Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| lethal effect of recombinant human fas ligand in mice pretreated with propionibacterium acnes. | fas ligand (fasl) is a type ii membrane protein. binding of fasl to its receptor, fas, induces apoptosis. matrix metalloproteinase cleaves the membrane-bound human fasl to yield the active soluble form. here, we have produced a large amount of human soluble rfasl using the yeast, pichia pastoris. the purified rfasl was found to be glycosylated and to exist as a trimer. the rfasl was effective in inducing apoptosis in a fas-expressing t cell or a fibroblast cell line. the id50 of rfasl for mouse ... | 1997 | 9036978 |
| functional analysis of the fission yeast prp4 protein kinase involved in pre-mrna splicing and isolation of a putative mammalian homologue. | the prp4 gene of schizosaccharomyces pombe encodes a protein kinase. a physiological substrate is not yet known. a mutational analysis of prp4 revealed that the protein consists of a short n-terminal domain, containing several essential motifs, which is followed by the kinase catalytic domain comprising the c-terminus of the protein. overexpression of n-terminal mutations disturbs mitosis and produces elongated cells, using a pcr approach, we isolated a putative homologue of prp4 from human and ... | 1997 | 9102632 |
| molecular evolution of cytochrome c oxidase subunit iv: evidence for positive selection in simian primates. | cytochrome c oxidase (cox) is a multi-subunit enzyme complex that catalyzes the final step of electron transfer through the respiratory chain on the mitochondrial inner membrane. up to 13 subunits encoded by both the mitochondrial (subunits i, ii, and iii) and nuclear genomes occur in eukaryotic organisms ranging from yeast to human. previously, we observed a high number of amino acid replacements in the human cox iv subunit compared to mouse, rat, and cow orthologues. here we examined cox iv ev ... | 1997 | 9115172 |
| regulation of the myeloid-cell-expressed human gp91-phox gene as studied by transfer of yeast artificial chromosome clones into embryonic stem cells: suppression of a variegated cellular pattern of expression requires a full complement of distant cis elements. | identifying the full repertoire of cis elements required for gene expression in mammalian cells (or animals) is challenging, given the moderate sizes of many loci. to study how the human gp91-phox gene is expressed specifically in myeloid hematopoietic cells, we introduced yeast artificial chromosome (yac) clones and derivatives generated in yeast into mouse embryonic stem cells competent to differentiate to myeloid cells in vitro or into mouse chimeras. fully appropriate regulation was recapitu ... | 1997 | 9121478 |
| stat5 is a physiological substrate of the insulin receptor. | using the cytoplasmic domain of the insulin receptor (ir) in a yeast two-hybrid screen, we identified a cdna clone encoding the c-terminal 308 amino acids of human stat5b (stat5b-ct). stat5b-ct is tyrosine phosphorylated by purified ir kinase domain in vitro. insulin stimulates tyrosine phosphorylation of overexpressed stat5b-ct and endogenous stat5 in cells overexpressing ir. stat5 may be a direct target of the ir and, as a member of the stat family of transcription factors, may play a role in ... | 1997 | 9122188 |
| binary transgenic mouse model for studying the trans control of globin gene switching: evidence that gata-1 is an in vivo repressor of human epsilon gene expression. | to test whether human gata-1 (hgata-1) is involved in the transcriptional control of globin gene switching, we produced transgenic mice overexpressing hgata-1, crossed them with mice carrying a human beta-globin locus yeast artificial chromosome (beta yac), and analyzed globin gene expression in their progeny. mice carrying both the hgata-1 and the beta yac transgenes had normal levels of gamma- and beta-globin mrna and no distortion in the rate or in the timing of gamma-to-beta switch, indicati ... | 1997 | 9122214 |
| harakiri, a novel regulator of cell death, encodes a protein that activates apoptosis and interacts selectively with survival-promoting proteins bcl-2 and bcl-x(l). | programmed cell death is essential in organ development and tissue homeostasis and its deregulation is associated with the development of several diseases in mice and humans. the precise mechanisms that control cell death have not been elucidated fully, but it is well established that this form of cellular demise is regulated by a genetic program which is activated in the dying cell. here we report the identification, cloning and characterization of harakiri, a novel gene that regulates apoptosi ... | 1997 | 9130713 |
| a new family of 10 murine ovalbumin serpins includes two homologs of proteinase inhibitor 8 and two homologs of the granzyme b inhibitor (proteinase inhibitor 9). | serine proteinase inhibitors (serpins) are classically regulators of extracellular proteolysis, however, recent evidence suggests that some function intracellularly. such "ovalbumin" serpins include the human proteinase inhibitors 6 (pi-6), 8 (pi-8), and 9 (pi-9), plasminogen activator inhibitor 2, and the monocyte/neutrophil elastase inhibitor. pi-9 is a potent granzyme b (grab) inhibitor that has an unusual p1 glu and is present primarily in lymphocytes. in a search for the murine equivalent o ... | 1997 | 9182575 |
| hip-i: a huntingtin interacting protein isolated by the yeast two-hybrid system. | we report the discovery of the huntingtin interacting protein i (hip-i) which binds specifically to the n-terminus of human huntingtin, both in the two-hybrid screen and in in vitro binding experiments. for the interaction in vivo, a protein region downstream of the polyglutamine stretch in huntingtin is essential. the hip1 cdna isolated by the two-hybrid screen encodes a 55 kda fragment of a novel protein. using an affinity-purified polyclonal antibody raised against recombinant hip-i, a protei ... | 1997 | 9147654 |
| repression by ume6 involves recruitment of a complex containing sin3 corepressor and rpd3 histone deacetylase to target promoters. | sin3 and rpd3 negatively regulate a diverse set of yeast genes. a mouse sin3-related protein is a transcriptional corepressor, and a human rpd3 homolog is a histone deacetylase. here, we show that sin3 and rpd3 are specifically required for transcriptional repression by ume6, a dna-binding protein that regulates genes involved in meiosis. a short region of ume6 is sufficient to repress transcription, and this repression domain mediates a two-hybrid and physical interaction with sin3. coimmunopre ... | 1997 | 9150136 |
| cell cycle-dependent expression and spindle pole localization of a novel human protein kinase, aik, related to aurora of drosophila and yeast ipl1. | mutations in aurora of drosophila and related saccharomyces cerevisiae ipl1 kinase are known to cause abnormal chromosome segregation. we have isolated a cdna encoding a novel human protein kinase of 402 amino acids with a predicted molecular mass of 45.9 kda, which shares high amino acid identities with the aurora/ipl1 protein kinase family; hence the cdna is designated as aik (aurora/ipl1-related kinase). amino acid sequence of c-terminal kinase domain of aik shares 86, 86, 72, 59, and 49% ide ... | 1997 | 9153231 |
| isolation of a full-length human wnt7a gene implicated in limb development and cell transformation, and mapping to chromosome 3p25. | the wnt gene family has a role in development as well as tumourigenesis. one mouse member, wnt7a, is vital for limb development in vivo and also possesses transforming ability in vitro. this study reports the isolation of a full length of human homologue of mouse wnt7a gene by library screening. yeast artificial chromosome-fluorescence in situ hybridisation (yac-fish) mapped the wnt7a gene to chromosome 3p25. human wnt7a had an orf encoding a deduced protein of 349 aa that exhibited 97% and 92% ... | 1997 | 9161407 |
