Publications

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[angiotomography of supratentorial brain tumors]. 2003207124
effects of potassium azide on soil microbial populations and soil enzymatic activities.preplant applications of potassium azide (kn3) to pine nursery beds were evaluated for effect on the soil microflora and on soil enzyme activity where either plastic-sealing or water-sealing techniques were used. two weeks after incorporation of azide (0-224 kg/hs), soil samplings revealed reduced populations of bacteria and fungi and a corresponding decline in invertase and amylase activities. these effects were proportionate to the amount of azide used and were more pronounced in plastic-seale ...2003164268
acute toxicity studies of some new organic trivalent antimonials. 2003262475
[ewing's sarcoma in a 13-month-old infant]. 2003153292
[hemodilution therapy in peripheral arterial occlusive disease]. 20041618610
c-heterochromatin variation and transposition in tumor progression. 20043066156
[the control of renin secretion: experimental data and clinical implications]. 2004356832
colonic adrenocarcinomas in patients with ureterosigmoidostomies.two additional cases of adenocarcinoma of the sigmoid colon occurring at the site of ureterosigmoidostomy are reported. the high risk of this complication occurring in patients who have undergone ureterosigmoidostomy is described. the possible shortness of the interval from diversion to development of an adenocarcinoma, especially in those cases involving carcinoma of the bladder, is emphasized. it is strongly recommended that careful follow up of such patients should begin within several years ...2004446246
vitamin a deprivation and drosophila photopigments. 2004404571
tomographic localization of motion axes.localization of motion axes within joints and junctures in living humans has been carried out by means of roentgen stereophotogrammetry. another technique is presented here, in which the localization of the axis is carried out by means of tomography. the field of application for this technique has been examined in a series of phantom experiments. it may be employed in certain situations where roentgen stereophotogrammetry is not useful. no other equipment is needed than a pluridirectional tomogr ...2004525421
a case of missing justification in an audit of thyroidectomy. 2004404608
characterization of the anaplasma marginale msp2 locus and its synteny with the omp1/p30 loci of ehrlichia chaffeensis and e. canis.major surface protein 2 (msp2) is an immunodominant and antigenically variant protein in the outer membrane of the rickettsia anaplasma marginale. msp2 variation is generated by recombination into a single operon-linked genomic expression site. the complete 5.6-kb msp2 locus was identified by sequencing a 90-kb region of the st. maries strain of a. marginale. the locus encoded, in a 5' to 3' direction, a transcriptional regulator followed by five outer membrane proteins, omp1, opag3, opag2, opag ...200414697516
antinuclear antibodies can be detected in dog sera reactive to bartonella vinsonii subsp. berkhoffii, ehrlichia canis, or leishmania infantum antigens.the presence of antinuclear antibodies (anas) is used to support a clinical diagnosis of systemic lupus erythematosus (sle) in dogs. however, clinicians must interpret the detection of anas with caution, particularly in light of increasing evidence that dogs with known bacterial and protozoal infections can have high ana titers. retrospectively, medical records were reviewed for all dogs that were concurrently tested for antinuclear antigens and bartonella vinsonii (berkhoffii), ehrlichia canis, ...200414765731
a serosurvey of hepatozoon canis and ehrlichia canis antibodies in wild red foxes (vulpes vulpes) from israel.a seroepidemiological survey was conducted to investigate the prevalence of antibodies reactive with ehrlichia canis and hepatozoon canis antigens in free-ranging red foxes (vulpes vulpes) in israel. of 84 fox sera assayed, 36% were seropositive for e. canis by the indirect fluorescent antibody (ifa) test and 24% were positive for h. canis using an enzyme-linked immunosrbent assay (elisa). canine ehrlichiosis and hepatozoonosis appear to be endemic in the wild red fox populations in israel, and ...200415036573
experimental acute canine monocytic ehrlichiosis: clinicopathological and immunopathological findings.clinical signs, humoral and cellular immune responses, and microscopic and gross tissue alterations resulting from acute experimental ehrlichia canis infection in dogs were studied. four dogs were inoculated with e. canis and four were used as uninfected controls. after a 10-14-day incubation period, infected dogs developed pyrexia up to 41 degrees c for 6-8 days. antibody titers to e. canis antigen were demonstrable in all inoculated dogs at 30 days post-infection. necropsy of infected animals ...200415036578
the relationship between the degree of thrombocytopenia and infection with ehrlichia canis in an endemic area.ehrlichia canis is the causative agent of canine monocytic ehrlichiosis. in order to evaluate platelet counts as a screening test for e. canis in an endemic area, 217 whole blood samples from dogs were divided into three groups: 71 non-thrombocytopenic samples (group a, platelet counts greater than 200 000/ml) and 146 thrombocytopenic samples (less than 200 000/ml). the thrombocytopenic group was further divided into 62 with platelet counts between 100 000-200 000/ml (group b) and 84 samples wit ...200415099511
