Publications

characterization of recombinant murine interleukin 5 expressed in chinese hamster ovary cells.	we have purified recombinant murine interleukin 5 (rmil-5) from the supernatant of chinese hamster ovary cells. each peptide fragment of the purified rmil-5 generated by achromobacter protease i digestion was characterized and glycosylation sites were determined. although rmil-5 contains three potential sites of n-linked glycosylation (asn-26, asn-55 and asn-69), asn-69 is not glycosylated. the oligosaccharides released from the protein by hydrazinolysis were fractionated by paper electrophoresi ...	1992	1457971
vibrio alginolyticus ("achromobacter") collagenase: biosynthesis, function and application.	bacterial collagenase from aerobic non-pathogenic vibrio alginolyticus chemovar iophagus ("achromobacter" collagenase, ec 3.4.24.08) is an inducible extracellular metallo-proteinase. production of vibrio collagenase is induced specifically by collagen or by its macromolecular fragments. on the cell surface is expressed a specific receptor recognizing collagen structure. the study of natural inducers led to synthetic peptides with inducing properties. vibrio collagenase cleaves collagen helical c ...	1992	1480012
effect of achromobacter collagenase on fibronectin: activation of fn-gelatinase and fn-laminase.		1992	1480015
sequence determination and characterization of a phospholipase a2 isozyme from trimeresurus gramineus (green habu snake) venom.	in addition to phospholipase a2-i (pla2-i) reported previously (oda et al., 1991, toxicon 29, 157), a new pla2 named pla2-ii was isolated from trimeresurus gramineus (green habu snake) venom, and its amino acid sequence was determined by sequencing the native protein and the peptides produced by enzymatic (achromobacter protease i and clostripain) cleavages of the carboxamidomethylated derivative of the protein. the protein consisted of 122 amino acid residues and his-47, asp-48, and asp-98 whic ...	1992	1485333
bacterial arthritis.	the 1991 literature on septic arthritis included a concise review of adult septic arthritis, examples of pseudoseptic arthritis, and two interesting animal studies. one animal study examined the induction of acute synovitis by the intra-articular injection of bacterial endotoxin and the cytokines tumor necrosis factor-alpha, and interleukin-1 beta; and the other studied the effects of early and delayed synovectomy in the management of septic arthritis. the predispositions to septic arthritis can ...	1992	1503874
molecular cloning and characterization of catechol 2,3-dioxygenases from biphenyl/polychlorinated biphenyls-degrading bacteria.	catechol 2,3-dioxygenases were cloned from alcaligenes sp. kf711, pseudomonas putida kf715, and achromobacter xylosoxidans kf701 which are biphenyl/polychlorinated biphenyls-degrading bacteria. all of the cloned enzymes were purified by preparative polyacrylamide gel electrophoresis (page). the purified catechol 2,3-dioxygenases were significantly different from one another in ring-fission activities to catechol and its derivatives. the catechol 2,3-dioxygenase from alcaligenes sp. kf711 exhibit ...	1992	1530619
characterization of catechol 2,3-dioxygenases.	three catechol 2,3-dioxygenases for biphenyl, naphthalene/salicylate, and toluene/xylene oxidation were cloned from achromobacter xylosoxidans kf701, pseudomonas putida (nah7), and pseudomonas sp. (pwwo). the cloned catechol 2,3-dioxygenases were identified by enzymatic activity assay in addition to yellow bands on polyacrylamide gel after electrophoresis and activity staining. all of the cloned catechol 2,3-dioxygenases exhibited their highest activities on catechol as a substrate compared with ...	1992	1543511
bacteremia due to achromobacter xylosoxidans in patients with cancer.	bacteremia due to achromobacter xylosoxidans is rare, and little information on treatment is available. between 1983 and 1988, a. xylosoxidans was recovered from 26 cultures of blood from 10 patients with cancer and clinical signs of infection, including one patient with septic shock and two with pneumonia. neutropenia did not seem to be a predisposing factor. the infection may have been catheter related in four patients and associated with gastrointestinal pathology in four others. probable cau ...	1992	1554834
catheter-associated sepsis caused by ochrobactrum anthropi: report of a case and review of related nonfermentative bacteria.	ochrobactrum anthropi, formerly known as cdc group vd, is an oxidase-producing, gram-negative, non-lactose-fermenting bacillus that oxidizes glucose and grows readily on macconkey agar. only occasionally isolated from human clinical specimens, this organism has rarely been found to be pathogenic. we describe the first reported case of infection due to o. anthropi in a child, that of bacteremia in a 3-year-old girl undergoing chemotherapy for retinoblastoma. in addition, we review the literature ...	1992	1576286
evidence that the type 2 copper centers are the site of nitrite reduction by achromobacter cycloclastes nitrite reductase.	methods have been developed for selective depletion and reconstitution of the type 2 cu (non-blue) sites in the nitrite reductase from a. cycloclastes, resulting in preparations ranging from 0.5 to 2.6 type cu per trimer; the type 1 cu content is invariant at 3.0 per trimer. the activity of the enzyme is directly proportional to the type 2 content as measured by direct metal determination or by analysis of the epr spectra. these results indicate that an earlier report that the a. cycloclastes en ...	1992	1329738
cloning and expression of a chick liver glutathione s-transferase cl 3 subunit with the use of a baculovirus expression system.	glutathione s-transferase cl 3 subunits purified from 1-day-old-chick livers were digested with achromobacter proteinase i and the resulting fragments were isolated for amino acid sequence analysis. an oligonucleotide probe was constructed accordingly for cdna library screening. a cdna clone of 1342 bases, pgcl301, encoding a protein of 26209 da was isolated and sequenced. including conservative substitutions, this protein has 75-79% sequence similarity to other alpha family glutathione s-transf ...	1992	1339283
proteolytic processing of human lysosomal arylsulfatase a.	arylsulfatase a purified from human placenta contained an unreported component with an apparent molecular mass of 7 kda in addition to the two known components with apparent molecular masses of 58 and 50 kda. the detailed relationship between the 58 kda component and the 50 kda component is as yet unknown. the present study was undertaken to define the structure of the subunits of the sulfatase. the n-terminal sequence of the 50 kda component was identical to that of the 58 kda component. furthe ...	1992	1352993
