Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| nucleotide sequence of porcine insulin-like growth factor. 1:5' untranslated region, exons 1 and 2 and mrna. | 1990 | 2326169 | |
| cinnamon oil abuse by adolescents. | reports in the literature about cinnamon oil toxicity are limited to allergic reactions and local irritant effects from dermatologic exposure. cinnamon oil is easily obtained from pharmacies in 5-10 ml amounts for use as a flavoring agent and in craft items. within a 5-mo period the pittsburgh poison center (ppc) documented 32 cases of cinnamon oil abuse; all cases involved males aged 11-16 y and were reported to the ppc by school nurses. sucking on toothpicks or fingers which had been dipped in ... | 1990 | 2327068 |
| lipid soluble antioxidants preserve rabbit corneal cell function. | the survival time of rabbit corneal endothelial cells in isolated perfused cornea is extended dramatically by the addition of oxygen free radical scavengers. lipid soluble antioxidants were found to be more effective than water soluble non-enzymatic antioxidants in extending the survival time. the ultrastructural appearance of endothelial cells treated with vitamin e was compared with controls. vitamin e treated endothelial cells retained normal appearance up to 12h, but without vitamin e, break ... | 1990 | 2335108 |
| cloning and nucleotide sequence of a cdna encoding the antifungal-protein of aspergillus giganteus and preliminary characterization of the native gene. | 1990 | 2374718 | |
| diagnosis of acute typhus infection using the polymerase chain reaction. | we used the polymerase chain reaction (pcr) for the diagnosis of an acute rickettsial infection. a primer pair derived from the 17-kda antigen sequence of rickettsia rickettsii amplified specifically a 434-bp dna fragment from the genome of rocky mountain spotted fever and endemic and epidemic typhus. the assay was able to detect as few as 30 rickettsiae. detection of pcr-amplified dna with a digoxigenin-labeled dna probe confirmed an acute human infection with rickettsia prowazekii. | 1990 | 2378469 |
| endotoxin-induced uveitis in the rat: a hypothesis for preferential involvement of the anterior uvea. | endotoxin-induced uveitis (eiu) was produced in lewis rats by footpad injection of salmonella endotoxin. protein and cells were measured both in the aqueous humor and in the cerebrospinal fluid (csf) in order to examine if the inflammation was strictly limited to the anterior uvea. eiu was also induced in fischer and brown norway rats and the inflammation was compared among the three strains. although the function and structure of the choroid plexus is very similar to the ciliary body, no signs ... | 1990 | 2384004 |
| computer simulation of rocky mountain spotted fever transmission by the american dog tick (acari: ixodidae). | a computer model was developed for simulation of the transmission of rickettsia rickettsii, the causative agent of rocky mountain spotted fever (rmsf), by the american dog tick, dermacentor variabilis (say). the model of rmsf was combined with a model for population dynamics of the american dog tick and included simulation of infection and transmission of rickettsiae between ticks and host mammals and transmission of rmsf to humans. the model simulated the effects of biotic and environmental var ... | 1990 | 2388242 |
| "synaptic" ribbons in the pineal gland of the horse. | previous studies on the ultrastructure of the horse pineal gland did not report the presence of "synaptic" ribbons, functionally enigmatic pinealocyte organelles regularly occurring in other mammalian species. the aim of the present study was to reinvestigate the horse pinealocyte in this respect. careful investigations here reported allowed detection of "synaptic" ribbons in the pinealocytes of all the examined animals, although in a relatively low number (5.4 +/- 2.0 per 20,000 microns 2 of pi ... | 1990 | 2395075 |
| rat organum vasculosum laminae terminalis in vitro: responses to changes in sodium concentration. | extracellular action potentials were recorded from the organum vasculosum laminae terminalis (ovlt) in rat brain slice preparations; the effect of different concentrations of nacl on spontaneous firing frequency was studied. from 72 neurons, 67 (93%) were responsive to various perfusion media, while 5 neurons (7%) were not responsive. the change from the standard medium (sm; 124 mm nacl) to 99 mm, decreased the firing frequency in 24 (65%) and increased it in 13 (35%) out of 37 responsive cells. ... | 1990 | 2397412 |
| [tumors of the posterior fossa]. | 1990 | 258514 | |
| platelet aggregation in dogs experimentally infected with rickettsia rickettsii. | platelet aggregation studies were performed on nine beagle dogs experimentally infected with rickettsia rickettsia. platelets from dogs with rocky mountain spotted fever tended to be more aggregable than controls. | 1990 | 12684943 |
| [not immune from pseudoreligious rituals? parents fear youth sects - little can be done legally]. | 1990 | 258841 | |
| [studies on the improvement of radiotherapy of lung cancer (author's transl)]. | 1990 | 267900 | |
| a clinical preceptorship for new graduate nurses. | 1991 | 245388 | |
| medical microbiology. 4. | 1991 | 244946 | |
| national panel rejects radical mastectomy. | 1991 | 259007 | |
| [effect of anaprilin on the clinical course of stenocardia and the carbohydrate metabolic indices of ischemic heart disease patients in relation to the nature of their glucose tolerance]. | fourteen-day anaprilin treatment of coronary patients with varying glucose tolerance has demonstrated that the drug's therapeutic efficiency was greater in patients with normal glucose tolerance. anaprilin caused a moderate drop in baseline glucose level and considerably limited the degree of hyperglycemia in response to the glucose tolerance test in coronary patients with abnormally low glucose tolerance. insulin content was virtually unaffected by the drug, irrespective of the type of glucose ... | 1991 | 3327995 |
| oxidative inactivation of alpha 1-proteinase inhibitor by alveolar macrophages from healthy smokers requires the presence of myeloperoxidase. | the aim of this work was to study the ability of human alveolar macrophages (am) of 10 healthy smokers to inactivate alpha 1-proteinase inhibitor (alpha 1pi). purified alpha 1pi was incubated for 45 min, with human alveolar macrophages before and after stimulation by phorbol myristate acetate (pma) or opsonized zymosan. as a positive control, the same experiments were performed in parallel with blood human neutrophils (pmn). results are expressed as percentage of inactivation of alpha 1pi as eva ... | 1991 | 1657063 |
