Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| chromosomal mapping of brucella abortus, strain 19. | 1973 | 4631045 | |
| brucella melitensis rev. 1 and brucella abortus 45-20 vaccines in goats: serologic tests. | 1973 | 4631096 | |
| brucella melitensis rev. 1 and brucella abortus 45-20 vaccines in goats: pattern of immunoglobulin production after vaccination and challenge. | 1973 | 4631097 | |
| phenol-water fractions from smooth brucella abortus and brucella melitensis: immunochemical analysis and biologic behavior. | 1973 | 4632040 | |
| evaluation of an immunodiffusion test for the detection of antibodies to brucella abortus in bovine serum. | 1973 | 4632254 | |
| protection of rabbits against local vaccinia virus infection by brucella abortus and polyacrylic acid in the absence of systemic interferon production. | brucella abortus and polyacrylic acid, two agents whose interferon-inducing capacity in the mouse has been well established, failed to stimulate circulating interferon production in rabbits, yet protected rabbits against local vaccinia virus infection. | 1973 | 4355140 |
| a case of orchitis caused by brucella abortus in the african buffalo. | 1973 | 4716213 | |
| [effect of brucella abortus infection on the development of rauscher and l1210 leukemias in mice]. | 1973 | 4789989 | |
| growth characteristics of microorganisms occurring in penicillin-treated brucella abortus cultures. | 1973 | 4632484 | |
| migration inhibitory factor and interferon in the circulation of mice with delayed hypersensitivity. | when mice infected with mycobacterium tuberculosis strain bcg were inoculated intravenously with old tuberculin (ot) or living bcg cells, both migration inhibitory factor (mif) and interferon appeared in the circulation within a few hours. in such animals, which showed delayed hypersensitivity by footpad tests, as little as 1.5 mg of ot or as few as 1.7 x 10(6) bacteria per mouse were capable of eliciting circulating mif and interferon. uninfected animals inoculated with large doses of ot or liv ... | 1973 | 16558073 |
| the development of a new selective medium for the isolation of brucella abortus from contaminated sources. | 1974 | 4369280 | |
| antiviral activity of an ether-extracted nonviable preparation of brucella abortus. | extraction of living brucella abortus (strain 456) with aqueous ether yielded a nonviable, insoluble residue (bru-pel). when injected into mice, bru-pel was an effective, nontoxic interferon stimulus. mice pretreated with bru-pel were protected against challenge with otherwise lethal doses of semliki forest virus. significant protection was afforded when bru-pel was given as many as 7 days before virus challenge. evidence is presented dealing with the complex nature of bru-pel and with the possi ... | 1974 | 4373393 |
| [antigenic competition. response to a second antigen (sheep erythrocytes) in an animal persistently immunized with brucella abortus]. | 1974 | 4213980 | |
| the incidence of brucella infections in producer-retailer herds in north lancashire from 1965 to 1972. | the results are presented of testing untreated producer-retailer herd milk samples for the presence of brucella abortus during the period 1965-1972 in the north lancashire region.there was a steady decline in the incidence of infected herds from 22% in 1965 to 12% in 1971. a sharp fall to 5% in 1972 suggests that the introduction of the brucellosis incentives scheme and the eradication programme has helped to reduce the practice of selling brucella-infected cattle in the open market which was pr ... | 1974 | 4213998 |
| brucella abortus biotype 3 in tanzania. | 1974 | 4215211 | |
| genetic (h-2) dependence of the duration of splenomegaly induced by brucella abortus. | 1974 | 4215683 | |
| milk hygiene and bacteriology in the sudan: isolation of brucella abortus from cow's milk. | 1974 | 4220143 | |
| effect of brucella abortus infection (vaccine strain 19ba) on rauscher leukemia virus and l1210 leukemia in mice. | 1974 | 4208714 | |
| studies on a smooth phage-resistant variant of brucella abortus. i. immunological properties. | 1974 | 4209104 | |
| interferon induction in mice by lipopolysaccharide from brucella abortus. | endotoxin or lipopolysaccharide isolated from brucella abortus (strain 456) produced a typical endotoxin-type interferon response in mice with peak levels of the inhibitor occurring 2 h after intravenous injection of the stimuli. brucella lipopolysaccharide was a much less effective interferon stimulus than the lipopolysaccharide isolated from salmonella typhimurium (strain lt-2). | 1974 | 4210341 |
| effect of aflatoxin on serum proteins, complement activity, and the antibody response to brucella abortus in guinea pigs. | 1974 | 4211951 | |
| [virulence of brucella abortus species l forms for guinea pigs]. | 1974 | 4212877 | |
| serological cross-reactions between brucella abortus and yersinia enterocolitica ix. | 1974 | 4141713 | |
| incomplete restoration of the bursa-dependent immune system after transplantation of allogeneic stem cells into immunodeficient chicks. | transplantation of allogeneic cells from bursa of fabricius into cyclophosphamide-treated, immunodeficient chicks resulted in immunological tolerance to donor line skin grafts; graft-versus-host disease did not occur. allogeneic bursal stem cells taken from 3-day-old donors induced restoration of bursal morphology, of antibody formation to brucella abortus and of occurrence of pyroninophilic cells and immunoglobulin-bearing cells in the peripheral lymphoid tissues. secondary response to sheep re ... | 1974 | 4150769 |
