Publications

crush injury to peripheral nerve. an electron microscope study employing horseradish peroxidase.	electron microscope observations were made of rat peroneal nerve after crushing using intravenously injected horseradish peroxidase (hrp) as a tracer protein to indicate changes in vascular permeability. at 1/2 h and 2 d after the crush there was gross leakage of hrp from damaged capillaries at the site of injury but none from vessels above or below this. ultrastructurally vessels at the site of crush showed broken and separated endothelial cells. proximally and distally there was little abnorma ...	1976	970115
cells of origin of entorhinal cortical afferents to the hippocampus and fascia dentata of the rat.	the pathway from the entorhinal cortical region to the hippocampal formation has previously been shown to be comprised of two sub-systems, one of which projects predominantly to the ipsilateral fascia dentata and regio inferior of the hippocampus proper, and a second which projects bilaterally to regio superior. the goal of the present investigation was to determine if these two pathways might originate from different cell populations within the entorhinal area. the cells of origin of these ento ...	1976	972204
uptake and transport of exogenous proteins by respiratory epithelium.	the tracer proteins, horseradish peroxidase and ferritin, placed in the trachea of guinea pigs were taken up by epithelial cells and transported to the extracellular space. the interval between the introduction of the tracer proteins into the lumen of the trachea and the morphologic demonstration of the porteins in the extracellular space or within the basal portion of the cells was between 30 and 60 minutes. the proteins were transported in vesicles and no penetration of the epithelial intercel ...	1976	979161
reinnervation of dentate gyrus by homologous afferents following entorhinal cortical lesions in adult rats.	granule cells of the rat dentate gyrus which are denervated by unilateral destruction of the entorhinal cortex are reinnervated in part by proliferation of surviving pathways from the contralateral entorhinal cortex. the cells of origin of these lesion-induced projections were identified by retrograde labeling with horseradish peroxidase and were the same cell type which normally project to the ipsilateral dentate gyrus	1976	982024
ige-induced respiratory and circulatory changes during systemic anaphylaxis in the rabbit.	we studied systemic anaphylaxis induced by the administration of 200 mug of horseradish peroxidase into 11 anesthetized rabbits known to be producing anti-horseradish peroxidase antibodies only of the ige class. ventilatory changes included a transient, abrupt decrease in breathing frequency followed by increased minute ventilation; lung mechanical changes included decreased dynamic lung compliance and increased total pulmonary resistance; cardiovascular changes included pulmonary hypertension, ...	1976	984586
uptake of microperoxidase by segmenting rat ova in vitro.	uptake and incidence of microperoxidase (sigma) in the rat ova during cleavage at the 1-, 2-, 8-cell and blastocyst stages were studied after 30 min incubation with the enzyme (molecular weight of 1,900 and size of molecule of 2 nm). evidence was furnished of the presence of a small amount of reaction product of microperoxidase in the zona pellucida and its local concentration in some parts of the perivitelline space. the larger part of surface of the ovum, however, was free of microperoxidase. ...	1976	986379
quantitative ultrastructural autoradiographic studies of iodinated plasma membranes of lymphocytes during segregation and internalization of surface immunoglobulins.	rat spleen lymphocytes were iodinated (125 i) with lactoperoxidase. quantitative autoradiographic studies on cells fixed immediately after iodination showed 19-24% of intracytoplasmic grains at 3hd and over from the plasma membrane. normalization of grain density distribution and comparison of resulting curves with the universal curve of grain scatter of 125 i showed that a significant percentage of intracytoplasmic grains (36%) originates from intracytoplasmic labeled sources rather than from s ...	1976	986396
peroxidase-antiperoxidase complex. binding by mallory bodies in unfixed tissue.	an antigen-antibody complex of horseradish peroxidase, peroxidase-antiperoxidase (pap), was found to selectively bind to mallory bodies (mbs). specific pap binding to mbs was consistently demonstrated in liver sections from 14 patients with alcoholic cirrhosis and from one patient with hepatoma. mallory bodies in isolated fractions also bound pap. no structures stained with pap in six control sections. the pap-binding method is useful in the identification of mbs in situ and in isolated fraction ...	1976	989279
the use of tritiated horseradish peroxidase for defining neuronal pathways: a new application.		1976	990928
the precise localization of nigral afferents in the rat as determined by a retrograde tracing technique.	afferent innervation of the rat substantia nigra (sn) was studied by the retrograde horseradish peroxidase (hrp) method. high concentrations of hrp were deposited in discrete subregions of the sn by means of a microiontophoretic delivery system. using this technique it was possible to demonstrate that the caudatonigral projection system is arranged topographically; all portions of the caudate-putamen except for a central medial core were found to contain hrp positive cells, indicative of retrogr ...	1976	990939
axonal projections of spinal interneurones excited by group i afferents in the cat, revealed by intracellular staining with horseradish peroxidase.		1976	990947
intracellular staining of purkinje cells and their axons with horseradish peroxidase.		1976	990950
lymphocyte emigration from high endothelial venules in rat lymph nodes.	sequential events during lymphocyte emigration from high endothelial venuses (hev) were studied by scanning and transmission electron microscopy combined with regional perfusion techniques. the results indicate that blood lymphocytes selectively adhere to hev surfaces through microvilli which attach to shallow pits on the luminal surfaces of high endothelial cells. these intercellular contact points resist hydrodynamic and osmotic shearing forces, but can be disrupted by treatments which remove ...	1976	992709
