Publications

non-equivalence for bean seeds of clockwise and counterclockwise magnetic motion: a novel terrestrial adaptation?	1. three experiments on water-uptake in pinto beans, phaseolus vulgaris, during their first four hours in water have all indicated that clockwise and counterclockwise rotating magnetic fields have statistically significantly different effects upon the rate of the process. 2. the results suggest that this difference is independent of time and place. critically performed experiments at evanston, illinois and woods hole, massachusetts, over more than a two-year span, all gave essentially the same r ...	1975	29324024
foliar iron spray potentiates growth of seedlings on iron-free media.	growth of bean (phaseolus vulgaris cv. ;brittle wax') seedlings in iron-free media caused large reductions in root size and dye reduction capacity. shoot growth was also severely retarded and the classical symptoms of chlorosis were observed. a single prophylactic spray of feso(4), applied to the primary leaves of 7-day seedlings, enabled subsequent growth to equal that obtained when iron was continuously supplied to the roots over a 12-day period, although chlorophyll levels were lower. by addi ...	1975	16659232
light sensitivity of na, rb, and k absorption by different tissues of bean leaves.	autoradiographs of (22)na-loaded bean (phaseolus vulgaris l. cv. ;brittle wax') leaf slices showed that most of the tracer was concentrated in vascular tissue. rubidium-86 was uniformly distributed in slices that had been incubated in darkness; after incubation in the light many small dark spots appeared on the autoradiographs, apparently corresponding with the stomata. autoradiographs of (42)k-loaded slices showed a rather uniform distribution of the tracer, whether the slices had been incubate ...	1975	16659230
plastid development in primary leaves of phaseolus vulgaris. development of plastid adenosine triphosphatase activity during greening.	the etioplasts of dark-grown bean leaves showed atpase (adenosine triphosphatase) activity which had a ph optimum of 8.5, was stimulated by dithiothreitol and unaffected by light-triggering. bean chloroplasts showed a low activity of dark-induced atpase with a ph optimum of 8.5 and a substantial amount of light-triggered activity with a ph optimum of 8.0. the light-triggered activity depended on dithiothreitol and mg2+ and was promoted by phenazine methosulphate. light-triggered atpase activity ...	1975	990
effects of plant species, age and part on the disappearance of sevin, nuvacron and malathion residues.	residues as determined by bioassay using daphnia or mosquito larvae were in agreement with each other in most cases except sevin residues at 1 h and 8 days after treatment of mallow. the mosquito larvae failed to record nuvacron, sevin and malathion on 45-day-old plants on the 8th, 12th and 24th day, respectively, whereas residues on younger plants continued to affect mosquitoes up to the 12th day and disappeared only on the 24th day. daphnia continued to show toxicity up to the 24th day on youn ...	1975	807993
collaborative study of the 2,4-dinitrophenyl ether multiresidue method for use in determining four carbamate pesticides.	eight laboratories tested the 2,4-dinitrophenyl ether method for determining carbamate pesticide residues by gas-liquid chromatography. propoxur, carbofuran, carbanolate, and carbaryl in apples, corn, green beans, and a leafy vegetable (kale, collards, cabbage, or turnip greens) were determined at the fortification levels of 0.1, 0.2, and 0.5 ppm. average recoveries of propoxur were 87-95%; carbofuran, 87-100%; carbanolate, 89-102%; and carbaryl, 77-92%. no serious background problems were encou ...	1975	806576
[soaking temperature and enzyme activation in seeds of phaseolus vulgaris l].		1975	1203121
zinc nutrition and starch metabolism in phaseolus vulgaris l.	the effect of zn nutrition on leaf starch metabolism was studied in two navy bean (phaseolus vulgaris l.) varieties, sanilac and saginaw. sanilac is much more susceptible to zn deficiency than is saginaw. the variables examined in these two strains were starch content, the activity of soluble starch synthetase (adp-glucose: starch alpha-4-glucosyltransferase, ec 2.4.1.b), and the size and number of starch grains. all of these variables decreased during zn deficiency. the reductions were much gre ...	1975	16659093
effects of heat shock on growth and on lipid and beta-glucan synthetases in leaves of phaseolus vulgaris and vigna sinensis.	primary leaves of intact plants of phaseolus vulgaris and vigna sinensis 7 to 9 days old were dipped in water for 2 minutes at 47.4 +/- 0.2 c and 48.7 +/- 0.5 c, respectively. leaf growth was inhibited within the first 20 hours, and growth partially or completely recovered in a subsequent 20-hour period. analysis of the glucolipid and beta-1, 3- and beta-1, 4-glucan synthetases showed that the most evident effects of heat shock were the severe inhibition of the synthesis of beta-1, 4-glucosyl gl ...	1975	16659076
proceedings: behaviour of cotyledons in decapitated seedlings of phaseolus vulgaris l. cv. limburg.		1975	50793
is p-protein actin-like?-not yet.	microfilaments associated with cytoplasmic streaming in nitella flexilis internodes can be decorated with heavy meromyosin (hmm) from rabbit, both in vitro in cytoplasmic suspensions, and in situ in glycerinated cell segments. the bound hmm consists of clearly discernible, polarized arrowheads in a regular repeat of 360-380 å that are similar to those produced on f-actin. in contrast, similar arrowheads or decorations are not evident on p-protein filaments in sieve elements of glycerinated hypoc ...	1975	24435439
stomatal closure in response to xanthoxin and abscisic acid.	the stomata of detached leaves of commelina communis l., hordeum vulgare l., zea mays l., vicia faba l., phaseolus vulgaris l. and xanthium strumarium l. closed when xanthoxin (xan) was added to the transpiration stream. xan was approximately half as active as (+)-abscisic acid (aba) at an equivalent concentration. xan, like aba, sensitized stomata of xanthium strumarium to co2. in contrast to aba, xan was ineffective in closing stomata of isolated epidermal strips of c. communis or v. faba. thi ...	1975	24435339