chronic canine ehrlichiosis (ehrlichia canis): a retrospective study of 19 natural cases.nineteen dogs from greece with chronic ehrlichiosis were studied. the dogs exhibited bicytopenia or pancytopenia, bone marrow hypoplasia, seroreactivity to ehrlichia canis (e. canis) antigens, and had no history of drug or radiation exposure. anorexia, depression, severe bleeding tendencies, hypoalbuminemia, and increased serum alanine aminotransferase activity were also hallmarks of the disease. all these animals eventually died, irrespective of the treatment applied. some dogs were also serolo ...200415131097
infection and blood transfusion: a guide to donor screening.in recent years, blood-component therapy has become more accessible in veterinary practice. as with human medicine, care must be taken to minimize the risk of disease transmission from donor to recipient. determining the appropriate diseases to screen for is complicated by regional variations in disease incidence, the existence of chronic carrier states for some diseases, the difficulty in screening-test selection, and testing cost. the feline diseases considered include retroviral infections, f ...200415179926
[coxiella burnetii: what is the reality?].after the second world war, in italy q fever or coxiellosis has been shown a significant relevance, a recrudescence with an epidemic state for over ten years. later, the infectious disease occurred as endemic since the 80s, the outbreaks were just isolated. workflows analysis of some authors has demonstrated the spread out of the infection throughout italian herds with a prevalence ranging from 1.2 per cent to 10 per cent. our survey carried out throughout campania area in cattle has shown a rea ...200415305702
seroepidemiological study of canine ehrlichial infections in yamaguchi prefecture and surrounding areas of japan.randomly selected serum samples from 150 dogs from yamaguchi and neighbouring prefectures were subjected to the indirect immunofluorescent assay to detect antibodies against ehrlichia canis, ehrlichia chaffeensis, ehrlichia muris and ehrlichia from ixodes ovatus. a total of 30 out of the 150 serum samples reacted with at least one of the antigens at a titer of 1:20 or more. considerable cross-reactivity was seen and most samples reacted with at least two different antigens. fifteen (10.0%) dogs ...200415350665
bartonella henselae igg antibodies are prevalent in dogs from southeastern usa.in contrast to the large body of literature regarding bartonella henselae in humans and cats, there is little information about b. henselae as an infectious agent in dogs. due to the paucity of information regarding the b. henselae serology in dogs, we performed a cross-sectional serosurvey using b. henselae antigen in order to compare the seroprevalence between sick and healthy dogs from the south-eastern usa. ninety-nine sera were collected from clinically healthy dogs. three hundred and one s ...200415369661
the pcs20 pcr assay for ehrlichia ruminantium does not cross-react with the novel deer ehrlichial agent found in white-tailed deer in the united states of america.white-tailed deer are susceptible to heartwater (ehrlichia [cowdria] ruminantium infection) and are likely to suffer high mortality if the disease spreads to the united states. it is vital, therefore, to validate a highly specific and sensitive detection method for e. ruminantium infection that can be reliably used in testing white-tailed deer, which are reservoirs of antigenically or genetically related agents such as ehrlichia chaffeensis, anaplasma (ehrlichia) phagocytophilum (hge agent) and ...200415373331
comparison of results for serologic testing and a polymerase chain reaction assay to determine the prevalence of stray dogs in eastern tennessee seropositive to ehrlichia canis.to determine the prevalence of stray dogs in eastern tennessee seropositive to ehrlichia canis and examine the correlation between results for an elisa, indirect immunofluorescent antibody (ifa) test, and polymerase chain reaction (pcr) assay.200415478765
first isolation and molecular characterization of ehrlichia canis in spain.this paper reports the first isolation and culture of ehrlichia canis in spain from a naturally infected dog using the dh82 cell line. after dna extraction and pcr amplification, a nearly complete (1412bp) sequence of the 16s rrna gene of the new e. canis strain was obtained. the genbank accession number for the nucleotide sequence of this strain is ay394465. this sequence was aligned with the 16s rrna gene sequences of other ehrlichia strains accessible in genbank. the 16s rrna gene sequence of ...200415482892
comparison of simultaneous splenic sample pcr with blood sample pcr for diagnosis and treatment of experimental ehrlichia canis infection.this report presents evidence that dogs recover from acute canine monocytic ehrlichiosis (cme) after 16 days of doxycycline treatment (10 mg/kg of body weight every 24 h). blood pcr was as valuable as splenic aspirate pcr for early diagnosis of acute cme. splenic aspirate pcr was, however, superior to blood pcr for the evaluation of ehrlichial elimination.200415504892
free light-chain proteinuria and normal renal histopathology and function in 11 dogs exposed to lleishmania infantum, ehrlichia canis, and bbabesia canis.the purpose of this retrospective study was to investigate the relationship among proteinuria consisting of immunoglobulin free light chains (flcs), renal histopathologic findings, and routine markers of renal function in 11 dogs exposed to leishmania infantum (n = 8), ehrlichia canis (n = 2), and babesia canis (n = 1). flc proteinuria was suspected based on identification of a 22- to 27-kda band by sodium dodecyl sulfate-agarose gel electrophoresis (sds-age) and later confirmed by immunofixatio ...200415515575
