Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| enrichment medium for isolation of campylobacter jejuni-campylobacter coli. | a broth enrichment medium for the improvement of isolation of campylobacter jejuni-campylobacter coli from stool samples and other specimens is presented. of 1,228 samples examined in parallel, positive results were obtained from 81 by direct inoculation of selective media and from 112 after enrichment. thus, an increase of 27.7% in the isolation rate was obtained by using the enrichment medium. the same medium without antibiotics allows the preservation of isolates of c. jejuni-c. coli for at l ... | 1985 | 4026287 |
| transmission of campylobacter jejuni by the housefly (musca domestica). | houseflies (musca domestica) were infected with campylobacter jejuni after being confined for 5 days in a horsfall isolator containing 25-day-old chickens known to be fecal excretors of the organism. contaminated flies, when subsequently transferred to a second unit, transmitted c. jejuni to specific-pathogen-free chickens. allowing a sample of 32 houseflies to ingest c. jejuni in a liquid suspension resulted in recovery rates of 20% from the feet and ventral surface of the body and 70% from the ... | 1985 | 4026732 |
| role of litter in the transmission of campylobacter jejuni. | autoclaved or non-autoclaved used broiler litter that was experimentally contaminated with campylobacter jejuni was capable of infecting specific-pathogen-free chicks maintained in modified horsfall isolators. artificially infected chicks became fecal shedders of c. jejuni, resulting in contamination of both autoclaved and non-autoclaved used broiler litter. fecal shedding of c. jejuni by litter-reared, artificially infected chicks persisted for at least 63 days after chicks were transferred to ... | 1985 | 4026733 |
| community-wide surveillance of campylobacter jejuni infection. evaluation of a laboratory-based method. | surveillance of infections due to campylobacter jejuni is needed to further define the epidemiology of this disease in the u.s. we assessed one potential method of community-wide surveillance, a laboratory-based reporting network. six microbiology laboratories that routinely culture fecal specimens for c. jejuni reported all isolates to seattle-king county health department for an 18-month period. further investigations on 476 cases reported from this broadly based network revealed that c. jejun ... | 1985 | 4028667 |
| campylobacter colitis: histological immunohistochemical and ultrastructural findings. | the colonic biopsy specimens of 22 patients with colitis and positive stool cultures for campylobacter jejuni were studied in order to obtain histological and immunohistochemical criteria to differentiate campylobacter colitis from chronic inflammatory bowel disease. in addition we tried to identify campylobacter inclusions by means of immunohistochemistry and electron microscopy as evidence for invasion of the colonic mucosa. the results show that the majority of patients with campylobacter col ... | 1985 | 4029720 |
| biotyping schemes for campylobacter jejuni. | 1985 | 4031047 | |
| biotypes and serotypes of campylobacter jejuni and campylobacter coli strains isolated from patients, pigs and chickens in the region of rotterdam. | a comparison was made of the distribution of the bio-and serotypes of campylobacter jejuni and campylobacter coli isolated from human patients (205 isolates), intestinal contents of pigs (163 isolates) and intestinal contents and livers of chickens (147 isolates). all strains were isolated in the region of rotterdam. campylobacter jejuni biotype 1 accounted for 86%, 1% and 82% of the human, pig and chicken isolates respectively; c. coli was present in 7%, 98% and 14% respectively. serotyping was ... | 1985 | 4031520 |
| serum antibodies to heat-labile enterotoxin of campylobacter jejuni. | 1985 | 4031551 | |
| a point-source outbreak of campylobacteriosis associated with consumption of raw milk. | after a one-day field trip to a minnesota farm, 22 (45%) of 49 third-grade students and three (14%) of 21 adult chaperones developed campylobacteriosis. campylobacter jejuni was isolated from specimens of 13 children and one asymptomatic adult. illness was associated with the consumption of raw milk during a picnic lunch (odds ratio = 41.0, p less than .0001) and participation in hand milking of cows (odds ratio = 37.5, less than .0001). two additional findings implicated consumption of raw milk ... | 1985 | 4031557 |
| experimental infection of hamsters with campylobacter jejuni. | campylobacter jejuni is a major cause of diarrhea and enterocolitis in humans and animals. a mammalian model has been developed for studying pathogenesis of the disease in hamsters by colonizing the ileum and cecum with c. jejuni via either oral intubation after purgation or direct surgical inoculation into the cecum. this colonization resulted in production of disease including diarrhea and intestinal lesions that resembles enterocolitis due to c. jejuni in humans and domestic animals. the muco ... | 1985 | 4038721 |
| occurrence of plasmids and antibiotic resistance among campylobacter jejuni and campylobacter coli isolated from healthy and diarrheic animals. | serologically defined strains of campylobacter jejuni and campylobacter coli from healthy and diarrheic animals were examined for the occurrence of plasmid dna in association with the antibiotic susceptibility of the bacterial host and the health status of the animal host. of all campylobacter organisms surveyed, 53% (116 of 200) contained plasmid dna. a plasmid occurrence rate of 73.8% was obtained for c. coli from healthy pigs, contrasted by lower plasmid occurrence rates for c. coli from diar ... | 1985 | 4044793 |
| hypogammaglobulinemia and recurrent campylobacter jejuni infection. | 1985 | 4045252 | |
| experimental infection of gnotobiotic mice with campylobacter jejuni: colonisation of intestine and spread to lymphoid and reticulo-endothelial organs. | axenic and monoxenic c3h mice were used to develop an animal model for enteroinvasiveness and translocation of campylobacter jejuni. after oral administration of 10(7)-10(8) viable cells of c. jejuni on day 0 (d0), bacterial colonisation was followed quantitatively during 23 days by counting free luminal bacteria and tissue-associated bacteria in the duodenum, ileum and colon. the kinetics of bacterial colonisation were the same in axenic and monoxenic mice; bacteria were more numerous in distal ... | 1985 | 4045991 |
