Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| colonization of streptococcus mutans and streptococcus sobrinus genotypes and caries development in children to mothers harboring both species. | the major route of early acquisition of mutans streptococci in humans is a vertical transmission from mother to child. the purpose of this longitudinal study was to examine the acquisition, distribution and persistence of streptococcus mutans and streptococcus sobrinus in children whose mothers harbored both species and to study the caries incidence in relation to colonization of these bacteria. fifteen mother-child pairs were followed during the child's first 7 years. stimulated salivary sample ... | 2004 | 14767165 |
| expression of the gtfi gene from streptococcus sobrinus in streptococcus anginosus using integration-mediated transformation system. | we have constructed a streptococcus anginosus transformant expressing the gtfi gene from streptococcus sobrinus, using a previously developed integration-mediated transformation system to introduce foreign genes onto the oral streptococcal chromosome, and attempted to evaluate the gene expression. in this system, one cloning plasmid and three pacyc184 derivatives, anchor, heterodimer, and integration plasmids were used for the construction of a series of integrants via homologous recombination. ... | 2004 | 15716022 |
| the influence of biosurfactants released by s. mitis bms on the adhesion of pioneer strains and cariogenic bacteria. | the influence of streptococcus mitis bms biosurfactants on the adhesion of eight pioneer and four cariogenic oral bacterial strains was, for a first screening, examined in a microtiter plate assay. the adhesion to pellicle-coated wells of three cariogenic strains was inhibited >70% by the biosurfactants, while only one pioneer strain showed >70% reduction. the reduction for the other strains did not exceed 50%. subsequently, adhesion of streptococcus mutans atcc 25175 and streptococcus sobrinus ... | 2004 | 15788225 |
| [mgb probes detect streptococcus mutans and streptococcus sobrinus in real time]. | to detect and distinguish streptococcus mutans (s. mutans) and streptococcus sobrinus (s. sobrinus) quickly in epidemiology and investigate the distribution of s. mutans in the oral of children with rampant caries. | 2005 | 15804014 |
| optimization of conditions for the efficient production of mutan in streptococcal cultures and post-culture liquids. | the strain streptococcus sobrinus ccug 21020 was found to produce water-insoluble and adhesive mutan. the factors influencing both stages of the mutan production, i.e. streptococcal cultures and glucan synthesis in post-culture supernatants were standardized. the application of optimized process parameters for mutan production on a larger scale made it possible to obtain approximately 2.2 g of water-insoluble glucan per 11 of culture supernate--this productivity was higher than the best reported ... | 2005 | 15813222 |
| immunological and protective effects of diepitopic subunit dental caries vaccines. | as a prelude to development of broader-spectrum vaccines for dental caries, we explored the immune potential of constructs combining epitopes from mutans streptococcal glucosyltransferases (gtf) and glucan binding protein b (gbpb). two diepitopic peptide constructs were synthesized in a multiple antigenic peptide (map) format. both constructs contained syi, a 20-mer gbpb peptide that included a sequence having major histocompatibility complex class ii binding characteristics. one diepitopic cons ... | 2005 | 15845483 |
| six-month polyol chewing-gum programme in kindergarten-age children: a feasibility study focusing on mutans streptococci and dental plaque. | to investigate the use of polyol-containing chewing gums in a day-care centre (kindergarten) setting as a means to affect the growth of mutans streptococci and dental plaque. | 2005 | 15880962 |
| chemical compositions and antimicrobial activities of four different anatolian propolis samples. | propolis means a gum that is gathered by bees from various plants. it is known for its biological properties, having antibacterial, antifungal and healing properties. the aims of this study were to evaluate the antimicrobial activity of four different anatolian propolis samples on different groups of microorganisms including some oral pathogens and comparison between their chemical compositions. ethanol extracts of propolis (eep) were prepared from four different anatolian propolis samples and e ... | 2005 | 15881836 |
| susceptibilities of periodontopathogenic and cariogenic bacteria to antibacterial peptides, {beta}-defensins and ll37, produced by human epithelial cells. | antimicrobial peptides are one of the factors involved in innate immunity. the susceptibility of periodontopathogenic and cariogenic bacteria to the major antimicrobial peptides produced by epithelia was investigated. | 2005 | 15886266 |
| effects of disinfectants on resilient denture-lining materials contaminated with staphylococcus aureus, streptococcus sobrinus, and candida albicans. | resilient denture-lining materials are more susceptible to microbial adhesion than are denture base acrylic resin. as denture hygiene is essential to maintain the serviceability of the denture, disinfectant solutions have been suggested as a method for effectively cleaning resilient liners. the aim of this study was to determine the effectiveness of four different disinfectant solutions on four different resilient lining materials contaminated with staphylococcus aureus, streptococcus sobrinus, ... | 2005 | 15892535 |
| in vitro and in vivo effects of isolated fractions of brazilian propolis on caries development. | recently, two chemically different types of brazilian propolis (type-3 and -12) were shown to have cariostatic properties. this study aimed to evaluate the influence of their isolated fractions on mutans streptococci viability, glucosyltransferases (gtfs) activity and caries development in rats. the ethanolic extracts of propolis (eeps) were serially fractionated into hexane (h-fr), chloroform, ethyl acetate, and ethanol. the ability of the four fractions and eep to inhibit streptococcus mutans ... | 2005 | 15913934 |
| longitudinal study of dental caries incidence associated with streptococcus mutans and streptococcus sobrinus in pre-school children. | streptococcus mutans and streptococcus sobrinus are known to be associated with the development of dental caries. in this study these bacteria were detected in pre-school children (each with primary dentition, age range 3-5 years, n = 60) using a pcr method, and then their presence was compared with the incidence of dental caries over a 1-year period. plaque samples were collected from all erupted tooth sites using a sterile toothbrush. dental examinations at the beginning of the study (baseline ... | 2005 | 15947431 |