the occurrence and ultrastructure of chloroplasts in the phloem parenchyma of leaves of c4 dicotyledons.	chloroplasts were found to be common within the phloem parenchyma of leaves of dicotyledons, although they were not observable, at the light microscope level, in the vascular cells of any grasses examined. the phloem-cell chloroplasts of boerhaavia paniculata l. and portulaca oleracea l. (both c4 plants) were structurally similar to each other, even though the chloroplasts of their surrounding bundle sheaths were of different types. they were also similar to the phloem-cell chloroplasts of phase ...	1975	24435124
photosynthesis and the induction of nitrate reductase and nitrite reductase in bean leaves.	in laaves of phaseolus vulgaris l. cv. prelude, the light-induced increase in activity of nadh-nitrate oxidoreductase (e.c.1.6.6.2; nar) and reduced benzylviologennitrite oxidoreductase (e.c.1.6.6.4; nir) starts at a certain stage in the development of the chloroplasts. in leaves with completely developed chloroplasts, a higher increase in activity of nar and nir is observed, after induction by the addition of nitrate, in the light than in the dark. dcmu inhibits the increase in activity of the ...	1975	24435084
correlative inhibition of lateral bud growth in phaseolus vulgaris l. timing of bud growth following decapitation.	on intact, 3-week-old plants of phaseolus the larger bud in the axils of the primary leaves shows slow, continuous elongation growth. release from correlative inhibition can be detected within 30 min following decapitation. when 0.1% indoleacetic acid in lanolin is applied to the decapitated stem stump, the lateral bud shows slow growth during the first 7 h, then stops completely for a further 15 h but after 2 days a further gradual increase in length is observed.the movement of (14)c-labelled a ...	1975	24435080
the induction of transport channels by auxin.	this work deals with measurements of the transport of radio-activity from [(3)h] indole acetic acid during early stages of auxin-induced vascular differentiation. transverse wounds were made in stems of phaseolus vulgaris l. and the radioactivity which was transported around these wounds was measured. treatments with unlabelled auxin above the wounds increased transport within 16 hours of the time they were first applied while the maturation of vascular elements in response to these treatments s ...	1975	24430469
plant cell-wall microfibril disposition revealed by freeze-fractured plasmalemma not treated with glycerol.	the plasmalemmata of root tip cells of phaseolus vulgaris l. appear to be well preserved in freeze-fractured material in which no glycerol pre-treatment has been used. the plasmalemmata retain the impressions made by the microfibrils and pit-fields of the cell walls against which these membranes are pressed in turgid cells. material freeze-fractured in this way is therefore more suitable than glycerol-treated material (in which these impressions are not retained) for the study of membrane featur ...	1975	24430091
an ultrastructural study of acid phosphatase localization in cells of phaseolus vulgaris phloem by the use of the azo dye method.	the localization of acid phosphatase was studied in the sieve elements and companion cells in the phloem tissue of the bean, phaseolus vulgaris l. the various organelles in the two kinds of cell showed fine granules of the azo dye as the final reaction product. the aggregated smooth endoplasmic reticulum displayed the dye particularly consistently. the dye was also present in the plasmodesmata and in the contents of the sieve plate pores. the reaction product was conspicuous in the cell walls an ...	1975	1209585
partial purification of adenosine 3':5'-clclic monophosphate phosphodiesterase from phaseolus vulgaris l.: associated activator and inhibitors.		1975	165999
cell-free synthesis of the major storage protein of the bean, phaseolus vulgaris l.	as seeds of the french bean (phaseolus vulgaris, l. cv. tendergreen) mature, a single protein, g1 globulin (analogous to legumin), represents the majority of protein synthesized. washed polysomes extracted from developing cotyledons had little endogenous activity in amino acid incorporation, but on addition of cell-free extracts from wheat germ, active incorporation was obtained, the level being similar to that with viral rna as messenger. the mg(2+) optimum for protein synthesis in the presence ...	1975	16659394
heritable variation in a polypeptide subunit of the major storage protein of the bean, phaseolus vulgaris l.	electrophoretic analysis of the major seed protein, g1 globulin, from four strains of phaseolus vulgaris l. revealed a three-banded pattern for two strains having a high methionine content (bbl 240 and pi 302,542). the other two strains (pi 207,227 and pi 229,815) known to have a lower seed methionine content, had a two-banded subunit pattern for the g1 globulin. analytical ultracentrifugation confirmed that globulin from the two-banded strains underwent ph-dependent reversible dissociation simi ...	1975	16659393
formation of alpha-amylase and endogenous gibberellins in cotyledons of phaseolus vulgaris.		1975	58599
an ultrastructural study of acid phosphatase localization in phaseolus vulgaris xylem by the use of an azo-dye method.	the localization of acid phosphatase during xylem development has been examined in the bean, phaseolus vulgaris. the azo dye, the final reaction product, is initially prominent in the dictyosomes, vesicles apparently participating in secondary wall formation, and in the middle lamella of the young vessel element. final reaction particles are also present in mitochondria, chloroplasts, and certain vacuoles and are sparsely scattered in the cytoplasm. at a later stage of vessel differentiation, th ...	1975	54361
factors affecting the molecular structure and the agglutinating ability of concanavalin a and other lectins.	ultracentrifugation analyses were performed on lectins under varying conditions of ph, ionic strength and temperature. it has been demonstrated that the phytohemagglutinin from phaseolus vulgaris, the wheat germ agglutinin and the soybean agglutinin are stable when these parameters are varied, whereas the concanavalin a molecule exhibits a striking reversible dimer-tetramer transition with variation in ph (from 6.0 to 7.2) and temperature (from 4 degrees up to 37 degrees c). it has also been dem ...	1975	1259
effects of abscisic acid on growth, rna metabolism, and respiration in germinating bean axes.	the effect of abscisic acid on growth, respiration, the atp pool, and rate and amount of rna synthesis in aseptically cultured axes of phaseolus vulgaris during the first 24 hours of germination has been measured in experiments where the duration of abscisic acid application and its concentration have been varied. at concentrations from 10(-7) to 10(-4)m, abscisic acid inhibits synthesis of rna with maximal inhibition (80%) at 10(-5)m. rna synthesis is inhibited by abscisic acid at all times exa ...	1975	16659346