| epidemiologic and laboratory investigation of an outbreak of campylobacter enteritis associated with raw milk. | an outbreak of gastroenteritis occurred in march-april 1981, in wichita, kansas, and involved more than 250 persons who drank raw milk from a single local dairy. campylobacter jejuni was recovered from 60 of 116 (52%) persons in households that had one or more ill family members. a cohort study of families that belonged to a food cooperative that purchased raw milk from the implicated dairy showed a significant association between illness and having drunk raw milk. thirty-nine of 55 (71%) person ... | 1985 | 4050775 |
| comparison of methods for isolating campylobacter jejuni from raw milk. | the method of doyle and roman (appl. environ. microbiol. 43:1343-1353, 1982) was compared with that of lovett et al. (appl. environ. microbiol. 46:459-462, 1983) for the ability to recover campylobacter jejuni strains inoculated into raw milk at a concentration of less than 1 cell per g. the method of lovett et al. gave significantly greater recovery proportions. | 1985 | 4051491 |
| serotyping of campylobacter jejuni from an outbreak of enteritis implicating chicken. | an outbreak of campylobacter enteritis involving 7 of 17 people over a period of 5 days followed a dinner at a restaurant. a chicken casserole dish was implicated with a food-specific attack rate of 58%. campylobacter jejuni penner serotype 18/21/29, resistant to metronidazole, was isolated from 3 of 4 symptomatic patients and from three raw fresh chicken samples closely associated with the implicated chicken. numbers of c. jejuni in the chicken ranged from 5.3 x 10(1) to 7.5 x 10(2) colony form ... | 1985 | 4056451 |
| motility as an intestinal colonization factor for campylobacter jejuni. | the colonization of the intestinal tract of suckling mice by campylobacter jejuni was examined by orally challenging the mice with a wild-type strain and several nonmotile mutant strains which were isolated after treating the wild-type strain with mutagens. the wild-type strain had colonized the lower portion of the small intestine, the caecum and the colon 2 d after inoculation. two nonmotile strains, one of which (m8) had lost all the flagellar structure including the filament, the hook and th ... | 1985 | 4056739 |
| chemically defined media for auxotyping of campylobacter jejuni. | a set of chemically defined media has been developed for the cultivation of campylobacter jejuni strains of human origin. a minimal medium, a complete medium and 5 different nutrient-deficient media (ndm1-ndm5) are described. some of the strains investigated required l-methionine(lacking in ndm1), l-cystine and l-cysteine (ndm2), k2hpo4 (ndm 3), kh2po4 (ndm4) and nad, thiamine and calcium pantothenate (ndm5). 57.7% of the strains investigated required l-methionine. the strains grew at ph 6.6-7.7 ... | 1985 | 4060921 |
| reversible expression of flagella in campylobacter jejuni. | campylobacter jejuni 81116 and a3249 undergo a bidirectional transition between flagellated (fla+) and aflagellated (fla-) phenotypes. when measured in culture medium, the fla+----fla- transition occurred at a rate of 3.1 x 10(-3) to 5.9 x 10(-3) per cell per generation, and the fla- to fla+ transition occurred at a rate of 4.0 x 10(-7) to 8.0 x 10(-7) per cell per generation. however, passage through a rabbit intestine markedly favored the fla+ phenotype. | 1985 | 4066041 |
| investigations on the role of flagella in the colonization of infant mice with campylobacter jejuni and attachment of campylobacter jejuni to human epithelial cell lines. | the biochemical and biological properties of the flagella of campylobacter jejuni have been investigated using two variants selected from a flagellate, motile clinical isolate (strain 81116): a flagellate, non-motile variant (sf-1) and an aflagellate variant (sf-2). phenotypic and biochemical analysis of the strains and amino acid analysis of the isolated flagella suggest that the variants differed from the wild-type strain only in the absence of flagella and/or motility. the aflagellate variant ... | 1985 | 4067286 |
| [seasonal distribution of campylobacter jejuni and campylobacter coli (1 september 1982--31 august 1983)]. | 1985 | 4068889 | |
| [bacteriological and serological evidence for the presence of campylobacter jejuni in poultry, animals and man (1982-1983)]. | 1985 | 4070879 | |
| comparison of gauze swabs and membrane filters for isolation of campylobacter spp. from surface water. | the epidemiology of campylobacter jejuni indicates that waterborne transmission is important; the organism has been isolated from seawater, fresh water, and estuarine sites. membrane filtration, with and without use of an enrichment broth, has been the most common method for isolating c. jejuni from water. we evaluated two methods for isolating c. jejuni from water: membrane filtration and gauze filtration. the membrane filters evaluated included 0.22- and 0.45-micron-pore millipore filters (mil ... | 1985 | 4073894 |
| inhibition of campylobacter coli and campylobacter jejuni by antibiotics used in selective growth media. | the ability of campylobacter coli and campylobacter jejuni to grow in the presence of antibiotics used in selective growth media was compared. mic data for c. coli indicated that some strains were more susceptible to the antibiotics than were the c. jejuni strains tested. a reduction of greater than 1 log cycle in the numbers of cells growing on plates containing antibiotics was considered to be a marked level of inhibition. only one of nine of the antibiotic combinations studied did not markedl ... | 1985 | 4077962 |
| [campylobacter jejuni infections: epidemiology and possibility of prevention]. | 1985 | 4079992 | |
| efficacy of media and methods for detecting and enumerating campylobacter jejuni in refrigerated chicken meat. | a study was undertaken to compare several enrichment and direct isolation media for their suitability to detect and enumerate five strains of campylobacter jejuni in refrigerated (5 degrees c) chicken meat. the influence of co2 on survival at 5 degrees c was also investigated. selective enrichment media evaluated included preston broth (pb), selective semisolid brucella medium (ssbm), campylobacter enrichment broth (ceb), vtp brucella-fbp broth (vtp), rosef and kapperud campylobacter enrichment ... | 1985 | 4083888 |
