Publications

mouse skin damage caused by cytolysin from vibrio vulnificus and by v. vulnificus infection.	light and electron microscopy of mouse skin damage caused by intradermal infection with a virulent strain of vibrio vulnificus and by a single intradermal injection of the cytolytic toxin produced by the bacterium revealed similar structural alterations. the epidermis was intact; however, the infection and toxin produced acute cellulitis characterized by extensive extracellular edema; disorganization of collagen bundles; large accumulations of cell debris and plasma proteins; damaged or necrotic ...	1987	3805762
pneumonia--the quality of medical records data.	the quality of medical records data for patients who were hospitalized with community-acquired pneumonia was assessed by comparing medical records data with data obtained in a prospective study of pneumonia for the period april 1, 1984, to december 31, 1984. one hundred five patients fulfilled the case definition of pneumonia for entry into the prospective study. one hundred twenty-seven patients were identified by medical records data. seventy-three of the patients appeared in both studies. the ...	1987	3807447
comparison of the pharmacological properties of pituitary and biosynthetic human growth hormone. demonstration of antinatriuretic/antidiuretic and barbital sleep effects of human growth hormone in rats.	biosynthetic human growth hormone was compared with pituitary human growth hormone and pituitary 22 k in the weight gain and the tibia test. the three preparations were found to be equipotent. furthermore, the growth hormones were compared in various pharmacological test systems. all three preparations were found to have a marked antidiuretic and antinatriuretic effect in the rat and to cause a significant shortening of the hexobarbital sleeping time in mice. biosynthetic and pituitary preparati ...	1987	3811769
variation in the lymphocyte transformation assay. slope analysis of cell dose-response curves.	blood samples from a single sheep were cultured at a number of different cell concentrations and stimulated with three lymphocyte mitogens, phytohaemagglutinin, concanavalin a and pokeweed mitogen. the response to stimulation, measured as log thymidine uptake (log cpm) was found to be a linear function of log cell concentration (log cell dose). samples taken on different dates, varied significantly in the gradients of the log cpm: log cell dose plots. these alterations of gradient constituted th ...	1987	3571985
aids and non-human primates.		1987	3576926
radiosensitivity and sublethal damage repair in human umbilical cord vein endothelial cells.	the assessment of the comparative radiosensitivity of different parenchymal cells is potentially of great importance in clinical radiotherapy. previous studies from various laboratories using vessel regeneration techniques have demonstrated that endothelial cells are quite radioresistant, having a do in excess of 250 rads. the present studies were undertaken to investigate the radiosensitivity of human endothelial cells obtained from umbilical cord veins (hec) using "colony formation" as an endp ...	1987	3583859
testicular defect: the primary abnormality in gonadal dysfunction of uremia.	gonadal dysfunction is a common problem in end-stage renal disease. we decided to test the ability of the male gonads to respond to exogenous stimuli. the patients receiving chronic hemodialysis showed abnormally low levels of total testosterone and free testosterone in the presence of normal testosterone-binding globulin. furthermore, the exogenous administration of human chorionic gonadotropin failed to elicit a normal response from the male gonads of hemodialyzed patients. although basal seru ...	1987	3589761
noninvasive doppler assessment of human postprandial renal blood flow and cardiac output.	we compared the effects of protein, carbohydrate, or water ingestion on human renal blood flow (rbf) and cardiac output (co). we validated and applied a noninvasive ultrasonic doppler method to measure blood velocities and lumen diameters in the right renal artery and the ascending aorta of five healthy adults. from these measurements, we calculated the average rbf and co over five cardiac cycles. normalizing to body surface area, renal blood flow index to one kidney (ri) and cardiac index (ci) ...	1987	3591956
storage conditions and stability of thyrotropin and thyroid hormones on filter paper.	we evaluated the effects of temperature and humidity on thyroid hormones (t4, t3) and thyrotropin (tsh) measured in blood spots dried on filter paper. rias for t4 and t3 blood spots were optimized to measure these analytes over the neonatal and euthyroid adult reference intervals. sensitivities of the t3 and t4 assays were 0.5 and 10 nmol/l, respectively. a blood-spot immunoradiometric assay for tsh involving magnetizable particles was developed with a sensitivity of 6 milli-int. units/l. contro ...	1987	3594835
storage conditions and stability of thyrotropin and thyroid hormones on filter paper.	we evaluated the effects of temperature and humidity on thyroid hormones (t4, t3) and thyrotropin (tsh) measured in blood spots dried on filter paper. rias for t4 and t3 blood spots were optimized to measure these analytes over the neonatal and euthyroid adult reference intervals. sensitivities of the t3 and t4 assays were 0.5 and 10 nmol/l, respectively. a blood-spot immunoradiometric assay for tsh involving magnetizable particles was developed with a sensitivity of 6 milli-int. units/l. contro ...	1987	3594835
fibre types in extraocular muscles: a new myosin isoform in the fast fibres.	we report on the existence of a myosin heavy chain (mhc) isoform with unique structural properties in extraocular (eo) muscles. differences in mhc composition are apparent using a polyclonal antibody prepared against myosin isolated from bovine eo muscle myosin. in enzyme immunoassays and western blotting experiments, this anti-eo myosin antibody reacted specifically with the heavy chains of eo muscle myosin and not with the heavy chains of other myosins. the distribution of this new mhc isoform ...	1987	3597767
