Publications

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[inhibition of ribulosediphosphate carboxylase by adenosine triphosphate in autotrophic organisms]. 19734198598
a comparison of the unfolding and dissociation of the large ribosome subunits from rhodopseudomonas spheroides n.c.i.b. 8253 and escherichia coli m.r.e. 600.1. the behaviour of the large ribosomal subunit from rhodopseudomonas spheroides (45s) has been compared with the 50s ribosome from escherichia coli m.r.e. 600 (and e. coli m.r.e. 162) during unfolding by removal of mg(2+) and detachment of ribosomal proteins by high univalent cation concentrations. the extent to which these processes are reversible with these ribosomes has also been examined. 2. the r. spheroides 45s ribosome unfolds relatively slowly but then gives rise directly to two ribonuc ...19734201305
control of magnesium-protoporphyrin chelatase activity in rhodopseudomonas spheroides. role of light, oxygen, and electron and energy transfer.1. magnesium-protoporphyrin chelatase activity, previously shown in whole cells of rhodopseudomonas spheroides, could not be demonstrated in cell-free extracts prepared in different ways, although spheroplasts retained moderate activity. slight activity was detected also in whole cells of rhodospirillum rubrum. 2. the effects on the activity of the enzyme of inhibitors of electron and energy transfer were studied in whole cells of rps. spheroides. amytal, rotenone, azide and cyanide inhibited at ...19734202754
comparison of phototrap complexes from chromatophores of rhodospirillum rubrum, rhodopseudomonas spheroides, and the r-26 mutant of rhodopseudomonas spheroides. 19734204253
studies on ion transport in cells of photosynthetic bacteria. 3. the influence of uncouplers on hydrogen ion change. 19734205460
[comparative study of nitrogen fixation in purple bacteria]. 19734205730
the function and localization of ubiquinone in the nadh and succinate oxidase systems of rhodopseudomonas palustris. 19734147456
energy transduction in photosynthetic bacteria. vi. respiratory sites of energy conservation in membranes from dark-grown cells of rhodopseudomonas capsulata. 19734148029
purification and some properties of a protein factor required for light-dependent transhydrogenase in rhodopseudomonas spheroides. 19734150340
isocitrate dehydrogenase from rhodopseudomonas spheroides: kinetic mechanism and further characterization. 19734150342
are both fractions of rhodopseudomonas spheroides y ala synthetase isoenzymes? 19734364574
anomalous ligand binding by a class of high spin c-type cytochromes. 19734346352
water and cytochrome oxidation-reduction reactions. 19734349915
primary events in the photosynthetic reaction centre from rhodopseudomonas spheroides strain r26: triplet and oxidized states of bacteriochlorophyll and the identification of the primary electron acceptor. 19734350260
photochemical systems of rhodospirillum rubrum. light-induced reactions and biological functions of c-type cytochromes in relation to p-870. 19734351207
genetic mutations affecting the respiratory electron-transport system of the photosynthetic bacterium rhodopseudomonas capsulata.alternative energy-converting systems permit the nonsulfur purple photosynthetic bacterium rhodopseudomonas capsulata to grow either with light or (dark) respiration as the source of energy. respiratory mutants, unable to grow aerobically in darkness, can be readily isolated and the defective step(s) in their respiratory mechanisms can be identified by study of biochemical activities in membrane fragments derived from photosynthetically grown cells. such analysis of appropriate mutants and rever ...19734351385
d-3-hydroxybutyrate dehydrogenase from rhodopseudomonas spheroides. kinetic mechanism from steady-state kinetics of the reaction catalysed by the enzyme in solution and covalently attached to diethylaminoethylcellulose.1. the reversible nad(+)-linked oxidation of d-3-hydroxybutyrate to acetoacetate in 0.1m-sodium pyrophosphate buffer, ph8.5, at 25.0 degrees c, catalysed by d-3-hydroxybutyrate dehydrogenase (d-3-hydroxybutyrate-nad(+) oxidoreductase, ec 1.1.1.30), was studied by initial-velocity, dead-end inhibition and product-inhibition analysis. 2. the reactions were carried out on (a) the soluble enzyme from rhodopseudomonas spheroides and (b) an insoluble derivative of this enzyme prepared by its covalent ...19734352835