evidence for the existence of isozymes of glucose isomerase from streptomyces phaeochromogenes.	glucose isomerase from streptomyces phaeochromogenes was purified from a commercial preparation, swetase, by deae-cellulose, bio-gel a-0.5 m, and hydroxyapatite column chromatographies. it was found to be 2 fractions; f-a, not adsorbed on hydroxyapatite and f-b, adsorbed on hydroxyapatite. they were homogeneous in ordinary and sds-page and had similarities in some enzymatic and physico-chemical properties. the differences, however, were found in the n-terminal amino acid, which was only serine f ...	1992	1368294
mouse gamma-casein cdna: pcr cloning and sequence analysis.	a partial amino acid sequence of mouse gamma-casein was determined on a gas-phase amino acid sequencer. the n-terminal sequence (23 residues) was obtained using native gamma-casein, and the c-terminal sequence (13 residues) was determined with a corresponding peptide. the c-terminal peptide was isolated by affinity chromatography on an anhydrotrypsin agarose column after digestion of gamma-casein with achromobacter lysyl-endopeptidase. a cdna (507 base pairs) encoding the mature protein of gamma ...	1993	7763793
isolation and characterization of an n-methylcarbamate insecticide-degrading methylotrophic bacterium.	a gram-negative bacterium which hydrolyzed aryl n-methylcarbamate insecticides was isolated from an agricultural soil which quickly degraded these pesticides. this organism, designated strain er2, grew on carbofuran as a sole source of carbon and nitrogen with a doubling time of 3 h in a mineral salts medium. the aromatic nucleus of the molecule was not metabolized, and carbofuran 7-phenol accumulated as the end product of metabolism. the insecticides carbaryl, bendiocarb, and propoxur were simi ...	1993	7504430
sweet potato beta-amylase. primary structure and identification of the active-site glutamyl residue.	the complete amino acid sequence of a subunit of sweet potato beta-amylase, a homotetramer, was established by sequence analysis of peptides obtained by digestions with achromobacter protease i and staphylococcus aureus v8 protease and by cyanogen bromide cleavage of the s-carboxymethylated subunit. the subunit of the enzyme is a single polypeptide consisting of 498 amino acid residues. it showed 50-60% identity in the amino acid sequence with those of beta-amylases from soybean and barley, whil ...	1993	8103452
crystallization and preliminary x-ray studies on pseudoazurin from achromobacter cycloclastes iam1013.	new crystals of a blue copper protein, pseudoazurin from denitrifier achromobacter cycloclastes iam1013, have been obtained by means of vapor diffusion with ammonium sulfate as a precipitant at ph 6.0 and 4 degrees c. the crystals belong to the orthorhombic system, space group p2(1)2(1)2(1), with unit cell dimensions of a = 56.69(2), b = 61.53(2), and c = 30.20(1) a. the asymmetric unit includes one molecule of pseudoazurin with a vm value of 2.04 a3/da. the crystals are so stable against x-ray ...	1993	8138527
purification, sequencing and characterization of single amino acid-substituted phospholipase a2 isozymes from trimeresurus gramineus (green habu snake) venom.	two phospholipases a2 named pla2-iii and iv were newly isolated from trimeresurus gramineus (green habu snake) venom in addition to pla2-i and ii reported previously [oda et al. (1991) toxicon 29, 157; fukagawa et al. (1992) toxicon 30, 133]. their isoelectric points were determined to be about 4.5. pla2-iii and iv exhibited almost unchanged lipolytic activity toward egg-yolk when compared with pla2-i. the amino acid sequences were determined by sequencing the native proteins and the peptides pr ...	1993	8212048
resonance raman spectroscopy of the azurin his117gly mutant. interconversion of type 1 and type 2 copper sites through exogenous ligands.	the copper center of the pseudomonas aeruginosa his117gly azurin mutant is accessible to exogenous ligands through an aperture in its surface created by the removal of the endogenous imidazole ligand. depending on the exogenous ligand, a surprising variety of type 1 and type 2 copper sites can be obtained that are readily distinguished by electronic, epr, and resonance raman (rr) spectroscopy. the rr spectrum of type 1 h117g with exogenous imidazole is nearly identical to that of wild-type azuri ...	1993	8241136
achromobacter xylosoxidans osteomyelitis.		1993	8280199
achromobacter xylosoxidans (alcaligenes xylosoxidans subspecies xylosoxidans) bacteremia after liver transplantation.		1993	8294634
ochrobactrum anthropi bacteremia: report of four cases and short review.	ochrobactrum anthropi, formerly "achromobacter" cdc group vd, is a nonfermentative, nonfastidious gram-negative bacillus, that only recently has been given attention as a potential human pathogen. over a 2-year period, we observed four patients with multiple blood cultures that were positive for the organism. the patients had acute leukemia as underlying disease, and presented with clinical and microbiologic features consistent with catheter-related bacteremia. in three of the patients the infec ...	1993	8300247
x-ray scattering using synchrotron radiation shows nitrite reductase from achromobacter xylosoxidans to be a trimer in solution.	we demonstrate here the applicability of x-ray scattering for studying molecular conformation of multimeric proteins in solution by using synchrotron radiation to extend the range of data collection to include medium angles (ca. 3-4 degrees). we have been able to define the solution structure of the dissimilatory nitrite reductase of achromobacter xylosoxidans (axnir), an enzyme for which there are conflicting reports as to the nature of its multimeric structure. quantitative interpretation of t ...	1993	8338833
copper-containing nitrite reductase from pseudomonas aureofaciens is functional in a mutationally cytochrome cd1-free background (nirs-) of pseudomonas stutzeri.	the structural gene, nirk, for the respiratory cu-containing nitrite reductase from denitrifying pseudomonas aureofaciens was isolated and sequenced. it encodes a polypeptide of 363 amino acids including a signal peptide of 24 amino acids for protein export. the sequence showed 63.8% positional identity with the amino acid sequence of "achromobacter cycloclastes" nitrite reductase. ligands for the blue, type i cu-binding site and for a putative type-ii site were identified. the nirk gene was tra ...	1993	8352648
[relationship between water pollution and bacterial flora in river water].	to clarify the relationship between water pollution and bacterial flora in rivers, 132 samples of river water were collected at eleven stations of the chikuma-sai river system from april 1985 to march 1986, and 29 biological-physicochemical examinations for indices of water pollution were done using these samples. species and genus of bacteria in 485 isolates from bacterial flora were identified. although variations of bacterial flora were seen among sampling stations and sampling seasons, most ...	1993	8377255
osteomyelitis due to achromobacter xylosoxidans.		1993	8399894
