Publications

analysis of edta-soluble cell surface components of gram-positive anaerobic cocci.	the protein content of edta extracts from 76 strains of gram-positive anaerobic cocci was examined using sds-page. strains of peptostreptococcus anaerobius produced almost identical profiles; greater heterogeneity was observed within the species peptococcus magnus, peptococcus prevotii and peptococcus asaccharolyticus, but several strains within each biotype produced similar patterns. serological investigation of these extracts by elisa revealed numerous cross-reactions among the different bioty ...	1986	3806050
increased resistance towards two systemic experimental infections by tetrachlorodecaoxygen anion complex. possible implications of cellular and humoral immunity.	the tetrachlorodecaoxygen anion complex (tcdo, oxoferin) given intravenously 1 h after intravenous infection of mice with candida albicans increased the host's resistance gradually in doses up to 3.1 mumol/kg body weight (b.w.). above this level, the stimulatory effect decreased, turning to an inhibition at a dose of 18.6 mumol/kg b.w. tcdo. repeated applications of the optimum single dose had no cumulative effect. in experimental infections with the strictly anaerobic peptostreptococcus interme ...	1986	3566839
[anaerobic bacteria in various infections and their antibiotic susceptibility patterns].	in this study we have examined antibiotic susceptibility of anaerobic bacteria isolated from various samples of 75 patients. anaerobic bacteria from 22 (29.3%) patients, and only aerobic bacteria from 29 (38.7%) patients have been isolated; there has been no growth in cultures from 24 (32%) patients. of 75 isolates from 51 patients 38 (50.6%) are anaerobic and 37 (49.4%) are aerobic bacteria. following anaerobic bacteria have been isolated: peptostreptococcus (13), peptococcus (5), microaerophil ...	1986	3657617
sulbactam/ampicillin versus metronidazole/gentamicin in the treatment of severe pelvic infections.	the clinical efficacy and safety of sulbactam/ampicillin versus metronidazole/gentamicin were compared in 39 patients with severe pelvic infections. 30 patients had severe acute pelvic inflammatory disease with peritonitis, 3 tubo-ovarian abscesses, 4 endomyometritis, and 2 posthysterectomy pelvic cellulitis. aerobic and anaerobic cultures from the sites of infection yielded 259 micro-organisms from 38 patients; an average of 6.8 bacteria per infection (3.9 anaerobes and 2.9 aerobes). the most f ...	1986	3013566
efficacy of sulbactam plus ampicillin in gynecologic infections.	the efficacy of sulbactam plus ampicillin in the treatment of various gynecologic infections was evaluated in 24 women (median age, 35 years). ten women had pelvic cellulitis plus vaginal cuff abscess; six, pyeloperitonitis; three, vaginal cuff abscess; three, surgical wound sepsis; one, tubo-ovarian abscess; and one, endometritis. surgical procedures preceding infection included abdominal hysterectomy, ovarian cyst removal, ectopic pregnancy, correction of cystocele, and uterine dilatation and ...	1986	3026007
the significance of clostridial isolates in intra-abdominal sepsis.	in order to evaluate the significance of clostridial species in intra-abdominal infections, the bacteriology records of three hospitals were reviewed during a period of five years. included in this report were 41 patients from whom clostridial species were recovered from specimens of free peritoneal fluid, abscess cavities or bile. seven patients died for a mortality rate of 17.1 per cent. most patients had polymicrobial infections of which clostridial organisms were one of the several anaerobes ...	1986	2876527
pathogenicity of clostridium species with other bacteria in mixed infections.	the relationship of clostridial isolates with other bacteria in mixed infections was studied by means of a subcutaneous abscess model in mice. we used 26 isolates of seven clostridial species, two bacteroides spp., eight gram-positive facultative or anaerobic cocci and three enteric gram-negative aerobic rods. abscesses were induced by all seven clostridium perfringens and three c. butyricum isolates and by some of the others. selective antimicrobial therapy experiments showed that enteric gram- ...	1986	2878957
ampicillin/sulbactam versus metronidazole-gentamicin in the treatment of soft tissue pelvic infections.	the clinical efficacy and safety of ampicillin/sulbactam versus metronidazole-gentamicin were evaluated in a comparative, randomized, prospective study. forty-four patients were enrolled: 22 received the ampicillin/sulbactam regimen, and 22 received the metronidazole-gentamicin combination. there were 33 cases of severe acute pelvic inflammatory disease, two tuboovarian abscesses, five cases of endomyometritis, and two cases of posthysterectomy pelvic cellulitis. aerobic and anaerobic cultures f ...	1987	3030109
effect of high-fiber and high-oil diets on the fecal flora of swine.	six pairs of pigs were fed a basal diet, a high-fiber diet, and a diet high in corn oil in different sequences to minimize the carry-over effect of diet. after 2 months on each diet, a fecal specimen from each pig was cultured on nonselective medium in roll tubes. fifty colonies were randomly selected from each fecal sample, and isolates were characterized to identify a representative cross section of the fecal flora. the bacterial composition of the fecal flora differed between basal and high-f ...	1987	2821900
controlled evaluation of trypticase soy broth with and without gelatin and yeast extract in the detection of bacteremia and fungemia.	the addition of gelatin to blood culture media has been suggested to prevent the inhibition of neisseria meningitidis, neisseria gonorrhoeae, gardnerella vaginalis, and peptostreptococcus anaerobius that is caused by sodium polyanetholsulfonate. to determine the effect of such supplementation on the overall yield of microorganisms, we compared the yield and speed of detection of clinically important microorganisms from 5422 paired 10-ml samples of blood cultured in trypticase soy broth (tsb) con ...	1987	2831009
comparative activity of ly146032 against anaerobic cocci.	fifty strains of anaerobic gram-positive cocci were tested in vitro against benzylpenicillin, teicoplanin, vancomycin and ly146032. the organisms displayed a wide range of susceptibility to penicillin and the minimum inhibitory concentration for 11 of the strains was greater than or equal to 1 mg penicillin/l. the activity of teicoplanin exceeded that of vancomycin by a factor of two. the activity of ly 146032 varied in different culture media and was dramatically potentiated by the addition of ...	1987	2831046