fibre types in extraocular muscles: a new myosin isoform in the fast fibres.	we report on the existence of a myosin heavy chain (mhc) isoform with unique structural properties in extraocular (eo) muscles. differences in mhc composition are apparent using a polyclonal antibody prepared against myosin isolated from bovine eo muscle myosin. in enzyme immunoassays and western blotting experiments, this anti-eo myosin antibody reacted specifically with the heavy chains of eo muscle myosin and not with the heavy chains of other myosins. the distribution of this new mhc isoform ...	1987	3597767
thin collagenous septa in cardiac muscle.	light and electron microscopy were used to study the structure and distribution of thin collagenous septa (sheets) in dog and rabbit cardiac muscle to determine whether they, like thick collagenous septa, could affect electrical impulse propagation. generally, thin septa (0.2-0.5 micron) ensheathed myocytes or groups of myocytes for short distances and thicker septa partially or completely ensheathed groups of myocytes for long distances (up to several mm); together, thin, and thick septa divide ...	1987	3605660
virulent strains of vibrio vulnificus isolated from estuaries of the united states west coast.	vibrio vulnificus was isolated from united states west coast estuaries at a low frequency (5.9%) from 529 samples of water, shellfish, and sediment. four strains tested with iron-treated mice had 50% lethal dose values ranging from 7.6 to 360 cfu, compared with a 50% lethal dose of 4.9 cfu for a clinical isolate that caused the death of a septicemic patient. the presence of this pathogen may be a hazard to users of marine beaches and consumers of raw shellfish on the west coast, especially to pe ...	1987	3606112
plasmid-mediated trimethoprim-resistance in staphylococcus aureus. characterization of the first gram-positive plasmid dihydrofolate reductase (type s1).	the trimethoprim-resistance gene located on plasmid psk1, originally identified in a multi-resistant staphylococcus aureus from australia, encodes the production of a dihydrofolate reductase (type s1), which confers a high degree of resistance to its host and is quite unlike any plasmid-encoded dihydrofolate reductase hitherto described. it has a low mr (19,700) and has a higher specific activity than the constitutive gram-negative plasmid dihydrofolate reductases. the type s1 enzyme is heat-sta ...	1987	3606580
[analysis of lethal toxin produced by vibrio vulnificus].		1987	3610014
differing resistance to polymorphonuclear cells of halophilic- and hypotonic-types of vibrio vulnificus.	bacterial growth and lethality of 4 strains of vibrio vulnificus infection of mice were enhanced by gamma-irradiation but not by treatment with carrageenan. therefore, protection against v. vulnificus, at least in the early phases, probably depends mainly on polymorphonuclear cells (pmn), since carrageenan depletes macrophages but not pmn. pmn dependent protection against infection differs for 2 types of v. vulnificus strains. halophilic- and hypotonic-type were distinguished from the correspond ...	1987	3612754
intrinsic circuitry involving the local axon collaterals of corticothalamic projection cells in mouse smi cortex.	the objective of this study was to identify the components involved in a local synaptic circuit in the mouse cerebral cortex. the local axon collaterals of corticothalamic (ct) projection cells in the posteromedial barrel subfield area of primary somatosensory cortex were labeled by the retrograde transport of horseradish peroxidase injected into the ipsilateral thalamus. thalamocortical (tc) axon terminals in the same region of cortex were labeled by lesion induced degeneration. ct axon termina ...	1987	3624546
prenatal diagnosis of minute 5p- deletion: a cytogenetic problem in detection.	an unbalanced translocation, 46,xx,der(5)t(5;11) (p15;q25)mat was ascertained by prenatal diagnosis. the mother previously had a liveborn child with cri du chat syndrome. the subtlety of the chromosome rearrangement in this case illustrates the need for the most detailed cytogenetic investigations in cri du chat cases when deletion or translocation are not immediately obvious.	1987	3627600
activation of the plasma kallikrein-kinin system by vibrio vulnificus protease.	vibrio vulnificus protease enhanced hypodermic vascular permeability when injected into the dorsal skin of a guinea pig. enhancement of permeability was observed within 2 min, and the permeability-enhancing reaction terminated at about 10 min postinjection. the permeability-enhancing reaction was greatly augmented by simultaneous injection of a kininase ii inhibitor, whereas the reaction was inhibited by soybean trypsin inhibitor, a well-known inhibitor of plasma kallikrein. furthermore, in vitr ...	1987	3649335
nonradioactive, photobiotin-labelled dna probes for the routine diagnosis of barley yellow dwarf virus.	photobiotin was used to prepare biotinylated, nonradioactive nucleic acid probes for the detection of the rna of barley yellow dwarf virus (bydv) in plant extracts. a 1.7-kb cdna of the pav isolate of bydv in the plasmid puc8 vector was biotinylated and then used intact or as sonicated double-stranded dna fragments. simple methods were developed for the preparation of partially purified nucleic acid extracts of cereals and their spotting, after formaldehyde treatment, onto nitrocellulose membran ...	1987	3654900
use of sodium dodecyl sulfate-polymyxin b-sucrose medium for isolation of vibrio vulnificus from shellfish.	the differential and selective sodium dodecyl sulfate-polymyxin b-sucrose medium (sps) of kitaura et al. (t. kitaura, s. doke, i. azuma, m. imaida, k. miyano, k. harada, and e. yabuuchii, fems microbiol. lett. 17:205-209, 1983), which highlights alkylsulfatase activity, was evaluated for its potential use in the direct isolation and enumeration of vibrio vulnificus from shellfish. v. vulnificus was detected by this method in six of nine shellfish samples collected from diverse geographic locales ...	1987	3662506