| efficacy of chloramphenicol, enrofloxacin, and tetracycline for treatment of experimental rocky mountain spotted fever in dogs. | dogs were experimentally inoculated with rickettsia rickettsii to characterize the comparative efficacies of chloramphenicol, enrofloxacin, and tetracycline for the treatment of rocky mountain spotted fever (rmsf). all three antibiotics were equally effective in abrogating the clinical, hematologic, and vascular indicators of rickettsial infection. antibiotic treatment for 24 h was sufficient to decrease the rickettsemia to levels below detection by vero cell culture. early treatment with all th ... | 1991 | 1666498 |
| genotypic identification of rickettsiae and estimation of intraspecies sequence divergence for portions of two rickettsial genes. | dna sequences from specific genes, amplified by the polymerase chain reaction technique, were used as substrata for nonisotopic restriction endonuclease fragment length polymorphism differentiation of rickettsial species and genotypes. the products amplified using a single pair of oligonucleotide primers (derived from a rickettsial citrate synthase gene sequence) and cleaved with restriction endonucleases were used to differentiate almost all recognized species of rickettsiae. a second set of pr ... | 1991 | 1671856 |
| dna polymorphism in the conserved 190 kda antigen gene repeat region among spotted fever group rickettsiae. | the 190 kda protein gene of r. rickettsii (r strain) contains a large region of tandemly arranged repeats. homologous sequences were discovered within the genomes of all but one species of spotted fever group rickettsiae. southern blots showed restriction fragment length polymorphisms between species of rickettsiae indicating structural differences among repeat regions. further analysis using polymerase chain reaction techniques indicated the repeat regions vary in size among rickettsiae. in add ... | 1991 | 1677592 |
| genetics of rickettsiae. | classical genetic approaches useful with free-living bacteria are difficult to apply to the rickettsiae. although rickettsial mutants have been isolated over the years, the genetic basis of these mutants is unknown, limiting their usefulness. the application of molecular biological techniques to rickettsial studies has provided the opportunity to isolate and study specific genes. genes encoding metabolic enzymes from rickettsiae were cloned in escherichia coli and shown to retain their regulator ... | 1991 | 1679397 |
| linkage of bipolar affective disorders to markers on chromosome 11p is excluded in a second lateral extension of amish pedigree 110. | linkage between markers on chromosome 11p and bipolar affective disorders can be excluded in a second large lateral extension of the original amish pedigree 110. these results, together with previous negative linkage findings, suggest that there is not one single gene on 11p conferring susceptibility for bipolar affective disorders among the old order amish. | 1991 | 1774072 |
| lactoferrin levels and unsaturated iron-binding capacity in colostrum of brazilian women of two socioeconomic levels. | the objective of this study was to compare lactoferrin (lf) levels and unsaturated iron-binding capacity (uibc) of colostrum from brazilian women aged 20-38 years of low (n = 28) and high (n = 15) socioeconomic level. both groups received a similar amount of supplemental iron (25-65 mg/day) during pregnancy. we also determined the relationship between lf levels and uibc, and between these components and maternal iron status. both groups (low and high socioeconomic level) showed adequate and simi ... | 1991 | 1797281 |
| lactoferrin levels and unsaturated iron-binding capacity in colostrum of brazilian women of two socioeconomic levels. | the objective of this study was to compare lactoferrin (lf) levels and unsaturated iron-binding capacity (uibc) of colostrum from brazilian women aged 20-38 years of low (n = 28) and high (n = 15) socioeconomic level. both groups received a similar amount of supplemental iron (25-65 mg/day) during pregnancy. we also determined the relationship between lf levels and uibc, and between these components and maternal iron status. both groups (low and high socioeconomic level) showed adequate and simi ... | 1991 | 1797281 |
| cloning, expression and sequence analysis of the gene encoding the 120 kda surface-exposed protein of rickettsia rickettsii. | 1991 | 1809847 | |
| [anti-inflammatory activities and effect of rhizoma alismatis on immune system]. | 10 and 20 g/kg ig of rhizoma alismatis (ra) markedly decrease the clearance rate of charcoal particles in mice, but have no significant effect on the weight of immune organs, or on the content of serum antibody hemolysin and immunoglobulin g and the delayed footpad edema induced by sheep red blood cell. the contact dermatitis of mouse pinnae immunized with dinitrochlorobenzene is inhibited by ra when it is given before challenge. in addition, ra 20g/kg suppresses the swelling of mouse rinnae ind ... | 1991 | 1820797 |
| rickettsia rickettsii infection of cultured endothelial cells induces release of large von willebrand factor multimers from weibel-palade bodies. | the clinical manifestations of rocky mountain spotted fever (rmsf) result from rickettsia rickettsii (r rickettsii) infection of endothelial cells and are mediated by pathologic changes localized to the vessel, including in situ thrombosis and tissue ischemia. this study uses in vitro infection of cultured human umbilical vein endothelial cells with r rickettsii to test the hypothesis that such infection induces von willebrand factor (vwf) release from weibel-palade bodies, a process that could ... | 1991 | 1824255 |
| effects of venous hypertension on rabbit free flap survival. | a two-stage project was developed to study the effect of increased venous pressure on blood flow and survival in microvascular free tissue transfers. a rabbit epigastric fasciocutaneous free flap model was used. the blood flow in the rabbit epigastric free flap is 1.07 +/- 0.06 ml/min. the average venous pressure is 8.6 +/- 1.7 cm water. a logarithmic relationship between blood flow and venous pressure was demonstrated, with a statistically significant decrease in blood flow to 20-35% of the con ... | 1991 | 1895934 |