sh-proteinase from bean phaseolus vulgaris var. perlicka.	an sh-proteinase (ec 3.4.22.-) has been isolated from beans of the species phaseolus vulgaris var. perlicka. the enzyme is homogeneous when subjected to disc electrophoresis, electrofocusing and sedimentation analysis. the molecular weight was determined as 26,000-28,000 by gel filtration, 30,850 +/- 1500 by sedimentation analysis and 26,930-27,410 by calculation from the amino acid composition (lys20-21, his3, arg9, asp21-22, thr13, ser18, pro12-13, glu23-24, gly30, ala16, cys/29, val19, met1, ...	1975	241406
in vitro simulation of cytoplasmic membrane senescence in cotyledons.	the loss of microsomal nadh-cytochrome c reductase activity (ec 1.6.99.3) in cotyledons, known to accompany germination of phaseolus vulgaris and thought to reflect the progress of cytoplasmic membrane senescence, can be simulated in an in vitro system in which isolated microsomes from 2-day-old tissue are treated with cytosol fractions (microsomal supernatants). inactivation of the enzyme is comparatively low when the microsomes are treated for 4 hours with cytosol fractions from 1- and 2-day-o ...	1975	16659335
accumulation of delta-aminolevulinic acid and its relation to chlorophyll synthesis and development of plastid structure in greening leaves.	levulinic acid inhibited the greening of etiolated maize (zea mays) and bean (phaseolus vulgaris) leaves and caused accumulation of delta-aminolevulinic acid (ala). ala accumulation in maize was equivalent to the decrease in chlorophyll, over a wide range of experimental conditions. it was saturated at low light intensities and was not limited by the supply of substrates during the early hours of greening. during 20 hours in light, levulinic acid had little effect on the structural development o ...	1975	16659331
localization of lectins in legume cotyledons.	high-resolution techniques for the localization of lectins are described. concanavalin a (con a) and phytohaemagglutinin (pha) are localized using a fluorescent method with (fitc)-labelled immunoglobulins which bind to the lectins in sections of jack and red kidney bean cotyledons. specificity is defined by the use of specific sugar inhibitors. both con a and pha are found in cytoplasmic sites. lectins with beta-glycoside specificity are detected with red-coloured artificial carbohydrate antigen ...	1975	1176546
purification of the phytohemagglutinin family of proteins from red kidney beans (phaseolus vulgaris) by affinity chromatography.	half-gram quantities of phytohemagglutinin lectins are purified from saline extracts of red kidney beans (phaseolus vulgaris) by affinity absorption on porcine thyroglobulin-sepharose. all of the mitogenic and erythroagglutinin activity of the saline extract is removed by this absorbent, and 74% of the original erythroagglutinating activity elutes from the affinity absorbent representing a 25-fold purification. five distinct proteins appear in the polyacrylamide gel electrophoresis of the affini ...	1975	1174570
the effect of carbohydrates and arginine on arginine metabolism by excised bean leaves in the dark.	the effect of carbohydrate on arginine utilization by excised bean (phaseolus vulgaris l. var. tendergreen) leaves in the dark was studied by adding arginine to leaves differing in carbohydrate levels, and measuring the arginine content of the leaves at intervals. in nonstarved leaves, the arginine content decreased steadily after vacuum infiltration of 10 mm arginine and was essentially completely utilized by 36 hours after infiltration. in starved leaves, the arginine content did not decrease ...	1975	16659159
extensive homology between the subunits of the phytohemagglutinin mitogenic proteins derived from phaseolus vulgaris.	the phytohemagglutinin mitogenic proteins derived from phaseolus vulgaris comprise a class of five glycoproteins that are isomeric tetramers composed of varying proportions of two different subunits (l and r). within the native tetramer, the l subunit is a potent leukoagglutinin and mitogen that lacks hemagglutinating properties, whereas the r subunit is a potent hemagglutinin with little or no mitogenic activity. the subunits have been isolated in homogeneous form by isoelectric focusing in 8 m ...	1975	1055414
precipitation reactions of red kidney bean (phaseolus vulgaris) phytohemagglutinin isolectins.		1976	824093
[rheology and spinning of alkaline solution of field bean protein and casein].	from field bean protein and a mixture of field bean protein with casein to equal parts are prepared with sodium hydroxide high protein-containing alkaline solutions, which show pseudoplastic flow. the flow curves of the pseudoplastic field bean protein-casein (i:i)-solutions are described mathematically with the ostwaldian power statement. in a suitable scope of spinning of this solutions are carried out complete factorial experiments, which guide to regression equations of lg k and n. g k depen ...	1976	8719
light-sensitive inhibitors of alpha-amylase synthesis in cotyledons of phaseolus vulgaris l.		1976	71076
changing ratios of phototransformable protochlorophyll and protochlorophyllide of bean seedlings developing in the dark.	protochlorophyll (pchl) and protochlorophyllide (pchlide) are at comparable levels in 2-day-old (young) etiolated bean leaves (phaseolus vulgaris l. var. red kidney). during subsequent development in the dark, both pigments increase, but the rate of pchlide increase is greater than that of pchl, leading to the commonly observed predominance of pchlide beyond 7 days (old leaves). both protopigments are phototransformable to their respective chlorophyll(ide) photoproducts throughout dark developme ...	1976	16659485
effects of carbon monoxide and nitrogen dioxide on garden pea and string bean.		1976	1252637
proceedings: light controlled regulation of alpha-and beta-amylase activity in cotyledons of phaseolus vulgaris.		1976	60966
proceedings: relationships between epicotyl growth and endogenous iaa-content in etiolated seedlings of phaseolus vulgaris l. cv. limburg.		1976	60933
correlation of the induction of various enzymes concerned with phenylpropanoid and lignin synthesis during differentiation of bean callus (phaseolus vulgaris l.).	the activities of phenylalanine ammonia lyase and caffeate-0-methyl transferase in bean callus tissue, during differentiation, were induced in a coordinated manner over a period of 21 days. neither enzyme activity could be induced to increase when differentiation was repressed by the addition of abscisic acid to the induction medium. the formation of soluble phenols was also increased during the induction of differentiation but it was also increased by the transfer to induction medium of an old ...	1976	24430755
