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nitrogen compounds in the apoplastic sap of sugarcane stem: some implications in the association with endophytes.several nitrogen compounds were identified and quantified in the apoplastic and symplastic sap of sugarcane stems. the sap of stems was composed mainly of soluble sugars, which constituted 95% of the total organic compounds detected. sap also contained nitrogen compounds, with amino acids (50-70% of n) and proteins (20-30% of n), being the main nitrogenous substances, as well as inorganic forms as ammonium, nitrite and nitrate, in low concentrations (<20% of n). serine, proline, alanine and aspa ...200616360806
xylitol production from corn fiber and sugarcane bagasse hydrolysates by candida tropicalis.a natural isolate, candida tropicalis was tested for xylitol production from corn fiber and sugarcane bagasse hydrolysates. fermentation of corn fiber and sugarcane bagasse hydrolysate showed xylose uptake and xylitol production, though these were very low, even after hydrolysate neutralization and treatments with activated charcoal and ion exchange resins. initial xylitol production was found to be 0.43 g/g and 0.45 g/g of xylose utilised with corn fiber and sugarcane bagasse hydrolysate respec ...200616242318
gluconacetobacter diazotrophicus elicits a sugarcane defense response against a pathogenic bacteria xanthomonas albilineans.a new role for the plant growth-promoting nitrogen-fixing endophytic bacteria gluconacetobacter diazotrophicus has been identified and characterized while it is involved in the sugarcane-xanthomonas albilineans pathogenic interactions. living g.diazotrophicus possess and/or produce elicitor molecules which activate the sugarcane defense response resulting in the plant resistance to x. albilineans, in this particular case controlling the pathogen transmission to emerging agamic shoots. a total of ...200619516988
variation in albicidin biosynthesis genes and in pathogenicity of xanthomonas albilineans, the sugarcane leaf scald pathogen.abstract total genomic dna from 137 strains of xanthomonas albilineans from worldwide locations was hybridized with two dna probes that together harbor the entire 49-kb albicidin biosynthesis gene cluster and two additional 3-kb genomic regions required for albicidin production. fourteen haplotypes and two major genetic groups (albicidin [alb]-restriction fragment length polymorphism [rflp] a and alb-rflp b) were identified, and strains that were isolated after recent outbreaks of leaf scald dis ...200618944203
high variation in pathogenicity of genetically closely related strains of xanthomonas albilineans, the sugarcane leaf scald pathogen, in guadeloupe.abstract pathogenicity of 75 strains of xanthomonas albilineans from guadeloupe was assessed by inoculation of sugarcane cv. b69566, which is susceptible to leaf scald, and 19 of the strains were selected as representative of the variation in pathogenicity observed based on stalk colonization. in vitro production of albicidin varied among these 19 strains, but the restriction fragment length polymorphism pattern of their albicidin biosynthesis genes was identical. similarly, no genomic variation ...200618943496
[screening of microorganism producing polyunsaturated fatty acids with sugarcane molasses].a strain name lb1 producing polyunsaturated fatty acids with sugarcane molasses was screened form soil samples by the methods of sudan black b stain and the determination of pufas' iodine value. based on the grass morphological and physiological characteristics and the sequence similarity of ribosomal dna-its, the strain was identified as mucor recurvus sp.. the results of l64 (4(3)) orthogonal experiments indicated when mucor recurvus was cultivated at 28 degrees c for 5 days with 160 r/min, th ...200617037053
determination of flavonoids in cultivated sugarcane leaves, bagasse, juice and in transgenic sugarcane by liquid chromatography-uv detection.a high-performance liquid chromatography (hplc) method with photo-diode array (dad) detection was developed to separate and quantify flavonoids in sugarcane leaves and bagasse (= the crushed sugarcane refuse from juice extraction), and in sugarcane juice. sugarcane flavonoids consist of a complex mixture of aglycones and glycosides (including flavonolignan glycosides), and the hplc-uv method herein proposed is suitable for their quantification as total flavonoids. this method was applied to anal ...200616310199
development of an integrated genetic map of a sugarcane (saccharum spp.) commercial cross, based on a maximum-likelihood approach for estimation of linkage and linkage phases.sugarcane (saccharum spp.) is a clonally propagated outcrossing polyploid crop of great importance in tropical agriculture. up to now, all sugarcane genetic maps had been developed using either full-sib progenies derived from interspecific crosses or from selfing, both approaches not directly adopted in conventional breeding. we have developed a single integrated genetic map using a population derived from a cross between two pre-commercial cultivars ('sp80-180' x 'sp80-4966') using a novel appr ...200616307229
effects of genetic polymorphisms cyp1a1, gstm1, gstt1 and gstp1 on urinary 1-hydroxypyrene levels in sugarcane workers.sugarcane workers in brazil are exposed to various genotoxic compounds, including polycyclic aromatic hydrocarbons (pahs), derived from an incomplete combustion process of burnt sugarcane fields. the effects of the occupational exposure to sugarcane fields burning were measured in urine samples of sugarcane workers from the northwest of the state of são paulo when exposed (harvesting) and when non-exposed (non-harvesting). the urinary levels of 1-hydroxypyrene (1-ohp) and the influence of the ge ...200616914185
ultrastructural study of the ovary of the sugarcane spittlebug mahanarva fimbriolata (hemiptera).the present study describes the ultrastructure of meroistic telotrophic ovaries of the sugarcane spittlebug mahanarva fimbriolata. in this type of ovary, nurse cells, oogonia, and prefollicular tissue are located at the terminal (distal) regions or tropharium of ovarioles. oocytes in different developmental stages, classified from i to v, are observed in the vitellarium. stage i oocytes do not exhibit intercellular spaces in the follicular epithelium, suggesting that synthesis and production of ...200616644228
comparison of stir bar sorptive extraction and membrane-assisted solvent extraction as enrichment techniques for the determination of pesticide and benzo[a]pyrene residues in brazilian sugarcane juice.the present work compares two modern and environmentally friendly enrichment techniques, stir bar sorptive extraction (sbse) and membrane-assisted solvent extraction (mase) for the determination of 18 organic contaminant residues in brazilian sugarcane juice. stir bar sorptive extraction and thermal desorption coupled to capillary gas chromatography-mass spectrometry using the selected ion monitoring mode [sbse-td-gc-ms(sim)] and membrane-assisted solvent extraction combined with large volume in ...200616597442
ultrastructural study of the salivary glands of the sugarcane spittlebug mahanarva fimbriolata (stal, 1854) (euhemiptera: cercopidae).spittlebugs are insects that suck sap from plants and regurgitate saliva containing toxic enzymes into the leaves. as a consequence, the conductive channels are blocked resulting in dry leaves, thus giving a burned aspect to the plantation. this work performed ultrastructural analyses of the salivary glands of the sugarcane spittlebug mahanarva fimbriolata, since these organs produce the enzymes that are injected into the plants, thus being responsible for the economic losses in the production o ...200616171997
