Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| [tagging and mapping of qtls controlling lint yield and yield components in upland cotton (gossypium hirsutum l.) using ssr and rapd markers]. | using interval mapping and marker simple regression methods, the qtls of yield and its components in (simian 3 x tm-1) f2 and f2:3, were tagged and mapped with 39 ssr and 10 rapd markers having polymorphism between parents screened from 301 pair ssr primers and 1040 rapd primers. simian 3 is being grown extensively in yangtze river cotton-growing valley characterized as high productivity with more bolls and higher lint percent, whereas tm-1, genetic standard in upland cotton with more heavy boll ... | 2002 | 12148276 |
| effects of kaolin-based particle film application on boll weevil (coleoptera: curculionidae) injury to cotton. | this study examined a non-insecticidal tactic for suppressing boll weevil, anthonomus grandis grandis boheman, damage to cotton, gossypium hirsutum l. in cage assays, kaolin, a reflective white mineral, applied to excised cotton squares or to the cotton foliage, initially resulted in lower levels of boll weevil injury to squares than nontreated squares. boll weevil oviposition and feeding on kaolin-treated squares and squares on kaolin-treated cotton plants increased when nontreated squares and ... | 2002 | 12216817 |
| survival of the rhizosphere-competent biocontrol strain pseudomonas fluorescens nbri2650 in the soil and phytosphere. | pseudomonas fluorescens nbri2650 was isolated after screening 360 bacterial strains from the rhizosphere of chickpea (cicer arietinum l.) grown in fungal-disease-suppressive field soil. the strain was selected because of its high rhizosphere competence and ability to inhibit the growth of fusarium oxysporum f.sp. ciceri, rhizoctonia bataticola, and pythium sp. under in vitro conditions. survival and colonization of nbri2650 in the phytosphere of chickpea, cotton (gossypium hirsutum l.), cucumber ... | 2002 | 12224558 |
| the bifunctional lkr/sdh locus of plants also encodes a highly active monofunctional lysine-ketoglutarate reductase using a polyadenylation signal located within an intron. | both plants and animals catabolize lysine (lys) via two consecutive enzymes, lys-ketoglutarate reductase (lkr) and saccharopine dehydrogenase (sdh), which are linked on a single polypeptide encoded by a single lkr/sdh gene. we have previously shown that the arabidopsis lkr/sdh gene also encodes a monofunctional sdh that is transcribed from an internal promoter. in the present report, we have identified two cdnas derived from cotton (gossypium hirsutum) boll abscission zone that encode a novel en ... | 2002 | 12226495 |
| plant allocation to defensive compounds: interactions between elevated co(2) and nitrogen in transgenic cotton plants. | plant allocation to defensive compounds in response to growth in elevated atmospheric co(2) in combination with two levels of nitrogen was examined. the aim was to discover if allocation patterns of transgenic plants containing genes for defensive chemicals which had not evolved in the species would respond as predicted by the carbon nutrient balance (cnb) hypothesis. cotton plants (gossypium hirsutum l.) were sown inside 12 environmental chambers. six of them were maintained at an elevated co(2 ... | 2002 | 11807136 |
| [cloning and characterization of d-113 gene promoter from cotton]. | to study the expression of late embryogenesis abundant gene in seeds, the 1,024 bp 5' flanking sequence of d-113 gene, a late embryogenesis abundant gene of gossypium hirsutum cv. coker 312, was cloned by pcr. the similarity compared with the sequence of lea protein gene family published was 92.50%. there are three putative abres and one enhancer-like which riches a/t in the promoter. the promoter was fused to the beta-glucuronidase gene to form pld ii. via a particle bombardment, pld ii was int ... | 2002 | 11902000 |
| biochemical and molecular inhibition of plastidial carbonic anhydrase reduces the incorporation of acetate into lipids in cotton embryos and tobacco cell suspensions and leaves. | two cdnas encoding functional carbonic anhydrase (ca) enzymes were recently isolated from a non-photosynthetic, cotyledon library of cotton (gossypium hirsutum) seedlings with putative plastid-targeting sequences (genbank accession nos. af132854 and af132855). relative ca transcript abundance and enzyme activity increased 9 and 15 times, respectively, in cotton embryos during the maximum period of reserve oil accumulation. specific sulfonamide inhibitors of ca activity significantly reduced the ... | 2002 | 11950990 |
| paget's disease of bone in lancashire and arsenic pesticide in cotton mill wastewater: a speculative hypothesis. | a 1974 survey of paget's disease of bone in 31 uk towns identified a cluster of six lancashire former cotton mill towns with the highest prevalence rates of the disease and noted a link with the cotton industry. the two highest-prevalence towns are situated on estuaries and are jointly the smallest of the six by the size of their former cotton industry. the two lowest-prevalence towns of the six are highest above sea level and jointly the largest by the size of their former cotton industry. this ... | 2002 | 12231419 |
| efficacy of the organic-certified insecticide diatect ii against the boll weevil (anthonomus grandis) in cotton. | the efficacy of the organic insecticide diatect ii against boll weevil (anthonomus grandis boheman) in cotton (gossypium hirsutum l) in the lower rio grande valley of texas were assessed in small-plot field trials and greenhouse cage tests using azinphos-methyl treatments as a standard for comparison. plastic sheets were placed in the furrows of the treated plots to retrieve boll weevils which dropped from the plants after being killed by the insecticides. samples of live weevils taken by a trac ... | 2002 | 12400443 |
| four near-isogenic lines of cotton with different genes for bacterial blight resistance. | abstract the development and genetic characterization of four near-isogenic lines (nils) of cotton (gossypium hirsutum) is described herein. each line contains a single, but different, gene for resistance to bacterial blight caused by xanthomonas campestris pv. malvacearum. the lines were derived using at least six backcrosses to the susceptible recurrent parent 'acala 44', followed by single plant-progeny row selection for uniformity. the nils are homozygous for the b(2), b(4), b(in), or b(7) g ... | 2002 | 18943887 |
| rotylenchulus reniformis management in cotton with crop rotation. | one-year crop rotations with corn or highly resistant soybean were evaluated at four locations for their effect on rotylenchulus reniformis population levels and yield of a subsequent cotton crop. four nematicide (aldicarb) regimes were included at two of the locations, and rotation with reniform-susceptible soybean was included at the other two locations. one-year rotations to corn or resistant soybean resulted in lower r. reniformis population levels (p </= 0.05) than those found in cotton at ... | 2003 | 19265975 |
| specific expression of a beta-tubulin gene (ghtub1) in developing cotton fibers. | a cdna library was constructed using poly (a)(+) rna isolated from -1-15 dpa fibers of upland cotton (gossypium hirsutum). the cdna encoding a beta-tubulin isoform (designated as ghtub1) was identified through est search. northern blot analysis using 3'-utr of the cdna as a gene-specific probe was performed to investigate the expression levels of ghtub1 in various organs and in the developing fibers. the results showed that ghtub1 gene was specifically expressed in cotton fiber cells. during fib ... | 2003 | 18763138 |