pinocytic rates of macrophages from mice immunized against toxoplasma gondii and macrophages stimulated to inhibit toxoplasma in vitro.	the rate of pinocytosis by macrophages when measured by uptake of horseradish peroxidase was significantly reduced during toxoplasma infection of the cells in vitro when the macrophages were from toxoplasma-immune mice and when control cells were stimulated in vitro to inhibit toxoplasma multiplication. there was, however, no direct correlation between reduced pinocytosis in this model and inhibition or enhancement of toxoplasma multiplication. we conclude that a reduced pinocytic rate is a feat ...	1976	992866
a histochemical and morphologic study of postobstructive diuresis in the rat.	renal functional abnormalities constituting the syndrome of postobstructive diuresis imply both altered tubular and glomerular membrane properties. to determine the morphologic and ultrastructural correlates of this disorder a rat model was developed and 32 postobstructed kidneys were studied by light and electron microscopy at the midpoint of diuresis and compared to 22 controls. the abnormal morphology was: dilated distal tubules and collecting ducts, isolated proximal and distal tubule cells ...	1976	992966
time sequence and site of fluid accumulation in experimental neurogenic pulmonary edema.	the initial phase of pulmonary edema development following intracranial pressure elevation was studied by means of transmission electron microscopy. using perfusion fixation and application of a blood tracer (hrp horseradish peroxidase) the time sequence and site of fluid leakage out of pulmonary vessels was demonstrated: - passage of edema fluid through intercellular clefts of alveolar capillary endothelium - edema accumulation in alveolar interstitial tissue - draining of edema fluid from the ...	1976	996374
electron microscopic cytochemical localization of intravenously injected liposome-encapsulated horseradish peroxidase in rat liver cells.		1976	998408
kinetics of horseradish peroxidase migration through cerebral cortex.	this study provides a semiquantitative description of the migration of the extracellular space marker, horseradish peroxidase (hrp), through the cerebral cortex. following a continuous subarachnoid infusion of hrp, this marker was fixed rapidly within the cortical extracellular space (ecs) by intravascular aldehyde perfusion fixation. microscopic measurements of the maximum depth of penetration of hrp into the cortex perpendicular to the pial surface were taken from coronal whole brain sections ...	1976	1000288
cytochemical localization of glycoconjugate in mitochondria.	complex carbohydrates and cations have been localized by cytochemical methods in mitochondria of mammalian leukocytes, hepatocytes and oyster gill epithelium. glycoconjugate of acidic nature was visualized with the dialyzed iron method in or on the outer membrane and inner boundary membrane and in the outer intermembrane space but not on the membranes of cristae or in the intracristate space, or matrix. sulfated glycoconjugate was demonstrated with the high iron diamine method in a similar distr ...	1976	1002972
arterial hypotension induced by horseradish peroxidase in various rat strains.	the influence of various commercial preparations of horseradish peroxidase upon the arterial pressure has been investigated using direct intra-arterial pressure recordings in nembutal anesthetized rats of different strains. a marked hypotension was noted in wistar and sprague-dawley rats when sufficiently high doses (5-10 mg/100 g body weight) were injected intravenously. this effect was observed with both highly purified (sigma type vi, boehringer reinheitsgrad i) as well as less pure preparati ...	1976	1002975
cerebrovasculature permeability changes following experimental cerebral angiography. a light- and electron-microscopic study.	morphological alterations of the cerebral vasculature as related to the permeability of plasma proteins and angiographic contrast media following unilateral cerebral angiography were studied. both evans blue albumin and horseradish peroxidase were employed as protein tracers for light and electron microscopy investigation respectively. grey matter regions of the cerebral cortex, cerebellum corpus striatum, hippocampus and midbrain showed the most extensive and consistent leakage of these protein ...	1976	1003253
penetration of protein tracers into the epiretinal portion of the optic nerve in the rabbit nerve.	horseradish peroxidase and fluorescein-labelled globulin were injected into the vitreous of adult and 11- to 13-day-old new zealand albino rabbits. both tracers diffused rapidly into the myelinated nerve fibre layer (the "medullary rays"), which extends over a long distance within the retina in these animals. the tracers penetrated also for some distance into the optic nerve beyond the lamina cribosa, where they were incorporated into a large number of cells in the nerve. this finding of a rapid ...	1976	1003255
polysaccharides in lung alveoli.	rat lung alveolar surfaces and contents were studied after using concanavalin a as a bifunctional agent to link exposed sugars to horseradish peroxidase, in accordance with a technique developed by bernhard and avrameas ('71). the graham and karnovsky ('69) diaminobenzidine procedure then was used to provide and electron-dense reaction product so as to define the distribution of complex carbohydrates in alveoli. a layer of very dense raction product was intimately associated with the outer leafl ...	1976	1008038
horseradish peroxidase tracing of the lateral habenular-midbrain raphe nuclei connections in the rat.	connections of the habenular complex to the nuclei of the midline in the midbrain (interpeduncularis, medianus raphe, and dorsalis raphe) have been studied classically by anterograde degeneration in the monkey, the cat, and marsupials. passing fibers from the medial septal nucleus and lateral preoptic area, however, have also been demonstrated which can complicate interpretation of these results. in this paper the habenular projections were studied in the rat by the retrograde axonal transport o ...	1976	1009445
