Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
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| esed, a putative t3ss translocon component of edwardsiella tarda, contributes to virulence in fish and is a candidate for vaccine development. | edwardsiella tarda has a type iii secretion system (t3ss) essential for pathogenesis. esed, together with eseb and esec, has been suggested to form a putative t3ss translocon complex, although its further function is unclear. to investigate the physiological role of esed, a mutant strain of e. tarda was constructed with an in-frame deletion of the entire esed gene. one finding was that the ∆esed mutant decreased the secretion levels of esec and eseb proteins. additionally, the ∆esed mutant showe ... | 2010 | 20072793 |
| production of an anti-idiotypic antibody single chain variable fragment vaccine against edwardsiella tarda. | edwardsiella tarda is the pathogen responsible for edwardsiellosis, a serious infectious disease of freshwater and marine fish species, and currently recognized to be the species pathogenic for human. an anti-idiotypic monoclonal antibody (mab), 1e11, has been developed. it mimics the protective epitope of e. tarda and can prevent fish from infection of e. tarda. in this study, the correct variable heavy (vh) and variable light (vl) genes were obtained from 1e11 by using bioinformatics methods, ... | 2010 | 20119624 |
| identification and characterization of matrix metalloproteinase-13 sequence structure and expression during embryogenesis and infection in channel catfish (ictalurus punctatus). | matrix metalloproteinase-13 (mmp-13), referred to as collagenase-3, is a proteolytic enzyme that plays a key role in degradation and remodelling of host extracellular matrix proteins. the objective of this study was to characterize the mmp-13 gene in channel catfish, and to determine its pattern of expression in various healthy tissues and during embryogenesis. since mmp-13 has been shown to have importance in tissue remodelling and some pathological processes, we further studied its involvement ... | 2010 | 20060415 |
| scophthalmus maximus cystatin b enhances head kidney macrophage-mediated bacterial killing. | cystatins form a large family of cysteine protease inhibitors found in a wide arrange of organisms. studies have indicated that mammalian cystatins play important roles under both physiological and pathological conditions. however, much less is known about fish cystatins. in this report, we described the identification and analysis of a cystatin b homologue, smcytb, from turbot scophthalmus maximus. the open reading frame of smcytb is 300bp, which encodes a 99-residue protein that shares high le ... | 2010 | 20692286 |
| comparative study of the effects of aluminum adjuvants and freund's incomplete adjuvant on the immune response to an edwardsiella tarda major antigen. | edwardsiella tarda is a severe aquaculture pathogen that can infect many different fish species cultured worldwide. et49 is a major e. tarda antigen with weak immunoprotective potential. in this study, using et49 as an example vaccine, the adjuvanticity of freund's incomplete adjuvant (fia), aluminum hydroxide, and aluminum phosphate adjuvant were evaluated in a japanese flounder model. the results showed that the presence of fia, aluminum hydroxide, and aluminum phosphate adjuvant increased the ... | 2010 | 20006568 |
| the iron-cofactored superoxide dismutase of edwardsiella tarda inhibits macrophage-mediated innate immune response. | edwardsiella tarda is a gram-negative bacterium that can infect a wide variety of marine and freshwater fish and cause severe economic losses worldwide. with an aim to elucidate the virulence mechanism of e. tarda, we in this study cloned and analyzed the function of an iron-cofactored superoxide dismutase (fesod) from a pathogenic e. tarda strain tx01 isolated from diseased fish. fesod is 192-residue in length and contains domain structures that are conserved among iron/manganese superoxide dis ... | 2010 | 20732430 |
| characterization and expression analysis of the myeloid differentiation factor 88 (myd88) in rock bream oplegnathus fasciatus. | myeloid differentiation factor 88 (myd88) is a universal adaptor protein able to activate nuclear factor-kappa b (nf-κb) through interactions with interleukin-1 receptor (il-1r) and the toll-like receptors (tlrs), with the exception of tlr3. here, we describe the identification of myd88 from the rock bream fish oplegnathus fasciatus and its characterization based on gs-flx™ sequencing. the cdna of rock bream myd88 was found to be composed of 1626 bp, with an 867 bp open reading frame that encode ... | 2010 | 21152988 |
| characterization and expression analysis of a goose-type lysozyme from the rock bream oplegnathus fasciatus, and antimicrobial activity of its recombinant protein. | lysozyme (muramidase) represents an important defense molecule of the fish innate immune system. known for its bactericidal properties, lysozyme catalyzes the hydrolysis of β-(1,4)-glycosidic bonds between the n-acetyl glucosamine and n-acetyl muramic acid in the peptidoglycan layer of bacterial cell walls. in this study, the complete coding sequence of a g-type lysozyme (rbglyz) was identified in the oplegnathus fasciatus rock bream fish genome by means of multi-tissue normalized cdna pyroseque ... | 2010 | 21167286 |
| mutations in the gyrb, parc, and pare genes of quinolone-resistant isolates and mutants of edwardsiella tarda. | the full length genes gyrb (2,415 bp), parc (2,277 bp), and pare (1,896 bp) in edwardsiella tarda were cloned by pcr with degenerate primers based on the sequence of the respective quinolone resistance-determining region (qrdr), followed by elongation of 5' and 3' ends using cassette ligation-mediated pcr (clmp). analysis of the cloned genes revealed open reading frames (orfs) encoding proteins of 804 (gyrb), 758 (parc), and 631 (pare) amino acids with conserved gyrase/topoisomerase features and ... | 2010 | 21193831 |