| effect of free-radical and oxygen scavengers on photochemically generated oxygen toxicity and on the aerotolerance of campylobacter jejuni. | exposure of a nutrient agar medium to the combined action of fluorescent light and air produced toxic factors in the medium which affected the growth of campylobacter jejuni. sodium dithionite (5-10 mm), a powerful reducing agent, and catalase were effective in counteracting the injurious action of light and air. among the quenchers of singlet oxygen tested, only histidine had a beneficial effect on the recovery of c. jejuni in the photo-oxidized medium, while the addition of superoxide dismutas ... | 1985 | 4086409 |
| severe campylobacter infection in children. | children suffering from campylobacter jejuni infection and who were admitted to children's hospitals in bristol and newcastle over a period of 2 years are reviewed. gastro-intestinal disease caused by c. jejuni was as common as that due to salmonella spp. three neonates suffered particularly severe infections which resembled necrotising enterocolitis. one such case is described. | 1985 | 4086864 |
| [electron microscopy study of coccoid forms of campylobacter jejuni]. | 1985 | 4089328 | |
| occurrence of campylobacter jejuni in diarrhoeic and non-diarrhoeic children in são paulo, brazil. | 1985 | 4089456 | |
| susceptibility of campylobacter jejuni and campylobacter coli to macrolides and related compounds. | the susceptibility of 105 thermophilic campylobacters from human and swine origins to eight macrolides and related compounds was tested. erythromycin, josamycin, clindamycin, and ase 136 bs (a new erythromycin derivative) were the most active against the human strains. the swine strains were highly resistant, except to pristinamycin. the human campylobacter coli strains (except for two strains) behaved like the c. jejuni strains. | 1985 | 4091531 |
| evaluation of an enzyme-linked immunosorbent assay (elisa) for the detection of campylobacter jejuni antibodies, and comparison with a complement fixation test (cft). | an enzyme-linked immunosorbent assay (elisa) was developed for the detection of total anti-campylobacter immunoglobulins in human sera. in this assay disintegrated campylobacter bacteria were used as the antigen. absorption tests including other possibly enteropathogenic bacterial species showed that the elisa system displayed a high immunological specificity for campylobacter. using this elisa it was found that in about 80% of campylobacter patients these campylobacter antibodies are produced t ... | 1985 | 4091537 |
| comparison of enrichment broths for isolation of campylobacter jejuni. | growth of campylobacter jejuni was compared in enrichment broths of doyle and roman (appl. environ. microbiol. 43:1343-1353, 1982) and park et al. (c. e. park, z. k. stankiewicz, j. lovett, and j. hunt, can. j. microbiol. 27:841-842, 1981), as modified by lovett et al. (j. lovett, d. w. francis, and j. m. hunt, appl. environ. microbiol. 46:459-462, 1983). inoculated foods used were cream-pudding types, which may be cross-contaminated by improper handling, improper storage of meats prepared simul ... | 1985 | 4091559 |
| isolation of campylobacter jejuni from market chickens: effect of temperature and time of incubation. | during a 4-month period, starting in june 1983, campylobacter jejuni was isolated at 42% from a total of 115 samples of market chickens. the effects of the temperature and duration of incubation on the isolation of c. jejuni were evaluated. the samples were massaged in polyethylene plastic bags, filtered through cheesecloth, centrifuged, and the sediment was plated on a selective medium. isolation of c. jejuni was increased by incubating at 42 degrees c during 48 h as opposed to 35 degrees c for ... | 1985 | 4096141 |
| presence of zoonotic pathogens (yersinia spp., campylobacter jejuni, salmonella spp., and leptospira spp.) simultaneously in dogs and cats. | the purpose of this study was to determine the presence of zoonotic pathogens simultaneously in animals. the isolation of human pathogenic yersinia enterocolitica (ye), yersinia pseudotuberculosis (yp), campylobacter jejuni (cj), salmonella spp. (sal) and leptospira spp. (lep) in 318 cats and 252 dogs were performed in shimane prefecture, japan. a total of 13 isolates of yp (4 strains) and sal (9 strains) were recovered from intestine and/or mesenteric lymph nodes (mln) of 13 cats (4.1%) but not ... | 1985 | 4096152 |
| survey of plasmids and resistance factors in campylobacter jejuni and campylobacter coli. | a total of 688 isolates of campylobacter jejuni and campylobacter coli were screened for the presence of plasmid dna by agarose gel electrophoresis and were tested for susceptibility to ampicillin, chloramphenicol, erythromycin, streptomycin, and tetracycline. of the isolates examined, 32% were noted to harbor plasmid dna, ranging in size from 2.0 to 162 kilobases. only tetracycline resistance was noted to correlate with the presence of plasmids. plasmids capable of transferring tetracycline res ... | 1985 | 2984981 |
| dna relatedness and biochemical features of campylobacter spp. isolated in central and south australia. | investigations of the etiology of diarrhea in patients in south australia and the northern territory showed that campylobacter spp. other than campylobacter jejuni and c. coli were common in children. campylobacters which were hippurate positive, nitrate negative, and susceptible to cephalothin and polymyxins were shown to be closely related to c. jejuni by dna studies. thermotolerant catalase-negative campylobacters were also isolated. these were h2s negative and biochemically resembled the cat ... | 1985 | 2991331 |
| in vitro susceptibility of campylobacter jejuni to 27 antimicrobial agents and various combinations of beta-lactams with clavulanic acid or sulbactam. | the in vitro susceptibility of human isolates of campylobacter jejuni was investigated with 27 antibiotics and 8 combinations of beta-lactams with clavulanic acid or sulbactam. ansamycin, the new quinolines, erythromycin, and cefpirome were the most active drugs against c. jejuni; amoxicillin, ampicillin, cefotaxime, and ceftazidime 90% of the isolates, greater than or equal to 50 mg/liter). the activity of various beta-lactams was unchanged by the addition of clavulanic acid or sulbactam. | 1985 | 2994557 |