thalamic projections to visually responsive regions of parietal cortex.	thalamic efferents to visually responsive regions of parietal cortex of the cat were investigated by experimental retrograde tracing techniques. two classes of photically evoked potentials recorded from the middle suprasylvian gyrus could be distinguished on the basis of waveform and latency. type 1 responses were recorded from the clare-bishop area at the lateral border of the suprasylvian gyrus and type 2 responses were recorded from association response areas on the crown of the suprasylvian ...	1976	1009447
the development of the isthmo-optic nucleus.	the nucleus of origin of centrifugal fibers to the retina (the so-called isthmo-optic nucleus - ion) has been used as a model for the study of the major features of neural development, from the period of cell proliferation until after the formation of its afferent and efferent connections. 3h-thymidine autoradiography has established that in the chick cells of the ion are generated (i.e., become post-mitotic) between the middle of the 5th and the end of the 7th days of incubation. the first-form ...	1976	1016847
[ultrastructural studies on the permeability of the amnion-epithelium for peroxidase and ferritin. contribution on paraplacental metabolism].	by means tracer substances (horseradish peroxidase, ferritin) the permeability of human amnion and umbilical cord (2. to 6. lunar month) has been investigated ultrastructurally. the structure of intercellular cleft permits a passiv transfer of the water and electrolytes in both ways. the findings indicated, that the passage of proteins with a molecular weight of peroxidase predominantly takes place through the intercellular clefts less by means of pinocytosis. ferritin particles can not penetrat ...	1976	1020513
[origin of afferent axons in the striate cortex of the albino rat: a study with horseradish peroxidase].		1976	1031505
coronary microcirculatory factors and cardiac muscle cell injury.	coronary artery ligation with or without reperfusion was carried out in wistar rats to study the role of coronary microcirculatory factors and membrane permeability alteration of cardiac muscle cell in the evolution of cardiac muscle cell injury by using the fine structural extracellular protein tracer, horseradish peroxidase (hrp). the findings were compared with those obtained in noncoronarogenic myocardial injury models following administration of norepinephrine, a pressor, and isoproterenol, ...	1976	1032011
the relationship between storage and secretion of specific antibody by immune lymphoid cells: ultrastructural localization of anti-peroxidase antibodies in plaque-forming cells of the rabbit popliteal lymph node.	the ultrastructure of antibody-forming cells (afc) has been studied in the lymph node cell population from rabbits locally immunized with horseradish peroxydase (po) incorporated in complete freund's adjuvant, and the kinetics of afc development followed from day 7 to day 18 after one injection of po. identification of the afc was done by local hemolysis assay, using carboxymethyl cellulose solidifying medium and po-coated sheep erythrocytes. afc were thereafter transferred by micromanipulation ...	1976	1033066
organization of thalamocortical auditory system in the cat studied with horseradish peroxidase.		1976	1244977
peroxidase activity of alveolar macrophages.	peroxidase activity was studied in alveolar macrophages and compared to the peroxidase activity in polymorphonuclear leukocytes using cytochemical techniques. a dense reaction product for peroxidase was observed in the primary lysosomes of polymorphonuclear leukocytes, but no significant peroxidase or peroxidative enzymes could be detected in rabbit alveolar macrophages. furthermore, following vigorous phagocytosis of zymosan particles by alveolar macrophages in vitro, no peroxidase could be det ...	1976	1246123
the penetration of fluorescein-conjugated and electrondense tracer proteins into xenopus tadpole optic nerves following perineural injection.	the permeability of xenopus tadpole optic nerves to macromolecules was studied in order to evaluate the usefulness of this system for studying mechanisms of serum-induced cns demyelination in vivo. single injections of either horseradish peroxidase (hrp), ferritin or fluorescein-conjugated human igg were injected around the right optic nerve and tadpoles were then sacrificed between 15 min and 48 h. each of the tracers had penetrated the nerve parenchyma by 30 min. entry of hrp and ferritin occu ...	1976	1247883
[experience with the use of horseradish peroxidase for light and electron microscope studies of interneuronal connections].	by the method based on a retrograde axonal transport of exogenous horseradish peroxidase (hrp), the origins of afferentation of the motor cortex of adult cats, kittens and albino rats were studied. hrp-positive neurons were found by light and electron microscopy in the somatosensory cortex (c1) of the ipsilateral hemisphere and in the portions of the cortex of the contralateral hemisphere which were symmetrical to the site of injection of hrp. the disposition of neurons, marked by hrp, in the vt ...	1976	1252130
simultaneous visualization by light microscopy of two pituitary hormones in a single tissue section using a combination of indirect immunohistochemical methods.	two indirect methods involving enzyme-labeled antibodies were used to demonstrate simultaneously two distinct tissue antigens in the same histologic section without a need for antigen-antibody dissociative procedures. sections of rat pituitary gland were incubated with rabbit anti-rat luteinizing hormone followed by goat anti-rabbit gamma-globulin conjugated to horseradish peroxidase. the same sections were then further incubated with monkey anti-rat growth hormone followed by goat anti-monkey g ...	1976	1254941
intracellular application of horseradish peroxidase and its light and electron microscopical appearance in spinocervical tract cells.		1976	1260464
ultrastructural studies of mouse leucocytes showing surface binding of peroxidase-conjugated lectins.	examination in the electron microscope of mouse spleen cells treated with conjugates of wheatgerm agglutinin and horseradish peroxidase has shown the surface of myeloid cells to be heavily stained. resting lymphocytes were unstained but became positive after stimulation by phytohaemagglutinin or concanavalin a, suggesting a relationship between the presence of this surface component and the functional state of the cell and providing a further point of similarity between myeloid and lymphoblastoi ...	1976	1261094