| broad-host-range plasmids for red fluorescent protein labeling of gram-negative bacteria for use in the zebrafish model system. | to observe real-time interactions between green fluorescent protein-labeled immune cells and invading bacteria in the zebrafish (danio rerio), a series of plasmids was constructed for the red fluorescent protein (rfp) labeling of a variety of fish and human pathogens. the aim of this study was to create a collection of plasmids that would express rfp pigments both constitutively and under tac promoter regulation and that would be nontoxic and broadly transmissible to a variety of gram-negative b ... | 2010 | 20363780 |
| novel expression system for combined vaccine production in edwardsiella tarda ghost and cadaver cells. | to develop combined vaccine systems, we have generated edwardsiella tarda ghosts (etg) displaying a foreign protein on the outer membrane and also ed. tarda cadaver (etc) expressing a heterologous protein in the cytoplasm. green fluorescent protein (gfp) was used as a model foreign protein. a constitutive promoter (etpr c28-1) cloned newly from ed. tarda was used as a promoter for the expression of foreign protein. comparison of the strength of the new promoter with a commercially available cons ... | 2010 | 20369310 |
| differential regulation of sciaenops ocellatus viperin expression by intracellular and extracellular bacterial pathogens. | viperin is an antiviral protein that has been found to exist in diverse vertebrate organisms and is involved in innate immunity against the infection of a wide range of viruses. however, it is largely unclear as to the potential role played by viperin in bacterial infection. in this study, we identified the red drum sciaenops ocellatus viperin gene (sovip) and analyzed its expression in relation to bacterial challenge. the complete gene of sovip is 2570 bp in length and contains six exons and fi ... | 2010 | 20420911 |
| molecular cloning and expression study on toll-like receptor 5 paralogs in japanese flounder, paralichthys olivaceus. | toll-like receptor (tlr) 5 is responsible for the bacterial flagellin recognition in vertebrates. synergistic role of tlr 5 membrane form (tlr 5m) and tlr 5 soluble form (tlr 5s) have been reported from the study on rainbow trout. this system is regarded as the unique system in teleost fish. however, systemic response of tlr 5 genes in teleost fish has not been fully understood. hence, we cloned japanese flounder (paralichthys olivaseus) tlr 5m and tlr 5s genes and their expressions were analyze ... | 2010 | 20561590 |
| evaluation of four polymerase chain reaction primer pairs for the detection of edwardsiella tarda in turbot. | edwardsiella tarda is an important emergent pathogen in european aquaculture, causing several mortality events in turbot scophthalmus maximus cultures in recent years. here, we evaluated in parallel the specificity of 4 previously published pairs of primers, gyrbf1/gyrbr1, tardaf/ tardar, etfa and etfd, for the detection of 53 e. tarda strains isolated from different sources, as well as 18 representatives of related and unrelated bacterial species. on the basis of the obtained results, we select ... | 2010 | 20597430 |
| smc2p1, a c2 domain protein from scophthalmus maximus that binds bacterial pathogen-infected lymphocytes and reduces bacterial survival. | c2 domains are protein structural modules found in many eukaryotic proteins involved in signal transduction, membrane trafficking, and immune defense. most of the studied c2 domain-containing proteins are multi-domained in structure, in which the c2 domain is an independently folded motif and plays an essential role in calcium-dependent membrane-targeting. although c2 domains isolated from intact proteins have been studied for biological functions, no study on natural proteins containing c2 doma ... | 2010 | 20633652 |
| differential gene expression profiles in japanese flounder (paralichthys olivaceus) with different susceptibilities to edwardsiellosis. | edwardsiellosis, which is caused by edwardsiella tarda, a gram-negative bacterium, is one of the most serious problems in japanese flounder (paralichthys olivaceus) culture. in this study, we examined the immune responses at the molecular level of genetic groups of japanese flounder that are either susceptible or moderately susceptible to e. tarda infection using a cdna microarray which was spotted with approximately 2000 different genes. four different genetic groups of japanese flounder (group ... | 2010 | 20637288 |
| isolation and analysis of the vaccine potential of an attenuated edwardsiella tarda strain. | edwardsiella tarda is an important aquaculture pathogen that can infect a wide range of marine and freshwater fish worldwide. in this study, a modified e. tarda strain, tx5rm, was selected by multiple passages of the pathogenic e. tarda strain tx5 on growth medium containing the antibiotic rifampicin. compared to the wild type strain, the rifampicin-resistant mutant tx5rm (i) shows drastically increased median lethal dose and reduced capacity to disseminate in and colonize fish tissues and blood ... | 2010 | 20637307 |
| eseg, an effector of the type iii secretion system of edwardsiella tarda, triggers microtubule destabilization. | edwardsiella tarda is a gram-negative enteric pathogen that causes hemorrhagic septicemia in fish and both gastrointestinal and extraintestinal infections in humans. a type iii secretion system (t3ss) was recently shown to contribute to pathogenesis, since deletions of various t3ss genes increased the 50% lethal dose (ld(50)) by about 1 log unit in the blue gourami infection model. in this study, we report eseg as the first identified effector protein of t3ss. eseg shares partial homology with t ... | 2010 | 20855515 |