| competition of meloidogyne incognita and rotylenchulus reniformis on cotton following separate and concomitant inoculations. | it has been hypothesized rotylenchulus reniformis (rr) has a competitive advantage over meloidogyne incognita (mi) in the southeastern cotton production region of the united states. this study examines the reproduction and development of meloidogyne incognita (mi) and rotylenchulus reniformis (rr) in separate and concomitant infections on cotton. under greenhouse conditions, cotton seedlings were inoculated simultaneously with juveniles (j2) of m. incognita and vermiform adults of r. reniformis ... | 2003 | 19262774 |
| impact of cotton production systems on management of hoplolaimus columbus. | the effectiveness of selected cultural practices in managing the columbia lance nematode, hoplolaimus columbus, on cotton was evaluated in experiments in growers' infested fields. the effects of planting date, cotton cultivar, treatment with the growth regulator mepiquat chloride, and destruction of cotton-root systems after harvest on cotton-lint yield and population densities of h. columbus were studied. the yield of cotton cultivar deltapine 50 was negatively related (p = 0.054) to initial po ... | 2003 | 19265977 |
| recombinantly expressed isoenzymic aminopeptidases from helicoverpa armigera (american cotton bollworm) midgut display differential interaction with closely related bacillus thuringiensis insecticidal proteins. | several investigators have independently identified membrane-associated aminopeptidases in the midgut of insect larvae as the initial interacting ligand to the insecticidal crystal proteins of bacillus thuringiensis. though several isoenzymes of aminopeptidases have been identified from the midgut of an insect and their corresponding cdna cloned, only one of the isoform has been expressed heterologously and studied for its binding to cry toxins. here we report the cloning and expression of two a ... | 2003 | 12441000 |
| a host-specific bacteria-to-plant signal molecule (nod factor) enhances germination and early growth of diverse crop plants. | lipo-chitooligosaccharides (lcos), or nod factors, are host-specific bacteria-to-plant signal molecules essential for the establishment of a successful n(2)-fixing legume-rhizobia symbiosis. at submicromolar concentrations nod factors induce physiological changes in host and non-host plants. here we show that the nod factor nod bj v(c18:1,mefuc) of bradyrhizobium japonicum 532c enhances germination of a variety of economically important plants belonging to diverse botanical families: zea mays, o ... | 2003 | 12520335 |
| evaluation of efficacy and safety of a herbal medicine used for the treatment of malaria. | resistance of plasmodium falciparum to chloroquine has been reported in several countries. other anti-malarial drugs in use are expensive and not readily accessible to most people in malaria endemic countries. this has led to renewed interest in the development of herbal medicines that have the potential to treat malaria with little or no side effects. this study obtained a preliminary information on the safety and effectiveness of a plant decoction (am-1), used in treating malaria. the am-1 is ... | 2003 | 12820245 |
| slow desiccation leads to high-frequency shoot recovery from transformed somatic embryos of cotton (gossypium hirsutum l. cv. coker 310 fr). | in agrobacterium-mediated genetic transformation of cotton (gossypium hirsutum l. cv. coker 310fr) the frequency at which somatic embryos were converted to plantlets was significantly improved by subjecting the embryos to slow physical desiccation. we used agrobacterium strain gv3101 containing the binary vector pgsfr with the nos-nptii gene for in vitro selection and the 35s gus-int fragment as a reporter to optimize the transformation protocol. although the concentration of kanamycin was reduc ... | 2003 | 12835904 |
| united states department of agriculture-agricultural research service studies on polyketide toxins of fusarium oxysporum f sp vasinfectum: potential targets for disease control. | a group of 133 isolates of the cotton wilt pathogen fusarium oxysporum schlecht f sp vasinfectum (atk) sny & hans, representing five races and 20 vegetative compatibility groups within race 1 were used to determine the identity, biosynthetic regulation and taxonomic distribution of polyketide toxins produced by this pathogen. all isolates of f oxysporum f sp vasinfectum produced and secreted the nonaketide naphthazarin quinones, bikaverin and norbikaverin. most isolates of race 1 (previously den ... | 2003 | 12846324 |
| field and laboratory evaluations of transgenic cottons expressing one or two bacillus thuringiensis var. kurstaki berliner proteins for management of noctuid (lepidoptera) pests. | field studies were conducted from 1999 to 2001 to evaluate the efficacy of the transgenic cotton, gossypium hirsutum (l.), genotype, bollgard ii (monsanto 15985), which expresses two bacillus thuringiensis berliner (bt) proteins (cry1ac + cry2ab) that are active against lepidopterous pests. bollgard ii was compared with bollgard (dp50b), which expresses only one bt protein (cry1ac), and, in all tests, the conventional variety, dp50, was used as a non-bt control. larval populations of the bollwor ... | 2003 | 12852613 |
| 8-hydroxy-(+)-delta-cadinene is a precursor to hemigossypol in gossypium hirsutum. | [(3)h](+)-delta-cadinene and its 8-hydroxy derivative, prepared from (1rs)-[1-(3)h]fpp by the action of one and two recombinant enzymes, respectively, were infiltrated into cotyledons of bacterial blight-resistant cotton plants as they biosynthesized sesquiterpene phytoalexins in response to infection by xanthomonas campestris pv. malvacearum. following both treatments, tritium appeared in the hplc fraction that contained hemigossypol. hemigossypol was isolated from the cotyledons that had been ... | 2003 | 12946420 |
| efficacy of transgenic cotton containing a cry1ac gene from bacillus thuringiensis against helicoverpa armigera (lepidoptera: noctuidae) in northern china. | nucotn 33b, a bt transgenic variety of upland cotton (gossypium hirsutum l.) expressing the insecticidal protein cry1ac from bacillus thuringiensis berliner sp. kurstaki, was evaluated for resistance to helicoverpa armigera (hübner) during 1998-2000 in northern china. the results indicated that there was no significant difference in egg densities between nucotn 33b and three nontransgenic varieties (dp5415, zhongmian12, and shiyuan321) during the season, although the survival of larvae on nucotn ... | 2003 | 14503607 |
| [cloning and characterization of the pts2 receptor gene (ghpex7) from cotton (gossypium hirsutum l.)]. | peroxisomal targeting signals (pts), including pts1 and pts2, were proposed to play an important role in introducing proteins into the peroxisomal matrix. pex7, the pts2 receptor, is crucial to the import of pts2 proteins. based on the sequence of a fragment (f010) recovered from cdna-aflp, the full-length cdna sequence was obtained by race and the contig in cotton ests, and the coding sequence was further cloned. the ghpex7 cdna, 1314 bp in length, contained 5 non-coding (77 bp) upstream, 3 com ... | 2003 | 14577373 |
| overwintering of heliothis virescens (f.) and helicoverpa zea (boddie) (lepidoptera: noctuidae) in cotton fields of northeast mississippi. | following the 1995-2001 crop seasons, population densities of pupae of tobacco budworm, heliothis virescens (f.), and corn earworm, helicoverpa zea (boddie), overwintering in cotton fields, gossypium hirsutum l., in monroe county, ms, were estimated by digging a total of 43,542 m-row. densities of pupae varied greatly among years with the highest densities occurring in years with low rainfall in the late summer and early fall. the sex ratio of pupae was variable and significantly male-biased in ... | 2003 | 14650516 |
| early-season colonization patterns of the boll weevil (coleoptera: curculionidae) in central texas cotton. | it is commonly believed that colonization of early-season cotton, gossypium hirsutum l., by overwintered boll weevils, anthonomus grandis grandis boheman, is concentrated on field margins. however, supporting experimental evidence is not available. in 1999 and 2000, we examined colonization patterns of overwintered boll weevils in central texas cotton on the bases of adult collections by a pneumatic sampler and hand collections of abscised infested squares. samples were taken from sites arranged ... | 2003 | 14994797 |