acquired amegakaryocytic thrombocytopenia--four cases and a literature review.acquired amegakaryocytic thrombocytopenla was diagnosed in four dogs. initial platelet counts in all four dogs were less than 50,000 x 10(9)/litre and initial bone marrow examinations revealed megakaryocytic hypoplasia with minimal changes in the erythroid and myeloid cell lines. two dogs had evidence of idiopathic immune-mediated disease and two dogs had evidence of associated infectious disease. one dog had a positive antibody titre to borrella burgdorferi, and one dog had positive titres to b ...200415515801
evaluation of antibiotic susceptibilities of ehrlichia canis, ehrlichia chaffeensis, and anaplasma phagocytophilum by real-time pcr.we determined mics of antibiotics against anaplasma phagocytophilum, ehrlichia chaffeensis, and ehrlichia canis by real-time quantitative pcr. the doubling times of the organisms were established: 19 h for e. chaffeensis, 26 h for a. phagocytophilum, and 28 h for e. canis. in comparison to the reference method for determining sensitivities, which uses diff-quick staining, our pcr assay was very sensitive and specific. we confirmed that doxycycline and rifampin are highly active against these bac ...200415561862
mixed ehrlichia canis, hepatozoon canis, and presumptive anaplasma phagocytophilum infection in a dog.a 5-month-old, female, mongrel dog was admitted to the clinic of companion animal medicine, aristotle university of thessaloniki, greece, with depression, anorexia, fever, peripheral lymphadenopathy, splenomegaly, oculonasal discharge, nonregenerative anemia, and mild thrombocytopenia. cytology of giemsa-stained buffy coat, bone marrow, and lymph node aspiration smears revealed numerous morulae in mononuclear leukocytes and in neutrophils, and hepatozoon canis gamonts in neutrophils. the dog was ...200415570564
assessment of feline ehrlichiosis in central spain using serology and a polymerase chain reaction technique.antibodies to ehrlichia spp. and inclusion bodies compatible with ehrlichia spp. in feline blood cells have been previously detected in spain. the aim of this study was to assess the presence of antibodies to e. canis, n. risticii, and a. phagocytophilum in 122 feline serum samples from madrid (central spain). in addition, ehrlichia genus-specific, one-tube, nested polymerase chain reaction (pcr) was performed from blood samples from these cats. of the cats, 10.6% were seropositive for e. canis, ...200415604476
[ehrlichia and babesia infections in dogs in the netherlands].a retrospective study was performed at the department of clinical sciences of companion animals at utrecht university amongst 75 dogs diagnosed with a babesia canis and/or an ehrlichia canis infection. the majority of the dogs had visited an endemic area (most often the mediterranean area or the dutch antilles), but two dogs became infected with babesia in the netherlands. babesia infections were associated with a stay in an endemic area and an incubation period that are both significantly short ...200415622893
antibodies reactive with bartonella henselae and ehrlichia canis in dogs from the communal lands of zimbabwe.the prevalences of antibodies against bartonella henselae and ehrlichia canis were determined in sera from 228 dogs in 5 communal lands of zimbabwe, areas where traditional subsistence agro-pastoralism is practised. the sera were collected from apparently healthy dogs during routine rabies vaccination programmes and tested with indirect fluorescent antibody assays using b. henselae (houston-i) and e. canis (oklahoma) as antigens. we found reactive antibodies (> or =1:80) against b. henselae in 1 ...200415628802
diffuse cutaneous candidiasis in a dog. diagnosis by pcr-rea.the authors describe a clinical case of cutaneous candidiasis in a dog with dermatological lesions, characterized by persistent alopecia, crusts, ulcers and scales. predisposing factors such as the use of corticosteroids, the concomitan presence of an autoimmune disease (pemphigus foliaceus) and an infection of ehrlichiosis caused by ehrlichia canis were observed. histopathological findings included signs of orthokeratotic hyperkeratosis, moderate follicular keratosis and light epidermic acantho ...200415709788
molecular and serologic evidence of tick-borne ehrlichiae in three species of lemurs from st. catherines island, georgia, usa.in recent years, several species of ehrlichiae have been recognized as tick-borne disease agents of veterinary and medical importance. clinically normal free-ranging or previously free-ranging lemurs, including 46 ring-tailed lemurs (lemur catta), six blue-eyed black lemurs (eulemur macaco flavifrons), and four black and white ruffed lemurs (varecia variegata variegata) from st. catherines island, georgia, were tested for evidence of exposure to tick-borne ehrlichiae. all 52 adult lemurs were se ...200415732591
anti-hepatozoon canis serum antibodies and gamonts in naturally-occurring canine monocytic ehrlichiosis.the prevalence of igg antibodies to hepatozoon canis and the presence of gamonts in the blood and hemolymphatic tissues were studied in dogs with canine monocytic ehrlichiosis (cme) caused by ehrlichia canis. both pathogens are transmitted by the tick rhipicephalus sanguineus. forty-five out of 69 (65.2%) dogs with cme were seropositive to h. canis by an enzyme-linked immunosorbent assay (elisa). intra-neutrophilic gamonts of h. canis were found in 2 out of 69 dogs (2.9%) comprising 4.5% of the ...200515845277