[clinical studies on cefuzonam in obstetrics and gynecological infections].	to evaluate the usefulness and the safety of cefuzonam (czon, l-105) against obstetric and gynecological infections, we conducted clinical trials and obtained the results summarized as follows. 1. thirteen patients with obstetric and gynecological infections (intrauterine infection 2 cases, adnexitis 7, 1 case each of abscess of adnexa uteri, abscess of the vaginal wall, pyelonephritis, and mammitis) were treated with czon. the czon was administered by intravenous injection or intravenous drip i ...	1987	3669283
identification of anaerobic bacteria using high-performance liquid chromatography.	20 species of the genera clostridium, bacteroides, fusobacterium and the type strains of peptostreptococcus anaerobius, peptococcus asaccharolyticus, veillonella parvula and propionibacterium acnes were examined for the production of volatile (vfa) and nonvolatile (nvfa) short-chain fatty acids using high-performance liquid chromatography (hplc) with a column for organic acids (aminex hpx-87h). 10 min are needed for sample preparation and the vfa and nvfa were detected simultaneously in a single ...	1987	3673341
interactions of ciprofloxacin with clindamycin, metronidazole, cefoxitin, cefotaxime, and mezlocillin against gram-positive and gram-negative anaerobic bacteria.	a total of 598 clinical isolates of anaerobic bacteria were tested against ciprofloxacin by the agar dilution technique with 10(5) cfu on wilkins-chalgren medium. selected strains representative of the six major genera of anaerobes relatively resistant to the quinolones were tested for interactions with ciprofloxacin in combination with clindamycin, metronidazole, cefoxitin, cefotaxime, or mezlocillin by using a checkerboard agar dilution technique. cefotaxime-ciprofloxacin and clindamycin-cipro ...	1987	3674848
enrichment of subgingival microflora on human serum leading to accumulation of bacteroides species, peptostreptococci and fusobacteria.	this study was undertaken to identify ecological factors that favour opportunistic pathogenic species in the subgingival microflora. in a first approach, human serum as a substitute for gingival exudate, was used for batch-wise enrichment of subgingival plaque. the microflora resulting after 5-6 enrichment steps consisted of black-pigmented and non-black-pigmented bacteroides species, peptostreptococcus micros and fusobacterium nucleatum as the main organisms. it is noted that the same group of ...	1987	3674857
the in-vitro activity of doxycycline and minocycline against anaerobic bacteria.	the likelihood of bacterial resistance now prevents the use of oxytetracycline in the empirical therapy of anaerobic infections. this study investigates the in-vitro activity of two semi-synthetic derivatives, doxycycline and minocycline, against a range of anaerobic bacteria. mics for each antibiotic were determined by an agar incorporation technique. doxycycline and minocycline were four to eight times more active against the majority of strains than oxytetracycline. with the exception of bact ...	1987	3680075
in vitro activity of ampicillin plus sulbactam against anaerobes compared to ampicillin and cefoxitin.	the antimicrobial susceptibility of 195 recent clinical isolates of anaerobic bacteria was studied to ampicillin alone, ampicillin + 1 mg/l sulbactam, ampicillin + 5 mg/l sulbactam, and cefoxitin by means of agar dilution tests. the ampicillin-sulbactam combinations were the most effective drugs against species of the bacteroides fragilis group, the mic90 of ampicillin + 5 mg/l sulbactam for b. fragilis being less than 1 mg/l, compared to 256 mg/l of ampicillin, 4 mg/l of ampicillin + 1 mg/l sul ...	1987	3692611
viability of lyophilized anaerobes in two media.	viability of 15 species of anaerobes was followed after freeze-drying and storage for 1 year. organisms maintained in a 12% sucrose concentration in chopped meat carbohydrate broth survived longer and maintained higher viability counts than those organisms in double-strength skim milk.	1987	3568744
heparinase production by anaerobic bacteria.	the production of heparinase by a wide range of anaerobic bacteria isolated from clinical specimens was investigated. none of the 29 strains of bacteroides fragilis produced heparinase. of 62 other bacteroides tested, only two of four strains of b ovatus, two of three strains of b thetaiotaomicron, and two of four strains of b uniformis were heparinase producers. none of the 48 strains of fusobacteria or seven strains of veillonella produced heparinase. the anaerobic cocci (19 peptococci and sev ...	1987	3584480
rapid identification of obligately anaerobic gram-positive cocci using high-performance liquid chromatography.	high-performance liquid chromatography was evaluated as a rapid means of identifying obligately anaerobic gram-positive cocci of medical interest. isolates were inoculated into a defined chemical medium consisting primarily of amino acids and were incubated aerobically for 1 h at 35 degrees c. after removal of organisms, the supernatant fluids were derivatized for 1 min at room temperature by the addition of o-phthalaldehyde. the total time required to run a chromatogram was approximately 50 min ...	1987	3597760
microbiology of retropharyngeal abscesses in children.	aspiration of retropharyngeal abscesses was performed in 14 children. cultures were taken from aspirates for aerobic and anaerobic bacteria, and all yielded bacterial growth. anaerobes were isolated in all patients; they were the only organisms isolated in two patients (14%) and were mixed with aerobes in 12 patients (86%). there were 78 anaerobic isolates (5.6 per specimen). the predominant anaerobes were bacteroides species, peptostreptococcus species, and fusobacterium species. there were 26 ...	1987	3812388
bacteraemia and seeding of capsulate bacteroides spp. and anaerobic cocci.	the effect of capsulation on the ability of bacteroides fragilis, b. asaccharolyticus and anaerobic gram-positive cocci to induce bacteraemia and seeding to various organs was investigated. the test species were injected into mice subcutaneously alone, or mixed with other aerobic or facultative organisms. capsulate anaerobes were isolated more frequently from the blood, spleen, liver, and kidneys of infected animals than were non-capsulate organisms. after injection of single anaerobic strains, ...	1987	3820272
increased faecal glycosidases in patients with crohn's disease.	the strictly anaerobic faecal flora of patients with crohn's disease (cd) contains higher numbers of coccoid rods (eubacterium, peptostreptococcus and coprococcus species) and gram-negative rods (bacteroides vulgatus) than the flora of healthy subjects. the abnormal flora of patients with cd might affect levels of glycolytic enzymes and metabolic products and thus play some role in the pathogenesis of cd. therefore, the activity of 23 glycosidases that can degrade mucus glycoproteins or plant po ...	1987	3609504