| flinders island spotted fever: a newly recognised endemic focus of tick typhus in bass strait. part 2. serological investigations. | twenty-six cases of a spotted-fever-like illness have been identified on flinders island, tasmania, over a 17 year period. these patients and 335 healthy persons from the island were investigated serologically using the weil-felix agglutination test (proteus sp. antigens ox2, ox19, oxk) and rickettsia-specific microimmunofluorescence. the antigens used in these latter tests comprised one member of the typhus group (rickettsia typhi) and three members of the spotted fever group (rickettsia ricket ... | 1991 | 1898756 |
| fatal rocky mountain spotted fever in maryland--1901. | 1991 | 1899275 | |
| in vitro susceptibilities of coxiella burnetii, rickettsia rickettsii, and rickettsia conorii to the fluoroquinolone sparfloxacin. | in vitro susceptibilities of rickettsia rickettsii, rickettsia conorii, and coxiella burnetii to the new fluoroquinolone sparfloxacin (at-4140; rp 64206) were determined. plaque and dye uptake assays were used to measure the mics against r. rickettsii and r. conorii. the susceptibilities of c. burnetii nine mile and q 212 were determined in two acute-infection models and in two chronic-infection models. the mics were 0.125 to 0.25 microgram/ml for r. rickettsii and 0.25 to 0.5 microgram/ml for r ... | 1991 | 1901703 |
| fine needle aspiration biopsy diagnosis of tuberculous lymphadenitis in patients with and without the acquired immune deficiency syndrome. | all bellevue hospital cases from a recent 27-month period whose fine needle aspiration (fna) samples of cervical or supraclavicular masses showed acute and/or granulomatous inflammation were reviewed. the 30 patients included 8 with the acquired immune deficiency syndrome (aids), 3 with the aids-related complex (arc), 2 with aids risk factors and 17 without known risk factors for aids. of these, mycobacterial infections had been diagnosed in 22 patients: 18 by cultures positive for mycobacterium ... | 1991 | 1904179 |
| rocky mountain spotted fever. | rocky mountain spotted fever, the most prevalent rickettsial disease in the united states, is one of the most severe of all infectious diseases. it remains an important infectious disease because of its prevalence, the difficulty of clinical diagnosis, potentially fatal outcome, and lack of a widely available sensitive and specific diagnostic test during the acute stage of the illness. | 1991 | 1904897 |
| dna base composition of rickettsia tsutsugamushi determined by reversed-phase high-performance liquid chromatography. | the dna base composition of rickettsia tsutsugamushi was determined by reversed-phase high-performance liquid chromatography and compared with that of rickettsia rickettsii. the g+c contents were 28.1 to 30.5 mol% for r. tsutsugamushi and 32.1 mol% for r. rickettsii. | 1991 | 1906730 |
| [gas chromatograms of fatty acids from rickettsia and their similarities]. | fatty acid compositions of 7 strains of rickettsia were analyzed by a on-line gc/ms system. these strains were r. prowazekii e, r. conorii simko, r. rickettsii r, r. sibirica barbash and 246, r. sinkiangensis jinghe, and r. heilungkiangensis 54. the samples were purified by means of the concentrated salt-ether method. there were about 50 peaks in the fatty acid profiles, and 16 of these peaks were determined, i.e. c22:0, c19:0, c18:0, c18:1, c18:2, c17:0, c16:0, c16:1, 3-oh-c14:0, c15:0, c14:0, ... | 1991 | 1907779 |
| some contributions of electron microscopy to the study of the rickettsiae. | electron microscopy has provided valuable insights into the study of rickettsiae as intracellular parasites from several important perspectives. this tool has allowed researchers to delineate the fine structural features of these organisms and to show that they truly resemble free-living bacteria. furthermore, it has been shown that there are subtle, but distinct differences in the outer envelope structure of some members of the genus rickettsia that may explain reported differences in tinctoria ... | 1991 | 1909242 |
| antigenic relationships among the rickettsiae of the spotted fever and typhus groups. | using immunoblots to analyze antigenic relationships among the pathogenic spotted fever and typhus group rickettsiae, i found that the rickettsial lipopolysaccharide (lps) was a group-specific antigen. all the rickettsiae examined had 135-kda and 58-kda protein antigens. the spotted fever rickettsiae and rickettsia canada had, in addition, 190-kda protein antigens which were antigenic analogs of previously described protective antigens of r. conorii and r. rickettsii. | 1991 | 1916232 |
| heparin protects human endothelial cells infected by rickettsia rickettsii. | routine culture of endothelial cells currently includes the use of heparin, which significantly reduces cell doubling time and increases cell population size. heparin protects cultured arterial endothelial cells from damage by toxic oxygen metabolites produced by the action of xanthine and xanthine oxidase. because of our hypothesis implicating free radicals in cell injury caused by rickettsia rickettsii, we have carried out a series of experiments to examine the effects of heparin on injury to ... | 1991 | 1937810 |
| site and strand specificity of uvb mutagenesis in the sup4-0 gene of yeast. | 1991 | 2000411 | |
| false-positive results in serologic tests for rocky mountain spotted fever during pregnancy. | the data from this study demonstrate that false-positive results from tests for rocky mountain spotted fever increase with the duration of pregnancy. the sera of 4.0% (2/50) of women in their first trimester of pregnancy, 10.9% (5/46) in their second trimester, and 12.1% (12/99) in their third trimester yielded false-positive results from latex agglutination assays for rickettsia rickettsii infections. the cause of these false-positive results was not determined by this study. these false-positi ... | 1991 | 2000516 |
| rocky mountain spotted fever. | rocky mountain spotted fever is an endemic tickborne disease found throughout the united states and other regions of the world. exposure may result in a spectrum of disease from subclinical infection to severe or fatal multiorgan collapse. the disease is maintained in nature in ixodid tick vectors and their hosts. the most important ticks in the united states are dermacentor variabilis and dermacentor andersoni. small mammals are the natural reservoirs in the wild. dogs become infected when a ti ... | 1991 | 2014623 |