| construction and analysis of the immune effect of an edwardsiella tarda dna vaccine encoding a d15-like surface antigen. | edwardsiella tarda is a severe aquaculture pathogen with a broad host range that includes both humans and animal. in a previous study, we have identified in e. tarda a d15-like surface antigen, esa1, which, when used as a recombinant subunit vaccine, is able to induce protective immunity in japanese flounder (paralichthys olivaceus) against e. tarda challenge. in this study, we examined further the immunoprotective potential of esa1 as a dna vaccine. for this purpose, the dna vaccine plasmid pce ... | 2010 | 21059395 |
| galectin-1 (ofglec-1) from rock bream (oplegnathus fasciatus): identification, characterization, gene expression and functional properties. | lectins may function as pattern-recognition receptors (prrs) and play important roles in immune responses. in this study, a novel galectin-1 (designated ofglec-1) gene was identified from rock bream, oplegnathus fasciatus, cdna library. the complete cdna sequence of the ofglec-1 gene is 993 base pairs in length, and encodes 135 amino acids. ofglec-1 shares a highly conserved carbohydrate recognition domain with galectin-1 from other fishes. the mrna expressions of ofglec-1 in healthy and bacteri ... | 2010 | 21073891 |
| comparative analysis of the phenotypic characteristics of high- and low-virulent strains of edwardsiella tarda. | edwardsiella tarda is a causative agent of edwardsiellosis in freshwater and marine fish. extracellular enzymic, haemolytic, hydrophobic and serum resistance activities, haemagglutination, autoagglutination and siderophores of high- and low- virulent e. tarda strains were examined. the results revealed different haemagglutination, autoagglutination, haemolytic, hydrophobic and serum resistance activities in different strains. analysis of extracellular proteins (ecps) and outer membrane proteins ... | 2010 | 21091725 |
| molecular cloning and expression analysis of interferon regulatory factor 10 (irf10) in japanese flounder, paralichthys olivaceus. | interferons (ifns) are important for the defense against intracellular pathogens. interferon regulatory factor 10 (irf10) is a transcript factor involved in regulating ifn signaling induced by virus infections in birds, but little is know of its role in the immune response in non-avian vertebrates. here, we identified and characterized the irf10 gene and examined its expression pattern in a teleost fish, japanese flounder, paralichthys olivaceus. japanese flounder irf10 (poirf10) gene is a singl ... | 2010 | 20883793 |
| characterization and gene expression of transcription factors, pu.1 and c/ebpα driving transcription from the tumor necrosis factor α promoter in japanese flounder, paralichthys olivaceus. | both pu.1 and c/ebpα transcription factors play important roles in myeloid development and inflammatory response. these transcripts were cloned from the japanese flounder (paralichthys olivaceus) and were highly conserved with those of other vertebrates. pu.1 mrna was mainly expressed in lymphoid tissues while c/ebpα mrna was widely expressed in all tissues examined. higher levels of pu.1 mrna were expressed in the igm(+) cells of both pbl and kl, while c/ebpα expression was higher only in the i ... | 2010 | 20951726 |
| identification of an edwardsiella tarda surface antigen and analysis of its immunoprotective potential as a purified recombinant subunit vaccine and a surface-anchored subunit vaccine expressed by a fish commensal strain. | edwardsiella tarda is the etiological agent of edwardsiellosis, a systematic disease that affects a wide range of marine and freshwater fish cultured worldwide. in order to identify e. tarda antigens with vaccine potential, we in this study conducted a systematic search for e. tarda proteins with secretion capacity. one of the proteins thus identified was esa1, which contains 795 amino acid residues and shares extensive overall sequence identities with the d15-like surface antigens of several ba ... | 2010 | 20673823 |
| immune response of japanese flounder paralichthys olivaceus to outer membrane protein of edwardsiella tarda. | the humoral and cellular immune responses of japanese flounder, paralichthys olivaceus, were investigated following intraperitioneal injection with outer membrane protein (omp) of edwardsiella tarda in freund's incomplete adjuvant (fia). the specific serum antibody titre against omp of e. tarda were measured using elisa for 14 weeks, and the total serum antibody concentrations were also determined according to the sandwich elisa standard model constructed using purified igm. both of the specific ... | 2010 | 19944169 |
| proteomic analysis of outer membrane proteins of edwardsiella tarda. | the purpose of this study was to identify outer membrane proteins (omps) of edwardsiella tarda. | 2010 | 20002913 |
| extracellular products from virulent strain of edwardsiella tarda stimulate mouse macrophages (raw264.7) to produce nitric oxide (no) and tumor necrosis factor (tnf)-alpha. | we have previously found that high virulent strain (nuf251) of edwardsiella tarda, but not low virulent strain (nuf194), was able to survive and multiply within japanese flounder (paralichthys olivaceus) peritoneal macrophages. further studies demonstrated that nuf251 induced much higher levels of no and tnf-alpha productions than nuf194 in both japanese flounder peritoneal macrophages and mouse macrophage cell line raw264.7. in this study, we examined the effects of extracellular products (ecp) ... | 2010 | 20633653 |
| the efficacy of five avirulent edwardsiella tarda strains in a live vaccine against edwardsiellosis in japanese flounder, paralichthys olivaceus. | we evaluated the tissue persistence and live vaccine efficacy of five avirulent edwardsiella tarda strains (e22, su100, su117, su138, and su244) isolated from the japanese eel (anguilla japonica) and from the environment. the live vaccines, containing a single strain, were injected intraperitoneally into japanese flounder (paralichthys olivaceus). viable bacteria from all the strains (excluding su100) were recovered from trunk-kidney tissue 28 d post-injection. japanese flounder inoculated with ... | 2010 | 20633656 |
