Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| a highly unusual combination of pulmonary pathogens in an hiv infected patient. | we report on a case with an unusual combination of pulmonary pathogens including pneumocystis carinii, rhodococcus equi and toxoplasma gondii in an hiv infected patient. the multiple microbiological procedures required to ascertain the etiological diagnoses of pneumonitis in hiv-seropositive subjects are discussed. | 1994 | 8036478 |
| [pulmonary malacoplakia and rhodococcus equi pneumonia in a patient infected with the human immunodeficiency virus. a case report with review of the literature]. | we report a case of pulmonary malakoplakia in a patient suffering from aids secondary to a rhodococcus equi pneumonia. the association between these two pathologies only occurring in the immunodepressed does not seen fortuitous. deficiency in cellular immunity and macrophage cellular activity as well as failure of intracellular bactericidal and phagolysosomal function are very probably the links. the treatment of this opportunistic germ rests on prolonged poly-antibiotic therapy or indeed surgic ... | 1994 | 8041997 |
| cloning and sequence analysis of the gene encoding isocitrate lyase from rhodococcus fascians. | an isocitrate lyase (icl)-encoding gene (icl) from the gram+ plant pathogen rhodococcus fascians was identified serendipitously as part of a scrambled fragment after shotgun cloning in the promoter probe vector, pdp1. the icl protein is 429 amino acids long (47.11 kda) and has a predicted pi of 4.84; it is 54% similar to the escherichia coli icl and 24-27% to eukaryotic homologues. comparison of the prokaryotic and eukaryotic icl confirms the earlier proposal of matsuoka and mcfadden [j. bacteri ... | 1994 | 8045408 |
| animal-associated opportunistic infections among persons infected with the human immunodeficiency virus. | a number of animal-associated infections occur in persons infected with the human immunodeficiency virus (hiv), including those due to toxoplasma gondii, cryptosporidium, microsporida, salmonella, campylo-bacter, giardia, rhodococcus equi, rochalimaea, and listeria monocytogenes. most of these infections, with the exception of those due to rochalimaea, appear to be acquired by the immunosuppressed individual from sources other than exposure to animals. drs. glaser and colleagues review our curre ... | 1994 | 8054433 |
| enantioselective hydrolysis of racemic naproxen nitrile and naproxen amide to s-naproxen by new bacterial isolates. | bacteria were enriched from soil samples with succinate as a carbon source and racemic naproxen nitrile [2-(6-methoxy-2-naphthyl)propionitrile] as sole source of nitrogen. since naproxen nitrile was only poorly soluble in water media amended with different water-immiscible organic phases were used for the enrichments. with pristane (2,6,10,14-tetramethylpentadecane) as the organic phase two bacterial strains were isolated (strain c3ii and strain mp50) which were identified as rhodococci. cells o ... | 1994 | 7764731 |
| purification and characterization of a novel carbonyl reductase isolated from rhodococcus erythropolis. | during growth on n-tetradecane a novel nadh-dependent carbonyl reductase is induced in the gram-positive bacterium rhodococcus erythropolis (peters, p., zelinski, t. and kula, m.r. (1992) appl. microbiol. biotechnol. 38, 334-340). the enzyme has been purified to homogeneity using fractional ph precipitation, anion exchange chromatography and affinity chromatography. the isoelectric point of the oxidoreductase is 4.4. the apparent molecular mass of the native enzyme is 161 kda, that of the subuni ... | 1994 | 7764739 |
| production of a bioflocculant by mixed culture. | colony groups that form large amounts of slime externally were obtained from activated sludge among phthalate-assimilating microbes. that slime, which r-3 mixed microbes produced externally, had a high level of flocculation activity. r-3 mixed strains, one of such colony groups, efficiently produced a bioflocculant (apr-3) in liquid cultures of production medium, especially that containing starch and glucose (1:1) as carbon sources. identification of this group of bioflocculant-producing microbe ... | 1994 | 7765478 |
| nitrile hydratase gene from rhodococcus sp. n-774 requirement for its downstream region for efficient expression. | for improvement of the production of nitrile hydratase (nhase) from rhodococcus sp. n-774 by recombinant dna techniques, several plasmids, each of which had a deletion of the upstream or downstream region of the genes encoding the alpha and beta subunits of nhase, were constructed. enzyme assays of recombinant r. rhodochrous and escherichia coli cells showed that a downstream region of the nhase genes was indispensable for the production of active nhase in both cells, but for the production of t ... | 1994 | 7765511 |
| alpha-mannosidase: a rapid test for identification of arcanobacterium haemolyticum. | a 4-h alpha-mannosidase test for identification of arcanobacterium haemolyticum strains (n = 139) and differentiation of a. haemolyticum from actinomyces pyogenes strains (n = 30) and other gram-positive rods was evaluated. practically all a. haemolyticum strains (138 of 139) and the listeria monocytogenes type strain were alpha-mannosidase positive, while all a. pyogenes strains and corynebacterium (n = .25) strains as well as the rhodococcus equi and erysipelothrix rhusiopathiae type strains w ... | 1994 | 7710479 |
| infections due to rhodococcus equi in three hiv-infected patients: microbiological findings and antibiotic susceptibility. | infections of rhodococcus equi, a well-known pathogen in animals which causes cavitated pneumonia similar to that caused by mycobacteria, were studied in three hiv-infected patients. this microorganism was isolated in the bronchoalveolar washings of two patients and in the sputum of the third. in two patients, rh. equi represented the first clinical opportunistic manifestation of hiv disease. one patient died of concomitant pneumocystis infection. the eradication of the microorganism occurred in ... | 1994 | 7672872 |
| [rhodococcus equi pneumonia in patients with aids]. | 1994 | 7827773 | |
| rhodococcus equi: fatal pneumonia in a patient without aids. | rhodococcus equi infections are being recognized with greater frequency in immunocompromised patients and the population infected with the human immunodeficiency virus (hiv). r. equi usually causes a progressive pneumonia that may or may not cavitate and is relatively refractory to antibiotic therapy. we report a case of fatal r. equi pneumonia in a normal host. | 1994 | 7852068 |
| routine identification of mycobacterium tuberculosis complex isolates by automated hybridization. | methodologies for biochemical identification of mycobacteria isolated from clinical samples are still cumbersome, taking skilled technicians 3 to 6 weeks. we describe here a 2-h identification system for mycobacterial isolates belonging to the mycobacterium tuberculosis complex using a dna probe. after 30 min of hands-off sample preparation, the 1.5-h hybridization test is totally automated in the newly developed vidas system (biomérieux, marcyl'etoile, france), which performs solid-phase specif ... | 1994 | 7852560 |