primary structure of two-chain botrocetin, a von willebrand factor modulator purified from the venom of bothrops jararaca.	the complete amino acid sequence and location of the disulfide bonds of two-chain botrocetin, which promotes platelet agglutination in the presence of von willebrand factor, from venom of the snake bothrops jararaca are presented. sequences of the alpha and beta subunits were determined by analysis of peptides generated by digestion of the s-pyridylethylated protein with achromobacter protease i or alpha-chymotrypsin and by chemical cleavage with cyanogen bromide or 2-(2'-nitrophenylsulfenyl)-3- ...	1993	8430107
characterization of the structural gene encoding a copper-containing nitrite reductase and homology of this gene to dna of other denitrifiers.	a copper-containing nitrite reductase gene (niru) from pseudomonas sp. strain g-179 was found in a 1.9-kb ecori-bamhi dna fragment. the coding region contained information for a polypeptide of 379 amino acids. the encoded protein had 78% identity in amino acid sequence to the nitrite reductase purified from achromobacter cycloclastes. the ligands for type 1 copper- and type 2 copper-binding sites found in a. cycloclastes were also found in pseudomonas sp. strain g-179, suggesting that these bind ...	1993	8439151
cellular fatty acid compositions of "achromobacter groups b and e".	strains of "achromobacter groups b and e" were examined for cellular fatty acid (cfa) composition to evaluate their chemical relatedness to known bacterial species and groups. the cfas were liberated from whole cells by base hydrolysis, methylated, and analyzed by capillary gas-liquid chromatography. the cfa profiles of the two groups were identical and were distinct from cfa profiles of all other bacteria we have previously tested. these data provide support for results from whole-cell protein ...	1993	8463379
achromobacter xylosoxidans keratitis following penetrating keratoplasty.	achromobacter xylosoxidans is a bacterium not previously reported in a corneal infection. we present a case of infectious keratitis caused by this organism, occurring 1 month following penetrating keratoplasty.	1993	8481378
pro-major basic protein has three types of sugar chains at the pro-portion.	the amino-acid sequence of purified recombinant pro-major basic protein from chinese hamster kidney cells was determined to verify the primary structure and glycosylation sites. reduced and s-carboxamidemethylated protein was first digested with achromobacter proteinase i. each peptide was characterized by amino-acid analysis and amino-acid sequence analysis. we could identify all the peptides which were expected from the pro-major basic protein cdna sequence. sequence analysis and deglycosylati ...	1993	8507662
phosphatidylcholine-specific phospholipase c from achromobacter xylosoxidans.	a new phosphatidylcholine-specific phospholipase c (ec 3.1.4.3.) has been isolated from the culture broth of achromobacter xylosoxidans. growth conditions were optimized for maximum production of the enzyme. a temperature-sensitive synthesis was established. chromatography on cm sephadex and polyacrylamide gel electrophoresis of the native enzyme revealed a number of isozymes. the water soluble substrate for phospholipase p-nitrophenylphosphorylcholine was not hydrolysed by the enzyme.	1993	8511996
purification and characterization of clostridium perfringens 120-kilodalton collagenase and nucleotide sequence of the corresponding gene.	clostridium perfringens type c ncib 10662 produced various gelatinolytic enzymes with molecular masses ranging from approximately 120 to approximately 80 kda. a 120-kda gelatinolytic enzyme was present in the largest quantity in the culture supernatant, and this enzyme was purified to homogeneity on the basis of sodium dodecyl sulfate-polyacrylamide gel electrophoresis. the purified enzyme was identified as the major collagenase of the organism, and it cleaved typical collagenase substrates such ...	1994	8282691
x-ray structure and site-directed mutagenesis of a nitrite reductase from alcaligenes faecalis s-6: roles of two copper atoms in nitrite reduction.	nitrite reductase (nir) from the denitrifying bacterium alcaligenes faecalis s-6 is a copper-containing enzyme which requires pseudoazurin, a low molecular weight protein containing a single type i copper atom, as a direct electron donor in vivo. crystallographic analysis shows that nir is a trimer composed of three identical subunits, each of which contains one atom of type i copper and one atom of type ii copper, and that the ligands to the type i and type ii copper atoms are the same as those ...	1994	8172899
purification and characterization of bothrombin, a fibrinogen-clotting serine protease from the venom of bothrops jararaca.	a fibrinogen-clotting enzyme (bothrombin) was purified from the venom of bothrops jararaca. bothrombin showed m(r) values of 33,000 under nonreducing and 35,000 under reducing conditions on sds polyacrylamide gel electrophoresis and specific fibrinogen-clotting activity equivalent to 814-904 nih alpha-thrombin units/mg. diisopropyl fluorophosphate totally abolished its activity, but hirudin, a specific alpha-thrombin inhibitor, had negligible effect on bothrombin activity. unlike alpha-thrombin, ...	1994	8110787
crucial role of intralobe peptide-peptide interactions in the uptake and release of iron by ovotransferrin.	the mechanism for reversible iron binding in the n-terminal lobe of ovotransferrin was investigated by a protein fragmentation approach. the iron-saturated n-terminal half-molecule of ovotransferrin was proteolyzed into large 30-kda (1-279) and small 6-kda (280-332) fragments by a single cleavage with achromobacter protease i, producing a stable nicked form. for the separation of the two fragments, denaturing conditions were required. the isolated large fragment contained all four iron-coordinat ...	1994	8120023
delta 3, delta 2-enoyl-coa isomerase is the protein that copurifies with human glutathione s-transferases from s-hexylglutathione affinity matrices.	an unidentified 30 kda protein frequently copurifies with human glutathione s-transferases from s-hexyl-glutathione affinity matrices. application of two-step sequential affinity chromatographic methods yielded a homogeneous preparation of that protein from human liver specimens. the protein was digested with achromobacter protease i, and sequences of peptides resolved by h.p.l.c. showed a high degree of identity with those of rat mitochondrial delta 3, delta 2-enoyl-coa isomerase. the human pro ...	1994	7818490
lysyl endopeptidase of achromobacter lyticus.		1994	7845202
metabolic and structural changes in pseudomonas aeruginosa, achromobacter cdc and agrobacterium radiobacter cells injured in parenteral fluids.	the long term metabolic changes of three oxidase positive microorganisms (pseudomonas aeruginosa, agrobacterium radiobacter and achromobacter cdc) all isolated from aquatic environment, were defined after they were inoculated in three parenteral fluids: lactated ringer's solution, sodium chloride 0.9% and dextrose 5%. the number of microorganisms introduced into the parenteral products was adjusted to 10(5) bacteria/ml and left at room temperature (20-22 degrees c) for 30 days. their enzymatic a ...	1994	7850451
