Publications

[intramolecular mobility of human major histocompatibility complex protein. a spin-label study].	membranes of human splenocytes were hydrolyzed by papain and extracellular portions of class i and class ii hla antigen molecules were isolated by monoclonal antibodies fixed on sepharose 4b. the isolated proteins were spin-labeled by tempo-dichlorotriazine and the values of rotational correlation times (tau) of labeled proteins were found using dependencies of esr spectra parameters vs viscosity at constant temperature. the tau-values were equal to 8 ns for class i molecules and 14 ns for class ...	1987	2824988
evidence that mannosyl residues are involved in human immunodeficiency virus type 1 (hiv-1) pathogenesis.	the human immunodeficiency virus (hiv), the causative agent of aids, is thought to bind to t4+ (cd4+) target cells through the heavily glycosylated gp120 envelope glycoprotein. plant lectins bind glycoproteins through noncovalent interaction with specific hexose residues; therefore, lectins were evaluated for their ability to inactivate hiv in vitro. the mannose-specific lectins concanavalin-a and succinyl concanavalin-a completely inactivated hiv while lentil lectin, wheat germ agglutinin, and ...	1987	2829950
purification of envelope glycoproteins of human t cell lymphotropic virus type i (htlv-i) by affinity chromatography.	the external envelope glycoprotein (gp46) and transmembrane glycoprotein (gp21) of human t-cell lymphotropic virus type i (htlv-i) were isolated from lysates of htlv-i-infected hut-102 cells by affinity chromatography. fifty ml aliquots of packed hut-102 cells were extracted with 1% triton x-100, and lysates were treated sequentially with an affinity column containing igg from an htlv-i+ human subject followed by chromatography of the bound fraction over a lentil lectin column. the identity of t ...	1987	2832431
interaction with lectin of kappa opioid binding sites solubilized from human placenta.	kappa opioid binding sites from human placenta, prelabeled with 3h-etorphine and solubilized, were retained on wheat germ agglutinin (wga) agarose and specifically eluted with n-acetylglucosamine. no significant retention was found with other immobilized lectins, including concanavalin a (con a), soybean seed lectin (sba), pisum sativum lectin (psa), lens culinaris medik. lectin (lca), and lathyrus tingitanus lectin(lta). about 23% of applied kappa sites were specifically eluted from wga agarose ...	1987	3027488
potentiating and suppressive ige-binding factors are expressed by a single cloned gene.	we have investigated expression of an ige-binding factor (ige-bf) cdna in both cos-7 monkey kidney cells and chinese hamster ovary cells. transient expression of the ige-bf clone in either cell type yielded ige-bf, which potentiated an in vitro ige response and had an affinity for lentil lectin. in contrast, when the transient expression experiments were carried out in the presence of tunicamycin, the factors no longer bound to lentil lectin. moreover, ige-bf expressed under these conditions sup ...	1987	3027707
heterogeneity of human natural killer cells with respect to lectin-binding ability.	the binding ability of peanut agglutinin (pna), lentil agglutinin (len), soybean agglutinin (sba), wheat germ agglutinin (wga), and asparagus pea agglutinin (asp) to human natural killer (nk) cells with the use of the double-marker immunofluorescence technique was studied. for identification of nk cells, vep 13 (dc 16) monoclonal antibody was used. the receptor for pna lectin was shown exclusively after neuraminidase treatment of cells, and vep 13 antigen was neuraminidase resistant. the majorit ...	1987	3295353
bulk purification of a naturally occurring soluble form of rt1-a class i major histocompatibility complex antigens from da rat liver, and studies of specific immunosuppression.	we describe the bulk purification of a water-soluble form of rt1-a class i mhc antigens from aqueous extracts of da liver. using a combination of monoclonal antibody affinity, lentil lectin affinity, and gel permeation chromatography, we were able to obtain large quantities of pure water-soluble rt1-a antigens. typically, from 40 da livers, 0.5 mg of pure antigen with antigen activity equivalent to 7 x 10(9) nucleated da spleen cells was obtained. the water-soluble rt1-a molecule had a discrete ...	1987	3299912
boar sperm surface glycoproteins: isolation, localization, and temporal expression during spermatogenesis.	boar sperm glycoprotein fractions were isolated by lens culinaris hemagglutinin affinity chromatography of detergent-solubilized ejaculated spermatozoa, followed by preparative sodium dodecyl sulfate (sds) polyacrylamide gel electrophoresis. in order to develop methods for further investigations of the sperm proteins, we proceeded with two of the isolated glycoproteins. antibodies were raised in female rabbits against each of the two sperm glycoproteins. by a combination of immunosorbent chromat ...	1987	3333754
purification, composition, and structure of macrophage adhesion molecule.	macrophage adhesion molecule (mam) is a surface heterodimer consisting of the trypsin- and plasmin-sensitive glycopeptide gp160 (mam-alpha) and the glycopeptide gp93 (mam-beta). mam, which is the guinea pig analogue of mo1 and mac-1, was purified from detergent lysates of peritoneal neutrophils by lentil lectin chromatography and m2-antibody chromatography. the pure heterodimer molecule was dissociated by acidic conditions (ph 3.5), and mam-alpha and mam-beta were separated by m7-antibody chroma ...	1988	3349044
a quantitative method for gdp-l-fuc:n-acetyl-beta-d-glucosaminide alpha 1----6fucosyltransferase activity with lectin affinity chromatography.	a quantitative method for the activity of gdp-l-fuc:n-acetyl-beta-d-glucosaminide alpha 1----6fucosyltransferase has been developed using a well-characterized substrate to which other fucosyltransferases fail to transfer and lentil lectin-sepharose, which will bind this substrate only after fucosylation of the asparagine-linked n-acetylglucosamine. the enzyme was extracted from human skin fibroblasts and incubated with gdp-[14c]fucose and a specific substrate, asialo-agalactotransferrin glycopep ...	1988	3364733
binding site of the rat liver specific monoclonal antibody.	a rat liver-specific antigen (rlsa) lost its binding ability to the corresponding monoclonal antibody after treatment with n-glycanase or sialidase, which suggested that the specific binding site might be in a portion of the sugar chain containing sialic acid. the specific antigen reacted with wheat germ agglutinin, lentil lectin, erythroagglutinating phytohemagglutinin and ricinus communis agglutinin, but not with concanavalin a or peanut agglutinin. these results suggest that the specific anti ...	1988	3377770
isoelectric properties, lectin binding characteristics and biological activities of neuraminidase-treated rat lh components.	the present study was performed to evaluate the different carbohydrate structure of rat lh isoelectric components related to their intrinsic biological activities. terminal sialic acid residues were essential to the formation of multiple lh components observed in the isoelectric focussing profile, which was proved by their interaction with ricinus communis agglutinin-120 following neuraminidase treatment, and the conversion of component f (pi, 10.0) to less alkaline components after incubation w ...	1988	3381629