morphological aspects of cluster formation in the germarium of the sugarcane borer diatraea saccharalis fabricius (lepidoptera: pyralidae).diatraea saccharalis f. is one of the greatest pests of the sugar cane culture. this report aimed to characterize the germarium region of the sugarcane borer by light and transmission electron microscopy, emphasizing the morphological steps of the ovarian cluster formation. in the germarium of this insect, four zones could be morphologically identified during the cluster formation. in the most apical end of each ovariole--zone i--the germ line stem cells undergo complete mitotic division, origin ...200617144137
resistance to stem borers (lepidoptera: crambidae) among texas rice cultivars.a 4-yr field study was conducted to assess the resistance of rice, oryza sativa l., cultivars to injury from the sugarcane borer, diatraea saccharalis (f.), and the mexican rice borer, eoreuma loftini (dyar) (both lepidoptera: crambidae). several cultivars showed reduced levels of injury ('clxl8', 'xl8', 'wells', 'cheniere', and 'xp710' in 2003; clxl8, xp723, cheniere, and 'cl161' in 2004) and lower stem borer yield loss (clxl8 in 2004) than the more susceptible 'priscilla'. the resistant clxl8 ...200617066824
sugarcane borer (lepidoptera: crambidae) management threshold assessment on four sugarcane cultivars.this research assesses the potential for using different economic injury thresholds in management of a key insect pest on susceptible and resistant commercially produced cultivars of sugarcane (saccharum spp. hybrids). in a 2-yr sugarcane borer, diatraea saccharalis (f.) (lepidoptera: crambidae), study involving four commercially produced sugarcane cultivars and four insecticide treatment thresholds, 'lcp 85-384' and 'hocp 91-555' were the most susceptible based on percentage of bored internodes ...200616813338
the plant energy-dissipating mitochondrial systems: depicting the genomic structure and the expression profiles of the gene families of uncoupling protein and alternative oxidase in monocots and dicots.the simultaneous existence of alternative oxidases and uncoupling proteins in plants has raised the question as to why plants need two energy-dissipating systems with apparently similar physiological functions. a probably complete plant uncoupling protein gene family is described and the expression profiles of this family compared with the multigene family of alternative oxidases in arabidopsis thaliana and sugarcane (saccharum sp.) employed as dicot and monocot models, respectively. in total, s ...200616473895
bioactivity of neem, azadirachta indica, against spittlebug mahanarva fimbriolata (hemiptera: cercopidae) on sugarcane.the effect of neem, azadirachta indica a. juss, on some biological parameters of mahanarva fimbriolata (stil) (hemiptera: cercopidae) was studied in the laboratory by using neemazal-t/s, nimkol-ls, and an aqueous neem seed extract. initially, the lc,, was estimated for nymphs. later, nymphs fed on sugarcane, saccharum officinarum l., roots were sprayed with the respective lc,, for each product. the adults were maintained in cages on sugarcane plants sprayed at the base with the maximum rate reco ...200617195667
unraveling evolutionary relationships among the divergent lineages of colletotrichum causing anthracnose disease in turfgrass and corn.abstract colletotrichum species cause anthracnose diseases on a number of grass hosts and are common inhabitants of many others. they are divided into four species: c. sublineolum is pathogenic to sorghum spp.; c. caudatum is found on c4 grasses such as indiangrass and big bluestem; c. falcatum causes red rot of sugarcane; and c. graminicola sensu lato is a broadly defined species including isolates that attack maize, wheat, oats, and many forage, turf, and amenity grasses of the subfamily pooid ...200618944204
assessment of herbicide leaching risk in two tropical soils of reunion island (france).application of organic chemicals to a newly irrigated sugarcane (saccharum officinarum l.) area located in the semiarid western part of reunion island has prompted local regulatory agencies to determine their potential to contaminate ground water resources. for that purpose, simple indices known as the ground water ubiquity score (gustafson index, gus), the retardation factor (rf), the attenuation factor (af), and the log-transformed attenuation factor (aft) were employed to assess the potential ...200615758106
pcdd and pcdf emissions from simulated sugarcane field burning.the emissions from simulated sugarcane (saccharum officinarum) field burns were sampled and analyzed for polychlorinated dibenzodioxins and dibenzofurans (pcdds and pcdfs). sugarcane leaves from hawaii and florida were burned in a manner simulating the natural physical dimensions and biomass density found during the practice of preharvest field burning. eight composite burn tests consisting of 3-33 kg of biomass were conducted, some with replicate samplers. emission factor calculations using sam ...200617120546
genetic segregation of microsatellite markers in saccharum officinarum and s. spontaneum.genetic mapping techniques can be used to study the interaction between two different genomes after hybridization. this study investigated a saccharum officinarum (green german or gg, 2n approximately 11x approximately 110) x s. spontaneum (ind 81-146 or ind, 2n approximately 7x approximately 56) interspecific cross. segregation of 193 microsatellite (ssr) loci was evaluated in the f(1) progeny of 169 full-sibs of the cross. following the two-way pseudo-testcross strategy and 'cross pollination' ...200616912699
plant growth inhibitors isolated from sugarcane (saccharum officinarum) straw.several compounds related with plant defense and pharmacological activities have been isolated from sugarcane. straw phytotoxins and their possible mechanisms of growth inhibition are largely unknown. a bioassay-guided fractionation of the phytotoxic constituents leachated from a sugarcane straw led to the isolation of trans-ferulic (trans-fa), cis-ferulic (cis-fa), vanillic (va) and syringic (sa) acids. the straw leachates and their identified constituents significantly inhibited root growth of ...200616777531
quantitative trait loci identified for sugar related traits in a sugarcane (saccharum spp.) cultivar x saccharum officinarum population.the identification of markers linked to quantitative trait loci (qtls) for increased sugar accumulation could improve the effectiveness of current breeding strategies in sugarcane. progeny from a cross between a high sucrose producing cultivar, (denotes australian plant breeding rights), and a saccharum officinarum clone, ij76-514 were grown in two field experiments in different years, and evaluated in the early and mid-season phases of crop maturity, to identify robust qtls in affecting sucrose ...200616508765
physiological implications of metabolite biosynthesis for net assimilation and heat-stress tolerance of sugarcane (saccharum officinarum) sprouts.global increase in ambient temperature is a critical factor for plant growth. in order to study the changes in growth over short intervals, various primary and secondary metabolites, and their relationships with thermotolerance, 1-month-old sugarcane (saccharum officinarum) sprouts were grown under control conditions (28 degrees c) or under heat-stress conditions (40 degrees c), and measurements were made at six 12-h intervals. heat stress greatly reduced dry matter and leaf area of sprouts init ...200717024517
