Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| genetic basis of coaggregation receptor polysaccharide biosynthesis in streptococcus sanguinis and related species. | interbacterial adhesion between streptococci and actinomyces promotes early dental plaque biofilm development. recognition of coaggregation receptor polysaccharides (rps) on strains of streptococcus sanguinis, streptococcus gordonii and streptococcus oralis by actinomyces spp. type 2 fimbriae is the principal mechanism of these interactions. previous studies of genetic loci for synthesis of rps (rps) and rps precursors (rml, gale1 and gale2) in s. gordonii 38 and s. oralis 34 revealed difference ... | 2014 | 24397790 |
| isolation of streptococcus tigurinus - a novel member of streptococcus mitis group from a case of periodontitis. | streptococcus tigurinus is a new member of the streptococcus viridians group and is closely related to streptococcus mitis, streptococcus pneumoniae, streptococcus pseudopneumoniae, streptococcus oralis, and streptococcus infantis. the type strain az_3a(t) of s. tigurinus was originally isolated from a patient with infective endocarditis. accurate identification of s. tigurinus is facilitated only by newer molecular methods like 16s rrna gene analysis. during the course of study on bacteraemia a ... | 2014 | 24974898 |
| effect of enamel morphology on nanoscale adhesion forces of streptococcal bacteria : an afm study. | we explore the influence of enamel surface morphology on nanoscale bacterial adhesion forces. three dimensional morphology characteristics of enamel slices, which were treated with phosphoric acid (for 0 s, 5 s, 10 s, 20 s, and 30 s), were acquired. adhesion forces of three initial colonizers (streptococcus oralis, streptococcus sanguinis, and streptococcus mitis) and two cariogenic bacterial strains (streptococcus mutans and streptococcus sobrinus) with etched enamel surfaces were determined. c ... | 2015 | 26482011 |
| clinical significance and characterization of streptococcus tigurinus isolates in an adult population. | streptococcus tigurinus is a newly described member of the streptococcus mitis group. due to the difficulty in distinguishing viridans group streptococci (vgs) by phenotype, analysis of 16s rrna sequences is necessary for the accurate identification of most species. through a laboratory policy of analyzing all clinically significant isolates from the vgs group by16s rrna gene sequencing, we identified 14 s. tigurinus isolates from 11 patients. the vitek 2 system most commonly gave an excellent r ... | 2015 | 26354809 |
| antibiotic therapies in maxillofacial surgery in the context of prophylaxis. | there is no single pattern for preventive action as to the duration and type of antibiotic therapy in maxillofacial surgery. in these circumstances, it appears reasonable to set relevant standards for prophylactic procedures after such surgeries. | 2015 | 25710028 |
| identification of proteins in streptococcus pneumoniae by reverse vaccinology and genetic diversity of these proteins in clinical isolates. | streptococcus pneumoniae is a major cause of morbidity and mortality worldwide. virulence-associated proteins common and conserved among all capsular types now represent the best strategy to combat pneumococcal infections. our aim was to identify conserved targets in pneumococci that showed positive prediction for lipoprotein and extracellular subcellular location using bioinformatics programs and verify the distribution and the degree of conservation of these targets in pneumococci. these targe ... | 2015 | 25448632 |
| neuraminidase-producing oral mitis group streptococci potentially contribute to influenza viral infection and reduction in antiviral efficacy of zanamivir. | influenza is a serious respiratory disease among immunocompromised individuals, such as the elderly, and its prevention is an urgent social issue. influenza viruses rely on neuraminidase (na) activity to release progeny viruses from infected cells and spreading the infection. na is, therefore, an important target of anti-influenza drugs. a causal relationship between bacteria and influenza virus infection has not yet been established, however, a positive correlation between them has been reporte ... | 2015 | 25001578 |
| microbiocenosis in inflammatory processes of marginal gum among children. | the development of gingivitis among children remains actual problem in modern dentistry due to high prevalence, disorders of dental-alveolar functions and development of parodontium diseases at the older age. the microorganisms occupying dental chamfer play the leading role in parodontium tissues damage. | 2015 | 26887119 |
| comparison of species identification of endocarditis associated viridans streptococci using rnpb genotyping and 2 maldi-tof systems. | streptococcus spp. are important causes of infective endocarditis but challenging in species identification. this study compared identification based on sequence determination of the rnpb gene with 2 systems of matrix-assisted laser desorption ionization-time of flight mass spectrometry, maldi biotyper (bruker) and vitek ms ivd (biomérieux). blood culture isolates of viridans streptococci from 63 patients with infective endocarditis were tested. the 3 methods showed full agreement for all 36 iso ... | 2015 | 25616316 |
| environment and colonisation sequence are key parameters driving cooperation and competition between pseudomonas aeruginosa cystic fibrosis strains and oral commensal streptococci. | cystic fibrosis (cf) patient airways harbour diverse microbial consortia that, in addition to the recognized principal pathogen pseudomonas aeruginosa, include other bacteria commonly regarded as commensals. the latter include the oral (viridans) streptococci, which recent evidence indicates play an active role during infection of this environmentally diverse niche. as the interactions between inhabitants of the cf airway can potentially alter disease progression, it is important to identify key ... | 2015 | 25710466 |
| flavonoid glucosylation by non-leloir glycosyltransferases: formation of multiple derivatives of 3,5,7,3',4'-pentahydroxyflavane stereoisomers. | flavonoids are known to possess a multitude of biological activities. therefore, diversification of the core structures is of considerable interest. one of nature's important tailoring reactions in the generation of bioactive compounds is glycosylation, which is able to influence numerous molecular properties. here, we examined two non-leloir glycosyltransferases that use sucrose as an inexpensive carbohydrate donor, glycosyltransferase r from streptococcus oralis (gtfr) and amylosucrase from ne ... | 2015 | 26124069 |
