Publications

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cystathionine as a precursor of methionine in escherichia coli and aerobacter aerogenes.balish, edward (argonne national laboratory, argonne, ill.), and stanley k. shapiro. cystathionine as a precursor of methionine in escherichai coli and aerobacter aerogenes. j. bacteriol. 92:1331-1336. 1966.-cystathionine has been shown to be a precursor of methionine biosynthesis in escherichia coli and aerobacter aerogenes. a double enzyme assay was developed to show the formation of homocysteine from cystathionine. the results obtained support the concept that cystathionine serves as a precur ...19665332398
differentiation of escherichia coli and aerobacter aerogenes by gas liquid chromatography. 19665336209
metabolic injury to bacteria. i. effect of freezing and storage on the requirements of aerobacter aerogenes and escherichia coli for growth. 19664958829
bacteriostatic action of progesterone on staphylococci and other microorganisms.yotis, william (loyola university, hines, ill.), and ronald stanke. bacteriostatic action of progesterone on staphylococci and other microorganisms. j. bacteriol. 92:1285-1289. 1966.-progesterone has been examined in vitro for antibacterial activity against 10 microorganisms. turbidimetric and manometric techniques were used to assay the antibacterial activity of progesterone. the organisms tested consisted of staphylococcus aureus, s. epidermidis, gaffkya tetragena, bacillus subtilis, listeria ...19664958877
detection and identification of bacteria by gas chromatography.ether extracts of cultures of 29 strains representing 6 species of bacillus, and of individual strains of escherichia coli, aerobacter aerogenes, and pseudomonas aeruginosa were examined in a gas chromatograph by use of flame ionization and electron capture detectors. among the products detected were compounds with the chromatographic characteristics of acetic, propionic, and butyric acids, ethyl alcohol, diacetyl, acetoin, and 2,3-butanediol. the differences in peak areas of the various product ...19664959077
damaging effects of ethylenediaminetetra-acetate and penicillins on permeability barriers in gram-negative bacteria.1. the permeability barrier against benzylpenicillin has been found to be passive in four strains of penicillinase-producing gram-negative bacteria (three of klebsiella aerogenes and one of escherichia coli). 2. if the three k. aerogenes strains are grown in the presence of sub-inhibitory concentrations of benzylpenicillin, ampicillin or phenethicillin the resultant bacterial cells have deficient permeability barriers. concentrations of ampicillin or benzylpenicillin less than one-tenth of those ...19664961352
the pathway of myo-inositol degradation in aerobacter aerogenes. dehydrogenation and dehydration. 19665905122
the pathway of myo-inositol degradation in aerobacter aerogenes. ring scission. 19665905123
stereospecific d-glucokinase of aerobacter aerogenes. purification and properties. 19665908135
the composition of lipopolysaccharides of klebsiella aerogenes and aerobacter cloacae. 19665914339
effect of amino acids on the nitrogenase system of klebsiella pneumoniae.yoch, d. c. (south dakota state university, brookings), and r. m. pengra. effect of amino acids on the nitrogenase system of klebsiella pneumoniae. j. bacteriol. 92:618-622. 1966.-the effect of exogenous amino acids and the free amino acid pool on the synthesis of the nitrogenase system of klebsiella pneumoniae m5al (formerly aerobacter aerogenes m5al) was investigated. when an actively n(2)-fixing culture was used to inoculate a medium containing a limiting concentration of nh(4) (+), an induct ...19665922536
diaphorases from aerobacter aerogenes.bernofsky, carl (the university of kansas, kansas city), and russell c. mills. diaphorases from aerobacter aerogenes. j. bacteriol. 92:1404-1414. 1966.-five enzymes which catalyze the reduction of 2,6-dichlorophenol-indophenol by reduced nicotinamide adenine dinucleotide (nadh(2)) have been separated from sonic extracts of aerobacter aerogenes b199 by diethylaminoethyl (deae) cellulose chromatography. three major chromatographic fractions (enzymes i, ii, and iii) account for most of the activity ...19665924271
ultracentrifugal studies of the variation with physiological state of the ribosomes in aerobacter aerogenes. 19665928603