controlled evaluation of modified radiometric blood culture medium supplemented with gelatin for detection of bacteremia and fungemia.	although the addition of 1.2% gelatin to broth blood culture media containing sodium polyanetholesulfonate has been shown to enhance detection of certain bacteria, including neisseria meningitidis, n. gonorrhoeae, peptostreptococcus anaerobius, and gardnerella vaginalis, the effect of such supplementation on the detection of other microorganisms causing bacteremia and fungemia is not known. therefore, we studied bactec 6b medium with and without gelatin in 6,833 paired comparisons to examine the ...	1987	3624437
myonecrosis of the abdominal wall, complicating radical hysterectomy.		1987	2978389
pathogenicity and therapy of anaerobic bacteria in upper respiratory tract infections.		1987	3547288
use of intravenous ciprofloxacin in difficult-to-treat infections.	intravenous ciprofloxacin was administered to 54 patients who were either critically ill or in whom oral administration was not possible. the 31 males and 23 females ranged in age from 20 to 89 years (mean, 53.2 +/- 17.8 years). patients had "difficult-to-treat" infections, i.e., respiratory infections (15), abscesses (four intraabdominal, three lung, two soft tissue, and one intrahepatic), deep soft tissue infections (10), chronic post-traumatic osteomyelitis in exacerbation (nine), upper urina ...	1987	3555059
comparative activity of metronidazole and tinidazole against clostridium difficile and peptostreptococcus anaerobius.	broth dilution mics of metronidazole against 38 strains of clostridium difficile (0.13 microgram/ml) and 11 strains of peptostreptococcus anaerobius (0.11 microgram/ml) were lower than those of tinidazole (0.16 and 0.17 microgram/ml, respectively). as molar concentrations the nitroimidazoles were equally active. the mics of the two drugs correlated positively, and the relative activity of tinidazole increased with decreasing susceptibility of both species.	1987	3566248
the intraocular environment and experimental anaerobic bacterial endophthalmitis.	anaerobic bacteria are prevalent in conjunctival flora but have not been adequately investigated as possible causes of endophthalmitis. the mean oxidation-reduction potential (eh) of the rabbit vitreous was found to be +25.1 mv, well within the limiting eh value of many anaerobes. there was an oxygen pressure gradient in the vitreous ranging from 2.1 mm hg immediately posterior to the lens to approximately 20 mm hg adjacent to the medullary ray. endophthalmitis was produced with pure cultures of ...	1987	3499883
microbiological studies of tracheostomy site wounds.	specimens were obtained from 25 patients who developed tracheostomy site wound infections. aerobic bacteria only were isolated in 4 (16%) instances, anaerobic bacteria only in 2 (8%), and mixed aerobic and anaerobic isolates were recovered in 19 (76%). a total of 145 isolates (72 aerobes and 73 anaerobes) were recovered, an average of 5.8 isolates per specimen (2.9 aerobes and 2.9 anaerobes). the most frequently recovered isolates were peptostreptococcus sp., bacteroides sp., alpha-hemolytic str ...	1987	3502089
intraventricular haemorrhage complicating a brain abscess.	intraventricular haemorrhage occurred in a patient with a parietal rim-enhancing mass on computed tomographic scan. at operation a brain abscess was identified and removed. peptostreptococcus and fusobacterium were isolated, possibly of dental origin. the possible sources of this intracranial bleeding are discussed. a neoplasm should not always be considered in the case of a cerebral ring-enhancing mass complicated with intracranial bleeding; in selected cases, brain abscess should be excluded t ...	1987	3502181
severe infections of the head and neck resulting from gas-forming organisms: report of case.	a case of severe infection of the head and neck associated with gas-forming organisms in a patient with fractures of the mandible is presented. selected aspects of antibiotic therapy are discussed.	1987	3468169
comparative serum bactericidal activity against test anaerobes in volunteers receiving imipenem, clindamycin, latamoxef and metronidazole.	ten healthy volunteers received on separate days the following regimens: imipenem 500 mg, clindamycin 600 mg, latamoxef 1 g, and metronidazole 500 mg. the antibiotics were given intravenously as an infusion over 15 min. blood samples were obtained before and 30 min, 1 and 6 h after the start of the infusion. serum bacteriostatic and bactericidal activities were measured against the following strains of strict anaerobes: two strains of bacteroides fragilis, one strain each of b. vulgatus, b. thet ...	1987	3471750
effects of extracellular products of a presumed gingival pathogen, the bacterium peptostreptococcus 84h14s, on cultured human fibroblasts and hela cells.	culture fluids from this microorganism, fibroblasts and hela cells contained potent factors which inhibited thymidine uptake in hela cells. the outcome of heating the culture fluid and fractionating it by gel filtration suggested that this was not due to bacterial hyaluronidase activity. purified peptostreptococcal hyaluronidase and several commercially-available mucopolysaccharidases also did not inhibit thymidine uptake.	1987	3478031
[peptostreptococcus sepsis complicating third molar pericoronitis].		1987	3483065
effect of streptococcus parvulus and peptostreptococcus magnus on cytotoxin levels of clostridium difficile in anaerobic continuous flow culture.	an anaerobic continuous flow (cf) culture method was used in order to study the effect of peptostreptococcus magnus and streptococcus parvulus, anaerobic gram-positive cocci which are members of intestinal bacterial flora, on growth and cytotoxin-activity of clostridium difficile. the growth- and the cytotoxin activity-patterns of c. difficile in an established cf culture of p. magnus were similar to those of c. difficile alone. on the other hand, in the mixed culture system of c. difficile and ...	1987	3431487
bacteria in bile of patients with bile duct inflammation.	bile samples taken intraoperatively from 100 patients with three different bile system diseases were subjected to bacteriological analysis. statistically significant differences between the types of aerobic and anaerobic bacteria present in the bile were found.	1987	3436318
[studies on antimicrobial concentrations of flomoxef in serum, pelvic dead space exudate, and pelvic organs/tissues].	to women undergoing radical and total hysterectomy, flomoxef (fmox, 6315-s) in a dose of 2 g was administered by intravenous drip infusion over 1 hour and drug concentrations in serum and pelvic dead space exudate as well as pelvic organs/tissues were determined over time. the following results were obtained: 1. serum concentrations of fmox after intravenous infusion showed the peak value of 92.86 +/- 17.05 micrograms/ml at the end of infusion and then gradually decreased to 29.00 +/- 10.49 micr ...	1987	3444023
anaerobic bacteraemia.		1987	3449455