| dissection and localization of the immunostimulating domain of edwardsiella tarda flic. | bacterial flagellin is known to induce potent immune response in vertebrate systems via the toll-like receptor (tlr) 5. as a result, flagellin has been studied extensively as a vaccine adjuvant. in a previous study, we examined the vaccine and adjuvant potentials of the flagellin (flic) of the fish pathogen edwardsiella tarda. we found that e. tarda flic induced low protective immunity by itself but could function as a molecular adjuvant and potentiate the specific immune response induced by the ... | 2010 | 20580470 |
| defensin of the zebra mussel (dreissena polymorpha): molecular structure, in vitro expression, antimicrobial activity, and potential functions. | a 409 bp full length defensin gene was cloned and sequenced based on an expressed sequence tag (est) obtained from a normalized zebra mussel (dreissena polymorpha) foot cdna library developed in our laboratory. the d. polymorpha defensin (dpd) gene encoded a peptide with 76 amino acid residues. the mature dpd contains 54 amino acids with a fully functional insect defensin a domain. homologue searching against genbank database suggested that this dpd was phylogenetically close to defensins from a ... | 2010 | 20537393 |
| identification and analysis of a sciaenops ocellatus isg15 homologue that is involved in host immune defense against bacterial infection. | isg15 is an interferon-stimulated gene that encodes a ubiquitin-like protein. isg15 homologues have been identified in a number of fish species, some of which are known to be regulated at expression level by virus infection and lipopolysaccharide (lps) treatment. however, the relationship between isg15 and live bacterial infection has not been investigated in piscine models. in this study, an isg15 homologue, soisg15, was identified from red drum sciaenops ocellatus and analyzed at expression an ... | 2010 | 20385242 |
| protection of red sea bream pagrus major against red sea bream iridovirus infection by vaccination with a recombinant viral protein. | megalocytivirus infections cause serious mass mortality in marine fish in east and southeast asian countries. in this study the immunogenicity of crude subunit vaccines against infection by the megalocytivirus rsiv was investigated. three capsid proteins, 18r, 351r and a major capsid protein, were selected for use as crude subunit vaccines. high homology among megalocytivirus types was found in the initial sequence examined, the 351r region. red sea bream (pagrus major) juveniles were vaccinated ... | 2010 | 20236423 |
| structural characteristics and antioxidant activities of the extracellular polysaccharides produced by marine bacterium edwardsiella tarda. | two water-soluble extracellular polysaccharides, etw1 and etw2, were isolated from the marine bacterium edwardsiella tarda by ion-exchange and size-exclusion chromatography, and their structures were investigated. etw1 and etw2 are mannans, with molecular weights of about 29 and 70kda, respectively. the main chain of the polysaccharides consists of (1-->3)-linked mannose residues, and branched mannose linkages were also detected. the branch points are located at the c-2 and c-6 positions of the ... | 2010 | 20156684 |
| analysis of the vaccine potential of a natural avirulent edwardsiella tarda isolate. | edwardsiella tarda is a severe aquaculture pathogen that can infect many fish species and cause a systematic disease called edwardsiellosis, which can lead to high mortality under certain conditions. currently, most vaccine candidates against edwardsiellosis are based on pathogenic e. tarda strains, which can be a concern in some cases. in this study, the vaccine potential of a natural e. tarda isolate, atcc 15947, was examined in a japanese flounder model. atcc 15947 was found to be relatively ... | 2010 | 20109592 |
| analysis of edwardsiella tarda degp, a serine protease and a protective immunogen. | edwardsiella tarda is a severe aquaculture pathogen with a broad host range that includes humans, animal, and fish. a gene (degp(et)) encoding a degp homologue was cloned from tx01, a pathogenic e. tarda strain isolated from diseased fish. degp(et) shares high sequence identities with the degp proteins of several bacterial species. functional analyses showed that degp(et) could complement the temperature-sensitive phenotype of an escherichia coli degp null mutant. expression of degp(et) in tx01 ... | 2010 | 20060910 |
| production of monoclonal antibodies specific to major outer membrane protein of edwardsiella tarda. | edwardsiella tarda is an important cause for hemorrhagic septicemia in fish and gastro and extra-intestinal infections in humans. monoclonal antibodies (mabs) were produced against outer membrane proteins (omps) of e. tarda et-7, isolated from diseased snakehead (ophiocephalus punctatus). two stable hybridoma clones, designated as 3f10 and 2c3 mabs were found to be potentially specific for e. tarda by indirect enzyme linked immunosorbent assay (elisa). these mabs recognized major immunogenic omp ... | 2010 | 18804863 |
| dna adenine methylase is involved in the pathogenesis of edwardsiella tarda. | edwardsiella tarda is a serious aquaculture pathogen that can infect many cultured fish species. the aim of this study was to investigate the potential importance of dna adenine methylase (dam) in e. tarda pathogenesis. the e. tarda dam gene (dam(et)) was cloned from a pathogenic strain, txd1, isolated from diseased fish. dam(et) shares high (70.2%) sequence identity with the dam proteins of yersinia enterocolitica and several other bacterial species. recombinant dam(et) is able to complement a ... | 2010 | 19781866 |
| isolation of faecal coliform bacteria from the american alligator (alligator mississippiensis). | to determine whether american alligators (alligator mississippiensis) are an unrecognized poikilothermic source of faecal coliform and/or potential pathogenic bacteria in south carolina's coastal waters. | 2010 | 19735329 |