| diarrheal illness among infants and toddlers in day care centers. i. epidemiology and pathogens. | we conducted a 2-year prospective study of diarrheal illness in children ages 0 to 36 months in 22 day care centers in maricopa county, arizona. in 7464 child-months of observation, 465 sporadic cases and 170 outbreak-associated cases of diarrhea were identified. enteric pathogens were identified in 20% of diarrhea episodes. giardia lamblia, rotavirus, and campylobacter jejuni were the most common pathogens. giardia was significantly more common in toddlers than in infants and was found in 19% o ... | 1985 | 2995628 |
| [epidemiology, etiology and laboratory diagnosis of infectious diarrhea diseases in the tropics]. | diarrhoeal diseases belong to the leading causes of morbidity and mortality in tropical countries, especially in infants and small children. about one billion episodes are estimated for this group of age with 4.6 million fatalities. many causes are discussed to explain the high incidence: bottle feeding of infants, protein malnutrition, unsafe drinking water and unsafe disposal of excrements and sewage, unsufficient consciousness of personal and domestic hygiene, lack of knowledge on the origin ... | 1985 | 3000920 |
| susceptibility of campylobacter species to nalidixic acid, enoxacin, and other dna gyrase inhibitors. | nalidixic acid-resistant mutants of campylobacter jejuni and c. coli as well as "c. laridis" strains showed cross-resistance to another dna gyrase subunit a inhibitor, enoxacin (mic, 32 micrograms/ml), whereas c. fetus subsp. fetus, c. fetus subsp. venerealis, and "c. hyointestinalis" strains were all susceptible to enoxacin (mic, less than or equal to 2 micrograms/ml). all campylobacter species were resistant to novobiocin (mic, 32 to 512 micrograms/ml), but most strains were susceptible to the ... | 1985 | 3004325 |
| polymicrobial aetiology of travellers' diarrhoea. | of 35 us peace corps volunteers in thailand, 20 (57%) had a total of 30 episodes of diarrhoea during their first 6 weeks in the country. enteric pathogens were associated with 90% of the episodes. a single pathogen was identified in 17 (57%) episodes, 2-4 pathogens were identified in 10 (33%) episodes, and there were 15 symptomless infections. enterotoxigenic escherichia coli (etec) was identified in 37% of these episodes, and various salmonella serotypes were isolated in 33%. infections with 9 ... | 1985 | 2857430 |
| cytotoxic activity on chinese hamster ovary cells in culture filtrates of campylobacter jejuni/coli. | 1985 | 2863540 | |
| hospital epidemic of neonatal campylobacter jejuni infection. | 1985 | 2865631 | |
| restriction endonuclease digest patterns of chromosomal dna from nitrate-negative campylobacter jejuni-like organisms. | high molecular weight chromosomal dna was isolated from ten nitrate-negative campylobacters of human origin (gastric biopsies and faecal specimens). the dna was digested with various restriction endonucleases and the patterns obtained were compared with those of campylobacter coli, c. fetus subsp. fetus, c. jejuni and c. laridis reference strains. electrophoresis of hae iii digests of dna in 0.5% agarose gave excellent patterns which comprised at least 25 well-resolved bands with fragment sizes ... | 1985 | 2876913 |
| [yersinia enterocolitica and campylobacter jejuni in their significance for milk hygiene]. | 1985 | 2931637 | |
| the comparative in vitro activity of twelve 4-quinolone antimicrobials against enteric pathogens. | the minimal inhibitory concentrations (mics) of twelve 4-quinolone antimicrobials were determined for salmonella typhi (25), salmonella spp. (50), shigella spp. (50), campylobacter jejuni (100), vibrio cholerae (10), vibrio parahaemolyticus (10), yersinia enterocolitica (25), aeromonas hydrophila (25) and plesiomonas shigelloides (10). mics were determined using an agar dilution technique in mueller-hinton agar (oxoid, england) supplemented with 10% lysed horse blood. antibiotic containing plate ... | 1985 | 2941257 |
| [isolation of campylobacter fetus subsp. jejuni from feces and transport media during a diarrhea epidemic]. | 1985 | 3155666 | |
| in-vitro activity of pefloxacin compared to enoxacin, norfloxacin, gentamicin and new beta-lactams. | the in-vitro activity of pefloxacin was compared with that of norfloxacin, enoxacin, nalidixic acid, gentamicin, cefotaxime, ceftazidime and, where appropriate, other beta-lactams against a total of 363 recent clinical isolates. an agar dilution procedure was used to determine mics and two inocula (10(4) and 10(6) cfu) were used throughout. pefloxacin inhibited 90% of isolates of escherichia coli, klebsiella pneumoniae, proteus mirabilis, indole-positive proteus spp., enterobacter spp., shigella ... | 1985 | 3156112 |
| [digestive localizations of sexually transmitted diseases in male homosexuals]. | the common practice of sexual relations with many different and anonymous partners and the great variety of responsible micro-organisms account for the high incidence, growing complexity and uneasy prevention of sexually transmitted diseases of the digestive tract in male homosexuals. syphilis, gonorrhoea, papillomas, chancroid, donovanosis, herpes virus or chlamydia infections are known to be transmitted by anal coitus; amebiasis, giardiasis and shigellosis by oro-anal contact (faecal contamina ... | 1985 | 3160035 |
| vertical transmission of enteric pathogens at birth. | to determine how frequently mothers infect their infants with enteric pathogens at birth, stools were collected from 75 thai mothers immediately before delivery and from their infants 24-48 h later. enteropathogenic escherichia coli (epec) were isolated from 25 of the 75 mothers just prior to delivery (32%), enteroviruses from six (8%), enterotoxigenic e. coli from five (7%), and shigella, salmonella and vibrio parahaemolyticus each from one (1%). epec serotype 0128:k67 was isolated from one mot ... | 1985 | 2409892 |
| interaction of campylobacter jejuni and campylobacter coli with lectins and blood group antibodies. | lectins and blood group antibodies were used to probe the surface structures of campylobacter jejuni and campylobacter coli. of the 29 strains tested, there were distinct reaction patterns. the lectin-reactive and blood group antibody-reactive sites on the bacterial surface were distinguishable from the heat-stable (lipopolysaccharide) antigenic determinants. the interactions were strain specific. the reactive sites were stable with respect to culture media and passage and may be useful as addit ... | 1985 | 2410445 |