| effect of acidulated phosphate fluoride (apf) gel on the adherence of cariogenic bacteria to resin composites. | to evaluate the effect of 1.23% acidulated phosphate fluoride (apf) and the subsequent polishing on the in vitro adherence of mutans streptococci to resin composites in a model that simulated the oral cavity conditions. | 2005 | 15973825 |
| salivary actinomyces naeslundii genospecies 2 and lactobacillus casei levels predict pregnancy outcomes. | gravida's poor periodontal health is emerging as a modifiable independent risk factor for preterm delivery and low birth weight. | 2005 | 15974839 |
| effects of sanguinaria in fluoride-containing dentifrices on the remineralisation of subsurface carious lesion in vitro. | the purpose of this study was to investigate the potential cariostatic benefits of dentifrice containing both fluoride (f) and sanguinaria (sg) in a silica abrasive formula using the microbial de- and re-mineralisation cycle model. | 2005 | 15997962 |
| the complex oral microflora of high-risk individuals and groups and its role in the caries process. | the involvement of the oral biofilm in the caries process requires re-evaluation. the essential role of mutans streptococci (streptococcus mutans and streptococcus sobrinus) in the caries process is not proven. acid production by dental plaque is not dependent upon the presence of mutans streptococci; caries occurs in the absence of these species and their presence does not necessarily indicate caries activity. other oral bacteria, non-mutans streptococci, actinomyces spp. and bifidobacterium sp ... | 2005 | 16008631 |
| antibacterial activities and bonding of mmsa/tbb resin containing amphiphilic lipids. | the purpose of this study was to investigate the antibacterial activity of mma/tbb resin containing newly developed amphiphilic lipids. the amphiphilic lipids, c10-l-ala/pts and c12-l-ala/pts, synthesized from the reaction of n-alkyl alcohol and l-alanine were dissolved in mma at concentrations of 0.5, 1.0, 1.5, and 2.0 mol%. resin mixtures of pmma powder and each mma liquid containing lipid and tbb were prepared for all tests. both lipids gave antibacterial effect to mma/ tbb resin. the additio ... | 2005 | 16022446 |
| effect of xylitol-containing chewing gums on interdental plaque-ph in habitual xylitol consumers. | the aim was to investigate the effect of high and low amounts of xylitol on the interdental plaque-ph, directly and after sucrose challenge, in schoolchildren with habitual consumption. | 2005 | 16040446 |
| tansmission, diversity and virulence factors of sreptococcus mutans genotypes. | dental caries is an infectious and transmissible disease, in which many genetic, environmental and behavioral risk factors interact. the mutans streptococci (ms), mainly streptococcus mutans and streptococcus sobrinus are the microorganisms most strongly associated with this disease. the main virulence factors associated with ms cariogenicity include adhesion, acidogenicity and acid tolerance. these properties work together to modify the physico-chemical properties of the biofilm, resulting in e ... | 2005 | 16050484 |
| low salivary iga activity to cell-surface antigens of mutans streptococci related to hla-drb1*04. | mutans streptococci are found in almost all individuals, though there are large differences in colonization levels between individuals. these differences are not readily explained, though several factors are believed to influence the colonization. one factor is the immune response to mutans streptococci, mainly provided by salivary immunoglobulin a (iga). in a previous study, differences in salivary iga reactions to oral streptococci were observed between human leukocyte antigen (hla)-dr4-positi ... | 2005 | 15720566 |
| antimicrobial activity of croton cajucara benth linalool-rich essential oil on artificial biofilms and planktonic microorganisms. | we have previously demonstrated that a linalool-rich essential oil from croton cajucara benth presents leishmanicidal activity. in the present study, we demonstrate that this essential oil inhibits the growth of reference samples of candida albicans, lactobacillus casei, staphylococcus aureus, streptococcus sobrinus, porphyromonas gingivalis and streptococcus mutans cell suspensions, all of them associated with oral cavity disease. the purified linalool fraction was only inhibitory for c. albica ... | 2005 | 15720570 |
| cranberry high molecular weight constituents promote streptococcus sobrinus desorption from artificial biofilm. | dental biofilm harbouring oral bacteria is highly correlated with the progression of dental diseases. disruption of biofilm formation via anti-adhesion agents is an alternative means to the antibacterial approach. previous studies have shown that high molecular weight non-dialysable material (ndm) derived from cranberry juice inhibits the adhesion of escherichia coli and the coaggregation of a variety of oral bacteria. in addition, it inhibits the formation of glucans and fructans synthesised by ... | 2005 | 15737520 |
| arbitrarily primed polymerase chain reaction for fingerprinting the genotype identification of mutans streptococci in children with down syndrome. | this study investigated the possible intrafamilial similarity of mutans streptococcal strains in some families with a child with down syndrome using chromosomal dna fingerprinting. the isolates were genotyped using arbitrarily primed polymerase chain reaction with the opa 02 and opa 03 primers. the results showed that five children with down syndrome harbored mutans streptococci genotypes different from those of their mothers. a matching of genotypes was observed within the control pair (mother/ ... | 2005 | 15779663 |
| levels of mutans streptococci and lactobacilli in plaque on aged restorations of an ion-releasing and a universal hybrid composite resin. | the purpose of this in vivo study was to evaluate the cariogenic microflora of plaque on aged restorations of a hydroxyl, fluoride, and calcium ion-releasing composite resin (ircr) (ariston phc), and to compare it intra-individually with a universal hybrid composite resin and enamel. each of 19 subjects received one proximal restoration of the ircr, one proximal universal hybrid composite resin restoration (cr) and each subject had one non-filled proximal enamel control surface to make intra-ind ... | 2005 | 16095058 |