| transgenic zebrafish eggs containing bactericidal peptide is a novel food supplement enhancing resistance to pathogenic infection of fish. | zebrafish (danio rerio) was used as a bioreactor to produce bovine lactoferricin (lfb), which has wide-ranging antimicrobial activity. we constructed an expression plasmid in which lfb was fused with green fluorescent protein (gfp) and driven by zebrafish beta-actin promoter. after microinjection, six transgenic founders were screened on the basis of gfp appearance. among them, a stable zbl-5 line was selected by the ubiquitous and strong expression of gfp. using pcr and western blot analysis, w ... | 2010 | 19944764 |
| verification of an edwardsiella ictaluri-specific diagnostic pcr. | to verify the specificity of a pcr assay for the identification and diagnosis of edwardsiella ictaluri. | 2010 | 20002568 |
| detection of different quorum-sensing signal molecules in a virulent edwardsiella tarda strain ltb-4. | the aim of this study was to elucidate the potential quorum-sensing (qs) signal molecules of an emerging pathogen (edwardsiella tarda strain ltb-4) of cultured turbot (scophthalmus maximus). | 2010 | 19548884 |
| analysis of the effect of copper on the virulence of a pathogenic edwardsiella tarda strain. | to investigate the effect of copper on the virulence of edwardsiella tarda. | 2010 | 19912523 |
| immune responses and expression profiles of some immune-related genes in indian major carp, labeo rohita to edwardsiella tarda infection. | edwardsiella tarda is an important gram-negative bacterium that causes systemic infections in a wide range of hosts including fish. the pathogenic mechanisms in this disease are still poorly understood in fish. indian major carp, labeo rohita were intraperitoneally challenged with a pathogenic isolate of e. tarda to measure sequential changes in immunity level. a significant decrease in the superoxide production, myeloperoxidase, alternative complement activity, total protein levels and antiprot ... | 2010 | 20045061 |
| the history of the use of vacuum extraction. | 2010 | 3047531 | |
| sequentially alternating hormone chemotherapy with high-dose medroxy-progesterone acetate and low-dose epirubicin for the treatment of hormone-resistant metastatic prostatic cancer. | thirty patients with hormone-resistant metastatic progressive prostatic carcinoma were treated with sequentially alternating hormone chemotherapy. they were given 1,000 mg medroxyprogesterone acetate (mpa) orally for 26 days followed by intravenous doses of 25 mg/m2 epirubicin weekly for 4 weeks. the median duration of the treatment was 29 weeks (range 8-84). in 2 patients a more than 50% reduction in the size of measurable lymph node metastases was observed and in 2 others skeletal metastases d ... | 2010 | 2975221 |
| hemobilia: a cause of false-negative ductal dilatation. | 2010 | 3095397 | |
| the antihypertensive effect of angiotensin-converting enzyme inhibition: a multifaceted mechanism of action. | 2010 | 3030977 | |
| [role of laparoscopic examination in the diagnosis and control of treatment results in early and less advanced forms of endometriosis]. | 2010 | 2956633 | |
| nocturnal adrenal suppression in children inhaling beclomethasone dipropionate. | 2010 | 2872406 | |
| [current trends in the development of the science of coagulation abroad]. | 2010 | 2996966 | |
| fine needle aspiration cytology of recurrent ectopic meningioma. | fine needle aspiration was performed on a recurrent extracranial meningioma in the left pterygomaxillary and temporal fossas in a 39-year-old woman. cytologic and electron microscopic study of the aspirate and comparison to the previously resected specimen proved the recurrent nature of the lesion. the cytologic and ultrastructural findings in meningiomas are discussed with special consideration of the differential diagnosis in extracranial sites. | 2010 | 2916374 |
| acute leukemia with extramedullary presentation and mixed myeloid and lymphoid expression. | 2010 | 3153242 | |
| macrophage colony-stimulating factor (m-csf) enhances the capacity of murine macrophages to secrete oxygen reduction products. | the capacity of macrophage colony-stimulating factor (m-csf) to enhance respiratory burst activity in peritoneal macrophages was measured. macrophages incubated for 48 hr or more with concentrated l cell-conditioned medium as a source of m-csf released two to three times as much o2- in response to pma as did unexposed macrophages. stimulation was noted at concentrations of colony-stimulating activity from 0.1 to 2000 u/ml and was maximal at 10 to 100 u/ml. purified, endotoxin-free csf enhanced s ... | 2010 | 2991376 |
| some analyses of forgetting of pictorial material in amnesic and demented patients. | three experiments evaluating the rate of forgetting of pictorial stimuli in amnesic, demented, and normal control subjects are reported. when prolonged exposures of pictorial material were used to equate recognition performance after a 10-min delay, alcoholic korsakoff (ak) patients and patients with huntington's disease (hd) demonstrated normal rates of forgetting over a 1-week period. in addition to replicating previous findings with ak patients, these results are consistent with recent sugges ... | 2010 | 2941450 |
| [problems of conservative dental treatment of patients with long-term dialysis treatment and after transplantation of the kidneys]. | 2010 | 2978595 | |
| [schistosomiasis haematobia (report of 22 cases)]. | 2010 | 2976322 | |
| a possible pain control system in a non-mammalian vertebrate (a lizard, gekko gecko). | in a lizard (gekko gecko) the anterograde tracer pha-l was microiontophoretically applied to the predominantly serotonergic nucleus raphes inferior. extensive spinal projections from the rostral magnocellular part of this nucleus were demonstrated to the superficial layers of the dorsal horn and to the intermediate zone, more sparsely to the ventral horn. but, in addition, retrogradely labeled neurons were found in and just below a periventricular cell group in tegmentum mesencephali, i.e. the l ... | 2010 | 2831478 |