afferents to the cerebellar lateral nucleus. evidence from retrograde transport of horseradish peroxidase after pressure injections through micropipettes.	hrp was injected by pressure from glass capillary micropipettes unilaterally into the lateral nucleus of rat so as to encompass the entire nucleus, but without spread into the interpositus nuclei. the cells of origin of the afferents to the lateral nucleus were studied after retrograde transport of the hrp. the reticulotegmental nucleus of the pons was labelled bilaterally and is the major source of crossed and uncrossed reticular imputs. the pontine nuclei also provide extensive crossed and unc ...	1976	1262557
glomerular permeability to proteins. effects of hemodynamic factors on the distribution of endogenous immunoglobulin g and exogenous catalase in the rat glomerulus.	the distribution of endogenous immunoglobulin g (igg) and exogenous catalase was delineated in the rat glomerulus under normal and abnormal hemodynamic conditions. igg was identified by an ultrastructural immunoperoxidase technique using antirat igg fab fragments conjugated to horseradish peroxidase; catalase was identified by a cytochemical reaction. when superficial glomeruli in anesthetized munich-wistar rats were rapidly fixed in situ by dripping glutaraldehyde onto the renal surface, igg an ...	1976	1263444
transendothelial vesicular transport of protein following compression injury to the spinal cord.	routes of vascular leakage resulting in trauma-induced edema have not been clarified. to explore the problem we followed the fate of intravascular horseradish peroxidase (hrp) after compression injury to the thoracic cord (cats). at 90 seconds and 15 minutes, hrp was confined to the gray matter, occupying perivascular spaces, unexpanded extracellular channels, and cytoplasmic compartments of injured cells. adjoining segments contained similar but lesser deposits. at 4 hours, tracer occupied the ...	1976	1263445
acute hypersensitivity pneumonitis in the rabbit. i. an animal model with horseradish peroxidase as antigen.	an experimental model of acute hypersensitivity pneumonitis was developed in rabbits using horseradish peroxidase as antigen. the lesion, produced by aerosol challenge with horseradish peroxidase 21 days after a single parenteral immunization with horseradish peroxidase and complete freund adjuvant, was characterized by diffuse infiltration of respiratory bronchioles and of alveolar ducts and walls with approxomately equal numbers of mononuclear cells (lymphocytes and macrophages) and polymorph ...	1976	1267257
immunohistochemical localization of lh-producing cells in the adenohypophysis of the japanese quail, coturnix coturnix japonica.	the cells that produce luteinizing hormone (lh) in the adenohypophysis of the japanese quail were identified immunohistochemically using anti-chicken lh serum and horseradish peroxidase-labeled goat anti-rabbit gamma globulin serum. the lh cells are localized in the caudal lobe of the pars distalis. they are elongate in shape shape and are polarized toward the sinusoids, especially in their active states. alterations in size of lh cells are directly related to changes in circulating lh levels as ...	1976	1268921
nmr studies of hemoproteins: ph dependence of ferric horseradish peroxidase and horse heart myoglobin.		1976	1269749
the structure of lymphatic capillaries in lymph formation.	the lymphatic vascular system consists of endothelial lined vessels which begin as blind-end tubes or saccules that are located within the connective tissue areas. this system serves as a one-way drainage apparatus for the removal of diffusible substances as well as plasma proteins that escape the blood capillaries. if permitted to accumulate, these escaped components would deplete the circulatory system of its plasma colloids and disrupt the balance of forces responsible for the control of flui ...	1976	1269772
projections of the locus coeruleus and adjacent pontine tegmentum in the cat.	the projections of the locus coeruleus and adjacent pontine tegmentum have been studied using anatomical and physiological methods in the cat. axonal trajectories were traced using either the fink-heimer i method following electrolytic lesions, or the autoradiographic method after injection of tritiated proline into the nucleus. results with both methods were similar. axons of locus noeruleus neurons ascended ipsilaterally through the mesencephalon lateral to the medial longitudinal fasiculus, v ...	1976	1270609
convergent thalamic and mesencephalic projections to the anterior medial cortex in the rat.	small microelectrophoretic deposits of horseradish-peroxidase (hrp) were placed at various loci within the gray matter of the rat's anterior medial cortex. a comparison of the patterns of retrograde cell-labeling charted in 26 such cases confirmed earlier findings in fiber-degeneration studies according to which the respective thalamocortical projections of the mediodorsal (md) and anteromedial nucleus (am) overlap each other over a wide region of the anterior medial cortex. this region of thala ...	1976	1270614
the olivocerebellar projection in the cat as studied with the method of retrograde axonal transport of horseradish peroxidase. ii. the projection to the uvula.	following injections of small quantities of horseradish peroxidase (hrp) suspension in the uvula of the cat, the distribution of labeled cells in the inferior olive has been mapped. the findings confirm the conclusion made on the basis of studies of retrograde cell loss in the olive following ablations of the uvula (brodal, '40b) that two small olivary subdivisions, the nucleus beta and the dorsomedial cell column project heavily to the uvula. in addition the hrp-study shows that the uvula recei ...	1976	1270615
scanning electron microscopy of the subarachnoid space in the dog. iv. subarachnoid macrophages.	young dogs of both sexes were used in this study. transmission and scanning electron microscopy were utilized for the examination of the spinal cord and choroid plexus with emphasis on the study of free cells. these procedures were modified so that, in certain cases, the same cells observed in scanning electron microscopy could be analyzed internally by transmission electron microscopy. one half of the animals were injected under anesthesia with horseradish peroxidase for observation of phagocyt ...	1976	1270624