the effect of carbon dioxide on the in vitro activity of erythromycin and ru-28965 against anaerobic bacteria.	the in vitro activity of erythromycin and ru-28965 (a novel macrolide antimicrobial with improved pharmacokinetics) was determined against a variety of anaerobic bacteria in anaerobic atmospheres with and without added carbon dioxide. minimum inhibitory concentrations (mic) were determined using an antimicrobial incorporation technique in wilkins-chalgren agar (oxoid, uk) containing saponinlysed horse blood to a final concentration of 10%. the inoculum used was approximately 10(4) colony forming ...	1987	3113907
in vitro activity of vancomycin and teicoplanin against anaerobic bacteria.	the in vitro activity of vancomycin and teicoplanin, a new glycopeptide antimicrobial, was determined against a total of 286 anaerobic bacteria including bacteroides fragilis group (100), b. melaninogenicus (21), b. bivius (16), fusobacterium spp. (15), peptococcus spp. (20), peptostreptococcus spp. (21), clostridium perfringens (23), c. difficile (41) and propionibacterium acnes (29). minimum inhibitory concentrations (mic) were determined using an antimicrobial incorporation technique in wilki ...	1987	2962846
[nonclostridial obligate anaerobes--the main causative agents of lung abscesses].	the use of the anaerobic technique in bacteriological investigations has made it possible to establish the leading role of asporogenous anaerobes in the etiological structure of the causative agents of pulmonary abscesses. asporogenous anaerobes have been isolated from 93% of all examined patients, and in 54.4% of such patients these microorganisms have proved to be an independent etiological factor. among asporogenous anaerobes, the main causative agents of pulmonary abscesses have been found t ...	1987	3296575
[revision of the taxonomy of the genera peptococcus and peptostreptococcus and a study on their identification system].		1987	3302346
[activity of fusidic acid on strictly anaerobic bacteria].	fusidic acid is a well known antimicrobial agent due to its narrow spectrum of activity against gram positive bacteria and especially staphylococci. therefore, it is after used preventively against bacterial infection in traumatology, but the susceptibility of anaerobic bacteria is not well known. we have studied, the in vitro activity of sodium fusidate against 147 strains of anaerobic bacteria. this antibiotic has a moderate activity against bacteroides, more significant against clostridium, p ...	1987	3302863
the growth of oral bacteria on saliva.	the present experiments were aimed at studying the degradation of salivary glycoproteins by the oral microflora. to this end, s. sanguis i strain ny476 and s. sanguis ii (s. mitior) strain ny581 were grown continuously in human-whole saliva. under these conditions, the strains produced a variety of cell-associated hydrolytic activities, including glycosidases, exo- and endopeptidases, and esterases. s. sanguis ii generally exhibited higher levels of enzyme activity than did s. sanguis i, in part ...	1987	3305628
direct and indirect pathogenicity of anaerobic bacteria in respiratory tract infections in children.	bacteroides sp (bacteroides melaninogenicus, bacteroides oralis, and bacteroides fragilis), peptostreptococci, and fusobacteria are important pathogens in respiratory tract infections (rti). these organisms are often recovered mixed with other aerobic, facultative, and anaerobic bacteria. evidence supporting their virulence is provided by studies showing their synergistic potentials in mixed infections and their increased virulence when encapsulated. a recent increase in numbers of beta-lactamas ...	1987	3318301
omental pedicle graft in the management of infected ascending aortic prostheses.	two patients had mediastinal infections with chronic draining sinus tracts that involved a vascular prosthesis in the ascending aorta. in 1 patient, a false mycotic aneurysm developed and in the other, a beginning rupture of the proximal suture line. in both patients, the infection was cured by replacing the infected aortic prosthesis combined with wrapping the new prosthesis with a pedicled omental graft. an omental graft was used to protect the vascular prosthesis and minimize the risk of recu ...	1987	3318738
proline requirement for glucose utilization by peptostreptococcus anaerobius atcc 27337.	resting cells of peptostreptococcus anaerobius maintained under anaerobic conditions were unable to metabolize either glucose or alanine. the addition of proline to the appropriate suspension, however, resulted in the immediate utilization of both compounds. fermentation of alanine by the cells required that stoichiometric concentrations of proline be present in the medium; and during the oxidation of alanine, proline was simultaneously reduced to the ring cleavage product delta-aminovaleric aci ...	1987	3804441
early microbial colonization of permucosal implants in edentulous patients.	in edentulous patients, the microbial colonization of permucosal implants of sintered hydroxyapatite was studied. samples were taken from mucosa and dentures before insertion of implants and from supra- and subgingival sites two to 10 weeks after insertion. in total, five patients and 10 implants with clinically healthy peri-implant tissues were studied. the samples were investigated by dark-field microscopy and anaerobic culture. the supragingival plaque of the implants was dominated by gram-po ...	1987	10872401
aerobic and anaerobic bacteriology of purulent nasopharyngitis in children.	cultures of aerobic and anaerobic bacteria were obtained from the inferior nasal meatus of 25 children with purulent nasopharyngitis and from 25 controls. microorganisms were isolated from all specimens. a total of 98 isolates (3.9 per patient), 45 aerobes (1.8 per patient) and 53 anaerobes (2.1 per patient), were isolated in patients with purulent nasopharyngitis. seventy-three isolates (2.9 per patient), 47 aerobes (1.9 per patient) and 26 anaerobes (1.0 per patient) were found in the controls ...	1988	3356794
identification of gram-positive anaerobic cocci by use of systems for detecting pre-formed enzymes.	two systems for detecting pre-formed enzymes, rapid ana and a prototype system from api, were compared in a blind study for their ability to identify 69 gram-positive anaerobic cocci isolated from clinical specimens. both systems were able to identify peptostreptococcus anaerobius, ps. asaccharolyticus and ps. micros accurately without the need for further tests. the prototype api system identified all isolates of ps. magnus correctly, but the rapid ana system misidentified several isolates as p ...	1988	3357195
metabolism of sennosides by human intestinal bacteria.	during the course of studies on the metabolism of sennosides by human intestinal bacteria, an enzyme which takes part in the reduction of sennosides and sennidins was originally isolated from peptostreptococcus intermedius. this enzyme catalyzed the electron transfer from nadh to fad, fmn or benzyl viologen, which reduced nonenzymatically sennosides and sennidins to 8-glucosyl-rhein anthrone and rhein anthrone, respectively.	1988	3368516