| quantitative analysis of the adhesion of cariogenic streptococci to orthodontic metal brackets. | the aim of this study was to analyze the adhesion of cariogenic streptococci to orthodontic metal brackets in terms of the type of bacterial strains, the incubation time, and saliva coating. two strains of streptococcus mutans (s. mutans lm7 and s. mutans omz65) and two strains of s. sobrinus (s. sobrinus b13 and s. sobrinus 6715) were used. twenty metal brackets were incubated with either unstimulated whole saliva or phosphate-buffered saline for two hours. the bacterial adhesion assays were th ... | 2005 | 16097239 |
| differentiation of mutans streptococci by intact cell matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. | it is difficult to distinguish mutans streptococci on the species level, and even more so on the subspecies level. intact cell matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (maldi-tof-ms) (icm) was applied to reference strains of five of the species of the mutans group (streptococcus criceti, streptococcus downei, streptococcus mutans, streptococcus ratti, streptococcus sobrinus), nonmutans streptococci (streptococcus oralis, streptococcus mitis, streptococcus sali ... | 2005 | 16101961 |
| synergistic inhibition by combination of fluoride and xylitol on glycolysis by mutans streptococci and its biochemical mechanism. | the purpose of this study was to evaluate the combined inhibitory effect of fluoride and xylitol on acid production by mutans streptococci, streptococcus mutans nctc10449 and streptococcus sobrinus 6715, from glucose under strictly anaerobic conditions at fixed ph 5.5 and 7.0. the bacteria were grown in a tryptone-yeast extract broth under strictly anaerobic conditions (n2: 80%; h2: 10%; co2: 10%). reaction mixtures for acid production from glucose contained bacterial cells with fluoride (0-6.4 ... | 2005 | 16251799 |
| inhibitory activity of glass-ionomer cements on cariogenic bacteria. | this study evaluated the antibacterial activity of the glass-ionomer cements vitrebond (3m espe), ketac molar (3m espe) and fuji ix (gc america) against s mutans, s sobrinus, l acidophilus and a viscosus, using the agar diffusion test. inocula were obtained by the seed of indicators cultures in bhi broth incubated at 37 degrees c for 24 hours. base layers containing 15 ml of bhi agar and 300 microl of each bacteria suspension were prepared in petri dishes. six wells measuring 4 mm in diameter we ... | 2005 | 16268399 |
| cell adsorption and selective desorption for separation of microbial cells by using chitosan-immobilized silica. | cell adsorption and selective desorption for separation of microbial cells were conducted by using chitosan-immobilized silica (cis). when chitosan was immobilized onto silica surfaces with glutaraldehyde, bacterial cells adsorbed well and retained viability. testing of the adsorption and desorption ability of cis using various microbes such as escherichia coli, aeromonas hydrophila, pseudomonas aeruginosa, bacillus subtilis, micrococcus luteus, staphylococcus aureus, staphylococcus epidermidis, ... | 2005 | 16332887 |
| cariogenic virulence characteristics of mutans streptococci isolated from caries-active and caries-free adults. | the objective of the study was to compare aciduricity (ability, to live in acid), acidogenicity (ability to produce acid), and intracellular polysaccharide production of mutans streptococci (ms) strains isolated from caries-active (ca, with one or more cavitated lesions) and caries-free (cf, with no clinically observable new caries in the last five years) adults. forty-three ms strains from 17 of 17 ca adults, and 14 strains from eight of 12 cf adults were investigated. ms isolates' growth, surv ... | 2005 | 16454241 |
| dental caries: an infectious and transmissible disease. | by definition, dental caries is an infectious and transmissible disease because it is caused by bacteria colonizing the tooth surfaces. unlike most infectious diseases affecting humans, caries is the result of an imbalance of the indigenous oral biota rather than a nonindigenous, exogenous pathogen. the introduction of refined sugar into modern society's diet has tipped the balance from health to disease. new insight into the natural history of the leading cariogenic bacteria, the mutans strepto ... | 2005 | 17036539 |
| influence of dentin on the effectiveness of antibacterial agents. | the influence of dentin on the effectiveness of three antibacterial agents (triclosan, glutaraldehyde, naocl) on streptococcus mutans, s. sobrinus, and lactobacillus acidophilus was tested using the agar diffusion method with and without bovine dentin discs (200 mum and 500 mum thickness) placed between bacteria and test substances. the effect of 0.3% triclosan on all tester strains (100%) was reduced after passage through 500 mum dentin discs to 0% (l. acidophilus) and to 22% and 28% (s. mutans ... | 2005 | 15671825 |
| cariogenic bacteria and caries risk in elderly japanese aged 80 and older with at least 20 teeth. | to assess caries risk and check the presence of four commonly found oral cariogenic bacteria in "8020" achievers. | 2006 | 17038077 |
| disease prevention: caries risk assessment. | 2006 | 17058393 | |
| influence of a fluoride mouthrinse on mutans streptococci in schoolchildren. | this study aimed to determine whether the long-term use of a fluoride mouthrinse affects the salivary levels of mutans streptococci. two hundred and fifteen schoolchildren (aged 9-10 years) participated. one hundred and forty-nine of these children had used a fluoride mouthrinse since 5 years of age at nursery school, and the remaining 66 children had not. dft (decayed and filled teeth) was recorded, and the salivary levels of streptococcus mutans and streptococcus sobrinus were measured using m ... | 2006 | 17063021 |
| photosensitization of in vitro biofilms by toluidine blue o combined with a light-emitting diode. | in natural ecosystems, micro-organisms grow preferentially attached to surfaces, forming matrix-enclosed biofilms. the aim of this study was to determine photodestruction levels in biofilms after subjecting them to photodynamic therapy. biofilms of streptococcus mutans, s. sobrinus, and s. sanguinis were grown on enamel slabs for 3, 5 or 7 d. both the number of viable micro-organisms and the concentration of water-insoluble polysaccharides were analysed, and mineral loss (deltaz) analyses were p ... | 2006 | 16460343 |