analysis of cell surface glycoprotein changes related to hematopoietic differentiation.	a high-resolution technique has been used to study differentiation-related and leukemia-associated glycoproteins. cells are labeled with the membrane-impermeable probe sulfo-n-hydroxysuccinimidyl-biotin. nonionic detergent extracts are subjected to affinity chromatography on a number of immobilized lectins and after polyacrylamide gel electrophoresis in sodium dodecyl sulfate (sds-page) and western transfer, the biotin-labeled glycoproteins are visualized by using avidin-horseradish peroxidase a ...	1988	3392108
the identification of membrane glycoconjugates in leishmania species.	the membrane glycoconjugates of 8 different species of leishmania were compared by lectin blotting. five different lectins with various sugar specificities were examined: concanavalin a, lens culinaris, ricinus communis, soybean agglutinin, and peanut agglutinin. concanavalin a and lens culinaris reacted with every leishmania tested. the patterns observed for these 2 lectins, as well as the various species of parasites, were different. however, a common 41,000-52,000 and a 160,000-185,000 mr com ...	1988	3397816
complex-type glycoproteins synthesized in the subcommissural organ of mammals. light- and electron-microscopic investigations by use of lectins.	the secretory activity in the subcommissural organ (sco) of the sheep and cow was examined by means of lectin histochemistry and cytochemistry. among the various lectins tested. concanavalin a (con a) revealed glycoproteins rich in mannosyl residues in the rough endoplasmic reticulum of ependymal and hypendymal cells. one of these con a-positive glycoproteins may represent the precursor of the specific secretory component elaborated in the sco, giving rise to reissner's fiber. lens culinaris agg ...	1988	3409290
lectin-induced adhesion of human platelets to glass. comparison with the lectin-induced aggregation.	effects of phytohaemagglutinin (pha), concanavalin a (con a) and lens culinaris (lens) lectins on the adhesion of human platelets to glass surface were studied. all the three lectins tested enhanced platelet adhesion to glass. concentration-effect curves showed that maximum effect was reached at about 25-30 micrograms/ml. in the presence of autologous plasma both spontaneous and lectin-induced adhesion to glass were diminished. the inhibitory effect of kcn, nan3, cytochalasin b and partly of col ...	1988	3417184
aflatoxins and fungal flora in lentil (lens esculenta l.).	fungal flora of 20 samples of lentil seeds collected from assiut governorate, egypt, were studied. seventeen genera and 13 species were isolated on glucose- (15 genera and 27 species), cellulose- (15 genera and 25 species)- czapek's agar media at 28 degrees c. the most common species were as follows: on glucose-czapek's agar, aspergillus fumigatus, a. niger, a. flavus, a. terreus, penicillium notatum and rhizopus oryzae and on cellulose agar, a. fumigatus, a. niger, a. flavus and p. notatum. thi ...	1988	3419482
importance of glycosylation for hepatic clearance of renal renin.	three differently glycosylated forms of renin (renin a, b-1, and b-2) were highly purified from rat kidneys by pepstatin-aminohexyl-sepharose affinity chromatography and by serial lectin affinity chromatography on concanavalin a (con a) and lentil lectin-sepharose, and the role of glycosylation of renin was investigated. renin a and renin b-1 were loosely and tightly bound to con a, respectively, but did not bind to lentil lectin. renin b-2 bound to both con a and lentil lectin. these three form ...	1988	3056032
differently glycosylated multiple forms of renin in the blood circulation of rats.	serial lectin chromatography of rat plasma on concanavalin a and lentil lectin columns separated plasma renin into differently glycosylated multiple forms. to study the role of glycosylation in the clearance of circulating renin, three different forms of glycosylated renin (renin a, b-1 and b-2) were highly purified from rat kidneys. renin a and b-1 were loosely and tightly, respectively, bound to concanavalin a, but did not bind to lentil lectin. renin b-2 was bound to both concanavalin a and l ...	1988	3071581
administration of a 600 kd molecular fraction from pancreatic islets suppresses immune mediated diabetes in mice.	treatment of male c57bl6/j mice with low doses of streptozotocin causes the development of diabetes. infiltration of pancreatic islets by immune cells and the concomitant loss of beta islet cells can be prevented by immunosuppression. we now report that i.v. administration of islet homogenate 13 d prior to low dose streptozotocin treatment largely protects from development diabetes. homogenates from liver, kidney or exocrine pancreas were without effect. the suppressive activity was isolated par ...	1988	3075128
surface movements during the spreading of blood platelets.	when human blood platelets spread on a substratum they increase their surface area as much as 4-fold. we investigated the mechanism of spreading by light microscopy and by scanning and transmission electron microscopy. contact of a platelet with a glass surface induces formation of thin extensions which spread out over the substratum. these extensions resemble the actin-containing microspikes and lammelipodia of tissue cells in culture and appear to be drawn from the peripheral cortical layer as ...	1988	3169030
altered expression of platelet surface glycoproteins during storage.	human blood platelets were stored in autologous plasma at 4 degrees c or 22 degrees c and their surface changes were probed with three lectins--wheat germ agglutinin, lentil lectin and concanavalin a. platelets stored at either temperature for different times showed increased sensitivity to lectins. lectins which were nonagglutinating to fresh platelets readily agglutinated stored platelets. the platelets stored for 24 h or longer lost their ability to respond to thrombin but demonstrated enhanc ...	1988	3179230
biochemical properties of a 24 kilodalton membrane glycoprotein antigen complex from schistosoma mansoni.	antibodies were affinity purified from crude antiserum by elution from the 24 kda region of preparative one-dimensional western blots containing immobilized adult schistosoma mansoni inner bilayer membrane proteins. they were shown to be specific for a single acidic polypeptide complex, smgp24, following immunoblotting from two-dimensional polyacrylamide gels. these antibodies were then used to detect the presence of the smgp24 complex in fractions prepared from lectin affinity chromatography, p ...	1988	3185620
disulfide-linked receptors for mitogenic lectins on piglet lymphocytes.	the interaction between leucoagglutinating phytohemagglutinin (l-pha), concanavalin a (con a), soybean agglutinin (sba) and lentil lectin (lch) with disulfide-linked cell surface receptors on lymphocytes from mesenteric lymph nodes of 3-day piglets (pmln) was investigated. surface radioiodinated pmln lymphocytes were lysed with buffer containing nonidet-p40. the lysates were adsorbed on lectin-agarose derivatives (or bovine serum albumin-agarose). eluates from the lectin-agarose derivatives were ...	1988	3191945
pneumovirus-like characteristics of the mrna and proteins of turkey rhinotracheitis virus.	electronmicroscopy has indicated that turkey rhinotracheitis virus (trtv), the causative agent of an acute respiratory disease in turkeys, is a member of the paramyxoviridae family. to determine if trtv belongs to one of the three defined genera of this family (paramyxovirus, morbillivirus and pneumovirus) we have analysed the rna and proteins induced during replication of trtv in vero cells. following replication in the presence of actinomycin d 10 polyadenylated rna bands, ranging in mr from 0 ...	1988	3195218