genetic analysis for brix weight per stool and its component traits in sugarcane (saccharum officinarum).brix weight per stool (bw) of sugarcane is a complex trait, which is the final product of a combination of many components. diallel cross experiments were conducted during a period of two years for bw and its five component traits, including stalk diameter (sd), stalk length (sl), stalk number (sn), stalk weight (sw), and brix scale (bs) of sugarcane. phenotypic data of all the six traits were analyzed by mixed linear model and their phenotype variances were portioned into additive (a), dominanc ...200718257118
construction of a genetic linkage map for saccharum officinarum incorporating both simplex and duplex markers to increase genome coverage.saccharum officinarum l. is an octoploid with 80 chromosomes and a basic chromosome number of x = 10. it has high stem sucrose and contributes 80% of the chromosomes to the interspecific sugarcane cultivars that are grown commercially for sucrose. a genetic linkage map was developed for s. officinarum (clone ij76-514) using a segregating population generated from a cross between q165 (a commercial sugarcane cultivar) and ij76-514. in total, 40 aflp and 72 ssr primer pairs were screened across th ...200717893734
domestication to crop improvement: genetic resources for sorghum and saccharum (andropogoneae).both sorghum (sorghum bicolor) and sugarcane (saccharum officinarum) are members of the andropogoneae tribe in the poaceae and are each other's closest relatives amongst cultivated plants. both are relatively recent domesticates and comparatively little of the genetic potential of these taxa and their wild relatives has been captured by breeding programmes to date. this review assesses the genetic gains made by plant breeders since domestication and the progress in the characterization of geneti ...200717766842
kinetic model of sucrose accumulation in maturing sugarcane culm tissue.biochemically, it is not completely understood why or how commercial varieties of sugarcane (saccharum officinarum) are able to accumulate sucrose in high concentrations. such concentrations are obtained despite the presence of sucrose synthesis/breakdown cycles (futile cycling) in the culm of the storage parenchyma. given the complexity of the process, kinetic modelling may help to elucidate the factors governing sucrose accumulation or direct the design of experimental optimisation strategies. ...200717555779
low cost sorbents for the removal of methyl parathion pesticide from aqueous solutions.sorptive potential of selected agricultural waste materials i.e. rice (oryza sativa) bran (rb), bagasse fly ash (bfa) of sugarcane (saccharum officinarum), moringa oleifera pods (mop) and rice husk (rh) for the removal of methyl parathion pesticide (mp) from surface and ground waters has been investigated. optimization of operating parameters of sorption process, i.e. sorbent dose, agitation time, ph, initial concentration of sorbate, and temperature have been studied. the sorption data fitted t ...200717109916
complete chloroplast genome sequences of hordeum vulgare, sorghum bicolor and agrostis stolonifera, and comparative analyses with other grass genomes.comparisons of complete chloroplast genome sequences of hordeum vulgare, sorghum bicolor and agrostis stolonifera to six published grass chloroplast genomes reveal that gene content and order are similar but two microstructural changes have occurred. first, the expansion of the ir at the ssc/ira boundary that duplicates a portion of the 5' end of ndhh is restricted to the three genera of the subfamily pooideae (agrostis, hordeum and triticum). second, a 6 bp deletion in ndhk is shared by agrosti ...200717534593
expression and variability of molecular chaperones in the sugarcane expressome.molecular chaperones perform folding assistance in newly synthesized polypeptides preventing aggregation processes, recovering proteins from aggregates, among other important cellular functions. thus their study presents great biotechnological importance. the present work discusses the mining for chaperone-related sequences within the sugarcane est genome project database, which resulted in approximately 300 different sequences. since molecular chaperones are highly conserved in most organisms s ...200716687190
efficient isolation of anthraquinone-derivatives from trichoderma harzianum ets 323.anthraquinone-derivatives, chrysophanol and pachybasin, were purified by a silica column chromatography with two different solvent systems from trichoderma harzianum ets 323. the fungus was incubated in sugarcane bagasse solid medium at room temperature without rotation. structure of chrysophanol was solved by x-ray diffraction and pachybasin by nmr spectra. about 233+/-13 mg of pure chrysophanol and 773+/-40 mg of pure pachybasin were recovered per kg of solid cultural medium, with yields 1.7+/ ...200717067682
histochemical and ultrastructural evidence of lipid secretion by the silk gland of the sugarcane borer diatraea saccharalis (fabricius) (lepidoptera: crambidae).the silk gland in lepidoptera larvae is responsible for the silk production used for shelter or cocoon construction. the secretion of fibroin and sericin by the different silk gland regions are well established. there are few attempts to detect lipid components in the insect silk secretion, although the presence of such element may contribute to the resistance of the shelter to wet environment. this study characterizes the glandular region and detects the presence of lipid components in the secr ...200718060296
water stress augments silicon-mediated resistance of susceptible sugarcane cultivars to the stalk borer eldana saccharina (lepidoptera: pyralidae).silicon (si) can improve resistance of plants to insect attack and may also enhance tolerance of water stress. this study tested if si-mediated host plant resistance to insect attack was augmented by water stress. four sugarcane cultivars, two resistant (n21, n33) and two susceptible (n26, n11) to eldana saccharina walker were grown in a pot trial in si-deficient river sand, with (si+) and without (si-) calcium silicate. to induce water stress, irrigation to half the trial was reduced after 8.5 ...200717411480
antibacterial activity and phytochemical evidence for the plant origin of turkish propolis from different regions.honeybees collect propolis from practically any abundant plant source in the neighborhood of the hive, be it populus, eucalyptus, pine, sugarcane, cashew nut or orange trees. we have described that the origin plants of turkish propolis are populus sp., eucalyptus sp. and castanea sativa. in our previous study, propolis samples from middle anatolia displayed the typical pattern of "poplar" propolis: they contained pinobanksin, caffeic and ferulic acids and their esters. the propolis samples exami ...200727517836
resource recovery potential from secondary components of segregated municipal solid wastes.fermentable components of municipal solid wastes (msw) such as fruit and vegetable wastes (fvw), leaf litter, paddy straw, cane bagasse, cane trash and paper are generated in large quantities at various pockets of the city. these form potential feedstocks for decentralized biogas plants to be operated in the vicinity. we characterized the fermentation potential of six of the above msw fractions for their suitability to be converted to biogas and anaerobic compost using the solid-state stratified ...200717503209
female reproductive system of the sugarcane spittlebug mahanarva fimbriolata (auchenorrhyncha): vitellogenesis dynamics and protein quantification.the present study aimed describing the ovaries of the sugarcane spittlebug mahanarva fimbriolata which are meroistic telotrophic with nurse cells and oocytes located in the tropharium. sem revealed paired ovaries located dorsolaterally around the intestine, and oocytes exhibiting shapes ranging from round (less developed) to elliptic (more developed), suggesting a simultaneous, although, asynchronous development. based on histological data we classified the oocytes in stages from i to v. stage i ...200716973368