evidence for conformational change of fatty acid-binding protein accompanying binding of hydrophobic ligands.	conformational change of rat liver fatty acid-binding protein was investigated by making use of change of protease-susceptibility in the presence or absence of bound oleic acid and clofibrate. delipidated fatty acid-binding protein was rapidly digested by achromobacter lysyl endopeptidase, bovine alpha-chymotrypsin, and staphylococcal v8 protease, while protein recombined with oleic acid was strongly refractory to proteolysis. this observation indicates that a striking change in the conformation ...	1994	7896729
replacement of methionine as the axial ligand of achromobacter cycloclastes cytochrome c554 at high ph values revealed by absorption, epr and mcd spectroscopy.	cytochrome c554 from the denitrifying bacterium achromobacter cycloclastes is a monoheme class ii c-type cytochrome with a his-met axial coordination at neutral ph. the amino acid composition and the n-terminal sequence of the cytochrome have been determined. subsequent determination of the ph-dependence of the redox potential and examination of the epr and mcd spectra of ferricytochrome c554 revealed a new form at high ph values made apparent with both spectroscopies. these observations are con ...	1994	7945350
cloning and nucleotide sequence analysis of the colh gene from clostridium histolyticum encoding a collagenase and a gelatinase.	the colh gene encoding a collagenase was cloned from clostridium histolyticum jcm 1403. nucleotide sequencing showed a major open reading frame encoding a 116-kda protein of 1,021 amino acid residues. the deduced amino acid sequence contains a putative signal sequence and a zinc metalloprotease consensus sequence, hexxh. a 116-kda collagenase and a 98-kda gelatinase were copurified from culture supernatants of c. histolyticum. while the former degraded both native and denatured collagen, the lat ...	1994	7961400
handwashing machines, handwashing compliance, and potential for cross-contamination.	although handwashing is considered an important factor in the prevention of nosocomial infections, the optimal technique has not been determined and compliance is often difficult to obtain. handwashing compliance is particularly important in intensive care areas of the hospital. in an effort to improve hw compliance, the surgical intensive care unit in our hospital purchased three handwashing machines. four months after installation of the handwashing machines, an outbreak of methicillin-resista ...	1994	7985823
enzymatic regioselective acylation of 3-o-beta-d-galactopyranosyl-sn-glycerol by achromobacter sp. lipase.		1994	8004727
identification of three catalytic triad constituents and asp-225 essential for function of lysine-specific serine protease, achromobacter protease i.	achromobacter protease i is a lysine-specific serine protease that achromobacter lyticus m497-1 extracellularly secretes. the structural aspects necessary for the protease to function were investigated by means of site-directed mutagenesis to identify the constituents of the catalytic triad and the amino acid residue responsible for lysine specificity. the precursor molecules, which were produced by substitution of his-57, asp-113, or ser-194 for alanine, could not be converted to the mature for ...	1994	8006007
cloning and expression of the gene for hydroxypyruvate reductase (d-glycerate dehydrogenase from an obligate methylotroph hyphomicrobium methylovorum gm2.	the gene encoding hydroxypyruvate reductase, catalyzing the asymmetric reduction of hydroxypyruvate to d-glycerate, and its flanking regions were isolated from a methylotrophic bacterium, hyphomicrobium methylovorum gm2. nucleotide sequencing of the recombinant plasmids revealed that the hydroxypyruvate-reductase gene codes for the 322-amino-acid protein with calculated molecular mass 35,726 da. the sequence was confirmed by sequencing the intact enzyme and peptides obtained by digestion of the ...	1994	8055948
survey on antibiotic resistance in gram-negative non-fermentative bacteria other than pseudomonas in italy.	a survey was conducted to investigate the incidence and antibiotic resistance of clinical isolates of gram-negative non-fermentative bacteria, isolated during a one-year period in italy. overall, these microorganisms account respectively for 11.47% and 20.5% of all gram-negative bacteria. the most representative species isolated during the study were: acinetobacter, flavobacterium, alcaligenes, achromobacter and xanthomonas species. as regards their antimicrobial pattern of resistance, this surv ...	1994	8071672
alternatively spliced isoform of p-selectin is present in vivo as a soluble molecule.	to demonstrate the presence of a soluble isoform of p-selectin predicted from cdna sequencing (johnston, g.i., bliss, g.a., newman, p.j., and mcever, r.p. (1990) j. biol. chem. 265, 21381-21385), we immunoisolated and compared structurally p-selectin from fresh frozen human plasma with that from washed intact platelets. plasma p-selectin was reactive with rabbit antiserum to a synthesized peptide (residues 762-774 of mature p-selectin) but was significantly less reactive with antibody to a pepti ...	1994	7522232
hydrolysis of s-2-aminoethylcysteinyl peptide bond by achromobacter protease i.	the substrate specificity of achromobacter protease i (api) was examined for s-2-aminoethyl(ae)cysteinyl bonds in bz-aec-ome/oet, bz-aec-nh2, and ae-insulin b chain. the protease hydrolyzed all of the tested ae-cysteinyl bonds at the same rate as that of lysyl bonds. kinetic parameters were estimated for this hydrolysis reaction.	1994	7764517
amino acid sequences of double-headed proteinase inhibitors from the seeds of canavalia lineata.	the amino acids of two bowman-birk type proteinase inhibitors (clti-i and -ii) from the seeds of canavalia lineata were sequenced by a manual edman degradation using the dabitc/pitc double coupling method after enzymatic digestions with achromobacter lyticus lysyl endopeptidase, staphylococcus aureus v8 protease, and chymotrypsin. clti-i contains 75 amino acid residues. clti-ii has an identical sequence to clti-i except an extra asp residue attached at the c-terminus. the inhibitors showed a hom ...	1994	7764547
property and amino acid sequence of a subtilisin inhibitor from seeds of beach canavalia (canavalia lineata).	a subtilisin inhibitor was purified from the seeds of canavalia lineata by ammonium sulfate precipitation, ultrafiltration on a ym-30 membrane, column chromatography on deae-toyopearl and sp-toyopearl, followed by reverse-phase hplc. the inhibitor (clsi-i) is a low molecular weight protein (m(r) about 6500) containing no half-cystine residue, and quite stable as to extreme heat and ph treatment. clsi-i inhibited subtilisin-type serine proteases including s. griseus alkaline protease. the amino a ...	1994	7765594