| effects of amino acid infusion on renal hemodynamics. role of endothelium-derived relaxing factor. | ingestion of protein or intravenous infusion of amino acids acutely elevates glomerular filtration rate (gfr) and renal plasma flow (rpf) by unknown mechanisms. endothelium-derived relaxing factor (edrf), now known to be nitric oxide derived from metabolism of l-arginine, participates in local regulation of vascular tone. to investigate the hypothesis that edrf may participate in the renal vasodilatation and increased gfr after amino acid infusion, we characterized the effect of inhibition of ed ... | 1991 | 2045148 |
| comparative susceptibility to mouse interferons of rickettsia tsutsugamushi strains with different virulence in mice and of rickettsia rickettsii. | three strains of rickettsia tsutsugamushi (karp, gilliam, and ta716, representing three virulence types in mice) were examined for their sensitivity to the inhibitory effects of recombinant gamma interferon (ifn-gamma) and purified ifn-alpha/beta in two cultured mouse fibroblast cell lines. the susceptibilities of another species, rickettsia rickettsii, and of encephalomyocarditis virus (emcv) were also tested for comparative purposes. ifn-gamma inhibited rickettsial replication in only one of t ... | 1991 | 1718869 |
| the 120 kilodalton outer membrane protein (romp b) of rickettsia rickettsii is encoded by an unusually long open reading frame: evidence for protein processing from a large precursor. | a rickettsia rickettsii outer surface membrane protein (romp b), of an apparent molecular mass of 120 kilodaltons, is a major surface antigen of the rickettsiae that displays genus, species, and sub-species specific antigenic determinants. the 5' portion of this gene was found to be unstable in plasmids, but was stably cloned in a lambda vector. the nucleotide sequence of the 5' terminus has been determined, thus completing the dna sequence of the entire gene. genetic analysis revealed an unusua ... | 1991 | 1724278 |
| evidence for proteolytic cleavage of the 120-kilodalton outer membrane protein of rickettsiae: identification of an avirulent mutant deficient in processing. | the 120-kda rickettsial outer membrane protein (rompb) is encoded by a gene with the capacity to encode a protein of approximately 168 kda. the carboxy-terminal end of the molecule is apparently cleaved to yield 120- and 32-kda products. both polypeptides are surface exposed and remain associated with the outer membrane of intact rickettsiae. all species of rickettsiae examined display similar cleavage of rompb. comparison of diverse species of rickettsiae demonstrate a conserved n terminus of t ... | 1992 | 1729180 |
| [spotted fever: isolation of rickettsia from a skin biopsy sample]. | a 2 years old child living in an area of the state of são paulo, known in the past as endemic for rickettsiosis developed clinical evidences of spotted fever after a tick bite. rickettsiae were isolated from guinea pigs inoculated with a skin homogenate. in sera tested by indirect immunofluorescence with rickettsia rickettsii standard antigen, igg specific antibody titers raised from 1:512 in the first sample to 1:2048 in the third one; igm specific antibody titer was 1:128 in the three samples. ... | 1992 | 1307409 |
| determination of genome sizes of rickettsia spp. within the spotted fever group, using pulsed-field gel electrophoresis. | the chromosome lengths of six spotted fever group rickettsia species (rickettsia rickettsii, r. conorii, r. rhipicephali, r. sibirica, r. australis, and r. akari) were estimated by pulsed-field gel electrophoresis. the genome size of r. rickettsii was about 2,100 kb, but the chromosome lengths of the five other species were, surprisingly, much lower and ranged between 1,200 and 1,300 kb. | 1992 | 1331027 |
| rickettsia rickettsii induces superoxide radical and superoxide dismutase in human endothelial cells. | human endothelial cells infected with rickettsia rickettsii, the etiological agent of rocky mountain spotted fever, undergo striking morphological changes to the endoplasmic reticulum-outer nuclear envelope complex. these changes are accompanied by concurrent accumulation of intracellular peroxides. both of these findings are consistent with the notion that cells undergo some form of oxidative stress. since oxidant injury is often initiated or mediated through oxygen radicals, we examined supero ... | 1992 | 1333449 |
| susceptibility of rickettsia conorii, r. rickettsii, and coxiella burnetii to pd 127,391, pd 131,628, pefloxacin, ofloxacin, and ciprofloxacin. | plaque formation and dye uptake assays were used to measure the mics of pd 127,391 and pd 131,628 against rickettsia species. the mics of pd 127,391 were 0.25 microgram/ml for rickettsia rickettsii and 0.125 to 0.25 microgram/ml for rickettsia conorii. the mics of pd 131,628 were 0.25 to 0.5 microgram/ml for r. rickettsii and 0.5 microgram/ml for r. conorii. as determined by the shell vial technique, 15 strains of coxiella burnetii were susceptible to pd 127,391 and pd 131,628 (mic, < or = 1 mic ... | 1992 | 1336950 |
| african tick-bite fever: a new spotted fever group rickettsiosis under an old name. | 1992 | 1357393 | |
| specificity of a tumor marker (ca54/61) and its individual epitopes recognized by monoclonal antibodies, ma54 and ma61, in human tumor patients. | the specificity of a tumor marker (ca54/61) and its individual epitopes (ca54 and ca61) recognized by monoclonal antibodies (ma54 and ma61) and expressed by the same tumor marker were studied. serum levels of ca54 and ca61 were compared with that of ca54/61. in lung adenocarcinoma and ovarian carcinoma, the positive rates of ca61 (42% and 68%) were higher than those of ca54 (32% and 32%) and similar to those of ca54/61 (45% and 74%). the serum levels of ca54 and ca61 showed a significant correla ... | 1992 | 1383482 |
| a cluster of tick-borne infections: association with military training and asymptomatic infections due to rickettsia rickettsii. | during the spring of 1989, 86 members of a military unit from the state of maryland, usa, participated in two-week-long training manoeuvres in the states of arkansas (location fc) and virginia (location faph). acute febrile illnesses due to infections with two tick-borne pathogens, rickettsia rickettsii and ehrlichia sp., were confirmed serologically in 2 initial cases who were admitted to the hospital. a seroepidemiological investigation among unit members found an additional 17 of 109 individu ... | 1992 | 1412666 |