| glucose transport in brucella abortus. | brucella abortus british strain 19 transported glucose with an apparent k(m) of 0.16 mm and an apparent v(max) of 250 nmol per min per mg of n. the only common glucose analogue transported was 2-deoxyglucose (2-dog), with an apparent k(i) of 0.73 mm. alpha- or beta-methyl glucosides and 3-o-methylglucose were not transported. transport was linear for 70 to 90 s, depending on the concentration of substrate used. 2-deoxyglucose was transported as the free sugar and was not further metabolized once ... | 1974 | 4206873 |
| occurrence of quinovosamine in lipopolysaccharides of brucella species. | lipopolysaccharides obtained from brucella abortus, b. melitensis, and b. suis, but not b. canis, were found to contain amino sugars identified as glucosamine and quinovosamine by cation exchange and thin-layer cellulose chromatography and ninhydrin degradation. | 1974 | 4207190 |
| isolation of substances responsible for lymphokine activity from sensitized mouse spleen cells. | cultures of brucella-sensitized mouse spleen cells exposed to brucella abortus antigens in vitro release macrophage migration inhibition factor (mif) and macrophage spreading factor. subjecting the supernatants from such cultures to preparative scale electrophoresis in acrylamide gel yields several fractions, one of which contains both mif and macrophage spreading factor. this material has properties attributable to guinea pig mif: it is nondialyzable, heat stable, nontoxic to macrophages from h ... | 1974 | 4208528 |
| biochemical basis of the serological cross-reactions between brucella abortus and yersinia enterocolitica serotype o:9. | a method based on the inhibition of agglutination is described that may be used for the differential serological diagnosis between b. abortus and y. enterocolitica serotype o:9. an antigen with high immunological capacity was isolated from brucella. this antigen inhibited both homologous and heterologous agglutination by brucella antiserum, but only the heterologous agglutination by yersinia antiserum. it proved to be constitued of a polysaccharide (n-acetylglucosamine, glucose, mannose and 2-ke ... | 1975 | 59566 |
| effect of ochratoxin and aflatoxin on serum proteins, complement activity, and antibody production to brucella abortus in guinea pigs. | the effect of ochratoxin alone and in combination with aflatoxin and brucella abortus antigen on complement activity, serum proteins, and antibody response in guinea pigs was investigated. ochratoxin did not affect complement activity or antibody response and there was no interaction between ochratoxin and aflatoxin on any of the responses tested. ochratoxin significantly lowered the level of beta-globulin in serum of guinea pigs. there was no significant interaction between aflatoxin and antige ... | 1975 | 45955 |
| erythritol catabolism by brucella abortus. | cell extracts of brucella abortus (british 19) catabolized erythritol through a series of phosphorylated intermediates to dihydroxyacetonephosphate and co-2. cell extracts required adenosine 5'-triphosphate (atp), nicotinamide adenine dinucleotide (nad), mg2+, inorganic orthophosphate, and reduced glutathione for activity. the first reaction in the pathway was the phosphorylation of mesoerythritol with an atp-dependent kinase which formed d-erythritol 1-phosphate (d-erythro-tetritol 1-phosphate) ... | 1975 | 163226 |
| inhibition of growth by erythritol catabolism in brucella abortus. | the growth of brucella abortus (us-19) in a complex tryptose-yeast extract medium containing d-glucose is inhibited by 10 mm erythritol. the enzymes of the erythritol pathway, except for d-erythrulose 1-phosphate dehydrogenase (d-glycero-2-tetrulose 1-phosphate:nicotinamide adenine dinucleotide (nad+) 4-oxidoreductase) were detected in the soluble and membrane fractions of cell extracts. glucose catabolism by cell extracts was inhibited by erythritol, whereas, phosphorylated intermediates of the ... | 1975 | 170249 |
| characterization of the electron transport system in brucella abortus. | the electron transport system in brucella abortus has been characterized. spectral studies of membrane preparations have indicated the presence of cytochromes a + a3 (maxima at 612 nm), cytochrome b (maxima at 560, 530, and 428 nm), cytochrome c (maxima at 552 and 522 nm), cytochrome o (maxima of carbon monoxide complex at 418 nm), and flavoproteins (minimum at 582 and 450 nm). cytochromes a + a3 appeared only after cells had reached late log phase, possibly due to lowered oxygen tension in the ... | 1975 | 235507 |
| the effects of ascorbic acid depletion on the immune response of the guinea-pig to brucella abortus strain 19 vaccine. | 1975 | 805147 | |
| brucella abortus biotype 1 as a cause of abortion in a bitch. | 1975 | 806159 | |
| brucellosis and veterinary surgeons. | forty-six veterinary surgeons were given a full clinical examination, serological examinations with estimates of immunoglobulins, and supplementary haematological and radiological investigations. thirty-five complained of one or more symptoms, and eight had abnormal physical signs which might have been caused by infection with brucella abortus, but neither sereological tests results nor immunoglobulin estimates bore any special relation to the clinical features. the soundest way of assessing ill ... | 1975 | 806320 |
| brucellosis in childhood. | brucellosis has always been an unusual disease in children and, concomitant with the control of the disease in domestic animals, reports have become sparse. the pediatrician, therefore, may not be aware of the protean clinical manifestations of childhood brucellosis. in 1973, nine cases occurred during a three-month period in el paso, texas. all cases were marked by spiking fevers and lethargy of four days to four weeks in duration. tender hepatomegaly or splenomegaly was striking in seven patie ... | 1975 | 806883 |