d-3-hydroxybutyrate dehydrogenase from rhodopseudomonas spheroides. kinetics of radioisotope redistribution at chemical equilibrium catalysed by the enzyme in solutions.1. the reversible nad(+)-linked oxidation of d-3-hydroxybutyrate to acetoacetate in 0.1m-sodium pyrophosphate buffer, ph8.5, at 25.0 degrees c, catalysed by d-3-hydroxybutyrate dehydrogenase (d-3-hydroxybutyrate-nad(+) oxidoreductase, ec 1.1.1.30), was studied kinetically at chemical equilibrium by monitoring radioisotope redistribution with sodium dl-hydroxy[3-(14)c]butyrate and [4-(3)h]nad(+)(labelled in the nicotinamide ring). 2. when all substrates are maintained at concentrations approachin ...19734352836
oxidative phosphorylation and effects of aerobic conditions on rhodopseudomonas viridis. 19734354792
pigment content and molar extinction coefficients of photochemical reaction centers from rhodopseudomonas spheroides. 19734354794
light induced h+ uptake catalysed by photochemical reaction centres from rhodopseudomonas spheroides r26. 19734355316
model discrimination and nonlinear parameter estimation in the analysis of the mechanism of action of beta-hydroxybutyrate dehydrogenase from rhodopseudomonas spheroides. 19734356305
direct measurement of the midpoint potential of the primary electron acceptor in rhodopseudomonas spheroides in situ and in the isolated state: some relationships with ph and o-phenanthroline. 19734356786
the kinetic and redox potentiometric resolution of the carotenoid shifts in rhodopseudomonas spheroides chromatophores: their relationship to electric field alterations in electron transport and energy coupling. 19734358810
5-aminolevulinic-acid synthetase of rhodopseudomonas spheroides y. kinetic mechanism and inhibition by atp. 19734359141
enzymes of serine biosynthesis in rhodopseudomonas capsulata. 19734361552
spectral properties of reaction center preparations from rhodopseudomonas spheroides. 19734540710
kinetic and thermodynamic properties of membrane-bound cytochromes of aerobically and photosynthetically grown rhodopseudomonas spheroides. 19734540949
extraction of oxidized bacteriochlorophyll from illuminated photosynthetic reaction center particles. 19734540950
membrane proteins of rhodopseudomonas spheroides. 3. isolation, purification, and characterization of cell envelope proteins. 19734541219
membrane proteins of rhodopseudomonas spheroides. v. additional chemical characterization of a pigment-lipid-associated protein isolated from chromatophores. 19734541220
coupling between bacteriochlorophyll and membrane protein synthesis in rhodopseudomonas spheroides.bacteriochlorophyll-containing membranes from rhodopseudomonas spheroides contain proteins with estimated molecular weights of 26,000, 22,000, 19,000, and 10,000-6,000 (proteins 9, 10, 11, and 15) when analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. proteins 9, 10, and 11 may be associated with the reaction center form of bacteriochlorophyll and protein 15, with the light-harvesting form. these proteins were not detected in membranes from nonpigmented wild-type cells grown ...19734541415
the effect of redox potential on the coupling between rapid hydrogen-ion binding and electron transport in chromatophores from rhodopseudomonas spheroides. 19734541536
biosynthesis of spheroidene and hydroxyspheroidene in rhodopseudomonas species: experiments with nicotine as inhibitor.neurosporene replaces spheroidene and hydroxyspheroidene as the main carotenoid in rhodopseudomonas spheroides and rhodopseudomonas gelatinosa grown in the presence of nicotine. on removal of the nicotine, spheroidene and hydroxyspheroidene are formed at the expense of the accumulated neurosporene. this shows that nicotine inhibits introduction of the c-1 tertiary hydroxyl groups, and supports the postulated pathway neurosporene-->spheroidene-->hydroxyspheroidene.19734541982
recent studies of carotenoid biosynthesis in bacteria. 19734542022
-aminolevulinic acid dehydratase of rhodopseudomonas capsulata. 19734542319
dependency on environmental redox potential of photophosphorylation in rhodopseudomonas spheroides. 19734542403
polypyrroles formed from porphobilinogen and amines by uroporphyrinogen synthetase of rhodopseudomonas spheroides.1. uroporphyrinogen i synthetase of rhodopseudomonas spheroides was purified more than 200-fold from the soluble protein of broken bacterial cells. the enzyme had molecular weight 36000, an isoelectric point of 4.46 and migrated as a single active protein band on disc-gel electrophoresis at ph7.5 and 8.9. 2. the enzyme consumed porphobilinogen and formed uroporphyrinogen at ph8.2 without the accumulation of intermediates. in the presence of hydroxylamine, ammonia or methoxyamine the production o ...19734542566