| biochemical and serological characteristics of rhodococcus equi isolates from animals and humans. | in the present study, 17 rhodococcus equi isolates from animals and 13 r. equi isolates from humans were correctly identified by the use of the api coryne-test system. the biochemical characteristics revealed no significant differences between isolates from animals and humans. heat extractions at acid ph or autoclave extractions of the bacteria and the use of monospecific antisera against type antigens 1-7 allowed the serotyping of the bacteria. the majority of the r. equi isolates reacted with ... | 1994 | 7801718 |
| [rhodococcus equi pneumonia in patients with immunodeficiency]. | in three patients, men of 30, 21 and 24 years old respectively, rhodococcus equi pneumonia was diagnosed. antibiotic treatment, followed by secondary prophylaxis, was successful. in one patient, lobectomy was done. r. equi, a well-known bacterial pathogen in horses, appears also to be pathogenic in humans in case of immunodeficiency. | 1994 | 7808528 |
| [rhodococcus equi pneumonia in a patient with aids. primary resistance to imipenem]. | 1994 | 7811779 | |
| interaction of virulent and non-virulent rhodococcus equi human isolates with phagocytes, fibroblast- and epithelial-derived cells. | rhodococcus equi is a facultative, intracellular, gram-positive coccobacillus, increasingly reported in pneumonia of aids-infected patients. we investigated killing resistance properties of human r. equi virulent and avirulent human strains. avirulent beta-lactam-susceptible strains had lower intracellular colony forming units after 45 min incubation in murine macrophages j774 and human monocyte-macrophage tph-1 than those of virulent strains. only virulent beta-lactam-resistant strains persiste ... | 1994 | 7812268 |
| chlorocatechol 1,2-dioxygenase from rhodococcus erythropolis 1cp. kinetic and immunochemical comparison with analogous enzymes from gram-negative strains. | chlorocatechol 1,2-dioxygenase from rhodococcus erythropolis 1cp was purified to homogeneity. in contrast to chlorocatechol 1,2-dioxygenase from gram-negative strains which have a very broad substrate tolerance, the rhodococcus enzyme was relatively more specific and had a distinct preference for 4-substituted catechols. protein and metal analysis indicate an unusual stoichiometry of one atom each of iron and manganese/64-kda homodimer. the n-terminal amino acid sequence (27 residues) of the rho ... | 1994 | 7813460 |
| opacification of middlebrook agar as an aid in distinguishing nocardia farcinica within the nocardia asteroides complex. | among 58 aerobic actinomycetes isolated from different sources and geographical locations, none of 23 nocardia asteroides isolates, at 18 n. farcinica isolates, 1 of 5 n. otitidiscaviarum isolates, and 1 of 4 rhodococcus species isolates opacified middlebrook 7h10 medium. within the n. asteroides complex, this characteristic, together with growth at 45 degrees c and resistance to each of erythromycin, cefotaxime, and tobramycin, provides a simple means of distinguishing n. farcinica from n. aste ... | 1994 | 7814557 |
| rhodococcus equi pneumonia and pulmonary malakoplakia in acquired immunodeficiency syndrome. pathologic features. | pulmonary infection with rhodococcus equi is rare, and most cases are seen in immunocompromised patients, particularly those with acquired immunodeficiency syndrome. we describe the pathologic features in four cases of culture-positive r equi pneumonia occurring in patients infected with human immunodeficiency virus. all four patients had a solitary cavitary pulmonary mass that was resected (n = 3) or had undergone biopsy (n = 1). pathologically, all specimens revealed sheets of histiocytes with ... | 1994 | 7517660 |
| phylogenetic evidence for transfer of pentachlorophenol-mineralizing rhodococcus chlorophenolicus pcp-i(t) to the genus mycobacterium. | we determined the nucleotide sequence of a 16s rrna gene of rhodococcus chlorophenolicus pcp-i(t) (= dsm 43826t) (t = type strain). sequence comparisons revealed that there was a close relationship between strain pcp-i(t) and strains belonging to the genus mycobacterium. the sequence data were used to construct a phylogenetic tree, which showed that mycobacterium chubuense is the closest relative of strain pcp-i(t). we propose that strain pcp-i(t) should be transferred to the genus mycobacterium ... | 1994 | 7520738 |
| a phylogeny of the genus nocardia deduced from the analysis of small-subunit ribosomal dna sequences, including transfer of nocardia amarae to the genus gordona as gordona amarae comb. nov. | according to phylogenetic analyses of nearly complete small-subunit ribosomal dna sequences, the genus nocardia should not comprise the two species nocardia petroleophila and nocardia amarae. n. amarae should be reassigned to the genus gordona as gordona amarae. all of the other nocardia species form a monophyletic unit, closely related to species of the genus rhodococcus. it is proposed to revive the name 'cmn' to comprise the genera corynebacterium, tsukamurella, mycobacterium, gordona, rhodoc ... | 1994 | 7545965 |
| phylogenetic analysis of mycolic acid-containing wall-chemotype iv actinomycetes and allied taxa by partial sequencing of ribosomal protein at-l30. | the phylogenetic relationships among 30 mycolic acid-containing wall chemotype iv actinomycete strains and 12 strains belonging to allied taxa were examined by determining the amino acid sequences of the ribosomal at-l30 proteins of these organisms. sequencing 20 n-terminal amino acids of at-l30 preparations revealed that the members of the genera containing mycolic acid-containing actinomycetes form two clusters; the first cluster contains the genera nocardia, rhodococcus, gordona, and tsukamur ... | 1995 | 7547284 |
| phylogenetic analysis of the genus corynebacterium based on 16s rrna gene sequences. | the 16s rrna gene sequences of 30 strains representing 23 validated corynebacterium species and 7 currently non-valid corynebacterium species were determined. these sequences were aligned with the sequences of other corynebacterium species and related actinomycete taxa. a comparative sequence analysis revealed that there is considerable phylogenetic depth and internal structure in the genus corynebacterium. turicella otitidis and the amycolate species corynebacterium amycolatum were located at t ... | 1995 | 7547291 |
| phylogeny of the genus corynebacterium deduced from analyses of small-subunit ribosomal dna sequences. | we determined almost complete small-subunit ribosomal dna sequences of 50 reference strains belonging to the genera corynebacterium, rhodococcus, and gordona and compared these sequences with previously published sequences. three phylogenetic methods (the neighbor-joining, maximum-likelihood, and maximum-parsimony methods), as well as a bootstrap analysis, were used to assess the robustness of each topology which we obtained. the results of comparative phylogenetic analyses confirmed that the ge ... | 1995 | 7547293 |