| the xpb subunit of repair/transcription factor tfiih directly interacts with sug1, a subunit of the 26s proteasome and putative transcription factor. | mutations in the basal transcription initiation/dna repair factor tfiih are responsible for three human disorders: xeroderma pigmentosum (xp), cockayne syndrome (cs) and trichothiodystrophy (ttd). the non-repair features of cs and ttd are thought to be due to a partial inactivation of the transcription function of the complex. to search for proteins whose interaction with tfiih subunits is disturbed by mutations in patients we used the yeast two-hybrid system and report the isolation of a novel ... | 1997 | 9173976 |
| severe reduction in leukocyte adhesion and monocyte extravasation in mice deficient in cc chemokine receptor 2. | cc chemokine receptor 2 (ccr2) is a prominent receptor for the monocyte chemoattractant protein (mcp) group of cc chemokines. mice generated by gene targeting to lack ccr2 exhibit normal leukocyte rolling but have a pronounced defect in mcp-1-induced leukocyte firm adhesion to microvascular endothelium and reduced leukocyte extravasation. constitutive macrophage trafficking into the peritoneal cavity was not significantly different between ccr2-deficient and wild-type mice. however, after intrap ... | 1997 | 9342361 |
| identification of n-acetylneuraminic acid and its 9-o-acetylated derivative on the cell surface of cryptococcus neoformans: influence on fungal phagocytosis. | sialic acids from sialoglycoconjugates present at the cell surface of cryptococcus neoformans yeast forms were analyzed by high-performance thin-layer chromatography, binding of influenza a and c virus strains, enzymatic treatment, and flow cytofluorimetry with fluorescein isothiocyanate-labeled lectins. c. neoformans yeast forms grown in a chemically defined medium contain n-acetylneuraminic acid and its 9-o-acetylated derivative. a density of 3 x 10(6) residues of sialic acid per cell was foun ... | 1997 | 9393779 |
| role of deoxyribonucleic acid polymerase epsilon in spermatogenesis in mice. | previous studies on dna polymerase epsilon indicate that this enzyme is involved in replication of chromosomal dna. in this study, we examined the expression of dna polymerases alpha, delta, and epsilon during mouse testis development and germ cell differentiation. the steady-state levels of mrnas encoding dna polymerase epsilon and the recombination enzyme rad51 remained constant during testis development, whereas the mrna levels of dna polymerases alpha and delta declined from birth until sexu ... | 1997 | 9408242 |
| yarrowia lipolytica srp54 homolog and translocation of kar2p. | to investigate the role of srp54p in protein translocation, the yarrowia lipolytica srp54 homolog was cloned. sequencing revealed an open reading frame of 536 amino acids coding for a 57.2 kilodalton polypeptide with 55 to 57% sequence identity to srp54ps of saccharomyces cerevisiae, schizosaccharomyces pombe, and mouse. like these srp54ps, y. lipolytica srp54p has an n-terminal domain with a highly conserved gtp-binding site and a methionine-rich c-terminal domain. differing results regarding t ... | 1997 | 9178502 |
| cell surface expression of two major yeast allergens in the pityrosporum genus. | we have previously identified two major allergens of pityrosporum orbiculare and characterized these as 37 kda and 67 kda proteins. | 1997 | 9179435 |
| a truncated form of rgs3 negatively regulates g protein-coupled receptor stimulation of adenylyl cyclase and phosphoinositide phospholipase c. | identification of a new family of proteins (rgs proteins) that function as negative regulators of g protein signaling has sparked new understanding of desensitization of this signaling process. recent studies with several mammalian rgs proteins has delineated their ability to interact with and function as gtpase-activating proteins specifically for g proteins in the gi family. here, we investigated the functional activity of rgs3 and a truncated form of rgs3 on g protein-coupled receptor-mediate ... | 1997 | 9182581 |
| clustering of missense mutations in the ataxia-telangiectasia gene in a sporadic t-cell leukaemia. | ataxia-telangiectasia (a-t) is a recessive multi-system disorder caused by mutations in the atm gene at 11q22-q23 (ref. 3). the risk of cancer, especially lymphoid neoplasias, is substantially elevated in a-t patients and has long been associated with chromosomal instability. by analysing tumour dna from patients with sporadic t-cell prolymphocytic leukaemia (t-pll), a rare clonal malignancy with similarities to a mature t-cell leukaemia seen in a-t, we demonstrate a high frequency of atm mutati ... | 1997 | 9288106 |
| nonfilamentous c. albicans mutants are avirulent. | candida albicans and saccharomyces cerevisiae switch from a yeast to a filamentous form. in saccharomyces, this switch is controlled by two regulatory proteins, ste12p and phd1p. single-mutant strains, ste12/ste12 or phd1/phd1, are partially defective, whereas the ste12/ste12 phd1/phd1 double mutant is completely defective in filamentous growth and is noninvasive. the equivalent cph1/cph1 efg1/efg1 double mutant in candida (cph1p is the ste12p homolog and efg1p is the phd1p homolog) is also defe ... | 1997 | 9298905 |
| cdna cloning and expression of murine 1-acyl-sn-glycerol-3-phosphate acyltransferase. | 1-acyl-sn-glycerol-3-phosphate (1-agp), also known as lysophosphatidic acid (lpa), is an intermediate of de novo biosynthesis of glycerophospholipids and triacylglycerol. lpa is also attracting much attention because of its growth stimulating effects. here we report cloning of murine cdna encoding 1-agp acyltransferase (1-agpat), which converts lpa into phosphatidic acid by incorporating acyl moiety at an-2 position. the cdna contains an open reading frame coding for 285 amino acids, with highly ... | 1997 | 9299423 |
| the mouse histone h1 genes: gene organization and differential regulation. | there are six mouse histone h1 genes present in the histone gene cluster on mouse chromosome 13. these genes encode five histone h1 variants expressed in somatic cells, h1a to h1e, and the testis-specific h1t histone. two of the genes that have not been assigned previously to the five somatic h1 subtypes have been identified as encoding the h1b and h1d subtypes. three of the h1 genes, h1a, h1c and h1t, are present on an 80 kb segment of dna that contains nine core histone genes. two others, h1d ... | 1997 | 9300059 |
| isolation and characterization of pbp, a protein that interacts with peroxisome proliferator-activated receptor. | in an attempt to identify cofactors that could possibly influence the transcriptional activity of peroxisome proliferator-activated receptors (ppars), we used a yeast two-hybrid system with gal4-ppargamma as bait to screen a mouse liver cdna library and have identified steroid receptor coactivator-1 (src-1) as a ppar transcriptional coactivator. we now report the isolation of a cdna encoding a 165-kda ppargamma-binding protein, designated pbp which also serves as a coactivator. pbp also binds to ... | 1997 | 9325263 |
| a gene spans the pseudoautosomal boundary in mice. | the x and y chromosomes of the mouse, like those of other mammals, are heteromorphic over most of their length, but at the distal ends of the chromosomes is a region of sequence identity, the pseudoautosomal region (par), where the chromosomes pair and recombine during male meiosis. the point at which the par diverges into x- and y-specific sequences is called the pseudoautosomal boundary. we have completed a genomic walk from the x-specific amelogenin gene to the par. analysis of this region re ... | 1997 | 9342357 |