| stimulation of igg antibody formation by sublethal irradiation during persistently repeated immunization with brucella abortas. | whole-body irradiation with 600 rads 8 or 10 days after the beginning of persistently repeated brucella abortus immunization in the rabbit produces little change in the response. by contrast, irradiation with the same dose 2, 4 or 6 days after the beginning of repeated immunization stimulates the igg response. this effect is explained by a repopulation of antigen-sensitive cells from precursor cells to a higher level than that previous to irradiation. in order to explain igg stimulation, three m ... | 1975 | 807518 |
| fluid and solid media for isolation of brucella abortus. | the use of a fluid-enrichment technique increased by 10-16% the recovery rate of brucella abortus from milks of herds positive to a milk ring test. three solid media for direct cultural techniques were also tested on the same milks. combinations of these techniques produced a recovery rate of ca. 88% from mrt-positive-herd milks using a mixture of the centrifuged deposit and cream layer as the inoculum as against a recovery rate of ca. 75% using the mrt cream layer as inoculum. b. abortus biotyp ... | 1975 | 807615 |
| activated macrophages in congenitally athymic "nude mice" and in lethally irradiate mice. | resistance to the facultative intracellular bacteria, brucella abortus and listeria monocytogenes, is principally the result of acquisition of enhanced antibacterial activity by host macrophages, probably in response to lymphokines released by t lymphocytes. however, the present paper describes a surprisingly high resistance on the part of both congenitally athymic "nude" mice and of lethally irradiated mice compared with normal controls. this enhanced bactericidal activity was evident 24 hr aft ... | 1975 | 807647 |
| brucellosis: a study of five calves from reactor dams. | five calves from brucella abortus reactor cows bought after they had been on infected farms for two to six weeks, were reared in isolation and examined serologically for approximately six months prior to cultural and biological examination post mertem. one calf which had been serologically negative to the rose-bengal, serum agglutination and complement fixation tests for the last eight weeks of its life yielded a culture of br abortus biotype 1. this serotype had also been isolated from its dam ... | 1975 | 808019 |
| the bursal origin of an immunocompetent cell for antibody formation in the chicken. | surgically bursectomized and irradiated chickens were given bursal, splenic, bone marrow or thymic cells taken from syngeneic donors, together with killed brucella abortus and salmonella pullorum. blood samples were taken from those chickens 7 days later, and the serum agglutinin titres were determined. the cells of any lymphoid organ taken from 28-day-old chickens were more effective in restoring antibody response than those from 18-day-old ones. the restorative capacities of the bursa and sple ... | 1975 | 808466 |
| [brucella isolated in france: identification and typing. i. -- brucella abortus (author's transl)]. | two hundred and eight strains of brucella isolated from cattle and 5 isolated from man in various areas of france, during a two-year period from 1972 to 1974, were examined by all methods recommended by the subcommittee on taxonomy of the genus brucella and were found to have the same characteristics that identify and define the species brucella abortus. of the 213 strains, 105 (49,29 p. 100) were biotype i, 103 strains (48,36 p. 100) biotype 3, three strains (1,41 p. 100) biotype 4 and 2 strain ... | 1975 | 808998 |
| [nitrofurantoin-test for the differentiation of bordetella bronchiseptica (author's transl)]. | bordetella bronchiseptica is primarily resistant against nitrofurantoin (mic greater than 200 mug/ml), and this feature can be used for the differentiation of the organism from other gram-negative coccobacteria. nitrofurantoin paper disks (300 mug) failed to affect the growth of 150 strains of b. bronchiseptica isolated from different animal hosts, but they produced marked inhibition zones in the cultures of the followingspecies: pasteurella multocida, pasteurella pneumotropica, pasteurella haem ... | 1975 | 809944 |
| differentiation of cross-reacting antibodies against brucella abortus and yersinia enterocolitica by electroimmuno assay. | 1975 | 810011 | |
| [development of spleenic infection in the mouse following intravenous inoculation of various species of brucella in smooth (s) or rough (r) phases]. | the bacterial population in the spleen of mice inoculated intravenously by brucella abortus 544 (s), brucella suis 1330 (s), brucella melitensis 115 (r) or brucella canis rm 666(r) is studied kinetically. like the s strains of brucella, the r strains are able to multiply in the mouse spleen. the consequences for the tests of vaccines against natural infections produced by s or r strains of brucella are discussed. | 1975 | 810263 |
| the inability of surgical bursectomy in the newly hatched period to affect antibody formation in the pigeon (columba livia). | surgical bursectomy was performed in newly hatched pigeons within 24 hours of hatching. the effect of bursectomy on the immune responsiveness was evaluated by studying antibody formation to sheep red blood cells (srbc) and brucella abortus organisms and by the presence of serum immunoglobulins after immunoelectrophoresis. surgically bursectomized pigeons responded as well to srbc and brucella as did the non-bursectomized controls. there was no statistically significant difference in the geometri ... | 1975 | 810416 |