conversion of photosynthetic products in the light in co2-free o 2 and n 2 in leaves of zea mays l. and phaseolus vulgaris l.	after 10 min illumination of segments of bean (phaseolus vulgaris l.) or maize (zea mays l.) leaves in air with (14)co2, the atmosphere was changed to co2-free o2 or n2 and conversion of photosynthetic products in the light was investigated. the experiments have shown that after the (14)co2 assimilation period the bean leaves contain the pool of weakly fixed (14)c (wf-(14)c) which is converted into stable products during the subsequent period of illumination in co2-free n2. in o2 atmosphere the ...	1976	24424758
a scanning electron microscopic study of trichomes of iaa-induced tumours in bean embryos (phaseolus vulgaris l.).	a scanning electron microscopic study of trichome morphology of bean embryo, growing in semi-solid synthetic medium, has been undertaken. the work shows some distinct differences in trichome morphology as well as variation in their development, due to the effect of indole-3-acetic acid (iaa).	1976	1027550
trypsin isoinhibitors from garden beans (phaseolus vulgaris).		1976	1012025
absorption and metabolism of a selective insecticide, 2,2-dimethyl-2,3-dihydrobenzofuranyl-7 n-dimethoxyphosphinothioyl-n-methylcarbamate, in bean plants.	the absorption and metabolism of 2,2-dimethyl-2,3-dihydrobenzofuranyl-7 n-dimethoxyphosphinothioyl-n-methylcarbamate (psc), a selective, insecticidally active carbofuran derivative, was studied in red kidney bean plants. psc was absorbed from hydroponic culture media and was translocated throughout the plants. psc was metabolized to carbofuran, 3-hydroxycarbofuran, and 3-ketocarbofuran and these materials accounted for 25% of the recovered radioactivity. each of these compounds are more toxic to ...	1976	999337
synthesis of o-beta-d-galactopyranosyl-(1 linked to 4)-o-(2acetamido-2-deoxy-beta-d-glucopyranosyl)-(1 linked to 2)-d-mannose and its interaction with various lectins.	2-methyl-[3,6-di-o-acetyl-2-deoxy-4-o-(2,3,4,6-tetra-o-acetyl-beta-d-galactopyranosyl)-alpha d-glucopyrano]-[2,1-d]-2oxazoline (4) was prepared from 2-acetamido-3,6-di-o-acetyl-2-deoxy-4-o-(2,3,4,6-tetra-o-acetyl-beta-d-galactopyranosyl)-alpha-d-glucopyranosyl chloride. condensation of 3,4:5,6-di-o-isopropylidene-d-mannose dimethyl acetal with 4 in the presence of a catalytic amount of p-toluenesulfonic acid afforded o-(2,3,4,6-tetra-o-acetyl-beta-d-galactopyranosyl)-(1 linked to 4)-o-(2-acetami ...	1976	1016986
identification of a protein factor involved in dithiothreitol activation of nadp malate dehydrogenase from french bean leaves.		1976	12005
pathology and histopathology of pratylenchus scribneri infecting snap bean and lima bean.	the pathological effects of pratylenchus scribneri on susceptible snap bean and resistant lima bean were studied. in a pathogenicity test, the nematode increased nearly 75-fold on snap bean and suppressed top growth. on lima bean, p. scribneri reproduced slowly and did not significantly affect top growth. discreet lesions formed on lima bean roots, but no lesions developed on snap bean roots. paraffin sections taken 2, 5, 13, 25, and 32 days after inoculation showed little cellular necrosis in s ...	1976	19308246
correlation of absorbance changes and thylakoid fusion with the induction of oxygen evolution in bean leaves greened by brief flashes.	dark-grown bean leaves (phaseolus vulgaris) which had been greened for several days in a repetitive series of brief xenon flashes were studied during the initial induction period when o(2) evolution first appears. the induction of o(2) evolution requires actinic irradiation (e.g. 2 mw/cm(2) of red light) and goes to completion in about 8 minutes with a half-time just under 3 minutes. absorbance measurements on the intact leaves showed that a change of a carotenoid pigment, monitored at 505 nm, w ...	1976	16659681
association of latent cellulase activity with plasma membranes from kidney bean abscission zones.	membranes isolated from abscission zones of phaseolus vulgaris l., cv. red kidney, contained cellulase activity. this particulate activity was enhanced 10- to 20-fold by treatment with triton x-100. sucrose density gradient analyses of cell fractions showed that the membranes with which cellulase was associated had a peak equilibrium density of 1.16 to 1.17 g/cm(3) which coincided with that of ion-activated atpase, a marker for plasma membranes. the membrane fraction having the highest cellulase ...	1976	16659672
affinity chromatography of the major seed protein of the bean (phaseolus vulgaris l.).	the major globulin of the french bean (phaseolus vulgaris l.) undergoes a reversible ph-dependent polymerization. at ph values above 6.5, the monomeric form of the protein predominates; and at ph values below 6.5, the protein occurs as a polymer, probably a tetramer. at extremes of ph, the protein dissociates further into peptides. the reversible ph-dependent interaction between globulin subunits is used in this report as the basis for an affinity chromatography procedure for isolation of the gl ...	1976	16659661
growth and development of the american bollworm heliothis armigera hubn. under laboratory mass rearing conditions.	a kidney bean meal diet was the most satisfactory laboratory diet for the larvae of the american bollworm heliothis armigera hubn. optimum rates of survival (63.5%) occurred and the larval growth was better than that of larvae reared on castor oil plant leaves. the pupal weight and the fecundity of the resulting adults were also much better than those on the control host plant.	1976	955000
hooked trichomes: a physical plant barrier to a major agricultural pest.	hooked epidermal appendages (trichomes) on leaves of field bean cultivars effectively capture nymph and adult leafhoppers. frequency of capture and capture mortality are highly correlated with trichome density. hooked trichomes inserted at angles less than 30 degrees are ineffective in capture.	1976	17841820