| an eps homology (eh) domain protein that binds to the ral-gtpase target, ralbp1. | ral proteins constitute a family of small gtpases that can be activated by ras in cells. in the gtp-bound state, ral proteins bind to ralbp1, a gtpase-activating protein for cdc42 and rac gtpases. we have used the two-hybrid system in yeast to clone a cdna for a novel approximately 85-kda protein that can bind to an additional site on ralbp1. this newly identified protein contains an eps homology (eh) domain, which was first detected in the epidermal growth factor (egf) receptor substrate eps15. ... | 1997 | 9395447 |
| human sak related to the plk/polo family of cell cycle kinases shows high mrna expression in testis. | we identified the nucleotide sequence of a cdna encoding a polypeptide with a kinase domain that is related to the catalytic region of drosophila melanogaster polo, saccharomyces cerevisiae cdc5 as well as human fnk and plk. the novel gene seems to represent the human counterpart of the mouse gene sak. the sequence of sak predicts a serine/threonine kinase of 970 aa. the distribution of sak mrna in adult organs is restricted to certain tissues such as testis and thymus. northern analyses of tumo ... | 1997 | 21590086 |
| high-level expression of human interleukin-17 in the yeast pichia pastoris. | human interleukin-17 (hil-17) gene without the signal sequence was isolated from activated peripheral blood lymphocytes by rt-pcr, then highly expressed in the yeast pichia pastoris in the form of the glycosylated monomer. the monomer of rhil-17 stimulated mouse fibroblast 3t3 cells to secrete il-6 and was specifically bound to its receptors on 3t3 cells. | 1998 | 9891843 |
| characterization of mouse ubiquitin-like smt3a and smt3b cdnas and gene/pseudogenes. | mouse smt3a and smt3b cdnas encoding ubiquitin-like proteins of 110 and 95 amino acids, respectively, were isolated and sequenced. the sequence of the first 92 amino acids (ending with the conserved gly-gly) of mouse smt3a exhibited two differences at amino acid no. 38 and 76 in comparison with that of human smt3a. the c-terminal 18 amino acid sequence of mouse smt3a was completely different from the c-terminal 11 amino acid sequence of human smt3a. mouse and human smt3b were identical for a seq ... | 1998 | 9891849 |
| cdna cloning and characterization of mouse nifs-like protein, m-nfs1: mitochondrial localization of eukaryotic nifs-like proteins. | we have isolated a mouse cdna which shows significant sequence similarity to the yeast nifs-like gene (y-nfs1), and termed it m-nfs1. the deduced protein sequence (459 amino acids long) has several characteristic features common to those of bacterial nifs proteins, but distinct from them by its amino-terminal extension which contains a typical mitochondrial targeting presequence. m-nfs1 was found to be a soluble 47-kda protein in the matrix fraction of mouse liver mitochondria. the m-nfs1 gene w ... | 1998 | 9738949 |
| a novel nuclear receptor heterodimerization pathway mediated by orphan receptors tr2 and tr4. | a unique heterodimerization pathway involving orphan receptors tr2 and tr4 is demonstrated. tr2 and tr4 preferentially form heterodimers in solution as well as on dna elements containing a direct repeat-5 (dr5). the in vitro interaction between tr2 and tr4 is demonstrated by the yeast and the mammalian two-hybrid interaction assays, the pull-down assay, and the gel mobility shift assay. the in vivo interaction is demonstrated by following the intracellular localization of fusion receptors tagged ... | 1998 | 9737983 |
| a new large proteolytic complex distinct from the proteasome is present in the cytosol of fission yeast. | one eukaryotic proteolytic complex--the proteasome--is classed as the major nonlysosomal protease, by its known and suspected functions, its size and its complexity. it seems improbable that other enzymes may be capable of substituting, even partially, for the potent proteasome, as this complex has a vital role in many cellular processes. nevertheless, it is possible to adapt cultured el-4 mouse lymphoma cells to survive in the presence of a specific inhibitor of the proteasome. the inhibition o ... | 1998 | 9740805 |
| molecular evolution of hox gene regulation: cloning and transgenic analysis of the lamprey hoxq8 gene. | the mammalian hox clusters arose by duplication of a primordial cluster. the duplication of hox clusters created redundancy within cognate groups, allowing for change in function over time. the lamprey, petromyzon marinus, occupies an intermediate position within the chordates, both in terms of morphologic complexity and possibly cluster number. to determine the extent of divergence among hox genes after duplication events within vertebrates, we analyzed hox genes belonging to cognate group 8. h ... | 1998 | 9437854 |
| mutations in either nucleotide-binding site of p-glycoprotein (mdr3) prevent vanadate trapping of nucleotide at both sites. | vanadate trapping of nucleotide and site-directed mutagenesis were used to investigate the role of the two nucleotide-binding (nb) sites in the regulation of atp hydrolysis by p-glycoprotein (mouse mdr3). mdr3, tagged with a hexahistidine tail, was overexpressed in the yeast pichia pastoris and purified to about 90% homogeneity by ni-affinity chromatography. this protocol yielded purified, reconstituted mdr3 which exhibited high verapamil stimulation of atpase activity with a vmax of 4.2 micromo ... | 1998 | 9521779 |
| centromere protein b null mice are mitotically and meiotically normal but have lower body and testis weights. | cenp-b is a constitutive centromere dna-binding protein that is conserved in a number of mammalian species and in yeast. despite this conservation, earlier cytological and indirect experimental studies have provided conflicting evidence concerning the role of this protein in mitosis. the requirement of this protein in meiosis has also not previously been described. to resolve these uncertainties, we used targeted disruption of the cenpb gene in mouse to study the functional significance of this ... | 1998 | 9548711 |
| optimal activation of an endogenous gene by hox11 requires the nh2-terminal 50 amino acids. | the hox11 homeobox gene was first identified through studies of the t(7;10) and t(10;14) chromosomal translocations of acute t-cell leukemia. in addition, analysis of hox11-/- mice has demonstrated a critical role for this gene in murine spleen development. a possible mode of in vivo function for the hox11 protein in these two situations is regulation of target genes following dna binding via the homeodomain, but little is known about how hox11 regulates transcription in vivo. by performing tran ... | 1998 | 9584190 |
| excision repair of 8-hydroxyguanine in mammalian cells: the mouse ogg1 protein as a model. | 8-hydroxyguanine (8-oh-gua) is a major mutagenic lesion produced on dna by the oxidative stress induced by either the endogen metabolism or the exposure to external agents. in bacteria and yeast this modified base can be removed by specific dna glycosylases. recently a human gene coding for an 8-oh-gua dna glycosylase/ap lyase has been identified by its homology to the yeast ogg1. this gene is located in human chromosome 3p25, a region commonly rearranged in various cancers, specially in lung tu ... | 1998 | 10098454 |
| the mouse genome contains two expressed intronless retroposed pseudogenes for the sentrin/sumo-1/pic1 conjugating enzyme ubc9. | the ubiquitin conjugating (ubc) e2 enzyme ubc-9 conjugates the ubiquitin-like peptide sentrin/sumo-1/pic1 to target proteins which include the fas antigen. we show that the mouse genome contains four copies of the ubc-9 gene. these include a structural ubc-9 gene consisting of seven exons which encode a protein identical to human ubc-9, and three intronless processed pseudogenes. the open reading frames (orf) of two of the pseudogenes, ubc9-psi1 and ubc9-psi2, correspond to the cdna of ubc-9 and ... | 1998 | 10068040 |