carboxymethylcellulose obtained by ethanol/water organosolv process under acid conditions.sugar cane bagasse pulps were obtained by ethanol/water organosolv process under acid and alkaline conditions. the best condition of acid pulping for the sugarcane bagasse was 0.02 mol/l sulfuric acid at 160 degrees c, for 1 h, whereas the best condition for alkaline pulping was 5% sodium hydroxide (base pulp) at 160 degrees c, for 3 h. for the residual lignin removal, the acid and alkaline pulps were submitted to a chemical bleaching using sodium chlorite. pulps under acid and alkaline conditio ...200718478417
leaching and half-life of the herbicide tebuthiuron on a recharge area of guarany aquifer in sugarcane fields in brazil.this study was undertaken to evaluate the degradation and mobility of the herbicide tebuthiuron (n-[5-(1,1-dimethylethyl)-1,3,4-thiadiazol-2-yl]-n,n'-dimethylurea) in soil under field conditions, and its potential for leaching and groundwater contamination. a watershed, espraiado, located over a recharge area in brazil, was chosen for soil and water studies. at espraiado, water samples were collected from seven wells at intervals of three months from march 2004 to june 2006 and analyzed for tebu ...200717701698
identification of genes responsive to the application of ethanol on sugarcane leaves.the control of gene expression in precise time and space is a desirable attribute of chemically inducible systems. ethanol is a chemical inducer with favourable features, such as being inexpensive and easy to apply. the aim of this study was to identify ethanol-responsive genes in sugarcane. the cdna macroarray technique was adopted to identify transcript changes in sugarcane leaves (saccharum spp. cv sp80-3280) exposed to ethanol. the expression profiles of sugarcane genes were analysed using n ...200717701412
pipeline for macro- and microarray analyses.the pipeline for macro- and microarray analyses (pmma) is a set of scripts with a web interface developed to analyze dna array data generated by array image quantification software. pmma is designed for use with single- or double-color array data and to work as a pipeline in five classes (data format, normalization, data analysis, clustering, and array maps). it can also be used as a plugin in the bioarray software environment, an open-source database for array analysis, or used in a local versi ...200717464422
orthologous comparison in a gene-rich region among grasses reveals stability in the sugarcane polyploid genome.modern sugarcane (saccharum spp.) is an important grass that contributes 60% of the raw sugar produced worldwide and has a high biofuel production potential. it was created about a century ago through hybridization of two highly polyploid species, namely s. officinarum and s. spontaneum. we investigated genome dynamics in this highly polyploid context by analyzing two homoeologous sequences (97 and 126 kb) in a region that has already been studied in several cereals. our findings indicate that t ...200717425713
biochemical and biophysical characterization of small heat shock proteins from sugarcane. involvement of a specific region located at the n-terminus with substrate specificity.when cells are submitted to an increase in temperature, heat shock proteins (hsp) are synthesized to help heat stress resistance. small hsps, which are diverse and abundant in plants, have the major function of preventing irreversible protein aggregation. the diversity of small hsps in plants is intriguing and characterization of their chaperone activity is important to understand plant tolerance to heat stress. a previous study showed that small hsps, mainly represented by class i (cytosolic), ...200717336576
mudra-like sequences from rice and sugarcane cluster as two bona fide transposon clades and two domesticated transposases.the mutator system of maize has been described as the most active and mutagenic plant transposon. the autonomous element mudr contains two genes: mudra encoding the transposase and mudrb whose product function remains undetermined. mudra-like coding domain showed to be the most abundantly expressed transposon-related sequence in sugarcane transcriptome. a previous report identified the existence of at least four clades of mudra-like sequences in sugarcane, rice and arabidopsis, which already exi ...200717289300
serial analysis of gene expression in sugarcane (saccharum spp.) leaves revealed alternative c4 metabolism and putative antisense transcripts.sugarcane (saccharum spp.) is a highly efficient biomass and sugar producing crop. leaf reactions have been considered as potential rate-limiting step for sucrose accumulation in sugarcane stalks. to characterize the sugarcane leaf transcriptome, field-grown mature leaves from cultivar "sp80-3280" were analyzed using serial analysis of gene expression (sage). from 480 sequenced clones, 9,482 valid tags were extracted, with 5,227 unique sequences, from which 3,659 (70%) matched at least a sugarca ...200717211512
response of sugarcane to increasing concentrations of copper and cadmium and expression of metallothionein genes.sugarcane (saccharum spp.) offers the potential to be a phytoremediator species due to its outstanding biomass production, but its prospective metal accumulation and tolerance have not been fully characterized. sugarcane plantlets were able to tolerate up to 100microm of copper in nutrient solution for 33 days, with no significant reduction in fresh weight, while accumulating 45mgcukg(-1) shoot dry weight. higher levels of copper in solution (250 and 500microm) were lethal. sugarcane displayed t ...200717175063
relationship between sugarcane rust severity and soil properties in louisiana.abstract the extent of spatial and temporal variability of sugarcane rust (puccinia melanocephala) infestation was related to variation in soil properties in five commercial fields of sugarcane (interspecific hybrids of saccharum spp., cv. lcp 85-384) in southern louisiana. sugarcane fields were grid-soil sampled at several intensities and rust ratings were collected at each point over 6 to 7 weeks. soil properties exhibited significant variability (coefficients of variation = 9 to 70.1%) and we ...200718943606
enzymatic hydrolysis optimization to ethanol production by simultaneous saccharification and fermentation.there is tremendous interest in using agro-industrial wastes, such as cellulignin, as starting materials for the production of fuels and chemicals. cellulignin are the solids, which result from the acid hydrolysis of the sugarcane bagasse. the objective of this work was to optimize the enzymatic hydrolysis of the cellulose fraction of cellulignin, and to study its fermentation to ethanol using saccharomyces cerevisiae. cellulose conversion was optimized using response surface methods with ph, en ...200718478383
dilute sulfuric acid pretreatment of agricultural and agro-industrial residues for ethanol production.the potential of dilute-acid prehydrolysis as a pretreatment method for sugarcane bagasse, rice hulls, peanut shells, and cassava stalks was investigated. the prehydrolysis was performed at 122 degrees c during 20, 40, or 60 min using 2% h(2)so(4) at a solid-to-liquid ratio of 1:10. sugar formation increased with increasing reaction time. xylose, glucose, arabinose, and galactose were detected in all of the prehydrolysates, whereas mannose was found only in the prehydrolysates of peanut shells a ...200718478400