fluorescence excitation spectra and the relative numbers of pigment molecules in reaction centres from rhodopseudomonas spheroides. 19734542616
mechanism and stereochemistry of the 5-aminolaevulinate synthetase reaction.1. two mechanisms for the biosynthesis of 5-aminolaevulinate from glycine and succinyl-coa (3-carboxypropionyl-coa) are considered. one of the mechanisms involves the retention of both the c-2 h atoms of glycine during the synthesis of 5-aminolaevulinate, whereas the other predicts the retention of only one of the c-2 h atoms of glycine. 2. highly purified 5-aminolaevulinate synthetase from rhodopseudomonas spheroides was used to show that the c-2 h atom of glycine with r configuration is specif ...19734543543
[intracellular distribution of vitamin b 12 in a culture of rhodopseudomonas spheroides]. 19734543823
5-aminolevulinic-acid synthetase of rhodopseudomonas spheroides y. purification and some properties. 19734543959
carotenoid biosynthesis in rhodopseudomonas spheroides. s-adenosylmethionine as the methylating agent in the biosynthesis of spheroidene and spheroidenone.[methyl-(14)c]methionine and s-adenosyl[methyl-(14)c]methionine were incorporated into the methoxycarotenoids spheroidene and spheroidenone by rhodopseudomonas spheroides. the incorporation was greatly enhanced in the presence of lysozyme. on degradation of labelled spheroidene by hydriodic acid, the (14)c label was recovered in methyl iodide. degradation of spheroidenone by reduction and allylic dehydration and demethylation of the reduction product gave a mixture of unlabelled carotenoid hydro ...19734544066
requirement of protein synthesis for maturation of ribosomal rna in rhodopseudomonas spheroides. 19734544261
control of 5-aminolaevulinate synthetase activity in rhodopseudomonas spheroides. the involvement of sulphur metabolism.1. the ;initial' 5-aminolaevulinate synthetase activity, that is the activity observed immediately after cell disruption, in extracts prepared from unharvested semianaerobically grown rhodopseudomonas spheroides, was twice that observed under the same assay conditions in extracts prepared from harvested cells. 2. the effect of oxygenation of a culture on the ;maximum' aminolaevulinate synthetase activity, that is the activity observed 1h after disruption of harvested cells, is markedly influence ...19734544404
control of 5-aminolaevulinate synthetase activity in rhodopseudomonas spheroides. the purification and properties of an endogenous activator of the enzyme.1. a low-molecular-weight activator of 5-aminolaevulinate synthetase was detected in extracts of rhodopseudomonas spheroides. the compound activates the enzyme extracted from oxygenated semi-anaerobically grown organisms by a factor of 6-8. 2. the activator was extensively purified, but owing to the exceedingly small amounts that could be extracted in the active form its structure was not determined. 3. the activator contains an acetylatable amino group; it is more stable at acid than at alkalin ...19734544405
[tricarboxylic acid cycle enzymes in various species of phototrophic bacteria]. 19734544539
purification and properties of uroporphyrinogen i synthetase from rhodopseudomonas spheroides. 19734539746
on the determination of the transmembrane ph difference in bacterial chromatophores using 9-aminoacridine. 19744434984
a simple method for extraction and partial purification of chlorophyll from plant material, using dioxane. 19744436293
bioenergetic aspects of bacteriophage replication in the photosynthetic bacterium rhodopseudomonas capsulata. 19744441075
the lipopolysaccharides (o-antigens) of rhodopseudomonas viridis. 19744441220
electrochemical proton gradient and phosphate potential in bacterial chromatophores. 19744442600
[effect of ammonium on hydrogen evolution and nitrogen fixation in rhodopseudomonas palustris]. 19744453204
proceedings: electron transport control by energy-rich phosphates in rhodopseudomonas palustris. 19744461531