| differentially expressed genes in c6.9 glioma cells during vitamin d-induced cell death program. | c6.9 rat glioma cells undergo a cell death program when exposed to 1, 25-dihydroxyvitamin d3 (1,25-d3). as a global analytical approach, we have investigated gene expression in c6.9 engaged in this cell death program using differential screening of a rat brain cdna library with probes derived from control and 1,25-d3-treated cells. using this methodology we report the isolation of 61 differentially expressed cdnas. forty-seven cdnas correspond to genes already characterized in rat cells or tissu ... | 1998 | 10200452 |
| interaction of mouse polycomb-group (pc-g) proteins enx1 and enx2 with eed: indication for separate pc-g complexes. | the polycomb group (pc-g) constitutes an important, functionally conserved group of proteins, required to stably maintain inactive homeobox genes repressed during development. drosophila extra sex combs (esc) and its mammalian homolog embryonic ectoderm development (eed) are special pc-g members, in that they are required early during development when pc-g repression is initiated, a process that is still poorly understood. to get insight in the molecular function of eed, we searched for eed-inte ... | 1998 | 9584197 |
| mechanism for benomyl action as a mitochondrial aldehyde dehydrogenase inhibitor in mice. | benomyl (a non-thio fungicide) inhibits hepatic mitochondrial low-km aldehyde dehydrogenase (maldh or aldh2) in ip-treated mice by 50% (ic50) at 7.0 mg/kg, which is surprisingly the same potency range as that for several dithiocarbamate fungicides (and the related alcohol abuse drug disulfiram) and thiocarbamate herbicides previously known for their alcohol-sensitizing action. the mechanism by which benomyl inhibits maldh was therefore examined, first by comparing the metabolism of benomyl with ... | 1998 | 9585485 |
| flow cytometric quantitation of yeast a novel technique for use in animal model work and in vitro immunologic assays. | animal models of fungal and other infectious diseases often require that the number of organisms in tissue be quantified, traditionally by grinding organs, plating them on agar and counting colony forming units (cfu). this method is labor intensive, slow as some fungi require two weeks of culture and limited in reliability by poor plating efficiency. to circumvent these problems, we developed a flow cytometric method to quantify yeast. in vitro cultured blastomyces dermatitidis, cryptococcus neo ... | 1998 | 9617831 |
| telomere elongation by hnrnp a1 and a derivative that interacts with telomeric repeats and telomerase. | telomeric dna of mammalian chromosomes consists of several kilobase-pairs of tandemly repeated sequences with a terminal 3' overhang in single-stranded form. maintaining the integrity of these repeats is essential for cell survival; telomere attrition is associated with chromosome instability and cell senescence, whereas stabilization of telomere length correlates with the immortalization of somatic cells. telomere elongation is carried out by telomerase, an rna-dependent dna polymerase which ad ... | 1998 | 9620782 |
| the capacity of polyomavirus enhancer binding protein 2alphab (aml1/cbfa2) to stimulate polyomavirus dna replication is related to its affinity for the nuclear matrix. | the nuclear matrix is thought to play an important role in the dna replication of eukaryotic cells, although direct evidence for such a role is still lacking. a nuclear matrix-associated transcription factor, polyomavirus (py) enhancer binding protein 2alphab1 (pebp2alphab1) (aml1/cbfa2), was found to stimulate py replication through its cognate binding site. the minimal replication activation domain (rad) was identified between amino acid (aa) 302 and aa 371 by using a fusion protein containing ... | 1998 | 9632801 |
| yac transgenic analysis reveals wilms' tumour 1 gene activity in the proliferating coelomic epithelium, developing diaphragm and limb. | wilms' tumour 1 gene (wt1) is required for the correct development of the urogenital system. to examine its regulation and expression, we created several transgenic mouse lines containing a beta-galactosidase reporter driven by the human wt1 promoter. a 5 kb promoter weakly recapitulated a subset of the endogenous wt1 expression pattern. in contrast, 470 and 280 kb yac transgenes reproduced the correct pattern with high activity and highlighted new expression sites. wt1 is expressed in the septu ... | 1998 | 10349631 |
| multiple gene duplication and expression of mouse bcl-2-related genes, a1. | here we report the genomic cloning and characterization of the murine a1 genes, which belong to the bcl-2 gene family. southern analysis indicated the existence of at least four a1 genes in the murine genome and four different a1 genes, designated a1-a, -b, -c and -d, were cloned from the murine genomic library. the a1-a, -b and -d genes consisted of two exons, whereas the a1-c gene contained 1 bp insertion in the coding region which may result in an aberrant and truncated protein by frame-shift ... | 1998 | 9645611 |
| mutations in the nucleotide-binding sites of p-glycoprotein that affect substrate specificity modulate substrate-induced adenosine triphosphatase activity. | the amino- and carboxy-terminal nucleotide-binding domains (nbd1 and nbd2) of p-glycoprotein (p-gp) share over 80% sequence identity. almost all of nbd1 can be exchanged by corresponding nbd2 segments with no significant loss of function, except for a small segment around the walker b motif. within this segment, we identified two sets of residues [erga --> dkgt (522-525) and t578c] that, when replaced by their nbd2 counterparts, cause dramatic alterations of the substrate specificity of the prot ... | 1998 | 9636053 |
| sequence analysis and expression of a novel mouse homolog of escherichia coli reca gene. | escherichia coli reca and its yeast homologs rad51 and dmc1 play crucial roles in mitotic and/or meiotic recombination and in repair of double-strand dna breaks. we have identified a murine novel reca-like gene (mmtrad). the predicted 329 amino acid protein showed significant homology to mouse rec2, rad51, dmc1 (or lim15) and e. coli reca. northern blot analysis revealed that mmtrad was ubiquitously transcribed in various tissues. | 1998 | 9655934 |
| functional reverse transcriptases encoded by a-type mouse line-1: defining the minimal domain by deletion analysis. | long interspersed elements, or lines, are retrotransposons that move via an rna intermediate. in mice, one polymorphic variant of l1 has amplified relatively recently, giving rise to the a-type subfamily in species belonging to the genus and subgenus mus. retrotransposition of line-1 (l1) requires the function of the l1-encoded reverse transcriptase that is produced from open reading frame 2 (orf2). here, we employ a convenient yeast genetic assay to determine the reverse transcriptase activity ... | 1998 | 9666081 |
| association of hsp105 with hsc70 in high molecular mass complexes in mouse fm3a cells. | the 105-kda stress proteins hsp105alpha and hsp105beta belong to a high molecular mass heat shock protein family which has been found in organisms from yeast to mammals. here we demonstrated the interaction of hsp105 with hsp70 family proteins in mouse fm3a cells. the association of hsp105 with hsc70 was shown by immunoprecipitation using anti-hsp105 antibody. furthermore, when cell extracts or partially purified hsp105 fractions from nonstressed or heat-shocked cells were analyzed by size exclu ... | 1998 | 9675148 |