development of an enzyme immunoassay for human placental lactogen using labelled antibodies.	an immunoassay for the determination of human placental lactogen (hpl) has been developed using specific antibodies labelled with horseradish peroxidase. a method is presented in which the enzyme : antibody conjugate is purified with an antigenic solid phase to ensure the production of conjugates with intact immunological activity. serum components interfere with the assay but this problem can only be avoided at the expense of a precise and practical assay for the determination of hpl.	1976	1270811
permeability alteration of sarcolemmal membrane in catecholamine-induced cardiac muscle cell injury. in vivo studies with fine structural diffusion tracer horse radish peroxidase.	cardiac muscle cell injury was produced in male sprague-dawley rats weighing 300 to 450 gm. with catecholamines, norepinephrine, and isoproterenol; sarcolemmal membrane alteration was tested in vivo using the extracellular macromolecular tracer, horseradish peroxidase. norepinephrine was administered in continuous intravenous infusion in a dose of 4 to 6 mug. per 100 gm. of body weight per minute, whereas isoproterenol was given as a single subcutaneous injection in a dose of 8.5 mg. per 100 gm. ...	1976	1271749
endocytosis by vascular smooth muscle cells in vivo and in vitro. roles or vesicles and lysosomes.	overloading of lysosomes of smooth muscle cells with excess substrate may be a key event in the development of hypertensive and atherosclerotic vascular disease. cellular uptake of materials and its relation to lysosomal function were studied by ultrastructural cytochemistry in aortic smooth muscle cells grown in vitro and in the intact animal. injection of horseradish peroxidase (hrp) into hypertensive rats resulted in rapid insudation of the material into the environs of medial smooth muscle c ...	1976	1275059
direct projections from hypothalamus to hippocampus in the rat demonstrated by retrograde transport of horseradish peroxidase.		1976	1276883
an improved technique for the microinjection of horseradish peroxidase.		1976	1276885
changes in the distribution of the dentate gyrus associational system following unilateral or bilateral entorhinal lesions in the adult rat.	the distribution of the dentate gyrus associational system was analyzed in naive adult rats and in those with either unilateral or bilateral lesions of the entorhinal cortex. horseradish peroxidase histochemistry was used to trace the origin and course of this intrinsic fiber system. the fibers originated in the ca3-4 pyramidal cell field, apparently medial to the origin of the schaffer collateral system, and followed a trajectory which was essentially identical to that described for this system ...	1976	1276951
ultrastructure and permeability of lymph node microvasculature in the mouse.	the microvasculature of lymph nodes and peyer's patches consists of arterioles, capillaries and venules. the postcapillary segment comprises high-endothelial venules (he venules) as well as ordinary venules. in order to study the ultrastructure of the microvasculature, particularly with respect to the nature of intercellular junctions, lanthanum and ruthenium red were used as tracers. furthermore, to evaluate the permeability properties of the different segments of the microvasculature, intraven ...	1976	1277282
cooperative binding of concanavalin a to thymocytes at 4 degrees c and micro-redistribution of concanavalin a receptors.	the mode of binding of 125i-labelled concanavalin a and succinyl-concanavalin a to rat thymocytes at 4 degrees c was investigated. simultaneously, the free binding sites of the cell-bound lectin molecules were quantified by horseradish peroxidase binding. concanavalin a showed cooperative binding while succinyl-concanavalin a did not. the number of molecules of concanavalin a bound to the cell surface when it was saturated was twice the number of molecules of succinyl-concanavalin a. we interpre ...	1976	1278183
[afferent projections to the locus coeruleus nucleus in the cat. study by the horseradish peroxidase technic].	using a retrograde tracer technique with horseradish peroxidase, we have revealed some afferent projections to the locus coeruleus complex from the contralateral pontine tegmentum, raphe nuclei, substantia nigra, nucleus of the solitory tract, dorsal motor nucleus of the vagus and other regions of the ponto-bulbar reticular formation as well as from hypothalamic and preoptic areas.	1976	134760
distribution of immune deposits in the skin of reversed passive arthus reaction using horseradish peroxidase as antigen.		1976	45717
cellular localization of cyclic amp in periodontal tissues during experimental tooth movement in cats.	using an immune-histochemical method, cyclic amp was localized in cells of periodontal tissues in orthodontically-treated cats. sixteen cats were treated for periods ranging from 1 hour to 4 weeks. fresh, frozen, undecalcified 6 mu sections of the tissues were incubated with rabbit anti-cyclic amp antibodies, followed by sequential incubations with sheep anti-rabbit igg, rabbit anti-peroxidase igg and horseradish peroxidase. in the final step, the peroxidase was demonstrated by the diaminobenzid ...	1976	175896
[further purification and immunohistological localization of human placental lactogen in syncytiotrophoblasts (author's transl)].	in 1936, ehrhadt reported a prolactin-like substance which has been identified as a second protein hormone exists in extracted substance from the placenta. to date various reports have been published, but ther are still unsolved problems concerning the specifity of the substance, and its clinical significance is unclear. the purpose of this project was to purify and investigate the biochemical, immunological properties of hpl, and try to localize hpl secretion immunohistologically. the biologica ...	1976	176070
studies on the chlorinating activity of myeloperoxidase.	two methods were utilized to demonstrate the peroxidation of chloride ion to a free species (hocl or cl2) by myeloperoxidase. the peroxidase caused the volatilization of radioactivity from soultions containing hydrogen peroxide and [36cl]nacl, and catalyzed the formation of hocl when solutions contianing these components were passed through a millipore filter to which the peroxidase was adsorbed. in this flow system, 90 mug of canine myeloperoxidase generated 80 mum hocl in the presence of 200 m ...	1976	176150