| basis for serological heterogeneity of thermostable antigens of campylobacter jejuni. | lipopolysaccharides (lps) were extracted from eight strains of campylobacter jejuni and purified by enzyme treatment to remove traces of rna, dna, and protein. this material was used to sensitize sheep erythrocytes for the passive hemagglutination assay that is presently used to serotype c. jejuni. the results confirmed that the thermostable antigen typing scheme is based on lps (o) antigens. the lps after sodium dodecyl sulfate-polyacrylamide gel electrophoresis and silver staining was found to ... | 1985 | 2412962 |
| antigenic analysis of campylobacter flagellar protein and other proteins. | outer membrane proteins of campylobacter jejuni and other campylobacter species were analyzed for their antigenic potentials by immunoblotting. polypeptides were resolved by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, transferred electrophoretically, and reacted with rabbit antisera to c. jejuni. each campylobacter species analyzed demonstrated a unique outer membrane protein antigenic profile; interspecies antigen sharing was observed to be compatible with the degree of dna relat ... | 1985 | 2578478 |
| western blot analysis of the human antibody response to campylobacter jejuni cellular antigens during gastrointestinal infection. | western blot analysis was used to identify antigenic components of campylobacter jejuni whole cells and outer membranes that elicit antibody responses in patients with campylobacter enteritis. acute- and convalescent-phase sera from eight patients were analyzed for antibody activity against their homologous infecting strains and heterologous clinical isolates. whole-cell and sarkosyl-insoluble membrane components were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and tra ... | 1985 | 2578479 |
| antigenic heterogeneity of lipopolysaccharides from campylobacter jejuni and campylobacter fetus. | the lipopolysaccharide (lps) structure of campylobacter spp. can be visualized with polyacrylamide gel electrophoresis by examining proteinase k-treated whole cell lysates. polyacrylamide gel electrophoresis lps profiles of c. jejuni strains are rough type with low concentrations of low-molecular-weight polysaccharide side chains, serum-resistant c. fetus strains have smooth-type lps, and serum-sensitive c. fetus strains have rough-type lps. we electroblotted the proteinase k-treated whole cell ... | 1985 | 2580793 |
| aetiological forms. | at least 25 etiologic agents have been implicated in acute diarrhea. in children, the most common are the rotaviruses, enterotoxigenic escherichia coli, campylobacter jejuni, salmonallae, and shigellae. for example, an attempt was made to isolate enteropathogenic micro-organisms in children under 2 years of age hospitalized in ethiopia with severe or moderate dehydration. a causal agent was identified in 70% of the 175 cases analyzed. 49% of these cases involved rotavirus infections, 19% inc ... | 1985 | 12314104 |
| egg penetration by campylobacter jejuni. | hens eggs were immersed in suspensions of campylobacter jejuni (4 strains) and examined for penetration and infection. penetration was demonstrated using an egg moulding technique and by culture. c. jejuni was recovered from shell membranes, but not from the albumin or yolk. embryonic deaths were not significantly greater in test than control eggs. unlike a strain of salmonella virchow, which was used as a control, the c. jejuni strains did not survive for more than 6 hours after penetration. it ... | 1985 | 18766924 |
| incidence of campylobacter spp. in broiler flocks monitored from hatching to slaughter. | the incidence of campylobacter jejuni/coli was examined in five flocks of broilers monitored from hatch to slaughter, in feed and water and in litter samples. a total of 1440 samples from 720 broilers was examined. campylobacter spp. were not isolated from broiler chicks at 1 day of age and were only isolated from one broiler chick in one flock at 1 week of age. in three flocks campylobacter spp. were isolated from all chicks sampled at 4 weeks of age. in the fourth flock all chicks sampled were ... | 1986 | 18766507 |
| prevalence of salmonella and campylobacter contamination in manitoba swine carcasses. | muscle samples from the neck and diaphragm and fecal samples were collected from five hogs from each of 50 lots of market hogs at three manitoba abattoirs. environmental samples were also collected. each sample was tested for salmonella contamination, and tissue and fecal samples were tested for campylobacter.salmonella was isolated from 34 (4.1%) of 821 samples, with 17 of the isolates coming from one lot of hogs and their concurrent environment. campylobacter was isolated from 175 (26.3%) of 6 ... | 1986 | 17422650 |
| anti-hla-b27 antibodies in sera from patients with gram-negative bacterial infections. | several forms of seronegative polyarthritis are strongly associated with hla-b27, and a number of microorganisms have been implicated in the etiology of these diseases. to explain the association between hla-b27 and arthritis initiated by infection with these organisms, it has been proposed that there is immunologic cross-reactivity between the b27 molecule and 1 or more microbial antigens, and that this cross-reactivity leads to tolerance to such infection and/or to the production of anti-hla-b ... | 1986 | 2421739 |
| a comparative study of the rod and coccoid forms of campylobacter jejuni atcc 29428. | coccoid forms in cultures of a strain of the enteric pathogen campylobacter jejuni were investigated. a culture containing 100% coccoid forms was non-viable. coccoid forms had a lesser content of cytoplasmic components and nucleic acids than rods of c. jejuni. during the conversion to coccoid forms nucleotides leaked from the cells. the results of treatments with ionic and non-ionic detergents, and lysozyme and ethylenediaminetetraacetic acid indicated a changed cell wall in coccoid forms compar ... | 1986 | 2422149 |
| monoclonal antibodies directed against the flagella of campylobacter jejuni: production, characterization and lack of effect on the colonization of infant mice. | eight monoclonal antibodies have been derived from balb/c mice hyperimmunized with the purified flagella from campylobacter jejuni strain 81116. these monoclonal antibodies are directed against flagella as demonstrated by reaction in elisa against flagellate and aflagellate antigens, radio-immunoprecipitation and electro-immunoblotting techniques. some of the antibodies react with a 60k minor protein as well as the 62k flagella protein. this protein may be related to an antigen expressed on the ... | 1986 | 2422256 |