| molecular cloning, differential expression, and functional characterization of a family of class i ubiquitin-conjugating enzyme (e2) genes in cotton (gossypium). | two cdnas and their corresponding genes (ghubc1 and ghubc2) encoding ubiquitin-conjugating enzymes (e2s) have been cloned and characterized from allotetraploid cotton gossypium hirsutum ((ad)(1) genome). three additional e2 genes (gaubc1, gtubc2, and grubc2) have also been identified from diploid cottons gossypium arboreum (a(2) genome), gossypium thurberi (d(1) genome), and gossypium raimondii (d(5) genome), respectively. the derived amino acid sequences of the five closely related cotton e2s a ... | 2003 | 12591614 |
| two isoforms of rubisco activase in cotton, the products of separate genes not alternative splicing. | in several plant species, ribulose-1,5-bisphosphate carboxylase/oxygenase (rubisco) activase consists of two isoforms that are produced by alternative splicing of a pre-mrna. two forms of activase corresponding to the longer, redox-regulated alpha and the shorter, beta forms were detected immunologically in cotton (gossypium hirsutum l.) leaves, but their n-termini differed in 4 of 14 residues. the cdnas for the alpha and beta forms of cotton activase diverged throughout the translated and 3'-un ... | 2003 | 12624760 |
| effects of black-eyed pea trypsin/chymotrypsin inhibitor on proteolytic activity and on development of anthonomus grandis. | the cotton boll weevil anthonomus grandis (boheman) is one of the major pests of cotton (gossypium hirsutum l.) in tropical and sub-tropical areas of the new world. this feeds on cotton floral fruits and buds causing severe crop losses. digestion in the boll weevil is facilitated by high levels of serine proteinases, which are responsible for the almost all proteolytic activity. aiming to reduce the proteolytic activity, the inhibitory effects of black-eyed pea trypsin/chymotrypsin inhibitor (bt ... | 2003 | 12737983 |
| characterization of the targeting signal of the arabidopsis 22-kd integral peroxisomal membrane protein. | using a combination of in vivo and in vitro assays, we characterized the sorting pathway and molecular targeting signal for the arabidopsis 22-kd peroxisome membrane protein (pmp22), an integral component of the membrane of all peroxisomes in the mature plant. we show that nascent pmp22 is sorted directly from the cytosol to peroxisomes and that it is inserted into the peroxisomal boundary membrane with its n- and c-termini facing the cytosol. this direct sorting of pmp22 to peroxisomes contrast ... | 2003 | 12972647 |
| an atp-binding cassette transporter ghwbc1 from elongating cotton fibers. | we have isolated a cdna (ghwbc1) from cotton (gossypium hirsutum) that encodes an atp-binding cassette transporter of the wbc (white/brown complex) subfamily. members of this subfamily are half-sized transporters and are reported to mediate lipid and drug excretion in human (homo sapiens). ghwbc1 is highly expressed in developing fiber cells, but transcripts were also detectable in other tissues except roots. the transcript level peaked in rapidly expanding fibers from 5 to 9 dpa and then decrea ... | 2003 | 12972649 |
| relationships between tolerance and resistance to meloidogyne incognita in cotton. | the southern root-knot nematode, meloidogyne incognita, is the most damaging pathogen of cotton in the united states, and both resistance and tolerance to m. incognita could be valuable management approaches. our objectives were to evaluate advanced cotton breeding lines for resistance and tolerance to m. incognita and to determine if a relationship between resistance and tolerance exists. reproduction of m. incognita was evaluated on 17 breeding lines, a susceptible control (delta and pine land ... | 2003 | 19262772 |
| physiological and antioxidant responses of cotton and spurred anoda under interference and mild drought. | the influence of plant interference and a mild drought on gas exchange and oxidative stress was investigated using potted plants of two cotton species (gossypium hirsutum l. cv. delta pine 5415, and gossypium barbadense l. cv. pima s-7) and spurred anoda (anoda cristata l. schlecht.) of the malvaceae. without interference, cotton and spurred anoda had similar net photosynthesis (pnet) but different pigment profiles. stomatal conductance (gs) and transpiration rate (e) were greater in spurred ano ... | 2003 | 14504299 |
| localization of qtls for yield and fiber quality traits of tetraploid cotton cultivar. | using ssr and rapd as molecular markers, and the 69 f2 families from a cross between handan208 (gossypium hirsutum) and pima90 (gossypium barbadense) as a mapping population, a linkage map comprising 126 markers was constructed. with an average spacing 13.7 cm between markers, the linkage map spanned 1,717.0 cm, which covers approximately 34.34% of the total recombinational length of cotton genome. based on the linkage map and the f2:3 phenotypic data, overall genome qtl screening was conducted ... | 2003 | 12924159 |
| a combined rflp-ssr-aflp map of tetraploid cotton based on a gossypium hirsutum x gossypium barbadense backcross population. | an interspecific gossypium hirsutum x gossypium barbadense backcross population of 75 bc1 plants was evaluated for 1014 markers. the map consists of 888 loci, including 465 aflps, 229 ssrs, 192 rflps, and 2 morphological markers, ordered in 37 linkage groups that represent most if not all of the 26 chromosomes, altogether spanning 4400 cm. loci were not evenly distributed over linkage groups, and 18 of the 26 long groups had a single dense region. this paper proposes a partially revised list of ... | 2003 | 12897870 |
| the dna sequence of a gypsy element from gossypium hirsutum l. and characterization of gypsy elements in three gossypium species. | a strategy was developed to isolate a complete gypsy-element from gossypium hirsutum l. based on the sequence of a rapd that was polymorphic in near isoganic lines of cotton that varied in leaf shape. a 5998 nt clone was isolated and its gene order and sequence confirmed it was a gypsy-type retroelement. sequences homologous to the gag portion of this clone were also found in gossypium herbaceum l. and gossypium raimondii l. a portion of the open reading frame of the integrase gene was amplified ... | 2003 | 14631654 |
| effects of drought stressed cotton, gossypium hirsutum l., on beet armyworm, spodoptera exigua (hübner), oviposition, and larval feeding preferences and growth. | the beet armyworm, spodoptera exigua (hübner), has been anecdotally reported to oviposit more on drought stressed than on nonstressed cotton plants. using potted cotton plants in cages, this study demonstrated that beet armyworms deposited 3.3, 4.6, and 2.3 times more (p < or = 0.05) eggs on cotton plants that were grown on 1500, 1000, and 750 ml water/wk, respectively, than on cotton plants grown in well watered (4000 ml water/wk) soil. third instars, however, showed no preference for stressed ... | 2003 | 14584672 |
| identification of ghmyb109 encoding a r2r3 myb transcription factor that expressed specifically in fiber initials and elongating fibers of cotton (gossypium hirsutum l.). | cotton (gossypium hirsutum l.) fibers are derived from ovule epidermis, which are developmentally similar to arabidopsis trichome where several myb transcription factors have been shown to control their formation. however, little is known about the molecular control of cotton fiber initiation. here we isolated 55 cotton myb domain-containing sequences expressed in ovules during fiber initiation. among them, ghmyb109, encoding a r2r3 myb transcription factor of 234 amino acids, was found to be st ... | 2003 | 14580676 |