use of horseradish peroxidase to study the antagonism of clostridium welchii (cl. perfringens) type d epsilon toxin in mice by the formalinized epsilon prototoxin.		1976	176185
subcellular localization of b apoprotein of plasma lipoproteins in rat liver.	multispecific antigen-binding fragments (fab) from rabbit antisera against rat very low density lipoproteins (vldl) and fab against rat low density lipoproteins that were monospecific for the b apoprotein were conjugated to horseradish peroxidase. conjugates were incubated with 6-mum frozen sections from fresh and perfusion-fixed livers and with tissue chopper sections (40 mum thick) from perfusion-fixed livers. in the light microscope, specific reaction product was present in all hepatocytes of ...	1976	177430
peroxidase-conjugate chromatography isolation of conjugates prepared with glutaraldehyde or periodate using polyacrylamide-agarose gel.	fractionation abilities of polyacrylamide-agarose gel (ultrogel) and dextran gel (sephadex) column chromatography were compared in isolating horseradish peroxidase conjugates, prepared using two different methods. utrogel aca-44 provides an efficient separation of monomer conjugated and nonconjugated immunoglobulins resulting from the two-step glutaraldehyde procedure, sephadex g-200 does not. both types of columns eluted the polymer conjugates resulting from the periodate procedure in the void ...	1976	177698
secretion-related uptake of horseradish peroxidase in neurohypophysial axons.	during secretion of the neurohypophysial hormones, oxytocin and vasopressin, secretory granule membrane is added to the plasma membrane of the axon terminals. it is generally assumed that subsequent internalization of this additional membrane occurs by endocytosis. in order to study this process, we have traced the uptake of intravenously injected horseradish peroxidase by neurohypophysial axons in rats and golden hamsters. peroxidase reaction product within the secretory axons was found mainly ...	1976	181385
direct immunoperoxidase staining for regan isoenzyme of alkaline phosphatase in human tumor tissues.	immunoperoxidase staining for regan isoenzyme of alkaline phosphatase was performed on cryostat sections of five human tumor tisssues. with a direct immunoperoxidase staining for the localization of regan isoenzyme at the light and electron microscope levels, sections previously fixed with 0.05 m phosphate-buffered 4% paraformaldehyde were reacted with rabbit antisera to human placenta alkaline phosphatase conjugated to horseradish peroxidase. comparison of conventional histochemistry and immuno ...	1976	182352
early events in polyoma virus infection: attachment, penetration, and nuclear entry.	the plaque-assay technique was used as a tool to determine the optimal conditions for adsorption of polyoma virions to host cells. using these optimal conditions of adsorption, an electron microscopy study of the early events of infection was performed. by electron microscopy and autoradiography, it was demonstrated that both the viral coat proteins and dna arrive simultaneously in the nucleus as early as 15 min postinfection. when horseradish peroxidase-labeled virions, pseudovirions, and capsi ...	1976	183018
peroxidase and fluorescein isothiocyanate as antibody markers. a quantitative comparison of two peroxidase conjugates prepared with glutaraldehyde or periodate anda fluorescein conjugate.	batches of rabbit anti-human immunoglobulin g antibodies were labeled either with horseradish peroxidase, using the two-step glutaraldehyde method or the periodate method, or with fluorescein isothiocyanate (fitc). the peroxidase conjugates were isolated by chromatography using two different gel types. the five types of conjugates thus obtained were standardized to the same amount of rabbit immunoglobulin g. the antibody activity, as estimated by means of single radial immunodiffusion and passiv ...	1976	184204
beneficial effect of catalase treatment on growth of clostridium perfringens.	several common plating media were tested for their ability to support growth of clostridium perfringens after storage of the plates for 1 to 10 days at 4 and 25 degrees c with and without subsequent addition of catalase. liver-veal (lv) agar and brain heart infusion (bhi) agar quickly become incapable of supporting growth after storage without added catalase, whereas shahidi ferguson perfringens (sfp) agar and brewer anaerobic (ba) agar were less affected. plate counts of c. perfringens on untre ...	1976	185958
tracing axons and axon collaterals of spinal neurons using intracellular injection of horseradish peroxidase.	intracellular injection and subsequent histochemical localization of horseradish peroxidase have been used to stain the soma, dendrites, axons, and axon collaterals of spinalcervical tract neurons and unidentified dorsal horn neurons in the cat. this technique may be used in combination with the intracellular injection of procion yellow to demonstrate by light microscopy connections between physiologically typed vertebrate neurons.	1976	54936
factors affecting retrograde axonal transport of horseradish peroxidase in the visual system.		1976	55291
light and electron microscopic observations on the anterograde transport of horseradish peroxidase in the optic pathway in the mouse and rat.		1976	55292
electron microscopic immunohistochemical identification of endothelial cells in the rabbit.	antibody to tissue factor apoprotein was adsorbed against gamma-globulin and coupled to horseradish peroxidase; this complex was applied to various rabbit tissues. the distribution of the peroxidase marker then was observed by electron microscopy. we examined fixed or frozen sections, as well as breis of aorta, vena cava, brain, heart, lung, liver, spleen, kidney, bone marrow, mesothelial gut lining, erythrocytes, and platelets. all endothelial cells that had been exposed to the antibody complex ...	1976	55315