isolation and analysis of protein bodies from cotyledons of lablab purpureus and phaseolus vulgaris (leguminoseae).	protein bodies, isolated by differential and isopycnic centrifugation, have been observed in transmission and scanning electron microscope and biochemically analysed. the powders of the axes and of the cotyledons contain numerous protein bodies, which in the scanning microscope appear to be surrounded by a more or less torn membrane. the proportion of intact, isolated protein bodies is influenced by the grinding methods, but even in the best conditions soaking disaggregates the majority of them. ...	1976	28869304
enzymic nature of the protein moiety of protochlorophyllide holochrome.	the enzymic nature of the protein moiety of protochlorophyll(ide) holochrome was studied by following the fate of the [(14)c]protochlorophyll(ide) formed when dark-grown barley (hordeum vulgare) or bean (phaseolus vulgaris) leaves are incubated in the dark with 3 mm 4-delta-[(14)c]aminolevulinic acid. it was found that: [list: see text]since turnover of protochlorophyll(ide) was not observed, these results show that there is a free exchange between the old "endogenous" and the new delta-aminolev ...	1976	16659617
a new class of synthetic auxin transport inhibitors.	auxin transport inhibition by a new class of synthetic plant growth regulants, the 2-(3-aryl-5-pyrazolyl)benzoic acids, was examined in bean (phaseolus vulgaris l.) using the donor-receiver agar cylinder technique. these compounds can be prepared by the dehydrogenation and ring cleavage of compounds like dpx-1840 (2-(4-methoxyphenyl)-3,3adihydro-8h-pyrazolo[5,1-a] isoindol-8-one) which was previously reported (plant physiol. 1972. 50: 322-327) to be a potent inhibitor of auxin transport. these n ...	1976	16659581
phytochemical and nutritional studies on pigeon pea and kidney bean cultivated in egypt.	1. the preliminary phytochemical screening of the two seeds established the presence of carbohydrates and/or glycosides, flavnoids, unsaturated sterols and/or triterpenes, saponins, trypsin inhibitors and haemagglutinins. in addition, it established the absence of cardenolides, tannins, alkaloids and oxidase enzyme. 2. certain pharmacopoeial constants, including moisture, ash, acid-insoluble ash, water-soluble ash and crude fibre were determined. 3. the two seeds were subjected to successive ext ...	1976	969710
insecticidal action of the phytohemagglutinin in black beans on a bruchid beetle.	black bean (phaseolus vulgaris) phytohemagglutinin added to the normal diet of a bruchid beetle (callosobruchus maculatus) that can eat phytohemagglutinin-free cowpeas (vigna unguiculata) but not p. vulgaris seeds kills the bruchid larvae. trypsin inhibitors, found in both p. vulgaris and v. unguiculata seeds, have virtually no effect on the bruchid larvae. the conclusion is that a major part of the adaptive significance of phytohemagglutinins in black bean and other legume seeds is to protect t ...	1976	1265481
phytochrome-mediated assembly of polyribosomes in etiolated bean leaves. evidence for post-transciptional regulation of development.	light operating through phytochrome controls the proportion of total ribosomes present as polyribosomes in etiolated leaves of phaseolus vulgaris. similar responses but with slightly different time courses are elicited by brief red light treatment and by continuous far-red or white light. the increase in polyribosome proportions after red light treatment reaches a maximum within 2 h, after which the proportion steadily declines. light treatment appears to lead to increased proportions of polyrib ...	1976	1084278
subunit studies of coupling factor 1 of bean chloroplasts.	a bean chloroplast coupling factor (cf1) with latent ca2+-dependent atpase activity was studied. immunodiffusion of bean (phaseolus vulgaris) chloroplast and etioplast coupling factors and spinach coupling factor against antiserum to spinach coupling factor showed partial identity of the bean coupling factor with that of spinach. an immunoelectrophoretic comparison, under dissociating conditions, of bean leaf extracts and spinach extracts containing cf1 subunits (as determined by sodium dodecyl ...	1976	132266
aging progression involving dwarfism and its acceleration by red light in bean hypocotyls.	the effect of red light on the aging progression of the bean (phaseolus vulgaris l.) hypocotyl segment unit was examined in relation to dwarfism using kentucky wonder (tall) and masterpiece (dwarf) varieties. in both plants, red light promoted the elongation of younger zones and inhibited that of mature zones. the zone exhibiting maximum elongation was shifted to the younger zones by red light irradiation regardless of the plant type, but its extent was greater in the dwarf than in the tall. thu ...	1976	16659523
mesophyll resistance and carboxylase activity: a comparison under water stress conditions.	the response of several leaf gas exchange parameters were monitored with decreasing leaf water potential in phaseolus vulgaris l. leaflets. these included photosynthesis, transpiration, co(2) compensation point, ribulose 1,5-diphosphate carboxylase activity, boundary layer plus stomatal, and mesophyll resistance to diffusion of co(2). mesophyll resistance was calculated under two assumptions: (a) the co(2) concentration at the chloroplast was zero, and (b) it was equal to the co(2) compensation ...	1976	16659506
variants of hamster fibroblasts resistant to ricinus communis toxin (ricin).	1. variant baby-hamster kidney (bhk) cell lines were isolated that grow in the presence of high concentrations of ricin, the toxic lectin of castor beans (ricinus communis). the variant lines were independently derived from several cultures of normal bhk cells which had been exposed to the mutagen, methyl-n-nitro-n-nitrosoguanidine, before selection by ricin. 2. the cell lines maintain a high degree of resistance to ricin after growth in lectin-free medium for prolonged periods and therefore exh ...	1976	1275903
the effects of water stress on abscisic-acid levels and metabolism in roots of phaseolus vulgaris l. and other plants.	abscisic-acid (aba) levels in roots of bean plants exposed to a-4 bar stress in the root medium increased ca. 10fold within 1 h and 16fold by the end of the 2nd h. several types of experiments indicated that there is no transport requirement from the shoot for the increase to occur. aba levels in roots from pea (pisum sativum l.) and sunflower (helianthus annuus l.) also increased in response to a-4 bar stress, although not as dramatically as in bean. when (s)-[2-(14)c]-aba was fed to excised be ...	1976	24424762