| bacteria-induced intracellular signalling in platelets. | multiple studies have now shown that various species of bacteria can stimulate platelets; many in a strain and donor-dependent manner. the signalling pathways underlying this platelet activation has been the subject of scrutiny for the last decade. the best-delineated pathway is that in response to streptococcal species, such as streptococcus sanguinis (s. sanguinis), streptococcus gordonii (s. gordonii) and streptococcus oralis (s. oralis), where a pathway is initiated by the engagement of the ... | 2015 | 25807064 |
| proteomic and transcriptional analysis of interaction between oral microbiota porphyromonas gingivalis and streptococcus oralis. | porphyromonas gingivalis, a major periodontal pathogen, forms biofilm with other oral bacteria such as streptococci. here, by using shotgun proteomics, we examined the molecular basis of mixed-biofilm formation by p. gingivalis with streptococcus oralis. we identified a total of 593 bacterial proteins in the biofilm. compared to the expression profile in the p. gingivalis monobiofilm, the expression of three proteins was induced and that of 31 proteins was suppressed in the mixed biofilm. additi ... | 2015 | 25341202 |
| the effect of a streptococci containing probiotic in periodontal therapy: a randomized controlled trial. | to evaluate the adjunctive effects of a streptococcus oralis kj3, streptococcus uberis kj2 and streptococcus rattus jh145 containing probiotic tablet after scaling and root planing (srp). | 2015 | 26427036 |
| candida-streptococcal mucosal biofilms display distinct structural and virulence characteristics depending on growth conditions and hyphal morphotypes. | candida albicans and streptococci of the mitis group form communities in multiple oral sites, where moisture and nutrient availability can change spatially or temporally. this study evaluated structural and virulence characteristics of candida-streptococcal biofilms formed on moist or semidry mucosal surfaces, and tested the effects of nutrient availability and hyphal morphotype on dual-species biofilms. three-dimensional models of the oral mucosa formed by immortalized keratinocytes on a fibrob ... | 2015 | 25754666 |
| clinical and microbiologic evaluation of scaling and root planing per quadrant and one-stage full-mouth disinfection associated with azithromycin or chlorhexidine: a clinical randomized controlled trial. | conflicting data about the protocol of choice for non-surgical periodontal therapy with adjuvant use are still reported. this study aims to evaluate, through clinical and microbiologic parameters, the systemic use of azithromycin (az) and chlorhexidine (chx) as adjuvants to non-surgical periodontal treatment performed by one-stage full-mouth disinfection (fmd) within 24 hours or conventional quadrant scaling (qs) in four weekly sections. | 2015 | 26252751 |
| antimicrobial activity of different filling pastes for deciduous tooth treatment. | guedes-pinto paste is the filling material most employed in brazil for endodontic treatment of deciduous teeth; however, the rifocort® ointment has been removed. thus, the aim of this study was to investigate the antimicrobial potential of filling pastes, by proposing three new pharmacological associations to replace rifocort® ointment with drugs of already established antimicrobial power: nebacetin® ointment, 2% chlorhexidine gluconate gel, and maxitrol® ointment. a paste composed of iodoform, ... | 2015 | 25466327 |
| stability of saliva microbiota during moderate consumption of red wine. | this study has evaluated the effect of regular and moderate red wine consumption on the diversity and occurrence of different groups of bacteria that are representative in human saliva. | 2015 | 26433355 |
| isolation of alkaline-tolerant bacteria from primary infected root canals. | alkaline-tolerant bacteria in primary infected root canals could have enhanced survival capacity against antimicrobials commonly used in root canal treatment. the aims of this study were to isolate and characterize alkaline-tolerant bacteria before endodontic treatment (s1), after chemomechanical root canal preparation (s2), and after calcium hydroxide dressing (s3). | 2015 | 25638530 |
| a disulfide bond-forming machine is linked to the sortase-mediated pilus assembly pathway in the gram-positive bacterium actinomyces oris. | export of cell surface pilins in gram-positive bacteria likely occurs by the translocation of unfolded precursor polypeptides; however, how the unfolded pilins gain their native conformation is presently unknown. here, we present physiological studies to demonstrate that the fima pilin of actinomyces oris contains two disulfide bonds. alanine substitution of cysteine residues forming the c-terminal disulfide bridge abrogates pilus assembly, in turn eliminating biofilm formation and polymicrobial ... | 2015 | 26170452 |
| impact of acquired pellicle modification on adhesion of early colonizers. | new preventive approaches against dental erosion caused by acidic drinks and beverages include fortification of beverages with natural polymers. we have shown that the mixture of casein and mucin significantly improved the erosion-inhibiting properties of the human pellicle layer. this study aimed to investigate the effect of pellicle modification by casein, mucin and a casein-mucin mixture on the adhesion of early bacterial colonizers. test specimens of human tooth enamel were prepared, covered ... | 2015 | 26655396 |
| the assessment of oral microflora exposed to 3% ethanolic extract of brazilian green propolis preparation used for hygiene maintenance following minor oral surgeries. | the aim of this study was to investigate the influence of a topically administered hygienic preparation containing a 3% ethanolic extract of brazilian green propolis (eep-b) on oral microflora spectrum changes in a group of patients who underwent common oral surgery procedures. two gel samples were compared: the tested gel containing an active ingredient, that is, a 3% eep-b (gel ga), and a placebo as the negative control (gel gc). the collection of microbiological material included 14 patients ... | 2015 | 26380300 |
| interproximal biofilm removal by intervallic use of a sonic toothbrush compared to an oral irrigation system. | the purpose of this in-vitro study was to investigate the potential of biofilm removal in interproximal tooth regions using intervallic cleaning with an oral irrigator or a sonic toothbrush. | 2015 | 26243234 |
| removal of dental biofilms with an ultrasonically activated water stream. | acidogenic bacteria within dental plaque biofilms are the causative agents of caries. consequently, maintenance of a healthy oral environment with efficient biofilm removal strategies is important to limit caries, as well as halt progression to gingivitis and periodontitis. recently, a novel cleaning device has been described using an ultrasonically activated stream (uas) to generate a cavitation cloud of bubbles in a freely flowing water stream that has demonstrated the capacity to be effective ... | 2015 | 26056055 |