estimation of temperature dependent parameters of a batch alcoholic fermentation process.in this work, a procedure was established to develop a mathematical model considering the effect of temperature on reaction kinetics. experiments were performed in batch mode in temperatures from 30 to 38 degrees c. the microorganism used was saccharomyces cerevisiae and the culture media, sugarcane molasses. the objective is to assess the difficulty in updating the kinetic parameters when there are changes in fermentation conditions. we conclude that, although the re-estimation is a time-consum ...200718478432
long-term carcinogenicity of d-003, a mixture of high molecular weight acids from sugarcane wax, in sprague dawley rats: a 24 months study.d-003 is a mixture of high molecular weight sugarcane wax aliphatic primary acids with cholesterol-lowering, anti-platelet and antioxidant effects. this study investigated the long-term oral toxicity and carcinogenicity of d-003 in sprague dawley rats of both sexes, randomly distributed into four groups: a control group, treated only with the vehicle, and three treated with d-003 (50, 500 and 1500 mg/kg). all treatments were given orally for 24 months. mortality (survival analysis), clinical sym ...200717937974
phenolic compounds from sugarcane molasses possessing antibacterial activity against cariogenic bacteria.during the course of our research into the use of cane by-products from sugar manufacturing, we have studied the isolation and structural determination of bioactive compounds present in sugarcane molasses. in this study, dehydrodiconiferylalcohol-9'-o-beta-d-glucopyranoside (1) and isoorientin-7, 3'-o-dimethyl ether (2) were isolated as antibacterial active compounds against cariogenic bacteria. their structures were elucidated by (1)h-nmr, (13)c-nmr and esi-ms. the activities of these isolated ...200717938552
evaluation of transgenic bacillus thuringiensis corn hybrids against cry1ab-susceptible and -resistant sugarcane borer (lepidoptera: crambidae).a louisiana strain of the sugarcane borer, diatraea saccharalis (f.) (lepidoptera: crambidae), was selected for resistance to the crylab protein of bacillus thuringiensis (bt) by using an f2 screening procedure. survival of bt-resistant, -susceptible, and -heterozygous genotypes of sugarcane borer was evaluated on vegetative and reproductive stages of five non-bt and seven bt field corn, zea mays l., hybrids in a greenhouse study. larval survival was recorded 21 d after infestation of neonates o ...200718232406
use of complex media for the production of scleroglucan by sclerotium rolfsii mtcc 2156.submerged fermentation was carried out for the production of scleroglucan by sclerotium rolfsii mtcc 2156 using complex media, such as coconut water, sugarcane molasses and sugarcane juice at 28+/-2 degrees c and 180 rpm for 72 h. sugarcane juice gave maximum scleroglucan production of 23.87 g/l as compared to 12.58 and 18.45 g/l with coconut water and sugarcane molasses, respectively. utilization of these substrates would be ecologically sound and economically advantageous.200716822667
characterization of poly(3-hydroxybutyrate) produced by cupriavidus necator in solid-state fermentation.solid-state fermentation (ssf) has recently been proposed as an alternative to submerged fermentation for the production of poly(hydroxyalkanoates). in the present work, x-ray diffraction, differential scanning calorimetry, nuclear magnetic resonance and infrared spectroscopy were employed to investigate the chemical structure, as well as the thermal properties and the crystalline morphology of poly(3-hydroxybutyrate) samples produced by ssf, using as raw material either soy cake or soy cake sup ...200716580194
adaptation of a recombinant xylose-utilizing saccharomyces cerevisiae strain to a sugarcane bagasse hydrolysate with high content of fermentation inhibitors.adaptation of a xylose-utilizing genetically engineered strain of saccharomyces cerevisiae to sugarcane bagasse hydrolysates by cultivation during 353h using medium with increasing concentrations of inhibitors, including phenolic compounds, furaldehydes and aliphatic acids, led to improved performance with respect to ethanol production. the remaining xylose concentration in the medium at the end of the cultivation was 5.2g l(-1), while it was 11gl(-1) in the feed, indicating that approximately h ...200716934451
use of post-harvest sugarcane residue for ethanol production.agricultural residues are produced in large quantities throughout the world. approximately, 1kg of residue is produced for each kilogram of grains harvested. this ratio of grain/residue translates into an excess of 40 billion ton of crop residue produced each year in the usa. these residues are renewable resources that could be used to produce ethanol and many other value added products. in this study, we demonstrate that the post-harvest sugar cane residue could be used to produce fuel grade et ...200716935500
biotechnological production of xylitol in a three-phase fluidized bed bioreactor with immobilized yeast cells in ca-alginate beads.cells of candida guilliermondii entrapped in ca-alginate beads were used for xylitol production, from concentrated hemicellulose hydrolyzate of sugarcane bagasse, in a fluidized bed bioreactor (fbr). the maximum xylitol concentration 28.9 g xylitol/l was obtained at a high aeration rate of 600 ml/min after 70 h of fermentation, indicating that the use of high aeration rate in this system is favored for better oxygen transfer into the immobilized cells. the specific xylitol productivity and the x ...200717427994
influence of inhibitory compounds and minor sugars on xylitol production by debaryomyces hansenii.to obtain in-depth information on the overall metabolic behavior of the new good xylitol producer debaryomyces hansenii ufv-170, batch bioconversions were carried out using semisynthetic media with compositions simulating those of typical acidic hemicellulose hydrolysates of sugarcane bagasse. for this purpose, we used media containing glucose (4.3-6.5 g/l), xylose (60.1-92.1 g/l), or arabinose (5.9-9.2 g/l), or binary or ternary mixtures of them in either the presence or absence of typical inhi ...200717496338
potential of plant extracts in combination with bacterial antagonist treatment as biocontrol agent of red rot of sugarcane.plant extracts and antifungal microorganisms were tested singly and in combination for biocontrol of sugarcane red rot disease (colletotrichum falcatum) using two sugarcane (saccharum officinarum l.) cultivars, coc671 and coc92061, in pot and field experiments. leaf extracts of abrus precatorius and bassia latifolia and the rhizome extract of curcuma longa reduced colletotrichum falcatum mycelial growth by 80%, 58%, and 57%, respectively. although sugarcane- planting materials (setts) treated in ...200717496967
bioformulation of burkholderia sp. mssp with a multispecies consortium for growth promotion of cajanus cajan.the present work was undertaken to formulate an effective bioformulation using burkholderia sp. strain mssp, a known plant-growth-promoting rhizobacterium. mssp was tagged with the reporter gene of green fluorescent protein (gfp) to monitor its population in cost-effective solid carriers, including sugarcane-bagasse, sawdust, cocoa peat, rice husk, wheat bran, charcoal, and rock phosphate, and paneer-whey as liquid carrier. physical and chemical properties of different low-cost carrier materials ...200717496969
sugarcane biopolymer patch in femoral artery angioplasty on dogs.the objective of this study was to evaluate the use of the sugarcane biopolymer membrane in femoral artery patch angioplasty on dogs.200717505660