the purification and properties of d-allosephosphate isomerase of aerobacter aerogenes. 19665929157
pullulanase from aerobacter aerogenes; production in a cell-bound state. purification and properties of the enzyme. 19665938919
a clinical trial of cephaloridine.cephaloridine, a cephalosporin derivative, was administered to 30 selected patients, including 19 with moderate to severe impairment of renal function. this antibiotic eradicated infections due to staphylococcus pyogenes, and urinary tract infections due to a single member of the species escherichia coli or aerobacter aerogenes, which were sensitive to the drug on bacteriological testing. the drug failed in mixed urinary tract infections.no adverse effects were observed except for the developmen ...19665940638
[contribution to the study of the pyruvate-reductase of aerobacter aerogenes]. 19665942655
the structure of the aerobacter aerogenes a3(s1) polysaccharide. i. a reexamination using improved procedures for methylation analysis. 19665961274
the structure of the aerobacter aerogenes a3(s1) polysaccharide. ii. sequence analysis and hydrolysis studies. 19665961866
the properties of glycerol dehydratase isolated from aerobacter aerogenes, and the properties of the apoenzyme subunits. 19665962440
pneumohepatitis caused by klebsiella aerogenes. 19665963343
variation in content and distribution of magnesium, and its influence on survival, in aerobacter aerogenes grown in a chemostat. 19665969503
the adaptation of aerobacter aerogenes to the stress of sublethal doses of formaldehyde. 19665969748
role of lipid in the protection of staphylococcus aureus against trichlorophenol in mixed culture.the sensitivities of proteus mirabilis, salmonella schottmuelleri, aerobacter aerogenes, and staphylococcus aureus to 2, 4, 6-trichlorophenol in sodium borate were studied. it was demonstrated that these gram-negative organisms can protect s. aureus from the effect of the phenol in mixed culture. there is a direct correlation between this protective effect and the quantity of total lipid extracted from the gram-negative organisms. the distribution coefficient between trichlorophenol and the lipi ...19665970464
[a case of septicemia caused by aerobacter aerogenes]. 19665977185
magnesium starvation of aerobacter aerogenes. i. changes in nucleic acid composition.aerobacter aerogenes incubated in a medium containing all factors necessary for exponential growth except mg(++) continued to synthesize nucleic acids and proteins for more than 70 hr, provided the major carbon source was in excess at all times. after 24 hr of mg(++) starvation, deoxyribonucleic acid content in the culture had increased 10-fold. in contrast, the viable-cell count increased only about threefold during the first few hours and then remained approximately constant for the subsequent ...19676020411
magnesium starvation of aerobacter aerogenes. 3. protein metabolism.the metabolism of the ribosomal and soluble protein components of aerobacter aerogenes was examined during its incubation in a mg(++)-deficient medium. bacteria were exposed to leucine-h(3) during the exponential growth period preceding mg(++) starvation, and extracts were prepared after intervals of starvation and were centrifuged through gradients of sucrose to separate ribosomal from soluble proteins. ribosomal proteins synthesized during the preceding exponential growth were slowly lost from ...19676020412
magnesium starvation of aerobacter aerogenes. ii. rates of nucleic acid synthesis and methods for their measurement.the rates of synthesis of aerobacter aerogenes nucleic acids were estimated during incubation of the bacteria in a mg(++)-free medium. deoxyribonucleic acid (dna) synthesized during mg(++) starvation, or in the preceding exponential growth, remained acid-precipitable for 2.5 hr before breaking down to acid-soluble products during a period of many hours. rates of dna synthesis were calculated by correcting the net amounts of dna per milliliter to values that would have appeared had there been no ...19676021070
isolation of crystalline ph 6 acetolactate-forming enzyme from aerobacter aerogenes. 19676024768
