Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| the na+/e- stoichiometry of the na+-motive nadh:quinone oxidoreductase in vibrio alginolyticus. | a method is proposed to estimate the stoichiometries of primary na+-pumps in intact bacterial cells. it is based on technique when the h+/e- stoichiometry is measured in the presence of protophorous uncoupler and in the absence of penetrating ions other than h+. under these conditions, the h+ influx discharges membrane potential generated by the na+ pump so the na+/e- and h+/e- ratios become equal. using this approach it is shown that the na+/e- ratio for the na+-motive nadh:quinone oxidoreducta ... | 1997 | 9224712 |
| putative channel components for the fast-rotating sodium-driven flagellar motor of a marine bacterium. | the polar flagellum of vibrio alginolyticus rotates remarkably fast (up to 1,700 revolutions per second) by using a motor driven by sodium ions. two genes, motx and moty, for the sodium-driven flagellar motor have been identified in marine bacteria, vibrio parahaemolyticus and v. alginolyticus. they have no similarity to the genes for proton-driven motors, mota and motb, whose products constitute a proton channel. motx was proposed to be a component of a sodium channel. here we identified additi ... | 1997 | 9260952 |
| effects of extracellular products of vibrio alginolyticus on penaeid prawn plasma components. | the effects of both crude extracellular products (ecp) and a partially purified protease of vibrio alginolyticus on the plasma components of kuruma prawn (penaeus japonicus) and tiger prawn (p. monodon) were studied using crossed immunoelectrophoresis (cie). a component of the plasma, tentatively identified as coagulogen, apparently disappeared after incubation with the ecp, while the amount of a component tentatively identified as haemocyanin decreased. the coagulogen and an unknown component ( ... | 1997 | 9281857 |
| h+-pumping atpase has little stimulatory effect on in vitro translocation of a model protein into vibrio alginolyticus inside-out membrane vesicles. | we studied the effect of a h+ electrochemical potential generated by f1f0 atpase on in vitro translocation of a model protein into vibrio alginolyticus inside-out membrane vesicles. the f1f0 atpase of v. alginolyticus catalyzed the pumping of h+ coupled to atp hydrolysis as measured in fluorescence quenching experiments. consequently, this enzyme leads to the generation of a h+ electrochemical potential. the h+ electrochemical potential generated by f1f0 atpase was completely abolished by 30 mic ... | 1997 | 9339889 |
| cloning of a vibrio alginolyticus rpon gene that is required for polar flagellar formation. | a fragment of dna was cloned which complemented a polar flagellum-defective (pof) mutation of vibrio alginolyticus. the fragment contained two complete and two partial open reading frames (orfs) (orf2 and -3 and orf1 and -4, respectively). the presumed product of orf2 has an amino acid sequence with a high degree of similarity to that of rpon, which is an alternative sigma factor (sigma54) for other microorganisms. the other orfs are also homologous to the genes adjacent to other rpon genes. del ... | 1997 | 9352939 |
| generation of reactive oxygen species by raphidophycean phytoplankton. | chattonella marina, a raphidophycean flagellate, is one of the most toxic red tide phytoplankton and causes severe damage to fish farming. recent studies demonstrated that chattonella sp. generates superoxide (o2-), hydrogen peroxide (h2o2), and hydroxyl radicals (.oh), which may be responsible for the toxicity of c. marina. in this study, we found the other raphidophycean flagellates such as heterosigma akashiwo, olisthodiscus luteus, and fibrocapsa japonica also produce o2- and h2o2 under norm ... | 1997 | 9362113 |
| the na+-translocating nadh:ubiquinone oxidoreductase from vibrio alginolyticus--redox states of the fad prosthetic group and mechanism of ag+ inhibition. | the fad prosthetic group of the na+-motive nadh:ubiquinone oxidoreductase (na+-nqr) from vibrio alginolyticus was investigated by ultraviolet-visible and fluorescence spectroscopy. the reduction of na+-nqr by excess nadh in the presence of 6-13 microm o2 resulted in the formation of the blue flavosemiquinone radical. if the concentration of dioxygen was further reduced to 0.1 microm o2, neither the reduction of na+-nqr by nadh nor its reoxidation with ubiquinone-1 (q-1) yielded a stable flavosem ... | 1997 | 9395325 |
| evaluation of vitek gni+ and becton dickinson microbiology systems crystal e/nf identification systems for identification of members of the family enterobacteriaceae and other gram-negative, glucose-fermenting and non-glucose-fermenting bacilli. | we evaluated the vitek gni+ and becton dickinson crystal e/nf identification systems for their ability to accurately identify 619 and 626 strains, respectively, of members of the family enterobacteriaceae and other glucose-fermenting and non-glucose-fermenting gram-negative rods. all strains tested were taken from a stock collection and passed three times on 5% sheep blood agar prior to testing. these strains represented a more rigorous challenge to both systems than one resulting from the testi ... | 1997 | 9399532 |
| [bacterial diversity during the cholera epidemic in dakar, senegal (1995-1996)]. | vibrio cholerae o:1, serotype ogawa and biotype el tor (76.1%) was responsible of the outbreak of cholera in dakar, senegal (1995-1996). however, other bacteria were isolated, particularly vibrio cholerae non o:1/non o:139, vibrio fluvialis, vibrio alginolyticus. vibrio parahaemolyticus, salmonella sp.p, shigella sp.p (23.9%). the vibrio cholerae o:1 strains are multiresistant to sulfonamide, cotrimoxazole and chloramphenicol. 97% were also resistant to o/129 compound. fluoroquinolone and 3rd ge ... | 1997 | 9410248 |
| polarizing response of human polymorphonuclear cells to vibrio cholerae. | leucocytes have the capacity to respond to chemotactic factors by becoming morphologically and functionally polarized and this method has been found to be suitable for measurement of chemotaxis. this work evaluates the effect of whole vibrio cholerae biotype el tor polarization of human polymorphonuclear (pmn) cells in comparison with strains of v. cholerae nag, vibrio alginolyticus and salmonella typhi. v. cholerae o1 induced, at different cell/bacteria ratios, a significant increase in the per ... | 1997 | 9503599 |