| cells deleted for brca2 cooh terminus exhibit hypersensitivity to gamma-radiation and premature senescence. | the putative brca2-mmrad51 interaction is analyzed in mouse cells deleted for the cooh terminus of brca2 (amino acids 3140-3328), which contains a region that associates with mmrad51 by yeast two-hybrid. these cells are hypersensitive to gamma-radiation (suggesting defective recombinational repair) but not uv light (suggesting intact nucleotide excision repair) and maintain the g1-s and g2-m checkpoints after exposure to gamma-irradiation. cells deleted for the cooh terminus of brca2 progress th ... | 1998 | 9699678 |
| differential membrane localization and intermolecular associations of alpha-dystrobrevin isoforms in skeletal muscle. | alpha-dystrobrevin is both a dystrophin homologue and a component of the dystrophin protein complex. alternative splicing yields five forms, of which two predominate in skeletal muscle: full-length alpha-dystrobrevin-1 (84 kd), and cooh-terminal truncated alpha-dystrobrevin-2 (65 kd). using isoform-specific antibodies, we find that alpha-dystrobrevin-2 is localized on the sarcolemma and at the neuromuscular synapse, where, like dystrophin, it is most concentrated in the depths of the postjunctio ... | 1998 | 9732287 |
| thermodynamics and kinetics of the reaction of a single-chain antibody fragment (scfv) with the leucine zipper domain of transcription factor gcn4. | single-chain fv (scfv) fragments of antibodies have become important analytical and therapeutic tools in biology and medicine. the reaction of scfv fragments has not been well-characterized with respect to the energetics and kinetics of antigen binding. this paper describes the thermodynamic and kinetic behavior of the high-affinity scfv fragment sw1 directed against the dimeric leucine zipper domain of the yeast transcription factor gcn4. the scfv fragment was selected by the phage display tech ... | 1998 | 9737882 |
| identification and characterization of stk12/aik2: a human gene related to aurora of drosophila and yeast ipl1. | mutations in aurora of drosophila and related saccharomyces cerevisiae ipl1 protein kinases are known to cause abnormal chromosome segregation. we earlier isolated a cdna encoding a novel human protein kinase aik which shares high amino acid identity with the aurora/ipl1 protein kinase family. in the present study, a second human cdna highly homologous to aurora/ipl1 (aik2) was identified and the nucleotide sequence was determined (gene symbol stk12). the c-terminal kinase domain of the stk12 en ... | 1998 | 9858806 |
| assignment of galgt encoding beta-1, 4n-acetylgalactosaminyl-transferase (galnac-t) and kif5a encoding neuronal kinesin (d12s1889) to human chromosome band 12q13 by assignment to ici yac 26eg10 and in situ hybridization. medjph@stjames.leeds.ac.uk. | 1998 | 9858832 | |
| mint 3: a ubiquitous mint isoform that does not bind to munc18-1 or -2. | mint 1 and 2 are proteins that bind to munc18-1, an essential component of the synaptic vesicle fusion machinery, and are detectably expressed only in neurons [okamoto and südhof, j. biol. chem. 272, 31459-31464 (1997)]. mint 1 and 2 are composed of a variable n-terminal region that includes a conserved munc18-1-binding site, and a constant c-terminal region that contains one ptb and two pdz domains. we have now identified a third mint isoform, mint 3. similar to mint 1 and 2, the c-terminal hal ... | 1998 | 9860131 |
| isolation of growth suppressors from a cdna expression library. | we describe an experimental procedure for the isolation of growth inhibitory sequences from a complex cdna library. this approach first takes advantage of the setgap technique (selectable expression of transient growth arrest phenotype) to enrich for growth inhibitory sequences, followed by a screening procedure to identify individual cdnas that inhibit cell proliferation. here we provide a detailed description of the experimental protocol and report the characterization of two cdna sequences is ... | 1998 | 9872334 |
| characterization of the mouse src homology 3 domain gene sh3d2c on chr 7 demonstrates coexpression with huntingtin in the brain and identifies the processed pseudogene sh3d2c-ps1 on chr 2. | formation of intracellular protein complexes is often mediated by src homology 3 domain-containing proteins interacting with proline-rich target sequences on other proteins. the sh3d2c gene or its rat/human orthologs have been implicated in synaptic vesicle recycling due to interaction with dynamin i and synaptojanin in nerve terminals. in a yeast two-hybrid system, association with a huntingtin fragment containing an elongated stretch of polyglutamines was observed recently. by genetic mapping ... | 1998 | 9878254 |
| the enhancer of polycomb gene of drosophila encodes a chromatin protein conserved in yeast and mammals. | the polycomb group of genes in drosophila are homeotic switch gene regulators that maintain homeotic gene repression through a possible chromatin regulatory mechanism. the enhancer of polycomb (e(pc)) gene of drosophila is an unusual member of the polycomb group. most pcg genes have homeotic phenotypes and are required for repression of homeotic loci, but mutations in e(pc) exhibit no homeotic transformations and have only a very weak effect on expression of abd-b. however, mutations in e(pc) ar ... | 1998 | 9735366 |
| characterization of the myotubularin dual specificity phosphatase gene family from yeast to human. | x-linked myotubular myopathy (xlmtm) is a severe congenital muscle disorder due to mutations in the mtm1 gene. the corresponding protein, myotubularin, contains the consensus active site of tyrosine phosphatases (ptp) but otherwise shows no homology to other phosphatases. myotubularin is able to hydrolyze a synthetic analogue of tyrosine phosphate, in a reaction inhibited by orthovanadate, and was recently shown to act on both phosphotyrosine and phosphoserine. this gene is conserved down to yea ... | 1998 | 9736772 |
| bre: a modulator of tnf-alpha action. | a stress-responsive gene highly expressed in brain and reproductive organs (bre) is down-regulated after uv irradiation, dna damaging agents, or retinoic acid treatment. the human bre gene encodes a mrna of 1.9 kb, which gives rise to a protein of 383 amino acids with a molecular size of 44 kilodaltons. bre is not homologous to any known gene and its function has not been defined. here we report that bre was identified multiple times in a yeast two-hybrid screen of a murine cerebellar cdna libra ... | 1998 | 9737713 |
| dpm2 regulates biosynthesis of dolichol phosphate-mannose in mammalian cells: correct subcellular localization and stabilization of dpm1, and binding of dolichol phosphate. | biosynthesis of glycosylphosphatidylinositol and n-glycan precursor is dependent upon a mannosyl donor, dolichol phosphate-mannose (dpm). the thy-1negative class e mutant of mouse lymphoma and lec15 mutant chinese hamster ovary (cho) cells are incapable of dpm synthesis. the class e mutant is defective in the dpm1 gene which encodes a mammalian homologue of saccharomyces cerevisiae dpm1p that is a dpm synthase, whereas lec15 is a different mutant, indicating that mammalian dpm1 is not sufficient ... | 1998 | 9724629 |
| molecular cloning and functional characterization of murine sphingosine kinase. | sphingosine-1-phosphate (spp) is a novel lipid messenger that has dual function. intracellularly it regulates proliferation and survival, and extracellularly, it is a ligand for the g protein-coupled receptor edg-1. based on peptide sequences obtained from purified rat kidney sphingosine kinase, the enzyme that regulates spp levels, we report here the cloning, identification, and characterization of the first mammalian sphingosine kinases (murine sphk1a and sphk1b). sequence analysis indicates t ... | 1998 | 9726979 |