| transgenic overproduction of glutathione reductase does not protect cotton, gossypium hirsutum (malvaceae), from photoinhibition during growth under chilling conditions. | in some studies, tissues from plants that have been genetically transformed to overproduce antioxidant enzymes sustain less damage when abruptly exposed to short-term chilling in the laboratory. however, few studies have examined the performance of transgenic plants during longer-term growth under chilling conditions. we compared growth of transgenic cotton that overproduces glutathione reductase (gr+; ∼40-fold overproduction) to growth of the wild type in a controlled environment chamber as lea ... | 2003 | 21659239 |
| [evaluation of the effect of pollen irradiation on karyotype variability in cotton plants]. | the effect of pollen irradiation at dose rates of 10, 15, 20, and 25 gy on variability in cotton plants gossypium hirsutum l. was studied. the modified plants showed a reduced fertility, mainly caused by chromosomal rearragements and genomic mutations during meiosis. the genomic mutations involved primary and tertiary monosomics, monotelodisomics, and a haploid plant. the decrease in meiotic index and pollen fertility in the cotton aneuploids was related not only to aberrations in chromosome pai ... | 2003 | 12942779 |
| variation in surface chemical constituents of cotton (gossypium hirsutum) fiber as a function of maturity. | modern cotton yarn production technology has made it imperative that new predictors of yarn spinning efficiency be determined. surface frictional forces play a large role in spinning efficiency, yet little is known about the chemical constituents comprising the cotton fiber surface or their respective roles in inter-fiber frictional behavior. major cotton fiber surface chemical components including pectin, wax, soluble salts, and sugars were quantified, and their respective relationships to cott ... | 2003 | 14690385 |
| changes in oxidative stress enzymes during artificial ageing in cotton (gossypium hirsutum l.) seeds. | the present study was carried out to elucidate the mechanism of seed deterioration in two cotton (gossypium hirsutum l.) cultivars (hs6 and h1098). the seeds were artificially aged at 40 +/- 1 degree c and 100% relative humidity for 4 days. in both cultivars, germinability decreased, whereas membrane deterioration, as assayed by electrical conductivity of the seed leachates, increased progressively with artificial ageing. the decrease in germinability was well correlated with increased accumulat ... | 2003 | 14593811 |
| [evaluation of the effect of pollen irradiation on karyotype variability in m2 cotton plants]. | the karyotypes of biomorphologically abnormal cotton (gossypium hirsutum l.) plants obtained in m2 after pollination with pollen irradiated at dose rates 10, 15, 20, and 25 gy were studied. various genomic and chromosomal mutations were detected in 57 m2 families. the primary monosomics isolated in m2 were found to be cytologically more stable and more viable, since they had higher meiotic indices, pollen fertility, and seed formation. in m2, a decrease in the number of plants with multiple kary ... | 2003 | 14515465 |
| field evaluation of a helicoverpa zea (lepidoptera: noctuidae) damage simulation model: effects of irrigation, h. zea density, and time of damage on cotton yield. | helicoverpa zea (boddie) is an important pest of cotton, gossypium hirsutum l., for which many economic injury and population models have been developed to predict the impact of injury by this species on cotton yield. a number of these models were developed using results from simulated damage experiments, despite the fact that no studies have demonstrated that simulated damage is comparable to real h. zea damage. our main objective in this study was to compare the effect on yield of h. zea larva ... | 2003 | 14503589 |
| [genetic analysis of the duration of the vegetative period in cotton gossypium hirsutum l. with background of various nitrogen level in soil]. | using hayman's model of genetic analysis of the f1 hybrids from crosses between early- and late-ripening forms of cotton gossypium hirsutum l., we examined inheritance of the duration of vegetative period at various contents of nitrogen in soil. two variants of hybrid plants were studied. with the natural content of soil nitrogen, incomplete directionally dominance of earliness over late ripening with prevalence of additive gene effect of nonallelic interactions was observed. the genetic formula ... | 2003 | 14964832 |
| estimate of alabama argillacea (hübner) (lepidoptera: noctuidae) development with nonlinear models. | the objective of this work was to evaluate which nonlinear model [davidson (1942, 1944), stinner et al. (1974), sharpe & demichele (1977), and lactin et al. (1995)] best describes the relationship between developmental rates of the different instars and stages of alabama argillacea (hübner) (lepidoptera: noctuidae), and temperature. a. argillacea larvae were fed with cotton leaves (gossypium hirsutum l., race latifolium hutch., cultivar cnpa 7h) at constant temperatures of 20, 23, 25, 28, 30, 33 ... | 2003 | 15029370 |
| recovery from terminal and fruit damage by dry season cotton crops in tropical australia. | we conducted a 2-yr field study on growth and yield compensation in cotton (gossypium hirsutum l.) after artificial pest damage in kununurra in nw australia. the aim was to assess the responses of cotton grown in tropical australia to simulated helicoverpa damage early in the season (tip damage) and during fruiting (square removal). in this region, cotton is grown in an inverse temperature regimen to cotton grown in temperate regions, and it is possible that tropical cotton crops exhibit differe ... | 2003 | 12852610 |
| viewing the difference between the diploid and the polyploid in the light of the upland cotton aneuploid. | the aneuploidy of gossypium hirsutum l. (upland cotton) aneusomatics were obtained by induced parthenogenesis. these aneuploids could grow and set seeds normally. in the process of meiosis there appeared large quantities of heteromorphic pairs and multivalent chromosomes and many cases of cytomixis and multisperm fertilization occurred. the aneuploids produced offsprings through sexual propagation. we explored penetratingly the questions how and why these aneuploids could survive. through this r ... | 2003 | 12830987 |
| effects of ultraviolet-b radiation on cotton (gossypium hirsutum l.) morphology and anatomy. | cotton (gossypium hirsutum l.) crop, cultivated between 40 degrees n and 40 degrees s, is currently experiencing 2-11 kj m-2 d-1 of uv-b radiation. this is predicted to increase in the near future. an experiment was conducted to study the effect of enhanced uv-b radiation on vegetative and reproductive morphology and leaf anatomy of cotton in sunlit, controlled environment chambers. from emergence to harvest, cotton plants were exposed to 0, 8 or 16 kj m-2 d-1 of uv-b in a square wave approach f ... | 2003 | 12770842 |
| effect of urea fertilizer application on soluble protein and free amino acid content of cotton petioles in relation to silverleaf whitefly (bemisia argentifolii) populations. | the impact of urea nitrogen fertilization on silverleaf whitefly, bemisia argentifolii bellows & perring, population dynamics was examined in field-grown cotton (gossypium hirsutum l.). five urea nitrogen treatments were tested, consisting of soil applications of 0, 112, 168, and 224 kg nitrogen per hectare, and acombined soil-foliar application of 112:17 kg nitrogen per hectare. a positive response was observed between n application rates and the measured levels of nitrate n in petioles from ma ... | 2003 | 12757331 |