| demonstration and partial characterization of antigens of rickettsia rhipicephali that induce cross-reactive cellular and humoral immune responses to rickettsia rickettsii. | the relatively unrelated spotted fever group rickettsia rickettsia rhipicephali conferred on guinea pigs protective immunity against challenge with virulent r. rickettsii. immunity was conferred at all doses of r. rhipicephali used in the study. because of the serologic unrelatedness of these two rickettsiae, determined by the use of microimmunofluorescence and other serological assays, further studies were performed to define the nature of the immune response elicited by r. rhipicephali and the ... | 1992 | 1452343 |
| characterization and comparison of australian human spotted fever group rickettsiae. | the microbiological and molecular characteristics of the rickettsiae isolated from humans with queensland tick typhus (qtt) caused by rickettsia australis and the recently described flinders island spotted fever (fisf) were compared. clinically and serologically, the diseases are similar. cell culture reveals differences in the plaque-forming abilities of the isolates. characterization of the gene encoding the genus-specific 17-kda antigen of r. australis revealed a unique nucleotide sequence un ... | 1992 | 1452660 |
| rocky mountain "spotless" and "almost spotless" fever: a wolf in sheep's clothing. | in 10 (10.8%) of 93 laboratory-confirmed or probable cases of rocky mountain spotted fever seen at duke university medical center from 1969 to 1991, illness without rash or fleeting or atypical skin eruptions were noted. data from these 10 cases and 33 similar previously reported cases of rocky mountain "spotless" or "almost spotless" fever support the premise that human rickettsia rickettsii infection has a broader spectrum than that indicated by its classic description. sixty-one percent of pa ... | 1992 | 1520791 |
| detection of rickettsia rickettsii in saliva, hemolymph and triturated tissues of infected dermacentor andersoni ticks by polymerase chain reaction. | the technique of polymerase chain reaction (pcr) is potentially superior to existing methods for detecting rickettsial infections in ticks. for this reason, we developed assays for identifying rickettsial infections in ticks by pcr. our assays amplified a 500 bp fragment from the gene encoding the romp b protein of rickettsia rickettsii. the selected primers amplified fragments of the predicted size from all spotted fever group rickettsiae (r. rickettsii, r. parkeri, r. conorii, r. sibirica) tes ... | 1992 | 1528203 |
| analysis of functional domains of endoglucanases from clostridium cellulovorans by gene cloning, nucleotide sequencing and chimeric protein construction. | the nucleotide sequence of engd, an endo-beta-1,4-glucanase gene from clostridium cellulovorans was determined (genbank accession no. m37434). the cooh-terminal part of the gene product, engd, contained a thr-thr-pro repeated sequence followed by a region that has homology to the exoglucanase of cellulomonas fimi. engd and engb, another c. cellulovorans endoglucanase, show 75% amino acid sequence homology at their nh2-termini, in contrast to their carboxyterminal domains which show no homology. ... | 1992 | 1538700 |
| cell-mediated immune responses of adults to vaccination, challenge with rickettsia rickettsii, or both. | as a part of a study to evaluate a formalin-killed rickettsia rickettsii vaccine, lymphoproliferative (lt) and delayed-type hypersensitivity (dth) skin test responses to killed r. rickettsii were measured as correlates of cell-mediated immunity in volunteers who were vaccinated, challenged with r. rickettsii, or both. we detected lt responses in 26 (51%) of 51 volunteers after vaccination. after challenge, six of six unvaccinated volunteers and 12 of 16 vaccinated volunteers developed rocky moun ... | 1992 | 1539743 |
| analysis of leptospira spp., leptonema illini, and rickettsia rickettsii for the 39-kilodalton antigen (p39) of borrelia burgdorferi. | five serovars of leptospira interrogans, leptospira biflexa, leptonema illini, and rickettsia rickettsii were examined and found not to contain the 39-kda antigen (p39) of borrelia burgdorferi, the lyme disease spirochete. the specificity of this antigen and its reactivity with human lyme disease sera should exclude the possibility of false-positive serum samples from patients having had either leptospirosis or rocky mountain spotted fever, as well as tick-borne relapsing fever and syphilis, as ... | 1992 | 1551994 |
| synthetic cephalosporins. viii. synthesis and antibacterial activity of cp6232, a new anti-pseudomonal cephalosporin. | 1992 | 1592693 | |
| spotted fever rickettsiae in ticks from the northern sinai governate, egypt. | a field study was initiated in 1988 to investigate whether spotted fever group rickettsiae occur in geographic areas in egypt that are adjacent to an area in the southern israeli negev that has a defined focus of spotted fever disease. ticks were collected from dogs, sheep, and camels at four study sites in the northern sinai. tick hemolymph was processed for rickettsial detection by staining with fluorescein isothiocyanate-conjugated antibody to rickettsia rickettsii. of the 442 hemolymphs exam ... | 1992 | 1599048 |
| gelatin particle agglutination test for early serodiagnosis of japanese spotted fever. | a gelatin particle agglutination (pa) test for japanese spotted fever has been developed. gelatin particles were sensitized with a sonicated causative rickettsia and used as antigens. the antibodies by pa test were detected as early as days 4-7 after the onset, whereas those by indirect immunoperoxidase (ip) test were detected after days 8-11. in addition, pa titers were higher than ip titers before days 20-23. the agglutinins detected by pa test were proven to be igm because they were all sensi ... | 1992 | 1603001 |
| ultrastructural study of the response of cells infected in vitro with causative agent of spotted fever group rickettsiosis in japan. | the response of host cells l929 infected with causative agent of spotted fever group (sfg) rickettsiosis in japan, the katayama strain, was studied by electron microscopy. the rickettsiae penetrated the cytoplasm and multiplied here and after prolonged incubation progressed into the dilated cisternae of rough endoplasmic reticulum (rer), the perinuclear space, the deep invaginated nuclear membrane, and then the nucleoplasm of the host cells. the intranuclear rickettsiae showed different states: ... | 1992 | 1610553 |
| penetration of host cells by rickettsia rickettsii appears to be mediated by a phospholipase of rickettsial origin. | internalization of obligate intracellular bacteria belonging to the genus rickettsia by eukaryotic cells requires participation of both the parasitized host and the microorganism. the term "induced phagocytosis" has been used specifically to describe the entry of rickettsia prowazekii, although a similar mechanism is likely for r. rickettsii. a role for a phospholipase in the internalization process has been proposed for both of these organisms, with the strongest supporting evidence provided fo ... | 1992 | 1612741 |