biotechnological production of gluconic acid: future implications.gluconic acid (ga) is a multifunctional carbonic acid regarded as a bulk chemical in the food, feed, beverage, textile, pharmaceutical, and construction industries. the favored production process is submerged fermentation by aspergillus niger utilizing glucose as a major carbohydrate source, which accompanied product yield of 98%. however, use of ga and its derivatives is currently restricted because of high prices: about us$ 1.20-8.50/kg. advancements in biotechnology such as screening of micro ...200717525864
production of d-lactic acid from sugarcane molasses, sugarcane juice and sugar beet juice by lactobacillus delbrueckii.lactobacillus delbrueckii was grown on sugarcane molasses, sugarcane juice and sugar beet juice in batch fermentation at ph 6 and at 40 degrees c. after 72 h, the lactic acid from 13% (w/v) sugarcane molasses (119 g total sugar l(-1)) and sugarcane juice (133 g total sugar l(-1)) was 107 g l(-1) and 120 g l(-1), respectively. with 10% (w/v) sugar beet juice (105 g total sugar l(-1)), 84 g lactic acid l(-1) was produced. the optical purities of d: -lactic acid from the feedstocks ranged from 97.2 ...200717541505
molecular cloning and biochemical characterization of a family-9 endoglucanase with an unusual structure from the gliding bacteria cytophaga hutchinsonii.cytophaga hutchinsonii was originally isolated from sugarcane piles. this microorganism therefore probably produces an array of enzymes allowing it to digest cellulosic substrates. c. hutchinsonii thus represents a rich source of potentially effective cellulase enzymes that can be harnessed for conversion of biomass to simple sugars. these sugars can then be used as feedstock for ethanol production or other chemical syntheses. in this study, we report the pcr cloning of an endoglucanase gene (ce ...200717625271
substrate specificity-conferring regions of the nonribosomal peptide synthetase adenylation domains involved in albicidin pathotoxin biosynthesis are highly conserved within the species xanthomonas albilineans.albicidin is a pathotoxin produced by xanthomonas albilineans, a xylem-invading pathogen that causes leaf scald disease of sugarcane. albicidin is synthesized by a nonribosomal pathway via modular polyketide synthase and nonribosomal peptide synthetase (nrps) megasynthases, and nrps adenylation (a) domains are responsible for the recognition and activation of specific amino acid substrates. dna fragments (0.5 kb) encoding the regions responsible for the substrate specificities of six albicidin n ...200717630307
synthesis of [3-(3-chloro-4-fluorophenyl)-2-oxo-3,4-dihydro-2h-2lambda5- benzo[e][1,3,2]oxazaphosphinin-2-yl]-(aryl/alkyl) methanols and their bioactivity on sugarcane smut.synthesis of some new substituted [3-(3-chloro-4-fluorophenyl)-2-oxo-3,4-dihydro-2h-2lambda(5)-benzo[e][1,3,2]oxazaphosphinin-2-yl]-(aryl/alkyl)methanols (7a-k) based on the pudovick reaction was accomplished in the presence of niobium pentoxide (nb(2)o(5)) without using an external chiral ligand. nb(2)o(5) appears to form the metal complex intermediate catalyst system (6) by reacting with 3-(3-chloro-4-fluoro-phenyl)-3,4-dihydrobenzo[e][1,3,2]oxazaphosphinine-2-oxide (4), which not only directs ...200717661484
complete genome sequences of maize dwarf mosaic and sugarcane mosaic virus isolates coinfecting maize in spain.the genomes of spanish isolates of maize dwarf mosaic virus (mdmv-sp) and sugarcane mosaic virus (scmv-sp) were completely sequenced. nucleotide sequence identities of scmv-sp to those of other scmv isolates ranged from 79 to 90%. mdmv-sp shared 85% nucleotide identity with the only other fully sequenced isolate of mdmv. mdmv-sp and scmv-sp differed from each other by 31% in their nucleotide sequences. phylogenetic analyses showed that scmv isolates group by host rather than by geographical loca ...200717680319
a characterization of a mutm/fpg ortholog in sugarcane--a monocot plant.plant genomic projects, such as arabidopsis thaliana, rice, and maize, have provided excellent tools for comparative genome analysis on base excision dna repair (ber). a data-mining study associated with the sucest genome project identified two est clusters that shared homology to the bacteria mutm/fpg protein. comparative analyses presented here show a duplication of the mutm/fpg gene in sugarcane, wheat and rice. the complementation assays show that both cdnas from sugarcane are able to comple ...200717686457
heterologous expression of new antifungal chitinase from wheat.chitinases (ec 3.2.1.14) have been grouped into seven classes (class i-vii) on the basis of their structural properties. chitinases expressed during plant-microbe interaction are involved in defense responses of host plant against pathogens. in the present investigation, chitinase gene from wheat has been subcloned and overexpressed in escherichia coli bl-21 (de3). molecular phylogeny analyses of wheat chitinase indicated that it belongs to an acidic form of class vii chitinase (glycosyl hydrola ...200717697785
[trapping techniques for solenopsis invicta].a field study was made to investigate the trapping effects of different attractants, traps, and wind directions on solenopsis invicta. the results showed that among the test attractants, tb1 (50 g fishmeal, 40 g peptone, 10 ml 10% sucrose water solution and 20 ml soybean oil) had the best effect, followed by tb2 (ham), tb6 (100 g cornmeal and 20 ml soybean oil) and tb4 (10 ml 10% sucrose water solution, 100 g sugarcane powder and 20 ml soybean oil), with a mean capture efficiency being 77.6, 58. ...200717763750
inhibition of agrobacterium-induced cell death by antiapoptotic gene expression leads to very high transformation efficiency of banana.the death of plant cells in culture following exposure to agrobacterium tumefaciens remains a major obstacle in developing agrobacterium-mediated transformation into a highly efficient genotype-independent technology. here, we present evidence that a. tumefaciens exposure induces cell death in banana cell suspensions. more than 90% of embryogenic banana cells died after exposure to a. tumefaciens and cell death was accompanied by a subset of features associated with apoptosis in mammalian cells, ...200717849707
a two-dimensional proteome reference map of herbaspirillum seropedicae proteins.herbaspirillum seropedicae is an endophytic diazotroph associated with economically important crops such as rice, sugarcane, and wheat. here, we present a 2-d reference map for h. seropedicae. using maldi-tof-ms we identified 205 spots representing 173 different proteins with a calculated average of 1.18 proteins/gene. seventeen hypothetical or conserved hypothetical orfs were shown to code for true gene products. these data will support the genome annotation process and provide a basis on which ...200717853511
diversity of cultivated endophytic bacteria from sugarcane: genetic and biochemical characterization of burkholderia cepacia complex isolates.bacteria were isolated from the rhizosphere and from inside the roots and stems of sugarcane plants grown in the field in brazil. endophytic bacteria were found in both the roots and the stems of sugarcane plants, with a significantly higher density in the roots. many of the cultivated endophytic bacteria were shown to produce the plant growth hormone indoleacetic acid, and this trait was more frequently found among bacteria from the stem. 16s rrna gene sequence analysis revealed that the select ...200717905875