| a mechanism for prozone formation in the complement fixation test for bovine brucellosis. | complement fixation (cf) by bovine igg1 or igm antibodies to brucella abortus was inhibited by specific non-complement-fixing antibodies of the igg2 subclass. this inhibition may account for the appearance of prozones in cf titrations of some antiserums, and for the occurrence of serums which are positive to the rose bengal test, but negative to the cf test. | 1975 | 811200 |
| stimulation of protective immunity to salmonella kauffmann-white group n serotypes by brucella abortus and yersinia enterocolitica antigens. | 1975 | 811321 | |
| [adjuvant activity of the peptidoglycan isolated from the bacterial wall of brucella abortus 99]. | cell walls were obtained from brucella abortus 99. a peptidoglucan was isolated from them. test animals were immunized (albino mice) with pure human gammaglobulin, and in a series of experiments the peptidoglucan was added to the antigen, while in another one it was injected 24 hours later. the titer of the separated serum was determined in terms of the amount of the complement-fixing antibodies present, using the microreaction of complement fixation. it was established that with the addition of ... | 1975 | 812253 |
| immunoglobulin class specificity of non-agglutinating antibody produced in cattle following brucella abortus 45/20 vaccination. | the immunoglobulin class specificity of non-agglutinating antibody produced following vaccination with br. abortus 45/20 vaccine was determined using the antiglobulin test. in previously unvaccinated cattle it was found that most of the non-agglutinating antibody was associated with the igg1 immunoglobulin class. in cattle sensitised to brucella antigens by prior vaccination with brucella abortus strain 19 the majority of antibody was also associated with the igg1 immunoglobulin class, but a sig ... | 1975 | 812466 |
| studies on a smooth phage resistant variant of brucella abortus ii. mechanism of phage resistance. | the properties of a smooth phage resistant variant of brucella abortus were studied in an attempt to determine the mechanism of phage resistance. this strain was fully capable of adsorbing phage but penetration, and hence replication, did not occur. no evidence of lysogeny or a phage carrier state could be obtained and gross chemical differences between the resistant strain and its phage susceptible parent were not detected. the phage resistant variant showed increased resistance to lysis from w ... | 1975 | 812543 |
| leukocyte migration inhibition by a specific antigen in human brucellosis. | leukocyte migration inhibition /lmi/ tests were carried out in 19 patients with brucella abortus bovis infection and in 18 healthy control subjects using killed brucella abortus bovis as antigen. in the brucellosis group a statistically significant lmi was observed, as compared to the members of the control group. using the same antigen, a fairly good correlation was found between the local skin reaction and the results of the lmi test in the subacute or chronic stage of the disease. however, th ... | 1975 | 812829 |
| [structural and immunochemical studies on the immunoglobulin of carp (cyprinus carpio l.). iii. valence and affinity of antidinitrophenyl-antibodies]. | carps were immunized with dnp conjugated to human serum albumin, bovine gamma globulin and brucella abortus. the antibodies were isolated by immunoadsorption. the intrinsic average association constant (ko) of the antibodies was determined by equilibrium dialysis and ammonium sulphate precipitation employing the monovalent hapten tritiated epsilon-dnp-l-lysine. the tetrameric carp igm possesses four measurable combining sites per molecule. the affinity (ko) of the isolated antibodies varied from ... | 1975 | 788423 |
| vaccination against bovine brucellosis with a low dose of strain 19 administered by the conjunctival route. i.--protection demonstrated in guinea pigs. | guinea pigs were vaccinated with different doses of strain brucella abortus b 19, subcutaneously (sc) or by the conjunctival route (c), once or a second time three months later. agglutination and complement fixation titers were determined on sera of guinea pigs taken at random at intervals from each vaccine group. protection afforded by the vaccination was determined by intramuscular injection of the challenge strain, b. abortus 544 (5 x 10(3) bacteria) three months after the last vaccination. f ... | 1975 | 821381 |
| common enterobacterial antigen in yersinia enterocolitica. | production of the common enterobacterial antigen (ca) by strains of yersinia enterocolitica (y.e.) serotypes 3 and 9 (winblad), by strains of 5 different y.e. serogroups (wauters) and various other bacteria was examined. antibody against ca was raised by immunization of rabbits with e. coli o 14. extract prepared from s. typhimurium was used for the sensitization of sheep erythrocytes with ca. absorption and haemagglutination inhibition experiments showed that ca could be detected in heat extrac ... | 1975 | 1098394 |
| changes in the blood picture of cattle and calves following vaccination with brucella abortus (strain 45/20) vaccine. | 1975 | 1166713 | |
| diverse effects of bursectomy on humoral immune responses in the chicken. | chickens bursectomized hormonally or surgically at various ages in embryonic to postembryonic lives were immunized with a mixture of antigens, and the antibodies produced titrated. immune responses to salmonella pullorum (sp), brucella abortus (ba). staphylococcus aureus (staph) and ovalbumin (oa) were more markedly suppressed by the bursal deprivation than those to heterogenous erythrocytes, viruses and such bacteria as pasteurella multocida (pm) and escherichia coli b. the immune response to b ... | 1975 | 1187514 |