| increase in globin chains and globin mrna in erythroleukemia cells in response to hemin. | 1992 | 265145 | |
| feelings, feelings: how to make a rational response to emotional behavior. | 1992 | 258283 | |
| mycotoxins in soy sauce and fermented soybean paste of taiwan. | 1992 | 270549 | |
| why do nurses go "off sick"? | 1992 | 257228 | |
| nursing staff deployment. | 1992 | 257517 | |
| factors associated with planned and unplanned nuptial births. | the incidence of unplanned pregnancy in a cohort of 1038 mothers of nuptial children was examined. the major findings were: (1) about one third of the pregnancies were unplanned but there was little evidence that unplanned children were unwanted; (2) there was no evidence to suggest that unplanned birth was related to poor education, low socio-economic status or lack of knowledge of contraception; (3) 40 percent of unplanned births were the result of contraceptive failure; (4) a common denominat ... | 1993 | 280785 |
| comparison of the rompa gene repeat regions of rickettsiae reveals species-specific arrangements of individual repeating units. | genes containing repeating units have been described for several eukaryotic and prokaryotic proteins, although none exhibit the extraordinary length and conservation seen with the rickettsia rickettsii 190-kda protein (ompa) repeat region. the genetic organization of the repeat regions of the ompa gene for r. conorii and r. akari was examined and compared to that of r. rickettsii. individual repeating units, which constitute the makeup of the repeat regions, share extensive dna homology among th ... | 1993 | 7680636 |
| e-selectin-dependent neutrophil adhesion to rickettsia rickettsii-infected endothelial cells. | increased neutrophil or hl60 cell adhesion to rickettsia rickettsii-infected endothelial cells (ecs) was observed at 6 to 8 hours after the initiation of infection, diminishing by 24 hours. similar increases were observed using formaldehyde-fixed neutrophils. cellular association and likely the intracellular presence of rickettsiae was required for enhanced neutrophil adhesion, because culture medium conditioned by infected cells or rickettsiae rendered noninfective by pretreatment with tetracyc ... | 1993 | 7683219 |
| eia with species-specific monoclonal antibodies: a novel seroepidemiologic tool for determination of the etiologic agent of spotted fever rickettsiosis. | all currently available assays for antibodies to the etiologic agents of spotted fever group rickettsioses detect reactivity with antigens of lipopolysaccharides and the major cell wall proteins, which contain epitopes that are shared among many spotted fever group rickettsiae. the hypothesis of this study was that a monoclonal antibody to a species-specific epitope of rickettsia rickettsii would be blocked from binding to the rickettsial surface if the rickettsiae were incubated previously with ... | 1993 | 7693827 |
| preliminary evaluation of the indx dip-s-ticks with positive rickettsial samples in malaysia. | forty-four serum samples of various reactivities to rickettsial antigens demonstrated by the indirect immunoperoxidase technique were tested with indx dip-s-ticks (indx integrated diagnostics inc., usa) kit for the detection of tick borne diseases. the kit utilised rickettsia rickettsii the causative agent of rocky mountain spotted fever (rmsf) as antigens. the samples positive for endemic typhus were also tested against r. typhi, the agent for endemic typhus by the same method. the aim of this ... | 1993 | 8277794 |
| human endothelial cells as an alternative to dh82 cells for isolation of ehrlichia chaffeensis, e. canis, and rickettsia rickettsii. | ehrlichia chaffeensis, etiologic agent of human ehrlichiosis, and rickettsia rickettsii, etiologic agent of rocky mountain spotted fever (rmsf), are both tick-borne agents that cause nonspecific symptoms that may be indistinguishable from each other early in the course of infection. e. canis is a canine pathogen closely related to e. chaffeensis and was initially suspected of being the causative agent of human ehrlichiosis. if a febrile illness is reported, after tick exposure, neither ehrlichio ... | 1993 | 8297496 |
| brazilian spotted fever in espirito santo, brazil: description of a focus of infection in a new endemic region. | although brazilian spotted fever is known to occur in several adjacent states, infection with spotted fever group (sfg) rickettsiae has not previously been documented in the brazilian state of espirito santo. we report a cluster of two proven and four suspected cases of brazilian spotted fever in a small valley near colatina, espirito santo. four patients died without confirmatory serologic or pathologic studies. the two surviving patients had serologic and/or pathologic evidence of infection wi ... | 1993 | 8357085 |
| comparison of latex agglutination, indirect immunofluorescent antibody, and enzyme immunoassay methods for serodiagnosis of rocky mountain spotted fever in dogs. | indirect immunofluorescent antibody (ifa), latex agglutination (la), and enzyme immunoassay (eia) methods were compared for evaluation of the serum antibody responses of dogs experimentally and naturally exposed to spotted fever-group rickettsiae. selected sera (obtained on days 1, 42, 53, 124, 145, 236, 255, 264, and 292) were examined from three 8-month-old female beagles inoculated with rickettsia rickettsii on days 34 and 250 of the study. a second group of dogs comprised three 8-month-old f ... | 1993 | 8427468 |
| directional actin polymerization associated with spotted fever group rickettsia infection of vero cells. | members of the spotted fever group (sfg) of rickettsiae spread rapidly from cell to cell by an unknown mechanism(s). staining of rickettsia rickettsii-infected vero cells with rhodamine phalloidin demonstrated unique actin filaments associated with one pole of intracellular rickettsiae. f-actin tails greater than 70 microns in length were seen extending from rickettsiae. treatment of infected cells with chloramphenicol eliminated rickettsia-associated f-actin tails, suggesting that de novo prote ... | 1993 | 8478082 |