| periodontal therapy effects on nitrite related to oral bacteria: a 6-month randomized clinical trial. | nitrite is a biologic factor relevant to oral and systemic homeostasis. through an oral bacteria reduction process, it was suggested that periodontal therapy and chlorhexidine (chx) rinse could affect nitrite levels, leading to negative effects, such as an increase in blood pressure. this 6-month randomized clinical trial evaluated the effects of periodontal therapeutic protocols on salivary nitrite and its relation to subgingival bacteria. | 2015 | 25811847 |
| incorporation of staphylococci into titanium-grown biofilms: an in vitro "submucosal" biofilm model for peri-implantitis. | staphylococcus spp. are postulated to play a role in peri-implantitis. this study aimed to develop a "submucosal" in vitro biofilm model, by integrating two staphylococci into its composition. | 2015 | 26461083 |
| functional signatures of oral dysbiosis during periodontitis progression revealed by microbial metatranscriptome analysis. | periodontitis is a polymicrobial biofilm-induced inflammatory disease that affects 743 million people worldwide. the current model to explain periodontitis progression proposes that changes in the relative abundance of members of the oral microbiome lead to dysbiosis in the host-microbiome crosstalk and then to inflammation and bone loss. using combined metagenome/metatranscriptome analysis of the subgingival microbiome in progressing and non-progressing sites, we have characterized the distinct ... | 2015 | 25918553 |
| a yada-like autotransporter, hag1 in veillonella atypica is a multivalent hemagglutinin involved in adherence to oral streptococci, porphyromonas gingivalis, and human oral buccal cells. | dental biofilm development is a sequential process, and adherence between microbes and the salivary pellicle (adhesion) as well as among different microbes (co-adhesion or coaggregation) plays a critical role in building a biofilm community. the veillonella species are among the most predominant species in the oral cavity and coaggregate with many initial, early, middle, and late colonizers. similar to oral fusobacteria, they are also considered bridging species in biofilm development. however, ... | 2015 | 25440509 |
| isolated pulmonic valve endocarditis presenting as neck pain. | we discuss a unique case of a 52-year-old man with no history of intravenous drug use or dental procedures who presented with neck pain, 2 weeks of fevers, chills, night sweats, cough, and dyspnea found to have isolated pulmonic valve (pv) endocarditis. the patient did not have an associated murmur, which is commonly seen in right-sided infectious endocarditis. a transthoracic echocardiogram showed a thickened pv leaflet, with subsequent transesophageal echocardiogram showing a pv mass. speciati ... | 2015 | 26653697 |
| lipoteichoic acid of streptococcus oralis uo5: a novel biochemical structure comprising an unusual phosphorylcholine substitution pattern compared to streptococcus pneumoniae. | members of the mitis group of streptococci possess teichoic acids (tas) as integral components of their cell wall that are unique among gram-positive bacteria. both, lipoteichoic (lta) and wall teichoic acid, are formed by the same biosynthetic pathway, are of high complexity and contain phosphorylcholine (p-cho) residues. these residues serve as anchors for choline-binding proteins (cbps), some of which have been identified as virulence factors of the human pathogen streptococcus pneumoniae. we ... | 2015 | 26577602 |
| percutaneous coronary intervention for septic emboli in the left main trunk as a complication of infective endocarditis. | infective endocarditis (ie) complicated by acute myocardial infarction (ami) is frequently fatal and may require emergent interventions. however, the optimal treatment of this rare condition remains controversial as it lacks established guidelines. we successfully treated a patient with ie complicated by ami during the acute phase using percutaneous coronary intervention (pci) followed by surgery. a 73-year-old man was diagnosed with ie of the mitral and aortic valves caused by streptococcus ora ... | 2015 | 26251314 |
| transfer of penicillin resistance from streptococcus oralis to streptococcus pneumoniae identifies mure as resistance determinant. | beta-lactam resistant clinical isolates of streptococcus pneumoniae contain altered penicillin-binding protein (pbp) genes and occasionally an altered murm, presumably products of interspecies gene transfer. murm and murn are responsible for the synthesis of branched lipid ii, substrate for the pbp catalyzed transpeptidation reaction. here we used the high-level beta-lactam resistant s. oralis uo5 as donor in transformation experiments with the sensitive laboratory strain s. pneumoniae r6 as rec ... | 2015 | 26010014 |
| frosted branch angiitis in an octogenarian with infective endocarditis. | to present the difficulties in diagnosing frosted branch angiitis (fba) in an elderly person, and to present the likely immunogenic association of fba with alpha-hemolytic streptococci. | 2015 | 25383835 |
| experimental amine-epoxide sealer: a physicochemical study in comparison with ah plus and easyseal. | to compare selected physicochemical and biological properties of an experimental sealer with those of two commercially available sealers. | 2015 | 25117941 |
| streptococcus oralis cerebral abscess following monkey bite in a 2-month-old infant. | although cerebral abscesses caused by animal bites have been reported, they are extremely rare in infants and have not been described following monkey bite. a 55-day-old male infant presented with a multi-loculated streptococcus oralis cerebral abscess following a monkey bite on the scalp. there was a clinical response to antibiotic therapy and repeated surgical aspiration followed by a ventriculoperitoneal shunt. this is the first report of a patient with a brain abscess following a monkey bite ... | 2016 | 25839359 |
| streptococcus oricebi sp. nov., isolated from the oral cavity of tufted capuchin. | a gram-stain-positive, catalase-negative, coccus-shaped organism was isolated from the oral cavity of tufted capuchin (cebus apella). comparative 16s rrna gene sequence analysis suggested classification of the organism within the genus streptococcus. strain m8t was related most closely to streptococcus oralis atcc 35037t (96.17 % similarity) followed by streptococcus massiliensis ccug 49690t (95.90 %) based on the 16s rrna gene. strain m8t was related most closely to s. massiliensis ccug 49690t ... | 2016 | 26651833 |
| influence of fluoride on the bacterial composition of a dual-species biofilm composed of streptococcus mutans and streptococcus oralis. | despite the widespread use of fluoride for the prevention of dental caries, few studies have demonstrated the effects of fluoride on the bacterial composition of dental biofilms. this study investigated whether fluoride affects the proportion of streptococcus mutans and s. oralis in mono- and dual-species biofilm models, via microbiological, biochemical, and confocal fluorescence microscope studies. fluoride did not affect the bacterial count and bio-volume of s. mutans and s. oralis in mono-spe ... | 2016 | 27643392 |