na+ modulates the k+ permeability and the membrane potential of alkalophilic bacillus.	in the absence of na+ in the medium, the membrane potential of obligately alkalophilic bacillus cells was found to be decreased by the addition of k+ to the medium, whereas k+ addition in the presence of na+ had no effect. rb+ showed essentially the same effect as k+. the decreased membrane potential was quickly restored by lowering the k+ concentration in the medium or by adding na+ or li+ to the medium. thus, in the absence of na+, the membrane potential of alkalophilic bacillus seems to be af ...	1987	3663674
vibrio vulnificus.	v vulnificus is a halophilic or salt-requiring vibrio that has been isolated repeatedly from seawater and shellfish in coastal waters. this vibrio, first described by hollis et al in 1976, can be differentiated from other similar vibrios by its ability to ferment lactose and by its lower tolerance for sodium chloride. v vulnificus, unlike most other vibrios, has seldom been incriminated as a cause of gastroenteritis but is a particularly virulent organism that causes severe wound infections in m ...	1987	3667122
new selective and differential medium for vibrio cholerae and vibrio vulnificus.	thiosulfate-citrate-bile salts-sucrose agar has been routinely used for the isolation of pathogenic vibrios, although its selectivity for vibrio cholerae and vibrio vulnificus is inadequate. therefore, a new plating medium, cellobiose-polymyxin b-colistin agar, was developed for the isolation of these two species. cellobiose-polymyxin b-colistin agar demonstrated a significant advantage over other media designed for the isolation or differentiation of vibrios: of both the 136 strains representin ...	1987	3674873
vibrio vulnificus bacteremia with endocarditis.		1987	3686154
distribution of immunoreactive inhibin like material in different regions of human prostate.	distribution of immunoreactive inhibin material (ir-ilm) in different regions of human prostate was studied by using specific radio-immunoassay. mean ir-ilm concentrations in the central, peripheral and periurethral zones were 27.3 +/- 9.3, 2.4 +/- 0.7 and 3.0 +/- 1.5 micrograms/mg protein respectively (p central vs periurethral and peripheral less than 0.05).	1987	3688474
analysis of protein patterns from different organs and cell fractions of trisomy 19 mice.	proteins were extracted from liver, brain, and skin of 6-day-old mice with trisomy (ts) 19 and fractionated into solubilized cell proteins and structure-bound cell proteins. the proteins were separated by two-dimensional electrophoresis, and protein patterns were compared in the combinations ts/normal and normal/normal. analysis of the protein patterns revealed protein spots (variants) with densities higher (h-type) or lower (l-type) in trisomies than in normal mice. some of these variants were ...	1987	3692480
effect of iron and liver injury on the pathogenesis of vibrio vulnificus.	noncholera vibrio species can cause necrotizing soft tissue infections and cellulitis in addition to gastroenteritis. cellulitis and necrotizing soft tissue infections frequently occur in people with liver disease or in people who are in some way a compromised host. we studied the effect of vibrio vulnificus on the mortality of mice treated with carbon tetrachloride, an agent that causes liver damage, desferal, an iron-chelating agent, or iron. the ld50 of v. vulnificus is approximately 2.5 x 10 ...	1987	3695454
phosphorylation, glycosylation, and proteolytic activity of the 52-kd estrogen-induced protein secreted by mcf7 cells.	we have studied the posttranslational modifications of the 52-kd protein, an estrogen-regulated autocrine mitogen secreted by several human breast cancer cells in culture (westley, b., and h. rochefort, 1980, cell, 20:353-362). the secreted 52-kd protein was found to be phosphorylated mostly (94%) on high-mannose n-linked oligosaccharide chains, and mannose-6-phosphate signals were identified. the phosphate signal was totally removed by alkaline phosphatase hydrolysis. the secreted 52-kd protein ...	1987	3543022
phosphorylation, glycosylation, and proteolytic activity of the 52-kd estrogen-induced protein secreted by mcf7 cells.	we have studied the posttranslational modifications of the 52-kd protein, an estrogen-regulated autocrine mitogen secreted by several human breast cancer cells in culture (westley, b., and h. rochefort, 1980, cell, 20:353-362). the secreted 52-kd protein was found to be phosphorylated mostly (94%) on high-mannose n-linked oligosaccharide chains, and mannose-6-phosphate signals were identified. the phosphate signal was totally removed by alkaline phosphatase hydrolysis. the secreted 52-kd protein ...	1987	3543022
the morphology and distribution of serotonin-like immunoreactive fibers in the cat dorsal column nuclei.	fibers showing serotonin-like immunoreactivity (5-ht-li) are demonstrated in the gracile, cuneate and external cuneate nuclei of the cat using avidin-biotinylated horseradish peroxidase and indirect fluorescence immunohistochemical methods. 5-ht-li fibers are located in all cytoarchitectural subdivisions of the gracile and cuneate nuclei but are restricted to the medial half of the external cuneate nucleus. in all nuclei, 5-ht-li fibers consist of long, varicose strands showing a wide range in t ...	1987	3554006
calcitonin gene-related peptide in the ventral tegmental area: selective modulation of prefrontal cortical dopamine metabolism.	the distribution of calcitonin gene-related peptide (cgrp) in the a10 dopamine (da) cell group region of the ventral tegmental area (vta) of the rat was examined using immunohistochemical techniques. cgrp-like immunoreactivity was localized to axons innervating the rostral and dorsal vta. direct administration of cgrp to the vta of the rat resulted in a dose-related increase in da utilization in the medial prefrontal cortex, but not other mesocortical, mesolimbic, or striatal da terminal field r ...	1987	3554009
