biodegradation of vegetable and agrowastes by pleurotus sapidus: a novel strategy to produce mushroom with enhanced yield and nutrition.edible oyster mushroom pleurotus sapidus was cultivated, on pea pod shell, cauliflower leaves, radish leaves, brassica straw in various combinations of paddy straw. the mushroom failed to grow on these vegetable wastes separately. however, it grew very well on these vegetable wastes when mixed with various combinations of paddy straw as substrate. total yield and biological efficiency of the mushroom cultivated on substrate containing 20% and 30% vegetable wastes mixed with 70% and 80% (w/w) of ...201223273184
mechanistic study of 17α-ethinylestradiol biodegradation by pleurotus ostreatus: tracking of extracelullar and intracelullar degradation mechanisms.the white rot fungus pleurotus ostreatus is able to completely remove the synthetic hormone 17α-ethinylestradiol (ee2, 200 μg in 20 ml) from a liquid complex or mineral medium in 3 or 14 days, respectively. its efficiency has also been documented in the removal of estrogenic activity that correlated with the ee2 degradation. a set of in vitro experiments using various cellular and enzyme fractions has been performed and the results showed that ee2 was degraded by isolated laccase (about 90% with ...201223150991
[overexpression and characterization of a laccase gene from pleurotus ostreatus in trichoderma reesei].expression, purification and characterization of a laccase gene from pleurotus ostreatus in trichoderma reesei.201223115969
the impact of pleurotus ostreatus on organic matter transformation processes.this study showed the effect of pleurotus ostreatus on the external organic matter (om) transformation processes. the kinetics of these changes were determined. the experiment was conducted as 42-day pot experiment done in four combinations (with four replications). the four combinations of different substrates were: (1) organic, (2) composted hay, (3) organic soil + composted hay, (4) organic soil + composted hay + lead. the study results showed that p. ostreatus had the ability to transform ex ...201223109584
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