enrichment and isolation of a ruminal bacterium with a very high specific activity of ammonia production.	when mixed ruminal bacteria were inoculated into semicontinuous cultures (25% transfer every other day) containing lactate, dulcitol, pectin, or xylose and trypticase (1 g/liter) as the sole nitrogen source, the specific activity of ammonia production increased. the greatest enrichment was observed with lactate and xylose, and in these cases the specific rate of ammonia production was eightfold higher than that of the ruminal fluid control (approximately 35 nmol of ammonia per mg of protein per ...	1988	3377500
recovery of anaerobic bacteria from clinical specimens in 12 years at two military hospitals.	examination of 15,844 clinical specimens submitted over 12 years (1973 to 1985) to the anaerobic microbiology laboratories in two military hospitals demonstrated the recovery of anaerobic bacteria in 4,458 (28.1%) specimens. the specimens yielded 6,557 anaerobic isolates (1.47 isolates per specimen). bacteroides spp. accounted for 43% of all isolates; anaerobic gram-positive cocci, 26%; clostridium spp., 7%; and fusobacterium spp., 4%. bacteroides spp. predominated in abscesses, obstetrical and ...	1988	3384929
cecal and fecal bacterial flora of the mongolian gerbil and the chinchilla.	the mongolian gerbil is being increasingly used as a laboratory animal and as a pet. both chinchillas and gerbils are used as animal models for otitis media and other otic research. previously, only incomplete information was available regarding the indigenous bacterial flora of the lower intestinal tracts of these coprophagic animals. using the strict anaerobic methodology of the virginia polytechnic institute anaerobe laboratory, we studied the predominant bacterial flora of the cecum and feca ...	1988	3389814
presentation and management of neck abscess: a retrospective analysis.	a retrospective analysis of deep neck abscesses managed at the los angeles county-usc medical center was performed. fifty-one patients met the criteria and were reviewed as to presentation, etiology, location, and microbiology. hemolytic streptococci and anaerobic species, especially bacteroides and peptostreptococci, were the most common organisms isolated. intravenous drug abuse was the most common etiology, and was an important source of primary carotid space infection. this group was most co ...	1988	3398666
induction of chronic arthritis in rats by cell wall fragments of anaerobic coccoid rods isolated from the faecal flora of patients with crohn's disease.	crohn's disease and ulcerative colitis are accompanied by seronegative arthritis in about one fifth of the cases. in the present study, cell wall fragments from major residents such as eubacterium, coprococcus and peptostreptococcus species, isolated from the faecal flora of patients with crohn's disease, were tested for properties to induce chronic arthritis in lewis rats. cell wall fragments from eubacterium contortum strains me44 and me47 were found to induce chronic arthritis; peptostreptoco ...	1988	3410168
efficacy and safety of single-dose ceftizoxime vs. multiple-dose cefoxitin in preventing infection after vaginal hysterectomy.	the efficacy, safety and effect of a single, 1-g dose of ceftizoxime on the return of vaginal flora were compared to those of the standard regimen of three 2-g doses of cefoxitin for prophylaxis in 99 randomized women undergoing vaginal hysterectomy. thirty-four were studied in dallas and 65 in los angeles. surgical procedures were comparable for all the antibiotic groups, although proportionately more simple hysterectomies were performed in dallas. the patients received a povidone-iodine vagina ...	1988	3422693
fermentation of peptides and amino acids by a monensin-sensitive ruminal peptostreptococcus.	a monensin-sensitive ruminal peptostreptococcus was able to grow rapidly (growth rate of 0.5/h) on an enzymatic hydrolysate of casein, but less than 23% of the amino acid nitrogen was ever utilized. when an acid hydrolysate was substituted for the enzymatic digest, more than 31% of the nitrogen was converted to ammonia and cell protein. coculture experiments and synergisms with peptide-degrading strains of bacteroides ruminicola and streptococcus bovis indicated that the peptostreptococcus was u ...	1988	2975156
protein l. a novel bacterial cell wall protein with affinity for ig l chains.	a novel ig-binding protein has been isolated from the surface of bacteria belonging to the anaerobic species peptococcus magnus. to solubilize the protein, peptococci were treated with different proteolytic enzymes (papain, pepsin, and trypsin) or with mutanolysin, a bacteriolytic agent known to digest the cell walls of streptococci. papain, trypsin, and mutanolysin all solubilized peptides showing affinity for radiolabeled human igg in western blot analysis. compared with papain and trypsin, mu ...	1988	3125250
characterization of cross-reactive proteins detected by culturette brand rapid latex test for clostridium difficile.	clostridium sporogenes, peptostreptococcus anaerobius, and bacteroides asaccharolyticus have been reported to react in the culturette brand rapid latex test (marion scientific, div. marion laboratories, inc., kansas city, mo.) for clostridium difficile. from the results of this study we showed that c. sporogenes and p. anaerobius produce a protein which is very similar biochemically and immunologically to the protein of c. difficile that is detected by the test. thus, the positive latex reaction ...	1988	3128574
in vitro activities of daptomycin (ly146032) and paldimycin (u-70,138f) against anaerobic gram-positive bacteria.	the in vitro activities of daptomycin (ly146032), paldimycin (u-70,138f), vancomycin, and penicillin g against 344 clinical isolates of anaerobic gram-positive bacteria were determined by an agar dilution method in calcium-supplemented (50 micrograms/ml) wilkins-chalgren medium, using an inoculum of 10(5) cfu. daptomycin demonstrated excellent activity against a broad range of anaerobic gram-positive cocci and bacilli, including peptostreptococcus, eubacterium, bifidobacterium, actinomyces, prop ...	1988	2840019
[pharmacokinetic, bacteriological and clinical studies of cefuzonam in the field of obstetrics and gynecology. study group of cefuzonam in the field of obstetrics and gynecological infections].	a multi-center open study was conducted to investigate cefuzonam (czon, l-105) regarding to its pharmacokinetic, bacteriological and clinical aspects in the field of obstetrics and gynecology with the participation of 31 medical institutions and the related facilities. the results are summarized as follows. 1. peak mics of czon for staphylococcus aureus, coagulase (-) staphylococci, escherichia coli, klebsiella pneumoniae, bacteroides fragilis group, peptostreptococcus spp. isolated from obstetr ...	1988	3050195