metabolic injury to bacteria. ii. metabolic injury induced by distilled water or cu++ in the plating diluent.when distilled water from a tin-lined still served as the plating diluent, cells of aerobacter aerogenes developed symptoms of metabolic injury as evidenced by increased counts on supplemented, as compared with minimal, plating medium. cysteine was as effective as yeast extract as a supplement to the minimal medium in increasing the viable count. mg(++) and, to a lesser extent, phosphate buffer at the concentrations tested protected unfrozen cells, but not cells which had been frozen and stored, ...19676025433
influence of growing aerobacter aerogenes in a polyamine-enriched medium on the stability of the 70s ribosomes. 19676028139
the influence of dissolved oxygen concentration on the respiration and glucose metabolism of klebsiella aerogenes during growth. 19676029730
kinetic aspects of the growth of klebsiella aerogenes with some benzenoid carbon sources. 19676029731
potassium-ammonium antagonism in polysaccharide synthesis by aerobacter aerogenes nctc 418. 19676032041
effects of nitrogen supplements on nitrogen fixation by aerobacter aerogenes. 19676032045
inhibition of shigella flexneri by the normal intestinal flora. i. mechanisms of inhibition by klebsiella.growth curves were plotted for shigella flexneri and klebsiella (aerobacter aerogenes) multiplying in pure and mixed culture. in mixed culture, klebsiella inhibited shigella. exponential growth was interrupted and shigella entered into a logarithmic death phase. an analysis of cultures at the time inhibition occurred revealed that formic and acetic acids produced by klebsiella were responsible for the inhibition of shigella. klebsiella strongly reduced the culture medium. the volatile fatty acid ...19676032512
acyl phosphate: hexose phosphotransferase. purification and properties of the enzyme from aerobacter aerogenes and evidence for its common identity with hexose phosphate: hexose phosphotransferase. 19676033450
bactericidal activity for aerobacter aerogenes of bovine serum and cell-free normal and mastitic milks. 19676033692
phagocytosis and destruction of aerobacter aerogenes by leukocytes from bovine milk. 19676033693
further evidence for two distinct acetolactate synthetases in aerobacter aerogenes. 19676034688
dechlorination of 1,1,1-trichloro-2,2-bis(p-chlorophenyl)ethane by aerobacter aerogenes. i. metabolic products.whole cells or cell-free extracts of aerobacter aerogenes catalyze the degradation of 1,1,1-trichloro-2,2-bis(p-chlorophenyl)ethane (ddt) in vitro to at least seven metabolites: 1,1-dichloro-2,2-bis(p-chlorophenyl)ethylene (dde); 1,1-dichloro-2,2-bis(p-chlorophenyl)ethane (ddd); 1-chloro-2,2-bis(p-chlorophenyl)ethylene (ddmu); 1-chloro-2,2-bis(p-chlorophenyl)ethane (ddms); unsym-bis(p-chlorophenyl)ethylene (ddnu); 2,2-bis(p-chlorophenyl)acetate (dda); and 4,4'-dichlorobenzophenone (dbp). the use ...19676035049
phage-induced fucosidases hydrolysing the exopolysaccharide of klebsiella arogenes type 54 [a3(s1)].several strains of bacteriophage have been isolated that induce the formation of a polysaccharide hydrolase after infection of klebsiella aerogenes type 54 [a3(s1)]. the action of this enzyme on polysaccharide solutions was to decrease their viscosity and increase their reducing value. these effects were associated with the release of two oligosaccharides (o1 and o2) from the polysaccharide. these two substances are not identical with any of the four oligosaccharides isolated from autohydrolysat ...19676035518
enzymatic determination of vitamin b12, coenzyme b12, and other cobamide derivatives in picomole quantities by means of glycerol dehydratase from aerobacter aerogenes. 19676044270
the production of an n-acylanthranilic acid from shikimic acid and the effect on iron deficiency on the biosynthesis of other aromatic compounds by aerobacter aerogenes. 19676051584
gentamicin sulfate in the treatment of skin infection due to aerobacter aerogenes. 19676063497
the distribution of aerobacter aerogenes in the urinary tract of guinea pigs following ascending routes of inoculation. 19676068995
effects of humoral and cellular phases of acute inflammation in the bovine mammary gland on aerobacter aerogenes introduced en masse and on leukocytes existing in milk. 19676069569
the role of primer in glycogen biosynthesis in aerobacter aerogenes. 19676076622
[aerobacter aerogenes geeral infections in surgery]. 19675187116
multiple drug resistance in aerobacter aerogenes. 19675299909