transfer and expression of pcb-degradative genes into heavy metal resistant alcaligenes eutrophus strains.	sites polluted with organic compounds frequently contain inorganic pollutants such as heavy metals. the latter might inhibit the biodegradation of the organics and impair bioremediation. chromosomally located polychlorinated biphenyl (pcb) catabolic genes of alcaligenes eutrophus a5, achromobacter sp. lbs1c1 and alcaligenes denitrificans jb1 were introduced into the heavy metal resistant alcaligenes eutrophus strain ch34 and related strains by means of natural conjugation. mobile elements contai ...	1994	7765842
structure of azurin from achromobacter xylosoxidans ncib11015 at 2.5 a resolution.	the crystal structure of azurin from a denitrifying bacterium, achromobacter xylosoxidans ncib11015, has been refined at 2.5 a resolution using diffraction data obtained by means of synchrotron radiation at kek. crystals suitable for x-ray experiment were obtained by the macro-seeding method and an intensity data were obtained on imaging plates mounted on a weissenberg camera (rmerge = 0.09). the initial model was obtained by the molecular replacement method using the structure of azurin from al ...	1994	7706206
site-specific glycosylation of bovine butyrophilin.	our previous studies showed that the n-linked sugar chains of most bovine glycoproteins from milk fat globule membranes (mfgm) contain the galnac beta 1-->4glcnac group [sato et al. (1993) j. biochem. 114, 890-900]. since expression of the disaccharide structure is influenced by peptide sequences near the glycosylation sites [smith and baenziger (1992) proc. natl. acad. sci. usa 89, 329-333], the site-specificity of the n-acetylgalactosaminylated sugar chains was investigated using bovine butyro ...	1995	7775382
structure of alcaligenes faecalis nitrite reductase and a copper site mutant, m150e, that contains zinc.	the structures at 2.0 and 2.25 a resolution of native and recombinant nitrite reductase from alcaligenes faecalis show that they are identical to each other and very similar to nitrite reductase from achromobacter cycloclastes. the crystallographic structure of a mutant, m150e, which unlike the wild-type protein cannot be reduced by pseudoazurin, shows that the glutamate replacement for methionine binds to a metal at the type i cu site via only one oxygen. anomalous scattering data collected at ...	1995	7547950
identification of the electrophilic substrate-binding site of glutathione s-transferase p by photoaffinity labeling.	we determined the electrophilic substrate-binding site of rat glutathione s-transferase p (gst-p) by photoaffinity labeling using the photosensitive compound s-[2-(2-fluoro-4-nitrophenoxy)ethyl]glutathione. this photosensitive glutathione analogue inhibited the catalytic activity in a competitive manner against both glutathione and 1-chloro-2,4-dinitrobenzene, a putative electrophilic substrate. the enzyme kinetics indicated that the photoactivatable glutathione analogue was specifically bound a ...	1995	7556138
[comparative susceptibility of ochrobactrum anthropi, agrobacterium tumefaciens, alcaligenes faecalis, alcaligenes denitrificans subsp. denitrificans, alcaligenes denitrificans subsp. xylosidans and bordetella bronchiseptica against 35 antibiotics including 17 beta-lactams].	ochrobactrum anthropi, formerly known as "achromobacter sp." or cdc group vd has been isolated from water, hospital environment (antiseptic solutions, dialysis fluids ... ). o. anthropi is a gram negative, motile, strictly aerobic, oxydase positive and non-fermentative bacteria with a strong urease activity. the susceptibility of 13 strains of o. anthropi was determined by agar diffusion method and compared to those of type strains of agrobacterium tumefaciens, alcaligenes faecalis, alcaligenes ...	1995	7567111
spectroscopic and electrochemical studies on active-site transitions of the type 1 copper protein pseudoazurin from achromobacter cycloclastes.	the single type 1 copper protein pseudoazurin from achromobacter cycloclastes gives reversible electrochemical behavior at a (4-pyridyl)disulfide-modified gold electrode. measurements carried out at 25.0 degrees c indicate a midpoint reduction potential of e 1/2 = 260 mv versus normal hydrogen electrode at ph 7.0 and a peak-to-peak separation of delta ep = 59 mv. the diffusion coefficient and heterogeneous electron transfer rate constant are estimated to be 2.23 x 10(-6) cm2 s-1 and 3.7 x 10(-2) ...	1995	7592754
screening and characterization of microorganisms with glutaryl-7adca acylase activity.	a screening of microorganisms producing glutaryl-7adca acylase, an enzyme able to hydrolyse glutaric acid selectively from glutaryl-3-deacetoxy-7-aminocephalosporanic acid (glutaryl-7adca), has been carried out in soil samples. five microorganisms expressing acylase activity were isolated and classified as bacillus cereus, achromobacter xylosooxidans, bacillus sp., pseudomonas sp. and pseudomonas paucimobilis. the screening was carried out by preparing enrichment cultures containing glutaryl-7-a ...	1995	7632401
the complete amino acid sequence of a mannose-binding lectin from "kidachi aloe" (aloe arborescens miller var. natalensis berger).	the complete amino acid sequence of a mannose-binding lectin purified from the leaf skin of "kidachi aloe" (aloe arborescens miller var. natalensis berger) is presented. the 109-residue sequence of the subunit was determined by analysis of peptides of the intact or s-pyridylethylated protein generated by digestion with cyanogen bromide, bnps-skatole, achromobacter protease i, or trypsin. the subunit contains an intrachain disulfide bridge. the sequence is highly homologous to that of a mannose-b ...	1995	7669035
[infection in the dialysis unit as a contributing factor to the problem of hospital acquired infections].	hospital acquired infections are especially dangerous for patients with chronic renal insufficiency. an epidemic of hospital acquired infection of dramatic course in described in 13 patients (8 being on haemodialysis (hd) and 5 treated conservatively), with 15 episodes of septic syndrome (12 cases) or septic shock (3 cases). initial cultures of blood, urine and pharyngeal swabs were positive only in 5 patients treated conservatively. initial cultures of dialysing fluid from all 12 monitors of hd ...	1995	7479256
amino acid sequencing, molecular cloning and modelling of the chick liver class-theta glutathione s-transferase cl1.	glutathione s-transferase cl1-2 heterodimers purified from 1-day-old chick livers were digested with achromobacter proteinase i. the resulting fragments were separated for amino acid sequence analysis. oligonucleotide probes were constructed based on sequence similarity to class-theta glutathione s-transferases for pcr using a chicken liver cdna library as template. a full-length clone (1725 bp) encoding a polypeptide comprising 261 amino acids was isolated. including conservative substitutions, ...	1995	7492340