peptostreptococcus magnus septic arthritis--a report and review of the english literature.	we describe a 30-year-old female presenting with synovitis of the left 4th proximal interphalangeal joint. anaerobic cultures of the synovial membrane grew peptostreptococcus magnus. the synovitis resolved after a course of intravenous penicillin g. the role of the anaerobic bacterium peptostreptococcus magnus in joint infections is reviewed.	1988	3060615
anaerobic infections in the head and neck region.	anaerobic bacteria form the predominant flora of the oral cavity, outnumbering facultative organisms by 10-1,000: 1. the type of anaerobic bacteria and their concentration depend on the anatomical site and the degree of anaerobiosis in the different sites in the mouth. three groups of anaerobic bacteria inhabit the oral cavity; the strict anaerobes, the moderate anaerobes, and the microaerophilic group of organisms. the majority of anaerobic bacterial infections occurring in the region of the mo ...	1988	3074469
brain abscess after dilatation of esophageal stricture.	two children, below 15 years of age, developed brain abscesses after esophageal dilation. they were among 40 children treated for supratentorial brain abscesses during the past 10 years. pus culture of one of the abscesses was peptostreptococcus, which is a common oropharyngeal flora. clinicians should be aware of this complication since the prognosis is usually satisfactory after prompt treatment.	1988	3077165
peptostreptococcus micros in polymicrobial abscesses.		1988	2894531
the in-vitro activity of three long-acting cephalosporins against bacteroides fragilis, peptostreptococcus species and clostridium perfringens.	the in-vitro activity of three long-acting cephalosporins (cefotetan, cefonicid and ceftriaxone) was compared against 206 clinical isolates of bacteroides fragilis, peptostreptococcus species and clostridium perfringens, using an agar dilution procedure to determine minimum inhibitory concentrations (mics). clindamycin was included as a comparator. cefotetan was much more active than the two other cephalosporins against strains of bacteroides fragilis (mic90 16 mg/l compared with greater than 12 ...	1988	2902937
semiquantitative culture results and pathogenic significance of obligate anaerobes in peritonsillar abscesses.	we studied the bacteria in consecutive peritonsillar abscesses using semiquantitation of the primary culture findings and correlated the results to clinical parameters. puncture-aspirated pus from 42 abscesses yielded 133 isolates. group a streptococci were isolated 10 times and, unlike other bacteria, were isolated 4 times in pure culture; other beta-hemolytic streptococci were found in 8 abscesses, and anaerobes were found in 28. the infections were polymicrobial, with two to seven bacteria in ...	1988	3164314
preformed enzyme profiles of reference strains of gram-positive anaerobic cocci.	the preformed (constitutive) enzyme profiles of 30 type strains and reference strains of gram-positive anaerobic cocci were determined with two commercial systems, rapid ana and a prototype system from api. both systems identified peptostreptococcus anaerobius, ps. asaccharolyticus, ps. indolicus, ps. magnus and ps. micros accurately, except for one strain of ps. magnus misidentified as ps. micros by the rapid ana system. the indole-negative, butyrate-producing cocci (classified at present as ps ...	1988	3172172
brain abscess from chronic odontogenic cause: report of case.	a case of a chronic odontogenic abscess that probably precipitated a frontopatrietal brain abscess by hematogenous spread has been presented. a thorough examination of the patient showed no other source of infection. peptostreptococcus sp was found as the common pathogen despite 6 days of broad-spectrum antibiotic therapy. this case emphasizes the important role of dentistry in medical diagnosis and treatment implemented in the hospital setting.	1988	3183246
comparative activities of cefuroxime, amoxicillin-clavulanic acid, ciprofloxacin, enoxacin, and ofloxacin against aerobic and anaerobic bacteria isolated from bite wounds.	we studied the comparative in vitro activities of 10 oral antimicrobial agents against 147 aerobic and 61 anaerobic bacteria making up species in 13 genera (staphylococcus aureus, streptococci, eikenella corrodens, pasteurella multocida, haemophilus-actinobacillus spp., m-5, ef-4, moraxella spp., flavobacterium iib, bacteroides melaninogenicus, bacteroides spp., fusobacterium spp., and peptostreptococcus spp.) that were isolated from bite wounds. cefuroxime was generally greater than fourfold mo ...	1988	3190202
tetracycline resistance and tetm in oral anaerobic bacteria and neisseria perflava-n. sicca.	tetracycline-resistant organisms isolated from six patients with periodontal disease included bacteroides spp., eubacterium spp., fusobacterium nucleatum, neisseria perflava-n. sicca, peptostreptococcus anaerobius, veillonella parvula, and facultative streptococci. all but the bacteroides spp. and eubacterium spp. hybridized with the tetm determinant. an additional 417 bacterial strains were screened, and 4% of both the oral streptococci and the fusobacterium spp. hybridized with the tetm probe.	1988	3190205
infection of a prosthetic knee joint with peptostreptococcus magnus.	infection of a prosthetic knee joint with peptostreptococcus magnus in an immunosuppressed patient with rheumatoid arthritis is described. the organism is a skin commensal, generally thought to be of low pathogenicity; the difficulty in making the diagnosis is emphasised.	1988	3196085
presence of anaerobic bacteria in conjunctivitis associated with wearing contact lenses.	ten anaerobic bacterial species were found in conjunctival cultures obtained from six patients who wore contact lenses and developed conjunctivitis. in three instances the anaerobic bacteria were associated with facultative bacteria. the predominant anaerobic isolates were peptostreptococcus species (three isolates), bacteroides sp. (two), and fusobacterium sp. (two). it was the practice of five of the six patients to wet their lenses with saliva. it is recommended that a culture for anaerobes b ...	1988	3207314
empyema caused by kingella denitrificans and peptostreptococcus spp. in a patient with bronchogenic carcinoma.	empyema caused by kingella denitrificans and peptostreptococcus spp. was diagnosed in a patient with bronchogenic carcinoma. this appears to be the third report providing evidence of a pathogenic role for k. denitrificans, and the first concerning infection in the pleural space and in a patient with underlying immunosuppressive disease. k. denitrificans should be added to the list of fastidious gram-negative bacteria associated with opportunistic infections in the compromised host.	1988	3229487