| comparison of mycobacterium 23s rrna sequences by high-temperature reverse transcription and pcr. | we describe a modified rrna sequence analysis method which we used to determine the phylogenetic relationships among 58 species belonging to the genus mycobacterium. we combined the sensitivity of the reverse transcriptase pcr for amplifying nanogram amounts of template rrna material with the elevated extension temperatures used for the thermostable dna polymerase from thermus thermophilus. a 70 degrees c reverse transcription extension step permitted improved read-through of highly structured r ... | 1995 | 7547304 |
| assignment of the bacterial agent of urinary calculus in young rats by the comparative sequence analysis of the 16s rrna genes of corynebacteria. | comparative 16s rrna gene sequencing was used to assign four isolates of spontaneous urinary calculus in young laboratory rats. the phylogenetic relationships among the rat isolates and selected species of corynebacteria were also inferred. based on the homology and evolutionary distance analysis, the 16s rrna genes of the rat isolates were almost identical with that of corynebacterium renale atcc 19412. also the results of the phylogenetic analysis showed a close relationship among the isolates ... | 1995 | 7548409 |
| quinoline 2-oxidoreductase and 2-oxo-1,2-dihydroquinoline 5,6-dioxygenase from comamonas testosteroni 63. the first two enzymes in quinoline and 3-methylquinoline degradation. | the enzymes catalysing the first two steps of quinoline and 3-methylquinoline degradation by comamonas testosteroni 63 were investigated. quinoline 2-oxidoreductase, which catalyses the hydroxylation of (3-methyl-)quinoline to (3-methyl-)2-oxo-1,2-dihydroquinoline, was purified to apparent homogeneity. the native enzyme, with a molecular mass of 360 kda, is composed of three non-identical subunits (87, 32, and 22 kda), occurring in a ratio of 1.16:1:0.83. containing fad, molybdenum, iron, and ac ... | 1995 | 7556204 |
| activation of protein kinase c by mycobacterial cord factor, trehalose 6-monomycolate, resulting in tumor necrosis factor-alpha release in mouse lung tissues. | cord factors are mycoloyl glycolipids in cell walls of bacteria belonging to actinomycetales, such as mycobacterium, nocardia and rhodococcus. they induce granuloma formation in the lung and interstitial pneumonitis, associated with production of macrophage-derived cytokines. we studied how cord factors induce biological activities in the cells. cord factors isolated from m. tuberculosis, trehalose 6-monomycolate (mtmm) and trehalose 6,6'-dimycolate (mtdm), enhanced protein kinase c (pkc) activa ... | 1995 | 7559098 |
| plasmid prtl1 controlling 1-chloroalkane degradation by rhodococcus rhodochrous ncimb13064. | rhodococcus rhodochrous ncimb13064 can dehalogenate and use a wide range of 1-haloalkanes as sole carbon and energy source. the 1-chloroalkane degradation phenotype may be lost by cells spontaneously or after treatment with mitomycin c. two laboratory derivatives of the original strain exhibited differing degrees of stability of the chloroalkane degradation marker. plasmids of approximately 100 kbp (prtl1) and 80 kbp (prtl2) have been found in r. rhodochrous ncimb13064. prtl1 was shown to be car ... | 1995 | 7568468 |
| the sulphydryl-activated cytolysin and a sphingomyelinase c are the major membrane-damaging factors involved in cooperative (camp-like) haemolysis of listeria spp. | the negative mutant approach was used in this study to identify listerial cytolytic factors involved in cooperative haemolysis (camp-like phenomenon) with staphylococcus aureus and rhodococcus equi. a listeria monocytogenes non-haemolytic mutant specifically impaired in listeriolysin o (llo) production gave no camp reaction with s. aureus, and was virtually camp-negative with r. equi, indicating that the listerial sulphydryl-activated toxin played a major role in cooperative haemolysis. this was ... | 1995 | 7569324 |
| sequence and molecular characterization of a dna region encoding the dibenzothiophene desulfurization operon of rhodococcus sp. strain igts8. | dibenzothiophene (dbt), a model compound for sulfur-containing organic molecules found in fossil fuels, can be desulfurized to 2-hydroxybiphenyl (2-hbp) by rhodococcus sp. strain igts8. complementation of a desulfurization (dsz) mutant provided the genes from rhodococcus sp. strain igts8 responsible for desulfurization. a 6.7-kb taqi fragment cloned in escherichia coli-rhodococcus shuttle vector prr-6 was found to both complement this mutation and confer desulfurization to rhodococcus fascians, ... | 1995 | 7574582 |
| multiple genes encoding 2,3-dihydroxybiphenyl 1,2-dioxygenase in the gram-positive polychlorinated biphenyl-degrading bacterium rhodococcus erythropolis ta421, isolated from a termite ecosystem. | rhodococcus erythropolis ta421 was isolated from a termite ecosystem and is able to degrade a wide range of polychlorinated biphenyl (pcb) congeners. genetic and biochemical analyses of the pcb catabolic pathway of this organism revealed that there are four different bphc genes (bphc1, bphc2, bphc3, and bphc4) which encode 2,3-dihydroxybiphenyl dioxygenases. as determined by southern hybridization, none of the bphc genes exhibits homology to any other bphc gene. bphc1, bphc2, and bphc4 encode en ... | 1995 | 7574595 |
| characterization of the rhodococcus sp. ni86/21 gene encoding alcohol: n,n'-dimethyl-4-nitrosoaniline oxidoreductase inducible by atrazine and thiocarbamate herbicides. | a protein with a mol.mass of 51,000 (thce) that was induced in rhodococcus sp. ni86/21 during assimilation of thiocarbamate herbicides, atrazine, ethanol, propanol, glycerol, propionaldehyde or ethanolamine was identified by two-dimensional electrophoresis. the thce gene was cloned and sequenced. the deduced amino acid sequence revealed thce as a member of group iii alcohol dehydrogenases. thce displayed strong homology with sequenced subunit fragments of the homodecameric n,n'-dimethyl-4-nitros ... | 1995 | 7575099 |
| an improved escherichia coli-rhodococcus shuttle vector and plasmid transformation in rhodococcus spp. using electroporation. | the genetic studies of metabolically diverse rhodococcus spp. have been hampered by the lack of a system of introducing exogenous dna. the authors improved an existing escherichia coli-rhodococcus shuttle vector (pmvs301) by removing much of the dna not needed for replication and adding a multicloning site. this improved vector (pbs305) is 7.9 kb in length. its ability to transform rhodococcus was tested using electroporation parameters optimized for introduction of pmvs301 into rhodococcus. tra ... | 1995 | 7576519 |
| dehalogenation of haloalkanes by rhodococcus erythropolis y2. the presence of an oxygenase-type dehalogenase activity complements that of an halidohydrolase activity. | rhodococcus erythropolis y2 produced two types of dehalogenase: a hydrolytic enzyme, that is an halidohydrolase, which was induced by c3 to c6 1-haloalkane substrates, and at least one oxygenase-type dehalogenase induced by c7 to c16 1-haloalkanes and n-alkanes. the oxygenase-type activity dehalogenated c4 to c18 1-chloroalkanes with an optimum activity towards 1-chlorotetradecane. the halidohydrolase catalysed the dehalogenation of a wide range of 1- and alpha,omega-disubstituted haloalkanes an ... | 1995 | 7579998 |