| isolation and characterization of a common antigen in campylobacter jejuni and campylobacter coli. | flagellin protein was isolated and purified from two serotype reference strains of campylobacter jejuni, pen 1 and pen 3. each preparation was shown by sodium dodecyl sulfate-polyacrylamide gel electrophoresis to consist of a diffuse band with a molecular mass of approximately 62 kilodaltons. antisera were prepared against flagellin from pen 1, and specific antibody was isolated by affinity chromatography with flagellin protein covalently bound to cyanogen bromide-activated sepharose. the high-a ... | 1986 | 2424932 |
| common and specific epitopes of campylobacter flagellin recognized by monoclonal antibodies. | murine monoclonal antibodies to campylobacter jejuni recognized a flagellin epitope common to most campylobacter species and an epitope restricted to c. jejuni and c. coli. these epitopes are distinct from the serotype-specific epitope recently detected on the flagellin and have not been described previously. | 1986 | 2426201 |
| location of epitopes on campylobacter jejuni flagella. | flagella were isolated from strains of campylobacter jejuni belonging to different heat-labile serogroups and from a strain of campylobacter fetus, and sodium dodecyl sulfate-polyacrylamide gel electrophoresis showed that the flagellin molecular weights (mr) were approximately 62,000. the flagellins were cleaved by hydrolysis with cyanogen bromide, and sodium dodecyl sulfate-urea peptide gel electrophoresis showed that the c. jejuni flagellins were structurally similar, and differed from c. fetu ... | 1986 | 2430943 |
| ciprofloxacin in the treatment of acute bacterial diarrhea: a double blind study. | in a double-blind, randomized, placebo-controlled trial 50 adult patients with acute diarrhea received either 500 mg ciprofloxacin b.i.d. or a placebo for five days. results were evaluated in 21 patients in the ciprofloxacin group (10 with salmonella spp., 11 with campylobacter jejuni) and 25 patients in the placebo group (16 with salmonella spp., 5 with campylobacter jejuni, 4 with shigella spp.). the duration of fever in patients treated with ciprofloxacin was 1.5 days versus 2.3 days in the p ... | 1986 | 2941290 |
| influence of iron on growth, morphology, outer membrane protein composition, and synthesis of siderophores in campylobacter jejuni. | three human isolates of campylobacter jejuni were grown in a biphasic culture medium with and without the addition of a synthetic chelator to induce iron limitation. cells grown in low-iron medium exhibited slower growth rates and altered cellular morphology. increased numbers of longer, more filamentous forms were seen in gram-stained smears. three proteins, with apparent mrs of 82,000, 76,000, and 74,000, were consistently present in the outer membrane of cells grown in low-iron medium. at lea ... | 1986 | 2944843 |
| biochemical and serological characteristics of campylobacter jejuni strains isolated from chickens. | 1986 | 2953191 | |
| mucus colonization as a determinant of pathogenicity in intestinal infection by campylobacter jejuni: a mouse cecal model. | human isolates of the intestinal pathogen campylobacter jejuni have been shown to colonize mucus on the outer surface and deep within the intestinal crypts of gnotobiotic or germfree mice. the cecal crypts are preferentially colonized. a model of mucus colonization by c. jejuni in the mouse cecum has been developed, using antibiotic- and magnesium sulfate-treated specific-pathogen-free animals. these spiral-shaped bacteria colonize the mucus in a similar manner to the normal spiral-shaped microb ... | 1986 | 2935499 |
| [personal experience with the epidemiology of infections caused by campylobacter jejuni]. | 1986 | 2937545 | |
| in-vitro activity of newer quinolones against aerobic bacteria. | nalidixic and five newer 4-quinolones, ciprofloxacin, enoxacin, norfloxacin, ofloxacin and pefloxacin were tested against 576 recent clinical aerobic bacterial isolates. the 4-quinolones were regularly active (mic90 less than 4 mg/l) against the following bacteria: staphylococcus aureus, s. epidermidis, s. saprophyticus, different enterobacteriaceae, haemophilus influenzae, campylobacter jejuni, pseudomonas aeruginosa, agrobacter spp., aeromonas spp., plesiomonas spp., neisseria meningitidis. ot ... | 1986 | 2940214 |
| [campylobacter jejuni enteritis associated with hemolytic-uremic syndrome]. | 1986 | 2940534 | |
| lipopolysaccharide structures in enterobacteriaceae, pseudomonas aeruginosa, and vibrio cholerae are immunologically related to campylobacter spp. | to determine whether lipopolysaccharide (lps) structures of campylobacter species are immunologically related to those of 11 other gram-negative organisms, we immunoblotted from polyacrylamide gels the lps of these strains with immune rabbit serum raised against six campylobacter jejuni strains and two campylobacter fetus strains. the lps studied were from salmonella minnesota wild type and ra to re mutants, salmonella typhi, escherichia coli, yersinia enterocolitica, vibrio cholerae, and pseudo ... | 1986 | 3079730 |
| macrolide, lincosamide and streptogramin resistance in campylobacter jejuni/coli. | strains of erythromycin-resistant campylobacter jejuni/coli isolated in the united kingdom, canada and europe, were also resistant to tylosin, spiramycin and clindamycin and, like sensitive strains, were insensitive to virginiamycin and pristinamycin component b. these were designated as macrolide-lincosamide-streptogramin generalized resistant organisms and macrolide-lincosamide generalized resistant on the basis of patterns of resistance to pristinamycin components a and b. two erythromycin-se ... | 1986 | 3084440 |
| in vitro antibacterial activity of enoxacin (ci-919). | antibacterial activity of enoxacin was evaluated against more than 3,700 clinical isolates using the agar-dilution method and an inoculum of 10(4)-10(5) cells per site. for comparison other antibiotics appropriate for each species were also included. for most enterobacteria and for staphylococcus aureus, staphylococcus epidermidis and pseudomonas aeruginosa, the mic90 of enoxacin was below 2 mg/l. serratia marcescens was more resistant; the mic90 being 4 mg/ml. enoxacin also showed high activity ... | 1986 | 3093961 |