effect of pre- and postnatal exposure to alcohol on perivenous and periportal neonatal rat hepatocytes.	hepatocytes from 12-day-old rats, pre and postnatally exposed to alcohol and pair-fed controls, were isolated and subfractionated in six cell subpopulations on percoll density gradients. these cells were characterized using biochemical, stereological and cytofluorometric methods. our results show that the low density cells (f2) are mainly perivenous cells, whereas the heavier cells (f6) were primarily periportal cells. these results were confirmed by alanine aminotransferase (alat), and glutamat ...	1987	3426691
vibrio vulnificus infections in singapore.		1987	3429797
liver cell lines.		1987	3431441
[hypoxia and protection of the brain (isoflurane vs halothane and enflurane].		1987	3438012
destructive chronic borrelia meningoencephalitis in a child untreated for 15 years.	a case of borrelia spirochetosis with recurring episodes of progressively destructive chronic meningoencephalitis, untreated for 15 years, is presented. the patient, now 23 years old, represents an example of the natural course of the disease and may serve as a reminder of the diagnosis in retrospective cases as well as prospectively for acute and chronic states of serous meningitis/meningoencephalitis both in children and adults.	1987	3441752
monoclonal antibodies against the haemolysin of vibrio vulnificus.	the extracellular haemolysin produced by vibrio vulnificus strain fcc was partially purified from the culture supernate by sequential ammonium sulphate precipitation, gel filtration with sepharose 4b, and deae-sephacel ion-exchange column chromatography. using this semi-purified haemolysin as the antigen, several monoclonal antibodies (mabs) were established; they were all of the igg2b class with lambda light chains. one representative mab, 6f8d, completely neutralized the haemolytic activity an ...	1987	3443854
the role of catecholamines and glucagon on serum and liver metallothionein response to restraint stress.	the role of glucagon and catecholamines on serum and liver metallothionein (mt) concentrations in basal and stress conditions has been studied. glucagon showed no effect on serum mt either in basal (unstressed) or stress (20 hours of restraint) conditions. in contrast, glucagon administration increased both unstressed and stressed levels of liver mt. no effect of the alpha + beta-catecholamine blocker labetalol on serum mt levels was observed in unstressed rats. however, the administration of la ...	1987	3448709
variability of haemolysin(s) produced by vibrio vulnificus.	the peptide composition and antigenic cross-reactivity of partially purified and concentrated haemolysins of 16 strains of vibrio vulnificus were examined by sds-page and immunoblotting analysis, using a monoclonal antibody (mab), 6f8d, raised against the haemolysin. all strains produced a common peptide of 36 kda and the mab reacted with this peptide. in some strains, larger molecules, including a 56 kda peptide, were produced, but the mab did not react with this peptide. the haemolytic activit ...	1987	3449599
mechanism of haemolysis by vibrio vulnificus haemolysin.	the haemolytic action of vibrio vulnificus haemolysin (vvh) was compared to that of streptolysin o (slo). both were cholesterol-binding haemolysins, but differed in the release of haemoglobin (hb). in the first step of haemolysis, the haemolysins were temperature-independently bound to the cholesterol site on the target erythrocyte membrane. this was followed by the rapid release of k+, which is an intra-erythrocyte marker. hb was then released, in different ways. in the case of vvh, hb was rele ...	1987	3449600
intestinal helminthiases and human health: recent advances and future needs.		1987	3294628
ca2+-binding parvalbumin in rat testis. characterization, localization, and expression during development.	parvalbumin, a ca2+-binding protein, was isolated from rat testis. this is the first demonstration of the protein in endocrine glands. by using a rat parvalbumin cdna probe, parvalbumin mrna was demonstrated in the testis, indicating that the protein is synthesized in this tissue and that testis parvalbumin is a product of the same gene as the one encoding for muscle parvalbumin. parvalbumin was localized by immunohistochemical methods in the leydig cells and in the acrosome region of maturing s ...	1987	3294830
purification and characterization of vibrio vulnificus protease.	a protease was purified from a strain of vibrio vulnificus isolated from the blood of a septicemic human. the vibrio was cultured in bacto peptone-yeast extract medium, and the protease was purified by a purification procedure including ultrafiltration of the culture supernatant with an amicon ym 5 membrane, diethylaminoethyl-sephacel column chromatography, sephacryl s-200 column chromatography and fast protein liquid chromatography on mono q column. the protease preparation revealed homogeneity ...	1987	3295490
effect of posttransplantation administration of peripheral blood lymphocytes in skin-grafted mice treated with antilymphocyte serum or antilymphocyte serum plus bone marrow.	posttransplantation administration of donor-specific bone marrow cells to als-treated allograft recipients produces graft survival that is greater than that produced by als treatment alone. we have now studied the effect of peripheral blood lymphocytes (pbls) in a mouse skin allograft model using this protocol (c3h skin grafted to b6af1 mice, day 0; i.p. als on days -1 and +2; i.v. bone marrow days +6 or +7). when pbls are injected in place of bone marrow cells, graft survival is extended beyond ...	1987	3299924