| hira, a mammalian homologue of saccharomyces cerevisiae transcriptional co-repressors, interacts with pax3. | hira maps to the digeorge/velocardiofacial syndrome critical region (dgcr) at 22q11 (refs 1,2) and encodes a wd40 repeat protein similar to yeast hir1p and hir2p. these transcriptional co-repressors regulate cell cycle-dependent histone gene transcription, possibly by remodelling local chromatin structure. we report an interaction between hira and the transcription factor pax3. pax3 haploinsufficiency results in the mouse splotch and human waardenburg syndrome (wsi and wsiii) phenotypes. mice ho ... | 1998 | 9731536 |
| the a gamma-globin 3' element provides no unique function(s) for human beta-globin locus gene regulation. | the human beta-globin locus is activated transcriptionally by a complex series of events that culminate in appropriate temporal and tissue-specific control over five separate genes during embryonic and early postnatal development. one cis-regulatory element in the locus, originally identified as an enhancer 3' to the agamma-globin gene, more recently has been suggested to harbor alternative or additional properties, including stage-specific silencer, insulator, nuclear matrix, or chromosome scaf ... | 1998 | 9707580 |
| the 5'-amp-activated protein kinase inhibits the transcriptional stimulation by glucose in liver cells, acting through the glucose response complex. | 5-amino-4-imidazolecarboxamide riboside (aicar) is known to stimulate rat liver 5'-amp-activated protein kinase (ampk). ampk is the mammalian homologue of snf1p in yeast, involved in derepression of glucose-repressed genes. we used aicar to test if ampk could also play a role in the regulation of glucose-dependent genes in mammalian cells. at a concentration which induces phosphorylation-dependent inactivation of hmg-coa reductase, aicar blocked glucose activation of three glucose responsive gen ... | 1998 | 9708898 |
| yeast coactivator mbf1 mediates gcn4-dependent transcriptional activation. | transcriptional coactivators play a crucial role in gene expression by communicating between regulatory factors and the basal transcription machinery. the coactivator multiprotein bridging factor 1 (mbf1) was originally identified as a bridging molecule that connects the drosophila nuclear receptor ftz-f1 and tata-binding protein (tbp). the mbf1 sequence is highly conserved across species from saccharomyces cerevisiae to human. here we provide evidence acquired in vitro and in vivo that yeast mb ... | 1998 | 9710580 |
| identification of a novel ring finger protein as a coregulator in steroid receptor-mediated gene transcription. | using the dna-binding domain of androgen receptor (ar) as a bait in a yeast two-hybrid screening, we have identified a small nuclear ring finger protein, termed snurf, that interacts with ar in a hormone-dependent fashion in both yeast and mammalian cells. physical interaction between ar and snurf was demonstrated by coimmunoprecipitation from cell extracts and by protein-protein affinity chromatography. rat snurf is a highly hydrophilic protein consisting of 194 amino acid residues and comprisi ... | 1998 | 9710597 |
| a 29-kilodalton golgi soluble n-ethylmaleimide-sensitive factor attachment protein receptor (vti1-rp2) implicated in protein trafficking in the secretory pathway. | expressed sequence tags coding for a potential snare (soluble n-ethylmaleimide-sensitive factor attachment protein receptor) were revealed during data base searches. the deduced amino acid sequence of the complete coding region predicts a 217-residue protein with a cooh-terminal hydrophobic membrane anchor. affinity-purified antibodies raised against the cytoplasmic region of this protein specifically detect a 29-kilodalton integral membrane protein enriched in the golgi membrane. indirect immun ... | 1998 | 9705316 |
| analysis of repair and mutagenesis of chromium-induced dna damage in yeast, mammalian cells, and transgenic mice. | chromium (cr) is a widespread environmental contaminant and a known human carcinogen. we have used shuttle vector systems in yeast, mammalian cells, and transgenic mice to characterize the mutational specificity and premutational dna damage induced by cr(vi) and its reduction intermediates in order to elucidate the mechanism by which cr induces mutations. in the yeast system, treatment of vector-containing cells with cr(vi) results in a dose-dependent increase in mutations in the sup4-o target g ... | 1998 | 9703488 |
| isolation of a gene encoding a novel member of the nuclear receptor superfamily from the critical region of usher syndrome type iia at 1q41. | 1998 | 9676434 | |
| nopp140 functions as a molecular link between the nucleolus and the coiled bodies. | coiled bodies are small nuclear organelles that are highly enriched in small nuclear rnas, and that have long been thought to be associated with the nucleolus. here we use mutational analysis, transient transfections, and the yeast two-hybrid system to show that the nucleolar phosphoprotein nopp140 functions as a molecular link between the two prominent nuclear organelles. exogenous nopp140 accumulated in the nucleolus rapidly, but only after a lag phase in coiled bodies, suggesting a pathway be ... | 1998 | 9679133 |
| molecular cloning and functional analysis of the arabidopsis thaliana dna ligase i homologue. | a cdna encoding the dna ligase i homologue has been isolated from arabidopsis thaliana using a degenerate pcr approach. the orf of this cdna encodes an amino acid sequence of 790 residues, representing a protein with a theoretical molecular mass of 87.8 kda and an isoelectric point (pi) of 8.20. alignment of the a. thaliana dna ligase protein sequence with the sequence of dna ligases from human (homo sapiens), murine (mus musculus), clawed toad (xenopus laevis) and the yeasts schizosaccharomyces ... | 1998 | 9681027 |
| characterization of kif1c, a new kinesin-like protein involved in vesicle transport from the golgi apparatus to the endoplasmic reticulum. | kinesins comprise a large family of microtubule-based motor proteins, of which individual members mediate specific types of motile processes. using the ezrin domain of the protein-tyrosine phosphatase ptpd1 as a bait in a yeast two-hybrid screen, we identified a new kinesin-like protein, kif1c. kif1c represents a member of the unc104 subfamily of kinesin-like proteins that are involved in the transport of mitochondria or synaptic vesicles in axons. like its homologues, the 1103-amino acid protei ... | 1998 | 9685376 |
| multiple functions of pmt1p-mediated protein o-mannosylation in the fungal pathogen candida albicans. | protein mannosylation by pmt proteins initiates o-glycosylation in fungi. we have identified the pmt1 gene and analyzed the function of pmt1p in the fungal human pathogen candida albicans. mutants defective in pmt1 alleles lacked pmt in vitro enzymatic activity, showed reduced growth rates, and tended to form cellular aggregates. in addition, multiple specific deficiencies not known in saccharomyces cerevisiae (including defective hyphal morphogenesis; supersensitivity to the antifungal agents h ... | 1998 | 9694829 |
| a retinoblastoma-binding protein that affects cell-cycle control and confers transforming ability. | the retinoblastoma (rb) gene is one of the most extensively studied tumour-suppressor genes. deletion or inactivation of both rb alleles is an essential, rate-limiting step in the formation of retinoblastoma and osteosarcoma that arise in families that carry mutant rb (ref. 2). rb inactivation is also found in other human tumours. whereas loss of rb function is associated with the loss of cellular proliferative control, introduction of a wild-type rb can suppress cell growth and tumorigenicity. ... | 1998 | 9697699 |