| rocky mountain spotted fever complicated by gangrene: report of six cases and review. | although mortality due to fulminant rocky mountain spotted fever (rmsf) is well appreciated, the ability of the disease to cause survivors to become permanently disabled is not as widely known. we report six cases of rmsf complicated by gangrene. although four patients required multiple limb and/or digital amputations, only one death resulted. our review of the english-language literature revealed 23 additional cases of rmsf complicated by gangrene. pathophysiologically, gangrene is most likely ... | 1993 | 8507753 |
| differentiation of rickettsia japonica by restriction endonuclease fragment length polymorphism using products of polymerase chain reaction amplification with rickettsia rickettsii 190-kilodalton surface antigen gene primers. | restriction fragment length polymorphism of polymerase chain reaction (pcr) amplification products differentiated rickettsia japonica, a causative agent of oriental spotted fever, from other spotted fever group (sfg) rickettsiae. primer pair rr190. 70p and rr190. 602n of r. rickettsii 190-kda antigen gene sequence primed genomic dnas obtained from r. japonica, type strain yh and strains nt, nk, yki, and tkn. the products were cleaved by psti but not by afai restriction endonuclease. the psti dig ... | 1993 | 7901739 |
| in vitro susceptibilities of spotted fever group rickettsiae and coxiella burnetti to clarithromycin. | the in vitro bacteriostatic activity of clarithromycin, a new macrolide derivative, against rickettsia rickettsii, rickettsia conorii, and "rickettsia israeli" was determined by the plaque assay and the dye uptake assay. both bacteriostatic and bactericidal activities of clarithromycin against the nine mile, q212, priscilla, and me9 strains of coxiella burnetti were evaluated by using three cell culture systems. clarithromycin showed improved antibacterial activity compared with that of erythrom ... | 1993 | 8109928 |
| rickettsia rickettsii infection of cultured human endothelial cells induces tissue factor expression. | microvascular thrombi underlie many of the clinical manifestations of rocky mountain spotted fever (rmsf), a disease characterized by rickettsia rickettsii infection of vascular endothelial cells. studies were designed to determine whether r rickettsii-infection of cultured human umbilical vein endothelial cells results in tissue factor (tf) induction, a process that could directly activate coagulation in infected vessels. whereas uninfected endothelial cell cultures showed essentially undetecta ... | 1994 | 8123842 |
| characterization of a rickettsia rickettsii dna fragment analogous to the fir a-orf17-lpxa region of escherichia coli. | the fira and lpxa genes, as well as an orf coding for a putative 16-kda protein of unknown function, have been identified and characterized in the obligate intracellular bacterium. rickettsia rickettsii. this is the first description of these genes, which code for enzymes involved in the biosynthesis of lipid a, in a species outside of the enterobacteriaceae. the deduced amino acid (aa) sequences of fira, orf16 and lpxa of r. rickettsii, when compared to their escherichia coli analogs, exhibited ... | 1994 | 8125326 |
| infection of human endothelial cells by rickettsia rickettsii causes a significant reduction in the levels of key enzymes involved in protection against oxidative injury. | the activities of glucose-6-phosphate dehydrogenase, catalase, and glutathione peroxidase were significantly decreased in human endothelial cells infected with the obligate intracellular bacterium rickettsia rickettsii, the causative agent of rocky mountain spotted fever. this observation lends additional support to our hypothesis implicating oxidative damage in endothelial cell injury caused by this microorganism. | 1994 | 8188386 |
| evaluation of immunoperoxidase techniques to detect rickettsia rickettsii in fixed tissue sections. | standard immunoperoxidase techniques were evaluated in the diagnosis of rocky mountain spotted fever (rmsf). formalin-fixed, paraffin-embedded tissue was tested to detect rickettsia rickettsii using the same antibody provided by the centers for disease control and prevention that is used for direct immunofluorescence (dif). tissues from 23 patients with suspected rmsf were divided: some were snap-frozen for dif; the remainder were fixed in formalin, processed routinely, and embedded in paraffin ... | 1994 | 8209863 |
| dna typing of rickettsiae in naturally infected ticks using a polymerase chain reaction/restriction fragment length polymorphism system. | we used the polymerase chain reaction/restriction fragment length polymorphism (pcr/rflp) rickettsial typing system of regnery and others to rapidly identify rickettsiae in naturally infected ticks. unlike previously described methods, our pcr assays type rickettsiae directly from tick tissues without first isolating the organisms. we collected 226 adult dermacentor andersoni ticks in the bitterroot mountains of western montana and analyzed them for possible rickettsial infection by hemolymph te ... | 1994 | 7906924 |
| identification of rickettsia conorii infection by polymerase chain reaction in a soldier returning from somalia. | a soldier developed characteristic manifestations of boutonneuse fever shortly after leaving somalia. rickettsial dna was detected in a biopsy sample of the tache noire by a polymerase chain reaction (pcr) in which primers derived from the 190-kd antigen gene of rickettsia rickettsii were used. the source of this dna was identified as rickettsia conorii by restriction fragment length polymorphism (rflp) analysis of the pcr product. r. conorii was also isolated from the skin biopsy specimen. the ... | 1994 | 7948564 |
| rickettsialpox in a new york city hospital, 1980 to 1989. | rickettsialpox is caused by rickettsia akari, which is transmitted from rodents to humans by bloodsucking mites. the initial skin lesion forms an eschar and is followed by the development of fever, malaise, myalgia, and 5 to 40 maculopapules and papulovesicles. the disease, which responds to tetracycline, can be mistaken for chickenpox. the diagnosis has been based on an increase in serum antibody titers against r. akari over a period of three to eight weeks. we discuss a more rapid technique th ... | 1994 | 7969341 |
| ancestral divergence of rickettsia bellii from the spotted fever and typhus groups of rickettsia and antiquity of the genus rickettsia. | the eubacterial genus rickettsia belongs to the alpha subgroup of the phylum proteobacteria. this genus is usually divided into three biotypes on the basis of vector host and antigenic cross-reactivity characteristics. however, the species rickettsia bellii does not fit into this classification scheme; this organism has characteristics common to both the spotted fever group and the typhus group biotypes and also exhibits some unique features. sequences of the 16s rrna and 23s rrna genes from ric ... | 1994 | 7981106 |