an ultrastructural study of the morphology and lectin-binding properties of human mast cell granules.	the morphological characteristics and lectin-binding properties of mast cell granules from four human neurofibromata are described. ultrastructural examination of the granules revealed that some contained dense cores, others had membranous configurations and some forms were intermediate between the two. a round electron-lucent area was present in some granules. after treatment with biotinylated lectins (10 micrograms ml-1) followed by an avidin-peroxidase revealing system (5 micrograms ml-1 in 0 ...	1988	3198420
[localization of glycoconjugates in tissues of mammals revealed by means of labeled lectins].	histochemical peculiarities on binding of castor-oil plant, soybean and lentil lectins with tissues of the mucous membrane in the stomach, small and large intestine have been studied in the human being, rat, mouse, as well as the lectins mentioned and the maize agglutinin with the nervous tissue of the rat cerebral tissue. the reactions are carried out with nonfixed cryostat and deparaffinized histological slices. lectins labelled with horseradish peroxidase are used. certain specific peculiarit ...	1988	3207398
comparative chromatographic study of modifications of brush-border membrane vesicles induced by an essential fatty acid-deficient diet.	the surface properties of small intestine brush-border membranes (bbms) were examined by frontal affinity chromatography using three types of unsolubilized ligands: phlorizin polymer, immobilized lectins and linolenic acid bound to agarose gel. bbm vesicles were purified from piglets fed a corn oil diet (control diet) or a hydrogenated coconut oil diet. the second diet was representative of a deficient supply of essential polyunsaturated fatty acid (epufa). it induced a marked decrease in 18:2n- ...	1988	3220917
heterogeneity in copper and glycan content of ceruloplasmin in human serum differs in health and disease.	crossed immunoelectrophoresis of human serum revealed two heterogeneity types of ceruloplasmin with different electrophoretic migration. the two types both consisted of peptides with mr 150,000, 100,000 and 45,000, which were interpreted as native ceruloplasmin and two hydrolytic fragments. the two types were different in copper content, and one type could reversibly be changed into the other. the glycan microheterogeneity of ceruloplasmin was analyzed by crossed affinoimmunoelectrophoresis with ...	1988	3234365
the rat t-cell differentiation marker rt6.1 is more polymorphic than its alloantigenic counterpart rt6.2.	utilizing allotype-specific antibodies to immunoprecipitate rt6.2 from da.6b rat lymphocyte lysates, we have shown this antigen recently to be composed of two related, non-glycosylated polypeptides with apparent molecular weights (mw) of 24,000 and 26,000 (reducing conditions), which evidently are anchored in the cell membrane by covalent linkage to phosphatidylinositol. the present report shows that rt6.1 allotype-specific antibodies precipitate a more complex pattern of bands from lew.6a rat l ...	1988	3263942
regulatory effects of human ige-binding factors in the ige synthesis by human and rat lymphocytes.	we have previously established human t cell hybridomas which produce ige-binding factors. incubation of one of the t cell hybridomas, 166a2, with human ige dimer in the presence of 1 microgram/ml bradykinin resulted in the formation of ige-binding factors having affinity for lentil lectin. the factors selectively enhanced both ige-forming cell responses of rat mesenteric lymph node (mln) cells and spontaneous ige synthesis by human peripheral blood b cells of atopic patients, without affecting t ...	1988	3264529
application of glycemic index to mixed meals.	plasma glucose and insulin responses to six different meals were determined and compared with values predicted by published glycemic indices of the component foods. the test meals were of different ethnic origins: indian (lentil curry with rice), italian (spaghetti bolognaise), chinese (stir-fried vegetables and chicken with rice), greek (lentil stew), western (sirloin chop and vegetables); and lebanese (sandwich with unleavened bread and hummos). eight healthy volunteers were given 50 g carbohy ...	1988	3276136
lectin-binding histochemistry of intracellular and extracellular glycoconjugates of the reserve cell zone of growth plate cartilage.	the distribution of intracellular and extracellular lectin-binding glycoconjugates of the reserve cell zone of growth plate cartilage was studied in the distal radial growth plate of 4-week-old yucatan swine using a postembedment method on epon-embedded sections. direct comparisons were made to articular, tracheal, and auricular cartilages not involved in endochondral ossification. all patterns of lectin binding that in the growth plate were restricted to the reserve cell zone were also patterns ...	1988	3278077
investigations on the binding of erythrogenic toxin a of streptococcus pyogenes on human peripheral blood lymphocytes. ii. identification of toxin-binding lymphocytes and characterization of the receptor.	gold complexes of erythrogenic toxin a (eta) of streptococcus pyogenes (strain ny-5) were bound to human lymphocytes that could be characterized as t cells by rosetting or labelling with okt 3 antibody and anti-mouse-igg-gold. using okt 4 and okt 8 antibodies, respectively, it was demonstrated that binding of eta was confined to the t helper/inducer cell subpopulation. treatment of lymphocytes with neuraminidase enhanced the binding of eta-gold, whereas trypsin treatment resulted in its reductio ...	1988	3287805
nephritogenicity and immunocytochemical localization of the 70-kilodalton glycoprotein subunit (gp70) of heymann antigen.	heymann nephritis (hn) is an experimentally induced glomerulonephropathy of the rat characterized by subepithelial immune deposits and proteinuria. immunization with a complex multimeric glycoprotein, gp600, comprising four subunits gp330, gp140, gp110, and gp70 has been shown to induce the complete form of the disease including proteinuria. examination of three different batches of heterologous anti-gp600 antisera by immunoblot technique showed that the reactivity toward gp70 was dominant and c ...	1988	3289803
a quantitative analysis of lectin binding to adult rat hepatocyte cell surfaces.	a quantitative evaluation of lectin binding to adult rat hepatocyte cell surfaces was done using cells isolated by two different collagenase perfusion methodologies and cultured as monolayers with two different tissue culture media formulations (protocol i vs. protocol ii). the presence of alpha-d-mannosyl and alpha-d-glucosyl groups was detected by the binding of concanavalin a (con a), lens culinaris agglutinin (lca), and pisum sativum agglutinin (psa) to freshly isolated cells. furthermore, b ...	1988	2836355
the use of lectin affinity columns for selection of precursor or fully glycosylated forms of glycoprotein gd1 of herpes simplex virus type 1.	several lectins were examined for their ability to bind to the glycoprotein gd1 polypeptide from vero cells infected by herpes simplex virus type 1 (hvs-1), strain kos. at least four distinct forms of gd1 (1, 2, 3 and 4), ranging in size from 59k to 52k, were resolved by sds-10% polyacrylamide gel electrophoresis. wheat germ agglutinin (wga) did not bind to any of these forms, suggesting that if any sialic residues are present in the carbohydrate moieties of gd1, they are not available for bindi ...	1988	2840449
hepatocyte cell surface polarity as demonstrated by lectin binding.	we performed an investigation at the ultrastructural level of the differential distribution of lectin-binding sites among sinusoidal, lateral, and bile canalicular domains of adult rat hepatocytes. lectin binding to hepatocyte glycocalices was studied in situ or after cellular dissociation by enzymatic (collagenase), chemical (edta), and mechanical methods, as well as during cell culture. using thirteen biotinylated lectins and an avidin-biotin-peroxidase complex (abc), we have identified lectin ...	1988	2848070