| pathogenesis of rhodococcus equi infection in mice: roles of virulence plasmids and granulomagenic activity of bacteria. | virulence of rhocococcus equi atcc 33701 and its plasmid-cured derivative atcc 33701p- was compared in balb/c and c3h/hej mice in terms of bacterial growth kinetics and histological changes in the liver, spleen and lungs, and humoral immune responses. injection with a sublethal dose of 10(6) atcc 33701 in mice resulted in microabscess formation after rapid multiplication in the liver and spleen by day 4, and then the bacteria were gradually eliminated with the formation of granuloma and the prod ... | 1995 | 7581269 |
| the first characterization of a eubacterial proteasome: the 20s complex of rhodococcus. | the 26s proteasome is the central protease of the ubiquitin-dependent pathway of protein degradation. the proteolytic core of the complex is formed by the 20s proteasome, a cylinder-shaped particle that in archaebacteria contains two different subunits (alpha and beta) and in eukaryotes contains fourteen different subunits (seven of the alpha-type and seven of the beta-type). | 1995 | 7583123 |
| antimicrobial activity of cefotaxime tested against infrequently isolated pathogenic species (unusual pathogens). | the cefotaxime sodium spectrum of activity is very broad and includes many common species and a variety of less frequently isolated pathogens. we have reviewed the clinical microbiology literature (44 references) and the data base of the university of iowa hospitals and clinics (iowa city, ia) to collect data on the activity of cefotaxime against the less common species. cefotaxime was consistently active against actinobacillus actinomycetemcomitans, capnocytophaga spp., eikenella corrodens, ery ... | 1995 | 7587049 |
| sequence and expression of the bpdc1c2bade genes involved in the initial steps of biphenyl/chlorobiphenyl degradation by rhodococcus sp. m5. | the nucleotide (nt) sequence of the bpdc1c2bade genes which encode the first three enzymes in the biphenyl (bp) degradation pathway of gram+ rhodococcus sp. m5 (formerly arthrobacter m5) was determined. except for the ferredoxin component (bpdb) of the initial bp dioxygenase, the predicted amino acid (aa) sequences of the remaining proteins are found to be more closely related to the counterpart proteins (todc1c2bade) present in the toluene-degrader, pseudomonas putida f1, than those of three bp ... | 1995 | 7590299 |
| cloning and expression of the s-triazine hydrolase gene (trza) from rhodococcus corallinus and development of rhodococcus recombinant strains capable of dealkylating and dechlorinating the herbicide atrazine. | we used degenerate oligodeoxyribonucleotides derived from the n-terminal sequence of the s-triazine hydrolase from rhodococcus corallinus nrrl b-15444r in an amplification reaction to isolate a dna segment containing a 57-bp fragment from the trza gene. by using the nucleotide sequence of this fragment, a nondegenerate oligodeoxyribonucleotide was synthesized and used to screen a genomic library of r. corallinus dna for fragments containing trza. a 5.3-kb psti fragment containing trza was cloned ... | 1995 | 7592318 |
| identification of virulence-associated antigens and plasmids in rhodococcus equi from patients with aids. | rhodococcus equi is an emerging opportunistic pathogen of human immunodeficiency virus-infected patients. however, little is known about the characteristics of r. equi isolates from humans. this study characterized the plasmid content, expression of a virulence-associated antigen, and mouse virulence of 19 r. equi isolates from patients with and without aids. ecori digestion patterns and southern, western, and virulence analyses of these isolates with cryptic plasmids allowed definition of a new ... | 1995 | 7594668 |
| case reports: pericarditis and lymphadenitis due to rhodococcus equi. | most patients with rhodococcus equi infection are immunocompromised by either hiv infection, malignancy, or medication. diagnosis is frequently missed or delayed because the organisms, resembling diphtheroids on smears, may be regarded as contaminants. their clinical, pathologic, histochemical, and microbiologic resemblance to mycobacteria can result in misdiagnosis. two cases were seen recently in our institution. r. equi pericarditis developed in a 29-year-old woman with failed renal transplan ... | 1995 | 7604837 |
| pseudomonas aeruginosa aliphatic amidase is related to the nitrilase/cyanide hydratase enzyme family and cys166 is predicted to be the active site nucleophile of the catalytic mechanism. | a database search indicated homology between some members of the nitrilase/cyanide hydratase family, pseudomonas aeruginosa and rhodococcus erythropolis amidases and several other proteins, some of unknown function. block and profile searches confirmed these relationships and showed that four regions of the p. aeruginosa amidase had significant homology with corresponding regions of nitrilases. a phylogenetic tree placed the p. aeruginosa and r. erythropolis amidases in a group with nitrilases b ... | 1995 | 7607322 |
| cytokine modulation alters pulmonary clearance of rhodococcus equi and development of granulomatous pneumonia. | rhodococcus equi, a facultative intracellular bacterium, causes chronic, often fatal granulomatous pneumonia in young horses and in humans with aids. the inability of host alveolar macrophages to kill intracellular r. equi results in the development of granulomas and progressive loss of pulmonary parenchyma. clearance of the organism from the lung requires functional cd4+ t cells. the purpose of this study was to identify the cytokine effector mechanisms that mediate clearance of r. equi from th ... | 1995 | 7622227 |
| [pneumonia and bone marrow infection with rhodococcus equi in an aids patient]. | 1995 | 7623501 | |
| [susceptibility of bacterial isolates from the equine respiratory tract to trimethoprim, sulfadoxine, sulfadimethoxine and combinations of these compounds]. | using a broth microdilution technique, the in vitro susceptibility of bacterial isolates from the equine respiratory tract to trimethoprim, sulfadoxine, sulfadimethoxine, and combinations of these compounds was determined. the bacterial strains (n = 88) isolated recently from horses with respiratory symptoms belonged to the following species: streptococcus equi subsp. zooepidemicus (n = 34), streptococcus equi subsp. equi (n = 22), staphylococcus aureus (n = 9), klebsiella pneumoniae (n = 7), rh ... | 1995 | 7624856 |
| another cause of endobronchial lesions found in hiv patients. | 1995 | 7634912 | |
| characterization by arbitrary primer polymerase chain reaction of polychlorinated biphenyl (pcb)-degrading strains of comamonas testosteroni isolated from pcb-contaminated soil. | in this study, we isolated and characterized biphenyl (bp) and polychlorinated biphenyl (pcb) degrading bacterial strains found in pcb-contaminated soil from an auto manufacturing plant located in syracuse, new york. twenty-one bp and pcb-degrading bacteria were randomly selected to form a representative sample of the bacterial population present at the site. of the 21 bacteria, 13 were identified as comamonas testosteroni, constituting about 60% of the bacterial population examined. other pcb d ... | 1995 | 7641143 |