| sequence analysis of the sbsa gene encoding the 130-kda surface-layer protein of bacillus stearothermophilus strain pv72. | bacillus stearothermophilus (bs) contains a surface-layer (s-layer) protein (sbsa), which forms a hexagonal array on the cell wall. in order to understand the structural/functional relationship of sbsa from bs pv72, the entire nucleotide (nt) sequence of the sbsa gene was determined from three overlapping fragments. the 3'-end was cloned and expressed in escherichia coli, whereas the 5'-region was amplified from the genome of bs pv72 by the polymerase chain reaction using two overlapping fragmen ... | 1994 | 8045409 |
| species-specific balb/c mouse antibodies to rickettsiae studied by western blotting. | balb/c mice were inoculated intraperitoneally either once only, or up to four times at weekly intervals, with viable rickettsia rickettsii, rickettsia conorii or the israeli spotted fever group rickettsia. sera collected one week after the last inoculation were tested for the presence of antibodies reactive with the above organisms by indirect fluorescent antibody testing and western blot. with repeated inoculations there was a general progressive rise in homologous and heterologous immunofluore ... | 1994 | 8050715 |
| rocky mountain spotted fever in an endemic area in minas gerais, brazil. | only one species of spotted fever-group rickettsiae that is pathogenic for humans has been isolated in brazil, where few physicians are familiar with this disease. in order to obtain information on tick-borne rickettsiosis, a study was performed in the county of santa cruz do escalvado, state of minas gerais, brazil, where a fatal clinical case confirmed by specific immunofluorescence had been reported. serum samples obtained from 679 humans and 96 dogs were tested by indirect immunofluorescence ... | 1994 | 8524052 |
| the use of a polymerase chain reaction as a diagnostic test for rocky mountain spotted fever. | a polymerase chain reaction (pcr) for amplifying ribosomal dna of rickettsia rickettsii was performed on blood clots and urine samples from 10 patients with suspected rocky mountain spotted fever (rmsf) and five controls with nonrickettsial diseases. the results of this pcr-based procedure were positive in four of the five patients with probable rmsf, but reamplification was required in three patients. rickettsia rickettsii was grown from the blood of two of these four patients. the urine from o ... | 1994 | 8304573 |
| granulocytic ehrlichiosis and meningitis in a dog. | a 4-year-old male mixed-breed dog from the sierra nevada mountains in california was referred because of epistaxis and signs of cervical pain. dermacentor variabilis ticks were found on the dog at the time of physical examination. clinicopathologic abnormalities included nonregenerative anemia, thrombocytopenia, and rare intracytoplasmic morulae within circulating neutrophils. abnormalities of the csf included pleocytosis and intracytoplasmic morulae in approximately 9% of neutrophils. serum ant ... | 1994 | 7730122 |
| genomic identification of rickettsia slovaca among spotted fever group rickettsia isolates from dermacentor marginatus in armenia. | restriction fragment length polymorphism (rflp) analysis of polymerase chain reaction (pcr) amplified genes was used for genomic identification of armenian isolates of the spotted fever group (sfg) rickettsiae with unclear taxonomic position. analysis was performed by using one genus-specific primer pair derived from r. prowazekii citrate synthase gene and two species-specific primer pairs derived from r. rickettsii genes for 190 k and 120 k antigens following alui, psti and rsai digestion of am ... | 1994 | 7793355 |
| detection of dna of causative agent of spotted fever group rickettsiosis in japan from the patient's blood sample by polymerase chain reaction. | the polymerase chain reaction (pcr) was applied for the etiological diagnosis of spotted fever group (sfg) rickettsiosis in japan. nucleotide primers derived from the 17-kda antigen gene of rickettsia rickettsii primed a rickettsia-specific 246-base-pair product for all of the katayama, abe, misaka and kojima strains, which we had isolated previously. moreover, we were able to detect the same product by pcr amplification from the peripheral blood of a patient in the acute stage of the illness. t ... | 1994 | 7799840 |
| differential activity of rickettsia rickettsii opma and ompb promoter regions in a heterologous reporter gene system. | the outer membrane of the gram-negative obligate intracellular parasite rickettsia rickettsii contains two large surface protein antigens with approximate molecular masses of 200 and 135 kda termed rompa and rompb, respectively. rompb is the most abundant protein in the outer membrane, while rompa is a relatively minor constituent. densitometry of intrinsically radiolabelled protein profiles from r. rickettsii-infected vero cells indicated a molar ratio of approximately 1:9 between rompa and rom ... | 1994 | 7812435 |
| nucleotide sequence of polymerase chain reaction product amplified from rickettsia japonica dna using rickettsia rickettsii 190-kilodalton surface antigen gene primers. | the pcr product amplified from rickettsia japonica with the primer pair rr 190.70p and rr 190.602n of r. rickettsii 190-kda antigen gene was cloned into m13mp19 rf dna at the eco ri site and sequenced by chemiluminescent dna sequencing. the sequence revealed a molecular size of 533 base pairs (bp). the primer-flanking region of 491 bp, an open reading frame, was compared with the corresponding region of r. rickettsii, demonstrating 35 nucleotide substitutions in r. japonica. the sequence of prim ... | 1994 | 7898385 |
| rickettsia rickettsii has proteins with cross-reacting epitopes to eukaryotic phospholipase a2 and phospholipase c. | the entry, and possibly the exit, of rickettsiae from eukaryotic cells, as well as erythrocyte lysis by some members of this group of organisms, is thought to be mediated by a phospholipase a activity even though the enzyme has not been isolated from these organisms. evidence for phospholipase c, on the other hand, has not been reported for the genus rickettsia. in this study, in a preliminary attempt to demonstrate the presence of phospholipase a2 and phospholipase c in the virulent sheila smit ... | 1994 | 7532256 |