characteristics of the minor group receptor of human rhinoviruses.	the receptor for the minor group of human rhinoviruses was solubilized from hela cell membranes with various detergents. virus binding activity was determined in a filter binding assay using 35s-labeled human rhinovirus 2 (hrv2) as a probe. the receptor protein was enriched on lens culinaris lectin columns and the active fractions were further purified by gel permeation and anion exchange chromatography. the receptor has an apparent molecular weight of 450 kda in the presence of detergent. the b ...	1988	2831654
squid glycoproteins with structural similarities to thy-1 and ly-6 antigens.	in an attempt to identify invertebrate homologs of thy-1 antigen, the optic and central nervous tissue of squid was solubilized in deoxycholate and fractionated by lentil lectin affinity chromatography and gel filtration to yield small abundant glycoproteins. material with biochemical similarities to thy-1 was found and shown to consist of two glycoproteins that were ultimately purified using monoclonal antibody affinity columns. both glycoproteins were sequenced to yield sequences of 84 residue ...	1988	2894353
suppressive substance produced by t cells from mice chronically infected with trypanosoma cruzi. ii. partial biochemical characterization.	culture supernatants of splenic t cells from susceptible cba mice chronically infected with trypanosoma cruzi contain a suppressive substance which can inhibit the induction of delayed-type hypersensitivity (dth) to a wide range of antigens. the suppressive substance is distinct from t. cruzi antigen inasmuch as the supernatant depleted of any residual t. cruzi antigen by an affinity column still retains the suppressive activity, whereas addition of t. cruzi antigens to control supernatant did n ...	1988	3123553
[biosynthesis and main biological properties of human gamma interferon].	conditions for and regularities of gamma-interferon production were elucidated. high interferon inducing activity of the known inductors such as concanavalin a, phytohemagglutinin and lentil lectin was asserted. it was shown that aqueous and alcoholic extracts of legumes seeds were also able to induce interferon synthesis in cultures of human peripheral blood leukocytes. by physicochemical properties such as stability to acids and liability to heating and by antigenic properties, interferon prod ...	1988	3132115
a burst-promoting activity derived from the human bone marrow stromal cell line km-102 is identical to the granulocyte-macrophage colony-stimulating factor.	recently, several human bone marrow stromal cell lines have established and produced hematopoietic growth factors. one of these factors, a burst-promoting activity (bpa), was purified from 6 liters of serum-free conditioned medium cultured from stromal cell line km-102, which was stimulated by phorbol myristate acetate (pma) and calcium ionophore a23187. this stimulation induced 60 times more production of bpa than the unstimulated control culture. bpa was purified 4000-fold by sequential fracti ...	1988	3134250
quantitative studies of changes in cortical granule number and distribution in the mouse oocyte during meiotic maturation.	cortical granules (cgs) undergo a substantial change in distribution in the mouse oocyte cortex during meiotic maturation. in order to determine the mechanism of their change in distribution near the time of ovulation, cg density, total number per oocyte, and domain areas were quantitated. cgs were visualized microscopically by lens culinaris agglutinin-biotin and texas red-strepavidin fluorescence as well as by electron microscopy. immature germinal vesicle stage (gv) oocytes from adult mice ha ...	1988	3141231
purification of a murine leukemia inhibitory factor from krebs ascites cells.	a factor capable of inducing terminal differentiation in the murine myeloid leukemia cell line m1 has been purified to apparent homogeneity from the medium conditioned by krebs ii ascites tumor cells. the factor, termed leukemia inhibitory factor (lif) is a single chain glycoprotein of apparent mr 58,000 which induces differentiation and inhibits proliferation of the m1 cell line but not the wehi-3b d+ murine myeloid leukemic cell line and has no detectable proliferative activity on normal myelo ...	1988	3142300
surface carbohydrate changes on onchocerca lienalis larvae as they develop from microfilariae to the infective third-stage in simulium ornatum.	use was made of seven fitc labelled lectins as tools to investigate the surface of onchocerca lienalis larvae as they develop through to the infective third-stage in a natural vector, simulium ornatum. the lectins were derived from canavalia ensiformis (con a), lens culinaris (lentil), triticum vulgaris (wheat germ), arachis hypogaea (peanut), helix pomatia, phaseolus vulgaris (kidney bean) and tetragonolobus purpureus (asparagus pea). between 70 and 100 living parasites were examined for each d ...	1988	3142948
structural and functional analysis of cr2/ebv receptor by means of monoclonal antibodies and limited tryptic digestion.	the receptor for the c3d fragment of the third component of complement, cr2, has recently been shown also to act as the receptor for the epstein-barr virus (ebv) and to be involved in the control of b-cell proliferation. in order to define functionally important domains on this molecule, we produced monoclonal antibodies to several distinct epitopes. cr2 was purified from a np-40 lysate of human tonsils by a new method involving sequential chromatography on lentil lectin sepharose 4b and deae-se ...	1988	2448232
expression of thy-1 and effect of phosphatidylinositol-specific phospholipase c on primate and murine cell lines.	balc/c mice were immunized with isolated human brain thy-1. the antisera at an appropriate dilution only reacted with a doublet of an apparent molecular weight (mw) around 25,000 among all the glycoproteins of brain tissue isolated by lentil lectin affinity chromatography when tested by enzyme-linked immunosorbent assay and immunoblotting. when the antisera were used to test a number of human cell lines and a marmoset t-cell line (70n2) by flow cytometry, an astrocytoma cell line (u-373), a t-ly ...	1988	2451568
leishmania donovani: identification of glycoproteins released by promastigotes during growth in vitro.	culture supernatants of metabolically labeled leishmania donovani promastigotes were shown to contain approximately 40 electrophoretically distinct released protein compounds. of these, approximately 20 were glycoproteins which contained terminal mannose residues, as judged by their specific binding to concanavalin a-agarose beads. smaller subsets of the released glycoproteins were bound by agarose-conjugated lens culinaris, ricinus communis, and peanut lectins. promastigote mannose-containing r ...	1988	2461315
glycoconjugates in normal human kidney. a histochemical study using 13 biotinylated lectins.	the avidin-biotin-peroxidase complex technique was used with 13 lectins to study the glycoconjugates of normal human renal tissue. the evaluated lectins included triticum vulgaris (wga), concanavalin ensiformis (cona), phaseolus vulgaris leukoagglutinin and erythroagglutinin (pha-l and pha-e), lens culinaris (lca), pisum sativum (psa), dolichos biflorus (dba), glycine max (sba), arachis hypogaea (pna), sophora japonica (sja), bandeiraea simplicifolia i (bsl-i), ulex europaeus i (uea-i) and ricin ...	1988	2466021