| [possible relationship between bacterial surface structures, immune-adherence, chemotaxis and phagocytosis (author's transl)]. | in the present study we attempted to determine the relationship between bacterial surface structures, immune adherence, chemotaxis and phagocytosis. as shown in table 1 encapsulated bacteria (klebsiella ozanae, k. pneumoniae, pasteurella multocida, streptococcus pneumoniae), mycoplasma pneumoniae and m. fermentans had a much lower immune adherence-activity than non-encapsulated bacteria (bacillus cereus, brucella abortus, erysipelothrix insidiosa, escherichia coli, listeria monocytogenes, pseudo ... | 1976 | 1258564 |
| properties of some new brucella phage isolates; evidence for lysogeny within the genus. | a number of phages were isolated from brucella abortus strains showing atypical phage sensitivity patterns. the phages, designated the firenze group, were all similar in morphology, host range general properties. they were lytic for smooth brucella abortus, br. neotomae and br. suis, but not for br. melitensis strains. they were serologically distinct from phages of the tbilisi and m51-s708-weybridge groups and also differed from these in plaque morphology and physical and chemical stability. th ... | 1976 | 1261749 |
| resistance of brucella abortus infected mice to intravenous or intraperitoneal brucella reinfection. | the development of acquired resistance to brucella abortus was studied in mice infected intravenously with small numbers of live co2-dependent b. abortus 544 used as vaccinal strain. co2-independent b. abortus was used as challenge strain. the influence of several parameters in the development of acquired resistance was examined: vaccination--challenge interval, challenge route, dose of challenge, challenge-sacrifice interval. the behavior of the challenge strain in normal mice depended on the s ... | 1976 | 821385 |
| brucella abortus in the bitch. | 1976 | 824792 | |
| laboratory summary of brucella isolations and typing: 1975. | during 1975 (fy), isolation or typing procedures (or both) were performed on 2,799 specimens for brucellae. of the 517 brucellae isolated or typed from cattle, 447 (86.4%) were brucella abortus, biotype 1. | 1976 | 824985 |
| vaccination against bovine brucellosis with a low dose of strain 19 administered by the conjunctival route. | eighteen 25 month old cows were vaccinated once either with 10(6) to 10(10) living bacteria of brucella abortus b 19 by the conjunctival route, or with 2.5 to 5 x 10(10) formalin-killed bacteria of the same strain by subcutaneous injection. fifteen days post-vaccination, low but definite agglutination titers were present in sera of cattle receiving 10(10) living bacteria by the conjunctival route, whereas high titers were observed in the control group given subcutaneous injection of killed bacte ... | 1976 | 825014 |
| antibody response and reduction in bursa of fabricius and spleen weights of progeny of chickens fed pcbs. | in experiment 1, polychlorinated biphenyls (aroclors 1016, 1232, 1242, 1248, and 1254) were fed ad libitum to white leghorn chickens at dietary levels of 5, 10, and 20 p.p.m. for 8 weeks. hatchability of eggs from hens fed 10 and 20 p.p.m. of aroclors 1232, 1242, and 1248 was reduced. the weights of the spleen and bursa of fabricius of day-old chicks from hens fed 10 p.p.m. of aroclors 1242 and 1248 or 20 p.p.m. of aroclors 1232, 1242, 1248, and 1254 were less than those of control chicks. body ... | 1976 | 825848 |
| enzyme-linked immunosorbent assay (elisa) for titration of antibodies against brucella abortus and yersinia enterocolitica. | an enzyme linked immunosorbent assay (elisa) using phenolwater extracted lipopolysaccarides as antigen was used for detection and quantitation of antibodies against brucella and yersinia bacteria in rabbit antisera. elisa was found to be from ten to hundred-fold more sensitive than the commonly used tube agglutination assay (widal). in addition, both direct and inhibition assays using elisa revealed antigenic differences between brucella abortus and yersinia enterocolitica o-group v, previously ... | 1976 | 826112 |
| a leucocyte migration study on the cell-mediated immunity against adult human oral mucosa and streptococcal antigens in patients with recurrent aphthous stomatitis. | in vitro demonstration of cell-mediated immunity (cmi) against adult human oral mucosa antigen (ahom) and streptococcal antigens was accomplished by means of the leucocyte migration test (lmt) in patients with recurrent aphthous stomatitis (ras). if antigenic extracts of ahom, streptococcus sanguis strain 2a or streptococcus pyogenes strain m5 were used in the lmt experiments, the distribution of migration indices (mi) over patients with ras differed significantly from the distribution over the ... | 1976 | 826113 |
| isolation of brucella abortus from two dogs in contact with bovine brucellosis. | on a farm where several cattle were serologically positive for bovine brucellosis, three dogs were found to have titres greater than 400 i.u. to brucella abortus. the titres persisted until the dogs were killed over two months later. two male dogs were necropsied. b. abortus was isolated from the spleen of both dogs. while farm dogs are not thought to be a major reservoir of bovine brucellosis they may be considered as possible carriers in imfected herds and should be considered during the inves ... | 1976 | 826308 |
| radioimmunoassay for antibodies against brucella abortus: a new serological test for bovine brucellosis. | a radioimmunoassay (ria) has been developed to measure antibodies against brucella abortus in bovine serum and can be used in the diagnosis of bovine brucellosis. the ria measures the amount of specific antibody of the igg1 and igg2 subclasses but is insensitive to igm, a characteristic which may make it more suitable than the complement fixation test (cft) or the serum agglutination test for distinguishing infected animals from those which have been vaccinated with br. abortus strain 19. the r ... | 1976 | 826587 |