influence of vermicomposting on solid wastes decomposition kinetics in soils.the effect of vermicomposting on kinetic behavior of the products is not well recognized. an incubation study was conducted to investigate c mineralization kinetics of cow manure, sugarcane filter cake and their vermicomposts. two different soils were treated with the four solid wastes at a rate of 0.5 g solid waste c per kg soil with three replications. soils were incubated for 56 d. the co(2)-c respired was monitored periodically and a first-order kinetic model was used to calculate the kineti ...200717910115
d-003 does not possess oestrogenic potential in-vivo: findings of the uterotrophic assay.d-003 is a mixture of long-chain fatty acids purified from sugarcane wax that inhibits both cholesterol synthesis prior to mevalonate formation, and lipid peroxidation. d-003 has been shown to prevent bone loss and bone resorption in ovariectomized rats, and significantly improves bone resorption markers in postmenopausal women with reduced bone mineral density. as hormone-replacement therapy, d-003 displays cholesterol-lowering and anti-resorptive effects. we have studied its potential oestroge ...200717910820
f2 screen for resistance to a bacillus thuringiensis-maize hybrid in the sugarcane borer (lepidoptera: crambidae).a novel f2 screening technique was developed for detecting resistance in sugarcane borer, diatraea saccharalis (f.), to transgenic bacillus thuringiensis (bt)-maize expressing the cry1ab insecticidal protein. the f2 screening method involved (i) collecting larvae from maize fields; (ii) establishing two-parent families; (iii) screening f2 neonates for survival on bt-maize leaf tissues; and (iv) confirming resistance on commercial bt-maize plants. with the f2 screening method, 213 iso-line famili ...200717916262
effects of dietary fibers on weight gain, carbohydrate metabolism, and gastric ghrelin gene expression in mice fed a high-fat diet.diets that are high in dietary fiber are reported to have substantial health benefits. we sought to compare the metabolic effects of 3 types of dietary fibers -- sugarcane fiber (scf), psyllium (psy), and cellulose (cel) -- on body weight, carbohydrate metabolism, and stomach ghrelin gene expression in a high-fat diet-fed mouse model. thirty-six male mice (c57bl/6) were randomly divided into 4 groups that consumed high-fat diet alone (hfd) or high-fat diet containing 10% scf, psy, and cel, respe ...200717998014
cell immobilization and xylitol production using sugarcane bagasse as raw material.sugarcane bagasse pretreated by three different procedures (with 2% [v/v] polyethyleneimine (pei), with 2% [w/v] naoh, or with a sequence of naoh and pei) was used as cell immobilization carrier for xylitol production by candida guilliermondii yeast. fermentations using these pretreated carriers were performed in semidefined medium and in a hydrolysate medium produced from sugarcane bagasse hemicellulose. sugarcane bagasse pretreated with naoh was the best carrier obtained with respect to immobi ...200718025553
prospecting the utility of a pmi/mannose selection system for the recovery of transgenic sugarcane (saccharum spp. hybrid) plants.for the first time, the phosphomannose isomerase (pmi, ec 5.3.1.8)/mannose-based "positive" selection system has been used to obtain genetically engineered sugarcane (saccharum spp. hybrid var. cp72-2086) plants. transgenic lines of sugarcane were obtained following biolistic transformation of embryogenic callus with an untranslatable sugarcane mosaic virus (scmv) strain e coat protein (cp) gene and the escherichia coli pmi gene mana, as the selectable marker gene. postbombardment, transgenic ca ...200717149641
heavy metal concentrations in soils and plant accumulation in a restored manganese mineland in guangxi, south china.heavy metal contamination of metal-mined soils is a widespread problem in china. in the restored (over 20 years) lipu manganese mineland, 36 plant species from 22 families were found colonizing, some of which were planted agronomic ones. heavy metal concentrations in tailings were very high. minesoils were basically unpolluted, but soils in the remaining mining area and in the vicinity of tailings dumps were polluted by cd and mn. measurements of metal contents in dominant plants showed they wer ...200717014941
evidence for the dispersal of a unique lineage from asia to america and africa in the sugarcane fungal pathogen ustilago scitaminea.the basidiomycete ustilago scitaminea sydow, which causes sugarcane smut disease, has been spreading throughout africa and america since the 1940s. the genetic diversity and structure of different populations of this fungus worldwide was investigated using microsatellites. a total of 142 single-teliospore were isolated from 77 distinct whips (sori) collected in 15 countries worldwide. mycelium culture derived from on generation of selfing of these single teliospores were analysed for their polym ...200716979360
production of cellulase-free endoxylanase from novel alkalophilic thermotolerent bacillus pumilus by solid-state fermentation and its application in wastepaper recycling.the present study aimed at optimization of culture condition for the enhanced production of extra cellular thermostable cellulase-free xylanase from bacillus pumilus by solid-state fermentation. batch studies were carried out to evaluate various agro-industrial residues such as rice bran, rice husk, rice straw, sawdust, coconut pith, sugarcane bagasse and wheat bran for enzyme production by the bacterial culture. the endoxylanase production was highest on wheat bran media (5582 u/gds), which was ...200716844369
arabidopsis sucrose transporter atsuc9. high-affinity transport activity, intragenic control of expression, and early flowering mutant phenotype.atsuc9 (at5g06170), a sucrose (suc) transporter from arabidopsis (arabidopsis thaliana) l. heynh., was expressed in xenopus (xenopus laevis) oocytes, and transport activity was analyzed. compared to all other suc transporters, atsuc9 had an ultrahigh affinity for suc (k(0.5) = 0.066 +/- 0.025 mm). atsuc9 showed low substrate specificity, similar to atsuc2 (at1g22710), and transported a wide range of glucosides, including helicin, salicin, arbutin, maltose, fraxin, esculin, turanose, and alpha-me ...200717098854
immunostimulating effects of the polyphenol-rich fraction of sugar cane (saccharum officinarum l.) extract in chickens.the phagocytic activity of peripheral blood leukocytes (pbl) in chickens orally administered sugar cane extracts (sce) or polyphenol-rich fraction (prf) of sce (500 mg/kg/day) for 3 consecutive days increased significantly, when compared with that of saline-administered control chickens. chickens orally administered sce or prf (500 mg/kg/day) for 3 consecutive days showed significantly higher antibody responses against sheep red blood cells and brucella abortus than control chickens. in addition ...200717117449
domestication of maize, sorghum, and sugarcane did not drive the divergence of their smut pathogens.we investigated two alternative hypotheses for the origin of crop pathogen species: that human-mediated agricultural practices drove the divergence of many crop plant pathogen species or that coevolutionary processes in natural populations of the crops' ancestors drove divergence of pathogen species. we distinguished between these two hypotheses by constructing a robust multigene phylogeny and estimating the dates of divergence among four, monophyletic species of smut fungi (ustilago maydis, u. ...200717348948