purification and characterization of an elastolytic protease of vibrio vulnificus.	large amounts of a highly purified, extracellular elastolytic protease of vibrio vulnificus were obtained by sequential ammonium sulphate precipitation and hydrophobic interaction chromatography with phenyl-sepharose cl-4b. the protease had an mr of about 50,500 (estimated by sds-page), a pi of 5.7, and a temperature optimum range of 55 to 60 degrees c. the ph optimum and the results of inactivation studies suggested that the enzyme was a neutral metalloprotease. the protease had about 429 amino ...	1987	3312481
vibrio vulnificus bacteremia: report of a case and review of the literature.		1987	3316128
effect of acute renal failure on insulin disposition in the isolated perfused rat kidney.	the renal disposition of insulin in acute renal failure has not been evaluated. we used the isolated perfused rat kidney to test the hypothesis that acute renal failure (arf) decreases renal insulin clearance. we used warm ischemia for 45 min, uranyl nitrate 5 mg/kg, ureter ligation, and nonfiltering kidneys as methods of inducing arf. comparisons were made with normal control kidneys. the concentrations of insulin in perfusate and urine was determined by radioimmunoassay. acute renal failure ca ...	1987	3318499
omental pedicle graft in the management of infected ascending aortic prostheses.	two patients had mediastinal infections with chronic draining sinus tracts that involved a vascular prosthesis in the ascending aorta. in 1 patient, a false mycotic aneurysm developed and in the other, a beginning rupture of the proximal suture line. in both patients, the infection was cured by replacing the infected aortic prosthesis combined with wrapping the new prosthesis with a pedicled omental graft. an omental graft was used to protect the vascular prosthesis and minimize the risk of recu ...	1987	3318738
t cell factors involved in the isotype-specific regulation of the ige antibody response.		1987	3333779
biosynthesis and processing of platelet gpiib-iiia in human megakaryocytes.	platelet membrane glycoprotein iib-iiia forms a calcium-dependent heterodimer and constitutes the fibrinogen receptor on stimulated platelets. gpiib is a two-chain protein containing disulfide-linked alpha and beta subunits. gpiiia is a single chain protein. these proteins are synthesized in the bone marrow by megakaryocytes, but the study of their synthesis has been hampered by the difficulty in obtaining enriched population of megakaryocytes in large numbers. to examine the biosynthesis and pr ...	1987	3108266
stimulation by hyphopichia burtonii and bacillus amyloliquefaciens of aflatoxin production by aspergillus flavus in irradiated maize and rice grains.	aspergillus flavus was grown on maize and rice extract agars and on irradiated viable cracked maize and rice grains, either in pure culture or in dual culture with wild strains of either hyphopichia burtonii or bacillus amyloliquefaciens. aflatoxin production by a. flavus and its growth and interactions with the other microorganisms were studied at three water activities (aw) (0.98, 0.95, and 0.90) and two temperatures (25 and 16 degrees c). both h. burtonii and b. amyloliquefaciens markedly sti ...	1987	3111368
comparison of glycosidase levels in bovine seminal plasma.	seven glycosidases (beta-n-acetylglucosaminidase, alpha-fucosidase, beta-galactosidase, acid alpha-glucosidase, beta-glucuronidase, acid and neutral alpha-mannosidase) were analysed in seminal plasma from the first and second successive ejaculates in normal ayrshire bulls. in comparison to our previous data the results indicate that beta-n-acetylglucosaminidase, beta-galactosidase and beta-glucuronidase are derived mainly from epididymal secretions, while alpha-fucosidase and particularly neutra ...	1987	3112030
inhibitory effect of capsular antigen of vibrio vulnificus on bactericidal activity of human serum.	opaque (op) and translucent (tr) colonial variants were isolated from vibrio vulnificus strains. op-type variants were more resistant than the isogenic tr-type variants, but the survival rate of the op-type variants varied with the strains. antisera were prepared by immunizing rabbit with whole cells of op and tr variants of some strains, in which the difference of the sensitivity between op and tr cells was remarkable. then agglutination tests with their living and heat-killed cells were carrie ...	1987	3116370
purification of an octamer sequence (atgcaaat)-binding protein from human b cells.	the highly conserved octamer sequence atgcaaat or its inverse complement found in all human and murine immunoglobulin gene promoters has been demonstrated to be necessary in the lymphoid-specific transcription by deletion analysis. trans-acting factors that interact with the octamer motif are thought to be involved in this tissue-specific expression. using a gel mobility shift assay, we have identified both lymphoid-specific and ubiquitous nuclear factors that interact with a human gamma 1 heavy ...	1987	3122182
[primary leiomyosarcoma of the pulmonary artery. apropos of a case].	a case of leiomyosarcoma of the pulmonary artery in a 64-year old man without previous cardiovascular disease is reported. the clinical picture, which comprised episodes of paroxysmal dyspnoea associated with acute cor pulmonale, suggested pulmonary embolism. radioisotope perfusion study and pulmonary angiography seemed to confirm this diagnosis, but no improvement was obtained with a prolonged thrombolytic treatment. the presence of a median mass at ct led to exploratory thoracotomy and to the ...	1987	3122693
covalent coupling of 4-thiouridine in the initiator methionine trna to specific lysine residues in escherichia coli methionyl-trna synthetase.	a new method has been developed to couple a lysine-reactive cross-linker to the 4-thiouridine residue at position 8 in the primary structure of the escherichia coli initiator methionine trna (trnafmet). incubation of the affinity-labeling trnafmet derivative with e. coli methionyl-trna synthetase (metrs) yielded a covalent complex of the protein and nucleic acid and resulted in loss of amino acid acceptor activity of the enzyme. a stoichiometric relationship (1:1) was observed between the amount ...	1987	3122828