validation of chemoprevention of canine monocytic ehrlichiosis with doxycycline.epidemiological (cohort follow-up) and laboratory techniques studies were done to validate a programme of chemoprevention of canine monocytic ehrlichiosis (cme) with this molecule. 614 dogs returning to france after having spent at least 4 months in a cme-endemic area (africa, guyana, middle-east, etc.) were the object of systematic serological testing by indirect immunofluorescence (ifa). the dogs were given 100 mg of doxycycline per os daily for chemoprevention of cme. in addition, hplc (high ...200515863288
transstadial and intrastadial experimental transmission of ehrlichia canis by male rhipicephalus sanguineus.the acquisition and transmission of rickettsial pathogens by different tick developmental stages has important epidemiological implications. the purpose of this study was to determine if male rhipicephalus sanguineus can experimentally acquire and transmit ehrlichia canis in the absence of female ticks. two trials were performed where nymphal and male r. sanguineus were simultaneously acquisition fed on the same infected donor hosts, and transstadially or intrastadially exposed male ticks were f ...200515941624
molecular detection and characterization of ehrlichia canis from dogs in turkey.seroprevalence of ehrlichia canis antibodies among dogs in turkey were previously reported, however, the ehrlichial organism has never been characterized in this region. the current study examined dogs from ankara with febrile illness for e. canis infection with e. canis-specific pcr. three of the 12 blood specimens from dogs showing clinical signs compatible with canine ehrlichiosis were found to be positive by pcr using e. canis-specific primers. e. canis detected in one of the blood specimens ...200516048040
serological evidence of ehrlichia spp. exposure in cats from northeastern spain.there is little information about ehrlichia canis as an infectious agent in cats. in order to estimate the prevalence of antibodies to e. canis in the feline population, 235 cat sera were analysed by indirect fluorescent-antibody test. with the objective to determine some risk factors associated with seropositivity, serum samples were divided into two groups: urban stray cats and pet cats. the seroprevalence detected was 17.9%. most positive sera (83.3%) showed low antibody titres (<1:80). serop ...200516115100
evaluation of neutrophil oxidative metabolism in canine monocytic ehrlichiosis.canine monocytic ehrlichiosis (cme) is a tick-borne disease caused by ehrlichia canis, a rickettsia that infects the monocytes of dogs. this infection can result in a chronic and life-threatening disease. thrombocytopenia, mild anemia, and leukopenia are the most common hematologic findings in cme.200516134067
a preliminary study to evaluate the immune responses induced by immunization of dogs with inactivated ehrlichia canis organisms.ehrlichia canis is an intracellular pathogen that causes canine monocytic ehrlichiosis. although the role of antibody responses cannot be discounted, control of this intracellular pathogen is expected to be by cell mediated immune responses. the immune responses in dogs immunized with inactivated e. canis organisms in combination with quil a were evaluated. immunization provoked strong humoral and cellular immune responses, which were demonstrable by western blotting and lymphocyte proliferation ...200516137129
prevalence of ehrlichia canis infection in thrombocytopenic dogs from rio de janeiro, brazil.infection with ehrlichia canis causes a highly variable, multisystemic disease in dogs. nevertheless, many clinicians in rio de janeiro, brazil, use the presence of only thrombocytopenia to make a presumptive diagnosis of e canis infection.200515732017
ocular signs of canine monocytic ehrlichiosis: a retrospective study in dogs from barcelona, spain.canine monocytic ehrlichiosis (cme) is a tick-borne disease caused by the rickettsia ehrlichia canis. ocular lesions are a common feature of the disease and can be present in all stages. the purpose of this retrospective study was to determine the prevalence, type and response to treatment of ocular lesions associated with monocytic ehrlichiosis in 46 affected dogs presented to the autonomous university of barcelona-veterinary teaching hospital (uab-vth) from january 2000 to december 2002. dogs ...200516359361
ehrlichial infection in cameroonian canines by ehrlichia canis and ehrlichia ewingii.ehrlichia chaffeensis and ehrlichia ewingii are agents of emerging human ehrlichioses in north america and are transmitted primarily by amblyomma americanum ticks, while ehrlichia canis is the globally distributed cause of canine monocytic ehrlichiosis (cme) and is transmitted by the brown dog tick, rhipicephalus sanguineus. although e. canis and ehrlichia ruminantium are endemic in africa, the presence of ehrlichial agents in dogs and ticks in cameroon has not been investigated. the objective o ...200516181750
suppurative, nonseptic polyarthropathy in dogs.the goals of this study were to determine the historical, physical examination, and clinicopathologic findings in dogs with suppurative, nonseptic polyarthropathy and to identify concurrent disorders associated with this syndrome. medical records of 52 dogs with cytologic evidence of suppurative inflammation in two or more joints were examined retrospectively. age of dogs was 4.8 years (median, range: 0.5-12 years). there was no clear breed or sex predilection, but most were large-breed dogs (bo ...200516231709