| streptococcus oralis induces lysosomal impairment of macrophages via bacterial hydrogen peroxide. | streptococcus oralis, an oral commensal, belongs to the mitis group of streptococci and occasionally causes opportunistic infections, such as bacterial endocarditis and bacteremia. recently, we found that the hydrogen peroxide (h2o2) produced by s. oralis is sufficient to kill human monocytes and epithelial cells, implying that streptococcal h2o2 is a cytotoxin. in the present study, we investigated whether streptococcal h2o2 impacts lysosomes, organelles of the intracellular digestive system, i ... | 2016 | 27113357 |
| initial oral biofilm formation on titanium implants with different surface treatments: an in vivo study. | the aim of this study was to examine in vivo the initial bacterial adhesion on titanium implants with different surface treatments. | 2016 | 27232358 |
| corrigendum: lipoteichoic acid of streptococcus oralis uo5: a novel biochemical structure comprising an unusual phosphorylcholine substitution pattern compared to streptococcus pneumoniae. | 2016 | 27103585 | |
| streptococcus oralis cerebral abscess following monkey bite in a 2-month-old infant. | although cerebral abscesses caused by animal bites have been reported, they are extremely rare in infants and have not been described following monkey bite. a 55-day-old male infant presented with a multi-loculated streptococcus oralis cerebral abscess following a monkey bite on the scalp. there was a clinical response to antibiotic therapy and repeated surgical aspiration followed by a ventriculoperitoneal shunt. this is the first report of a patient with a brain abscess following a monkey bite ... | 2016 | 27077615 |
| draft genome sequence of the oral commensal streptococcus oralis 89a with interference activity against respiratory pathogens. | we report the draft genome sequence of the oral commensal streptococcus oralis 89a isolated from the throat of a healthy child during a streptococcal tonsillitis outbreak in umeå, sweden. s. oralis 89a was known to have interference activity against respiratory pathogens in which the colicin v was the potential bacteriocin-encoding gene. | 2016 | 26769934 |
| reduced glutathione mediates resistance to h2s toxicity in oral streptococci. | periodontal disease is associated with changes in the composition of the oral microflora, where health-associated oral streptococci decrease while gram-negative anaerobes predominate in disease. a key feature of periodontal disease-associated anaerobes is their ability to produce hydrogen sulfide (h2s) abundantly as a by-product of anaerobic metabolism. so far, h2s has been reported to be either cytoprotective or cytotoxic by modulating bacterial antioxidant defense systems. although oral anaero ... | 2016 | 26801579 |
| microbial diversity in the early in vivo-formed dental biofilm. | although the mature dental biofilm composition is well studied, there is very little information on the earliest phase of in vivo tooth colonization. progress in dental biofilm collection methodologies and techniques of large-scale microbial identification have made new studies in this field of oral biology feasible. the aim of this study was to characterize the temporal changes and diversity of the cultivable and noncultivable microbes in the early dental biofilm. samples of early dental biofil ... | 2016 | 26746720 |
| differential utilization of basic proline-rich glycoproteins during growth of oral bacteria in saliva. | although saliva is widely recognized as a primary source of carbon and nitrogen for growth of the dental plaque biofilm community, little is known about how different oral bacteria utilize specific salivary components. to address this question, 32 strains representing 16 genera commonly isolated from early plaque biofilms were compared for growth over two transfers in stimulated (by chewing parafilm) whole saliva that was stabilized by heat treatment and dialysis. the cell densities, measured by ... | 2016 | 27316966 |
| in vitro effect of porphyromonas gingivalis methionine gamma lyase on biofilm composition and oral inflammatory response. | methanethiol (methyl mercaptan) is an important contributor to oral malodour and periodontal tissue destruction. porphyromonas gingivalis, prevotella intermedia and fusobacterium nucleatum are key oral microbial species that produce methanethiol via methionine gamma lyase (mgl) activity. the aim of this study was to compare an mgl knockout strain of p. gingivalis with its wild type using a 10-species biofilm co-culture model with oral keratinocytes and its effect on biofilm composition and infla ... | 2016 | 28033374 |
| high-throughput quantitative method for assessing coaggregation among oral bacterial species. | this paper describes a high-throughput method that relies upon a microplate reader to score coaggregation 60 min postmixing, and use of a high-speed real-time imaging technology to describe the rate of coaggregation over time. the results of visual, microplate, and flowcam(™) aggregation scores for oral bacteria streptococcus gordonii, streptococcus oralis, and actinomyces oris, whose ability to coaggregate are well characterized, are compared. following mixing of all possible pairs, the top fra ... | 2016 | 27455031 |
| oral biofilm and caries-infiltrant interactions on enamel. | this study aimed to analyze interactions between oral biofilms and a dental triethylene glycol dimethacrylate (tegdma)-based resin infiltration material on enamel. | 2016 | 26972979 |
| microbial dynamics during conversion from supragingival to subgingival biofilms in an in vitro model. | the development of dental caries and periodontal diseases result from distinct shifts in the microbiota of the tooth-associated biofilm. this in vitro study aimed to investigate changes in biofilm composition and structure, during the shift from a 'supragingival' aerobic profile to a 'subgingival' anaerobic profile. biofilms consisting of actinomyces oris, candida albicans, fusobacterium nucleatum, streptococcus oralis, streptococcus mutans and veillonella dispar were aerobically grown in saliva ... | 2016 | 26033167 |
| interkingdom cooperation between candida albicans, streptococcus oralis and actinomyces oris modulates early biofilm development on denture material. | candida-associated stomatitis affects up to 60% of denture wearers, and candida albicans remains the most commonly isolated fungal species. the oral bacteria actinomyces oris and streptococcus oralis are abundant in early dental plaque. the aims of this study were to determine the effects of s. oralis and a. oris on the development of c. albicans biofilms on denture material. resin discs were coated with saliva and at early (1.5 h) or later (24 h) stages of biofilm development, cell numbers of e ... | 2016 | 26755532 |