differential effects of serotonin (5-ht1a and 5-ht2) agonists and antagonists on renin and corticosterone secretion.	the present study was designed to investigate the effect of distinct serotonin (5-ht1a and 5-ht2) agonists and antagonists on renin and corticosterone secretion. low doses of the selective 5-ht1a agonists 8-hydroxy-2-(di-n-propylamino)tetralin (8-oh-dpat) (5.0-500.0 micrograms/kg, i.p.) and ipsapirone (tvx q 7821; 0.5-2.5 mg/kg, i.p.), and of the 5-hydroxytryptamine (5-ht) agonist mk-212 (2.0 mg/kg, i.p.), did not elevate plasma renin activity (pra) and concentration (prc) 30 min postinjection. ...	1987	2952898
differential effects of serotonin (5-ht1a and 5-ht2) agonists and antagonists on renin and corticosterone secretion.	the present study was designed to investigate the effect of distinct serotonin (5-ht1a and 5-ht2) agonists and antagonists on renin and corticosterone secretion. low doses of the selective 5-ht1a agonists 8-hydroxy-2-(di-n-propylamino)tetralin (8-oh-dpat) (5.0-500.0 micrograms/kg, i.p.) and ipsapirone (tvx q 7821; 0.5-2.5 mg/kg, i.p.), and of the 5-hydroxytryptamine (5-ht) agonist mk-212 (2.0 mg/kg, i.p.), did not elevate plasma renin activity (pra) and concentration (prc) 30 min postinjection. ...	1987	2952898
experimental echinococcus granulosus infection in mice: immunocytochemical analysis of lymphocyte populations in local lymphoid infections during early infection.	the influence of subcutaneous infection with protoscoleces of echinococcus granulosus on the distribution of lymphocyte subsets in draining lymph nodes has been evaluated by immunocytochemical labelling of lymphocyte surface antigens. these studies reveal marked expansion of paracortical thy-1+, lyt-1+ cells and moderate proliferation of surface immunoglobulin-bearing b-cells immediately after infection. the lyt-1+:lyt-2+ ratio decreases rapidly during the first 21 days post-infection and remain ...	1987	2956563
[fatal septicemia of vibrio vulnificus in a patient with chronic hepatitis].		1987	2836645
nutrition in renal disease: the adult on dialysis.		1987	260630
[environment and quality of sleep in the hospital milieu].		1987	260315
mechanism of the epsp synthase catalyzed reaction: evidence for the lack of a covalent carboxyvinyl intermediate in catalysis.	in order to detect covalent reaction intermediates in the 5-enolpyruvylshikimate-3-phosphate (epsp) synthase reaction, we have investigated the interaction of epsp synthase with the reaction product epsp. an exchange of epsp-methylene protons could be demonstrated by incubating epsps with epsp in d2o. since trace amounts of contaminating pi would lead to reversal of epsps reaction and hence methylene proton exchange, we added pyruvate kinase, adp, mg++ and k+. under these conditions, any contami ...	1988	2838023
transcriptional regulation of the gene encoding the major surfactant protein (sp-a) in rabbit fetal lung.	the expression of the major protein of rabbit pulmonary surfactant (sp-a), a glycoprotein of mr congruent to 29,000-36,000, is regulated during development and by hormones. in the present study, utilizing a cdna insert complementary to mrna coding for sp-a and nuclear transcription elongation assays, we have investigated the developmental and hormonal regulation of transcription of the sp-a gene in rabbit fetal lung tissue. the relative rates of transcription of sp-a mrna increased as a function ...	1988	2848814
[the entero-insular axis: the new incretin candidate glucagon-like peptide-1(7-36)amide (glp-1(7-36))amide].	the functional connection between the gut and pancreatic islets is described by the term "enteroinsular axis". a humoral factor of the gut that might enhance the glucose-induced secretion of insulin is named "incretin". for many years glucose-dependent insulin-releasing polypeptide (gip) was the strongest incretin candidate. however, recent evidence suggests that glucagon-like peptide-1(7-36)amide represents a more potent physiological incretin. the sequence of glp-1 is identical in various mamm ...	1988	2849249
factors influencing the severity of escherichia coli and avian adenovirus group ii infections in chickens.	chickens from lines selectively bred for either a high (hh) or low (ll) antibody response to sheep erythrocytes were challenged intravenously with avian adenovirus group ii (aa). spleen size was determined 6 days later. in some experiments the responses of chickens to aa and escherichia coli infections were compared. the level of corticosterone in the feed (15 mg/kg) which resulted in the lowest incidence of pericarditis in response to e. coli resulted in the greatest incidence of large spleens ...	1988	2849405
suppression of sv40-promoted gene expression by differentiation of preadipose cells.	when a plasmid bearing the chloramphenicol acetyltransferase (cat) gene under the control of an sv40 early promoter is introduced into preadipose or adipose cells of line 3t3-f442a, the promoter directs high levels of transient expression of cat. however, when the plasmid is introduced into preadipose cells and the cells are then allowed to differentiate into adipose cells, the expression of the cat gene is suppressed. in this process, the plasmid is not changed detectably in amount, topology, o ...	1988	2849579
[changes in the cyclic nucleotide system of t-lymphocytes in experimental tuberculosis in animals].		1988	2851783