detection of medically important ehrlichia by quantitative multicolor taqman real-time polymerase chain reaction of the dsb gene.ehrlichia species are the etiological agents of emerging and life-threatening tick-borne human zoonoses, in addition to causing serious and fatal infections in companion animals and livestock. we developed the first tricolor taqman real-time polymerase chain reaction assay capable of simultaneously detecting and discriminating medically important ehrlichiae in a single reaction. analytical sensitivity of 50 copies per reaction was attained with templates from ehrlichia chaffeensis, ehrlichia ewi ...200516237220
feline leishmaniasis and ehrlichiosis: serological investigation in abruzzo region. 200516244984
genetic variants of anaplasma phagocytophilum infecting dogs in western washington state.eight dogs from western washington state suspected of being infected with anaplasma phagocytophilum because of the finding of morulae in peripheral blood neutrophils were studied for determination of the etiologic agent of disease. all cases were diagnosed between april 2003 and april 2004. six of the eight dogs had no travel history during the 6 months prior to presentation. two dogs had traveled within the northwest united states and canada. fever, lethargy, and anorexia were the most common c ...200515695682
cytokine responses in dogs infected with ehrlichia canis oklahoma strain.ehrlichia canis causes persistent infection of monocytes/macrophages and causes canine monocytic ehrlichiosis. other than antibody development, very little is known how the dog immune system responds to this pathogen. in the present study, we examined the temporal expression of cytokine mrna in peripheral blood mononuclear cells (pbmcs) from dogs inoculated with e. canis oklahoma strain. we also examined production of interferon-gamma (ifn-gamma) by canine pbmcs infected with e. canis in vitro.200516481554
molecular characterization of e. canis gp36 and e. chaffeensis gp47 tandem repeats among isolates from different geographic locations. 200516481555
an immunoreactive 38-kilodalton protein of ehrlichia canis shares structural homology and iron-binding capacity with the ferric ion-binding protein family.ehrlichiae are tick-transmitted, gram-negative, obligately intracellular bacteria that live and replicate in cytoplasmic vacuoles, but little is known about iron acquisition mechanisms necessary for their survival. in this study, a genus-conserved immunoreactive ferric ion-binding protein (fbp) of ehrlichia canis was identified and its iron-binding capability was investigated. e. canis fbp was homologous to a family of periplasmic fbp's involved in iron acquisition and transport in gram-negative ...200515618141
ehrlichia canis in dogs in yucatan, mexico: seroprevalence, prevalence of infection and associated factors.the aim of this study was to determine the prevalence of infection, seroprevalence, and factors associated with antibody response to ehrlichia canis in dogs of yucatan, mexico. the study was carried out in four veterinary clinics located in merida, the capital city of yucatan, mexico. blood samples were obtained from a total of 120 dogs, and each animal was physically examined to determine age and sex, as well as to record any clinical signs of platelet-related bleeding. blood samples were analy ...200515619376
[cutaneous evaporimetry. iii. cutaneous water loss (cwl) in the suckling infant]. 2005548079
clinical evaluation of tumor scanning with 169-yb-citrate.169-yb-citrate was used as the tumor-scanning agent in 360 patients with malignant tumors and in 55 others with benign lesions and the results compared with 67-ga-citrate studies in other series. in malignant tumors, the overall positive detection rate for ytterbium was 65.3%; in benign lesions, the false-positive rate was 29.1%. good results were obtained in the extremities, head and neck, and pelvic area. of all tumors visualized, squamous cell carcinoma was detected most effectively (77.5%). ...20051153741
ripple suppression during reconstruction in transverse tomography. 20051187793
designing for dentistry. 20053113903
payments to policyholders and beneficiaries exceed four billion dollars. 20051273867
inhibition of drug metabolism by quinolone antibiotics.a number of quinolone antibiotics have been found to reduce the hepatic clearance of coadministered drugs such as theophylline. enoxacin appears to be the most potent inhibitor, consistently decreasing theophylline clearance by more than 50%, while a single study suggests a similar degree of inhibition with pipemidic acid. ciprofloxacin and pefloxacin reduce theophylline clearance to a smaller extent (approximately 20 to 30%). however, with ciprofloxacin, larger changes and theophylline toxicity ...20053052987
[post-cholecystectomy condition: duodeno-gastric reflux and bile acid concentration in the gastric juice].in cholecystectomized patients highly significantly more frequently a duodenogastric reflux was found than in a group of patients with a healthy abdomen and a group of patients with cholelithiasis. the average concentration of bile acid in the gastric juice was after the removal of the gall-bladder manifoldly higher than in the control groups. the number of patients with concentrated reflux was also highly significantly larger than in patients with cholelithiasis not operated on and in patients ...2005538988