antiproliferative and antioxidant activities of a tricin acylated glycoside from sugarcane (saccharum officinarum) juice.from sugarcane juice, a flavone, identified by spectroscopic methods as tricin-7-o-beta-(6''-methoxycinnamic)-glucoside, was isolated, in addition to orientin. the tricin derivative was shown to have antioxidant activity higher than trolox by means of the dpph assay and lower by the beta-carotene/linoleic acid system. it showed in vitro antiproliferative activity against several human cancer cell lines, with higher selectivity toward cells of the breast resistant nic/adr line.200717350657
signal transduction-related responses to phytohormones and environmental challenges in sugarcane.sugarcane is an increasingly economically and environmentally important c4 grass, used for the production of sugar and bioethanol, a low-carbon emission fuel. sugarcane originated from crosses of saccharum species and is noted for its unique capacity to accumulate high amounts of sucrose in its stems. environmental stresses limit enormously sugarcane productivity worldwide. to investigate transcriptome changes in response to environmental inputs that alter yield we used cdna microarrays to profi ...200717355627
sugarcane borer (lepidoptera: crambidae) resistance to transgenic bacillus thuringiensis maize.transgenic maize, zea mays l., expressing the bacillus thuringiensis (bt) crylab toxin has been planted to extensive areas across the united states and several other countries, but no resistance has been documented in field populations oflepidopteran target pests. this article describes the first report of resistance alleles to commercially available cry1ab bt maize in a louisiana population of sugarcane borer, diatraea saccharalis (f.) (lepidoptera: crambidae). two hundred thirteen two-parent i ...200717370824
how safe is sugarcane juice? 200717377362
enumeration, isolation and identification of diazotrophs from korean wetland rice varieties grown with long-term application of n and compost and their short-term inoculation effect on rice plants.this study has been aimed (i) to isolate and identify diazotrophs from korean rice varieties; (ii) to examine the long-term effect of n and compost on the population dynamics of diazotrophs and (iii) to realize the shot-term inoculation effect of these diazotrophs on rice seedlings.200717381741
purification and characterization of two sugarcane bagasse-absorbable thermophilic xylanases from the mesophilic cellulomonas flavigena.we report the purification and characterization of two thermophilic xylanases from the mesophilic bacteria cellulomonas flavigena grown on sugarcane bagasse (scb) as the only carbon source. extracellular xylanase activity produced by c. flavigena was found both free in the culture supernatant and associated with residual scb. to identify some of the molecules responsible for the xylanase activity in the substrate-bound fraction, residual scb was treated with 3 m guanidine hydrochloride and then ...200717219190
effects of a mixture of fatty acids from sugar cane (saccharum officinarum l.) wax oil in two models of inflammation: zymosan-induced arthritis and mice tail test of psoriasis.a mixture of fatty acids obtained from sugar cane (saccharum officinarum l.) wax oil (fam), in which the main constituents are palmitic, oleic, linoleic, and linolenic acids, was evaluated in two models of inflammation: zymosan-induced arthritis and in the tail test for psoriasis, both on mice. in the first model, fam significantly reduced zymozan-induced increase of beta glucuronidase (de(50) 90+/-7 mg/kg). histopathological studies showed inhibition in cellular infiltration and reduction of sy ...200717292594
comparison of composition and absorption of sugarcane policosanols.policosanols (pc) exist as very-long-chain alcohols derived from sugarcane currently used in many countries as a cholesterol-lowering therapy. pc purity and relative percentage composition have been suggested as primary reasons why the original cuban pc (opc) supplements possess lipid-lowering efficacy. the purpose of the present study was, first, to compare the relative percentage purity and pc composition of both opc and alternative sources of pc (apc). a second objective was to feed syrian ha ...200717298709
growth and metabolism in sugarcane are altered by the creation of a new hexose-phosphate sink.an efficient in planta sugarcane-based production system may be realized by coupling the synthesis of alternative products to the metabolic intermediates of sucrose metabolism, thus taking advantage of the sucrose-producing capability of the plant. this was evaluated by synthesizing sorbitol in sugarcane (saccharum hybrids) using the malus domestica sorbitol-6-phosphate dehydrogenase gene (mds6pdh). mature transgenic sugarcane plants were compared with untransformed sugarcane variety q117 by eva ...200717309679
heterologous signals allow efficient targeting of a nuclear-encoded fusion protein to plastids and endoplasmic reticulum in diverse plant species.approximately 30% of plant nuclear genes appear to encode proteins targeted to the plastids or endoplasmic reticulum (er). the signals that direct proteins into these compartments are diverse in sequence, but, on the basis of a limited number of tests in heterologous systems, they appear to be functionally conserved across species. to further test the generality of this conclusion, we tested the ability of two plastid transit peptides and an er signal peptide to target green fluorescent protein ...200717309684
doubled sugar content in sugarcane plants modified to produce a sucrose isomer.sucrose is the feedstock for more than half of the world's fuel ethanol production and a major human food. it is harvested primarily from sugarcane and beet. despite attempts through conventional and molecular breeding, the stored sugar concentration in elite sugarcane cultivars has not been increased for several decades. recently, genes have been cloned for bacterial isomerase enzymes that convert sucrose into sugars which are not metabolized by plants, but which are digested by humans, with he ...200717207261
production of polyhydroxybutyrate in sugarcane.we report here the production of the bacterial polyester, polyhydroxybutyrate (phb), in the crop species sugarcane (saccharum spp. hybrids). the phb biosynthesis enzymes of ralstonia eutropha [beta-ketothiolase (phaa), acetoacetyl-reductase (phab) and phb synthase (phac)] were expressed in the cytosol or targeted to mitochondria or plastids. phb accumulated in cytosolic lines at trace amounts, but was not detected in mitochondrial lines. in plastidic lines, phb accumulated in leaves to a maximum ...200717207265
members of the ethylene signalling pathway are regulated in sugarcane during the association with nitrogen-fixing endophytic bacteria.nitrogen-fixing bacteria have been isolated from sugarcane in an endophytic and beneficial interaction that promotes plant growth. in this work, for the first time, the involvement of ethylene signalling in this interaction was investigated by molecular characterizing members of this pathway in sugarcane. the expression pattern of a putative ethylene receptor (scer1) and two putative erf transcription factors (scerf1 and scerf2) show exclusive modulation in plants inoculated with the diazotrophi ...200717210990
isolation and characterization of xylitol-producing yeasts from the gut of colleopteran insects.a total of 35 yeasts were isolated from the gut of beetles collected from hyderabad city, india. twenty of these yeasts utilized xylose as a sole carbon source but only 12 of these converted xylose to xylitol. the ability to convert xylose to xylitol varied among the isolates and ranged from 0.12 to 0.58 g/g xylose. based on the phenotypic characteristics and phylogenetic analysis of the d1/d2 domain sequence of 26s rrna gene, these isolates were identified as members of pichia, candida, issatch ...200717710483
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