a murine monoclonal antibody raised against human non-small cell carcinoma of the lung.	murine monoclonal antibodies (mabs) reactive with human non-small cell carcinoma of the lung (nsccl) were produced following immunization with a membrane preparation of calu-3, a human adenocarcinoma of the lung cell line grown in nude mice. positive hybrids unreactive with normal liver membranes and human peripheral white blood cells were selected for further testing. one mab (rs5-4h6) recognized an antigen expressed in a variety of lung and other carcinoma cell lines as detected by flow cytome ...	1988	2853133
a multicentre open clinical trial of teicoplanin in infections caused by gram-positive bacteria.	a multicentre open trial of teicoplanin in 81 centres in nine european countries included 1431 cases: 531 female, 900 male; mean age 49.4 years, range 1-93 years. these were hospitalized patients most of whom had infections caused by staphylococcus aureus (816 isolates). of a total of 1427 gram-positive pathogens 280 (19.6%) were methicillin resistant. there were 536 skin and soft tissue infections, 263 septicaemias, 135 lower respiratory tract infections, 179 joint and bone infections and 83 en ...	1988	2965133
cns-induced natriuresis during dopamine receptor blockade. further support for the existence of, at least, two separate natriuretic hormonal systems.	intracerebroventricular (icv) stimulation with hypertonic sodium chloride solutions has previously shown indications to stimulate the release of a blood-borne natriuretic factor. sodium excretion in this situation increases in the order of 10-20 times. acute isotonic volume expansion has shown to be a potent stimulus for endogenous release of the atrial natriuretic factor (anf). it has also been demonstrated that the natriuretic response to both volume expansion and exogenously applied anf can b ...	1988	2976234
purification and partial characterization of serum monocytotropic factor, a platelet-derived cyclooxygenase-inducing polypeptide.	it has previously been shown that a heat- and acid-stable component of human and animal sera was capable of stimulating prostanoid biosynthesis in human blood monocytes, very probably by a mechanism involving cyclooxygenase induction. many physico-chemical characteristics of this factor are similar to those of identified platelet factors. here we show that human platelets are a rich source of this factor (serum monocytotropic factor) and that results from experiments using arachidonic acid or th ...	1988	3124880
decreased locomotor activity produced by repeated, but not single, administration of murine-recombinant interferon-gamma in mice.	a single treatment with murine-recombinant interferon-gamma (murifn-gamma; 30 micrograms/mouse), whether evaluated immediately after, or four hrs after, intraperitoneal injection, does not alter open field activity levels. on the other hand, repeated murifn-gamma administration (30 micrograms/mouse/day for 5 days) results in decreased spontaneous locomotor activity and an increase in body weight.	1988	3126371
adult epiglottitis due to vibrio vulnificus.		1988	3130933
hiv seroprevalence in migrant and seasonal farmworkers--north carolina, 1987.		1988	3136308
is vitellogenin an ancestor of apolipoprotein b-100 of human low-density lipoprotein and human lipoprotein lipase?	vitellogenin, an ancient animal protein, is the major yolk protein of eggs, where it is used as a food source during embryogenesis. here it is shown that vitellogenins, including those from the invertebrates caenorhabditis elegans and drosophila melanogaster, contain domains that are homologous with parts of apolipoprotein b-100 (apob-100) of human low-density lipoprotein and human lipoprotein lipase. as vitellogenins are likely to have been used by invertebrates during embryogenesis well before ...	1988	3145737
poor lifestyle modification among intravenous drug users assisted at a sexually transmitted disease clinic for hiv infection diagnosis, madrid, 1987.		1988	3146273
isolation of the early phase of chylomicron formation in intestinal epithelial cells of rats.	lipid is first observed electron microscopically in the rough endoplasmic reticulum of intestinal epithelial cells during active lipid absorption. we have been able to isolate this subcellular fraction by using discontinuous sucrose gradients of 0.25/0.86/1.11 m sucrose. a preliminary low speed centrifugation of mucosal homogenate removed the heavier subcellular organelles. the resulting supernatant was centrifuged at 5.25 x 10(6) x g.min. the pellet from this centrifugation was placed on top of ...	1988	3147719
structural and functional studies of the effects of sympathetic nerve stimulation on rabbit submandibular salivary glands.	continuous sympathetic stimulation at 8-10 hz caused intense vasoconstriction in the gland, so stimulation was generally given in an interrupted pattern to minimize this detrimental effect on secretion. only a small increase in fluid secretion occurred; it became thick and tended to block the cannula; therefore in later experiments the main duct was not cannulated. after sympathetic stimulation there was substantial degranulation of acinar cells. however, as this was accompanied by little moveme ...	1988	3165262
an interstitial deletion of chromosome 7 may characterize a subgroup of uterine leiomyoma.		1988	3165693
vibrio vulnificus necrotizing fasciitis presenting with compartmental syndrome of the hand.	vibrio vulnificus necrotizing fasciitis is an unusual clinical problem. when it presented as a compartment syndrome, the picture was obscured. we report a rare case of such a presentation. a below-elbow amputation was performed and the patient recovered.	1988	3171309
coagglutination of vibrio cholerae, vibrio mimicus, and vibrio vulnificus with anti-flagellar monoclonal antibody.	monoclonal antibodies (mabs) with serological activity for purified flagellar (h) core protein prepared from vibrio cholerae were identified by enzyme-linked immunosorbent assay. four of these mabs reacted with the flagella of v. cholerae and v. mimicus exclusively, while eight mabs reacted with at least 1 of 30 heterologous vibrio species tested by enzyme-linked immunosorbent assay or coagglutination. it appears that v. cholerae and v. mimicus express similar, if not identical, h determinants u ...	1988	3182988