effects of ancymidol (a growth retardant) and triarimol (a fungicide) on the growth, sterols, and gibberellins of phaseolus vulgaris (l.).	the effect of the two substituted pyrimidines, ancymidol (a growth retardant) and triarimol (a fungicide) on phaseolus vulgaris was studied. both compounds retarded shoot and root elongation as well as increases in fresh weight. both compounds caused production of ethylene-like responses when given in high dosages or when applied shortly after germination. as growth retardation was shown to occur in the absence of net increase in sterol levels, neither ancymidol nor triarimol apparently retards ...	1976	16659542
[changes in weight of fresh and dried vegetables on cooking].	the present study represents part of a more important work relative to normal food refuse and change of weight of varied food according to different ways of cooking. the study has been realized on vegetables and some fruits of frequent utilization in the population. it was accomplished by 25 voluntary housekeepers from paris and marseille during a whole year according to precise instructions about the method of weighings, every vegetable having to be weighed, three times at different intervals, ...	1976	970838
[chemical modification of proteins. 2. blocking of amino groups and basic amino acids and crosslinking of polypeptide chains in casein and field bean globulin by reaction with dialdehyde starch].	the reaction of dialdehyde starch with casein and field-bean globulin leads to a blocking of the protein amino groups and to a decrease of free lysine, arginine and histidine. maximum values are reached at high protein concentrations and great molar reagent excess. at best 80-93% of the amino groups or of the available lysine may be blocked in this way. for 1% casein and i and 5% globulin solutions, the ph optimum of the reaction lies at approximately 8; for 5% casein solutions, it is shifted to ...	1976	14303
[nutritional value of yellow beans (phaseolus vulgaris) combined with soya (glycine max)].		1976	1050060
[comparative studies on the reactive sites against trypsin of some inhibitors from phaseolus coccineus and phaseolus vulgaris (author's transl)].	three of the trypsin chymotrypsin inhibitors from the seeds of runner beans (phaseolus coccineus l.), pci 3,4(2), and 5, and three of the inhibitors from the seeds of french beans (phaseolus vulgaris var. nanus), pvi 3, 4, and 5, contain a lysine residue in the reactive site against trypsin. one of the inhibitors from phaseolus coccineus, pci 2, contains an arginine residue there. all seven phaseolus inhibitors investigated are double headed.	1976	1007623
red light-enhanced phytochrome pelletability: re-examination and further characterization.	red light-enhanced pelletability of phytochrome was observed in extracts of all 11 plants tested: avena sativa l., secale cereale l., zea mays l., cucurbita pepo l., sinapis alba l., pisum sativum l., helianthus anuus l., raphanus sativus l., glycine max (l.) merr., phaseolus vulgaris l., and lupinus albus l. this enhanced pelletability was observed in all 11 plants following in situ irradiation (in vivo binding) but only in sinapis and cucurbita after irradiation of crude extracts (in vitro bin ...	1976	16659745
lack of influence of phytochrome on membrane permeability to tritiated water.	the water permeability of tissues was investigated by measuring the efflux of (3)hho from previously loaded (in darkness) etiolated bean buds (phaseolus vulgaris l. var. red kidney), pea epicotyl segments (pisum sativum l. var. alaska), and oat coleoptile segments (avena sativa l. var. garry). red light, far red light, or darkness was applied at the time of transfer of tissue from labeled to unlabeled medium. there were no effects of light on half-time for efflux or on the maximum level of radio ...	1976	16659447
the isolation of lectins on acid-treated agarose.	the ability of several d-galactose and n-acetyl-d-galactosamine-binding lectins to bind to sepharose was investigated. lectins from soybean, wistaria floribunda, bauhinia purpurea alba, and sophora japonica could be isolated by affinity chromatography on acid-treated sepharose 6b. these lectins would not bind to untreated sepharose 2b, 4b, and 6b. the binding of b. purpurea alba and s. japonica was temperature-dependent. the s. japonica lectin would bind only at high ph. ricinus communis toxin a ...	1976	10082
selection and characterization of chinese hamster ovary cells resistant to the cytotoxicity of lectins.	chinese hamster ovary (cho) cells selected in a single step for resistance to the cytotoxicity of the lectin from red kidney beans (pha) behave as authentic somatic cell mutants. the pha-resistant (phar) phenotype is stable in the absence of selection; its frequency in a sensitive-population is increased several-fold by mutagenesis; and it behaves recessively in somatic cell hybrids. the activity of a specific glycosyl transferase which transfers n-acetylglucosamine (glcnac) to terminal alpha-ma ...	1976	1262042
immunosuppressive activity of phaseolus coccineus and phaseolus vulgaris extracts in mice.	lectin mixtures from 10 samples of phaseolus coccineus and phaseolus vulgaris beans were used as immunosuppressive agents in the sheep red blood cell (srbc) antibody response of the mouse. some bean samples contain lectins capable of inducing several times better suppression of humoral immunity than concanavalin a and phytohemagglutinin tested before in the same immune system. high dilutions of the 10 bean extracts were shown to agglutinate mouse, rabbit and sheep erythrocytes in vitro but the t ...	1976	1086791
fractionation of human t lymphocytes on wheat germ agglutinin-sepharose.	t cells from human peripheral blood was purified by fractionation on columns charged with human immunoglobulin and rabbit anti-human immuno-globulin. when assayed with 125i- or fluorescein isothiocyanate-labeled wheat-germ agglutinin (wga), a weakly binding and a strongly binding subpopulation could be distinguished. these t-cell subpopulations were fractionated on columns charged with wga, convalently bound to sepharose 6mb. the cells responding to the mitogens leukoagglutinin from phaseolus vu ...	1976	1086886
control of pyrimidine biosynthesis in human lymphocytes. inhibitory effect of guanine and guanosine on induction of enzymes for pyrimidine biosynthesis de novo in phytohemagglutinin-stimulated lymphocytes.	human peripheral lymphocytes were incubated with phaseolus vulgaris phytohemagglutinin. the induction of glutamine-utilizing carbamyl phosphate synthetase (ec 2.7.2.5) and aspartate transcarbamylase (ec 2.1.3.2) for pyrimidine biosynthesis de novo and the induction of uridine kinase were observed as described previously (ito, k., and uchino, h. (1971) j. biol. chem. 246, 4060-4065; ito, k., and uchino, h. (1973) j. biol. chem. 248, 389-392; lucas, z.j. (1967) science 156, 1237-1240). by the addi ...	1976	1254576