| the fatty acid composition of a vibrio alginolyticus associated with the alga cladophora coelothrix. identification of the novel 9-methyl-10-hexadecenoic acid. | the fatty acid composition of a new strain of vibrio alginolyticus, found in the alga cladophora coelothrix, was studied. among 38 different fatty acids, a new fatty acid, 9-methyl-10-hexadecenoic acid and the unusual 11-methyl-12-octadecenoic acid, were identified. linear alkylbenzene fatty acids, such as 10-phenyldecanoic acid, 12-phenyldodecanoic acid and 14-phenyltetradecanoic acid, were also found in v. alginolyticus. the alga contained 43% saturated fatty acids, and 28% c16-c20 polyunsatur ... | 1997 | 9438237 |
| structure and function of the genes involved in mannitol, arabitol and glucitol utilization from pseudomonas fluorescens dsm50106. | a dna fragment from pseudomonas fluorescens dsm50106 containing the genes for the uptake and utilization of mannitol, arabitol and glucitol was cloned in escherichia coli and sequenced. seven open reading frames (mtlefgkdyz) were identified on the 10031 bp fragment. the deduced amino acid sequences of the first four open reading frames (mtlefgk) revealed significant similarity to the components of the maltose transport system in e. coli and salmonella typhimurium. the gene mtld encoding a polyol ... | 1998 | 9461423 |
| cloning and nucleotide sequence of the gyrb gene of vibrio parahaemolyticus and its application in detection of this pathogen in shrimp. | because biochemical testing and 16s rrna sequence analysis have proven inadequate for the differentiation of vibrio parahaemolyticus from closely related species, we employed the gyrase b gene (gyrb) as a molecular diagnostic probe. the gyrb genes of v. parahaemolyticus and closely related vibrio alginolyticus were cloned and sequenced. oligonucleotide pcr primers were designed for the amplification of a 285-bp fragment from within gyrb specific for v. parahaemolyticus. these primers recognized ... | 1998 | 9464408 |
| attachment of vibrio alginolyticus to glass surfaces is dependent on swimming speed. | the attachment of vibrio alginolyticus to glass surfaces was investigated with special reference to the swimming speed due to the polar flagellum. this bacterium has two types of flagella, i.e., one polar flagellum and numerous lateral flagella. the mutant ym4, which possesses only the polar flagellum, showed much faster attachment than the mutant ym18, which does not possess flagella, indicating that the polar flagellum plays an important role. the attachment of ym4 was dependent on na+ concent ... | 1998 | 9473049 |
| identification of six subunits constituting na+-translocating nadh-quinone reductase from the marine vibrio alginolyticus. | we previously reported that the purified na+-translocating nadh-quinone reductase (nqr) from the marine vibrio alginolyticus is composed of three major subunits, alpha, beta and gamma. nqr operon was sequenced and was found to be composed of 6 structural genes. among these genes, nqr1, nqr3 and nqr6 were identified to code for alpha-, gamma- and beta-subunits, respectively. the protein products from nqr2, nqr4 and nqr5, however, were not reported. the sequence data predicted that these three pro ... | 1998 | 9490015 |
| overexpression in escherichia coli of the rnf genes from rhodobacter capsulatus--characterization of two membrane-bound iron-sulfur proteins. | the rnf genes of rhodobacter capsulatus, essential for nitrogen fixation, are thought to encode a system for electron transport to nitrogenase. in the present study, we have attempted to overexpress the rnf genes in escherichia coli to investigate the molecular properties of the corresponding proteins. corrections were made to the published dna sequence of the rnf operon, resulting in the identification of two genes, rnfg and rnfh. the rnfabcdgeh operon thus comprises seven genes and shows simil ... | 1998 | 9492268 |
| ct metrizamide myelography for intraspinal and paraspinal neoplasms in infants and children. | computed tomography (ct) metrizamide myelography is an accurate and satisfactory method for examining the spinal cord in infants and children with suspected intraspinal neoplasms. it was performed in 13 infants and children and in one adult with intra- and paraspinal neoplasms. ct metrizamide myelography in paraspinal masses provides the total geography of the mass; that is, possible intraspinal extension, as well as the paraspinal portion itself. as such, it is superior to conventional myelogra ... | 1998 | 106638 |
| immunocytochemical characterization of the lymphocytes in nodular lymphoid hyperplasia of the bowel. | the immunoglobulin constituents of hypertrophied lymphoid nodules in the intestinal tracts of six patients with the variable immunodeficiency syndrome and one patient with selective iga deficiency were evaluated by the peroxidase-labeled antibody technique. the nodules were found to contain a dense population of igm-bearing lymphocytes and much intercellular igm. evidence that the cells were engaged in igm synthesis was the presence of the immunoglobulin in the perinuclear spaces and endoplasmic ... | 1998 | 105208 |
| catheter cervical vertebral venography. | cervical vertebral venography using catheterzation via the femoral vein was carried out in 78 cases. in disc disease the internal vertebral venous plexus (ivvp) are interrupted at single or multiple levels, and sometimes completely blocked. in ossification of posterior longitudinal ligament, the ivvp are separated bilaterally and interrupted at multiple levels due to centrally situated ossification and associated osteophytes. venography helps to determine the upper and lower limits of laminectom ... | 1998 | 106325 |