retrograde transport of horseradish peroxidase in transected axons. 3. entry into injured axons and subsequent localization in perikaryon.	horseradish peroxidase (hrp) applied to crushed mouse sciatic nerves diffused through the damaged perineurium into the endoneurium. in the injured area, hrp passed into damaged myelinated and unmyelinated axons forming columns of reaction product, which extended for several millimeters proximally to the lesion. ultrastructurally, hrp adhered to the inner surface of the axoplasm and to the surfaces of neurotubules and neurofilaments in such columns. at more proximal levels axons contained hrp in ...	1976	61782
afferents to the rat substantia nigra studied with horseradish peroxidase, with special reference to fibres from the subthalamic nucleus.		1976	61785
a horseradish peroxidase study of the cortico-olivary projection in the cat.		1976	61790
direct projections from the mesodiencephalic midline areas to the pericruciate cortex in the cat: an experimental study with the horseradish peroxidase method.		1976	61792
nigrothalamic projections in the rat as demonstrated by orthograde and retrograde tracing echniques.	the nigrothalamic projection of the rat was investigated by means of horseradish peroxidase (hrp) histochemistry and by the autoradiographic tracing technique. the use of these techniques has permitted an extension of previous descriptions by identifying a neuron population in the substantia nigra, pars reticulata (snr) as the nigrothalamic cells of origin. these calls (20-25 mu) were multipolar and were distributed throughout the snr. there was a tendency however for more labelled neurons to be ...	1976	61793
origins of axons in the cat's acoustic striae determined by injection of horseradish peroxidase into severed tracts.	origins and terminations of fibers of the dorsal and intermediate acoustic striae were studied by surgically severing these tracts and injecting hrp into the incision. this procedure results in filling the severed axons with hrp. filled axons were traced to cell groups of origin and to some terminations of the acoustic striae. hrp-labeled terminals were found in the cochlear nuclei as well as in periolivary cell groups. filling of cells with hrp ranged from being complete, resulting in golgi-lik ...	1976	61976
the origin of the pretecto-olivary tract. a study using the horseradish peroxidase method.		1976	62601
olfactory relationships of the telencephalon and diencephalon in the rabbit. iii. the ipsilateral centrifugal fibers to the olfactory bulbar and retrobulbar formations.	the axoplasmic retrograde transport of horseradish peroxidase (hrp) from axon terminals to their parent cell bodies and histochemical fluorescence microscopy have been used to study the ipsilateral centrifugal fibers to the olfactory bulbs and anterior olfactory nucleus in the rabbit. focal injections of peroxidase were placed unilaterally into the main or accessory olfactory bulb or into the anterior olfactory nucleus. in animals with injected hrp confined within the main bulb, perikarya retrog ...	1976	62770
the use of glutaraldehyde-conjugated horseradish peroxidase-bovine serum albumin in the visualization of concanavalin a binding to tissue sections of human colonic tumor.	a method is reported for the preparation of glutaraldehyde cross-linked horseradish peroxidase conjugates for the visualization of concanavalin a binding to frozen sections. the binding of concanavalin a to tissue sections of human colonic tumor is used to compare staining by horseradish peroxidase conjugates and monomers. without decreasing specificity, the use of progressively larger molecular weight conjugates provides progressively greater intensity of staining of the epithelial components o ...	1976	62892
critical factors involved in the demonstration of horseradish peroxidase retrograde transport.		1976	56209
projection of thalamic neurons to cat primary vestibular cortical fields studied by means of retrograde axonal transport of horseradish peroxidase.	the two vestibular cortical projection areas in the anterior suprasylvian sulcus and post-cruciate dimple regions were defined by evoked potential technique in anaesthetized cats. the thalamic location of neurons with axon terminals in these fields was determined using the method of retrograde axonal transport of horseradish peroxidase. the ascending vestibular pathway appeared to be separated also at the thalamic level, where cells in the ventroposterolateral nucleus were found to project to th ...	1976	56281
ultrastructural differences between the preterminal nerve fibres and their endings in the mechanoreceptors, with special reference to their degeneration and mode of uptake of horseradish peroxidase.		1976	56762
localization of rabbit gamma globulins in the mouse visceral yolk sac placenta.	the yolk sac placenta has been implicated previously in transmission of passive immunity to the fetus. this work uses an immunohistochemical technique devised by sternberger et al. ('69) to study this problem. rabbit serum containing gamma globulins was injected into the uterine lumen of the white swiss mouse during the last third of pregnancy. two or four hours later yolk sacs were removed and fixed in 2% paraformaldehyde or freeze-dried and fixed with paraformaldehyde vapors. finely chopped ti ...	1976	56909
a note on the method of retrograde transport of horseradish peroxidase as a tool in studies of afferent cerebellar connections, particularly those from the inferior olive; with comments on the orthograde transport in purkinje cell axons.	1. injections of horseradish peroxidase (hrp) suspension were made in the cerebellar cortex of cats (most often the paramedian lobule). the staining of the cerebellar cortex and the ensuing labeling of neurons in the inferior olive were studied in experiments with variations of concentration of hrp, amounts of fluid injected, survival time and age of the animals. light microscopical studies were supplemented with electron microscopical observations. the folded cerebellar cortex offers particular ...	1976	57065
movement of horseradish peroxidase in rabbit submandibular glands after ductal injection.	horseradish peroxidase (hrp) has been used as a tracer to study movements of solutions injected retrogradely via the duct of submandibular glands in rabbits. 0.1 ml of solution was injected either manually or by a constant hydrostatic pressure, and the subsequent distribution of hrp in the gland and duct at different times after injection has been examined histochemically at light and electron microscopical levels. shortly after the injections, strong interstitial staining for peroxidase resulte ...	1976	57956