two classes of pleiotropic mutants of aerobacter aerogenes lacking components of a phosphoenolpyruvate-dependent phosphotransferase system. 19675231354
influence of egg albumin-induced leukopenia on experimental aerobacter aerogenes mastitis and on natural infection of mammary gland with coagulase-negative staphylococcus in a cow. 19674964095
oxalacetate decarboxylase of aerobacter aerogenes. i. inhibition by avidin and requirement for sodium ion. 19674965256
the biosynthesis of tyrosine in aerobacter aerogenes: partial purification of the t protein. 19674965258
studies on the interaction of homologues of spermine with deoxyribonucleic acid and with bacterial protoplasts.four homologues of the naturally occurring polyamine spermine, of the type h(2)n.[ch(2)](3).nh.[ch(2)] (n).nh.[ch(2)](3).nh(2) where n=2, 3, 5 and 6, have been synthesized. their ability to stabilize escherichia coli protoplasts against osmotic lysis was compared with that of spermine. all homologues were approximately as effective as spermine. the effect of low concentrations of the homologues on the t(m) of calf thymus dna and of aerobacter aerogenes dna in 0.03m-sodium chloride-1mm-potassium ...19674860546
the reversal of phenylarsenoxide inhibition of keto acid oxidation in mitochondrial and bacterial suspensions by lipoic acid and other disulphides.1. inhibition of pyruvate oxidation in suspensions of aerobacter aerogenes cells and of isolated mitochondria from rat heart and liver by phenylarsenoxide is prevented by an excess of lipoic acid, whereas inhibition due to certain bivalent cations is not. 2. in both systems inhibition persists when the bacteria and mitochondria are recovered and resuspended in fresh media in the absence of the inhibitor. persistent inhibition due to preincubation with phenylarsenoxide, but not with the metal ion ...19674860547
ribosyl and deoxyribosyl transfer by bacterial enzyme systems.the enzymatic transfer of ribose and deoxyribose residues in pyrimidine nucleosides to purines was catalyzed by cell-free extracts of various bacteria. almost all the strains belonging to enterobacteriaceae were capable of catalyzing the transfer reactions. the transfer activities were also detected among some bacterial strains of other families: pseudomonadaceae, corynebacteriaceae, micrococcaceae, bacteriaceae, and bacillaceae. the rates of the transfer reactions were greatly enhanced in the p ...19674863982
the cell size and macromolecular composition of aerobacter aerogenes in various systems of continuous culture. 19674864933
the action of drugs on aerobacter aerogenes in various systems of continuous culture. 19674864934
production of isoamylase by escherichia intermedia.a culture of a coliform bacteria isolated from soil produced isoamylase when grown in peptone-maltose-salts broth. the enzyme preparation was partially purified. its similarity with isoamylase produced by aerobacter aerogenes was established by studying its action upon various polysaccharides. a study of the organism producing isoamylase showed that it differed from a. aerogenes in some of its physiological properties. this organism was identified as escherichia intermedia from morphological and ...19675340651
a ph 7 buffer devoid of nitrogen, sulfur, and phosphorus for use in bacteriological systems.apparent pk values were determined for a series of commercially available carboxylic acids in a search for a buffer suitable for bacterial studies at ph 7 in media free from nitrogen, sulfur, and phosphorus. of the compounds possessing pk' values near ph 7,3,6-endomethylene-1,2,3,6-tetrahydrophthalic acid (emta) was chosen as possessing suitable physical properties, cost, and purity for biological use. aerobacter aerogenes and escherichia coli grew normally in its presence but did not utilize it ...19675341860
nitrate reduction in aerobacter aerogenes. ii. characterization of mutants blocked in the reduction of nitrate and chlorate. 19675589517
[clinical picture and pathology of aerobacter aerogenes infection]. 19675591263
nitrate reduction in aerobacter aerogenes. 3. nitrate reduction, chlorate resistance and formate metabolism in mutant strains. 19675600617
[infections with aerobacter aerogenes. pathologic anatomical studies on the problem of hospitalism]. 19675631724