| sensitivity of brucella spp to tetracycline and its analogues. | the sensitivity to eight tetracyclines of 100 strains of brucellae, comprising strains of brucella abortus, br. melitensis, and br. suis, was determined. demethylchlortetracycline was the most effecitve against all the groups of brucellae, whereas oxytetracycline and chlortetracycline were the least effective. the mean mic value for demethylchlortetracycline, doxycycline, lymecycline, and tetracycline was less than or equal to 1 mug/ml. strains of br. abortus biotype 2 and br. suis were the most ... | 1976 | 827554 |
| [chemical and immunologic study of the bacterial wall of brucella abortus 99]. | the investigations were carried out through the cft serologic activity of some of the brucella abortus 99 cell components. it was found that only 0.6 per cent of the weight of the bacterial walls could be extracted by means of physiologic saline. the extracts proved to be serologically active and specific for these brucella organisms only. the wall of brucella abortus 99 and its peptidoglucan were shown to be rich in lysine, leucine, glitamic acid, and valine, and poor in methionine, histidine, ... | 1976 | 827848 |
| infection and serological responses in cattle given 45/20 vaccine and later challenged with brucella abortus. | one of 2 groups of 15 non-pregnant cows was vaccinated twice 6 weeks apart by the subcutaneous route with a killed adjuvant 45/20 vaccine. at the time of the first vaccination a bull was introduced for a period of 14 weeks. all animals were challenged with 1.5 x 10(7) brucella abortus organisms given into the conjunctival sac 18 weeks after the second vaccination of the vaccinated group. the largest lesions at the site of vaccination (8 cm diam.) were seen 2 weeks after inoculation. small lesion ... | 1976 | 828049 |
| relation between macrophage migration inhibition and immunity to brucella abortus in guinea-pigs. | relation between macrophage migration inhibition and immunity to brucella abortus in guinea-pigs. onderstepoort journal of veterinary research 43 (4), 175-184 (1976). a soluble protein antigen was prepared from brucella melitensis rev i with which macrophage migration inhibition (mmi) assays were successfully done using guinea-pig peritoneal exudate cells. by comparing the mmi, agglutinin response and immunity of groups and of individual guinea-pigs which had been immunized with either b. melite ... | 1976 | 828715 |
| [vaccinal strain of brucella abortus 19-ba in ticks (argasidae) infected by feeding]. | 1976 | 828951 | |
| the augmentation of antibody responses by preliminary intrabursal priming in the chicken. | direct injection of antigen into bursal tissue of young chickens followed by subsequent intravenous immunization markedly stimulated agglutinin production against brucella abortus. in contrast, preliminary intravenous immunization did not produce stimulation. the promoting effect of intrabursal injection was antigen-specific. antigen injection into the bursa reduced the extent to which subsequent bursectomy suppresses the immune response. bursa cells from young chickens which had been injected w ... | 1976 | 829231 |
| evaluation of five mediums for the stabilization of brucella abortus strain 19 dessicated by lyophilization. | lyophilized brucella abortus strain 19 vaccines were prepared using five recommended mediums. these five vaccines were compared with each other and with liquid vaccine relative to their suitability for (1) maintaning distinguishing cultural characteristics; (2) maintaining viability during lyophilization; (3) quality of restoration; (4) maintaining viability under various storage temperatures and (5) maintaining stable, antigenic and virulence qualities. | 1976 | 829491 |
| brucella abortus infection in sheep. ii. experimental infection of ewes. | 1976 | 963495 | |
| endotoxic activity of rough organisms of brucella species. | a rough-specific antigen extracted from the rough species brucella ovis and lipopolysaccharide extracted from smooth brucella abortus demonstrated equivalent levels of activity in tests for mouse lethality and limulus lysate clotting activity. acetone-extracted whole cells of b. ovis and of b. canis and of a rough mutant of b; abortus had the same toxicity for mice, but it was not possible to extract endotoxin from b. canis by the methods used. | 1976 | 971943 |
| vaccination against bovine brucellosis with a low dose of strain 19 administered by the conjunctival route. iii. -- serological response and immunity in the pregnant cow. | seventy-four heifers, 7 to 12 months old, were randomized in four groups: group a, 8 heifers as controls; group b, 19 heifers vaccinated subcutaneously with 9 x 10(10) brucella abortus strain b 19; group c, 19 heifers vaccinated as in group b, then revaccinated by the conjunctival route 6 to 8 months later with 5 x 10(9) bacteria; group d, 28 heifers vaccinated twice by the conjunctival route with the same dose and time intervals as in group c. serological responses in agglutination, complement ... | 1976 | 984715 |
| the effects of nutritional rehabilitation on antibody production in protein-deficient mice. | the transfer of chronically protein-deficient mice to an optimal diet a few days before immunization with sheep erythrocytes, tetanus toxoid or brucella abortus vaccine either failed to increase antibody production above the level produced by deficient mice, or suppressed the responses to below those produced by deficient mice or normally-fed controls. transfer to high protein diet on the day of immunization or feeding deficient mice the normal diet for just 2 days at the time of injection produ ... | 1976 | 992718 |