| aliphatic nitrilase from a soil-isolated comamonas testosteroni sp.: gene cloning and overexpression, purification and primary structure. | an aliphatic nitrilase, active on adiponitrile and cyanovaleric acid, was identified and purified from comamonas testosteroni sp. (ct). oligodeoxyribonucleotide probes were designed from limited amino acid (aa) sequence information and used to clone the corresponding gene, named nita. high homologies were found at the aa level between ct nitrilase and the sequences of known nitrilases. multi-alignment of sequenced nitrilases suggests that cys163 of ct plays an essential role in the active site. ... | 1995 | 7642130 |
| identification and activity of two insertion sequence elements in rhodococcus sp. strain igts8. | two putative insertion sequence (is) elements, is1166 and is1295, were identified on a plasmid present in rhodococcus sp. igts8. four copies of is1166 were present in strain igts8: one copy on each of two separate plasmids and two copies on a third plasmid or in the chromosome. of eight rhodococci tested, only r. zopfii contained a copy of an is1166-like element. two mutants of strain igts8 were isolated in which an additional copy of is1166 was present, suggesting that at least one copy of this ... | 1995 | 7642133 |
| identification of two new genes in the pseudomonas aeruginosa amidase operon, encoding an atpase (amib) and a putative integral membrane protein (amis). | the nucleotide sequence of the amidase operon of pseudomonas aeruginosa has been completed and two new genes identified amib and amis. the complete gene order for the operon is thus amiebcrs. the amib gene encodes a 42-kda protein containing an atp binding motif that shares extensive homology with the clp family of proteins and also to an open reading frame adjacent to the amidase gene from rhodococcus erythropolis. deletion of the amib gene has no apparent effect on inducible amidase expression ... | 1995 | 7642533 |
| a single cytochrome p-450 system is involved in degradation of the herbicides eptc (s-ethyl dipropylthiocarbamate) and atrazine by rhodococcus sp. strain ni86/21. | during atrazine degradation by rhodococcus sp. strain n186/21, n-dealkylated metabolites and an hydroxyisopropyl derivative are produced. the cytochrome p-450 system that is involved in degradation of thiocarbamate herbicides by strain n186/21 (i. nagy, g. schoofs, f. compernolle, p. proost, j. vanderleyden, and r. de mot, j. bacteriol. 177:676-687, 1995) is also required for atrazine degradation. atrazine-degrading activity was conferred on the atrazine-negative strains, mutant faj2027 of rhodo ... | 1995 | 7646049 |
| cloning of the genes for degradation of the herbicides eptc (s-ethyl dipropylthiocarbamate) and atrazine from rhodococcus sp. strain te1. | the degradation of the herbicides eptc (s-ethyl dipropylthiocarbamate) and atrazine (2-chloro-4-ethyl-amino-6-isopropylamino-1,3,5-triazine) is associated with an indigenous plasmid in rhodococcus sp. strain te1. plasmid dna libraries of rhodococcus sp. strain te1 were constructed in a rhodococcus-escherichia coli shuttle vector, pbs305, and transferred into rhodococcus sp. strain te3, a derivative of rhodococcus sp. strain te1 lacking herbicide degradation activity, to select transformants capa ... | 1995 | 7646050 |
| identification of virulent rhodococcus equi by amplification of gene coding for 15- to 17-kilodalton antigens. | during a survey of the prevalence of virulent rhodococcus equi at horse-breeding farms by plasmid and protein profiles, cryptic plasmids of various sizes were found in 66 (3.8%) of 1,725 isolates from feces of horses and 129 (5.9%) of 2,200 isolates from soil. twenty-two isolates, which contained cryptic plasmids of different sizes, were found by plasmid profiles, and their protein profiles and mouse pathogenicities were examined. of the 22 isolates, 7 were virulent r. equi, contained both virul ... | 1995 | 7650199 |
| development of a new selective plating medium for rhodococcus equi. | a new selective plating medium for rhodococcus equi containing ceftazidime (20 mg/l) and novobiocin (25 mg/l) on a mueller-hinton agar basis is described. it proved to be less inhibitory for r. equi than selective plating media devised earlier and grew only very few other nocardioform bacteria. | 1995 | 7651242 |
| machine operator's lung. a hypersensitivity pneumonitis disorder associated with exposure to metalworking fluid aerosols. | six auto parts manufacturing workers were referred for evaluation of a 6-week history of work-related dyspnea, cough, and fatigue. two workers also reported fever and weight loss. all six worked in a machining area where a waterbased metalworking fluid was used and recirculated under high pressure, thereby creating an aerosol. chest radiographs revealed pulmonary interstitial infiltrates in four workers. lung function tests showed that four workers had decreased diffusing capacity. after removal ... | 1995 | 7656609 |
| clinical pharmacologic aspects of cefixime in dogs. | the minimal inhibitory concentration (mic) of cefixime, a new third-generation orally administered caphalosporin, was determined for reference and clinical isolates from dogs. the mic of the drug for all but 1 of the 18 enterobacteriaceae isolates tested, 1 pasteurella canis, 1 rhodococcus equi, 1 streptococcus canis, and 1 streptococcus group g isolate, was less than 1.0 microgram/ml. the mic for 9 staphylococcus intermedius isolates ranged from 1.56 to 6.25 micrograms/ml and, for 8 sta aureus ... | 1995 | 7661460 |
| bacterial steroid monooxygenase catalyzing the baeyer-villiger oxidation of c21-ketosteroids from rhodococcus rhodochrous: the isolation and characterization. | steroid monooxygenase from rhodococcus rhodochrous, isolated in homogeneity with a high yield, catalyzes baeyer-villiger oxidation of progesterone to produce testosterone acetate with the stoichiometric consumptions of nadph and molecular oxygen. it is a flavoenzyme with the molecular size of 60 kda in the monomeric form and the isoelectric point of 4.9. the absorption spectrum has the maxima at 278, 376, and 439 nm and the shoulders at 360 and 465 nm, indicating a strong hypsochromic shift (blu ... | 1995 | 7669800 |
| a phylogenetic analysis of the genus nocardia with 16s rrna gene sequences. | partial sequences of the 16s rrna genes of the type strains of nine species of the genus nocardia were determined following the isolation and cloning of the amplified genes. these sequences were aligned with the sequences of representatives of the genera corynebacterium, gordona, mycobacterium, rhodococcus, and tsukamurella, and phylogenetic trees were inferred by using the fitch-margoliash and neighbor-joining methods. the genus nocardia formed a distinct clade that was most closely associated ... | 1995 | 7537058 |
| rhodococcus equi-associated necrotizing lymphadenitis in a llama. | a case of rhodococcus equi-associated necrotizing lymphadenitis in a 2-year-old male llama is described. caseous necrosis, resembling macroscopically that seen in ovine caseous lymphadenitis, was observed diffusely in the tracheobronchial and mediastinal lymph nodes, and in an extensive lesion in the lungs. necrosis was present to a lesser extent in the spleen and hepatic and gastric lymph nodes. numerous bacteria-laden macrophages were present around the necrotic areas. the findings suggest tha ... | 1995 | 7490342 |