replacement of a phosphoenolpyruvate-dependent phosphotransferase by a nicotinamide adenine dinucleotide-linked dehydrogenase for the utilization of mannitol.mannitol is dissimilated by aerobacter aerogenes via an inducible pathway initiated by a phosphotransferase system dependent upon phosphoenolpyruvate as the phosphoryl donor. a mutational block in this pathway can be suppressed either at the phenotypic level by induction of d-arabitol dehydrogenase, an enzyme fortuitously capable of converting mannitol to fructose, or genotypically by a constitutive mutation in the d-arabitol system.19674289962
the hydrolysis of inositol phosphates by aerobacter aerogenes. 19674291648
isolation and properties of mutants of aerobacter aerogenes blocked in nitrate assimilation. 19674291666
the structure of the serotype 2 capsular polysaccharide of aerobacter aerogenes. 19674293395
derepression of phosphomonoesterase and phosphodiesterase activities in aerobacter aerogenes. 19674293966
nitrate reduction in aerobacter aerogenes. i. isolation and properties of mutant strains blocked in nitrate assimilation and resistant against chlorate. 19674297893
synthesis of rna, dna & protein during the growth of normal, viomycin-trained & reverted cultures of aerobacter aerogenes. 19674228208
[substrates and competitive inhibitor of assimilatory and dissimilatory nitrate reductase of aerobacter aerogenes]. 19674302524
magnesium starvation of aerobacter aerogenes. iv. cytochemical changes.a number of cytochemical changes were revealed by microscopic observations of aerobacter aerogenes populations starving for mg(++). during the first few hours, while the synthesis of deoxyribonucleic acid (dna) was paralleled by an increase in viable bacteria, the cells became progressively smaller. subsequently, the number of viable cells in the culture remained constant in spite of continuing dna synthesis, and the cells progressively elongated into filamentous forms. during this time, a secon ...19674164036
biodegradation of dichlorodiphenyltrichloroethane: intermediates in dichlorodiphenylacetic acid metabolism by aerobacter aerogenes. 196716349773
isolation of pectinolytic strains of aerobacter aerogenes. 196716349784
the action of barbitone on aerobacter aerogenes (klebsiella aerogenes). 196719108244
evidence for induction of the 2,3-butanediol-forming enzymes in aerobacter aerogenes. 196811946262
wild type variants of penicillinase from klebsiella aerogenes. 196811945261
[study of glucose metabolism in denitrifying bacteria. i. aerobacter aerogenes]. 19684234437
metabolism of d-fructose in aerobacter aerogenes: analysis of mutants lacking d-fructose 6-phosphate kinase and d-fructose 1,6-diphosphatase.the relative significance of the pathways for the conversion of d-fructose to d-fructose 1,6-diphosphate via d-fructose 1-phosphate or d-fructose 6-phosphate in aerobacter aerogenes prl-r3 was assessed by observing growth patterns of mutants lacking either d-fructose 6-phosphate kinase or d-fructose 1,6-diphosphatase. the mutant lacking d-fructose 6-phosphate kinase grew well on d-fructose or glycerol but not on d-glucose, whereas the mutant lacking d-fructose 1,6-diphosphatase grew on d-glucose ...19684298731
the action of ampicillin on aerobacter (klebsiella) aerogenes. 19684385438
uridine diphosphate d-glucose dehydrogenase of aerobacter aerogenes.uridine diphosphate d-glucose dehydrogenase (ec 1.1.1.22) from aerobacter aerogenes has been partially purified and its properties have been investigated. the molecular weight of the enzyme is between 70,000 and 100,000. uridine diphosphate d-glucose is a substrate; the diphosphoglucose derivatives of adenosine, cytidine, guanosine, and thymidine are not substrates. nicotinamide adenine dinucleotide (nad), but not nicotinamide adenine dinucleotide phosphate, is active as hydrogen acceptor. the p ...19684387161
pyruvate metabolism by aminopterin-inhibited aerobacter aerogenes.1. the synthesis and utilization of both alanine (by reductive amination, oxidative deamination and transamination) and valine (by transamination only) in aerobacter aerogenes are unaffected by aminopterin. these amino acids, which accumulate in aminopterin-treated cultures of this organism, are therefore considered to be formed as secondary products from the excess of pyruvate that also accumulates. 2. oxidative metabolism of pyruvate and the synthesis of acetylmethylcarbinol by a. aerogenes ce ...19685637348