| anthrax lethal factor cleaves the n-terminus of mapkks and induces tyrosine/threonine phosphorylation of mapks in cultured macrophages. | lethal factor (lf) is the major virulence factor produced by bacillus anthracis. lf is sufficient to cause death in laboratory animals and cytolysis of peritoneal macrophages and macrophage cell lines. lf contains the characteristic zinc binding motif of metalloproteases and indirect evidence suggest that this hydrolytic activity is essential for its cytotoxicity. to identify the substrate(s) of lf, we have used the yeast two-hybrid system, employing a lf inactive mutant as bait. this approach h ... | 1998 | 9703991 |
| mitogen-activated protein kinase-defective candida albicans is avirulent in a novel model of localized murine candidiasis. | candida albicans strains with a deletion of the mitogen-activated protein kinase cek1 gene are defective in the yeast to hyphal transition on solid surfaces in vitro. the virulence of a cek1 delta/cek1 delta null mutant strain was compared with its wild-type parent strain (wt) in a novel model of localized candidiasis. the mammary glands of lactating mice (at day 5 postpartum) were infected for 2, 4 and 6 days with 50 microliter suspension containing 1 x 10(5), 1 x 10(6) and 1 x 10(7) blastopore ... | 1998 | 9741091 |
| identification of a novel cortactin sh3 domain-binding protein and its localization to growth cones of cultured neurons. | cortactin is an actin-binding protein that contains several potential signaling motifs including a src homology 3 (sh3) domain at the distal c terminus. translocation of cortactin to specific cortical actin structures and hyperphosphorylation of cortactin on tyrosine have been associated with the cortical cytoskeleton reorganization induced by a variety of cellular stimuli. the function of cortactin in these processes is largely unknown in part due to the lack of information about cellular bindi ... | 1998 | 9742101 |
| using yeast to study glucocorticoid receptor phosphorylation. | the glucocorticoid receptor (gr) is a phosphoprotein and a member of the steroid/thyroid receptor superfamily of ligand dependent transcription factors. when the glucocorticoid receptor is expressed in yeast (saccharomyces cerevisiae), it is competent for signal transduction and transcriptional regulation. we have studied the glucocorticoid receptor phosphorylation in yeast and demonstrated that the receptor is phosphorylated in both the absence and presence of hormone, on serine and threonine r ... | 1998 | 9749836 |
| identification and mutation analysis of a cochlear-expressed, zinc finger protein gene at the dfnb7/11 and dn hearing-loss loci on human chromosome 9q and mouse chromosome 19. | the dfnb7/11 locus for autosomal recessive non-syndromic hearing loss (arnshl) has been mapped to an approx. 1.5 mb interval on human chromosome 9q13-q21. we have determined the cdna sequence and genomic structure of a novel cochlear-expressed gene, znf216, that maps to the dfnb7/11 interval. the mouse orthologue of this gene maps to the murine dn (deafness) locus on mouse chromosome 19. the znf216 gene is highly conserved between human and mouse, and contains two regions that show homology to t ... | 1998 | 9758550 |
| the cytoplasmic zinc finger protein zpr1 accumulates in the nucleolus of proliferating cells. | the zinc finger protein zpr1 translocates from the cytoplasm to the nucleus after treatment of cells with mitogens. the function of nuclear zpr1 has not been defined. here we demonstrate that zpr1 accumulates in the nucleolus of proliferating cells. the role of zpr1 was examined using a gene disruption strategy. cells lacking zpr1 are not viable. biochemical analysis demonstrated that the loss of zpr1 caused disruption of nucleolar function, including preribosomal rna expression. these data esta ... | 1998 | 9763455 |
| infrequent mutations of the hogg1 gene, that is involved in the excision of 8-hydroxyguanine in damaged dna, in human gastric cancer. | dna glycosylase, encoded by the hogg1 gene, repairs 8-hydroxyguanine (oh8gua), which is an oxidatively damaged mutagenic base. to clarify whether the dna repair activity of hogg1 protein is involved in gastric carcinogenesis, we examined 9 gastric cancer cell lines and 35 primary gastric cancers for mutations and genetic polymorphisms of the hogg1 gene by polymerase chain reaction-single strand conformation polymorphism analysis. a g-to-a transition was detected in a gastric cancer cell line, mk ... | 1998 | 9765618 |
| molecular cloning and tissue-specific expression of mrad9, a murine orthologue of the schizosaccharomyces pombe rad9+ checkpoint control gene. | we have isolated a murine cdna, mrad9, that is orthologous to the fission yeast rad9+ and human hrad9 genes. mrad9 encodes a 389 amino acid long, 42,032 dalton protein that is 27% identical and 56% similar to rad9p, and 82% identical and 88% similar to hrad9, at the amino acid level. expression of the mrad9 cdna in schizosaccharomyces pombe rad9::ura4+ cells restores nearly wild-type levels of hydroxyurea resistance and early s phase checkpoint control to mutant fission yeast cell populations. h ... | 1998 | 9766521 |
| novel mutants of nab corepressors enhance activation by egr transactivators. | the ngfi-a binding corepressors nab1 and nab2 interact with a conserved domain (r1 domain) within the egr1/ngfi-a and egr2/krox20 transactivators, and repress the transcription of egr target promoters. using a novel adaptation of the yeast two-hybrid screen, we have identified several point mutations in nab corepressors that interfere with their ability to bind to the egr1 r1 domain. surprisingly, nab proteins bearing some of these mutations increased egr1 activity dramatically. the mechanism un ... | 1998 | 9774344 |
| nitric oxide synthase expression in macrophages of histoplasma capsulatum-infected mice is associated with splenocyte apoptosis and unresponsiveness. | splenic macrophages from histoplasma capsulatum-infected mice express inducible nitric oxide synthase (inos), and the inos expression correlates with severity of the infection. we examined whether production of no is responsible for apoptosis and the anti-lymphoproliferative response of splenocytes from mice infected with h. capsulatum. in situ terminal deoxynucleotidyl transferase nick end labeling revealed apoptotic nuclei in cryosections of spleen from infected but not normal mice. splenocyte ... | 1998 | 9784566 |
| identification of a novel highly conserved gene in the centromeric part of the major histocompatibility complex. | a novel highly conserved gene designated sacm2l alias are1) has been identified and fine mapped in the centromeric part of the major histocompatibility complex in rat, human, and mouse. sacm2l is closely linked to the ribosomal protein s18 gene rps18 with a distance of about 450 bp between the respective translational start points. numerous sacm2l-homologous est sequences can be identified in the database. northern blot experiments of rat sacm2l revealed a transcript of 3 kb in each organ tested ... | 1998 | 9790748 |
| cortactin associates with the cell-cell junction protein zo-1 in both drosophila and mouse. | cortactin is an actin filament-binding protein localizing at cortical regions of cells and a prominent substrate for src family protein-tyrosine kinases in response to multiple extracellular stimuli. human cortactin has been identified as a protein product of a putative oncogene, ems1. in this report, we describe the identification of a drosophila homolog of cortactin as a molecule that interacts with drosophila zo-1 using yeast two-hybrid screening. drosophila cortactin is a 559-amino acid prot ... | 1998 | 9792678 |