| high-performance liquid chromatographic analysis of penicillin v benzathine oral suspensions. | a rapid high-performance liquid chromatographic assay for penicillin v content of penicillin v benzathine bulk drug and oral suspensions is described. dilution of the oral suspension with methanol containing 1,3,5-trimethoxybenzene as the internal standard allowed for direct analysis on a reversed-phase column with a mobile phase of 53% methanol in 0.05 m aqueous ph 3.5 phosphate buffer. a relative standard deviation of less than 1% was obtained on commercial formulations, and the system was lin ... | 1998 | 107312 |
| congenital hypothyroidism control programs. a cost-benefit analysis. | comprehensive screening programs to control congenital hypothyroidism (ch), a preventable form of mental retardation, are being considered by some public health agencies. the proposed programs would test neonates' blood for thyroxine and, if warranted, provide follow-up testing and therapy. the estimated cost of detecting a single case of ch is $9,300, which includes specimen collection, laboratory analysis, and retesting of border-line cases. the present value of the treatment costs of ch adds ... | 1998 | 108409 |
| the oral hypoglycemic controversy. stay as sweet as you are. | 1998 | 106193 | |
| opposite effects of one and three injections of cortisone or thyroxine on intestinal lactase activity in suckling mice. | a single injection of cortisone or thyroxine to 8-day-old suckling mice initiates a temporary decrease of lactase activity. on the contrary, 3 injections of cortisone or thyroxine provoke a significant increase of lactase activity. it appears that the mechanism which controls the postnatal development of lactase in suckling animals is more complex than expected. | 1998 | 108133 |
| radiographic changes of the skull in sickle cell anemia. | skull radiographs of 194 patients from 4 months to 55 years old with sickle cell anemia revealed porous decreased bone density in 25%, widening of diploë associated with a relative decrease in the width of the outer table in 22%, and vertical "hair-on-end" striations in 5%. the youngest patient with vertical striations was 5 years old and the oldest was 39. serial examinations in 60 patients revealed no decrease of the skull width nor disappearance of the striations with age. | 1998 | 106639 |
| [acromegaly. recent physiopathological and therapeutic acquisitions]. | 1998 | 108635 | |
| rapid and accurate diagnosis of acute cholecystitis with 99mtc-hida cholescintigraphy. | technetium-99m dimethyl acetanilide iminodiacetic acid (hida) cholescintigraphy was performed on 90 patients with suspected acute cholecystitis. visualization of the gallbladder established patency of the cystic duct and excluded the diagnosis of acute cholecystitis in 50 of 52 patients. nonvisualization of the gallbladder with visualization of the common bile duct was diagnostic of acute cholecystitis in 38 patients, all subsequently proven at surgery. the observed accuracy of this procedure is ... | 1998 | 106679 |
| fetal heart rate during a maternal grand mal epileptic seizure. | although maternal ingestion of antiepileptic drugs is strongly suspected of causing congenital defects, particularly oral clefts, the effect of epilepsy itself or a combined effect of drug intake and epilepsy have not been excluded as etiological factors. very little is known about fetal oxygenation during a maternal grand mal epileptic seizure. we describe two cases in which fetal heart rate was recorded during a maternal epileptic seizure during labor. the first fetus became clearly asphyctic ... | 1998 | 106102 |
| topological study of vibrio alginolyticus nhab na+/h+ antiporter using gene fusions in escherichia coli cells. | nhab, an na+/h+ antiporter, of vibrio alginolyticus is a 528-amino-acid protein. hydropathy profile-based computer analysis predicted that the nhab might contain up to 13 membrane-spanning domains. to examine this hypothesis, we applied the phoa fusion method to the cloned nhab gene. eighteen plasmid-borne nhab-phoa fusion genes were constructed in escherichia coli cells and the alkaline phosphatase activity and expression level of the fusion proteins analyzed. these results and the results obta ... | 1998 | 9518558 |
| experimental vibriosis induction with vibrio alginolyticus of larvae of the catarina scallop (argopecten ventricosus =circularis) (sowerby ii, 1842) | 1998 | 9541555 | |
| chemotaxis of pathogenic vibrio strains towards mucus surfaces of gilt-head sea bream (sparus aurata l.). | vibrio anguillarum and vibrio alginolyticus exhibited significant adhesion to and chemotactic abilities towards mucus collected from the skin, gills, and intestine of gilt-head sea bream. quadratic polynomial models for chemotaxis designed to estimate the influence of temperature demonstrated a differential bacterial chemotaxis depending of the source of the mucus, with the chemotaxis towards intestinal mucus being the least influenced. | 1998 | 9575135 |
| flagellin-containing membrane vesicles excreted from vibrio alginolyticus mutants lacking a polar-flagellar filament. | polar flagellum-defective mutants (pof- laf-) have been isolated from a lateral flagella-defective mutant (pof+ laf-). among these pof- laf- mutants, polar-filamentless mutants, which have the hook structure but not the filament, were identified by electron microscopy. their hooks were covered with a sheath structure which is contiguous to the outer membrane. the filament proteins, flagellins, were shed into the culture medium of these mutants. these flagellins could be sedimented by high-speed ... | 1998 | 9604007 |
| ktrab, a new type of bacterial k(+)-uptake system from vibrio alginolyticus. | vibrio alginolyticus contained two adjacent genes, ktra and ktrb, which encode a new type of bacterial k(+)-uptake system. ktra and ktrb are peripheral and integral membrane proteins, respectively. six of the nine sequenced bacterial genomes contain homologs to both ktra and ktrb, suggesting that ktrab is widespread. | 1998 | 9642210 |