characterization of a soluble protein of coccidiodes immitis with activity as an immunodiffusion-complement fixation antigen.	a 48-kilodalton (kda) electrophoretically distinct antigen from coccidioides immitis mycelial- and spherule-endospore-phase filtrates was previously associated by immunoblotting with the immunodiffusion band that corresponds to complement-fixing activity (id-cf). to characterize this antigen and its precursor, both mycelial- and spherule-endospore-phase filtrates were fractionated by size exclusion chromatography, lectin affinity chromatography, and nondenaturing electrophoresis. by size exclusi ...	1988	3148631
purification of the beta subunit of the fibronectin receptor.	in this work we describe a method for purification of the beta subunit of the mouse fibronectin receptor (gp135). cellular glycoproteins were isolated from a detergent extract of sr-balb tumor cell membranes by two steps of affinity chromatography on lentil lectin-sepharose and wheat-germ-agglutinin--agarose. this material was subsequently bound to an affi gel 102 column and eluted by increasing salt concentration. most of the gp135 was eluted at 80 mm sodium chloride together with a few other c ...	1988	2965011
purification, characterization, and immunochemical studies of beta-n-acetyl-d-hexosaminidase from the parasitic nematode trichinella spiralis.	the exoglycosidase, beta-n-acetyl-d-hexosaminidase was purified 600-fold from the muscle-stage larvae (l1) of trichinella spiralis. on sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page), the purified enzyme-active fraction contained 4 polypeptides with apparent molecular weights of 100,000, 68,000, 58,000 and 54,000. the beta-n-acetyl-d-hexosaminidase corresponds to the mr 100,000 polypeptide as demonstrated by sds-page analysis of the enzyme-stained region isolated from a non- ...	1988	2972930
the separation of immunocyte subpopulations by use of various lectins.	the use of lectins for the enrichment of various lymphocyte subpopulations was investigated. bauhinia purpurea lectin (bpa) was found to be effective for the enrichment of b cells, and the b cells thus obtained were further fractionated with lentil lectin into subsets showing high and low responses to dextran sulfate. the ability of various lectins to selectively induce suppressor t cell activity or helper t cell activity was also examined. the suppressor t cells thus induced were enriched peanu ...	1988	3051929
phytochrome-mediated control of diamine oxidase level in the epicotyl of etiolated lentil (lens culinaris medicus) seedlings.	diamine oxidase (dao; ec 1.4.3.6) levels are strongly reduced in epicotyls of 3-day-old etiolated lentil (lens culinaris medicus) seedlings upon exposure to continuous red and blue light, as compared to etiolated controls. far-red light inhibits dao activity to a lesser extent. a less marked effect can also be obtained by short (5-10 min) daily exposures. phytochrome involvement in this light-mediated response has been demonstrated by red/far-red reversibility experiments. these findings provide ...	1988	16666444
affinity chromatography of fibrinogen on lens culinaris agglutinin immobilized on cnbr-activated sepharose: study of the active groups involved in nonspecific adsorption.	fibrinogen binds specifically to lens culinaris agglutinin coupled to cnbr-activated sepharose. however, a fraction of the retained fibrinogen remains tightly bound to the gel and is eluted only by electrophoretic desorption. the irreversible binding of fibrinogen results from the interaction of fibrinogen specifically bound to the immobilized lectin with some reactive groups still present on the sepharose matrix. therefore, the active groups present on lectin-sepharose after different treatment ...	1988	3218727
[t-cell immunodeficiency in mice receiving lectin and cyclophosphamide].	cyclophosphamide injections to mice following t cell mitogen (lectins from lens culinaris and concanavalin a) were shown to suppress (20-40-fold) thymus-dependent response to srbc. at the same time no damage-specific and polyclonal response to thymus-independent antigen and polyclonal activator of b cells--lipopolysaccharide--has been observed. injections of lectin and cyclophosphamide to mice prevented the onset of dth reaction to srbc and induction of antigen-specific dth suppressor cells. thu ...	1988	3261177
localization of cortical granule constituents before and after exocytosis in the hamster egg.	electrical activation of the hamster egg was used to study cortical granule constituents before and after exocytosis. the activated hamster eggs underwent cortical granule decondensation just prior to and at the time of exocytosis. some of the cortical granules of aged, unactivated eggs underwent similar changes. fitc- and gold-conjugated lens culinaris agglutinin (lca) bound intensely to the surfaces of activated but not unactivated eggs. this labelling was associated with the microvilli. perme ...	1988	3133448
distribution of polyamines and their related catabolic enzyme in etiolated and light-grown leguminosae seedlings.	diamine-oxidase (dao; ec 1.4.3.6) activity and di-and polyamine levels were estimated along the epicotyl and root of light-grown and etiolated lentil (lens culinaris medicus) and pea (pisum sativum l.) seedlings. the activity of dao was higher in etiolated epicotyls than in lightgrown ones. in both species there was a positive correlation between dao activity and the diamine (putrescine and cadaverine) levels along the whole epicotyl and root. polyamine (spermine and spermidine) distribution see ...	1988	24226538
cell surface biochemical and metastatic properties of lens culinaris hemagglutinin-binding variants of a murine large cell lymphoma.	using a low metastatic potential parental (p) line of the murine large cell lymphoma raw117 and a highly metastatic in vivo-selected liver-colonizing subline (h10), we examined the relationship between cell surface glycoprotein expression and metastasis. the highly metastatic h10 cells showed loss of the major rna tumor virus envelope glycoprotein gp70 and increased expression of a concanavalin a and lens culinaris hemagglutinin (lch)-binding glycoprotein of mr approximately 15,000 (gp150) by le ...	1988	3265705
autolysis of protein bodies in germinating lentil seeds.	protein bodies isolated from lentil (lens culinaris, medik) cotyledons exhibit autolytic activity which increases during seed germination. such autolytic capacity is active across a broad ph range and shows a maximum at ph 6.5. excision of the embryonic axis reduces autolytic capacity and application during incubation of the seeds without axis of both 6-benzylaminopurine and kinetin is able to replace it. on the other hand, the proteolytic activity in the protein body membrane, is located toward ...	1989	2616873
fractionation of plasmic fibrin(ogen) digests by lectin affinity chromatography.	a method for the fractionation of plasmic digests of both fibrinogen and fibrin was developed by taking advantage of the different chromatographic behaviour of fibrinogen and its fragments on immobilized concanavalin a and lens culinaris agglutinin. columns with different lectin concentration but with the same total lectin content were tested. fragment e was retained on all the concanavalin a-sepharose preparations while fragment d was mostly eluted in the unbound fraction. however, the binding ...	1989	2781526
variability for restriction fragment lengths and phylogenies in lentil.	thirty accessions of domesticated (lens culinaris ssp. culinaris) and wild (l. culinaris ssp. orientalis, l. culinaris ssp. odemensis, l. nigricans ssp. ervoides and l. nigricans ssp. nigricans) lentil were evaluated for restriction fragment length polymorphisms (rflps) using ten relative low-copy-number probes selected from partial genomic and cdna libraries of lentil. nei's average gene diversity was used as a measure of genetic variability for restriction fragment lengths within subspecies an ...	1989	24232901