| localization of sucrose synthase and callose in freeze-substituted secondary-wall-stage cotton fibers. | methods for cryogenic fixation, freeze substitution, and embedding were developed to preserve the cellular structure and protein localization of secondary-wall-stage cotton (gossypium hirsutum l.) fibers accurately for the first time. perturbation by specimen handling was minimized by freezing fibers still attached to a seed fragment within 2 min after removal of seeds from a boll still attached to the plant. these methods revealed native ultrastructure, including numerous active golgi bodies, m ... | 2003 | 12802624 |
| high-frequency somatic embryo production and maturation into normal plants in cotton (gossypium hirsutum) through metabolic stress. | a highly efficient somatic embryo production and maturation procedure has been developed to regenerate plantlets from cotton ( gossypium hirsutum). this procedure involves the acceleration of differentiation through manipulations of nutrient and microenvironment conditions. embryogenic calli, initiated from hypocotyls or cotyledonary leaf sections on ms medium containing 0.1 mg/l 2,4 dichlorophenoxyacetic acid, 0.5 mg/l kinetin, and 3% maltose produced globular-stage somatic embryos when transfe ... | 2003 | 12789412 |
| the crystal structure of annexin gh1 from gossypium hirsutum reveals an unusual s3 cluster. | the three-dimensional crystal structure of recombinant annexin gh1 from gossypium hirsutum (cotton fibre) has been determined and refined to the final r-factor of 0.219 at the resolution of 2.1 a. this plant annexin consists of the typical 'annexin fold' and is similar to the previously solved bell pepper annexin anx24(ca32), but significant differences are seen when compared to the structure of nonplant annexins. a comparison with the structure of the mammalian annexin anxa5 indicates that cano ... | 2003 | 12787021 |
| genes duplicated by polyploidy show unequal contributions to the transcriptome and organ-specific reciprocal silencing. | most eukaryotes have genomes that exhibit high levels of gene redundancy, much of which seems to have arisen from one or more cycles of genome doubling. polyploidy has been particularly prominent during flowering plant evolution, yielding duplicated genes (homoeologs) whose expression may be retained or lost either as an immediate consequence of polyploidization or on an evolutionary timescale. expression of 40 homoeologous gene pairs was assayed by cdna-single-stranded conformation polymorphism ... | 2003 | 12665616 |
| subgenome-specific markers in allopolyploid cotton gossypium hirsutum: implications for evolutionary analysis of polyploids. | we developed a set of genetic markers specific to the a and d genome types of cotton using representational difference analysis (rda). these markers produce amplification products with genomic dna from allotetraploid cotton gossypium hirsutum. one of the markers is a polymorphic amplified restriction fragment (parf) - a sequence found in both a and d genomes but differently flanked by restriction sites. results of phylogenetic analysis of the parf sequences from diploid cottons and from allotetr ... | 2003 | 12657471 |
| inheritance and fine mapping of fertility restoration for cytoplasmic male sterility in gossypium hirsutum l. | genetics of cms fertility restoration was presented through the analysis of classic genetics and molecular markers. based on f(2) segregation of the crosses between cms and the restoring lines, the testcrosses and f(1) x f(1) populations, together with rapd and ssr mapping, one dominant gene was identified to control the cms fertility restoration in cotton. the strategy of genotype representation analysis (gra) was put forward to screen the markers linked with the rf(1) locus. using 1,025 random ... | 2003 | 12589546 |
| the cotton kinesin-like calmodulin-binding protein associates with cortical microtubules in cotton fibers. | microtubules in interphase plant cells form a cortical array, which is critical for plant cell morphogenesis. genetic studies imply that the minus end-directed microtubule motor kinesin-like calmodulin-binding protein (kcbp) plays a role in trichome morphogenesis in arabidopsis. however, it was not clear whether this motor interacted with interphase microtubules. in cotton (gossypium hirsutum) fibers, cortical microtubules undergo dramatic reorganization during fiber development. in this study, ... | 2003 | 12746521 |
| quantitative analysis of transcript accumulation from genes duplicated by polyploidy using cdna-sscp. | repeated rounds of polyploidy have been commonplace in the lineages leading to modern eukaryotic genomes, giving rise to widespread gene duplication. genes duplicated by polyploidy, or "homoeologs," may continue to be expressed at equal levels following polyploidization or their expression may be dramatically altered. in this report, we describe how sscp analysis of rt-pcr products can be used to evaluate the expression status (presence and relative quantity) of highly similar homoelogous gene p ... | 2003 | 12703297 |
| identification of a third fuzzless seed locus in upland cotton (gossypium hirsutum l.). | segregating populations were developed to evaluate the inheritance of the fuzzless seed phenotypes in upland cotton (gossypium hirsutum l.). accession 143 of the mississippi obsolete variety collection (movc) has a fuzzless seed phenotype. this line carries the n(2) locus which is recessive to the seed fuzz phenotype. data from the f(2), bc(1)f(1), f(2:3), and bc(1)f(2) populations of dp 5690 x 143 fit a two-loci model for expression of the recessive fuzzless seed phenotype. fuzzless seeds were ... | 2003 | 12547925 |
| the use of multiple alien chromosome addition aneuploids facilitates genetic linkage mapping of the gossypium g genome. | primary germplasm pools represent the most accessible source of new alleles for crop improvement, but not all effective alleles are available in the primary germplasm pool, and breeders must sometimes confront the difficulties of introgressing genes from the secondary and tertiary germplasm pools in cotton by using synthetic polyploids as introgression bridges. two parental gossypium nelsonii x gossypium australe aflp genetic linkage maps were used to identify g genome chromosome-specific molecu ... | 2003 | 14608394 |
| effects of nematicides on cotton root mycobiota. | baseline information on the diversity and population densities of fungi collected from soil debris and cotton (gossypium hirsutum l.) roots was determined. samples were collected from tifton, ga, and starkville, ms containing cotton field soil treated with the nematicides 1,3-dichloroproprene (fumigant) and aldicarb (granules). a total of 10,550 and 13,450 fungal isolates were collected from these two study sites, respectively. of this total, 34 genera of plant pathogenic or saprophytic species ... | 2004 | 15119856 |
| cotton fiber germin-like protein. i. molecular cloning and gene expression. | the presence of cotton ( gossypium hirsutum l.) fiber transcripts coding for a germin-like protein (glp) was revealed by differential display analysis in which early stages of cotton fiber development between a wild type line, texas marker-1 (tm1) and a near isogenic mutant, naked seed (n1) were compared. transcripts of the cotton glp ( ghglp1) accumulated specifically in tm1, but did not accumulate in the mutant although the ghglp1 gene was present in both lines. the deduced protein sequence of ... | 2004 | 14648117 |
| a plant-specific kinesin binds to actin microfilaments and interacts with cortical microtubules in cotton fibers. | a novel kinesin, ghkch1, has been identified from cotton (gossypium hirsutum) fibers. ghkch1 has a centrally located kinesin catalytic core, a signature neck peptide of minus end-directed kinesins, and a unique calponin homology (ch) domain at its n terminus. ghkch1 and other ch domain-containing kinesins (kchs) belong to a distinct branch of the minus end-directed kinesin subfamily. to date the kch kinesins have been found only in higher plants. because the ch domain is often found in actin-bin ... | 2004 | 15557092 |