| in vitro antibacterial activity of the peptide psvp-10 against streptococcus mutans and streptococcus sobrinus with and without glycocalyx. | the antibacterial activity of the peptide psvp-10 obtained from pseudomonas sp. r10 against streptococcus mutans and streptococcus sobrinus was investigated. one hundred and twenty strains of s. mutans and 120 strains of s. sobrinus with and without glycocalyx were isolated from saliva samples in trypticase-yeast-cysteine-sucrose-bacitracin (tycsb) agar. bacterial identification was made by polymerase chain reaction. glycocalyx production was observed in modified tycsb agar and confirmed with a ... | 2006 | 16472991 |
| anticariogenic activity of macelignan isolated from myristica fragrans (nutmeg) against streptococcus mutans. | the occurrence of dental caries is mainly associated with oral pathogens, especially cariogenic streptococcus mutans. preliminary antibacterial screening revealed that the extract of myristica fragrans, widely cultivated for the spice and flavor of foods, possessed strong inhibitory activity against s. mutans. the anticariogenic compound was successfully isolated from the methanol extract of m. fragrans by repeated silica gel chromatography, and its structure was identified as macelignan by inst ... | 2006 | 16492529 |
| black teeth: beauty or caries prevention? practice and beliefs of the kammu people. | to be beautiful and caries-free, kammu women in laos and vietnam habitually paint their teeth black. although this practice existed for many generations, it is now known only among the elderly. | 2006 | 16515671 |
| preparation and antimicrobial activity of gelatin microparticles containing propolis against oral pathogens. | gelatin microparticles containing propolis ethanolic extractive solution were prepared by spray-drying technique. particles with regular morphology, mean diameter ranging of 2.27 microm to 2.48 microm, and good entrapment efficiency for propolis were obtained. the in vitro antimicrobial activity of microparticles was evaluated against microorganisms of oral importance (enterococcus faecalis, streptococcus salivarius, streptococcus sanguinis, streptococcus mitis, streptococcus mutans, streptococc ... | 2006 | 16537203 |
| in vitro binding interactions of oral bacteria with immobilized fructosyltransferase. | the objective of the present study was to explore the role of immobilized fructosyltransferase (ftf) in adhesion process. | 2006 | 16553744 |
| [a study of the physicochemical and biological properties of mutanase from trichoderma harzianum]. | to determine the physicochemical properties of the mutanase of trichoderma harzianum isolated from china and to study the influence of mutanase on the adherence of oral streptococci and the structure of oral biofilms. | 2006 | 16620625 |
| human t-cell responses to oral streptococci in human pbmc-nod/scid mice. | we investigated cellular and humoral immune responses to oral biofilm bacteria, including streptococcus mutans, streptococcus anginosus, streptococcus sobrinus, and streptococcus sanguinis, in nod/scid mice immunized with human peripheral blood mononuclear cells (hu-pbmc-nod/scid mice) to explore the pathogenicity of each of those organisms in dental and oral inflammatory diseases. hu-pbmc-nod/scid mice were immunized by intraperitoneal injections with the whole cells of the streptococci once a ... | 2006 | 16626374 |
| modeling shifts in microbial populations associated with health or disease. | stable microbial communities associated with health can be disrupted by altered environmental conditions. periodontal diseases are associated with changes in the resident oral microflora. for example, as gingivitis develops, a key change in the microbial composition of dental plaque is the ascendancy of actinomyces spp. and gram-negative rods at the expense of streptococcus spp. we describe the use of an in vitro model to replicate this population shift, first with a dual-species model (actinomy ... | 2006 | 16672517 |
| evaluation of fluoride release from teeth after topical application of naf, snf2 and apf and antimicrobial activity on mutans streptococci. | the objectives of this study were to evaluate and compare the amount and pattern of fluoride release from teeth after topical application of 2% naf, 8% snf2 and 1.23% apf at different time intervals. the growth inhibitory effects of this released fluoride ion was assessed on mutans streptococci (ms) and correlated with the fluoride release. forty premolars divided into four groups were subjected to different topical fluoride treatments. all the teeth were immersed individually in deionized water ... | 2006 | 16683673 |
| in vitro assessment of antimicrobial peptides as potential agents against several oral bacteria. | antimicrobial peptides are components of the innate immunity that play an important role in systemic and oral health. | 2006 | 16687459 |
| the effect of traditional african food mixtures on growth, ph and extracellular polysaccharide production by mutans streptococci in vitro. | four, traditional african food mixtures (maize plus milk and sugar, maize plus gravy, samp plus beans, brown bread plus margarine and peanut butter) were evaluated for their ability to sustain the growth of mutans streptococci in batch culture. a synthetic complex medium, brain heart infusion with 3% sucrose was used as an experimental control. six nctc laboratory reference strains and five clinical isolates collected from the plaque of children were investigated. the doubling time of bacterial ... | 2006 | 16701622 |
| mutans streptococci: acquisition and transmission. | dental caries is an infectious and transmissible disease. the mutans streptococci (ms) are infectious agents most strongly associated with dental caries. earlier studies demonstrated that infants acquire ms from their mothers and only after the eruption of primary teeth. more recent studies indicate that ms can colonize the mouths of predentate infants and that horizontal as well as vertical transmission does occur. the purpose of this paper was to demonstrate that these findings will likely fac ... | 2006 | 16708784 |
| xylitol, sweeteners, and dental caries. | the purpose of this report was to provide an overview of xylitol and other polyol sweeteners and dental caries for clinicians and to discuss current applications for dental practice and potential community-based public health interventions. xylitol, like other polyol sweeteners, is a naturally occurring sugar alcohol. studies suggest polyols are noncariogenic. furthermore, studies indicate that xylitol can decrease mutans streptococci levels in plaque and saliva and can reduce dental caries in y ... | 2006 | 16708791 |