| adenovirus-mediated expression of a dominant negative mutant of p65/rela inhibits proinflammatory gene expression in endothelial cells without sensitizing to apoptosis. | we hypothesized that blocking the induction of proinflammatory genes associated with endothelial cell (ec) activation, by inhibiting the transcription factor nuclear factor kappab (nf-kappab), would prolong survival of vascularized xenografts. our previous studies have shown that inhibition of nf-kappab by adenovirus-mediated overexpression of i kappab alpha suppresses the induction of proinflammatory genes in ec. however, i kappab alpha sensitizes ec to tnf-alpha-mediated apoptosis, presumably ... | 1998 | 9794384 |
| yac rescue of downless locus mutations in mice. | mice with mutations at the downless (dl) locus have defects in hair follicle, tooth, sweat gland, preputial gland, meibomian gland, and tail development. the dl phenotype is analogous to the human genetic disorder termed autosomal hypohidrotic (or anhidrotic) ectodermal dysplasia (hed). on the basis of the identification of two related transgenic insertional mutations in the downless gene, yeast artificial chromosomes (yacs) were identified that map to the critical region of mouse chromosome (ch ... | 1998 | 9799834 |
| homology between human chromosome 2p13.3 and the wobbler critical region on mouse chromosome 11: comparative high-resolution mapping of sts and est loci on yac/bac contigs. | human chr 2p13-14 and homologous regions on mouse chrs 6 and 11 have been subjects of previous studies because they comprise the loci for several neuromuscular diseases. here we report on high-resolution mapping of 55 sts and est loci on human chr 2p13.3 and of 47 markers on the corresponding region on proximal mouse chr. 11. the maps comprise several known genes, meis1/meis1, rab1a/rab1a, mdh1/mor2, otx1/otx1, and rel on human 2p13.3 and mouse chr 11, respectively, as well as the wobbler (wr) c ... | 1998 | 9799840 |
| cloning and characterization of human sep1 (hsep1) gene and cytoplasmic localization of its product. | we isolated and sequenced a human cdna (designated as hsep1) encoding both a homologue of mouse dhm2 and budding yeast sep1. the gene was shown to be located on the long arm of chromosome 3 (3q25-26.1). the putative hsep1 product (hsep1p) consisted of 1694 amino acid residues with a molecular mass of about 190 kda. northern blot analysis showed a major 10-kb mrna expressed ubiquitously in various organs as well as a minor 5.5-kb mrna expressed relatively highly in the testis and placenta. hsep1p ... | 1998 | 9802570 |
| expression and regulation of mrna for putative fatty acid transport related proteins and fatty acyl coa synthase in murine epidermis and cultured human keratinocytes. | the epidermis has a requirement for fatty acids in order to synthesize cellular membranes and the extracellular lipid lamellar membranes in the stratum corneum. despite high endogenous production of fatty acids the transport of exogenous essential fatty acids into the epidermis is an absolute requirement. fatty acid uptake by keratinocytes has been shown to be mediated by a transport system. in this study we determined in murine epidermis and human cultured keratinocytes the expression of three ... | 1998 | 9804328 |
| high-resolution genetic, physical, and transcript map of the mnd2 region of mouse chromosome 6. | the autosomal recessive mutation mnd2 is responsible for a lethal neuromuscular wasting disorder in the mouse. a high-resolution genetic map of the mnd2 region of mouse chromosome 6 was generated by analysis of 1147 f2 offspring from an intersubspecific cross between strains c57bl/6j-mnd2/+ and cast/ei. the results localize mnd2 to the 0.2-cm interval between d6mit164 and d6mit128. a contig of overlapping yac, bac, and p1 clones spanning the nonrecombinant interval was constructed. one novel gen ... | 1998 | 9806835 |
| recently identified molecular aspects of intestinal iron absorption. | gene mapping techniques to identify gene mutations in humans and animals with phenotypic abnormalities in iron metabolism are providing important insights into the probable molecular mediators of intestinal iron absorption. positional gene cloning in humans with hereditary hemochromatosis has identified a mutation in a novel major histocompatibility complex (mhc) gene called hfe that is likely to be involved in regulating intestinal iron absorption. in addition, recent observations based on posi ... | 1998 | 9808632 |
| protein kinase profile of sperm and eggs: cloning and characterization of two novel testis-specific protein kinases (aie1, aie2) related to yeast and fly chromosome segregation regulators. | we have analyzed the general protein kinase expression profile in mouse sperm and eggs. a total of 41 different kinases were identified. in this study, we describe two novel protein kinases, designated aie1 (mouse) and aie2 (human), which share high amino acid identities with the serine/threonine (s/t) kinase domain of yeast ip11, fly aurora, and frog eg2. mutations in ip11 and aurora have been reported to cause abnormal chromosome segregation and centrosome separation. both aie1 and aie2 contai ... | 1998 | 9809744 |
| when overexpressed, a novel centrosomal protein, ranbpm, causes ectopic microtubule nucleation similar to gamma-tubulin. | a novel human protein with a molecular mass of 55 kd, designated ranbpm, was isolated with the two-hybrid method using ran as a bait. mouse and hamster ranbpm possessed a polypeptide identical to the human one. furthermore, saccharomyces cerevisiae was found to have a gene, ygl227w, the cooh-terminal half of which is 30% identical to ranbpm. anti-ranbpm antibodies revealed that ranbpm was localized within the centrosome throughout the cell cycle. overexpression of ranbpm produced multiple spots ... | 1998 | 9817760 |
| repression by the mad(mxi1)-sin3 complex. | the functions of myc in transformation and transactivation are countered by the suppressive actions of the mad(mxi1) family. mad(mxi1) proteins not only compete with myc for dimerization to max and binding to myc/max consensus sites but also recruit powerful repressors of gene expression. a prediction of the yin-yang relationship between myc and mad(mxi1) families would be that the latter constitutes a new class of tumor suppressors. here, we review the current literature on the mad(mxi1) family ... | 1998 | 9819568 |
| human carbon catabolite repressor protein (ccr4)-associative factor 1: cloning, expression and characterization of its interaction with the b-cell translocation protein btg1. | the human btg1 protein is thought to be a potential tumour suppressor because its overexpression inhibits nih 3t3 cell proliferation. however, little is known about how btg1 exerts its anti-proliferative activity. in this study, we used the yeast 'two-hybrid' system to screen for interacting protein partners and identified human carbon catabolite repressor protein (ccr4)-associative factor 1 (hcaf-1), a homologue of mouse caf-1 (mcaf-1) and saccharomyces cerevisiae ycaf-1/pop2. in vitro the hcaf ... | 1998 | 9820826 |
| recognition specificity of individual eh domains of mammals and yeast. | the eps homology (eh) domain is a recently described protein binding module that is found, in multiple or single copies, in several proteins in species as diverse as human and yeast. in this work, we have investigated the molecular details of recognition specificity mediated by this domain family by characterizing the peptide-binding preference of 11 different eh domains from mammal and yeast proteins. ten of the eleven eh domains could bind at least some peptides containing an asn-pro-phe (npf) ... | 1998 | 9822599 |