the development of the isthmo-optic tract in the chick, with special reference to the occurrence and correction of developmental errors in the location and connections of isthmo-optic neurons.	the development of the centrifugal projection to the chick retina in the isthmo-optic tract (iot) has been studied by the retrograde transport of the enzyme marker horseradish peroxidase (hrp) injected into the eye at various times during the incubation period. some neurons in the isthmo-optic nucleus (ion--the cells of origin of the iot) can first be labeled from the eye following injections on the tenth day of incubation; after injections on the twelfth day or later, about 95% of the neurons c ...	1976	58875
a guide to the synaptic analysis of the neuropil.	a morphological analysis of the organization of the gray matter in the central nervous system depends on the discovery of consistent repetitive patterns. without these, the gray matter remains a chaotic jungle. an hypothesis derived from the study of a few simple regions has been developed to serve as a guide in finding these patterns. it states that all nerve fibers and terminals arising from a particular group of nerve cells, or, more precisely, a particular nerve cell type, display similar ax ...	1976	59655
anti-streptococcal antibodies reacting with brain tissue. ii. utrastructural studies.	the reaction of rabbit anti-streptococcus pyogenes type 24 with mouse brain has been studied at the ultrastructural level using horseradish peroxidase labelled antiglobulin. it is shown that these antibodies, and by inference those found in sydenham's chorea, react with the fibrous astrocyte. the cross-reacting antigen is the glial filament or a closely associated cytoplasmic constituent.	1976	60120
differences between alpha and gamma motoneurons labeled with horseradish peroxidase by retrograde transport.		1976	60166
immune complex receptors on cell surface. i. ultrastructural demonstration of macrophages.	a method is described for ultrastructural localization of immune complex receptors on the surface of viable peritoneal exudate cells. the technique entails incubation with a soluble complex of horseradish peroxidase (hrp) and specific antibody to hrp at 4 degrees c followed by exposure to diaminobenzidine and processing for electron microscopy. the bound immune complexes were evident as focal deposits of hrp reaction product, adhering closely to the external surface of macrophages with an uninte ...	1976	60441
the olivocerebellar projection in the cat studied with the method of retrograde axonal transport of horseradish peroxidase. iii. the projection to the vermal visual area.	horseradish peroxidase (hrp) was injected separately in one of the cerebellar lobules vi, viia, viib, viiia or viiib (together corresponding to the vermal visual area) in 17 cats. after 1-3 days the distribution of labeled cells in the inferior olive was mapped. in spite of some overlapping it is clear that the various lobules of the vermal visual area receive fibers from separate parts of a horseshoeshaped region in the caudal half of the contralateral medial accessory olive (fig. 5c). the proj ...	1976	61211
comparative immunoenzymatic localization of prolactin and growth hormone in human and rat pituitaries.	twelve human and twelve rat pituitaries were stained by an immunohistochemical method using a rabbit anti-ovine prolactin serum, a rabbit anti-human growth hormone serum and a sheep anti-rabbit immunoglobulin serum conjugated with horseradish peroxidase. on the same pituitary section, growth hormone cells were stained brown by using 3-3'-diaminobenzidine as peroxidase substrate, and prolactin cells were stained purplish blue by using 4-chloro-1-naphtol. growth hormone cells outnumbered prolactin ...	1976	61240
prenatal development of central optic pathways in albino rats.	the development of the central optic projections in albino rat fetuses has been studied using light and electron microscopic degeneration techniques and the horseradish peroxidase method for demonstrating axonal projections of neurons. the first optic axons to reach the region of the optic chiasm arrive at day 15. by day 16, a substantial optic chiasm is seen and the optic tract can be traced into the epithalamus, having first passed through the ventral lateral geniculate nucleus and a thin lami ...	1976	54370
the climbing fibers of the cerebellar cortex, their origin and pathways in cat.	the sources and pathways of the climbing fibers to the cerebellar posterior vermis were studied with comibined electrophysiological and anatomical methods in cats. recording from identified cerebellar purkinje cells, monosynaptic climbing fiber (cf) responses have been obtained both for stimulation of the inferior olive (io) and various parts of the brain stem (bs). cf responses were found to of three types, io only, bs only or both io and bs. however the responses to bs stimulation were very fe ...	1976	63384
horseradish peroxidase: an improvement in intracellular staining of single, electrophysiologically characterized neurons.		1976	63386
the anatomical organization of the cerebello-olivary projection in the cat.	the cerebello-olivary pathway in the cat has been examined using orthograde and retrograde neuroanatomical tracing techniques. the orthograde transport of 3h-leucine from injection sites in the deep cerebellar nuclei labeled dentate and interpositus projections to the rostral two-thirds of the contralateral inferior olivary complex. these projections are topographically organized, with the dentate nucleus projecting to the principal olivary nucleus and the posterior and anterior interpositus nuc ...	1976	63472
the blue reaction product in horseradish peroxidase neurohistochemistry: incubation parameters and visibility.	a blue reaction product is formed at sites that contain horseradish peroxidase (hrp) activity when benzidene is used as the chromogen. with neutral red as a counter stain, this method affords excellent visualization of both retrograde and orthograde axonal transport of intracerebrally injected hrp. the visibility of this blue reaction-product is better than the visibility of the brown reaction-product obtained in the commonly used diaminobenzidene procedures. variations in incubation times and r ...	1976	63512