| in vitro anti-cariogenic activity of dichloromethane fraction from rheum undulatum l. root. | this study aimed to evaluate in vitro effects of rheum undulatum l. root on the development of dental caries, especially its effects on viability, dental plaque formation, and glycolytic acid production of streptococcus mutans and streptococcus sobrinus. methanol extract of rheum undulatum l. root and its fractions were prepared and tested. among the test extract and fractions, dichloromethane fraction (df) showed the most active antibacterial activity (inhibition zone: 13-17 mm) against s. muta ... | 2006 | 16833017 |
| presence of mutans streptococci and candida spp. in dental plaque/dentine of carious teeth and early childhood caries. | this study determined the presence of mutans streptococci and candida spp. in supragingival dental plaque and infected dentine of caries-free children, with early childhood caries and caries. pooled samples of dental plaque and infected dentine were collected from 56 children aged 1-5 years, which were divided into 3 groups: early childhood caries (ecc); caries and caries-free. infected dentine was collected in ecc and caries groups to compare the frequency of these microorganisms in the collect ... | 2006 | 16890907 |
| in vitro inhibitory effects of polygonum cuspidatum on bacterial viability and virulence factors of streptococcus mutans and streptococcus sobrinus. | polygonum cuspidatum has been used in korean folk medicine to improve oral hygiene. this study was performed to evaluate the effects of methanol extract from root of p. cuspidatum (mep) on bacterial viability and the virulence factors of streptococcus mutans and streptococcus sobrinus. | 2006 | 16914113 |
| bacterial profile in primary teeth with necrotic pulp and periapical lesions. | the objective of this study was to evaluate the bacterial profile in root canals of human primary teeth with necrotic pulp and periapical lesions using bacterial culture. a total of 20 primary teeth with necrotic pulp and radiographically visible radiolucent areas in the region of the bone furcation and/or the periapical region were selected. after crown access, 4 sterile absorbent paper points were introduced sequentially into the root canal for collection of material. after 30 s, the paper poi ... | 2006 | 16924343 |
| the in vitro effect of fluoridated milk in a bacterial biofilm--enamel model. | the purpose of this study was to investigate the effect of milk and fluoridated milk on bacterially induced caries-like lesions. | 2006 | 16936902 |
| influence of biosurfactant on interactive forces between mutans streptococci and enamel measured by atomic force microscopy. | although interactive forces, influenced by environmental conditions, between oral bacteria and tooth surfaces are important for the development of plaque, they have never been estimated. it is hypothesized that interactive forces, as measured by atomic force microscopy, between enamel with or without a pellicle and two strains of mutans streptococci become less attractive by the application of a streptococcus mitis bms biosurfactant coating. upon approach of each of the strains toward bare and p ... | 2006 | 16373681 |
| effect of mixed mutans streptococci colonization on caries development. | to evaluate the clinical importance of mixed mutans streptococci colonization in predicting caries in preschool children. | 2006 | 16390341 |
| the influence of a novel propolis on mutans streptococci biofilms and caries development in rats. | a flavonoids-free brazilian propolis (type 6) showed biological effects against mutans streptococci and inhibited the activity of glucosyltransferases. this study evaluated the influence of the ethanolic extract of a novel type of propolis (eep) and its purified hexane fraction (eeh) on mutans streptococci biofilms and the development of dental caries in rats. the chemical composition of the propolis extracts were examined by gas chromatography/mass spectrometry. the effects of eep and eeh on st ... | 2006 | 16054589 |
| quantitative determination of adhesion patterns of cariogenic streptococci to various orthodontic adhesives. | to investigate the adhesion of various cariogenic streptococci to orthodontic adhesives. | 2006 | 17029524 |
| evaluation of the contamination and disinfection methods of toothbrushes used by 24- to 48-month-old children. | the purpose of this study was to evaluate: (1) in vivo the contamination by mutans streptococci (ms) of toothbrushes after use on 52 children (24-48 months old) by a single dentist; (2) in vivo the efficacy of 3 solutions (periogard, brushtox, and a cosmocil cqa and myacide pharma bpa-based experimental solution) in the disinfection of these toothbrushes through a randomized clinical trial; and (3) in vitro the antimicrobial activity of the solutions by the agar diffusion test using 15 microbial ... | 2006 | 17367032 |
| streptococcus sobrinus in children and its influence on caries activity. | this was to study the longitudinal assessment of caries activity of streptococcus sobrinus (ss) positive children during their mixed dentition. | 2006 | 17140523 |
| evaluation of cariogenic bacteria. | the evaluation of mutans streptococci (ms) is one of the index for caries risk. dentocult(tm) and crt(tm) are commercial kits to detect and evaluate ms, conveniently. however, the evaluation of ms has also been carried out simply using an instruction manual. but the instruction manual is not easy to use for evaluation of ms. the aim of this study was to examine the utility of modified mitis-salivalius bacitracin (msb) agar medium compared with msb agar medium and commercial kits, and to establis ... | 2007 | 19212495 |
| [study on exopolysaccharides produced by planktonic and attached streptococcus sobrinus]. | to elucidate the amount of exopolysaccharides produced by streptococcus sobrinus when cultivated at different conditions. | 2007 | 17377707 |
| evaluation of enhanced hygroscopicity, bifidogenicity, and anticariogenicity of enzymatically synthesized beta-galactosyl-trehalose oligosaccharides. | beta-galactosyl-trehalose oligosaccharides (beta-gtos) were enzymatically prepared as a mixture of 6-beta-galactosyl-trehalose (1) and 4-beta-galactosyl-trehalose (2) with a 9:1 ratio (w/w). the beta-gto mixture showed a highly enhanced hygroscopicity as compared to those of trehalose and other sugars used. at 72 h of incubation under 90% relative humidity and room temperature, it had a large increase in weight due to its moisture absorption, which was five times larger than that of trehalose, 1 ... | 2007 | 17429983 |
| immunoassay-based diagnostic point-of-care technology for oral specimen. | we have outlined our progress in developing a novel point-of-care platform to quantify micro-organisms causing dental infections and/or inflammatory markers reflecting an oral disease status. this system is based on a sandwich immunoassay technology known as abicap (antibody immuno column for analytical processes) using poly-horseradish peroxidase conjugates. this assay enabled us to quantify 500 colony-forming units of streptococcus sobrinus per milliliter of saliva. the platform allows rapid a ... | 2007 | 17435155 |