phobic response factors from the fear survey schedule.a phobic response scale was factored from responses to the fear survey schedule. subjects were 137 male and 168 female college students. a six factor solution extracted 41.78% of the trace. the factors were as follows: i (hostile-dependence), ii(body assult), iii (developmental fears), iv (performance and evaluation), v (death evasion), and vi (nuisance animals). an analysis of sex differences by item and scale generally supported and extended previous research. some conceptual ties were made by ...20051151900
duplication of radiographs. 20051074385
[resection of chondrosarcoma of the sternum with restoration of the thoracic wall. report of a case]. 20051081246
[vascular surgery in the aged]. 2005538948
[gastric secretion in gastroduodenal ulcer]. 2005545511
what is a biological? 20051888493
[abo blood groups in gastric cancer]. 20051169525
psychiatric morbidity in a sample of a london coroner's open verdicts.one hundred and thirty-four deaths recorded as open verdicts in the inner west london coroner's district during 1969 and 1970 have been surveyed for evidence of mental illness. for 110 (82 per cent) of these deaths the probable verdicts were suicide or accident and they were reclassified as undetermined deaths. of these deaths 73 per cent were diagnosed as mentally ill, 54 per cent were receiving medical treatment for psychological symptoms before death, 42 per cent had a history of psychiatric ...20051182365
interleukin-1 inhibits pge2 binding to macrophage-like p388d1 cells by a cyclic amp-independent process.the interaction between interleukin il-1 alpha and pge2 on p388d1 cells has been investigated. preincubation of murine macrophage-like cells, p388d1, with il-1 alpha (0-73 pm) reduced the binding of pge2 to these cells in a concentration-dependent manner. scatchard analysis showed that il-1 alpha decreased the pge2 binding by lowering both the high and low affinity receptor binding capacities (from 0.31 +/- 0.02 to 0.12 +/- 0.01 fmol/10(6) cells for the high affinity receptor binding sites and f ...20051310627
"raynaud's disease" in migraineurs: one entity or two?two cases are reported of patients who were diagnosed as having both migraine and raynaud's disease. because they were able to achieve effective control of both headaches and extremity temperature after undergoing a comprehensive approach--utilizing medication, biofeedback, and psychotherapy--it is suggested that both conditions are manifestations of the same disorder. it is recommended that a first step in approaching concurrent diagnoses of this type involve combined treatment as is often used ...20061446993
oesophageal intramural pseudodiverticulosis with similar lesions in the duodenum.oesophageal intramural pseudodiverticulosis is characterized by a segmental or diffuse distribution of numerous small outpouchings varying in size from 1 to 5 mm. review of previously described cases revealed a significant incidence of associated debilitating systemic disorders such as diabetes mellitus, malignancy, alcoholism and tuberculosis. no record could be found in the literature of a similar process occurring in other parts of the gastro-intestinal tract. a case is reported of a severe a ...20061797795
a common precursor for cd4+ t cells producing il-2 or il-4.to investigate whether cd4+ t cells are predetermined to produce a given pattern of lymphokines, we have used a culture system that allows the controlled induction of either il-2- or il-4-producing cd4+ t cells. single, freshly isolated murine cd4+ t cells were activated with con a, ril-2, and apc; the developing clones were split and then cultured for an additional 14 days with either ril-2 alone or with ril-2 and anti-cd3 stimulation. subclones expanded in the presence of ril-2 alone produced ...20061346619
supporting collaboration through a nursing informatics curriculum stage ii.collaboration is at the center of the process used to design, implement and evaluate an integrated informatics curriculum in a baccalaureate nursing program. this paper describes the second stage of a process to design the informatics nursing courses. the challenges to foster faculty collaborative relationships as well as to enhance the course content of all nursing informatics curriculum. a number of strategies were used to develop the collaborative efforts between the faculty and nursing staff ...20061482910
an enhanced-response system for performing chemiluminescent and bioluminescent tests. 20061366493
site of injection for vaccination. 20061392898
[familial hypercholesterolemia]. 20063291343
myocardial entrapment by lymphoma: a cause of reversible segmental left ventricular dysfunction. 20061636600
[why do physicians and patients ever more often resort to practitioners of alternative medicine: the healer and the quack?]. 20061563606
rediscovering wormwood: qinghaosu for malaria. 20061347342
primary fibrinogenolysis complicating second trimester placental separation.we present a case of second trimester placental separation complicated by severe bleeding diathesis. primary fibrinogenolysis is suggested as the cause of the coagulopathy.20061550391
[digestive hemorrhage caused by aorto-duodenal fistula. report of 5 cases].the aortoduodeonal fistula is a rare condition and has high mortality. we report five retrospective cases of digestive hemorrhage due to an aortoduodenal fistula (4 secondary and 1 primary) between 1986 and 1990. three of them died. attention is called to the importance of clinical suspicion and the main diagnostic methods in early diagnosis such as oral endoscopy. we also enhance the importance of emergency surgery for survival.20061599763