| cotton-fiber germin-like protein. ii: immunolocalization, purification, and functional analysis. | cotton (gossypium hirsutum l.) contains a germin-like protein (glp), ghglp1, that shows tissue-specific accumulation in fiber. the fiber glp is an oligomeric, glycosylated protein with a subunit size of approximately 25.5 kda. accumulation of ghglp1 occurs during the period of fiber elongation [4-14 days post-anthesis (dpa)]. during early phases of fiber development (2-4 dpa), ghglp1 localizes to cytoplasmic vesicles as shown by confocal immunofluorescent microscopy. in slightly older fibers (7- ... | 2004 | 14634817 |
| volatiles released from cotton plants in response to helicoverpa zea feeding damage on cotton flower buds. | feeding of helicoverpa zea larvae on cotton ( gossypium hirsutum l.) flower buds (squares) for 24 or 48 h induced the release of a number of terpenes [( e)-beta-ocimene, linalool, ( e)-beta-farnesene, ( e, e)-alpha-farnesene, ( e)-4,8-dimethyl-1,3,7-nonatriene, ( e, e)-4,8,12-trimethyl-1,3,7,11-tridecatetraene], isomeric hexenyl butyrates, 2-methylbutyrates, indole and ( z)-3-hexenyl acetate. these compounds are not released in significant amounts from undamaged squares and freshly damaged squar ... | 2004 | 14625774 |
| cloning and expression analysis of a bzip cdna in gossypium hirsutum l. | a new full-length cdna clone was isolated from the fiber cdna library of gossypium hirsutum l. the cdna, designated ghbzip,encoded a polypeptide of 645 amino acids. ghbzip protein had the structure characteristics of plant bzip proteins, including two conserved domains, duf630 and duf632 with unknown functions, a proline-rich domain and a phenylalanine-rich domain. meanwhile, the protein contained a leucine zipper-like motif in duf632. the hydropathy analysis showed that the ghbzip was a membran ... | 2004 | 15490881 |
| [the structural characteristics, alternative splicing and genetic experession analysis of adp-ribosylation-factor 1 (arf1) in cotton]. | the full-length cdna,dna and promoter of adp-ribosylation-factor 1 (arf1) was isolated from gossypium hirsutum y18 by means of isocaudarner inverse pcr (ii-pcr) and rapid isolating cdna 5' unknown sequence and promoter (ricup) established in our lab. results indicated that the gene is 4 360 bp in size, including seven exons and six introns. interestingly, alterative splicing occurs at intron i. differential processing of intron 1 yields three different transcripts with 1 026 bp, 1103 bp and 1 54 ... | 2004 | 15481542 |
| cloning, characterization, and evolution of the nbs-lrr-encoding resistance gene analogue family in polyploid cotton (gossypium hirsutum l.). | the nucleotide-binding site-leucine-rich repeat (nbs-lrr)-encoding gene family has attracted much research interest because approximately 75% of the plant disease resistance genes that have been cloned to date are from this gene family. we cloned the nbs-lrr-encoding genes from polyploid cotton by a polymerase chain reaction-based approach. a sample of 150 clones was selected from the nbs-lrr gene sequence library and was sequenced, and 61 resistance gene analogs (rga) were identified. sequence ... | 2004 | 15553248 |
| recovery of leaf area through accelerated shoot ontogeny in thrips-damaged cotton seedlings. | leaf area of cotton seedlings (gossypium hirsutum) can be reduced by as much as 50 % by early season thrips infestations, but it is well documented that plants can regain the difference in leaf area once infestation ceases. the processes involved in the recovery have not been identified. hypotheses include enhancement of the photosynthetic rate of the damaged leaves, more efficient leaf construction (i.e. more leaf area per unit of dry matter invested in new leaves), and more branching. | 2004 | 15155380 |
| characterization of the brassinosteroid insensitive 1 genes of cotton. | suppression of brassinosteroid (br) biosynthesis in cotton ovules by treatment with brassinazole inhibits fiber formation, indicating that br plays an important role in cotton fiber development. plant responses to brassinosteroids (br) are mediated through a plasma membrane-bound leucine-rich repeat (lrr) receptor-like protein kinase known as bri1. mutations in the bri1 genes of several species result in dwarfed plants with reduced sensitivity to br. a single expressed sequence tag (est) from co ... | 2004 | 15159624 |
| [cloning and characterization of two beta-mannosidase cdnas in gossypium hirsutum l]. | by using the method of pcr-based cdna library screening, two beta-mannosidase clones, ghmana1 and ghmana2, had been isolated. ghmana1 had a length of 2692 bp coding for a polypeptide of 834 amino acids, and ghmana2 was 3209 bp which encoded a polypeptide of 976 amino acids. ghmana1 and ghmana2 shared an identical sequence of 747 amino acids in their carboxyl-terminals, but were distinctly different in their amino-terminals. both beta-mannosidases were members of glycosyl hydrolase family 2, whic ... | 2004 | 15599050 |
| terpenoid aldehyde formation and lysigenous gland storage sites in cotton: variant with mature glands but suppressed levels of terpenoid aldehydes. | a new cotton variant with reduced levels of terpenoid aldehydes (sesquiterpenoids and sesterterpenoids (heliocides)) was isolated from the progeny of hemizygous cotton (gossypium hirsutum cv. coker 312) transformed with antisense (+)-delta-cadinene synthase cdna. southern analysis of leaf dna digested with hindiii, pst or kpni restriction endonucleases did not detect any antisense cdn1-c1 dna in the genome of the variant. the gossypol content in the seed of the variant was markedly lower than in ... | 2004 | 15231408 |
| leaf and canopy photosynthetic characteristics of cotton (gossypium hirsutum) under elevated co2 concentration and uv-b radiation. | increases in both atmospheric co2 concentration ([co2]) and ultraviolet-b (uv-b) radiation on the earth's surface are features of current climate change patterns. an experiment was conducted in sunlit, controlled environment chambers known as soil-plant-atmosphere-research (spar) units to determine interactive effects of elevated [co2] and uv-b radiation on leaf and canopy photosynthetic characteristics of cotton. six treatments were comprised of two co2 levels of 360 (ambient) and 720 (elevated ... | 2004 | 15202715 |
| wide coverage of the tetraploid cotton genome using newly developed microsatellite markers. | microsatellite [simple-sequence repeat (ssr)] markers were developed and positioned on the genetic map of tetraploid cotton. three hundred and ninety-two unique microsatellite sequences, all but two containing a (ca/gt) repeat, were isolated, and the deduced primers were used to screen for polymorphism between the gossypium hirsutum and g. barbadense parents of the mapping population analyzed in our laboratory. the observed rate of polymorphism was 56%. the 204 polymorphic ssrs revealed 261 segr ... | 2004 | 14997301 |
| msma resistance studies. | monosodium methanearsonate (msma)-resistant and -susceptible common cocklebur (xanthium strumarium l.) and cotton (gossypium hirsutum l.) were treated with msma. plant parameters analyzed were: glutathione synthetase activity, selected amino acid (arginine, glutamic acid, alanine, citrulline, glutamine, and glutathione) content and arsenic content (msma, total arsenic, and arsonate). no reduction of arsenic from the parent pentavalent form present in msma to the trivalent form was detected. argi ... | 2004 | 15473645 |
| cloning and expression analysis of carboxyl-terminal protease cdna from gossypium hirsutum l. | a full-length cotton cdna,designated ghctp, was isolated from gossypium hirsutum l. by the pcr-based cdna library screening. the cdna encodes a polypeptide of 473-amino acid residues. sequence alignment showed that ghctp was highly homologous to a family of carboxyl-terminal proteases (ctp) from other species. ghctp is a potential transmembrane protease, and comprises a conserved domain numbered duf239 at its carboxyl-terminal and an arginine-rich region at the amino-terminal, respectively. furt ... | 2004 | 15473308 |