| [biotyping of campylobacter jejuni isolated from diarrhea patients]. | 1986 | 3095457 | |
| [prevalence of campylobacter jejuni complement fixing antibody in healthy people]. | 1986 | 3097197 | |
| [a food-borne infection caused by campylobacter jejuni serotype lauwers 19]. | report on a outbreak due to contaminated food involving 556 schoolchildren who got their lunch from a school kitchen. the outbreak occurred abruptly without any secondary infectious. examinations for salmonellae, shigellae, enteritis-coli, yersiniae and rotaviruses were negative. however, campylobacter jejuni were isolated from 105 specimens of faeces in rostock. sixty of these strains and another 2 that had been isolated at the same time but had no relation to the outbreak in rostock, were furt ... | 1986 | 3107256 |
| prevalence of various enteropathogens in the feces of diarrheic and healthy calves. | the presence of various enteropathogens was examined in the feces of homebred dairy calves reared in a restricted geographical area of france (north west of county of indre-et-loire) during winter 1983-1984. two distinct surveys were carried on: a case-control study including 32 diarrheic calves and 21 healthy calves bred in 53 different farms; and a separate study on nine diarrheic calves in another farm. the following infectious agents were looked for, by specific methods of detection: escheri ... | 1986 | 2877609 |
| substrate utilization by campylobacter jejuni and campylobacter coli. | an attempt was made to elucidate in campylobacter spp. some of the physiologic characteristics that are reflected in the kinetics of co2 formation from four 14c-labeled substrates. campylobacter jejuni and c. coli were grown in a biphasic medium, and highly motile spiral cells were harvested at 12 h. of the media evaluated for use in the metabolic tests, minimal essential medium without glutamine, diluted with an equal volume of potassium sodium phosphate buffer (ph 7.2), provided the greatest s ... | 1986 | 2877615 |
| identification and characterization of two campylobacter jejuni adhesins for cellular and mucous substrates. | campylobacter jejuni is able to colonize the human intestinal mucosa and cause disease. for this reason, it was important to investigate mechanisms by which c. jejuni adheres to epithelial cells and intestinal mucus gel. all strains of c. jejuni used were able to adhere to int 407 epithelial cells and mucus, but high adherence to one substrate did not necessarily indicate comparable adherence to the other. the adherence of c. jejuni to cells was inhibited partially by treating the bacterial cell ... | 1986 | 2873103 |
| nosocomial outbreak of campylobacter jejuni meningitis in newborn infants. | in a nosocomial outbreak of campylobacter jejuni infection 11 newborn infants (7 female, 4 male) had meningitis. the outbreak was caused by a single strain of c jejuni, as demonstrated by biotyping (biotype i), serotyping (lau 7/pen 18 on heat-stable antigens, a new serotype on heat-labile antigens), and the identical susceptibility pattern and outer-membrane-protein profile on sodium dodecyl sulphate/polyacrylamide gel electrophoresis. specific antibodies against the outbreak strain (enzyme-lin ... | 1986 | 2873408 |
| plasmid-mediated tetracycline resistance in campylobacter jejuni: expression in escherichia coli and identification of homology with streptococcal class m determinant. | plasmid pua466, a 45-kilobase transmissible tetracycline resistance plasmid from campylobacter jejuni was mapped with avai, avaii, bcli, hincii, psti, xhoi, and xbai. the resistance determinant was cloned and expressed in escherichia coli and was homologous with a class m determinant from streptococcus spp. | 1986 | 3005233 |
| enteropathogens associated with acute diarrhea in hospitalized infants. | thirty-five infants of low socioeconomic status who were living in urban santiago were hospitalized for acute diarrhea were prospectively evaluated for the presence of enteropathogens associated with the episode. some degree of malnutrition was evident in 20 infants (57.1%); 15 of these (75%) were under 6 months of age. mean duration of the hospital stay was 11.8 days for well-nourished patients and 15.7 days for the malnourished patients. one or more enteropathogens were found in 60% of the cas ... | 1986 | 3007718 |
| incidence of antibiotic resistance and characterization of plasmids in campylobacter jejuni strains isolated from clinical sources in alberta, canada. | antibiotic susceptibilities of 382 strains of campylobacter jejuni isolated in alberta, canada, in 1980 and 1981 were determined. although none were resistant to erythromycin or gentamicin, 5.4 and 22% of strains were resistant to ampicillin in 1980 and 1981, respectively. tetracycline resistance was noted in 6.8% of strains in 1980 and in 8.6% in 1981. moreover, resistance to high-level tetracycline (32-128 micrograms/ml) was always mediated by a plasmid of 45-50 kilobases. three transmissible ... | 1986 | 3008970 |
| pathogens that cause travelers' diarrhea in latin america and africa. | with the advent of rapid and convenient means of transportation, millions of persons travel each year from industrialized to developing countries in the tropics and subtropics. these travelers are at risk for a variety of infectious diseases that are endemic in these areas; the most frequently occurring of these is diarrhea. studies of groups of travelers to latin america and africa have found that approximately one-half develop diarrhea during their stay abroad. etiologic investigations of thes ... | 1986 | 3014633 |
| an epidemiological study of selected calf pathogens on holstein dairy farms in southwestern ontario. | fecal samples from calves on 78 randomly selected holstein dairy farms in southwestern ontario were screened for salmonella, campylobacter jejuni/coli, enteropathogenic escherichia coli, rotavirus and coronavirus. based on the observed prevalence, 22% of farms had calves infected with salmonella, 13% with campylobacter jejuni/coli, 41% with enteropathogenic e. coli, 19% with rotavirus and 5% with coronavirus. these estimates can be modified, using a method developed by mullen and prost (1983) fo ... | 1986 | 3017528 |