| sequence of the rhodococcus equi gene encoding the virulence-associated 15-17-kda antigens. | the nucleotide sequence of the rhodococcus equi gene encoding the virulence-associated 15-17-kda antigens, located on plasmid preat701, has been determined. the gene encodes a 19-kda protein of 189 amino acids, with an ala-rich leader signal sequence (ss). at least five ss peptidase cleavage sites were found in this region. the molecular diversity of 15-17-kda antigens might be attributed to the multiple ss peptidase cleavage sites. | 1995 | 7698659 |
| establishment of oil-degrading bacteria associated with cyanobacteria in oil-polluted soil. | a unique natural microbial cocktail with promising potential for remediating oil-polluted desert in the gulf region is reported. oil-degrading micro-organisms immobilized within dense cyanobacterial mats on oily coasts of the arabian gulf were successfully established in oil-contaminated sand. those micro-organisms biodegraded 50% of the oil within 10-20 weeks. nocardioforms belonging to the genus rhodococcus predominated in the first few weeks, but after 22 weeks pseudomonas spp. increased, sha ... | 1995 | 7698954 |
| molecular characterization of a lipid-modified virulence-associated protein of rhodococcus equi and its potential in protective immunity. | virulent strains of rhodococcus equi produce plasmid-mediated 15- and 17-kda proteins, which are thermoregulated and apparently surface-expressed. we demonstrated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page) that r. equi produce three antigenically-related virulence-associated proteins, a diffuse 18-22-kda, a 17.5-kda and a 15-kda protein. phase partitioning of whole cells of r. equi strain 103 with triton x-114 (tx-114) and labelling with [3h]-labelled palmitic acid s ... | 1995 | 7704843 |
| photosensitive nitrile hydratase intrinsically possesses nitric oxide bound to the non-heme iron center: evidence by fourier transform infrared spectroscopy. | nitrile hydratase (nhase) from rhodococcus sp. n-771 is a photosensitive enzyme that catalyzes hydration of nitriles to the corresponding amides. light-induced fourier transform infrared difference spectra between the inactive and active forms of nhase were measured with both the natural (14n) and 15n-labeled nhases. the results showed, for the first time, that nhase intrinsically possesses nitric oxide (no) molecules bound to the non-heme iron center. the possible role of no in the photoactivat ... | 1995 | 7821438 |
| primary subcutaneous abscess caused by rhodococcus equi. | 1995 | 7825776 | |
| amino acid sequence analysis of ribosomal protein at-l30 from members of the family pseudonocardiaceae. | the phylogenetic relationships of the genera belonging to the family pseudonocardiaceae were examined by a novel approach, amino acid sequencing of ribosomal at-l30 proteins. the results of partial amino acid sequencing of at-l30 preparations revealed that the members of the family pseudonocardiaceae are divided into four clusters; the first cluster contains the genus actinopolyspora, the second cluster contains the genus saccharopolyspora, the third cluster contains the genus amycolatopsis, and ... | 1995 | 7857791 |
| dietzia, a new genus including dietzia maris comb. nov., formerly rhodococcus maris. | sequencing of the 16s ribosomal dnas (rdna) of two strains of rhodococcus maris was performed to determine the relationship of this species to other mycolic acid-containing actinomycetes. for this purpose we also determined the 16s rdna sequences for the type species of the genus rhodococcus, rhodococcus rhodochrous, and for mycobacterium chlorophenolicum (formerly rhodococcus chlorophenolicus), rhodococcus erythropolis, gordona bronchialis, and gordona terrae, for which only partial sequence da ... | 1995 | 7857805 |
| degradation of the thiocarbamate herbicide eptc (s-ethyl dipropylcarbamothioate) and biosafening by rhodococcus sp. strain ni86/21 involve an inducible cytochrome p-450 system and aldehyde dehydrogenase. | determination of the n-terminal sequences of two eptc (s-ethyl dipropylcarbamothioate)-induced proteins from thiocarbamate-degrading rhodococcus sp. strain ni86/21 resolved by two-dimensional electrophoresis enabled the localization of the respective structural genes on two distinct dna fragments. one of these strongly induced proteins is a nad(+)-dependent dehydrogenase active on aliphatic aldehydes. the second protein was identified as a cytochrome p-450 enzyme. the cytochrome p-450 gene repre ... | 1995 | 7836301 |
| rhodococcus meningitis in an immunocompetent host. | rhodococcus species are increasingly being recognized as pathogens, especially in patients infected with the human immunodeficiency virus. most cases of rhodococcus infection in these patients are due to rhodococcus equi and involve the lungs. cns infections due to rhodococcus species are rare, and meningitis due to non-equi rhodococcus has never been reported in a healthy host. we report a case of meningitis due to non-equi rhodococcus in a previously healthy 24-year-old woman. we also review a ... | 1995 | 7727645 |
| analysis of a 24-kilodalton protein associated with the polyhydroxyalkanoic acid granules in alcaligenes eutrophus. | a 5.0-kbp genomic ecori restriction fragment which complemented a third subclass of polyhydroxyalkanoic acid (pha)-leaky mutants of a. eutrophus that accumulated pha at a lower rate than the wild type was cloned from alcaligenes eutrophus h16. a 687-bp phapae gene on this fragment encoded a 24-kda protein (m(r) = 23,963), which was referred to as the ga24 protein. the ga24 protein was solubilized from the granules and purified to electrophoretic homogeneity, and antibodies against the ga24 prote ... | 1995 | 7730274 |
| identification of the region of a 14-kilodalton protein of rhodococcus ruber that is responsible for the binding of this phasin to polyhydroxyalkanoic acid granules. | the function of the polyhydroxyalkanoic acid (pha) granule-associated ga14 protein of rhodococcus ruber was investigated in escherichia coli xl1-blue, which coexpressed this protein with the polyhydroxybutyric acid (phb) biosynthesis operon of alcaligenes eutrophus. the ga14 protein had no influence on the biosynthesis rate of phb in e. coli xl1-blue(pskco7), but this recombinant e. coli strain formed smaller phb granules than were formed by an e. coli strain that expressed only the phb operon. ... | 1995 | 7730285 |
| the evolutionary relationship of biphenyl dioxygenase from gram-positive rhodococcus globerulus p6 to multicomponent dioxygenases from gram-negative bacteria. | the gram+ bacterium rhodococcus globerulus p6 (rgp6) catabolizes a range of polychlorinated biphenyl (pcb) congeners, thus being of interest in bioelimination processes for pcb. the first step in the pathway, a dioxygenase attack of one of the biphenyl (bp) rings, is catalyzed by biphenyl dioxygenase (bdo). in this study, the nucleotide (nt) sequences of the four clustered cistrons, bpha1a2a3a4, encoding the subunits of bdo and forming part of the bph operon of rgp6 for bp degradation, were dete ... | 1995 | 7737502 |