| hemobilia due to ruptured hepatic artery aneurysm. | 2011 | 3235180 | |
| [status quo in lipid diagnosis from the internal medicine viewpoint. discussion of the contribution of the same title by w. reuter in z. gesamte inn. med. 43 (1988) 71-74]. | 2011 | 3213119 | |
| effect of synthetic thrombin inhibitor (md805) as an alternative drug on heparin induced thrombocytopenia during hemodialysis. | 2011 | 3194891 | |
| karyological studies in coris julis (pisces, labridae). | in the present investigation the diploid number 2n = 48 (nf = 58) has been determined for females, primary males, and secondary males of coris julis from the gulf of palermo. differentiated sex chromosomes have not been observed in the population under study. | 2011 | 3234765 |
| [low doses of subcutaneous cytosine arabinoside in the treatment of patients with myelodysplastic syndromes]. | 2011 | 3165541 | |
| compulsory continuing dental education. | 2011 | 3166118 | |
| a prolonged decline in the incidence of necrotizing enterocolitis after the introduction of a cautious feeding regimen. | 2011 | 3144829 | |
| court-ordered vs. voluntary treatment of abusive and neglectful parents. | the effects of voluntary vs. court-mandated participation in a comprehensive child abuse and neglect treatment program were compared. the treatment program offered therapeutic family day care for the children of preschool age, group sessions for the parents, and home treatment for the entire family. the impact of the program was noted in the quality of the affective interaction between parents and children and disciplinary methods used, among other measures. families in voluntary and court-order ... | 2011 | 3365581 |
| [drug analysis using thin layer chromatography. 2. densitometer for transmission measurements]. | 2011 | 4700925 | |
| darkroom diseases and how to combat them. | 2011 | 3112384 | |
| the two dps of edwardsiella tarda are involved in resistance against oxidative stress and host infection. | dna-binding protein from starved cells (dps) is a member of ferritin-like proteins that exhibit properties of nonspecific dna binding and iron oxidation and storage. although studies of dps from many bacterial species have been reported, no investigations on dps from fish pathogens have been documented. in this study, we examined the biological function of two dps proteins, dps1 and dps2, from edwardsiella tarda, an important fish bacterial pathogen that can also infect humans. dps1 and dps2 are ... | 2011 | 21907291 |
| edwardsiella tarda-a case report. | edwardsiella tarda, a bacterium associated with fresh water ecosystems, can cause life-threatening illnesses in susceptible hosts. to date, very few cases of neonatal edwardsiella tarda sepsis have been reported in the literature, none from india. the author reports a 4-d-old preterm with e. tarda septicemia. | 2011 | 22012143 |
| two-component phob-phor regulatory system and ferric uptake regulator sense phosphate and iron to control virulence genes in type iii and vi secretion systems of edwardsiella tarda. | inorganic phosphate (p(i)) and iron are essential nutrients that are depleted by vertebrates as a protective mechanism against bacterial infection. this depletion, however, is sensed by some pathogens as a signal to turn on the expression of virulence genes. here, we show that the phob-phor two-component system senses changes in p(i) concentration, whereas the ferric uptake regulator (fur) senses changes in iron concentration in edwardsiella tarda ppd130/91 to regulate the expression of type iii ... | 2011 | 21953460 |
| determination of the heterogeneous interactome between edwardsiella tarda and fish gills. | edwardsiellosis caused by edwardsiella tarda is a frequent occurrence throughout the world and has resulted in extensive losses in aquaculture. however, information regarding to protein-protein interaction between the pathogenic cells and host is not available although the portal of entry of the pathogen is determined. in this study, fish gill and bacterial pull-down approaches were used to isolate both bacterial outer membrane proteins that bind to gills and fish gill proteins that interact wit ... | 2011 | 22079874 |
| study on the immune enhancement of different immunoadjuvants used in the pentavalent vaccine for turbots. | in this study, we investigated the immune enhancing effects of different adjuvants used in a pentavalent vaccine for turbots. the pentavalent vaccine consisted of inactive bacterial cells from five common pathogenic strains (vibrio anguillarum, vibrio scophtalmi, edwardsiella tarda, vibrio harveyi and vibrio alginolyticus) and the adjuvants were astragalus polysaccharides (aps), propolis, and the freund's complete adjuvant (fca). turbots were immunized with the pentavalent vaccine alone or with ... | 2011 | 22178783 |
| an inflammatory cc chemokine of cynoglossus semilaevis is involved in immune defense against bacterial infection. | chemokines are a family of small cytokines that regulate leukocyte migration. based on the arrangement of the first two cysteine residues, chemokines are classified into four groups called cxc(+¦), cc(+¦), c, and cx(3)c. in this study, we identified a cc chemokine, cscck1, from half-smooth tongue sole (cynoglossus semilaevis) and analyzed its biological activity. the deduced amino acid sequence of cscck1 contains 111 amino acid residues and is phylogenetically belonging to the ccl19/21/25 group ... | 2011 | 21723394 |
| a novel immune-related gene, microtubule aggregate protein homologue, is up-regulated during ifn-+¦-related immune responses in japanese flounder, paralichthys olivaceus. | delayed-type hypersensitivity (dth) response mediated by antigen-specific th1 cells is used as a test to detect exposure to tuberculosis in humans. japanese flounder (paralichthys olivaceus) microtubule aggregate protein homologue (pomtap) was identified as a gene strongly induced during fish dth response. in this study, pomtap gene was cloned and its expression profile was analyzed. the pomtap gene has a transcriptional regulatory region that includes two interferon-stimulated response elements ... | 2011 | 21824491 |