a method for the direct demonstration of the lectin-binding components of the human erythrocyte membrane.	1. a method which allows the characterization of lectin-binding components is described. this method should be useful in defining the nature and heterogeneity of these components in cell membranes. 2. the method, which we have used on erythrocyte "ghosts", involves the fixation of "ghost" components after sodium dodecyl sulphate/polyacrylamide-gel electrophoresis and incubation with purified 125i-labelled lectins. 3. each of the four lectins used shows an individual pattern of reactivity towards ...	1976	1275889
purification and base composition of a chloroplastic tranphe from phaseolus vulgaris.		1976	770194
the interaction of nonmitogenic and mitogenic lectins with t lymphocytes: association of cellular receptor sites.	the relationship between the surface receptors on neuraminidase-treated human blood lymphocytes for the mitogenic lectins phaseolus vulgaris leukoagglutinin (la), concanavalin a (con a) and soy bean agglutinin (sba) and the non-mitogenic lectin helix pomatia a hemagglutinin (hp) was investigated. two different techniques, co-capping with different fluorochrome-labeled lectins and cell binding-inhibition experiments with 125i-labeled lectins, were used. the results demonstrated that the nonmitoge ...	1976	772792
immunoadsorbent affinity purification of the two enzyme forms of alpha-mannosidase from phaseolus vulgaris.		1976	826414
aminoacylation of phaseolus vulgaris cytoplasmic, chloroplastic and mitochondrial trnaspro and trnaslys by homologous and heterologous enzymes.	the cytoplasmic prolyl-trna synthetase can be separated by hydroxyapatite chromatography, from the enzyme present in the chloroplasts and in the mitochondria (organellar enzyme). the cytoplasmic lysyl-trna synthetase can also be separated from the organellar enzyme. there are two trnaspro in the cytoplasm; they can be charged by the cytoplasmic enzyme, but not by the organellar enzyme or the escherichia coli enzyme. chloroplasts contain, in addition to the two cytoplasmic trnaspro, one chloropla ...	1976	953001
the binding of phytohemagglutinin m to rat spleen lymphocytes. quantitative studies.	phytohemagglutinin m (pham) has been purified from the commercial mixture of proteins produced by phaseolus vulgaris, using a sepharose-thyroglobulin column. the protein gave one band on gel electrophoresis and two bands on sds-gel electrophoresis (mol. wt. 33 700 and 32 100, respectively). molecular weight determination by ultracentrifugation gave a value of 61 200 +/- 700. the protein had a minimum sugar content of 16%. binding studies of pham to purified rat spleen lymphocytes have been perfo ...	1976	953851
immunochemical studies of infectious mononucleosis. v. isolation and characterization of a glycoprotein from goat erythrocyte membranes.	a glycoprotein was isolated from goat erythrocyte membranes by extraction with hot 75% ethanol. the glycoprotein was purified by ethanol precipitation, phosphocellulose chromatography gel filtration, ethyl:ether and chloroform:methanol extraction. in aqueous phosphate-buffered saline, ph 7, the glycoprotein was in an aggregated state with a sedimentation coefficient (s(obs)) to 1.5. electrophoresis of the glycoprotein on polyacrylamide gels containing phosphate-buffered 0.1% sds gave a single ba ...	1976	956650
[nutritional study of field bean protein isolate and of spun field bean protein-casein fibers].	the amino acid composition of the different field bean protein isolates shows a good correspondence. in relation to casein the content of the whole essential amino acids is low. in the first line the low content of methionine and cystine limits the biological value of this proteins. the content of lysine is relatively high. the enzymatic in vitro-hydrolysis results in a corresponding availability of the amino acids between the field bean proteins and casein. the digestibility is very good, the b ...	1976	958366
nutritional effects of field-bean (vicia faba l.) protease inhibitors and field beans fed to rats [proceedings].		1976	972900
comparative studies on some leguminous protein sources and soybean proteins.	the nutrient contents as well as the amino acid pattern of broad bean, kidney bean and soybean were determined. the levels of most of the amino acids were also estimated. heat treatment of the leguminous seeds caused a noticeable reduction in the levels of methionine, cystine, lysine and tryptophan. animal experiments showed high figures for digestibility, biological value and net protein utilization for the soybean diet whereas the broad bean and kidney beans gave lower values of these measurem ...	1976	987657
hybridization of bean chloroplast transfer rnas to chloroplast dna.	bean (phaseolus vulgaris) chloroplast trnasleu and trnasphe hybridize to chloroplast dna, whereas the corresponding cytoplasmic trna species do not, suggesting that chloroplast transfer rnas are coded for by chloroplast dna. the hybridization of the three chloroplast trnasleu or of the two trnasphe isoacceptors is not additive, and the isoacceptors compete with each other in the hybridization to chloroplast dna, suggesting that these isoacceptors are coded for by the same gene(s) and differ only ...	1976	999912
[evaluation of the quality of proteins of various vegetable grains used in various biological methods].	the protein value of different legume foods was studied in weanling rats by determining net protein utilization (npu), protein efficiency ratio (per), biological value (bv), nitrogen balance (nb) and digestibility of the protein. npu was determined by carcass analysis and by nitrogen determination in one of the hind legs of the experimental animals. all samples were evaluated at a 10% protein level in the ration, with and without a methionine suplement. the effect of protein concentration in the ...	1976	1008644
a preparation of field bean (vicia faba l.) cotyledons as a substitute for dried skim-milk in calf feeding [proceedings].		1976	1028050
surface alterations in calf lymphocytes oxidized by sodium periodate.	in order to investigate alterations in surface structure in transformed lymphocytes, calf submandibular lymph node cell suspensions were oxidized with naio4. oxidezed lymphocytes were morphologically transformed and had higher rates of dna synthesis by 2 days after treatment. these results were prevented by reduction of the cell suspension with nabh4, or by neuraminidase treatment of cells prior to oxidation. the amount of 125i-labeled agaricus bisporus lectin bound to cells immediately after ox ...	1976	178647