| protection offered by root-surface restorative materials against biofilm challenge. | the prevalence of root-surface caries is increasing. we hypothesized that some restorative materials are protective against cariogenic challenge on root surfaces. our goal was to study the effects of different restorative materials on root surfaces incubated with an oral biofilm generated in an artificial mouth. a biofilm of streptococcus mutans, streptococcus sobrinus, lactobacillus rhamnosus, and actinomyces naeslundii was co-cultured for 21 days on 24 glass-ionomer cement, resin-modified glas ... | 2007 | 17452563 |
| antibacterial activity and shear bond strength of 4-methacryloxyethyl trimellitate anhydride/methyl methacrylate-tri-n-butyl borane resin containing an antibacterial agent. | to produce an antibacterial adhesive for orthodontic bonding without compromising the mechanical property. | 2007 | 17465666 |
| identification of periodontopathic bacteria in gingival tissue of japanese periodontitis patients. | the identification of invading periodontopathic bacteria in tissues is important to determine their role in the pathogenesis of periodontal disease. the objective of this study was to identify periodontopathic bacteria in diseased gingival tissue of periodontitis patients. | 2007 | 17488447 |
| antibacterial activity of four glass ionomer cements used in atraumatic restorative treatment. | the in vitro antibacterial activity of four glass ionomer cements (fuji ix, ketac molar, vidrion r and vitromolar) indicated for atraumatic restorative treatment (art) was studied against strains of bacteria involved in the development of oral diseases, streptococcus mutans, streptococcus sobrinus, lactobacillus acidophilus and actinomyces viscosus. the agar plate diffusion test was used for the cultures, which included chlorhexidine as a positive control. the results demonstrated that all the c ... | 2007 | 17497079 |
| periodontal bacterial dna suppresses the immune response to mutans streptococcal glucosyltransferase. | certain cpg motifs found in bacterial dna enhance immune responses through toll-like receptor 9 (tlr-9) and may also demonstrate adjuvant properties. our objective was to determine if dna from bacteria associated with periodontal disease could affect the immune response to other bacterial antigens in the oral cavity. streptococcus sobrinus glucosyltransferase (gtf), an enzyme involved in dental caries pathogenesis, was used as a test antigen. rowett rats were injected with aluminum hydroxide (al ... | 2007 | 17517867 |
| development of species-specific primers for detection of streptococcus mutans in mixed bacterial samples. | streptococcus mutans is the major microbial pathogen associated with dental caries in children. the objectives of this study were to design and evaluate species-specific primers for the identification of s. mutans. validation of the best primer set, sm479f/r, was performed using seven s. mutans reference strains, 48 atcc non-s. mutans strains, 92 s. mutans clinical isolates, dna samples of s. mutans-streptococcus sobrinus or s. mutans-streptococcus sanguinis, and mixed bacterial dna of saliva sa ... | 2007 | 17521362 |
| in vitro effects of a fraction separated from polygonum cuspidatum root on the viability, in suspension and biofilms, and biofilm formation of mutans streptococci. | polygonum cuspidatum (polygonaceae) has traditionally been used in folk medicine to control oral diseases. nevertheless, there are no reports related to its possible effect on the diseases, particularly on biofilm-related diseases such as dental caries. in this study, we evaluated in vitro effects of a fraction separated from polygonum cuspidatum root on the viability, in both suspension and biofilms, and the biofilm formation of mutans streptococci. the separated fraction (f1) showed bacteriost ... | 2007 | 17543483 |
| a novel tem contrasting technique for extracellular polysaccharides in in vitro biofilms. | in the past, the visualization of the extracellular matrix of biofilms on an ultrastructural level was hampered by shrinkage artifacts. in addition, the reproducible contrasting of extracellular polysaccharides (eps) has not satisfactorily been solved. here we describe a method overcoming these difficulties, which produces artifact-free transmission electron microscopic (tem) images using multispecies biofilms grown in vitro. sufficient contrast was achieved by replacing schiff's reagent with th ... | 2007 | 17557287 |
| prevalence of cariogenic streptococci on incisor brackets detected by polymerase chain reaction. | streptococcus mutans and streptococcus sobrinus are the main causative organisms for enamel demineralization during orthodontic treatment. in this study, we analyzed the prevalence of cariogenic streptococci adhering to incisor brackets. | 2007 | 17561051 |
| thermodynamics of the binding of the c-terminal repeat domain of streptococcus sobrinus glucosyltransferase-i to dextran. | glucosyltransferases (gtfs) secreted by mutans streptococci and some other lactic acid bacteria catalyze glucan synthesis from sucrose, and possess a c-terminal glucan-binding domain (gbd) containing homologous, directly repeating units. we prepared a series of c-terminal truncated forms of the gbd of streptococcus sobrinus gtf-i and studied their binding to dextran by isothermal titration calorimetry. the binding of all truncates was strongly exothermic. their titration curves were analyzed ass ... | 2007 | 17580962 |
| one of two gbpc gene homologues is involved in dextran-dependent aggregation of streptococcus sobrinus. | streptococcus sobrinus exhibits marked dextran-dependent aggregation mediated by glucan-binding proteins (gbps). in contrast to streptococcus mutans, in which the gbpc gene responsible for dextran-dependent aggregation of this organism has been characterized, genes encoding the s. sobrinus gbps have not yet been identified. | 2007 | 17600535 |
| isolation and characterization of the mutans streptococci from the dental plaques in koreans. | mutans streptococci have been implicated as cariogenic bacteria in dental caries because they can produce high levels of dental caries-causing lactic acid and extracellular polysaccharide. the aim of this study was to isolate and characterize the mutans streptococci from the dental plaque obtained from koreans. the dental plaque samples were collected from the anterior and molar teeth of both jaws in 155 subjects (aged 2 to 33.2 years, average age 13.7+/-4.7 years). the samples were diluted by 1 ... | 2007 | 17618231 |