genetic recombination in rhodopseudomonas capsulata.a system for genetic exchange in rhodopseudomonas capsulata has been discovered. each genetic marker thus far examined can be transferred, and many strains of rps. capsulata can participate in genetic exchange. the mechanism of gene transfer seems unlike that of any previously described bacterial system, since genes can be transferred by cell-free filtrates, but the vector is resistant to deoxyribonuclease and has a sedimentation constant of about 70 s.19744522805
sulfite reductase activity in extracts of various photosynthetic bacteria.extracts of representative bacterial strains from the various families of photosynthetic prokaryotes are demonstrated to possess significant levels of sulfite reductase [ec 1.8.99.1; hydrogen-sulfide: (acceptor)oxidoreductase] activity with reduced methyl viologen as electron donor, but not nadph(2). the enzyme is localized primarily in the soluble fraction of the extracts, in contrast to adenylysulfate reductase [ec 1.8.99.2; amp, sulfite: (acceptor) oxidoreductase], which is bound normally in ...19744526215
on reconstitution of bacterial photophosphorylation in vitro.membranes active in oxidative phosphorylation but inactive in photophosphorylation were isolated from a carotenoid-less and bacteriochlorophyll-less mutant strain of rhodopseudomonas capsulata. these membranes were uncoupled and recombined with coupling factor from the same strain and with reaction centers from a photosynthetically active revertant strain. the reconstituted system was able to form atp from adp and inorganic phosphate under anaerobic conditions in the light. rates of 2 nmoles of ...19744530296
resonance-raman evidence for anomalous heme structures in cytochrome c' from rhodopseudomonas palustris. 19744366150
properties of the cytochrome a-like material developed in the photosynthetic bacterium rhodopseudomonas spheroides when grown aerobically. 19744367961
preliminary crystallographic data for cytochrome c' of rhodopseudomonas palustris. 19744369031
6-o-methyl-d-glucosamine in lipopolysaccharides of rhodopseudomonas palustris strains. 19744369456
a thermodynamic characterisation of the cytochromes of chromatophores from rhodopseudomonas capsulata. 19744369739
in situ characterisation of photosynthetic electron transport in rhodopseudomonas capsulata. 19744370093
on the question of the primary acceptor in bacterial photosynthesis:manganese substituting for iron in reaction centers of rhodopseudomonas spheroides r-26. 19744371037
adaptation in rhodopseudomonas spheroides. 19744371623
energy transduction in photosynthetic bacteria. the nature of cytochrome c oxidase in the respiratory chain of rhodopseudomonas capsulata. 19744372102
the photo-oxidation of horse heart cytochrome c and native cytochrome c2 by reaction centres from rhodopseudomonas spheroides r26. 19744373062
flash photolysis-electron spin resonance study of the effect of o-phenanthroline and temperature on the decay time of the esr signal b1 in reaction-center preparations and chromatophores of mutant and wild strains of rhodopseudomonas spheroides and rhodospirillum rubrum. 19744373063
influence of iron deficiency on the cytochromes of rhodopseudomonas spheroides. 19744373246
regulation of biosynthesis of aspartate family amino acids in the photosynthetic bacterium rhodopseudomonas palustris. 19744374151
[initial pathways of pyruvate metabolism in phototrophic bacteria]. 19744374642
different pathways for fructose and glucose utilization in rhodopseudomonas capsulata and demonstration of 1-phosphofructokinase in phototrophic bacteria. 19744277436
histone analysis in volvox. 19744277508
[inhibition of porphyrin biosynthesis in rhodopseudomonas spheroides under the effect of exogenous glucose and atp without modifications of the ala-synthetase and ala-dehydratase activities]. 19744279739
[effect of an inhibitor isolated from rhodopseudomonas spheroides on porphyrin biosynthesis by rat liver homogenate]. 19744282285
[growth in the dark and the nadh-oxidase activity of rhodopseudomonas palustris]. 19744151335
reconstitution of light-dependent electron transport in membranes from a bacteriochlorophyll-less mutant of rhodopseudomonas spheroides. 19744153913
[lipopolysaccharides from rhodospirillaceae: composition and taxonomic relevance]. 19744154665
[nitrate utilization by purple bacteria]. 19744155788
characterization of reaction centers from photosynthetic bacteria. ii. amino acid composition of the reaction center protein and its subunits in rhodopseudomonas spheroides r-26. 19744206664
bacteriocinogeny in the athiorhodaceae. 19744209388