| immune mechanisms affecting bovine leucocytes during suppression of tuberculin skin sensitivity in cattle. | complete desensitization to tuberculin skin-testing (anergy) was produced in cattle by repeated intravenous injections of living bcg organisms into animals sensitized by a prior subcutaneous dose of bcg. two levels of desensitization were produced; complete desensitization following 10 i.v. doses and partial desensitization following 5 i.v. doses of 100 mg bcg. intradermal tuberculin testing at the end of the experiment stimulated the appearance of reactive blood lymphocytes in sensitized cattle ... | 1976 | 797673 |
| the immunogenic activity of ribosomal fractions derived from brucella abortus. | the immunizing activity of ribosome preparations derived from brucella abortus strain 19 cells was examined in guinea-pigs and mice. after subcutaneous injections of br. abortus ribosomes in freund's incomplete adjuvant, both mice and guinea-pigs developed immunity to challenge by virulent br. abortus 544 organisms which was at least as effective as the protection conferred by live strain 19 vaccine. both mice and guinea-pigs also developed agglutinating and complement-fixing antibodies and dela ... | 1976 | 812900 |
| humoral immune responses characteristic of testosterone-propionate-treated chickens. | white leghorn chickens treated with testosterone-propionate on the 3rd day of embryonation were immunized with a mixture of sheep red blood cells, brucella abortus and salmonella pullorum at various ages, and the resulting agglutinins were titrated. the production of igm antibody against sheep red blood cells was not affected significantly by testosterone-propionate. on the contrary, immune responses against the bacterial antigens were strongly suppressed by the same treatment. production of igg ... | 1976 | 815175 |
| identification of brucella abortus antibodies in cattle serum by single radial diffusion. | single radial diffusion combined with the rose bengal test permitted rapid identification of all of the brucella abortus-infected cattle in a study group of 689 animals. | 1976 | 815271 |
| evolution and taxonomy in the genus brucella: contemporary evolutionary status of the species brucella abortus. | the biotypes in the species brucella abortus were arranged according to the sequential flow in the alterations of their characteristics. this reordering of the biotypes indicated that the organism currently classified as b abortus type 2 is the parent organism of all the biotypes in this species. however, laboratory-selected mutants from b abortus type 2 had characteristics that were mimetic, but not duplicative, of their naturally occuring counterparts. evidence which indicates this phenomenon ... | 1976 | 816231 |
| evolution and taxonomy in the genus brucella: steroid hormone induction of filterable forms with altered characteristics after reversion. | several strains of brucella abortus, brucella suis, and brucella melitensis were exposed to physiologic concentrations of testosterone, progesterone, and diethylstilbestrol by incorporating them into the growth medium. the hormones induced brucella to form cell wall-defective organisms, including filterable forms. the filterable forms from 1 strain of b melitensis had altered characteristics when it reverted to growth as an intact cell. this is the 1st reported instance of induction of filterabl ... | 1976 | 816232 |
| evolution and taxonomy in the genus brucella: progesterone induction of filterable forms of brucella abortus type 2 with revertant characteristics essentially indistinguishable in vitro from those of brucella ovis. | an organism essentially indistinguishable from brucella ovis, as determined by conventional and manometric techniques of identifying brucella organisms, was derived in vitro from a strain of brucella abortus type 2. the basic mechanism responsible for derivation of this organism was incomplete reversion of the l-forms to their original parental characteristics. the agent used to induce the l-forms was a physiologic concentration of progesterone incorporated in the growth medium. | 1976 | 816233 |
| the in vivo activity of a smooth phage-resistant variant of brucella abortus strain 19. | 1976 | 816417 | |
| brucella abortus infection in sheep. i. field case. | 1976 | 816418 | |
| [biological properties and chemical composition of a soluble fraction of brucella abortus. monospecific a or m antigens]. | abs, the supernatant of smooth b. abortus phenol-protected suspensions, is a protein, lipid, sugar and arn complex containing all amino acids found in phenol-water fractions from b. abortus and b. melitensis. abs is non-toxic and immunizes mice against b. abortus challenge. chromium chloride easily binds abs to sheep erythrocytes (e abs) for a specific and accurate passive hemagglutination test in brucellosis. e abs are agglutinated by all antisera to fractions of b. abortus or b. melitensis, bu ... | 1976 | 816680 |
| the immunological properties of brucella ribosomal preparations. | ribosomes were isolated from brucella abortus strains 19 and 45/20 by disruption of the cells followed by differential ultracentrifugation. the ribosome preparations contained 2-3 components reacting in immunodiffusion tests but were free of detectable lipopolysaccharide-protein agglutinogen. they crossreacted with antisera to br. abortus, br. melitensis, br. suis and br. ovis and elicited intradermal delayed hypersensitivity reactions in animals infected with br. abortus, br. melitensis or br. ... | 1976 | 816681 |
| the immune response to brucella abortus 45/20 adjuvant vaccine in terms of immunoglobin class. | cattle vaccinated with brucella abortus 45/20 adjuvant vaccine developed agglutinins for rough brucella antigens which were largely of the igg classes. only small amounts of igm rough agglutinins were produced at the earliest stages of the serological response. non-agglutinating antibodies detectable in the antiglobulin test were mainly of igg2 and iggi classes. cattle previously exposed to smooth brucella antigens, after vaccination with 45/20 adjuvant vaccine, initially developed high titres o ... | 1976 | 816682 |