pyruvate accumulation in growth-inhibited cultures of aerobacter aerogenes.1. accumulation of pyruvate occurs during the early stages of exponential growth of aerobic, anaerobic and static cultures of a strain of aerobacter aerogenes. in normal cultures of this organism the content of pyruvate increases until most of the glucose of the medium has been consumed, and then declines rapidly. the presence of unconsumed sugar is not the sole reason for the accumulation of keto acid, since this is unaffected by the addition of extra glucose to either exponentialphase or stati ...19685637349
the adaptation of klebsiella aerogenes to the inhibitory action of triethylene-melamine on growth and division. 19685637838
regulation of pentitol metabolism by aerobacter aerogenes. i. coordinate control of ribitol dehydrogenase and d-ribulokinase activities.induction studies on aerobacter aerogenes strain prl-r3, using ribitol as the inducer-substrate, indicated that two enzymes of ribitol catabolism, ribitol dehydrogenase and d-ribulokinase, are coordinately induced. the utilization of d-arabinose as a substrate resulted in the induction of ribitol dehydrogenase as well as d-ribulokinase. mutants which were constitutive for ribitol dehydrogenase were also constitutive for d-ribulokinase. in contrast, d-xylulokinase and d-arabitol dehydrogenase did ...19685643065
regulation of pentitol metabolism by aerobacter aerogenes. ii. induction of the ribitol pathway.the incubation of aerobacter aerogenes prl-r3 with ribitol resulted in the induction of ribitol dehydrogenase and d-ribulokinase, coordinately controlled enzymes of the pathway of ribitol catabolism. a dehydrogenase-negative mutant was unable to induce d-ribulokinase activity following incubation with ribitol. similar experiments using a kinase-negative mutant resulted in normal induction of ribitol dehydrogenase, as compared to the wild-type prl-r3 strain. constitutive or induced cells for l-fu ...19685643066
isolation and identification of n-acetylanthracnilic acid from quinic acid metabolism by aerobacter aerogenes. 19685645747
partial hydrolysis of dieldrin by aerobacter aerogenes. 19685647526
growth and bacteriolytic activity of a soil amoeba, hartmannella glebae.a soil amoeba, hartmannella glebae, could grow on a variety of gram-positive and gram-negative bacteria, although the rate of growth was faster in the presence of gram-negative bacteria. the amoeba, however, could not use yeasts, molds, or a green alga as a nutritional source. the extract prepared from amoebae grown in the presence of aerobacter aerogenes and alcaligenes faecalis could lyse intact cells and cell walls of many gram-positive bacteria at different rates. the spectrum of lytic activ ...19685651324
inhibition of glycerol dehydrogenase from aerobacter aerogenes by dihydroxyacetone, high ionic strength, and monovalent cations. 19685657454
the biosynthesis of tyrosine in aerobacter aerogenes. evidence for a suunit structure of the protein converting chorismate into 4-hydroxyphenylpyruvate. 19685658122
the ph 6 acetolactate-forming enzyme from aerobacter aerogenes. ii. evidence that it is not a flavoprotein. 19685658549
the ph 6 acetolactate-forming enzyme from aerobacter aerogenes. i. kinetic studies. 19685661161
the lipopolysaccharides of aerobacter aerogenes strains a3(s1) and nctc 243. 19685666760
mutants of aerobacter aerogenes capable of utilizing xylitol as a novel carbon.wild-type aerobacter aerogenes 1033 is unable to utilize xylitol. a succession of mutants was isolated capable of growth on this compound (0.2%) at progressively faster rates. whereas the ability to utilize xylitol was achieved in the first-stage mutant (x1) by constitutive production of ribitol dehydrogenase (for which xylitol is a substrate but not an inducer), the basis for enhanced utilization of xylitol in the second-stage mutant (x2) was an alteration of ribitol dehydrogenase. this enzyme ...19685674056
[aerobacter aerogenes as the cause of lethal sepsis in a small baby]. 19685675545
ionic relations as a function of metabolism in aerobacter aerogenes. 19685680993
experimental mastitis in leukopenic cows: immunologically induced neutropenia and response to intramammary inoculation of aerobacter aerogenes. 19685685004
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