a method for eliminating the contribution of agrobacterium tumefaciens to studies of nucleic acid biosynthesis in crown gall tumors.	a procedure is described that permits the use of radiotracer methods for examining crown gall tumors in the presence of the initiating agent agrobacterium tumefaciens. on the basis of sensitivity testing using the disc diffusion technique and the minimum inhibitory concentration procedure and incorporation of radioactive phosphorus into nucleic acids, it was determined that a. tumefaciens was most sensitive to tetracycline, oxytetracycline, and methacycline. it was further demonstrated that nucl ...	1976	16659494
role of galactolipids in spinach chloroplast lamellar membranes: ii. effects of galactolipid depletion on phosphorylation and electron flow.	a galactolipid lipase from primary bean (phaseolus vulgaris) leaves has been used to partially deplete spinach chloroplast inner membranes of their galactolipids. chloroplasts treated with the lipase in the absence of bovine serum albumin lost 91% of their monogalactosyl diglyceride, 83% of their digalactosyl diglyceride, all of their phosphatidyl choline, but none of their sulfolipid. electron microscopy of this sections revealed that the treated chloroplasts were greatly enlarged and lacked me ...	1976	16659559
effect of protein additives on acetylene reduction (nitrogen fixation) by rhizobium in the presence and absence of soybean cells.	the effect of protein additives on acetylene reduction (n(2) fixation) by rhizobium associated with soybean cells (glycine max [l.] merr.) in vitro was studied. acetylene reduction was promoted on the basal medium supplemented with 1.4 mg of n/ml supplied as aqueous extracts of hexane-extracted soybean, red kidney beans (phaseolus vulgaris l.), or peas (pisum sativum l.). commercial samples of alpha-casein, or bovine serum albumin also promoted acetylene reduction at a concentration of 1.4 mg of ...	1976	16659592
mechanism of plant growth stimulation by naphthenic acid: ii. enzymes of co(2) fixation, co(2) compensation point, bean embryo respiration.	potassium naphthenate, 20 mm, was applied to the foliage of 14-day-old plants of bush bean, phaseolus vulgaris l, cv top crop, maize, zea mays l, cv golden bantam, spring wheat, triticum vulgare vill., cv neepawa, and a 2 mm solution to 21-day-old plants of sugar beet, beta vulgaris l, cv cs-43. seven days after application, the activities of ribulose diphosphate carboxylase and phosphoenolpyruvic carboxylase in leaves of naphthenate-treated bean and maize were greater than in the leaves of untr ...	1976	16659626
indoleacetic acid levels in phaseolus, zea, and pincus during seed germination.	the content of indoleacetic acid (iaa) was determined in dry and germinating seeds of french bean (phaseolus vulgaris), sugar maize (zea mays), and scots pine (pinus silvestris). iaa was found in both the dry and the soaked seeds of the three species examined. the amount of iaa per gram fresh weight was extremely different in the three species whereas the variation between different harvests of the same species was small. maize contained the highest level of iaa and bean the smallest. the time o ...	1977	16660084
measurement of ozone injury by determination of leaf chlorophyll concentration.	a simple, rapid procedure is described for evaluating ozone injury to leaves of phaseolus vulgaris l. cv. pinto. leaf chlorophyll is extracted with ethanol and analyzed spectrophotometrically; the concentration is expressed on the basis of leaf dry weight.the per cent chlorophyll reduction of ozone-injured leaves was highly correlated with the per cent visible necrosis and chlorosis (r = 0.96). the variability in injury estimates with chlorophyll analysis was slightly less than with visual evalu ...	1977	16660147
involvement of phaseolotoxin in halo blight of beans: transport and conversion to functional toxin.	phaseolotoxin ([n(delta)-phosphosulfamyl]ornithylalanylhomoarginine) is produced by pseudomonas phaseolicola (burkh.) dows. in liquid culture. when phaseolotoxin was applied to leaves of bean (phaseolus vulgaris l.) at 0.1 to 1 nmoles/g fresh weight of leaf by a prick-assay procedure, the characteristic "halo" symptom of bean halo blight disease developed after 24 to 48 hours. at higher concentrations (10-100 nmoles/g fresh weight) the systemic symptoms, which are commonly a feature of diseased ...	1977	16660172
bacteria--plant cell surface interactions: active immobilization of saprophytic bacteria in plant leaves.	fibrillar structures, originating from the plant cell wall in the intercellular spaces of leaves of ;red kidney' bean, phaseolus vulgaris l., engulfed a saprophytic bacterium, pseudomonas putida, after its initial attachment to the host walls. phytopathogenic bacteria, pseudomonas phaseolicola and pseudomonas tomato, did not adhere to the plant cell wall nor were they encapsulated. bean lectins may be involved in the attachment and encapsulation processes.	1977	17790770
nature of the lectin-induced activation of plasma membrane mg2+atpase.	the mg2+atpase activity of liver plasma membranes decreases markedly with increasing temperature above 30 degrees. this negative temperature dependency is counteracted by the binding of wheat germ agglutinin, concanavalin a, or ricinus communis agglutinin (at concentrations greater than or equal 0.5 mg/ml) to membranes prior to assay of the enzyme. with one of these lectins bound, the enzyme has a single energy of activation between 20 degrees and 45 degrees. the binding of dimeric succinyl conc ...	1977	18464
specific agglutination of escherichia coli o128b12 by the mannose-binding proteins of pseudomonas aeruginosa.	the mannosephilic haemagglutinins of pseudomonas aeruginosa were found to agglutinate cells of escherichia coli o128b12, to be adsorbed onto them and to attach peroxidase to them. these reactions were specifically inhibited by d-mannose. no agglutination by this pseudomonas haemagglutinin was obtained when several other enteropathogenic types of escherichia coli and some other gram-negative bacteria were examined. concanavalin a, which also reacted with escherichia coli o128b12 cells, interacted ...	1977	25370
influence of polyethylene glycol and related compounds on the nutritional availability of methionine in a high-tannin sorghum and in field beans.		1977	414230