| rhodococcus equi--an increasingly recognized opportunistic pathogen. report of 12 cases and review of 65 cases in the literature. | rhodococcus equi, a gram-positive, weakly acid-fast coccobacillus, initially isolated from horses, is becoming increasingly recognized as an important pathogen for immunosuppressed human hosts since the first human case was reported in 1967. a review of the english medical literature yielded 53 cases. during the last 11 years, the microbiology laboratories of the authors isolated the organism from 12 patients. of the total 65 cases, 60 occurred in immunosuppressed patients with hiv infection, ma ... | 1995 | 7741114 |
| disseminated abscesses caused by rhodococcus equi in a patient with chronic lymphocytic leukemia. | 1995 | 7742468 | |
| characterization of the bacillus stearothermophilus br219 phenol hydroxylase gene. | the catabolic genes phea and pheb, coding for the conversion of phenol to catechol and catechol to 2-hydroxymuconic semialdehyde, respectively, have been cloned from bacillus stearothermophilus br219 into escherichia coli. following its localization on the 11-kb b. stearothermophilus dna insert by deletion and expression analysis, the phenol hydroxylase gene phea was subcloned as a 2-kb hindiii fragment, whose transformant expressed the enzyme after phenol induction and even more strongly after ... | 1995 | 7747948 |
| cauda equina syndrome, diskospondylitis, and a paravertebral abscess caused by rhodococcus equi in a foal. | a 4-month-old male quarter horse was referred for evaluation of urinary incontinence. physical examination revealed clinical signs consistent with cauda equina syndrome. radiography revealed diskospondylitis of s2 through s4. infected bone was surgically curretted, and drainage was established for an associated paravertebral abscess. rhodococcus equi was isolated from specimens of bone and from fluid samples obtained from the paravertebral abscess. bethanechol was administered to stimulate urina ... | 1995 | 7751225 |
| characterization of muconate and chloromuconate cycloisomerase from rhodococcus erythropolis 1cp: indications for functionally convergent evolution among bacterial cycloisomerases. | muconate cycloisomerase (ec 5.5.1.1) and chloromuconate cycloisomerase (ec 5.5.1.7) were purified from extracts of rhodococcus erythropolis 1cp cells grown with benzoate or 4-chlorophenol, respectively. both enzymes discriminated between the two possible directions of 2-chloro-cis, cis-muconate cycloisomerization and converted this substrate to 5-chloromuconolactone as the only product. in contrast to chloromuconate cycloisomerases of gram-negative bacteria, the corresponding r. erythropolis enz ... | 1995 | 7751292 |
| chronic osteitis due to rhodococcus equi in an immunocompetent patient. | 1995 | 7756506 | |
| [the fist detection of giardia spp. in horses in the czech republic]. | the first occurrence of giardia spp. in horses in the czech republic is reported. during preventive examination of 360 five-month up to 14-year horses from various parts of the region of central bohemia carried out from january 1993 to june 1994 in the parasitological laboratory of the state veterinary institute in prague, the giardia cysts were detected in the excrements of 18 (5%) horses, mostly 2-4 years of age, and in two foals 3 and 6 weeks old. during the period between march 1993 and june ... | 1995 | 7762123 |
| radiologic findings in two aids patients with rhodococcus equi pneumonia. | rhodococcus equi (r. equi) has been reported as an occasional cause of pulmonary infection in severely immunocompromised hosts, including aids patients. our purpose is to describe the radiologic findings in two aids patients with r. equi pneumonia. chest radiographs showed right-upper-lobe consolidation and cavitation in both patients. chest ct confirmed upper mediastinal involvement and precarinal lymphadenopathy in both cases. multiple lung nodules related to the bronchi were also identified i ... | 1995 | 7769626 |
| metabolism of halohydroquinones in rhodococcus chlorophenolicus pcp-1. | the actinomycete rhodococcus chlorophenolicus pcp-1 metabolizes pentachlorophenol into ultimate inorganic end products via tetrachloro-p-hydroquinone. this intermediate was further dehalogenated in the cytoplasm requiring reductant in the cell free system. tetrafluoro-p-hydroquinone and tetrabromo-p-hydroquinone were also dehalogenated. chlorophenol analogs, thiol blocking agents and molecular oxygen inhibited the activity. the dehalogenating reactions led to 1,2,4-trihydroxybenzene, which was f ... | 1995 | 7772938 |
| rhodococcal abortion and pneumonia in an equine fetus. | 1995 | 7779954 | |
| distribution and characterization of beta-lactamases of mycobacteria and related organisms. | the detailed distribution and precise features of mycobacterial beta-lactamases urgently need to be elucidated. | 1995 | 7780097 |
| what is your diagnosis? osteomyelitis, septic arthritis. | 1995 | 7782239 | |
| characterization of biphenyl catabolic genes of gram-positive polychlorinated biphenyl degrader rhodococcus sp. strain rha1. | rhodococcus sp. strain rha1 is a gram-positive polychlorinated biphenyl (pcb) degrader which can degrade 10 ppm of pcb48 (equivalent to aroclor1248), including tri-, tetra-, and pentachlorobiphenyls, in a few days. we isolated the 7.6-kb ecori-bamhi fragment carrying the biphenyl catabolic genes of rha1 and determined their nucleotide sequence. on the basis of deduced amino acid sequence homology, we identified six bph genes, bpha1a2a3a4, bphb, and bphc, that are responsible for the initial thre ... | 1995 | 7793929 |
| active-site analysis of ferric p450 enzymes: hydrogen-bonding effects on the circular dichroism spectra. | active-site cd spectra were analyzed for p450's with known x-ray structures (p450terp, p450bm-3, p450cam). cd spectral patterns for fe(3+)-substrate-free p450's reflect structure/function properties of the distal pocket. p450terp and p450bm-3 have an h-bond between 6th ligand and i-helix [hasemann, c.a., et al. (1995) structure, 3, 41-62], and the soret cd band at approximately 410 nm is approximately 2-fold larger than that at approximately 350 nm. for p450cam, the two cd bands are more nearly ... | 1995 | 7794248 |
| [nosocomial infections: new agents, incidence, prevention]. | nosocomial infection is one of the major health problems confronting clinicians in intensive care units. incidence has been estimated to vary from 5 to 10%, leading to death in one of every 5000 persons hospitalized. this situation is not however static since hospital epidemics and nosocomial infections evolve as a consequence of advances in antibiotic drug therapy and surgical and intensive care techniques. microbial investigations themselves are of major importance as the collection of epidemi ... | 1995 | 7899352 |
| cytologic appearance of rhodococcus equi in bronchoalveolar lavage specimens. a case report. | immunocompromised patients are at risk of developing infections caused by rhodococcus equi, a gram-positive bacillus that can cause pneumonia in patients with acquired immunodeficiency syndrome. to the author's knowledge this is the first report describing the cytologic features of the infection in a patient who had a confirmatory tissue biopsy and positive culture. infection with r equi may go unrecognized by pathologists unaware of its presentation and appearance in cytologic material. | 1995 | 7846998 |