the structure of copper-nitrite reductase from achromobacter cycloclastes at five ph values, with no2- bound and with type ii copper depleted.	high resolution x-ray crystallographic structures of nitrite reductase from achromobacter cycloclastes, undertaken in order to understand the ph optimum of the reaction with nitrite, show that at ph 5.0, 5.4, 6.0, 6.2, and 6.8, no significant changes occur, other than in the occupancy of the type ii copper at the active site. an extensive network of hydrogen bonds, both within and between subunits of the trimer, maintains the rigidity of the protein structure. a water occupies a site approximate ...	1995	7499203
dephosphorylation of fetal-tau and paired helical filaments-tau by protein phosphatases 1 and 2a and calcineurin.	we have reported that many sites of tau in fetal brain (fetal-tau) as well as in paired helical filaments (phf-tau) are phosphorylated. in the present study, we used site-specific antibodies and peptide mapping to examine protein phosphatases involved in dephosphorylation of fetal-tau and phf-tau. immunoblot analysis and electrophoretic mobility showed that protein phosphatases 1 and 2a and calcineurin could dephosphorylate fetal-tau and phf-tau. phosphoserines 199, 202, 396, and 413 and phospho ...	1995	8720139
purification and primary structure of a myotoxic lysine-49 phospholipase a2 with low lipolytic activity from trimeresurus gramineus venom.	four acidic phospholipase a2 (pla2) isozymes named pla2-i, ii, iii and iv have previously been isolated from trimeresurus gramineus (green habu snake) venom and sequenced [oda et al. (1991) toxicon 29, 157; fukagawa et al. (1992) toxicon 30, 1131; fukagawa et al. (1993) toxicon 31, 957]. they contain aspartate-49 which is known to bind ca2+, essential for catalysis. in the present study, a basic pla2 named pla2-v containing lysine-49 was newly isolated from the same snake venom. its isoelectric ...	1995	8744986
meningitis caused by alcaligenes (achromobacter) xylosoxidans associated with epidural catheter.		1995	8655217
human colorectal carcinomas specifically accumulate mr 42,000 ubiquitin-conjugated cytokeratin 8 fragments.	recent studies have shown that various tumor cells accumulate ubiquitin (ub)-conjugated proteins, the profiles of which differ from those of normal cells. to identify the ub-conjugated proteins accumulated specifically by human carcinoma cells, a two-dimensional immunoblot analysis of 31 surgically resected human primary colorectal carcinoma tissues was performed using an anti-ub monoclonal antibody, km691. two distinct mr 42,000 and 45,000 proteins in the triton x-insoluble fractions of carcino ...	1996	8665509
high-resolution crystal structures of two polymorphs of cytochrome c' from the purple phototrophic bacterium rhodobacter capsulatus.	the structures of two polymorphs of cytochrome c' from rhodobacter capsulatus (rccp) strain m110 have been determined by the molecular replacement method. iron anomalous scattering data were used to confirm the molecular replacement solution. the structures were refined at 1.72 angstrom and 2.0 angstrom resolution to r-values of 15.0% and 16.3%, respectively. the rccp molecule is a dimer and each of the identical 129 residue subunits folds as a four-helical bundle with a covalently bound heme gr ...	1996	8676382
crystallization and preliminary x-ray diffraction analysis of two lysinal derivatives of achromobacter protease i.	two crystal forms of lysinal derivatives of achromobacter protease i have been obtained. the first, modified by benzyloxycarbonyl-val-lysinal crystallizes in the monoclinic space group p2(1) with unit-cell dimensions of a = 39.6, b = 71.2, c = 45.6 a and beta = 98.4 degrees. the second, modified by benzyloxycarbonyl-leu-leu-lysinal crystallizes in the orthorhombic space group i222 (or i2(1)2(1)2(1)) with unit-cell dimensions of a = 98.7, b = 102.2 and c = 55.8 a. the space groups and the unit-ce ...	1996	15299616
impact of a genetically engineered bacterium with enhanced alkaline phosphatase activity on marine phytoplankton communities.	an indigenous marine achromobacter sp. was isolated from coastal georgia seawater and modified in the laboratory by introduction of a plasmid with a phoa hybrid gene that directed constitutive overproduction of alkaline phosphatase. the effects of this "indigenous" genetically engineered microorganism (gem) on phosphorus cycling were determined in seawater microcosms following the addition of a model dissolved organic phosphorus compound, glycerol 3-phosphate, at a concentration of 1 or 10 (mu)m ...	1996	16535222
[bacteremia caused by achromobacter xylosidans].		1996	8750544
s-pyridylethylation of intact polyacrylamide gels and in situ digestion of electrophoretically separated proteins: a rapid mass spectrometric method for identifying cysteine-containing peptides.	in-gel proteolytic digestion of acrylamide-gel separated proteins is a method widely used for generating peptide fragments for the purpose of identifying proteins by edman degratation, tandem mass spectrometry, and peptide-mass fingerprinting. however, it is well recognised for disulfide-bonded proteins electrophoresed under reducing conditions that if no precautions are taken to minimise disulfide bond formation during protein digestion or peptide isolation, complex peptide maps can result. her ...	1996	8783016
incidence of pyrexia in patients undergoing haemodialysis.	four patients on maintenance haemodialysis at the lagos university teaching developed pyrogenic reactions during treatment. blood cultures were negative but the haemodialysates were grossly contaminated, mostly with gram-negative bacilli, implicating their endotoxin as the cause of the observed pyrogenicity. the water reservoirs supplying the dialysis centre were grossly contaminated (counts 2.0 x 10(2) cfuml-1) with gram-negative bacteria such as pseudomonas aeruginosa, ps. fluorescens, alcalig ...	1996	8855673
bordetella trematum sp. nov., isolated from wounds and ear infections in humans, and reassessment of alcaligenes denitrificans rüger and tan 1983.	ten strains recognized on the basis of a computer-assisted numerical comparison of whole-cell protein patterns as members of a novel species belonging to the family alcaligenaceae were examined by using an integrated phenotypic and genotypic approach. this species, for which we propose the name bordetella trematum sp. nov., was more closely related to the type species of the genus bordetella (bordetella pertussis) than to the type species of the genus alcaligenes (alcaligenes faecalis) and had t ...	1996	8863408