central nervous system infection associated with anaerobic bacteria in two dogs and two cats.	central nervous system (cns) infection caused by anaerobic bacteria (including bacteroides, fusobacterium, peptostreptococcus, and eubacterium) was diagnosed in two dogs and two cats. in one dog there was extensive meningomyeloencephalitis, presumably the result of hematogenous spread of bacteria from lung abscesses and bacterial endocarditis. subdural empyema of unknown origin was found in a second dog and two cats. clinical signs in all four animals included mental depression and focal neurolo ...	1988	3230556
evaluation of a commercial latex test for clostridium difficile for reactivity with c. difficile and cross-reactions with other bacteria.	seventy-eight species of bacteria (739 isolates) were tested for reactivity with a commercial latex test for clostridium difficile. all noncytotoxic as well as cytotoxic strains of c. difficile reacted positively. immuno-specific cross-reactions were found only with c. sporogenes, proteolytic c. botulinum, and peptostreptococcus anaerobius.	1988	3235677
bacteriology of periodontal disease in the cat.	subgingival plaque samples were obtained from 32 cats showing different stages of periodontal disease. correlations were sought between gingival index scores and the prevalence of various microbial groups, and between microbial populations found in sites designated as most-affected and least-affected within individual cats. the tendency with higher gingival index scores, and with the most-affected sites, was toward a microbial population composed to a greater extent of anaerobic gram-negative ro ...	1988	3245794
clinical and laboratory findings in women with bacterial vaginosis and trichomoniasis versus controls.	we report comprehensively on the clinical and laboratory findings in 30 women with bacterial vaginosis (bv), 30 with vaginal trichomoniasis and 30 with normal secretions. women with trichomoniasis were more often divorced (chi 2 test, p much less than 0.001), more often complained of dyspareunia (chi 2 test, p less than 0.05), frequently had discharge present in the vaginal vestibule, and showed one or more signs of vaginitis in half the cases. a 'moth-eaten' cervix was seen in only four women ( ...	1988	3260566
[aerobic and anaerobic bacterial flora of crural ulcers].	in a group of 600 patients treated in the metropolitan dermatological hospital in warsaw bacteriological examination were carried out of swabs from the untreated crural ulcers. in 95% of these cultures growth of pathological aerobic organisms was obtained. coagulase-positive staphylococci (st. aureus) and gram-negative bacteria (pseudomonas aeruginosa, proteus vulgaris, enterobacter sp and e. coli) prevailed. in 27% of cases the cultured strains were resistant to the generally available antibiot ...	1988	3268941
[microbiology of samples from patients with periodontal pockets].	a microbiologic study was carried out in 39 patients with periodontal affections, who were surgically treated. a formulary was drawn up for the collection of the following data: age, sex, criteria of russell periodontal index and microbiologic results. the contents of the periodontal pocket was collected at the time of the surgical procedure, and settled in assay tubes with culture medium to provide bacterial growth. the highest percentage of microorganisms were those of genus peptostreptococci, ...	1988	3272438
a multicenter comparative study of cefotetan once daily and cefoxitin thrice daily for the treatment of infections of the skin and superficial soft tissue.	to compare the effectiveness of cefotetan administered at 2 g once a day with cefoxitin at 1 or 2 g three times a day in the treatment of hospitalized patients with skin and superficial soft tissue infections, 194 patients from eight centers were enrolled in an open, randomized trial. most of the 104 evaluable patients in the cefotetan group and 50 in the cefoxitin group were young men with community-acquired, moderate or severe cellulitis, or abscesses of the upper and lower extremities caused ...	1988	3287976
microbiological and clinical aspects of aspiration pneumonia.	aspiration pneumonia is characterized by a pneumonitis in a dependent segment of the lung with typical necrosis or abscess-formation in the parenchyma. observed aspiration or predisposition to aspiration, cavitation or abscess formation, with or without empyema fluid and isolation of distinctive micro-organisms are important clues to the diagnosis. diagnostic procedures to collect anaerobic uncontaminated secretions are transtracheal aspiration, blood cultures, pleural fluid aspiration, fibreopt ...	1988	3290185
peptostreptococcus magnus does not cause hematogenous infections in experimental arthroplasties.	rabbits with cemented endoprostheses in the knee joints were given 1-ml intravenous injections of suspensions containing peptostreptococcus magnus (3 x 10(8) colony-forming units/ml) on three consecutive days seven weeks postoperatively. the animals were killed after another seven weeks. the bacterial challenge did not result in infection. this is in contrast to a previous study using the same experimental system with another anaerobic bacterial species, propionibacterium acnes, which caused inf ...	1989	2924473
studies on mixed populations of human intestinal bacteria grown in single-stage and multistage continuous culture systems.	mixed intestinal bacteria were grown for 336 h in two identical single-stage chemostats at low growth rates in a carbohydrate-limited medium. complex bacterial populations were maintained and anaerobes always outnumbered aerobes. the predominant organisms belonged to the genera bacteroides, bifidobacterium, lactobacillus, clostridium, eubacterium, propionbacterium, peptococcus, and peptostreptococcus. bacteroides species predominated in both fermentors, particularly b. ovatus and b. thetaiotaomi ...	1989	2930171
protein l: an immunoglobulin light chain-binding bacterial protein. characterization of binding and physicochemical properties.	protein l, a cell wall molecule of the bacterial species peptostreptococcus magnus with affinity for immunoglobulin (ig) light chains, was isolated after solubilization of the bacterial cell walls with mutanolysin or from the culture medium by a single affinity chromatography step on human igg-sepharose. a major protein band with an apparent molecular weight of 95,000 was obtained from both sources. the protein from the growth medium was size heterogeneous. from 1 ml of packed bacteria was prepa ...	1989	2479638
application of chemotaxonomic techniques to the taxonomy of anaerobic bacteria.	the use of chemical characters in bacterial classification and identification has proved to be an essential component of modern systematics. several clinically important anaerobic genera, such as bacteroides, clostridium, eubacterium, fusobacterium and peptostreptococcus, are known to be heterogeneous of the basis of chemotaxonomic and genetic data and are in need of further examination. recent work on bacteriodes has led to the genus being redefined and restricted to species within the former b ...	1989	2479974