differential response of adherent and unanchored melanoma cells to bromodeoxyuridine evidenced by specific lectin-binding protein changes.	the possible differential response of adherent and nonadherent cells of the same tumor type to pyrimidine analogues has been investigated. we show that bromodeoxyuridine (budr) increases interactions of attached cells with their substrate without markedly affecting the cell adhesion properties of the same cells when these are not anchored. however, evidence for an adhesion-independent response of both cell types to budr has been obtained with lectin binding assays using 125i-labelled lens culina ...	1989	2714888
further characterization of monoclonal antibody 6,f-8 reacting with lathyrus, lens and pisum lectins.	the murine monoclonal antibody (moab) 6,f-8 made against the glucose/mannose-specific lathyrus odoratus mitogen has previously been shown to react with lens culinaris and pisum sativum lectins, but not with the lectin from vicia faba [(1988) biol. chem. hoppe-seyler 369, 365-370]. the reactivity against seven other completely sequenced lathyrus lectins has now been tested after separation of the subunits by sds-polyacrylamide gel electrophoresis and electroblotting to nitrocellulose filters. two ...	1989	2468535
polarity of statocytes in lentil seedling roots grown in space (spacelab d1 mission).	a morphometric analysis of root statocytes was performed on seedlings of lentil (lens culinaris l., cv. verte du puy) in order to determine the effects of microgravity on the polarity of these cells. seedlings were grown: (1) on the ground, (2) in microgravity, (3) on a 1 g centrifuge in space, (4) first in microgravity and then placed on a 1 g centrifuge for 3 h. dry seeds were hydrated in space (except for the ground control) for 25 h in darkness at 22 degrees c in the biorack facility develop ...	1989	11541142
linkages between restriction fragment length, isozyme, and morphological markers in lentil.	a genetic linkage map of lentil comprising 333 centimorgans (cm) was constructed from 20 restriction fragment length, 8 isozyme, and 6 morphological markers segregating in a single interspecific cross (lens culinaris × l. orientalis). because the genotypes at marker loci were determined for about 66 f2 plants, linkages are only reported for estimates of recombination less than 30 cm. probes for identification of restriction fragment length polymorphisms (rflps) were isolated from a cdna and ecor ...	1989	24232618
clonal analysis of the glycosylation of immunoglobulin g secreted by murine hybridomas.	a panel of 10 hybridomas was assembled to assess the influence of various genetic and biological factors upon glycosylation of secreted monoclonal igg. after exhaustive pronase digestion of igg, glycopeptides were characterized chromatographically by apparent size, charge, and concanavalin a (con a)-sepharose and lens culinaris (lch)-agarose affinity. six glycosylation phenotypes were found to be common among all clones studied. despite this phenotypic heterogeneity in glycosylation of igg, cons ...	1989	2713370
[study of the carbohydrate components of the surface of s 1300 neuroblastoma cells with the use of cytochemical and lectin agglutination methods].	the carbohydrate determinants of the cell surface of neuroblastoma c 1300 clone n 18 have been investigated by light microscopy with a panel of 11 lectins as cytochemical reagents. the expression of n- and o-glycanes with a predominance of 3-4 antigenic n-glycosyl chains was revealed. the characteristic feature of neuroblastoma cells is an abundance of an antigen a surface determinant. a strong correlation is shown between the intensity of lectin binding and index of cell agglutination. after th ...	1989	2791953
the notch gene product is a glycoprotein expressed on the cell surface of both epidermal and neuronal precursor cells during drosophila development.	the notch locus of drosophila melanogaster is one of a small number of zygotically acting "neurogenic" genes involved in the correct segregation of neural from epidermal lineages during embryogenesis as well as in other postembryonic developmental events. we have generated antibody probes against three regions of the notch protein to study the expression of notch and begin a biochemical characterization of the protein. consistent with predictions based on dna sequence data, here we gather eviden ...	1989	2509485
evidence of altered structural and secretory glycoconjugates in the jejunal mucosa of patients with gluten sensitive enteropathy and subtotal villous atrophy.	the pattern of lectin histochemistry in formalin fixed, paraffin embedded normal jejunal and subtotal villous atrophy specimens from patients with gluten sensitive enteropathy were compared. there was no significant difference in the binding pattern of five lectins (arachis hypogaea, canavalia ensiformis, lens culinaris, phaseolus vulgaris and triticum vulgaris) between normal and abnormal specimens. there were significant changes in the binding pattern of three lectins (dolichos biflorus, ulex ...	1989	2753405
amine oxidase from lathyrus cicera and phaseolus vulgaris: purification and properties.	cu-amine oxidases (amine oxygen oxidoreductase deaminating, copper containing e.c. 1.4.3.6.) are found in all forms of life (1). they catalyze the following general reaction: r-ch2-nh2 + o2 + h2o----r-cho + nh3 + h2o2. cu-amine oxidases (cu-aos) have been extracted from different leguminosae: pisum sativum (2-3), lathyrus sativus (4), lens esculenta (5), vicia faba (6), cicer arietinum (7), glycine max (8) but not from phaseolus vulgaris. palavan and galston (9), in a study of polyamine biosynth ...	1989	2798365
human melanoma cell attachment involves an arg-gly-asp-directed adhesion receptor and the disialoganglioside gd2.	the disialogangliosides gd2 and gd3 play a major role in the ability of human melanoma cells to attach to arg-gly-asp-containing substrates such as fibronectin and vitronectin, since pretreatment of these cells with monoclonal antibodies to the oligosaccharide of gd2 and gd3 can inhibit their attachment and spreading on such adhesive proteins. this report demonstrates that human melanoma cells (m21) synthesize and express a glycoprotein receptor that shares antigenic epitopes with the vitronecti ...	1989	2470110
binding of lectins to human leukaemic cells.	the interactions of five different lectins: peanut (pna), lentil (len), wheat germ (wga), soybean (sba), asparagus pea (fbp) with leukaemic cells obtained from 31 children: 25 with acute lymphoblastic leukaemia (all) and 6 with acute myeloblastic leukaemia (aml) were examined in this study. the relationship of lectin-binding ability to cells cytomorphological, cytochemical and immunological features and its potential clinical application were investigated. it has been shown that pna and len rece ...	1989	2475405
carbohydrate composition of beta-core.	beta-core is a major component of the hcg-related molecules found in pregnancy urine. we previously have purified the beta-core molecule and have deduced portions of its carbohydrate structure based on lectin binding data. in the present study we used recently developed technology to determine the carbohydrate composition of beta-core and hcg beta (cr119). for direct compositional analysis, parallel samples were hydrolyzed in trifluoroacetic acid and analyzed for sialic acid and neutral sugars w ...	1989	2477227