| molecular cloning and expression analysis of two distinct f-type lectins from the rock bream, oplegnathus fasciatus. | several lectin families characterized by distinct signature sequence motifs and structural folds, such as c-type, peptidoglycan recognition protein, ficolin, pentraxins, and most recently galectins, have been implicated in immune surveillance. in this study, two distinct f-type lectins rbftl-1 and rbftl-2, from the rock bream (oplegnathus fasciatus), were identified and their expression was analyzed. the full-length cdna of rbftl-1 was composed of 1204bp with a 945-bp open reading frame (orf) th ... | 2011 | 21683734 |
| adverse reaction of acetaminophen as an alternative analgesic in a.a.s. triad. | the syndrome of a.a.s. triad consisting of an intolerance to analgesics such as aspirin, bronchial asthma and nasal polyps, is every day more frequent. during the last years, the use of acetaminophen as an analgesic-antipyretic alternative in patients with a.a.s. triad has been imperative. however, there are already publications that show reversible bronchial obstruction symptoms similar to the ones produced by a.a.s., though of less intensity. a study was performed in 32 patients diagnosed of a ... | 2011 | 3147588 |
| [bloemendaal, the hague]. | 2011 | 3145626 | |
| systematic mutation analysis of two-component signal transduction systems reveals esra-esrb and phop-phoq as the major virulence regulators in edwardsiella tarda. | edwardsiella tarda is a gram-negative broad-host-range pathogen that causes hemorrhagic septicemia in many commercially important fish species. its ability to adapt to and thrive in diverse environments outside and inside of its hosts prompts us to investigate the roles of the previously identified 33 putative two-component signal transduction systems (tcss) in e. tarda. in this work, we successfully constructed deletion mutations in each of the response regulator genes, suggesting that none of ... | 2011 | 22227416 |
| mutation of tryptophanase gene tnaa in edwardsiella tarda reduces lipopolysaccharide production, antibiotic resistance and virulence. | a fish pathogen edwardsiella tarda ltb-4 produced various indole alkaloids, including indole, 2-(1h-indol-3-yl)ethanol, 4-di(1h-indol-3-yl)methylphenol, tri(1h-indol-3-yl)methane and 2-[2,2-bis(1h-indol-3-yl)]ethylphenylamine. indole was the most abundant among these indole alkaloids. e. tarda ltb-4 produced indoles during its whole growth phase and maintained a high level (around 35.5 µm) during the stationary phase. the relevant tryptophanase (tnaa) gene tnaa was cloned from ltb-4 and conditio ... | 2011 | 23761341 |
| immunogenicity of pressure inactivated edwardsiella tarda bacterin to anguilla japonica (japanese eel). | japanese eel anguilla japonica were immunized with inactivated edwardsiella tarda bacterin preparations (formalin-killed cells, fkc (0.4%), formalin with heat-killed cells, fhkc (0.1% and 70 degreesc for 10 min), heat-killed cells, hkc (70 degrees c for 15 min), potassium chloride-killed cells, kkc (0.6%), tannic acid-killed cells, tkc (0.9%), citric acid-killed cells, cakc (0.9%), pressure-killed cells, pkc (600 psi for 5 min) and electric current-killed cells, eckc (100 ma at 12 v dc for 5 sec ... | 2011 | 22303581 |
| economic rights of the institutionalized mentally disabled. | 2011 | 3319919 | |
| effects of cost sharing on physiological health, health practices, and worry. | in a randomized trial of the effects of medical insurance on spending and the health status of the nonaged, we previously reported that patients with limited cost sharing had approximately one-third less use of medical services, similar general self-assessed health, and worse blood pressure, functional far vision, and dental health than those with free care. of the 20 additional measures of physiological health studied here on 3,565 adults, people with cost sharing scored better on 12 measures a ... | 2011 | 3119520 |
| delays in diagnosing and treating bladder cancer. | 2011 | 3133024 | |
| silicone as a trace contaminant in laboratory solvents. | this paper examines a suite of lipid solvents for their utility as solvents for silicone analysis by raman spectroscopy, and discusses the observation that off-the-shelf reagents could be contaminated by silicone during manufacture. of the solvents examined, only chloroform was transparent in those spectral regions (chiefly 500 cm-1) in which silicone exhibits its raman spectrum. also, the chloroform examined showed evidence of silicone contamination. therefore, chloroform is suggested as a suit ... | 2011 | 3212449 |
| combined bone marrow and gallium imaging. differentiation of osteomyelitis and infarction in sickle hemoglobinopathy. | the clinical records and scintigrams of patients with sickle hemoglobinopathy who underwent combined tc-99m bone marrow imaging and ga-67 imaging to differentiate osteomyelitis from bony infarction were reviewed. of 18 paired examinations in 15 patients, osteomyelitis was diagnosed correctly in six cases; in all six, gallium uptake at the symptomatic site was incongruently increased relative to the bone marrow activity. of the 12 episodes of infarction, 11 showed congruent activity on both tc-99 ... | 2011 | 3165313 |
| [diagnosis and therapeutic control of gonorrhea. effects and costs]. | 2011 | 3787810 | |
| brain death sans frontières. | 2011 | 3127708 | |
| melanoma antigen expression and metastatic ability of mutant b16 melanoma clones. | the biological functions of murine melanoma-associated antigens recognized by monoclonal antibodies (mabs) (m562, m622 and m2590) were examined by using mutant clones which differed in their degree of expression of these antigens. four clones of high expressors of 3 types of antigen (mea group), 5 clones of low or non-expressors of m562- and m622-recognizing antigens (meb group) and 4 clones of non-expressor of gm3 recognized by m2590 (mec group) were used. attachment of these clones to componen ... | 2011 | 3053466 |