| cloning of the trkah gene cluster and characterization of the trk k(+)-uptake system of vibrio alginolyticus. | k(+)-uptake genes of vibrio alginolyticus were identified by cloning chromosomal dna fragments of this organism into plasmids, followed by electroporation and selection for growth at low k+ concentrations of cells of an escherichia coli strain defective in k+ uptake. a 4.1 kb dna fragment contained a cluster of three orfs on the same dna strand: the previously identified trka gene, a gene similar to e. coli trkh (v. alginolyticus trkh) and a new gene, orf1, whose function is not clear. products ... | 1998 | 9720051 |
| cloning and expression of the seca gene of a marine bacterium, vibrio alginolyticus, and analysis of its function in escherichia coli. | we report the cloning, sequencing and functional characterization of the seca gene of a marine bacterium, vibrio alginolyticus, which has been suggested to utilize atp and the sodium motive force for protein translocation. oligodeoxynucleotides corresponding to highly conserved regions of escherichia coli seca located in the high affinity atp binding site were utilized as pcr primers to clone the seca gene of v. alginolyticus. it was shown to encode a 103.3-kda protein. the deduced amino acid se ... | 1998 | 9729436 |
| necrotizing fasciitis due to vibrio alginolyticus following an injury inflicted by a stingray. | we describe the case of a patient with necrotizing fasciitis due to vibrio alginolyticus in a patient with cirrhosis following an injury inflicted by a stingray. the patient was successfully treated with aggressive surgical debridement and a combination of ciprofloxacin and amoxicillin-clavulanate. cases of invasive v. alginolyticus reported in the literature were reviewed. | 1998 | 9730311 |
| investigation of the 1994-5 ukrainian vibrio cholerae epidemic using molecular methods. | thirty-seven vibrio cholerae and four non-cholera vibrio isolates from ukraine, including strains from the epidemic of 1994-5, were analysed by molecular methods. results from pfge and ribotyping indicated that all ukrainian toxigenic v. cholerae were closely related to each other and to an isolate from a patient from pakistan. a non-toxigenic river water strain obtained during the height of the epidemic was more distantly related to these v. cholerae strains, while the vibrio parahaemolyticus i ... | 1998 | 9747752 |
| molecular cloning and functional characterization of sece of a marine bacterium, vibrio alginolyticus. | sece is an essential membrane component of the escherichia coli protein translocation machinery, which utilizes atp and the proton motive force as energy sources. the sece homologue of marine vibrio alginolyticus, in which protein translocation requires atp and the sodium motive force, was cloned, sequenced, and characterized. unlike most sece homologues found in various organisms, sece of v. alginolyticus (va-sece) possesses three transmembrane sequences like e. coli sece (ec-sece) and compleme ... | 1998 | 9791006 |
| pathogenicity of vibrio alginolyticus for cultured gilt-head sea bream (sparus aurata l.). | the in vivo and in vitro pathogenic activities of whole cells and extracellular products of vibrio alginolyticus for cultured gilt-head sea bream were evaluated. the 50% lethal doses ranged from 5.4 x 10(4) to 1.0 x 10(6) cfu/g of body weight. the strains examined had the ability to adhere to skin, gill, and intestinal mucus of sea bream and to cultured cells of a chinook salmon embryo cell line. in addition, the in vitro ability of v. alginolyticus to adhere to mucus and skin cells of sea bream ... | 1998 | 9797276 |
| survival of vibrio cholerae 01 strains in shrimp subjected to freezing and boiling. | this research was undertaken to assess the resistance of vibrio cholerae 01 strains inoculated into white shrimp, penaeus schimitti, to heating and freezing treatments. shrimp samples with and without carapace were obtained from sao luis, brazil. microbial analysis revealed the presence of marine vibrios including vibrio alginolyticus, vibrio parahaemolyticus, and other vibrios and aerobic gram-negative and gram-positive bacteria that grew on selective medium, thiosulfate-citrate-bile salt-sucro ... | 1998 | 9798148 |
| neurotoxic effect on two fish species and a pc12 cell line of the supernate of vibrio alginolyticus and vibrio anguillarum. | the biological effects of supernates obtained from different strains of vibrio alginolyticus and vibrio anguillarum isolated from diseased fish have been studied by inoculation on two fish species, eel and rainbow trout, and two fish cell lines. these supernates possess neuroexcitatory properties, and so, when they are injected into both fish species, they trigger convulsions, wriggling, contortive swimming and respiratory arrest coupled with increased respiratory reflex. furthermore, after the ... | 1998 | 9810622 |
| prevention of marine biofouling using a conductive paint electrode | conductive paint electrode was used for marine biofouling on fishing nets by electrochemical disinfection. when a potential of 1.2 v vs. a saturated calomel electrode (sce) was applied to the conductive paint electrode, vibrio alginolyticus cells attached on the electrode were completely killed. by applying a negative potential, the attached cells were removed from the surface of the electrode. changes in ph and chlorine concentration were not observed at potentials in the range -0.6 approximate ... | 1998 | 10099349 |
| tcpp protein is a positive regulator of virulence gene expression in vibrio cholerae. | the production of several virulence factors in vibrio cholerae o1, including cholera toxin and the pilus colonization factor tcp (toxin-coregulated pilus), is strongly influenced by environmental conditions. to specifically identify membrane proteins involved in these signal transduction events, we examined a transposon library of v. cholerae generated by tnbla mutagenesis for cells that produce tcp when grown under various nonpermissive conditions. to select for tcp-producing cells we used the ... | 1998 | 9435261 |
| retrospect and prospect of electron microscopy in japan: biology/virology. | 1998 | 2809471 | |
| [the cranio-carpo-tarsal dysplasia syndrome in a 6-year-old girl (whistling-face syndrome)]. | 1998 | 106974 | |