| o-, m- and p-hydroxybenzoate degradative pathways in rhodococcus erythropolis. | rhodococcus erythropolis strain s1 uses the gentisate pathway to metabolize salicylate and m-hydroxybenzoate and the protocatechuate pathway to degrade p-hydroxybenzoate. m-hydroxybenzoate 6-hydroxylase was induced by growth on m-hydroxybenzoate or gentisate, and salicylate 5-hydroxylase only by growth on salicylate. p-hydroxybenzoate 3-hydroxylase could be induced only by growth on p-hydroxybenzoate. m-hydroxybenzoate or p-hydroxybenzoate could repress the induction of salicylate 5-hydroxylase. ... | 1995 | 7867918 |
| brevibacterium linens pbl33 and rhodococcus rhodochrous prc1 cryptic plasmids replicate in rhodococcus sp. r312 (formerly brevibacterium sp. r312). | the replication of two cryptic plasmids from brevibacterium linens atcc 9174 (pbl33) and rhodococcus rhodochrous atcc 4276 (prc1) was investigated in rhodococcus sp. r312 (formerly brevibacterium sp. r312). the recombinant plasmids psp33 (pbl33 derivative) and pspc1 (prc1 derivative) were found to be suitable for establishing new host-vector systems for rhodococcus sp. r312. they all carry the tn903 neomycin-resistance-encoding gene (aphi). | 1995 | 7867954 |
| isolation of insertion elements from gram-positive brevibacterium, corynebacterium and rhodococcus strains using the bacillus subtilis sacb gene as a positive selection marker. | the sacb gene of bacillus subtilis was successfully applied in various arthrobacter, brevibacterium, corynebacterium and rhodococcus strains for the isolation of transposable elements. three different insertion sequence (is) elements entrapped in sacb were isolated. the is elements is-bl and is-cg isolated from brevibacterium lactofermentum and corynebacterium glutamicum, respectively, were found to be similar in size (1.45 kb) and generated target duplications of 8 bp. their inverted repeats sh ... | 1995 | 7896070 |
| a physical map of the 85 kb virulence plasmid of rhodococcus equi 103. | a physical map of the 85 kb virulence plasmid pots from rhodococcus equi 103 was constructed. the restriction map contains 2 asni, 5 bglii, 9 ecori, 4 hindiii, and 3 xbai sites. the positions of the ecori and hindiii of pots are identical to that of the 85 kb virulence plasmid of r. equi atcc 33701 reported recently by others. ecori restriction fragment sizes were similar in the 85 kb plasmids isolated from 4 horse derived r. equi but, except apparently for the 28.3 and possibly 2.0 and 1.5 kb f ... | 1995 | 8521357 |
| sizing of the rhodococcus sp. r312 genome by pulsed-field gel electrophoresis. localization of genes involved in nitrile degradation. | the two restriction enzymes asni and drai were found to produce dna fragment sizes that could be used for mapping the rhodococcus sp. r312 (formerly brevibacterium sp. r312) genome by pulsed-field gel electrophoresis. asni produced 24 fragments (4 to 727 kb) and drai yielded 15 fragments (8.5 to 2400 kb). the fragment lengths in each digest were summed, indicating that the size of the chromosome ranged from 6.31 to 6.56 mb, with a mean of 6.44 mb. in addition, the wide-spectrum amidase gene (ami ... | 1995 | 8546455 |
| pathogenic nocardia isolated from clinical specimens including those of aids patients in thailand. | forty strains of nocardioform microorganisms were isolated as clinical specimens including several from aids patients in thailand. among them, 37 strains were found to belong to the genus nocardia. our identification studies revealed that most of the strains (25 strains) belong to the n. asteroides group, i.e., n. asteroides sensu stricto and n. farcinica. three strains were identified as n. otitidiscaviarum and two strains n. brasiliensis. in addition, 7 strains of rare pathogenic n. transvalen ... | 1995 | 8549723 |
| association with hela cells by rhodococcus equi with and without the virulence plasmid. | hela cell association was examined in eight rhodococcus equi isolates containing 80-85 kb plasmids and their plasmid negative derivatives, and in two other plasmid negative r. equi strains. seven of the eight plasmid positive strains possessed an 85 kb plasmid and produced the virulence-associated protein (vapa) detected by monoclonal antibody staining in immunoblots; one of the eight had an 80 kb plasmid but did not produce vapa. curing of the plasmids by repetitive subcultures at 42 degrees c ... | 1995 | 8560735 |
| sequential involvement of nk cells and cd8+ t cells in granuloma formation of rhodococcus aurantiacus-infected mice. | we investigated the effect of in vivo administration of antibodies against t-cell subsets and natural killer (nk) cells on endogenous gamma interferon (ifn-gamma) production and granuloma formation in rhodococcus aurantiacus-infected mice. high titers of endogenous ifn-gamma were detected in the extracts of the livers and spleens during 24 hr of the infection, reaching the peak at 8 hr, and the ifn-gamma production was reduced by in vivo administration of anti-nk 1.1 monoclonal antibody (mab) or ... | 1995 | 8569535 |
| pharmacokinetics and therapeutic potential for repeated oral doses of trimethoprim/sulphachlorpyridazine in horses. | the pharmacokinetic parameters of a powder formulation of trimethoprim/sulphachlorpyridazine were studied in eight healthy horses which received 5 mg/kg trimethoprim and 25 mg/kg sulphachlorpyridazine 12-hourly with concentrate for five days. the intake of the medicated concentrate by the horses was variable during the first two days, but after they became accustomed to the taste the intake by all the horses during the last three days was good. faecal samples taken before and on the last day of ... | 1995 | 8578661 |
| soil management enhancing hydrocarbon biodegradation in the polluted kuwaiti desert. | oil-polluted kuwaiti desert samples, exposed to the open air, were subjected to specific types of management, once every 2 weeks, throughout a year; control samples were not treated. the total amounts of extractable alkanes from the control samples remained fairly constant during the dry hot months, but decreased during the rainy months reaching, after 1 year, slightly more than one-half of the amount at zero time. this result demonstrates the self-cleaning of the kuwaiti desert and the essentia ... | 1995 | 8579833 |
| evaluation of the applicability of amplified rdna-restriction analysis (ardra) to identification of species of the genus corynebacterium. | the 16s rrna genes (rdna) of 50 strains belonging to 26 different coryneform bacterial species and genomospecies and of the type strain of rhodococcus equi were enzymatically amplified. amplified rdna restriction analysis (ardra) with the enzymes alui, cfoi and rsai was carried out. the combination of the ardra patterns obtained after restriction with these three different enzymes enabled the differentiation between the following species: corynebacterium accolens (number of strains = 2), c. afer ... | 1995 | 8584787 |