| molecular cloning and characterization of ghlecrk, a novel kinase gene with lectin-like domain from gossypium hirsutum. | a novel gene encoding a lectin-like protein kinase was cloned from the upland cotton (gossypium hirsutum) through cdna library screening. this gene (named as ghlecrk; genbank accession number: ay487461) had a total length of 2233bp with an open reading frame of 1926bp, and encoded a predicted polypeptide of 641 amino acids with a molecular weight of 71.16kda. the ghlecrk protein shared 73, 65, 64 and 59% identity with other lectin-like kinase proteins isolated from a. thaliana (at3g53810, at2g37 ... | 2004 | 15354356 |
| an optimized gossypol high-performance liquid chromatography assay and its application in evaluation of different gland genotypes of cotton. | a comparative study on gossypol content of various genetic types of pigment glands of cotton varieties was conducted through an optimized high-performance liquid chromatography (hplc) on a c18 column (4.6 mm x 250 mm, 5 microm particle) with methanol-0.5% acetic acid aqueous solution, 90 : 10 (v/v), as mobile phase, at a flow rate of 0.8 ml/min and uv detection at 254 nm. the method was shown to be highly reproducible, with precision [as relative standard deviation (rsd)] and accuracy [as relati ... | 2004 | 15286405 |
| members of a new group of chitinase-like genes are expressed preferentially in cotton cells with secondary walls. | two homologous cotton (gossypium hirsutum l.) genes, ghctl1 and ghctl2, encode members of a new group of chitinase-like proteins (called the ghctl group) that includes other proteins from two cotton species, arabidopsis, rice, and pea. members of the ghctl group are assigned to family gh19 glycoside hydrolases along with numerous authentic chitinases (http://afmb.cnrs-mrs.fr/cazy/index.html), but the proteins have novel consensus sequences in two regions that are essential for chitinase activity ... | 2004 | 15284492 |
| production and characterization of somatic hybrids between upland cotton (gossypium hirsutum) and wild cotton (g. klotzschianum anderss) via electrofusion. | symmetric somatic hybrid plants between gossypium hirsutum coker 201 and g. klotzschianum were obtained through electrofusion. the fusion products were cultured in km8p medium supplemented with 2.685 microm alpha-naphthaleneacetic acid and 0.465 microm kinetin, and the regenerated plants were morphologically, genetically, and cytologically characterized. nuclear-dna flow cytometric analysis revealed that the plants tested (31 of 40) had a relative dna content close to the total dna contents of t ... | 2004 | 15114473 |
| wide-cross whole-genome radiation hybrid mapping of cotton (gossypium hirsutum l.). | we report the development and characterization of a "wide-cross whole-genome radiation hybrid" (wwrh) panel from cotton (gossypium hirsutum l.). chromosomes were segmented by gamma-irradiation of g. hirsutum (n = 26) pollen, and segmented chromosomes were rescued after in vivo fertilization of g. barbadense egg cells (n = 26). a 5-krad gamma-ray wwrh mapping panel (n = 93) was constructed and genotyped at 102 ssr loci. ssr marker retention frequencies were higher than those for animal systems an ... | 2004 | 15280245 |
| injury to preflowering and flowering cotton by brown stink bug and southern green stink bug. | the impact of brown stink bug, euschistus servus (say), and southern green stink bug, nezara viridula (l.), injury was evaluated on preflowering and flowering cotton, gossypium hirsutum l., plants in no-choice tests. vegetative stage cotton seedlings and reproductive structures, including flower buds (square) and bolls, were infested with adults and/or nymphs of both species. there were no significant differences in height, height to node ratio, square retention, and flower initiation for cotton ... | 2004 | 15279273 |
| evaluation of haemoglobin (erythrogen): for improved somatic embryogenesis and plant regeneration in cotton (gossypium hirsutum l. cv. svpr 2). | somatic embryogenesis in cotton (gossypium hirsutum l.) is accelerated when the plant regeneration medium is supplemented with haemoglobin (erythrogen). in cotton svpr 2 lines, a higher frequency of embryoid formation was observed when the medium contained 400 mg/l haemoglobin. fresh weight of the callus, rate of embryoid induction, number of embryoids formed and the percentage of plant regeneration from somatic embryos were increased. among the two different cultivars tested, mcu 11 showed no r ... | 2004 | 15278417 |
| incongruent patterns of local and global genome size evolution in cotton. | genome sizes in plants vary over several orders of magnitude, reflecting a combination of differentially acting local and global forces such as biases in indel accumulation and transposable element proliferation or removal. to gain insight into the relative role of these and other forces, approximately 105 kb of contiguous sequence surrounding the cellulose synthase gene cesa1 was compared for the two coresident genomes (at and dt) of the allopolyploid cotton species, gossypium hirsutum. these t ... | 2004 | 15256507 |
| combined effects of elevated co2 and herbivore damage on alfalfa and cotton. | we examined herbivore-induced responses of alfalfa (medicago sativa) and cotton (gossypium hirsutum) under different co2 conditions. plants were grown under ambient (350 ppm) or elevated (700 ppm) co2 levels, and were either damaged or undamaged by spodoptera littoralis larvae. at harvest, growth of undamaged (control) plants was determined, and foliar chemical composition of both undamaged and damaged plants was analyzed. cotton grew faster overall and showed a greater increase in growth in res ... | 2004 | 15672673 |
| estimating photosynthetic electron transport via chlorophyll fluorometry without photosystem ii light saturation. | estimates of thylakoid electron transport rates (j(e)) from chlorophyll fluorometry are often used in combination with leaf gas exchange measurements to provide detailed information about photosynthetic activity of leaves in situ. estimating j(e) requires accurate determination of the quantum efficiency of photosystem ii (phi(p)), which in turn requires momentary light saturation of the photosystem ii light harvesting complex to induce the maximum fluorescence signal (f(m)'). in practice, full s ... | 2004 | 16151873 |
| evaluation of transgenic cotton varieties and a glyphosate application on seedling disease incidence. | a study was conducted to determine whether stand densities of transgenic cotton (gossypium hirsutum) varieties, with or without glyphosate, were similar to conventional varieties of the same lineage group in georgia and mississippi. transgenic and conventional cotton varieties were placed into five lineage groups of related varieties and seedling disease was evaluated in three greenhouse tests and a field trial using rhizoctonia solani ag-4. seed vigor was determined by standard germination stud ... | 2004 | 15645175 |
| low salt petroleum produced water reuse: a farming alternative outside the food chain. | oil and gas production gives rise to water production depending on the state of maturation of the field. this means large volumes of water available. today, this water is partly re-injected into the reservoir. totalfinaelf's sustainable contribution to preserve water resources is to propose an alternative utilization: the reuse of produced waters from petroleum activities, outside the food chain. the aim of the first part of this study is to demonstrate the feasibility of utilizing low-salt wate ... | 2004 | 15344784 |
| genotypic and developmental evidence for the role of plasmodesmatal regulation in cotton fiber elongation mediated by callose turnover. | cotton fibers are single-celled hairs that elongate to several centimeters long from the seed coat epidermis of the tetraploid species (gossypium hirsutum and gossypium barbadense). thus, cotton fiber is a unique system to study the mechanisms of rapid cell expansion. previous work has shown a transient closure of plasmodesmata during fiber elongation (y.-l. ruan, d.j. llewellyn, r.t. furbank [2001] plant cell 13: 47-60). to examine the importance of this closure in fiber elongation, we compared ... | 2004 | 15557097 |