| staging of myeloma. a preliminary study of staging factors and treatment in different stages. | attempts were made to find prognostic factors in myeloma. in 16 deceased patients, urinary light chains, skeletal lesions, and the quantity of the monoclonal protein fraction in the serum were correlated to prognosis, in contrast to the electrophoretic mobility of the monoclonal fraction, the hemoglobin, the serum creatinine value, the serum calcium, or the intestinal calcium absorption. skeletal calcium uptake was only numerically higher in mild myeloma than in advanced myeloma. since these fin ... | 1998 | 106448 |
| neurofibromatosis: unusual lymphangiographic findings. | 1998 | 106665 | |
| the role of magnesium in protein-energy malnutrition. | the present study was aiming to explore further the variations in the levels of mg in both serum and csf of pem children, hoping a better understanding revealed decreased values of both blood serum and csf levels of mg in pem children which denote some abnormalities in brain metabolism. such abnormalities add further support for assumption of the link between dietary insufficiency and subnormal mental performance in pem. | 1998 | 98923 |
| radiation in multidisciplinary treatment of children with malignant ovarian tumors. | thirteen girls with malignant ovarian tumors treated at memorial hospital from august 1971 through october 1975 with extensive surgery, multi-drug chemotherapy (t-2 protocol) and postoperative radiation were reviewed. the plan of radiation was to deliver 3,000 rads to the whole abdomen and boost the para-aortic and pelvic area with 1,500 rads in five to six weeks. in only two patients was the radiation conducted as planned. in others the treatment was either interrupted or discontinued due to co ... | 1998 | 106952 |
| [pancreatic carcinoma on endoscopic retrograde pancreatico-cholangiogram (author's transl)]. | the pancreaticographic appearances of carcinoma of the pancreas have been divided into two types: 1. canalicular carcinoma arising from the duct system. if arising from the main pancreatic duct, it occludes this, or causes displacement or deformity of its branches in the immediate neighbourhood. if arising from branches, it causes cystic ectasia of the minor ducts; these appear fragmented and deformed while there is stenosis and displacement of the main duct. simultaneous origin from the main an ... | 1998 | 124684 |
| retinol-binding protein in malnutrition. | 1999 | 90984 | |
| dioda: delineation and feature extraction of microscopical objects. | a computer program is described for delineation and measurement of microscopical objects, such as cells and chromosomes, which may have been scanned using absorbance, fluorescence or reflectance microscopy. the quality of the object delineation is optimized through the controur ratio, which is simply computed from the object contour. geometrical features, like the perimeter and area are computed. a new definition is introduced for the background region, especially suited for the analysis of clos ... | 1999 | 93532 |
| hexacarbon neuropathy. | 1999 | 91030 | |
| on the reaction of iron bleomycin with thiols and oxygen. | 1999 | 91373 | |
| herniation of an intervertebral disc with cauda compression syndrome in childhood. | a case of a lumbosacral disc herniation of traumatic origin with compression of the cauda equina in a child is reported. the problems of early diagnosis, timely operative procedure, and prognosis of neurological deficit of operated disc herniation in childhood are discussed. | 1999 | 91675 |
| two effects of phosphodiesterase inhibitors on limulus ventral photoreceptors. | 1999 | 91407 | |
| the 𝛿-aminolevulinate dehydratase of marine vibrio alginolyticus is resistant to lead (pb). | 1999 | 28281822 | |
| suppression by the dna fragment of the motx promoter region on long flagellar mutants of vibrio alginolyticus. | the axial length of the polar flagellum (pof) of vibrio alginolyticus is about 5 microm. we previously isolated mutants that make abnormally long flagella. the swarm sizes of these mutants in a soft agar plate are smaller than that of a wild-type strain. we cloned a dna fragment into the pmf209 plasmid that restored the swarming ability of the long-pof strain v10578. the swimming speed and flagellar length of these transformants were almost equal to the wild-type values. the amounts of pf47 flag ... | 1999 | 10100745 |
| camp-mediated catabolite repression and electrochemical potential-dependent production of an extracellular amylase in vibrio alginolyticus. | vibrio alginolyticus, a halophilic marine bacterium, produced an extracellular amylase with a molecular mass of approximately 56,000, and the amylase appeared to be subject to catabolite repression mediated by camp. the production of amylase at ph 6.5, at which the respiratory chain-linked h+ pump functions, was inhibited about 75% at 24 hours following the addition of 2 microm carbonyl cyanide m-chlorophenylhydrazone (cccp), while the production at ph 8.5, at which the respiratory chain-linked ... | 1999 | 10192907 |
| the presumed potassium carrier trk2p in saccharomyces cerevisiae determines an h+-dependent, k+-independent current. | ionic currents related to the major potassium uptake systems in saccharomyces cerevisiae were examined by whole cell patch-clamping, under k+ replete conditions. those currents have the following properties. they (1) are inward under all conditions investigated, (2) arise instantaneously with appropriate voltage steps, (3) depend solely upon the moderate affinity transporter trk2p, not upon the high affinity transporter trk1p. they (4) appear to be independent of the extracellular k+ concentrati ... | 1999 | 10218594 |
| differentiation of vibrio alginolyticus strains isolated from sardinian waters by ribotyping and a new rapid pcr fingerprinting method. | we investigated the usefulness of a novel pcr fingerprinting technique, based on the specific amplification of genomic regions, to differentiate 30 vibrio alginolyticus strains isolated in sardinian waters. the different profiles obtained were scanned and analyzed by a computer program in order to determine genetic relationships. the results were then compared with the patterns obtained by ribotyping with hindiii, kpni, and xbai restriction enzymes. pcr fingerprinting could differentiate the str ... | 1999 | 10223972 |