cytotactin and its proteoglycan ligand mark structural and functional boundaries in somatosensory cortex of the early postnatal mouse.	the expression of the extracellular matrix molecules cytotactin, which is synthesized by glia, and cytotactin-binding (ctb) proteoglycan, which is synthesized by neurons, was examined in the developing brain of the mouse, specifically in the cortical barrel field, using highly specific polyclonal antibodies to the purified molecules. both molecules appeared early in the development of the cortex but were excluded from the centers of the developing barrels at the time of entry and arborization of ...	1989	2479585
two-dimensional lectin affinity electrophoresis of alpha-fetoprotein: characterization of erythroagglutinating phytohemagglutinin-dependent microheterogeneity forms.	by means of two-dimensional lectin affinity electrophoresis of human alpha-fetoprotein (afp) from different sources, afp bands separated with erythroagglutinating phytohemagglutinin (e-pha) were further characterized with other lectins of known oligosaccharide specificities. the results with a cord serum afp revealed that not only afp-p2 (e-pha-nonreactive) but also afp-p4 and p5 (e-pha-reactive) had affinities for concanavalin a (con a) and allomyrina dichotoma lectin (allo a), indicating that ...	1989	2482176
[electrophoretic analysis of urinary glycoproteins in diabetic nephropathy using peroxidase-lectins].	we examined the clinical usefulness determined by polyacrylamide gel electrophoresis, followed by reaction with peroxidase-coupled lectins using urinary glycoproteins for diabetic nephropathy in 20 patients with diabetes mellitus. lectins used were triticum vulgaris (wga), phaseolus vulgaris (pha-e4), dolichos biflorus (dba), and lens culinaris (lca), which have high affinity for beta 1----4n-acetyl-d-glucosamine (glcnac beta 1----4glcnac), n-acetyl-d-galactosamine (galnac), alpha-galactosamine ...	1989	2483179
characterization and localization of the sporulation glucoamylase of saccharomyces cerevisiae.	glucoamylase (sga) was purified approximately 250-fold from sporulating saccharomyces cerevisiae cells. the partially purified enzyme was active against glycogen, starch, maltotriose and maltose. it exhibited maximum catalytic activity against glycogen at ph 5.5. the enzyme appears to be glycosylated, because it bound to lentil-lectin sepharose. sga was expressed in vegetatively growing cells under the control of the gal1 promoter, and the cellular location of the enzymatic activity determined b ...	1989	2493265
inhibin and activin-like activity in fluids from male and female gonads: different molecular weight forms and bioactivity/immunoactivity ratios.	the existence of various molecular weight forms of inhibin in ovarian follicular fluid has been reported earlier, while there is no information on the form of inhibin in testicular tissue. inhibin bioactivity was therefore estimated in eluates of slices, obtained after sds-page of rat testicular and ovarian homogenates, rat sertoli cell-conditioned medium (rsccm) and bovine ovarian follicular fluid (bff). the only form of inhibin detected in testes from 22-day-old rats and in rsccm was a 30 kda ...	1989	2504871
asparagine-linked glycosylation of the scrapie and cellular prion proteins.	post-translational modification of the scrapie prion protein (prp) is thought to account for the unusual features of this protein. molecular cloning of a prp cdna identified two potential asn-linked glycosylation sites. both the scrapie (prpsc) and cellular (prpc) isoforms were susceptible to digestion by peptide n-glycosidase f (pngase f) but resistant to endoglycosidase h as measured by migration in sodium dodecyl sulfate-polyacrylamide gel electrophoresis. pngase f digestion of prpc yielded t ...	1989	2505674
glycosylation characteristics of the mouse liver lactogenic receptor.	the structural characteristics and glycosylation properties of the lactogenic receptor were examined in 3-[(3-cholamidopropyl)dimethylammonio]-1-propane sulfonate (chaps)-solubilized plasma membranes from female mouse liver. the specific binding of the radioiodinated human growth hormone [( 125i]hgh) was displaced with an equivalent potency by both hgh and prolactin. after a mild neuraminidase treatment, this binding was increased by 40%, as a result of an increase in receptor affinity. affinity ...	1989	2506088
production of interferon-alpha and gamma by leukocytes from patients with schizophrenia.	production of interferon (ifn)-alpha and -gamma by leukocytes from 34 patients with acute schizophrenia and 34 controls was measured before and after 5-6 weeks of antipsychotic treatment by using sendai virus as ifn-alpha inducer and lentil lectin as inducer for ifn-gamma production. the schizophrenia series included 13 first admission patients (mean duration of illness 1.1 years) and 21 re-entry patients (mean duration of illness 10.1 years). of the total series 23 were drug-free at the time of ...	1989	2518635
molecular cloning and expression of cdna encoding the 53,000-dalton glycoprotein of rabbit skeletal muscle sarcoplasmic reticulum.	the 53-kda glycoprotein of rabbit skeletal muscle sarcoplasmic reticulum was purified by lentil lectin affinity chromatography and preparative polyacrylamide gel electrophoresis and partially sequenced. polyclonal and monoclonal antibodies were raised against the 53-kda glycoprotein and found to cross-react with the 160-kda glycoprotein. a combination of antibody and synthetic oligonucleotide screening was used to isolate a cdna encoding the 53-kda glycoprotein of rabbit fast-twitch skeletal mus ...	1989	2521635
isolation and characterization of complement receptors cr1 from human peripheral nerves.	extracts from myelinated and unmyelinated nerves, prepared using nonidet p-40, contained receptors for c3b/c4b (cr1). extracts from myelinated nerves inhibited eac3b rosette formation with peripheral blood leucocytes and agglutinated eac3b, whereas extract from unmyelinated nerves did not. rosette formation with eac3bi or eac3d was not affected. cr1 in extracts from myelinated nerves also expressed decay-accelerating activity of the alternative pathway c3 convertase and cofactor activity in fact ...	1989	2526822
primary structure of the major o-glycosidically linked carbohydrate unit of human von willebrand factor.	a reduced tetrasaccharide chain was obtained from human von willebrand factor (vwf) by mild alkaline borohydride treatment. the purification of this o-glycosidically-linked oligosaccharide was achieved by serial affinity chromatography on immobilized concanavalin a and lens culinaris agglutinin and finally gel filtration. its structure was determined by a combination of methylation studies and 500 mhz 1h-nmr spectroscopy to be: neuac(alpha 2-3)gal(beta 1-3)[neuac(alpha 2-6)]galnac-ol.	1989	2535488
production of antibodies against the coenzyme pyrrolequinoline quinone.	polyclonal antibodies against pyrrolequinoline quinone have been elicited in rabbits. these antibodies react with free and protein-bound pyrrolequinoline quinone. in particular they react with native and denatured lentil seedling amine oxidase as detected by dot-blot and elisa assays. the presence of 1 mol pyrrolequinoline quinone per mol of enzyme was determined by the last method.	1989	2541012
affinity cytochemical differentiation of glycoconjugates of small intestinal absorptive cells using pisum sativum and lens culinaris lectins.	we studied the subcellular localization of glycoconjugates recognized by the garden pea and lentil lectins (pisum sativum, psa; lens culinaris, lca) in mature absorptive cells of duodenum and jejunum of fasted rats. psa and lca are mannose-, glucose-, and n-acetyl-glucosamine-recognizing lectins that bind with high affinity to fucosylated core regions of n-glycosidically linked glycans. the binding reactions were cytochemically demonstrated in a pre-embedment incubation system using peroxidase-l ...	1989	2542394