septicaemia caused by vibrio vulnificus.	vibrio vulnificus is an uncommon cause of septicaemia. a few reports suggest that patients with chronic liver disease are more susceptible. we report two cases of septicaemia caused by this organism and emphasise the need for early empirical therapy.	1988	3183404
vibrio vulnificus meningitis in a boy with thalassemia after eating raw oysters.		1988	3186359
vibrio vulnificus and pulmonary infection.		1988	3195881
the effect of alcoholic beverages on iron and zinc metabolism in the rat.	1. male wistar rats (approximately 200 g) were given distilled water and a semi-synthetic control diet for 6 d. on day 7, 37 kbq 65zn were administered intramuscularly and the rats were given distilled water, beer, cider, red wine, whisky or ethanol as their only source of fluid. the wine, whisky and ethanol were diluted so that each of the beverages contained a similar ethanol concentration (approximately 30 g/l). food and fluid intake, growth rate and whole-body 65zn were measured regularly ov ...	1988	3196669
in vivo electrochemical detection of 5-hydroxyindole within the trigeminal nucleus caudalis of freely moving rats: the effect of morphine.	the trigeminal nucleus caudalis is considered the equivalent of the orofacial nociceptive system of the dorsal horn of the spinal cord. at the level of this trigeminal area (i.e. medullary dorsal horn) the transmission of noxious inputs is strongly modulated by a descending, serotonergic system mainly originating from the nucleus raphe magnus (nrm). the present study in freely moving animals reports the effect of morphine on the 5-hydroxyindole oxidation current recorded in the medullary dorsal ...	1988	3196919
vibrio vulnificus infections.		1988	3197443
differential toxicity of cis and trans isomers of dichlorodiammineplatinum.	nephrotoxicity is the dose-limiting toxic effect of cis-dichlorodiammineplatinum (cis-platin) in humans. its stereoisomer transplatin does not have any toxicity at equimolar concentrations, and it also possesses little antitumor activity. in this study, subcellular localization of both the platinum isomers was examined in the liver and kidney of the mouse 24 hours following the drug administration. levels of the platinum isomers were measured using flameless atomic absorption. the results showed ...	1988	3199415
exploitation of bedding in dairy outdoor calf hutches by immature house and stable flies (diptera: muscidae).		1988	3204628
protomyobia (acari: myobiidae) species groups and the brevisetosa complex of mites on north american shrews (mammalia: soricidae).		1988	3204630
[clinical studies of rokitamycin dry syrup on skin and soft tissue infections in the pediatric fields].	rokitamycin (rkm) dry syrup, a newly developed macrolide antibiotic, was administered to children with ages between 6 months and 15 years and 10 months suffering from skin and soft tissue infections including 41 cases of impetigo, one case of staphylococcal scalded skin syndrome (ssss) and 2 cases of subcutaneous abscess totalling 44 cases. the average daily dose level used was 31.3 mg/kg divided into 3 or 4 doses, for an average of 6 days of treatment. mics of 4 different macrolide antibiotics ...	1988	3204657
acute hepatic failure precipitated in a patient with subclinical liver disease by vibrionic and clostridial septicemia.	a case of fatal septicemia caused by vibrio vulnificus and clostridium bifermentans in a patient with subclinical liver disease is described. the patient appeared to recover from the infection initially after antibiotic therapy but finally succumbed to rapidly developing hepatic failure. postmortem examination revealed hemochromatosis. the pathogenesis of the polymicrobial septicemia and hepatic failure is discussed in the light of the published literature.	1988	3211597
in vitro activity of sulbactam/ampicillin and ampicillin against methicillin-sensitive and methicillin-resistant staphylococcus aureus and staphylococcus epidermidis.	minimum inhibitory concentrations (mics) of ampicillin and ampicillin + sulbactam (1:1) against 165 strains of staphylococcus aureus and 72 strains of staphylococcus epidermidis have been evaluated. the activity of the combination was very good. a concentration of 16 micrograms/ml + 16 micrograms/ml inhibited 96.9% of s. aureus and the 100% of s. epidermidis strains (at the same concentration ampicillin alone inhibited only 55.15% and 56.9% of s. aureus and s. epidermidis strains respectively). ...	1988	3224398
in vitro effect of xipamide on sodium-potassium transport systems in human erythrocytes.	the in vitro effects of xipamide in a concentration range of 10(-8) to 10(-2) m were investigated on various na+ and k+ transport systems in human red blood cells. xipamide inhibited the anion carrier or dids-sensitive lico3- -influx starting from a concentration of 10(-5) m. however, a decrease in the na+, k+-pump and the na+, k+-cotransport activity and a rise in the passive permeability of the cell membrane was only observed starting from a concentration of 10(-4) m xipamide.	1988	3226221
excision of cutaneous tumors in the horse using histologic guidance.	a highly successful surgical technique for removing cutaneous tumors in humans was used in seven horses with cutaneous squamous cell carcinoma (n = 3) or sarcoid (n = 4). in humans, the complete underside of the tumor is evaluated histologically by processing horizontal sections from the tumor base, and orientation between the wound surface and the undersurface of the excised tumor is maintained by mapping both surfaces. the technique ensures that small foci of residual tumor can be located accu ...	1988	3227634
cytolytic toxins of vibrio vulnificus and vibrio damsela.		1988	3231100
[epidemiology and developmental aspects of trypanosomiasis in the source of the sangha, congo].		1988	3233035