platelet aggregation studies in acute experimental canine ehrlichiosis.thrombocytopenia is the most common and consistent hematologic finding in patients with canine monocytic ehrlichiosis. dogs that recover from the severe thrombocytopenia still show bleeding tendencies, which suggest that platelet dysfunction is present.200616511794
comparison of an indirect immunofluorescence assay, western blot analysis, and a commercially available elisa for detection of ehrlichia canis antibodies in canine sera.to examine the correlation between results for an indirect immunofluorescence assay (ifa) that uses ehrlichia canis antigen as a substrate (ie, e canis-ifa), 2 western blot (wb) analyses, and a commercially available elisa in the detection of e canis antibody in dog sera.200616454622
a serological study of exposure to arthropod-borne pathogens in dogs from northeastern spain.there is limited information regarding the prevalence of many vector borne pathogens in europe and especially in spanish dogs. we investigated 206 sick and 260 clinically healthy dogs from three different regions in northeastern spain for antibodies to rickettsia conorii (rc), ehrlichia canis (ec), anaplasma phagocytophilum (ap), bartonella henselae (bh), bartonella vinsonii subsp. berkhoffii (bvb), leishmania infantum (li) and borrelia burgdorferi (bb) and for antigen of dirofilaria immitis (di ...200616472522
bone marrow mastocytosis in dogs with myelosuppressive monocytic ehrlichiosis (ehrlichia canis): a retrospective study.bone marrow mastocytosis has been reported rarely in naturally occurring canine monocytic ehrlichiosis (cme).200616967415
rickettsial agents in egyptian ticks collected from domestic animals.to assess the presence of rickettsial pathogens in ticks from egypt, we collected ticks from domestic and peridomestic animals between june 2002 and july 2003. dna extracts from 1019 ticks were tested, using pcr and sequencing, for anaplasma spp., bartonella spp., coxiella burnetii, ehrlichia spp., and rickettsia spp. ticks included: 29 argas persicus, 10 hyalomma anatolicum anatolicum, 55 hyalomma anatolicum excavatum, 174 hyalomma dromedarii, 2 hyalomma impeltatum, 3 hyalomma marginatum rufipe ...200617004028
longitudinal analysis of tick densities and borrelia, anaplasma, and ehrlichia infections of ixodes ricinus ticks in different habitat areas in the netherlands.from 2000 to 2004, ticks were collected by dragging a blanket in four habitat areas in the netherlands: dunes, heather, forest, and a city park. tick densities were calculated, and infection with borrelia burgdorferi and anaplasma and ehrlichia species was investigated by reverse line blot analysis. the lowest tick density was observed in the heather area (1 to 8/100 m2). in the oak forest and city park, the tick densities ranged from 26 to 45/100 m2. the highest tick density was found in the du ...200617028227
fatal systemic phaeohyphomycosis caused by ochroconis gallopavum in a dog (canis familaris).a 5-year-old shetland sheepdog was presented with a history of weakness, ataxia, anemia, thrombocytopenia, and occasional seizures. the dog had been treated for 6 months with prednisone for inflammatory bowel disease. a positive titer for ehrlichia canis was detected 6 months before referral. the initial physical examination revealed a weak, laterally recumbent dog with pale mucous membranes. neurologic examination revealed multiple neurologic deficits. a complete blood cell count (cbc) revealed ...200617099156
platelet aggregation and haemostatic response in dogs naturally co-infected by leishmania infantum and ehrlichia canis.haemostatic alterations in dogs naturally infected by ehrlichiosis and/or leishmaniasis were studied. platelet count, adp and collagen-induced platelet aggregation, prothrombin time, activated partial thromboplastin time (aptt) and plasma fibrinogen concentration were measured. an evident reduction of platelet aggregation response was shown for leishmania-ehrlichia co-infected dogs where platelet aggregation was lower in comparison with control and leishmaniotic dogs (adp and collagen, p < or = ...200617105577
human infection with ehrlichia canis accompanied by clinical signs in venezuela.a total of 20 human patients with clinical signs compatible with human monocytic ehrlichiosis (hme), who were admitted to the emergency clinic in lara state, venezuela, were studied. thirty percent (6/20) patients were positive for ehrlichia canis 16s rrna on gene-specific polymerase chain reaction (pcr). compared with the u.s. strains, 16s rrna gene sequences from all six patients had the same base mutation as the sequence of the e. canis venezuelan human ehrlichia (vhe) strain previously isola ...200617114689
epidemiological survey of ehrlichia canis and related species infection in dogs in eastern sudan.the infection rates of ehrlichia canis and related species in dogs in eastern sudan were examined using molecular methods. among 78 dogs examined, 63 (80.8%), 19 (24.4%), and 26 (33.3%) were positive for e. canis, anaplasma platys, mycoplasma haemocanis, and "candidatus mycoplasma haemoparvum," respectively. among these, 30 dogs were single-positive: 25 for e. canis, 2 for a. platys, 1 for m. hemocanis, and 2 for "c. m. haemoparvum." the rest of the dogs (48.7%) were positive for two or more pat ...200617114754