a collagen-binding glycoprotein from bovine platelets is identical to propolypeptide of von willebrand factor.	several proteins from bovine platelet lysate bound to type i collagen immobilized to the beads of formyl derivatives of cellulose. among these proteins, a protein of about 100,000 daltons was purified to homogeneity by two additional affinity chromatographies, an organomercurial-agarose and a lentil lectin-agarose. this protein consisted of a single polypeptide chain which contains carbohydrate moiety and many intrapolypeptide disulfide bridges. in addition to platelets, this protein was present ...	1989	2543668
strong-cation-exchange sulfoethyl aspartamide chromatography for peptide mapping of staphylococcus aureus v8 protein digests.	in two recent reports (d. l. crimmins, j. gorka, r. s. thoma, and b. d. schwartz (1988) j. chromatogr. 443, 63-71; a. j. alpert and p. c. andrews (1988) j. chromatogr. 443, 85-96) a sulfoethyl aspartamide column was shown to efficiently analyze peptides less than 25 residues in length which differ in the number of nominal positive charges at ph 3.0. in particular, the elution order for a series of distinct peptides ranging in nominal charge from +1 to +7 was found to be monotonic in nature indic ...	1989	2545105
human gelatinase/type iv procollagenase is a regular plasma component.	gelatin zymograms revealed in human plasma a constant 66 kda proteolytically active polypeptide. in most plasma samples other major proteolytic activities were seen at mr 92,000, 130,000 and 225,000. all four proteases were ca2+-dependent metalloproteases and bound quantitatively to gelatin-sepharose. immunoblotting results indicated that the 66 kda protease was the human fibroblast gelatinase/type iv procollagenase and that the other three proteases were macrophage/granulocyte-derived gelatinas ...	1989	2551735
lectin binding to the porcine and human ileal receptor of intrinsic factor-cobalamin.	the purified porcine receptor for the intrinsic factor-cobalamin complex bound to concanavalin a, lentil lectin and wheat germ lectin covalently coupled to sepharose and was eluted with the corresponding soluble sugars. in contrast, human intrinsic factor bound efficiently to concanavalin a, to some extent to lentil lectin, but only slightly to wheat germ agglutinin. the binding of if-cbl to the receptor was inhibited when the receptor was pre-incubated with soluble wheat germ agglutinin, with a ...	1989	2562509
characterization of variant gamma-glutamyl transpeptidase produced by pancreatocholangiocarcinoma cell lines in a protein-free, chemically defined medium.	four pancreatocholangiocarcinoma cell lines (hpc-y1, hpc-yt, mia paca-2, and hchol-y1) were established to propagate in a protein-free, chemically defined medium. high gamma-glutamyl transpeptidase (ggtp) activities were showed in their spent media (designated as the secreted (ggtp). their ggtp activities in the spent media were 125, 85, 110, and 153 iu/l/mg of lyophilized spent media, whereas ggtp activities extracted from their cancer cell lines with bromelain were 105, 37, 86, and 112 iu/l/1 ...	1989	2569734
correlation between carbohydrate structures on the envelope glycoprotein gp120 of hiv-1 and hiv-2 and syncytium inhibition with lectins.	the binding of 13 different lectins to gp120 partially purified from two hiv-1 isolates and one hiv-2 isolate was studied by in situ staining on electrophoretically separated and electroblotted hiv antigens. the lectins concanavalin a, wheat germ agglutinin, lens culinaris agglutinin, vicia faba agglutinin, pisum sativum agglutinin and phytohaem(erythro)agglutinin bound to gp120 of all three isolates. the carbohydrate of gp120 recognized by lectins was thus arranged in at least four types of gly ...	1989	2574581
ultrastructure and lectin histochemistry of equine cutaneous histiolymphocytic lymphosarcomas.	tissues from subcutaneous lymphosarcomas and regional lymph nodes were examined by light and electron microscopy and by lectin histochemistry. tumors were composed of two major cell types: small lymphocytes with few organelles and pleomorphic histiocytic cells with undulant surfaces, large numbers of cytoplasmic vacuoles, and many mitochondria with large crystalline inclusions. a large gram-positive coryneform bacterium was isolated from tumor nodules but was not identified morphologically in tu ...	1989	2588437
developmental changes in proteins and glycoproteins revealed by direct radio-iodination of viable taenia saginata larvae.	direct surface 125i radio-isotope labelling techniques and sds-page analysis were used to compare the proteins and lentil-lectin adherent glycoproteins of the bovine stage of viable taenia saginata larvae at three points in their development, the invasive oncospheres, immature (4-week-old) and mature (12 to 16-week-old) cysticerci. some proteins and glycoproteins were present on all three of the ages of the parasite examined but there were also distinct age-specific proteins and glycoproteins de ...	1989	2594418
a detailed analysis of the potential of water-soluble classical class i mhc molecules for the suppression of kidney allograft rejection and in vitro cytotoxic t cell responses.	water-soluble classical (rt1-a) class i mhc molecules were purified from aqueous extracts of da strain liver. following monoclonal antibody affinity, lentil lectin affinity, and gel filtration chromatography, 600 micrograms of soluble rt1-a class i molecules with antigen activity equivalent to 1.3 x 10(11) nucleated da spleen cells (greater than 500 da spleens) was obtained. both pvg and lew strain recipients of da kidney allografts were pretreated with intravenous injections of the da soluble c ...	1989	2595764
a novel metalloproteinase associated with brain myelin membranes. isolation and characterization.	brain myelin membrane preparations contain a metalloproteinase activity which degrades myelin basic protein (mbp). the activity was associated with lentil lectin-binding glycoproteins solubilized from myelin and could be detected in the presence of the detergent 3-[(3-cholamidopropyl)dimethylammonio]-1-propane-sulfonate (chaps). the metalloproteinase represented about 5% of this glycoprotein fraction and was isolated from it by chromatography on deae-sephacel, cm-sepharose, and superose 6. the p ...	1989	2600084
cycle- and function-related changes in lectin binding to human endometrium: a histochemical study with pronase treatment.	forty-eight endometrial biopsy specimens were obtained during a normal menstrual cycle, during pregnancy and from patients with dysfunctional bleeding. the specimens were examined for peanut (pna), soybean (sba), vicia villosa (vva), phytohem- (pha), lens culinaris (lca) and concanavalin (succ. con a) agglutinin binding. the study was performed on paraffin sections using the pronase digestion and either the peroxidase-antiperoxidase or the avidin-biotin-peroxidase method. cycle-related changes o ...	1989	2619335
developmental changes in the glycosylation and binding properties of human fibronectins. characterization of the glycan structures and ligand binding of human fibronectins from adult plasma, cord blood and amniotic fluid.	fibronectins from human adult plasma, fetal plasma and from amniotic fluid obtained during early and late gestation were compared with respect to (i) their reactivity with lectins, (ii) their binding to the physiological ligands gelatin and heparin, and (iii) the role of the carbohydrate residues in the binding to these two ligands. the two fibronectin isoforms displayed distinct developmental differences in both glycosylation and binding properties: (i) proportions of tri/tetraantennary complex ...	1989	2619923