[isolation of anaerobes during a 30-month observation at a hospital microbiology laboratory].	the authors evaluate retrospectively the results obtained from the research of anaerobial bacteria on 1313 samples received at the microbiology laboratory of the "ospedale civile di ivrea" over a period of 31 months (6/1/86-12/31/88). from this evaluation, high percentages of detection of anaerobic bacteria are emerging in the following infections: appendiculare abscesses (60%), intestinal operations (71%), wounds (57%), tubovarian abscesses (100%), as well as thoracic empyema (50%). also releva ...	1989	2490397
gaspak plus versus anaerocult a--two carbon dioxide/hydrogen systems for cultivation of anaerobes.	two disposable carbon dioxide/hydrogen gas-generating systems (gaspak plus and anaerocult a) were compared by assessing growth of obligate anaerobic bacteria. eighty strains representing 28 species of anaerobic bacteria commonly occurring at various body sites were seeded onto 4 brain heart chocolate agar plates using a spiral plater; and 1 plate each was subsequently incubated in 2 anaerocult a and 2 gaspak plus systems. bacterial growth was expressed as colony-forming units per ml (cfu/ml), re ...	1989	2496538
sodium dependent acetate formation from co2 in peptostreptococcus products (strain marburg).	washed cells of peptostreptococcus products (strain marburg), which were incubated in the presence of co/co2/n2 (50%/17%/33%; 200 kpa) catalyzed the synthesis of acetate from carbon monoxide. the rate of acetate formation from co was stimulated more than threefold by the addition of sodium (10 mm); potassium did not effect acetate synthesis. the degree of stimulation was dependent on the sodium concentration; the dependence followed simple michaelis-menten kinetics. the apparent km for sodium wa ...	1989	2498155
coumamidines, new broad spectrum antibiotics of the cinodine type. iii. microbiologic activity of coumamidine gamma 1.	the coumamidines are novel antibiotics with activity against a wide spectrum of aerobic gram-positive and gram-negative bacteria. all microbiological studies were performed on coumamidine gamma 1. the mic90s (micrograms/ml) of coumamidine are as follows: staphylococcus aureus 1.0, streptococcus pyogenes 8, enterobacteriaceae 2.0, pseudomonas aeruginosa 8, campylobacter jejuni and campylobacter coli 1, legionella pneumophila 8, haemophilus influenzae 0.5, neisseria gonorrhoeae 0.5. coumamidine ha ...	1989	2498268
the active species of "co2" formed by carbon monoxide dehydrogenase from peptostreptococcus productus.	carbon monoxide dehydrogenase of anaerobic bacteria catalyzes the reversible conversion of co to "co2". with the enzyme from peptostreptococcus productus it is shown that co2 rather than hco3- (h2co3) is the active species of "co2" formed by this dehydrogenase.	1989	2504176
bacteriotherapy for chronic relapsing clostridium difficile diarrhoea in six patients.	six patients with chronic relapsing diarrhoea caused by clostridium difficile were treated with rectal instillation of homologous faeces (one patient) or a mixture of ten different facultatively aerobic and anaerobic bacteria diluted in sterile saline (five patients). the mixture led to a prompt loss of cl difficile and its toxin from the stools and to bowel colonisation by bacteroides sp, which had not been present in pre-treatment stool samples. strains of escherichia coli, cl bifermentans, an ...	1989	2566734
epidural abscess and subdural empyema.	epidural abscess and subdural empyema are serious intracranial infections that result in significant morbidity and mortality. frequently, they are secondary to sinusitis or middle ear disease, and the bacteria involved are inhabitants of the upper respiratory tract. symptoms may be mild and mimic the symptoms of the underlying infection. however, especially with subdural empyema, alteration in the level of consciousness and focal neurologic deficits are common. morbidity and mortality are minimi ...	1989	2568981
comparison of four commercial brucella agar media for growth of anaerobic organisms.	four different commercial brucella blood agar plating media (anaerobe systems, bbl microbiology systems, remel, and scott laboratories) were compared for the abilities to recover anaerobic organisms from clinical specimens and to support the growth of american type culture collection anaerobic stock cultures. following 24 h of incubation in an anaerobe chamber, anaerobe systems prereduced, anaerobically sterilized brucella plates yielded 63% of the total clinical anaerobe isolates, the scott med ...	1989	2584378
microbiology of infected epidermal cysts.	specimens from 231 epidermal cyst abscesses were inoculated on media supportive for growth of aerobic and anaerobic bacteria. of these, 192 yielded bacterial growth. aerobic or facultative bacteria only were recovered in 84 specimens (44%), anaerobic bacteria only in 57 specimens (30%), and mixed aerobic and anaerobic bacteria in 51 specimens (26%). a total of 315 isolates (162 anaerobes and 153 aerobes) were recovered. an average of 0.8 aerobic or facultative isolates per infected cyst were rec ...	1989	2589860
josamycin concentrations in human dental granuloma after a single oral administration of josamycin.	1. josamycin concentrations in human serum and dental granuloma after a single oral administration of josamycin (600 mg) were assayed by an agar diffusion (paper disc) method. 2. the mean peak josamycin concentrations in serum and dental granuloma occurred at an identical time, approximately 90 min, and were 0.88 micrograms/ml and 1.61 micrograms/g, respectively. 3. the mean concentration ratio of dental granuloma to serum at the peak time was 2.24. 4. josamycin concentration in dental granuloma ...	1989	2591712
studies on microaerophilic cocci (stuart-schwan cocci) isolated from summer mastitis and other pyogenic infections of cattle.	forty-nine strains of microaerophilic gram-positive cocci (stuart-schwan cocci) isolated from summer mastitis, "pyogenes"-mastitis, other pyogenic conditions of danish cattle and swine, and from the sheep headfly hydrotaea irritans were biochemically characterized with the api 50 ch and api zym test kit systems, and screened for production of a variety of extracellular enzymes by agar plate methods. for comparison 4 strains isolated from swedish cases of heifer and dry cow mastitis were included ...	1989	2596384
plasmid analysis and antimicrobial susceptibilities of peptostreptococcus species.	there are no published methods on plasmid isolation from peptostreptococcus spp., therefore two methods of plasmid isolation from this genera were analysed: the boiling and alkaline-sds methods. plasmid dna was not recovered by the boiling method, however, with the alkaline-sds method, cryptic plasmid dna was detected in two p. asaccharolyticus and one p. magnus strains. to achieve optimum lysis, peptostreptococcus cells were treated with lysozyme (2 mg/ml) for 15 min. at 37 degrees c followed b ...	1989	2599360