| in vitro spectrum of pexiganan activity; bactericidal action and resistance selection tested against pathogens with elevated mic values to topical agents. | pexiganan, in phase 3 clinical development for topical use, exhibited bactericidal activity in vitro against gram-positive and -negative isolates and was also shown to have a low potential for resistance development in broth serial passage experiments. susceptibility studies were performed against bacterial isolates (110 total from 2004 to 2013; primarily from skin and soft tissue infections) selected for elevated mic values (non-wildtype [wt] distributions) to bacitracin, polymyxin b, neomycin, ... | 2016 | 27352729 |
| antimicrobial activity of submicron glass fibres incorporated as a filler to a dental sealer. | two types of antimicrobial glass fibers containing zno and cao, with diameters ranging from tens of nanometers to 1 µm, were successfully fabricated by a laser spinning technique. the antimicrobial performance was corroborated according to iso 20743:2013, by using gram-negative (escherichia coli) and gram-positive (streptococcus oralis, streptococcus mutans and staphylococcus aureus) bacteria, and yeast (candida krusei) (more than 3 logs of reduction). the metabolic activity and endosomal system ... | 2016 | 27509353 |
| impact of implant-abutment connection on osteoimmunological and microbiological parameters in short implants: a randomized controlled clinical trial. | the study aimed to determine the levels of soluble receptor activator of nuclear factor-кb ligand (srankl) and osteoprotegerin (opg) as well as their relative calculated ratio in peri-implant crevicular fluid (picf) obtained around two different types of implant-abutment connection on short implants following a 12-month monitoring period. moreover, the levels of a number of oral bacterial species were investigated in the corresponding submucosal biofilm samples. | 2016 | 27469171 |
| 5-fluorouracil sensitivity varies among oral micro-organisms. | 5-fluorouracil (5-fu), a commonly used chemotherapeutic agent, often causes oral mucositis, an inflammation and ulceration of the oral mucosa. micro-organisms in the oral cavity are thought to play an important role in the aggravation and severity of mucositis, but the mechanisms behind this remain unclear. although 5-fu has been shown to elicit antibacterial effects at high concentrations (>100 µm), its antibacterial effect at physiologically relevant concentrations in the oral cavity is unknow ... | 2016 | 27296270 |
| a dual-species microbial model for studying the dynamics between oral streptococci and periodontal pathogens during biofilm development on titanium surfaces by flow cytometry. | the association of the pioneer organisms streptococcus mutans atcc 25175 or streptococcus oralis atcc 9811 with secondary colonizers fusobacterium nucleatum atcc 25586 or porphyromonas gingivalis atcc 33277 during biofilm development on titanium surfaces was evaluated by flow cytometry (fcm) using specific polyclonal antibodies. elisa and fcm were employed, revealing high antibody sensitivity and specificity. biofilm formation of four dual-species combinations was analyzed by crystal violet stai ... | 2016 | 27032997 |
| characterization of biosurfactants produced by lactobacillus spp. and their activity against oral streptococci biofilm. | lactic acid bacteria (lab) can interfere with pathogens through different mechanisms; one is the production of biosurfactants, a group of surface-active molecules, which inhibit the growth of potential pathogens. in the present study, biosurfactants produced by lactobacillus reuteri dsm 17938, lactobacillus acidophilus dds-1, lactobacillus rhamnosus atcc 53103, and lactobacillus paracasei b21060 were dialyzed (1 and 6 kda) and characterized in term of reduction of surface tension and emulsifying ... | 2016 | 27102127 |
| development of long-term antimicrobial poly(methyl methacrylate) by incorporating mesoporous silica nanocarriers. | poly(methyl methacrylate) (pmma) used as removable denture bases or orthodontic appliances has relatively poor antimicrobial properties, which accelerate oral infection and induce unfavorable odors. mesoporous silica nanoparticles (msns) have been highlighted as a potential additive to overcome this issue because of their drug-loading capacity. here, we present the long-term antimicrobial effect of msn-incorporated pmma with drug-loading capacity. | 2016 | 27671462 |
| investigation of acrylic resin disinfection using chemicals and ultrasound. | dental prosthetic and orthodontic appliances are transported from the clinic to the laboratory for additions and repairs. these appliances, containing microbes from the oral flora, are a high risk for cross-contamination. the aim of this study was to evaluate the effect of chemical and ultrasound disinfection against two in vitro biofilms and an in vivo formed biofilm grown on unprepared and polished polymethyl methacrylate (pmma) surfaces. | 2016 | 27244668 |
| streptococcus oralis and candida albicans synergistically activate μ-calpain to degrade e-cadherin from oral epithelial junctions. | streptococcus oralis forms robust mucosal biofilms with candida albicans that have increased pathogenic potential. in this study, using oral epithelial cultures, organotypic oral mucosal constructs, and a mouse model of oral infection, we demonstrated that s. oralis augmented c. albicans invasion through epithelial junctions. c. albicans and s. oralis decreased epithelial e-cadherin levels by synergistically increasing µ-calpain, a proteolytic enzyme that targets e-cadherin. in the mouse coinfec ... | 2016 | 27190184 |
| effects of surface reaction-type pre-reacted glass ionomer on oral biofilm formation of streptococcus gordonii. | streptococcus gordonii, a bacterium involved in the initial colonization of tooth surfaces, contributes to dental biofilm formation and is an important cause of infective endocarditis. this study aimed to investigate the influence of surface reaction-type pre-reacted glass ionomer (s-prg) filler on oral bacterial growth and aggregation of s. gordonii. the effect of various concentrations of s-prg eluate on the growth and the biofilm formation of s. gordonii and other oral microorganisms (strepto ... | 2016 | 26319990 |
| arthritis-induced alveolar bone loss is associated with changes in the composition of oral microbiota. | rheumatoid arthritis (ra) and periodontitis (pd) are chronic inflammatory disorders that cause bone loss. pd tends to be more prevalent and severe in ra patients. previous experimental studies demonstrated that ra triggers alveolar bone loss similarly to pd. the aim of this study was to investigate if arthritis-induced alveolar bone loss is associated with modification in the oral microbiota. checkerboard dna-dna hybridization was employed to analyze forty oral bacterial species in 3 groups of c ... | 2016 | 26996070 |