surveys on seroprevalence of canine monocytic ehrlichiosis among dogs living in the ivory coast and gabon and evaluation of a quick commercial test kit dot-elisa.canine monocytic ehlichiosis (cme), an enzootic disease in africa, has been studied in canine blood samples (serum). these dogs, without any clinical sign of disease, were living in abidjan (ivory coast) and in several small villages located in northeasst gabon (ogooué ivindo). the results obtained by indirect fluorescent antibody (ifa) test, used as a point of reference, and by a quick test dot-elisa were compared. blood samples taken from 390 asymptomatic dogs in 2003 (137 in ivory coast and 2 ...200617114755
experimental infections in dogs with ehrlichia canis strain borgo 89.this study attemps to clarify the virulence and the pathogenicity of the borgo 89 strain of ehrlichia canis isolated from a sick dog in corsica (france). four unscathed beagles were intravenously injected with an inoculum of leukocytes infected with the borgo 89 strain and the animals were examined daily for clinical signs of disease, and blood samples were drawn at frequent intervals for biochemical and hematologic assessment. serologic (ifi) and pcr assays were also carried out. the results at ...200617114756
cytokine gene expression by peripheral blood leukocytes in dogs experimentally infected with a new virulent strain of ehrlichia canis.ehrlichia canis (e. canis) is a lipopolysaccharide (lps)-deficient obligatory intracellular bacterium that causes canine monocytic ehrlichiosis, a chronic febrile disease accompanied with hematological abnormality. this study analyzed temporal expression levels of il-1beta, il-2, il-6, il-8, ifn-gamma, and tnf-alpha mrna by peripheral blood leukocytes from dogs experimentally infected with a new virulent strain of e. canis by using real-time rt-pcr. relative levels of il-1beta and il-8 transcrip ...200617114759
molecular survey of ehrlichia canis and anaplasma phagocytophilum from blood of dogs in italy.we investigated the prevalence of ehrlichia canis (e. canis) (n = 601) and anaplasma phagocytophilum (a. phagocytophilum) (n = 460) infection by means of real-time pcr from blood of italian dogs. the prevalence of e. canis in northern, central, and southern italy was 2.9%, 8%, and 9.7%, respectively. the prevalence of a. phagocytophilum was 0%.200617114768
a comparative study on oxidative stress in dogs infected with ehrlichia canis with or without concurrent infection with babesia gibsoni. 200617139542
direct identification of ehrlichia canis by a novel polymerase chain reaction method and molecular analysis of the citrate synthase (glta) gene from various italian strains.fourteen blood samples collected from dogs that were seropositive for ehrlichia canis were examined for the presence of the citrate synthase gene using a highly specific and sensitive novel polymerase chain reaction assay. the assay detected e. canis dna in 3 dogs. the complete nucleotide sequence of the citrate synthase gene was determined in 2 of the test-positive samples, and represents the first sequence of the gene to be derived from italian isolates. the sequence data displayed high identi ...200616617707
detection of ehrlichia chaffeensis in brazilian marsh deer (blastocerus dichotomus).ehrlichia chaffeensis was detected for the first time in blood samples from brazilian marsh deers (blastocerus dichotomus) captured in the marshes of parana river in southeast brazil in 1998. seven edta-blood samples from deers were analyzed by pcr and nested pcr for presence of ehrlichia chaffeensis, ehrlichia ewingii, ehrlichia canis, neoriickettsia risticii, anaplasma phagocytophilum and anaplasma marginale. three samples showed positive reactions for e. chaffeensis and anaplasma marginale. n ...200616621285
seroprevalence of anaplasma phagocytophilum among healthy dogs and horses in israel.the presence of reacting antibodies to anaplasma phagocytophilum has previously been demonstrated in israel, both in humans and the golden jackal (canis aureus syriacus). this study was undertaken to determine the seroprevalence of a. phagocytophilum antibodies in two additional potential hosts, domestic dogs and horses in order to investigate the possibility of exposure to the organism in israel. of 195 dogs tested, 9% were seroreactive with a. phagocytophilum antigen and 30% were seroreactive ...200616626404
[evaluation of ectoparasites and hemoparasites in dogs kept in apartments and houses with yards in the city of juiz de fora, minas gerais, brazil].fleas and ticks transmit various pathogens while feeding on the blood of dogs. this study sought to verify the occurrence of ectoparasitism and hemoparasitism in dogs from two urban areas in the city of juiz de fora, minas gerais, brazil. between february and august 2003, 101 dogs were studied: 50 came from apartments in the downtown region and 51 from houses with grassy yards. the ectoparasites were collected and conserved in etanol 70%. the occurrence of hemoparasites was verified by examining ...200616646996
seroepidemiology of canine babesiosis and ehrlichiosis in a hospital population.canine ehrlichiosis and babesiosis have a worldwide distribution with geographic variation in prevalence and main clinical manifestations. we prospectively determined seroprevalence of canine babesiosis and ehrlichiosis, and risk factors for seropositivity. three hundred and eighty-one dogs were randomly selected to represent the canine population at a veterinary teaching hospital in south brazil (latitude 23 degrees s). dogs were tested with a point-of-care elisa for ehrlichia canis antibodies ...200616647817
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