| korormicin, an antibiotic specific for gram-negative marine bacteria, strongly inhibits the respiratory chain-linked na+-translocating nadh: quinone reductase from the marine vibrio alginolyticus. | 1999 | 10344574 | |
| vibrios associated with litopenaeus vannamei larvae, postlarvae, broodstock, and hatchery probionts. | several bacteriological surveys were performed from 1994 to 1996 at different litopenaeus vannamei hatcheries (in ecuador) and shrimp farms (in mexico). samples were taken from routine productions of healthy and diseased l. vannamei larvae, postlarvae, and their culture environment and from healthy and diseased juveniles and broodstock. in ecuador, the dominant bacterial flora associated with shrimp larvae showing symptoms of zoea 2 syndrome, mysis mold syndrome, and bolitas syndrome has been de ... | 1999 | 10347048 |
| occurrence, diversity, and pathogenicity of halophilic vibrio spp. and non-o1 vibrio cholerae from estuarine waters along the italian adriatic coast. | the occurrence, diversity, and pathogenicity of vibrio spp. were investigated in two estuaries along the italian adriatic coast. vibrio alginolyticus was the predominant species, followed by vibrio parahaemolyticus, non-o1 vibrio cholerae, and vibrio vulnificus. by using a biochemical fingerprinting method, all isolates were grouped into nine phenotypes with similarity levels of 75 to 97.5%. the production of toxins capable of causing cytoskeleton-dependent changes was detected in a large number ... | 1999 | 10347072 |
| comparison of the heme iron utilization systems of pathogenic vibrios. | vibrio alginolyticus, vibrio fluvialis, and vibrio parahaemolyticus utilized heme and hemoglobin as iron sources and contained chromosomal dna similar to several vibrio cholerae heme iron utilization genes. a v. parahaemolyticus gene that performed the function of v. cholerae huta was isolated. a portion of the tonb1 locus of v. parahaemolyticus was sequenced and found to encode proteins similar in amino acid sequence to v. cholerae hutw, tonb1, and exbb1. a recombinant plasmid containing the v. ... | 1999 | 10348876 |
| change to alanine of one out of four selectivity filter glycines in ktrb causes a two orders of magnitude decrease in the affinities for both k+ and na+ of the na+ dependent k+ uptake system ktrab from vibrio alginolyticus. | ktrab from vibrio alginolyticus is a recently described new type of high affinity bacterial k+ uptake system. its activity assayed in an escherichia coli k+ uptake negative mutant depended on na+ ions (km of 40 microm). subunit ktrb contains four putative p-loops. the selectivity filter from each p-loop contains a conserved glycine residue. residue gly-290 from the third p-loop selectivity filter in ktrb was exchanged for ala, ser or asp. ktrb variants ser-290 and asp-290 were without activity. ... | 1999 | 10359077 |
| cloning, sequencing, expression and characterization of the manganese superoxide dismutase gene from vibrio alginolyticus. | the soda gene coding for manganese superoxide dismutase from the marine microorganism vibrio alginolyticus was cloned, sequenced and over-expressed in escherichia coli using the pet20b (+) expression vector. the full-length gene was consisted of 603bp open reading frame, which encoded a polypeptide of 201 amino acid residues, with a calculated molecular weight of 22672da. the deduced amino acid sequence of the soda showed considerable homology to other mn-sods. the recombinant enzyme was efficie ... | 1999 | 10365251 |
| an evaluation of chromogenic substrates for characterization of serine protease produced by pathogenic vibrio alginolyticus. | four chromogenic substrates for characterizing serine protease of vibrio alginolyticus were evaluated. the protease activity of bacterial extracellular products, or the fractions of 33 kd protease purified by the akta purifier system with various columns, was completely inhibited by ethylenediamine tetra-acetic acid, ethylene glycol-bis(beta-amino-ethyl ether) n,n,n',n'-tetraacetic acid (egta), antipain and phenylmethylsulphonyl fluoride (pmsf) using water-soluble substrates (azoalbumin and azoc ... | 1999 | 10413876 |
| functional interaction between poma and pomb, the na(+)-driven flagellar motor components of vibrio alginolyticus. | four proteins, poma, pomb, motx, and moty, appear to be involved in force generation of the sodium-driven polar flagella of vibrio alginolyticus. among these, poma and pomb seem to be associated and to form a sodium channel. by using antipeptide antibodies against poma or pomb, we carried out immunoprecipitation to verify whether these proteins form a complex and examined the in vivo stabilities of poma and pomb. as a result, we could demonstrate that poma and pomb functionally interact with eac ... | 1999 | 10438787 |
| random mutagenesis of the poma gene encoding a putative channel component of the na(+)-driven polar flagellar motor of vibrio alginolyticus. | poma and pomb are integral membrane proteins and are essential for the rotation of the na(+)-driven polar flagellar motor of vibrio alginolyticus. on the basis of their similarity to mota and motb, which are the proton-conducting components of the h(+)-driven motor, they are thought to form the na(+)-channel complex and to be essential for mechanochemical coupling in the motor. to investigate poma function, random mutagenesis of the poma gene by using hydroxylamine was carried out. we isolated 3 ... | 1999 | 10439415 |
| active immunisation of black tiger prawn (penaeus monodon) against vibriosis in thailand. | mechanisms of host defence in crustaceae and vibriosis in shrimp and methods for its prevention are discussed as introduction. the own work deals with the development of a site-specific multivalent anti-vibrio vaccine and its application in thailand. the vaccine was produced in a continuous bioreactor system from field strains of vibrio alginolyticus, v. harveyi, v. parahaemolyticus and v. vulnificus, purified through ultrafiltration, and inactivated by formalin. it was applied to penaeus monodo ... | 1999 | 10486986 |