| antimicrobial effects of commensal oral species are regulated by environmental factors. | the objectives of this study are to identify oral commensal species which can inhibit the growth of the main periodontopathogens, to determine the antimicrobial substances involved in these inhibitory activities and to evaluate the influence of environmental factors on the magnitude of these inhibitions. | 2016 | 26875613 |
| chemical characterization and antioxidant, antimicrobial, and anti-inflammatory activities of south brazilian organic propolis. | south brazilian organic propolis (op), which has never been studied before, was assessed and its chemical composition, scavenging potential of reactive oxygen species, antimicrobial and anti-inflammatory activities are herein presented. based on the chemical profile obtained using hplc, op was grouped into seven variants (op1-op7) and all of them exhibited high scavenging activity, mainly against superoxide and hypochlorous acid species. op1, op2, and op3 had the smallest minimal inhibitory conc ... | 2016 | 27802316 |
| dentifrice containing extract of rosmarinus officinalis linn.: an antimicrobial evaluation. | this study aimed to evaluate the antimicrobial activity of a dentifrice containing an alcoholic extract of rosemary on oral bacteria, compared to a commercially available herbal dentifrice. standard strains of streptococcus mutans (atcc 25175), streptococcus oralis (atcc 9811) and lactobacillus rhamnosus (atcc 7469) were used, as well as different toothpastes based on rosemary (tr), on propolis (th), triclosan (positive control) (tpc) and non-fluoridated dentifrice (negative control) (tnc). bact ... | 2016 | 27982224 |
| flavanone and isoflavone glucosylation by non-leloir glycosyltransferases. | flavonoids possess a wide range of biological activities. their glycosylation is of considerable interest, as it often positively influences their pharmacokinetic and other molecular properties. we recently showed that two non-leloir glycosyltransferases that use sucrose as carbohydrate donor, an amylosucrase from neisseria polysaccharea (ams-np) and a glucansucrase from streptococcus oralis (gtfr-so), were hardly able to glucosylate flavones, but accepted flavanes as substrates. we now examined ... | 2016 | 27374405 |
| re-evaluation of the taxonomy of the mitis group of the genus streptococcus based on whole genome phylogenetic analyses, and proposed reclassification of streptococcus dentisani as streptococcus oralis subsp. dentisani comb. nov., streptococcus tigurinus as streptococcus oralis subsp. tigurinus comb. nov., and streptococcus oligofermentans as a later synonym of streptococcus cristatus. | the mitis group of the genus streptococcus currently comprises 20 species with validly published names, including the pathogen s. pneumoniae. they have been the subject of much taxonomic confusion, due to phenotypic overlap and genetic heterogeneity, which has hampered a full appreciation of their clinical significance. the purpose of this study was to critically re-examine the taxonomy of the mitis group using 195 publicly available genomes, including designated type strains for phylogenetic an ... | 2016 | 27534397 |
| acidogenicity and acid tolerance of streptococcus oralis and streptococcus mitis isolated from plaque of healthy and incipient caries teeth. | non-mutans low ph oral streptococci are postulated to contribute to caries etiology. | 2016 | 27790973 |
| pl3 amidase, a tailor-made lysin constructed by domain shuffling with potent killing activity against pneumococci and related species. | the emergence and spread of antibiotic-resistant bacteria is pushing the need of alternative treatments. in this context, phage therapy is already a reality to successfully fight certain multiresistant bacteria. among different phage gene products, murein hydrolases responsible of phage progeny liberation (also called lysins or endolysins) are weapons that target specific peptidoglycan bonds, leading to lysis and death of susceptible bacteria when added from the outside. in the pneumococcal syst ... | 2016 | 27516758 |
| autoinducer-2 detection among commensal oral streptococci is dependent on ph and boric acid. | autoinducer-2, considered a universal signaling molecule, is produced by many species of bacteria; including oral strains. structurally, autoinducer-2 can exist bound to boron (borated autoinducer-2). functionally, autoinducer-2 has been linked to important bacterial processes such as virulence and biofilm formation. in order to test production of autoinducer-2 by a given bacterial strain, a bioassay using marine bioluminescent bacteria vibrio harveyi as a reporter for autoinducer-2 has been des ... | 2016 | 27350615 |
| highly variable streptococcus oralis strains are common among viridans streptococci isolated from primates. | viridans streptococci were obtained from primates (great apes, rhesus monkeys, and ring-tailed lemurs) held in captivity, as well as from free-living animals (chimpanzees and lemurs) for whom contact with humans is highly restricted. isolates represented a variety of viridans streptococci, including unknown species. streptococcus oralis was frequently isolated from samples from great apes. genotypic methods revealed that most of the strains clustered on separate lineages outside the main cluster ... | 2017 | 27303717 |
| streptococcus oralis neuraminidase modulates adherence to multiple carbohydrates on platelets. | adherence to host surfaces is often mediated by bacterial binding to surface carbohydrates. although it is widely appreciated that some bacterial species express glycosidases, previous studies have not considered whether bacteria bind to multiple carbohydrates within host glycans as they are modified by bacterial glycosidases. streptococcus oralis is a leading cause of subacute infective endocarditis. binding to platelets is a critical step in disease; however, the mechanisms utilized by s. oral ... | 2017 | 27993975 |
| development of a flow chamber system for the reproducible in vitro analysis of biofilm formation on implant materials. | since the introduction of modern dental implants in the 1980s, the number of inserted implants has steadily increased. implant systems have become more sophisticated and have enormously enhanced patients' quality of life. although there has been tremendous development in implant materials and clinical methods, bacterial infections are still one of the major causes of implant failure. these infections involve the formation of sessile microbial communities, called biofilms. biofilms possess unique ... | 2017 | 28187188 |
| detection and quantification of aggregatibacter actinomycetemcomitans, porphyromonas gingivalis and streptococcus oralis in blood samples with different microbiological identification methods: an in vitro study. | culture-based methods (culture broth bottles or lysis methods) have been the standard for detecting bacteremia. more recently, quantitative polymerase chain reaction (qpcr) was proposed as a more sensitive and specific test although none of them has been validated for the identification of periodontal pathogens (fastidious growing bacteria) in blood samples. | 2017 | 27875793 |