modulation by fumarate of a pi-insensitive pyruvate kinase from rhodopseudomonas capsulata. 19744216333
[ribulose diphosphate and phosphopyruvate carboxylase activity in phototropic bacteria]. 19744217882
characterization of reaction centers from photosynthetic bacteria. i. subunit structure of the protein mediating the primary photochemistry in rhodopseudomonas spheroides r-26. 19744132124
amino acid sequence at the haem region of cytochrome c2 from rhodopseudomonas palustris, strain x. 19744136519
citrate lyase from rhodopseudomonas gelatinosa: purification, electron microscopy and subunit structure. 19744141604
bacteriophages of rhodopseudomonas spheroides: isolation and characterization of a rhodopseudomonas spheroides bacteriophage.a dna-containing bacteriophage, designated rs1, infecting rhodopseudomonas spheroides 2.4.1, has been isolated from sewage. the buoyant density of rs1 in cscl equilibrium centrifugation is 1.50 g/cm(3), and the buoyant density of rs1 dna is 1.706. the phage possesses a polyhedral head, approximately 65 nm in diameter, and a tail 60 nm long. when grown on aerobic cells, rs1 has a latent period of 120 min and an average burst size of 20. when grown on anaerobic cells, rs1 has a latent period of 15 ...19744545531
h+ uptake by chromatophores from rhodopseudomonas spheroides. the relation between rapid h+ uptake and the h+ pump. 19744546206
a comparison of beauvericin, enniatin and valinomycin as calcium transporting agents in liposomes and chromatophores. 19744546705
comparative studies on two reaction center preparations from rhodopseudomonas speheroides y. 19744546760
effects of extraction and replacement of ubiquinone upon the photochemical activity of reaction centers and chromatophores from rhodopseudomonas spheriodes. 19744547199
function of membrane proteins coupled to bacteriochlorophyll synthesis. studies with wild type and mutant strains of rhodopseudomonas spheroides. 19744547213
kinetics of photosynthetic membrane protein assembly in rhodopseudomonas spheroides. 19744547239
the state of chlorophyll and carotenoid in vivo. ii. a linear dichroism study of pigment orientation in photosynthetic bacteria. 19744547333
primary events in photosynthesis: picosecond kinetics of carotenoid bandshifts in rhodopseudomonas spheroides chromatophores. 19744547716
isolation and characterization of a temperate bacteriophage specific for rhodopseudomonas spheroides.the isolation of a temperature phage specific for the photosynthetic bacterium rhodopseudomonas spheroides is reported. this phage, rphi-1, establishes a state of lysogeny and can be induced from the prophage state by exposure to mitomycin c or uv irradiation. mutants of rphi-1 which grow on a standard laboratory strain (2.4.1) of rhodopseudomonas spheroides were isolated. although the original rphi-1 isolated was chloroform sensitive, the mutant which plates on strain 2.4.1 is chloroform resist ...19744547800
control of delta-aminolevulinate synthetase activity in rhodopseudomonas spheroides. iii. partial purification of the fraction i activating enzyme and the occurrence of two forms of fraction ii. 19744548063
small angle x-ray scattering of the thylakoid membranes of rhodopseudomonas speroides in aqueous suspensions.the diffraction patterns of particles which have the shape of hollow spheres, i.e. vesicles, can be satisfactorily analyzed by means of a new formula of weick (1974). this formula is used for the small angle x-ray scattering analysis of aqueous suspensions of thylakoids of rhodopseudomonas spheroides. some essential results are: (a) the membrane has a rather asymmetric structure with one layer of low electron density at its inner side and two layers of high electron density near the outer surfac ...19744545071
topology and growth of the intracytoplasmic membrane system of rhodopseudomonas spheroides: protein, chlorophyll, and phospholipid insertion into steady-state anaerobic cells.an equilibrium density gradient centrifugation study involving the separation of "old" and "new" membranes has been developed to determine the manner in which protein, lipid, and chlorophyll are incorporated into growing intracytoplasmic membranes (chromatophores) of rhodopseudomonas spheroides. chromatophores derived from cells grown in an h(2)o-medium had a density of 1.175 to 1.180 g/cm(3) and were readily separable from chromatophores having a density of 1.220 to 1.230 isolated from cells gr ...19744545399
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