| on the in-vitro antimicrobial activity of oleuropein and hydroxytyrosol. | secoiridoides (oleuropein and derivatives), one of the major classes of polyphenol contained in olives and olive oil, have recently been shown to inhibit or delay the rate of growth of a range of bacteria and microfungi but there are no data in the literature concerning the possible employment of these secoiridoides as antimicrobial agents against pathogenic bacteria in man. in this study five atcc standard bacterial strains (haemophilus influenzae atcc 9006, moraxella catarrhalis atcc 8176, sal ... | 1999 | 10504039 |
| hybrid motor with h(+)- and na(+)-driven components can rotate vibrio polar flagella by using sodium ions. | the bacterial flagellar motor is a molecular machine that converts ion flux across the membrane into flagellar rotation. the coupling ion is either a proton or a sodium ion. the polar flagellar motor of the marine bacterium vibrio alginolyticus is driven by sodium ions, and the four protein components, poma, pomb, motx, and moty, are essential for motor function. among them, poma and pomb are similar to mota and motb of the proton-driven motors, respectively. poma shows greatest similarity to mo ... | 1999 | 10515922 |
| evolutionary changes reflected by the cellular amino acid composition. | comparison of the amino acid composition of cell-proteins using 17 amino acids has been used to investigate the biological evolution of organisms such as bacteria, blue-green alga, green alga, fungi, slime mold, protozoa and vertebrates. the degree of difference in the amino acid ratios between any two groups reflects the degree of divergency in biological evolution. the amino acid composition of the gram-negative bacteria (escherichia coli, klebsiella, proteus, and vibrio alginolyticus) was ide ... | 1999 | 10524278 |
| the effect of motility and cell-surface polymers on bacterial attachment. | recently it was shown that motility of vibrio alginolyticus facilitated cell attachment to glass surfaces. in the present study the same relationship between motility and cell attachment was confirmed for alcaligenes and alteromonas spp. these findings clearly answer a long-standing question: does motility facilitate attachment? however, they are contradictory to a general view on cell attachment that the energy barrier due to electrostatic repulsion between negatively charged bacterial cells an ... | 1999 | 10537201 |
| inhibitor studies of a new antibiotic, korormicin, 2-n-heptyl-4-hydroxyquinoline n-oxide and ag+ toward the na+-translocating nadh-quinone reductase from the marine vibrio alginolyticus. | a new antibiotic, korormicin, isolated from a marine bacterium pseudoalteromonas sp. f-420, was found to strongly inhibit the respiratory chain-linked na+-translocating nadh-quinone reductase (nqr) from the marine vibrio alginolyticus. similar to 2-n-heptyl-4-hydroxyquinoline n-oxide (hqno), korormicin specifically inhibited the na+-dependent reaction in the nqr complex that is directly coupled to the extrusion of na+ from the cells. both korormicin and hqno acted as purely noncompetitive inhibi ... | 1999 | 10549856 |
| sequencing and preliminary characterization of the na+-translocating nadh:ubiquinone oxidoreductase from vibrio harveyi. | the na(+)-translocating nadh: ubiquinone oxidoreductase (na(+)-nqr) generates an electrochemical na(+) potential driven by aerobic respiration. previous studies on the enzyme from vibrio alginolyticus have shown that the na(+)-nqr has six subunits, and it is known to contain fad and an fes center as redox cofactors. in the current work, the enzyme from the marine bacterium vibrio harveyi has been purified and characterized. in addition to fad, a second flavin, tentatively identified as fmn, was ... | 1999 | 10587447 |
| chronic diarrhea associated with vibrio alginolyticus in an immunocompromised patient. | 1999 | 10589925 | |
| mechanosensitive channel functions to alleviate the cell lysis of marine bacterium, vibrio alginolyticus, by osmotic downshock. | the mechanosensitive channel with large conductance of escherichia coli is the first to be cloned among stretch-activated channels. although its activity was characterized by a patch clamp method, a physiological role of the channel has not been proved. the marine bacterium, vibrio alginolyticus, is sensitive to osmotic stress and cell lysis occurs under osmotic downshock. we introduced an mscl gene into vibrio alginolyticus, and the mechanosensitive channel with large conductance functions was ... | 1999 | 10050752 |
| the polar flagellar motor of vibrio cholerae is driven by an na+ motive force. | vibrio cholerae is a highly motile bacterium which possesses a single polar flagellum as a locomotion organelle. motility is thought to be an important factor for the virulence of v. cholerae. the genome sequencing project of this organism is in progress, and the genes that are highly homologous to the essential genes of the na+-driven polar flagellar motor of vibrio alginolyticus were found in the genome database of v. cholerae. the energy source of its flagellar motor was investigated. we exam ... | 1999 | 10074090 |
| identification of vibrio parahaemolyticus strains at the species level by pcr targeted to the toxr gene. | the dna colony hybridization test with the polynucleotide probe for vibrio parahaemolyticus toxr gene was performed. all 373 strains of v. parahaemolyticus gave positive results, and the strains belonging to four other vibrio species including vibrio alginolyticus gave weakly positive results, suggesting that toxr sequence variation may reflect the phylogenetic relationships of vibrio species. we then established a toxr-targeted pcr protocol for the specific detection of v. parahaemolyticus. | 1999 | 10074546 |
| effects of changes in membrane sodium flux on virulence gene expression in vibrio cholerae. | the expression of several virulence factors of vibrio cholerae is coordinately regulated by the toxt molecule and the membrane proteins tcpp/h and toxr/s, which are required for toxt transcription. to identify proteins that negatively affect toxt transcription, we screened transposon mutants of v. cholerae carrying a chromosomally integrated toxt::lacz reporter construct for darker blue colonies on media containing 5-bromo-4-chlor-3-indolyl beta-d galactoside (x-gal). two mutants had transposon ... | 1999 | 10077658 |