| antimicrobial effects of garcinia mangostana on cariogenic microorganisms. | garcinia mangostana commonly called as mangosteen fruit has been used as an antibacterial agent since age old times. the mangosteen pericarp has proven to have antibacterial effect, but the effect of the same on cariogenic organisms has not been explored. the present study was an attempt to gain a better understanding of the antibacterial effect of mangosteen pericarp on the cariogenic bacteria, to unravel the therapeutic potential for the same. | 2017 | 28274037 |
| eugenia uniflora dentifrice for treating gingivitis in children: antibacterial assay and randomized clinical trial. | school-age children are frequently at high risk for the onset of biofilm-dependent conditions, including dental caries and periodontal diseases. the objective of this study was to evaluate the clinical efficacy of a dentifrice containing eugenia uniflora linn. (surinam cherry) extract versus a triclosan-based comparator in treating gingivitis in children aged 10-12 years. the in vitro antibacterial potential of the dentifrice was tested against oral pathogens (streptococcus mutans, streptococcus ... | 2017 | 27652698 |
| live and heat-killed lactobacillus spp. interfere with streptococcus mutans and streptococcus oralis during biofilm development on titanium surface. | this research investigates the ability of live and heat-killed (hk) lactic acid bacteria (lab) to interfere with streptococcus mutans atcc 25175 and streptococcus oralis atcc 9811 during biofilm formation. | 2017 | 28193570 |
| effect of photodynamic therapy in combination with various irrigation protocols on an endodontic multispecies biofilm ex vivo. | to analyse the antibacterial effect of photodynamic therapy (pdt) in combination with various irrigation protocols on a multispecies biofilm in root canals ex vivo. | 2017 | 28276583 |
| effect of endodontic irrigants on biofilm matrix polysaccharides. | to specifically investigate the effect of endodontic irrigants at their clinical concentration on matrix polysaccharides of cultured biofilms. | 2017 | 26705856 |
| interactions between streptococcus oralis, actinomyces oris, and candida albicans in the development of multispecies oral microbial biofilms on salivary pellicle. | the fungus candida albicans is carried orally and causes a range of superficial infections that may become systemic. oral bacteria actinomyces oris and streptococcus oralis are abundant in early dental plaque and on oral mucosa. the aims of this study were to determine the mechanisms by which s. oralis and a. oris interact with each other and with c. albicans in biofilm development. spatial distribution of microorganisms was visualized by confocal laser scanning microscopy of biofilms labeled by ... | 2017 | 26834007 |
| an oral multispecies biofilm model for high content screening applications. | peri-implantitis caused by multispecies biofilms is a major complication in dental implant treatment. the bacterial infection surrounding dental implants can lead to bone loss and, in turn, to implant failure. a promising strategy to prevent these common complications is the development of implant surfaces that inhibit biofilm development. a reproducible and easy-to-use biofilm model as a test system for large scale screening of new implant surfaces with putative antibacterial potency is therefo ... | 2017 | 28296966 |
| rhamnolipids from non-pathogenic burkholderia thailandensis e264: physicochemical characterization, antimicrobial and antibiofilm efficacy against oral hygiene related pathogens. | biosurfactants are naturally occurring surface active compounds that have mainly been exploited for environmental applications and consumer products, with their biomedical efficacy an emerging area of research. rhamnolipids area major group of biosurfactants that have been reported for their antimicrobial and antibiofilm efficacy. one of the main limiting factors for scaled up production and downstream applications of rhamnolipids is the fact that they are predominantly produced from the opportu ... | 2017 | 28065676 |
| bactericidal effect of photocatalytically-active nanostructured tio2 surfaces on biofilms of the early oral colonizer, streptococcus oralis. | this study evaluated the photocatalytic bactericidal effect of nanostructured anatase-rich titanium dioxide (tio2 ) on biofilms. commercially pure titanium discs were spin-coated with photocatalytic tio2 nanoparticles (p25). uncoated discs were used as control (ctrl). half of the ctrl and half of the p25-coated surfaces were coated with purified saliva (sal) to give four different groups (ctrl, ctrl+sal, p25 and p25+sal). streptococcus oralis were allowed to form biofilms on the discs for 18 hou ... | 2017 | 28380676 |
| development of laser-structured liquid-infused titanium with strong biofilm-repellent properties. | medical implants are commonly used in modern medicine but still harbor the risk of microbial infections caused by bacterial biofilms. as their retrospective treatment is difficult, there is a need for biomedical materials that inhibit bacterial colonization from the start without using antibacterial agents, as these can promote resistance development. the promising concept of slippery liquid-infused porous surfaces (slips) possesses enormous potential for this purpose. in the present study, this ... | 2017 | 28229593 |
| microarray analysis of macrophage response to infection with streptococcus oralis reveals the immunosuppressive effect of hydrogen peroxide. | oral streptococci including mitis group streptococci are commensal residents and are also the first to colonize the oral cavity. however, various species of these oral streptococci have the potential to invade the host and occasionally lead to severe infectious disease such as cardiovascular diseases. oral streptococci have close interactions with the host immune system including macrophages at the oral mucosal surface. one notable common trait of oral streptococcus including streptococcus orali ... | 2017 | 28202416 |
| antimicrobial effects of mastic extract against oral and periodontal pathogens. | various antimicrobial agents are widely used in the therapy of oral inflammatory diseases. however, their side effects and the appearance of drug resistance justify research on natural antimicrobial agents to target oral pathogens that are safe for the host. in the present study, antimicrobial properties of mastic extract on commensal and pathogenic oral bacteria, as well as its possible cytotoxic effect toward cells of epithelial and mesenchymal origin, were evaluated and compared with the comm ... | 2017 | 28067105 |