| in vitro antimicrobial activity of peroxide-based bleaching agents. | antibacterial activity of 4 commercial bleaching agents (day white, colgate platinum, whiteness 10% and 16%) on 6 oral pathogens (streptococcus mutans, streptococcus sobrinus, streptococcus sanguinis, candida albicans, lactobacillus casei, and lactobacillus acidophilus) and staphylococcus aureus were evaluated. a chlorhexidine solution was used as a positive control, while distilled water was the negative control. bleaching agents and control materials were inserted in sterilized stainless-steel ... | 2007 | 17625621 |
| inhibition of bacterial adherence to saliva-coated through plant lectins. | in the present study, we evaluated the ability of lectin from talisia esculenta (tel) and a protein from labramia bojeri seeds (labramin) to inhibit adherence of microorganisms and exert antimicrobial effects. the minimum inhibitory and bactericidal concentrations of these proteins were determined using 5 species of bacteria: streptococcus mutans ua159, streptococcus sobrinus 6715, streptococcus sanguinis atcc10556, streptococcus mitis atcc903 and streptococcus oralis pb182. in addition, an adhe ... | 2007 | 17634727 |
| targeted profiling of oral bacteria in human saliva and in vitro biofilms with quantitative real-time pcr. | an in vitro plaque model based on the use of human salivary bacteria and tooth-like surfaces was previously developed for studying the formation of oral biofilm and its use for pre-clinical testing of candidate antimicrobial or antiplaque agents. in this study, a quantitative taqman pcr assay (qpcr) was developed to compare the bacterial compositions of in vitro biofilms to parent saliva samples, and to determine the relative contributions of different species in the formation of the oral biofil ... | 2007 | 17653931 |
| microbiota of exposed root surfaces after fluoride, chlorhexidine, and periodontal maintenance therapy: a 3-year evaluation. | fluoride and chlorhexidine (chx) are state-of-the-art preventive measures for remineralizing teeth and for preventing plaque accumulation. the aim of this study was to examine the effects of fluoride and chx varnishes on root caries and microbiota located on root surfaces. | 2007 | 17668978 |
| [arbitrarily primed-pcr detection of streptococcus mutans and streptococcus sobrinus in dental plaque of children with high dmft and no caries]. | to isolate and identify streptococcus mutans (sm) and streptococcus sobrinus (ss) in dental plaque of children with high dmft and no caries by selective medium, biochemical methods and arbitrarily primed-polymerase chain reaction (ap-pcr). | 2007 | 17683669 |
| factors influencing oral colonization of mutans streptococci in young children. | this paper aims to critically review current knowledge about the key factors involved in oral colonization of the cariogenic group of bacteria, mutans streptococci (ms) in young children. ms, consisting mainly of the species streptococcus mutans and streptococcus sobrinus, are commonly cultured from the mouths of infants, with prevalence of infection ranging from around 30 per cent in 3 month old predentate children to over 80 per cent in 24 month old children with primary teeth. ms is usually t ... | 2007 | 17687953 |
| in vitro inhibition of bacterial growth using different dental adhesive systems. | this study evaluated the antibacterial potential of four different adhesive systems. | 2007 | 17695612 |
| caries risk assessment in bosnian children using cariogram computer model. | to examine caries risk using the cariogram model, interactive pc program for caries risk evaluation in 12-year-old children and to correlate caries risk in children of different socioeconomic backgrounds. | 2007 | 17695739 |
| lactobacillus-mediated interference of mutans streptococci in caries-free vs. caries-active subjects. | in order to assess whether naturally occurring oral lactobacilli have probiotic properties, lactobacilli were isolated from saliva and plaque from children and adolescents, with or without caries lesions. the interference capacities of these lactobacilli were investigated against a panel of 13 clinical isolates and reference strains of streptococcus mutans and streptococcus sobrinus, as well as against the subject's autologous mutans streptococci, using the agar-overlay technique. lactobacillus- ... | 2007 | 17697171 |
| [microbiologic and molecular diagnostic of cariogenic bacteria in pregnant women from the araucania region of chile]. | dental caries is a transmissible infectious disease in which streptococcus mutans is a principal protagonist. although it is widely believed that pregnancy is harmful to teeth, the effect of pregnancy on the development of caries is not clear. considering this situation, the aim of the present study was to evaluate the levels of infection and to differentiate bacterial species with cariogenic potential in pregnant women from the araucania region in chile, by bacteriological and molecular analysi ... | 2007 | 17728913 |
| in vitro inhibition of oral streptococci binding to the acquired pellicle by algal lectins. | the initial colonization of the tooth by streptococci involves their attachment to adsorbed components of the acquired pellicle. avoiding this adhesion may be successful in preventing caries at early stages. salivary mucins are glycoproteins that when absorbed onto hydroxyapatite may provide binding sites for certain bacteria. algal lectins may be especially interesting for oral antiadhesion trials because of their great stability and high specificity for mucins. this work aimed to evaluate the ... | 2007 | 17897204 |
| chitosan-containing gum chewing accelerates antibacterial effect with an increase in salivary secretion. | this study was designed to confirm the mechanical efficacy of chewing chitosan-containing gum to suppress the growth of oral bacteria compared to a mouth rinse, and to demonstrate the increased salivary secretion due to chewing chitosan-containing gum. | 2007 | 17900783 |
| phenolic compounds from sugarcane molasses possessing antibacterial activity against cariogenic bacteria. | during the course of our research into the use of cane by-products from sugar manufacturing, we have studied the isolation and structural determination of bioactive compounds present in sugarcane molasses. in this study, dehydrodiconiferylalcohol-9'-o-beta-d-glucopyranoside (1) and isoorientin-7, 3'-o-dimethyl ether (2) were isolated as antibacterial active compounds against cariogenic bacteria. their structures were elucidated by (1)h-nmr, (13)c-nmr and esi-ms. the activities of these isolated ... | 2007 | 17938552 |