| purification and partial characterization of a toxic serine protease produced by pathogenic vibrio alginolyticus. | an extracellular lethal toxin produced by vibrio alginolyticus strain swy originally isolated from diseased kuruma prawn (penaeus japonicus) was purified using the akta purifier system with hydrophobic interaction chromatography, anion exchange and gel filtration columns. the toxin is an alkaline serine protease, inhibited by phenyl methylsulphonyl fluoride (pmsf), antipain and shows maximal activity at ph 8 to 11, having a pi of 4.3 and a molecular weight of approximately 33 kd. the toxin was c ... | 1999 | 10624008 |
| [antibiotic resistance and plasmid profiles of vibrio isolates from cultured sparus sarba]. | a total of 51 potential pathogenic vibrios were isolated from moribund silver seabream sparus sarba, which were collected from local fish farms of hong kong. all the isolates were classified and identified as 7 species by the api 20 e system and the scheme of alsina & blanch. these species were vibrio alginolyticus (24 strains), vibrio vulnificus (12 strains), vibrio parahaemolyticus(7 strains), vibrio logei(4 strains), vibrio pelagius ii(2 strains), vibrio fluvialis (1 strains) and vibrio medit ... | 1999 | 12555529 |
| [the "cavernous sinus" (author's transl)]. | 1999 | 91130 | |
| remission in acute myeloblastic leukaemia. | 1999 | 91055 | |
| [improvement in the prognosis of rapid progressive glomerulonephritis by plasmapheresis treatment]. | 1999 | 2860543 | |
| [statistical analysis of the ecg r-r intervals in certain types of heart rhythm disorders]. | 1999 | 91156 | |
| [presence of an amphipatic segment in the b fragment of diphtheria toxin (proceedings)]. | 1999 | 93460 | |
| [evaluation of the restoration of micturition after transvesical adenomectomy of the prostate using a urodynamic flow measurement method]. | 1999 | 93823 | |
| epileptogenic effect of mianserin. | 1999 | 91069 | |
| plasma arginine vasopressin in diabetic ketoacidosis. | plasma arginine vasopressin (avp) was measured serially in 10 patients during the first 24 hours of treatment of diabetic ketoacidosis. avp was elevated in all cases initially, ranging from 4.0--122.0 pmol/l, the basal level in healthy hydrated subjects being 1.57 +/- 0.59 pmol/l (+/- 1 sd). the levels fell progressively during the course of treatment. while there was no evidence that the massive increases in avp noted in this study were associated with water overload it remains to be determined ... | 1999 | 103767 |
| substances modulating the secretion of acth by cultured anterior pituitary cells. | 1999 | 17552 | |
| tetralogy of fallot in adults. | 1999 | 91440 | |
| biochemical changes in the skin of mink with ehlers-danlos syndrome: increased collagen biosynthesis in the dermis of affected mink. | 1999 | 21930 | |
| chido, rodgers and c4. in vivo and in vitro coating of red blood cells, grouping and antibody detection. | c4 sucrose/low ionic strength (lis)-coated red blood cells (rbc) are excellent for the detection of the previously 'nebulous' antibodies, anti-chido and anti-rodgers, as well as for serum/plasma typing of these antigens by an inhibition technique. by enzyme treatment of such cells, it is confirmed that the ch and rg antigens reside on the c4d part of the c4 molecule. freshly taken rbc from normal individuals were examined with a sensitive auto-analyzer technique with anti-chido, anti-c4 and anti ... | 1999 | 91269 |
| [evaluation of blood-liquor barrier by protein gradients in congenital hydrocephalus in infancy]. | 1999 | 88669 | |
| ectopic pregnancy in southern iran: a statistical review of 96 cases. | a review is presented of 96 cases of ectopic pregnacy over a 7-year period. it is noted that in recent years the incidence of this problem has increased. inadequately diagnosed and treated pelvic inflammatory disease has been found to be the main predisposing factor (40%). the rate of extra-uterine gestation was 1:336 deliveries. in this study, previous fertility was high, and primigravidae constituted only 10.4% of the total. the effect of previous pelvic operations and pelvic infection is disc ... | 1999 | 15916 |
| histochemical study of orcein-positive hepatocellular material in paraffin sections of liver biopsy samples in the course of various liver diseases. | a histochemical study was carried out on orcein-positive hepatocellular material in 111 cases, 27 of which were stained positively by orcein. orcein-positive material was very frequently found in protracted viral hepatitis and in chronic active hepatitis, as well as in other liver diseases with or without cholestasis; it was absent in liver cirrhosis. in all the cases considered the orcein-positive material was mainly formed of proteins, rich in amino acids with sulfhydryl and disulphide radical ... | 1999 | 91188 |
| purification and properties of beta-n-acetylglucosaminidase from vibrio alginolyticus h-8. | beta-n-acetylglucosaminidase [ec 3.2.1.30] from vibrio alginolyticus h-8 was purified by column chromatography on deae-sepharose ff, phenyl-sepharose hp, superdex 200hr, and mono q. the molecular weight of the enzyme was estimated by sds-gel electrophoresis (sds-page) to be 75 kda. the pi was 4.6. the activity was inhibited by ag+, hg2+, iodoacetate, p-chloromercuribenzoate, and n-ethylmaleimide. | 1999 | 16232581 |
| cloning and sequencing of a chitinase gene from vibrio alginolyticus h-8. | a gene from vibrio alginolyticus h-8, encoding chitinase, designated as chitinase b, was cloned by the shot-gun method using puc118 and sequenced. the open reading frame consisted of 846 amino acids including a signal peptide. the molecular mass of the enzyme estimated based on the amino acid sequence data was 90 kda. the n-terminal amino acid sequence of the enzyme was different from that of chitinase c1 which we had previously reported. this cloned chitinase b was considered one out of four ch ... | 2000 | 16232787 |