| electron transport chain is biochemically linked to pilus assembly required for polymicrobial interactions and biofilm formation in the gram-positive actinobacterium actinomyces oris. | the gram-positive actinobacteria actinomyces spp. are key colonizers in the development of oral biofilms due to the inherent ability of actinomyces to adhere to receptor polysaccharides on the surface of oral streptococci and host cells. this receptor-dependent bacterial interaction, or coaggregation, requires a unique sortase-catalyzed pilus consisting of the pilus shaft fima and the coaggregation factor cafa forming the pilus tip. while the essential role of the sortase machine srtc2 in pilus ... | 2017 | 28634238 |
| rechargeable microbial anti-adhesive polymethyl methacrylate incorporating silver sulfadiazine-loaded mesoporous silica nanocarriers. | even though polymethyl methacrylate (pmma) resin is widely used as a dental material, it has poor microbial anti-adhesive properties, which accelerates oral infections. in this investigation, silver-sulfadiazine (agsd)-loaded mesoporous silica nanoparticles (ag-msns) were incorporated into pmma to introduce long-term microbial anti-adhesive effects and to make pmma a rechargeable resin. | 2017 | 28778494 |
| the sixth sensor: a candida albicans biofilm master regulator that responds to inter-kingdom interactions. | 2017 | 28700262 | |
| antimicrobial potential of plant extracts and chemical fractions of sideroxylon obtusifolium (roem. & schult.) t.d. penn on oral microorganisms. | the aim of this study is to evaluate the in vitro antimicrobial activity of plant extracts and chemical fractions of sideroxylon obtusifolium t.d. penn on streptococcus mutans, streptococcus oralis, streptococcus salivarius, streptococcus parasanguinis, and candida albicans as well as to identify the chemical classes found in the bioactive extracts possessing better activity. | 2017 | 28512279 |
| s. oralis activates the efg1 filamentation pathway in c. albicans to promote cross-kingdom interactions and mucosal biofilms. | candida albicans and streptococcus oralis are ubiquitous oral commensal organisms. under host-permissive conditions these organisms can form hypervirulent mucosal biofilms. c. albicans biofilm formation is controlled by 6 master transcriptional regulators: bcr1, brg1, efg1, tec1, ndt80, and rob1. the objective of this work was to test whether any of these regulators play a role in cross-kingdom interactions between c. albicans and s. oralis in oral mucosal biofilms, and identify downstream targe ... | 2017 | 28481721 |
| top-down lesa mass spectrometry protein analysis of gram-positive and gram-negative bacteria. | we have previously shown that liquid extraction surface analysis (lesa) mass spectrometry (ms) is a technique suitable for the top-down analysis of proteins directly from intact colonies of the gram-negative bacterium escherichia coli k-12. here we extend the application of lesa ms to gram-negative pseudomonas aeruginosa ps1054 and gram-positive staphylococcus aureus mssa476, as well as two strains of e. coli (k-12 and bl21 mcherry) and an unknown species of staphylococcus. moreover, we demonstr ... | 2017 | 28681361 |
| in vitro studies of nanosilver-doped titanium implants for oral and maxillofacial surgery. | the addition of an antibacterial agent to dental implants may provide the opportunity to decrease the percentage of implant failures due to peri-implantitis. for this purpose, in this study, the potential efficacy of nanosilver-doped titanium biomaterials was determined. titanium disks were incorporated with silver nanoparticles over different time periods by tollens reaction, which is considered to be an eco-friendly, cheap, and easy-to-perform method. the surface roughness, wettability, and si ... | 2017 | 28652733 |
| liver abscess caused by pannonibacter phragmitetus: case report and literature review. | bacterial hepatic abscess is a common occurrence in developing countries, which is mostly caused by klebsiella pneumoniae and escherichia coli. pannonibacter phragmitetus is a gram-negative alkali-tolerant bacillus that exists in the natural environment. human infection by this bacterium is rare, with only four cases reported. | 2017 | 28487855 |
| biomarkers and bacteria around implants and natural teeth in the same individuals. | this cross-sectional study assesses cytokine levels in peri-implant crevicular fluid (picf)/gingival crevicular fluid (gcf) and a selection of subgingival/submucosal plaque bacteria from clinically healthy or diseased sites in the same individuals. | 2017 | 28440740 |
| antimicrobial effect of leaves of phyllanthus niruri and solanum nigrum on caries causing bacteria: an in vitro study. | solanum nigrum and phyllanthus niruri are common herbs which are indigeneous to india. solanum nigrum commonly called 'manathakkali keerai' in tamil, forms an indispensable part of south indian diet. phyllanthus niruri (keezhanelli in tamil) is a widely used medicinal plant, the leaves of which have been used extensively in ayurveda and native medicine to cure various liver ailments. the herbs solanumnigrum and phyllanthus niruri have been found to be effective against numerous enteropathogens i ... | 2017 | 28764202 |
| extending the scope of gtfr glucosylation reactions with tosylated substrates for rare sugars synthesis. | functionalized rare sugars were synthesized with 2-, 3- and 6-tosylated glucose derivatives as acceptor substrates by transglucosylation with sucrose and the glucansucrase gtfr from streptococcus oralis. the 2- and 3-tosylated glucose derivatives yielded the corresponding 1,6-linked disaccharides (isomaltose analogues) whereas the 6-tosylated glucose derivatives resulted in 1,3-linked disaccharides (nigerose analogue) with high regioselectivity in up to 95% yield. docking studies provide insight ... | 2017 | 28796424 |
| bacteriotherapy with streptococcus salivarius 24smb and streptococcus oralis 89a nasal spray for preventing recurrent acute otitis media in children: a real-life clinical experience. | recurrence of acute otitis media (raom) is a relevant issue in the clinical practice. "bacteriotherapy" has been proposed as an option in children with raom. streptococcus salivarius 24smb nasal spray has been previously demonstrated to reduce the risk of acute otitis media (aom) in otitis-prone children. the current retrospective and observational study aimed to confirm this outcome in a real-life setting, such as the common pediatric practice. group a (108 children) served as control; group b ... | 2017 | 28684920 |
| streptococcus tigurinus is frequent among gtfr-negative streptococcus oralis isolates and in the human oral cavity, but highly virulent strains are uncommon. | streptococcus tigurinus is a new member of the mitis group and is associated with infective endocarditis. low and high virulent variants have been described. a search was made in the national reference collection of endocarditis isolates for s. tigurinus-like strains by sequencing housekeeping genes (16s rrna-gene, gdh, groel, soda). the strains were further profiled by polymerase chain reaction (pcr) targeting a choice of virulence genes (rib-like, csha-like, gtfr, int, pita, hyla). to study th ... | 2017 | 28473881 |