| dermal, subcutaneous, and tendon xanthomas: diagnostic markers for specific lipoprotein disorders. | many patients with lipoprotein disorders are at increased risk for the development of premature atherosclerosis and, less commonly, other disorders that cause systemic morbidity. in some of these patients, xanthomas also develop and provide cutaneous markers for the lipoprotein disorder. as advances in molecular biology refine our understanding of lipoprotein metabolism, it has become increasingly clear that several types of xanthomas are associated with specific disease states. this article pre ... | 2011 | 3042820 |
| [multilevel structure isolation using modules based on the example of thoracic radiography]. | conventional chest radiographs were digitized and processed by multilevel band pass filtering. by this procedure different structural components are isolated according to their local spatial frequencies. in chest radiographs a textural component can be separated from a skeletal component. evaluation of radiological images is facilitated because changes can be looked for at relevant levels. a reconstruction of the image is possible, using different weighting factors to enhance interesting structu ... | 2011 | 3025963 |
| muscle function in women with rheumatoid arthritis--the influence of glucocorticosteroids. a clinical and morphological investigation. | 2011 | 3187461 | |
| vasogenic facial pain (cluster headache). | 8 cases of vasogenic facial pain are presented with delineation of differentiating clinical features. these vasogenic syndromes include classic episodic cluster headache, chronic cluster headache, and indomethacin responsive chronic cluster headache. open clinical trials employing inhalation o2 therapy as an abortive treatment strategy utilized in combination with prophylactic nifedipine therapy proved highly efficacious in controlling pain among most classic and chronic cluster headaches. a sin ... | 2011 | 3104492 |
| hospital visiting hours: time for improvement. | in a survey of hospital visiting hours in general medical and surgical wards throughout the united kingdom a pattern of visiting emerged showing that many hospitals still have very restricted visiting. a quarter of the 404 hospitals that responded permitted visiting for an average of two hours a day or less. in contrast, just over a third of hospitals allowed visiting for more than five hours a day. there was no relation between visiting times and type of hospital, but a striking regional variat ... | 2011 | 3133059 |
| morbidity and mortality of carotid endarterectomy under local anesthesia: a retrospective study. | it has been shown that carotid endarterectomy reduces the incidence of stroke in patients with symptomatic extracranial occlusive vascular disease in the absence of major perioperative complications such as stroke or death. we present a retrospective study of 106 carotid endarterectomies performed under local anesthesia in 100 patients in whom transient ischemic attack (tia) or minor stroke had occurred. nonfatal stroke occurred in 2%, and tia occurred in 1%. there was no perioperative mortality ... | 2011 | 3059211 |
| hepatotoxic and hepatoprotective potential of histamine (h2)-receptor antagonists. | 2011 | 3195618 | |
| impact of pharmacist intervention on oral theophylline therapy in adult inpatients. | the impact of clinical pharmacy services on the utilization of oral theophylline therapy was evaluated in a ten-week study involving 138 adult inpatients. the study initially involved an independent prospective five-week audit of theophylline use, in which clinical pharmacists monitored theophylline therapy and any interventions were designed so as not to influence future actions taken by medical officers with regard to oral theophylline therapy. the second part of the study involved active inte ... | 2011 | 3428141 |
| cd of ethidium bromide complexes with normal and electrophoretically anomalous dna restriction fragments. | 2011 | 3207850 | |
| metasternal gland secretion of the locust tree borer, megacyllene robiniae (coleoptera: cerambycidae). | 1. the volatile components of metasternal gland extracts of male and female megacyllene robiniae have been analyzed by gas chromatography-mass spectroscopy. 2. the major component was identified as 2-(1,3-hexadien-1-yl)-5-methyltetrahydrofuran, a new natural product. 3. 1-phenylethanol is present only in male extracts. 4. acetates of hexadecanol and octadecanol are also present. | 2011 | 3224513 |
| microarray technology is an effective tool for identifying genes related to the aquacultural improvement of japanese flounder, paralichthys olivaceus. | molecular techniques are now essential for discovering new functional genes for the improvement of cultured marine organisms. such techniques can be used to obtain expressed sequence tags (est) and, through the use of microarrays, the entire transcriptome. for example, microarrays can be used to reveal biomarkers of health conditions, nutrient changes and immune response in fish and shellfish. est-based microarray chips were constructed for cultured fish and shellfish species including japanese ... | 2011 | 20685186 |
| type vi secretion regulation: crosstalk and intracellular communication. | pathogens use type vi secretion systems (t6sss) to transport proteins into the environment or host cells in response to external stimuli. t6sss are tightly regulated together with other virulence determinants such as type iii secretion systems, quorum sensing (qs), and flagella synthesis. five pathogens (salmonella enterica, edwardsiella tarda, aeromonas hydrophila, vibrio cholerae, and pseudomonas aeruginosa) are examined for crosstalk proteins (global regulators) that connect t6sss to other vi ... | 2011 | 20971679 |