| cytotoxic and cytotonic factors produced by campylobacter jejuni, campylobacter coli, and campylobacter laridis. | complete toxigenicity studies were performed on 341 strains of campylobacter spp., including 23 nonhuman isolates. toxin profiles based on both cytotonic and cytotoxic factors were determined after analyzing responses in vero, hela, cho and y-1 cells. suckling mouse assays were consistently negative for all culture filtrates tested. toxin-producing strains were frequently encountered among both the human and nonhuman strains of campylobacter jejuni, c. coli, and c. laridis investigated. strains ... | 1986 | 3018039 |
| comparison between children treated at home and those requiring hospital admission for rotavirus and other enteric pathogens associated with acute diarrhea in melbourne, australia. | the etiology of acute diarrhea in children less than 42 months of age attending one pediatric hospital in melbourne, australia, was studied during a 7-month period encompassing the winter of 1984. pathogens identified in 157 children treated as outpatients with mild disease were compared with those in 232 children hospitalized with severe disease. the pathogens (and frequencies among outpatients and inpatients, respectively) detected were rotaviruses (32.5 and 50.9%), enteric adenoviruses (8.9 a ... | 1986 | 3020082 |
| genetic studies of kanamycin resistance in campylobacter jejuni. | campylobacter jejuni 3h40 and 4b20 harbored 59-kilobase (kb) self-transmissible plasmids encoding resistance to kanamycin and tetracycline. although the two antibiotic resistances were more frequently inherited together, some transconjugants and ethidium bromide segregants which were resistant to only one of these antibiotics were recovered. the kanamycin-susceptible, tetracycline-resistant segregants carried plasmids 4 kb smaller than the 59-kb plasmids of their parents, whereas the kanamycin-r ... | 1986 | 3021049 |
| approaches to the detection of enteric pathogens, including campylobacter, using nucleic acid hybridization. | a previous study of the detection of campylobacter jejuni in fecal samples spotted directly onto nitrocellulose filters before hybridization revealed a relatively low sensitivity and some false-positive results. we have investigated two factors that interfere with the detection of campylobacter jejuni in fecal samples: interfering substances that create false-positive background signals and nonspecificity of the probe. heterologous deoxyribonucleic acid probes bound nonspecifically to partially ... | 1986 | 3698541 |
| detection of pathogenic campylobacter species in blood culture systems. | because differences in recognition of campylobacter fetus and campylobacter jejuni in systemic infections may be due partially to differences in the ability to cultivate these organisms, we studied their growth characteristics in two widely used blood culture systems. in the roche septi-chek system (hoffman-la roche, inc., nutley, n.j.), over a broad range of inocula all strains were detected in broth within 2 days and on paddles within 3 days. in the bactec 6b aerobic bottles (johnston laborato ... | 1986 | 3700626 |
| the protection of infant mice from colonization with campylobacter jejuni by vaccination of the dams. | intraperitoneal vaccination of female mice, before mating, with a whole cell, heat-killed (62 degrees c) vaccine of campylobacter jejuni allowed the mother to confer immunity to her young, challenged orally 4-6 days after birth with the homologous strain. there was no protection against a strain of another serotype. heating the vaccine to 100 degrees c destroyed its protective properties. a vaccine prepared from an aflagellate variant of the original strain was as protective as the original vacc ... | 1986 | 3701036 |
| campylobacter jejuni in broilers: the role of vertical transmission. | the role of broiler eggs in the transmission of campylobacter jejuni to broiler grow-out flocks was investigated. six breeder flocks supplying broiler eggs to hatcheries were examined for cloacal carriage of c. jejuni. of 240 birds tested, 178 (74%) were c. jejuni-positive. eggs from these birds examined for c. jejuni penetration of the egg shell indicated that 185 of 187 were campylobacter-free. eggs from breeder flocks of unknown c. jejuni status were also examined for c. jejuni shell penetrat ... | 1986 | 3701037 |
| the cellular fatty acid composition of campylobacter species isolated from cases of enteritis in man and animals. | the cellular fatty acid composition of 41 strains of suspected campylobacter jejuni, 23 from human cases of gastroenteritis and 18 from animals, was examined by gas-liquid chromatography. three of the 23 human isolates and 2 of 18 animal isolates did not contain 19:0 cyclopropane fatty acid and were identified as c. laridis. the remaining 36 strains had cellular fatty acid profiles consistent with c. jejuni but could be divided into three groups on the ratio of the concentration of 18:1 and 19:0 ... | 1986 | 3701040 |
| pediatric gastroenteritis associated with aeromonas sobria and campylobacter jejuni. | 1986 | 3703054 | |
| ovine abortion associated with campylobacter jejuni. | 1986 | 3705362 | |
| persistence of multiply antibiotic-resistant campylobacter jejuni in a patient with the acquired immune deficiency syndrome. | chronic diarrhea due to campylobacter jejuni has been described in patients with hypogammaglobulinemia. a patient with the acquired immune deficiency syndrome (aids) and normal total serum immunoglobulins had persistent diarrhea and c. jejuni on stool culture for seven and a half months despite repeated antibiotic therapy. antibiotic sensitivity studies revealed the c. jejuni to be multiply antibiotic-resistant. evaluation of the mechanism of resistance showed the organism harbored a conjugative ... | 1986 | 3706381 |
| susceptibility of campylobacter jejuni to strain-specific bactericidal activity in sera of infected patients. | campylobacter jejuni is a common cause of inflammatory enteritis, which in normal hosts is usually self-limited and resolves without antibiotic therapy. c. jejuni bacteremia is very rare. we examined sera for bactericidal activity that might be important in limiting the extent of c. jejuni infection in man. we studied the ability of nonimmune sera and homologous and heterologous immune sera from infected patients to kill different fresh case isolates of c. jejuni in vitro. the reduction of the l ... | 1986 | 3710581 |