| rapid detection of the cariogenic pathogens streptococcus mutans and streptococcus sobrinus using loop-mediated isothermal amplification. | streptococcus mutans and streptococcus sobrinus are associated with the development of dental caries in humans. in this study, we developed a rapid, sensitive method for detecting these major cariogenic pathogens using loop-mediated isothermal amplification (lamp). the assay procedure is quite simple: the amplification is carried out in a single tube under isothermal conditions at 63 degrees c, and the result can be obtained in less than 1 h. | 2007 | 17949337 |
| manganese affects streptococcus mutans virulence gene expression. | studies of trace metals in drinking water and tooth enamel have suggested a caries-promoting potential for manganese (mn). additionally, mn has been shown to be essential for the expression of mutans streptococci virulence factors such as the glucan-binding lectin (gbl) of streptococcus sobrinus. the streptococcus mutans glucan-binding protein (gbp) gbpc is the functional analogue of the s. sobrinus gbl. s. mutans gbps have been shown to contribute to biofilm architecture and virulence. this stu ... | 2007 | 17992013 |
| comparative study of the antimicrobial efficacy of chlorhexidine gel, chlorhexidine solution and sodium hypochlorite as endodontic irrigants. | the purpose of this study was to assess the in vitro the antimicrobial efficacy of chlorhexidine gluconate gel as an endodontic auxiliary chemical substance compared to sodium hypochlorite (naocl) and chlorhexidine gluconate solution. the antimicrobial efficacy of the tested substances was evaluated using the agar diffusion test. the growth inhibition zones produced by 0.2%, 1% and 2% chlorhexidine gel were evaluated against 5 facultative anaerobic bacteria and 4 pigmented gram-negative anaerobe ... | 2007 | 18278298 |
| detection of streptococcus mutans and streptococcus sobrinus in dental plaque samples from brazilian preschool children by polymerase chain reaction. | the purposes of this study were to detect s. mutans and s. sobrinus by polymerase chain reaction (pcr) amplification, and to relate their presence to the incidence of dental caries in 42 brazilian preschool children. dental plaque samples were collected from the cervical margin of all erupted teeth of 5-6 years old children with primary dentition, using a sterile explorer. examination of the dmft (decayed, missing, filled teeth) index, performed following the world health organization (who) cari ... | 2007 | 18278304 |
| quantitative determination of adhesion patterns of cariogenic streptococci to various orthodontic brackets. | information about the adhesion of cariogenic streptococci to orthodontic brackets can help to determine a means for preventing enamel demineralization. the purpose of this study was to investigate the adhesion levels of 4 cariogenic streptococci strains to various orthodontic brackets with respect to bracket type, bacterial strain, incubation time, and saliva coating. | 2007 | 18068602 |
| antibacterial activity of triterpene acids and semi-synthetic derivatives against oral pathogens. | triterpene acids (ursolic, oleanoic, gypsogenic, and sumaresinolic acids) isolated from miconia species, along with a mixture of ursolic and oleanolic acids and a mixture of maslinic and 2-a-hydroxyursolic acids, as well as ursolic acid derivatives were evaluated against the following microorganisms: streptococcus mutans, streptococcus mitis, streptococcus sanguinis, streptococcus salivarius, streptococcus sobrinus, and enterococcus faecalis, which are potentially responsible for the formation o ... | 2007 | 18069238 |
| salivary mutans streptococci counts as indicators in caries risk assessment in 6-7-year-old chinese children. | this study aimed to determine whether salivary mutans streptococci (ms) counts in chinese children had any value in the prediction of new caries in the permanent dentition in the age interval of 6.5-8.5 years. | 2007 | 16949192 |
| immunogenic and protective potential of mutans streptococcal glucosyltransferase peptide constructs selected by major histocompatibility complex class ii allele binding. | mutans streptococcal glucosyltransferases (gtf) have been demonstrated to be effective components of dental caries vaccines. we had previously selected peptide subunits of gtf for vaccine development based on putative functional significance and conservation of gtf primary structure among enzyme isoforms. in this study, 20 20-mer linear gtf peptides were synthesized, 17 identified on the basis of the highest potential major histocompatibility complex (mhc) class ii-binding activity using compute ... | 2007 | 17088351 |
| protective efficacy of a targeted anti-caries dna plasmid against cariogenic bacteria infections. | we have previously reported that a targeted anti-caries dna plasmid pgja-p/vax which was constructed against the antigenic determinants of streptococcus mutans (s. mutans) successfully induced antibody responses in mice and monkeys. the present study explored the protective efficacy of pgja-p/vax against cariogenic bacterial challenge. groups of rats were orally challenged with s. mutans or streptococcus sobrinus (s. sobrinus) and then immunized with pgja-p/vax or the vector pvax1 intranasally. ... | 2007 | 17095128 |
| efficacy of gasiform ozone and photodynamic therapy on a multispecies oral biofilm in vitro. | ozone gas and photodynamic therapy (pdt) have been claimed to be antimicrobially effective. this study assessed their antimicrobial potential in vitro. mature six-species oral biofilms were treated as follows (n = 9 per group): (i) a 60-s application of gasiform vacuum-ozone or vacuum alone (on wet or air-dried biofilm samples); (ii) pdt (i.e. methylene blue in combination with or without a diode soft laser, and a soft laser alone); or (iii) antimicrobial solutions: immersion of biofilms for 60 ... | 2007 | 17305720 |
| inflammatory immune responses by water-insoluble alpha-glucans. | water-insoluble alpha-glucans are synthesized from sucrose by glucosyltransferase-i of mutans streptococci and play an important role in the development of dental plaque. several types of beta-glucans in fungal cell wall components and water-soluble alpha-glucans from streptococcus mutans are known to modulate innate immunity. in the present study, we investigated whether water-insoluble alpha-glucans also induced inflammatory innate immune responses. our results showed that water-insoluble alph ... | 2007 | 17314256 |