| [influence of the mouse genotype on the sensitivity to brucella abortus 544]. | the susceptibility to brucella infection (brucella abortus 544) of different inbred mice and outbred cd1 strains has been studied. the degree of infection was determined on days 7 and 11 after challenge. the cba and dba2 strains showed greater susceptibility and homogeneity compared to the other strains (c57b16, c3h, c57b16 x dba2) and to the cd1 mice. the kinetics of infection in dba2 and cba, compared to those of the cd1 for a longer period, show that the degree of infection decreases rapidly ... | 1976 | 816685 |
| [evaluation of antibrucellosis vaccines using guinea pigs]. | in the preliminary stage of a global study made in our laboratory to compare the control technics of antibrucella vaccines (abortus) both in mice and guinea pigs, we have studied the kinetics of brucella infection in the hartley guinea pig after various doses of brucella abortus strain 544. two trials have been carried out: the first with 5000 and 10000 bacteria and the second with 5000, 10000 and 30000 bacteria. the results obtained allow to envisage the use of a challenge dose of at least 1000 ... | 1976 | 816686 |
| an assessment of investigations conducted in the usa on brucella abortus strain 45/20 bacterins. | the results of five investigations in cattle using 45/20 bacterins are presented and evaluated. seemingly contradictory results may be the result of one or more modifications in project design or in procedures. in these trials, there appeared to be no significant difference in the level of protection for cattle when using either intramuscular or subcutaneous routes of injection or when 10 or 12 week intervals between doses were used in cattle 6 months of age or older. also, the relative resistan ... | 1976 | 816689 |
| a recent trial comparing two 45/20 adjuvant brucella vaccines. | two commercially prepared killed 45/20 adjuvant brucella abortus vaccines were compared, under controlled experimental conditions, with regard to local reactions at the site of injection, serological reactions and immunogenicity. preliminary trials were carried out in yearling cattle and guinea pigs. three groups of 13 maiden heifers were involved. one group was vaccinated with abortox, one group was vaccinated with duphavac and the third group was kept as unvaccinated controls. following servic ... | 1976 | 816690 |
| the serological response to rough and smooth brucella antigens in cattle vaccinated with brucella abortus strain 45/20 adjuvant vaccine. | cattle not previously exposed to brucella abortus responded to a single dose of br. abortus 45/20 adjuvant vaccine by producing low titres of agglutinins for rough brucella antigens and little or no agglutinins to the smooth antigens. moderate titres of non-agglutinating antibodies reacting with both rough and smooth antigens were also produced. after a second dose of 45/20 vaccine higher titres of rough agglutinins and low titres of antibodies reacting with smooth antigens were produced. high t ... | 1976 | 816691 |
| [brucella and modification of the host response to bacterial and viral infection. effect of the s-r variation]. | within the framework of a study on the immunostimulant properties of brucella abortus and of its extracts, we have investigated the protective activity on infection of mice by klebsiella pneumoniae and on charlotte friend's leukemia, of inactivated brucella abortus and brucella lysates by ultrasound. the injection of inactivated b 19 r preparations and their lysates three days before infection by klebsiella has allowed to protect mice against a large number of dl50 (100 and 1000 in certain cases ... | 1976 | 816692 |
| a complement fixation method for quantitative differentiation of reactions to 45/20 vaccine and brucella infection. | brucella complement fixing antibodies may be titrated independently in adult vaccinated (strain 45/20) and in naturally infected cattle by serological tests utilizing a specially prepared antigen. serum samples are subjected to the standard complement fixation test for the diagnosis of brucellosis (mackinnon 1963) and subsequently retested by the same method but with a saline extract antigen prepared from strain 45/20 brucella abortus. the results obtained in the two tests are compared in five s ... | 1976 | 817437 |
| brucella abortus infection in the bull. | observations on 2 bulls from a brucella-infected property are reported. bull 1 gave serological reactions to br. abortus in both the sat and cft from day 0 to day 141. br. abortus was not recovered from semen and the bull remained clinically normal. the serological status of bull 2 changed from negative to positive to negative over a 203 day period and remained negative for a further 74 days. semen agglutinins were only detected on one occasion (20 iu). the first clinical sign observed was epidi ... | 1976 | 817703 |
| antiviral activity of brucella abortus preparations; separation of active components. | injection into mice of heat-killed brucella abortus or aqueous ether-extracted b. abortus (bru-pel) induced a "virus-type" interferon response, with peak titers at 6.5 h. the animals also were protected against challenge with otherwise lethal doses of semliki forest virus. extraction of either heated b. abortus or bru-pel with a mixture of chloroform-methanol (2:1, vol/vol) (c-m( yielded an insoluble residue (extracted cells) and a c-m extract. neither extracted cells nor c-m extract alone induc ... | 1976 | 818014 |