systematic peptide fragmentation of polyvinylidene difluoride(pvdf)-immobilized proteins prior to microsequencing.	the sequential in situ digestion of proteins immobilized on polyvinylidene difluoride (pvdf) has been systematically designed and optimized. the method consists of immobilization of the proteins on pvdf, s-carboxymethylation, and then successive in situ digestions using specific proteases. in order to obtain high yields of the peptide fragments, from which specific amino acid residues connected to the n- or c-terminal of the resulting digestion fragments can be deduced, the cleavages are perform ...	1996	8864840
achromobacter xylosoxidans bacteremia: report of four cases and review of the literature.	seventy-seven cases of bacteremia due to achromobacter xylosoxidans were reviewed, and susceptibility studies were performed on 11 clinical isolates of a. xylosoxidans. nosocomial bacteremia was noted in 54 of 77 patients (70%), and 28 (36%) had infection associated with an outbreak or acquired from a discrete point source. the most common underlying illnesses were malignancies (30%) and cardiac disease (21%); immunosuppression affected 27%. the most common clinical syndromes were primary and ca ...	1996	8879782
identification and partial amino acid sequences of seven s-rnases associated with self-incompatibility of japanese pear, pyrus pyrifolia nakai.	s-allele-specific proteins (s-proteins) were separated and identified by two-dimensional (2d) gel electrophoresis from the style extract of 14 cultivars of japanese pear, pyrus pyrifolia nakai, which exhibits gametophytic self-incompatibility. these s-proteins were 30-32 kda basic proteins with putative pis of 9.6-10.1 and were distinct from the other proteins, which were common for all cultivars examined. each s-protein was assigned to a given s-genotype based on electrophoretic mobility and th ...	1996	8889818
a substitution at his-120 in the lasa protease of pseudomonas aeruginosa blocks enzymatic activity without affecting propeptide processing or extracellular secretion.	the lasa protease of pseudomonas aeruginosa can degrade elastin and is an important contributor to the pathogenesis of this organism. lasa (20 kda) is a member of the beta-lytic endopeptidase family of extracellular bacterial proteases, and it shows high-level staphylolytic activity. we sequenced the lasa gene from strain frd1 and overexpressed it in escherichia coli. the lasa gene encodes a precursor, known as pre-prolasa, of 45,582 da. amino-terminal sequence analysis allowed the identificatio ...	1996	8932318
[bacteremia caused by alcaligenes (achromobacter) xylosoxidans. description of 3 cases and review of the literature].	alcaligenes (achromobacter) xylosoxidans occasionally cause infections, mainly in immunocompromised hosts.	1996	8991439
[collagenolytic enzymes synthesized by microorganisms].	the review uses data obtained by the authors and available in the literature to discuss microbial collagenolytic enzymes, widely employed in scientific research, biotechnology, and medicine. collagenases differing in their structure and the specificity of their action on collagen fibrils were isolated from bacteria (including marine isolates), actinomycetes, and fungi. collagenases produced by clostridium histolyticum and achromobacter iophagus are the best studied enzymes; both are metalloenzym ...	1996	8992239
location of cysteine and cystine residues in s-ribonucleases associated with gametophytic self-incompatibility.	s-ribonucleases (s-rnases) that cosegregate with s-alleles in the styles of solanaceous and rosaceous plants are associated with gametophytic self-incompatibility (gsi). the amino acid sequences of many s-rnases have been derived from cdna sequences, but the state of half-cystines has not been clarified. we report the locations of the two free cysteine residues and four disulfide bridges of tobacco s6-rnase and of the four disulfide bridge of japanese pear s4-rnase. the protein was first s-pyrid ...	1996	9022690
the 30 kda protein co-purified with chick liver glutathione s-transferases is a carbonyl reductase.	an unidentified 30 kda protein was co-purified with chick liver glutathione s-transferases from s-hexylglutathione affinity column. the protein was isolated to apparent homogeneity with chromatofocusing. the molecular mass of the protein was determined to be 30 277 +/- 3 dalton by mass spectrometry. the protein was digested with achromobacter proteinase i. amino-acid sequence analyses of the resulting peptides show a high degree of identity with those of human carbonyl reductase. the protein is ...	1996	8597575
cloning, characterization, and expression of the nitric oxide-generating nitrite reductase and of the blue copper protein genes of achromobacter cycloclastes.	the nitrite reductase (nir) and blue copper protein (bcp) genes have been cloned from achromobacter cycloclastes and characterized. nir gene encodes a protein of 378 amino acid residues including a putative signal peptide of 37 residues. bcp gene encodes a protein of 148 residues with a 24-residue signal peptide. the dna-derived amino acid sequence of nir is in complete agreement with that from edman degradation and the dna coding sequence of bcp is also consistent with its partial n-terminal am ...	1996	8605003
a removable spacer peptide in an alpha-factor-leader/insulin precursor fusion protein improves processing and concomitant yield of the insulin precursor in saccharomyces cerevisiae.	an alpha-factor leader/insulin precursor fusion protein was produced in saccharomyces cerevisiae and metabolically labeled in order to analyse the efficiency of maturation and secretion. a substantial fraction of the secreted material was found in a hyperglycosylated unprocessed form, indicating incomplete kex2p endopeptidase maturation. introduction of a spacer peptide (eaeaeak) after the dibasic kex2p site, creating a n-terminal extension of the insulin precursor, greatly increased the kex2p c ...	1996	8621069
complete amino acid sequence and identification of the platelet glycoprotein ib-binding site of jararaca gpib-bp, a snake venom protein isolated from bothrops jararaca.	jararaca gpib-bp, a snake venom protein composed of alpha and beta subunits purified from bothrops jararaca, binds to platelet glycoprotein (gp)ib and functions as a receptor blocker for von willebrand factor binding to gpib (fujimura, y., ikeda, y., miura, s., yoshida, e., shima, h., nishida, s., suzuki, m., titani, k., taniuchi, y., and kawasaki, t. (1995) thromb. haemostasis 74, 743-750). we present here the entire 142- and 123-residue amino acid sequence of the respective alpha and beta subu ...	1996	8631868
characterization and distribution of o-glycosylated carbohydrates in the cell adhesion molecule, contact site a, from dictyostelium discoideum.	this paper presents further investigation of the properties of carbohydrate ii in the cell adhesion molecule, contact site a, from dictyostelium discoideum. a purified contact site a was digested with achromobacter protease i to produce a 31-kda fragment to which carbohydrate ii was mainly bound and a 21-kda fragment containing the nh2 terminus of contact site a, which was identified as ala-pro-thr-ile-thr-ala. the nh2 terminus of the 31-kda fragment was thr-glu-ala-thr-thr-ser. it was estimated ...	1997	9024799