| genetic mapping and qtl analysis of fiber-related traits in cotton ( gossypium). | cotton, the leading natural fiber crop, is largely produced by two primary cultivated allotetraploid species known as upland or american cotton ( gossypium hirsutum l.) and pima or egyptian cotton ( g. barbadense l.). the allotetraploid species diverged from each other and from their diploid progenitors (a or d genome) through selection and domestication after polyploidization. to analyze cotton ad genomes and dissect agronomic traits, we have developed a genetic map in an f2 population derived ... | 2004 | 14513220 |
| widespread paleopolyploidy in model plant species inferred from age distributions of duplicate genes. | it is often anticipated that many of today's diploid plant species are in fact paleopolyploids. given that an ancient large-scale duplication will result in an excess of relatively old duplicated genes with similar ages, we analyzed the timing of duplication of pairs of paralogous genes in 14 model plant species. using est contigs (unigenes), we identified pairs of paralogous genes in each species and used the level of synonymous nucleotide substitution to estimate the relative ages of gene dupl ... | 2004 | 15208399 |
| comparative proteomics of cannabis sativa plant tissues. | comparative proteomics of leaves, flowers, and glands of cannabis sativa have been used to identify specific tissue-expressed proteins. these tissues have significantly different levels of cannabinoids. cannabinoids accumulate primarily in the glands but can also be found in flowers and leaves. proteins extracted from glands, flowers, and leaves were separated using two-dimensional gel electrophoresis. over 800 protein spots were reproducibly resolved in the two-dimensional gels from leaves and ... | 2004 | 15190082 |
| dexamethasone-inducible green fluorescent protein gene expression in transgenic plant cells. | genomic research has made a large number of sequences of novel genes or expressed sequence tags available. to investigate functions of these genes, a system for conditional control of gene expression would be a useful tool. inducible transgene expression that uses green fluorescent protein gene (gfp) as a reporter gene has been investigated in transgenic cell lines of cotton (cot; gossypium hirsutum l.), fraser fir [fra; abies fraseri (pursh) poir], nordmann fir (nor; abies nordmanniana lk.), an ... | 2004 | 15629039 |
| characterization of ghrac1 gtpase expressed in developing cotton (gossypium hirsutum l.) fibers. | cytoskeleton assembly plays an important role in determining cotton fiber cell length and morphology and is developmentally regulated. as in other plant cells, it is not clear how cytoskeletal assembly in fibers is regulated. recently, several rac/rop gtpases in arabidopsis were shown to regulate isotropic and polar cell growth of root hairs and pollen tubes by controlling assembly of the cytoskeleton. ghrac1, isolated from cottonseeds, is a member of the rac/rop gtpase family and is abundantly ... | 2004 | 15358513 |
| identification and quantification of gossypol in cotton by using packed micro-tips columns in combination with hplc. | self-packed micro-tip columns containing a c18-bonded silica stationary phase, based on the same principles as solid-phase extraction methods, were used to obtain gossypol and related sesquiterpenoid aldehyde-enriched fractions. the enriched metabolite fractions were then analyzed by optimized high-performance liquid chromatography (hplc) with a c18 column (4.6 mm x 25 cm) eluted with the binary mobile phase acetonitrile-0.1% aqueous tfa solution (80:20). this method has proven to be highly repr ... | 2004 | 15372136 |
| influence of cover crops on insect pests and predators in conservation tillage cotton. | in fall 2000, an on-farm sustainable agricultural research project was established for cotton, gossypium hirsutum l., in tift county, georgia. the objective of our 2-yr research project was to determine the impact of several cover crops on pest and predator insects in cotton. the five cover crop treatments included 1) cereal rye, secale cereale l., a standard grass cover crop; 2) crimson clover, trifolium incarnatum l., a standard legume cover crop; 3) a legume mixture of balansa clover, trifoli ... | 2004 | 15384330 |
| bollgard ii cotton: compositional analysis and feeding studies of cottonseed from insect-protected cotton (gossypium hirsutum l.) producing the cry1ac and cry2ab2 proteins. | bollgard ii cotton event 15985 producing the cry1ac and cry2ab2 proteins has been developed by genetic modification to broaden the spectrum of insects to which the plant is tolerant and to provide an insect resistance management tool to impede the onset of resistance. the purpose of this study was to evaluate the composition and nutrition of bollgard ii cotton, relative to the use for food and animal feed, compared to that of conventional cotton varieties. compositional analyses were conducted t ... | 2004 | 15537305 |
| boll injury and yield losses in cotton associated with brown stink bug (heteroptera: pentatomidae) during flowering. | brown stink bug, euschistus servus (say), was infested on cotton, gossypium hirsutum l., plants during reproductive stages to determine the effects on boll injury and seedcotton yield. during each week in 2002 and 2003, significantly more bolls with > or = 1 injured locule, bolls with > or = 2 injured locules, and bolls with discolored lint were recorded on stink bug-infested plants compared with that on noninfested plants. significantly fewer bolls displayed external injury on the boll exocarp ... | 2004 | 15666747 |
| [glyphosate-resistant cotton (gossypium hirsutum l.) transformed with aroam12 gene via agrobacterium tumefaciens]. | a mutant, aroam12, exhibiting resistance to glyphosate produced in a previous study using the staggered extension process with aroa genes from salmonella typhimurium and eschrichia coli. in this paper, we constructed a vector pgra1300 carrying aroam12 gene, comprising transit peptide of arabidopsis epsps, under the control of the camv35s promoter and used as selectable marker for cotton plant (gossypium hirsutum l.) transformation. transgenic cottons with increased resistance to glyphosate were ... | 2004 | 15599043 |
| effects of potassium humate solution and soaking periods on germination characteristics of undelinted cotton seeds (gossypium hirsutum l.). | the present study was conducted to determine the effects of soaking in potassium humate (ph) solution (55% humic acid, 30% fulvic acid and 8% potassium hydroxide) and distilled water (dw) for different periods (0, 4, 8 and 16 hours) on germination characteristics of undelinted seeds of cotton cv ersan-92. radicle, hypocotyl and seedling length, radicle and hypocotyl elongation rate and vigor index increased by ph solution treatment and increasing soaking periods. the medium x soaking period inte ... | 2004 | 15907066 |
| relative concentration of cry1a in maize leaves and cotton bolls with diverse chlorophyll content and corresponding larval development of fall armyworm (lepidoptera: noctuidae) and southwestern corn borer (lepidoptera: crambidae) on maize whorl leaf profiles. | to manage insect resistance to transgenic crops that express insecticidal proteins from bacillus thuringiensis (bt) berliner, the u.s. environmental protection agency recommends a refuge-based insect resistance management strategy where a percentage of non-bt (refuge) crop is grown in proximity to a bt-expressing crop. an important requirement for this strategy is that the toxin exists at a high effective dose for control of the target pest(s), so that heterozygous individuals in the population ... | 2004 | 15568367 |