nitrite reductase from achromobacter xylosoxidans forms stable trimers in dilute solution.		1997	9056938
recurrent septicaemia due to "achromobacter group b".		1997	9138138
d-lysine production from l-lysine by successive chemical racemization and microbial asymmetric degradation.	in order to develop a practical process for d-lysine production from l-lysine, successive chemical racemization and microbial asymmetric degradation were investigated. the racemization of l-lysine proceeded quantitatively at elevated temperatures. a sample of 1000 strains of bacteria, fungi, yeast and actinomyces were screened for the ability to degrade l-lysine asymmetrically. microorganisms belonging to the achromobacter, agrobacterium, candida, comamonas, flavobacterium, proteus, providencia, ...	1997	9163947
evidence for increased hydroxylation of pyrrolidone amino acid residues in the cuticle of mature onchocerca volvulus.	to determine whether morphologic changes are accompanied by variations in the biochemical and antigenic properties of the cuticle of onchocerca volvulus during development, we isolated and compared the 2-mercaptoethanol soluble cuticular proteins and the insoluble cuticlin from the predominant life-cycle stages occurring in man. sds-page analysis, before and after digestion with collagenase from achromobacter iophagus, revealed that the polypeptide composition of the 2-mercaptoethanol-solubilise ...	1997	9197461
intravenous line infection due to ochrobactrum anthropi (cdc group vd) in a normal host.	ochrobactrum anthropi, formerly known as achromobacter species (cdc group vd), is an aerobic, gram-negative bacillus widely distributed in aquatic environments. most important, it has been implicated as a cause of intravenous line infection in immunocompromised hosts with solid tumors or hematologic malignancies. trimethoprim-sulfamethoxazole and aminoglycosides are usually active against o. anthropi, but this organism is usually resistant to beta-lactam antibiotics. because o. anthropi is a low ...	1997	9257145
sulfated lipids as inhibitors of pancreatic trypsin and chymotrypsin in epithelium of the mammalian digestive tract.	cholesterol sulfate and i3so3-galcer, which were commonly contained in the epithelia of mammalian digestive tracts, were found to inhibit the activities of typical digestive enzymes, pancreatic trypsin and chymotrypsin, but steroid sulfates, gangliosides and the other membrane constituents did not show any inhibitory activity. the preincubation of trypsin with i3so3-galcer at 37 degrees c for 10min was necessary to inhibit the activity of trypsin, but cholesterol sulfate showed its inhibitory ac ...	1997	9268697
domon l, an immunopotentiating agent, acts as an nf-kappab activator.	domon l, a heat-treated component of gram-negative bacterium achromobacter stenohalis, is used for the treatment of infectious diseases of animals. here, we investigated the immunopotentiating potential of domon l. in vitro studies showed that domon l enhanced nitric oxide (no) formation from murine macrophage raw264.7 cells in concert with interferon (ifn)-gamma. the effect of domon l on nf-kappab activation was investigated, in order to understand the molecular mechanisms of enhanced no format ...	1997	9271453
characterization of the gene encoding catechol 2,3-dioxygenase from achromobacter xylosoxidans kf701.	catechol 2,3-dioxygenase (c23o) catalyzes a meta cleavage of the aromatic ring in catechol to form 2-hydroxymuconic semialdehyde. a c23o gene was cloned from chromosomal dna of a. xylosoxidans kf701, a soil bacterium degrading biphenyl, and expressed in e. coli hb101. in substrate specificity to catechol and its analogs, the c23o exhibited the highest aromatic ring-fission activity to catechol, and its relative activity to other dihydroxylated aromatics was 4-chlorocatechol > 4-methylcatechol > ...	1997	9299526
structure of a covalently cross-linked form of core histones present in the starfish sperm.	the post-translational modification of core histones plays an essential role in chromatin remodeling processes. we recently reported the occurrence of a novel histone modification, involving a epsilon-(gamma-glutamyl)lysine cross-link between a glutamine residue of histone h2b and a lysine residue of histone h4 in the testis of the starfish, asterina pectinifera[shimizu, t., hozumi, k., horiike, s., nunomura, k., ikegami, s., takao, t., and shimonishi, y. (1996) nature 380, 32]. in order to dete ...	1997	9315845
purification, characterization, and amino acid sequencing of dnase gamma from rat spleen.	an endonuclease named dnase gamma was purified to apparent homogeneity from rat splenocyte nuclei and its properties were characterized. we also determined the nh2-terminal and partial amino acid sequences of the proteolytic internal peptides. the molecular mass of gamma dnase was 33,000 daltons as determined by sds-polyacrylamide gel electrophoresis. a native molecular mass of 30,000 was estimated by gel filtration. purified dnase gamma is active in the presence of both ca2+ and mg2+ or mn2+ al ...	1997	9328279
using monoclonal antibodies to characterize a sequential epitope on the group i allergen of bermuda grass pollen.	cyn d 1, the group i allergen of bermuda grass pollen, had been purified and characterized.	1997	9363907
purification, staphylolytic activity, and cleavage sites of alpha-lytic protease from achromobacter lyticus.	alpha-lytic protease (alp) was purified from a bacteriolytic agent, achromopeptidase from achromobacter lyticus m497-1, and has been shown to possess staphylolytic activity. cleavage sites of this enzyme on the peptidoglycan of staphylococcus aureus were determined by n-terminal amino acid sequence and amino acid composition analyses. alp cleaved the n-acetylmuramoyl-l-alanine amide bond, the junction between the polysaccharide and peptide moieties, in addition to the d-ala-gly and gly-gly pepti ...	1997	9399581
bombyx mori nucleopolyhedrovirus encodes a dna-binding protein capable of destabilizing duplex dna.	a dna-binding protein (designated dbp) with an apparent molecular mass of 38 kda was purified to homogeneity from bmn cells (derived from bombyx mori) infected with the b. mori nucleopolyhedrovirus (bmnpv). six peptides obtained after digestion of the isolated protein with achromobacter protease i were partially or completely sequenced. the determined amino acid sequences indicated that dbp was encoded by an open reading frame (orf16) located at nucleotides (nt) 16189 to 17139 in the bmnpv genom ...	1998	9525636
achromobacter xylosoxidans bacteremia in patients with hematologic malignancies.	we report nine cases of achromobacter xylosoxidans bacteremia diagnosed in patients with hematologic malignancies. there was not an obvious epidemiologic link between cases and the organism was not isolated from any source. outcome was cure in all nine cases. in our experience, catheter removal is generally required for eradication of a. xylosoxidans.	1998	9573685