Publications

killing of virus-infected cells by cytotoxic lymphocytes.		1980	6249872
immunologic cytotoxicity against autologous human lymphocytes transformed or infected by epstein-bar virus: role of antibody-dependent cellular cytotoxicity in health individuals.	immunologic cytotoxicity against lymphocytes transformed or infected by epstein-barr virus (ebv) was mainly studied in an autologous in vitro system by 51cr release assay and ebv-determined nuclear antigen (ebna)-specific trypan blue exclusion method. when the cells of newly established ebv-transformed or spontaneously transformed lines were incubated with unfractionated autologous healthy donor lymphocytes or t-cell-depleted lymphocytes in the presence of ebv-positive autologous or allogeneic s ...	1980	6249949
ultrastructure of intracytoplasmic particles in a burkitt's lymphoma cell line.	electron microscopic examination of a burkitt's-lymphoma-derived cell line, p3hr-1, repeatedly revealed clusters of membrane-free nucleoids within the cytoplasmic matrix. the particles possessed an electron-lucent center surrounded by two layers - a dense ring and a fuzzy broad outer layer.	1980	6250988
synthesis of chromosomal proteins and epstein-barr virus dna in activated burkitt somatic cell hybrids.		1980	6251621
correlation between anti-rana and anti-ebna titers in normal subjects with and without hla-drw4.	we have determined the relationship of antibody titers to the rheumatoid arthritis nuclear antigen (rana) and of antibody titers to the epstein-barr nuclear antigen (ebna) to each other and to the drw4 b cell alloantigen in the sera of 34 normal white adults. by a sensitive indirect immunofluorescence (if) assay, 76% had rana antibody, compared to 23% by a micro-immunodiffusion assay. the correlation coefficient for the tube dilution titers of anti-rana and anti-ebna was 0.61 (p < 10(-4)). the 1 ...	1980	6251856
interaction of ebna with anti-ebna antibody and dna.	binding of epstein-barr-virus-determined nuclear antigen (ebna) to acid-fixed nuclei could be prevented by exposing ebna to anti-ebna antibodies. while ebna is eluted from acid-fixed nuclei by 0.4 m nacl, it was not eluted even by 1.5 m nacl if ebna bound to acid-fixed nuclei had been exposed to anti-ebna antibody. in this case the reaction was abolished, and ebna was at least partially eluted by 0.2 m glycine buffer, ph 2.8, or by 3 m nascn. ebna not exposed to antibody was completely eluted fr ...	1980	6252116
comparison of epstein-barr virus strains of different origin by analysis of the viral dnas.	epstein-barr virus (ebv) originating from burkitt's lymphoma (p3hr-1 and cc34-5), nasopharyngeal carcinoma (m-aba), transfusion mononucleosis (b95-8), and a patient with acute myeloblastic leukemia (qimr-wil) was isolated from virus-carrying lymphoid cell lines after induction with the tumor promoter 12-o-tetradecanoylphorbol-13-acetate. viral dna was analyzed by partial denaturation mapping and by use of the restriction endonucleases ecori, hindiii, and sali and separation of fragments in 0.4% ...	1980	6252328
[tumors caused by viruses].		1980	6252451
[cancer and virus].		1980	6253845
cloning and mapping of bamhi endonuclease fragments of dna from the transforming b95-8 strain of epstein-barr virus.	dna from the b95-8 strain of epstein-barr virus was cleaved into 29 different fragments by bamhi endonuclease (ec 3.1.23.6). all of the fragments except the terminal fragments have been inserted into the pbr322 cloning vector and replicated in escherichia coli. the location of each cloned dna fragment in the viral genome has been determined, providing a more detailed physical map of the genome than has been available previously.	1980	6253994
epstein-barr virus-transformed human lymphoblastoid cells for study of sister chromatid exchange and their evaluation as a test system.	epstein-barr virus-transformed human lymphoblastoid cell lines are suitable for detection of sister chromatid exchange (sce) induced by mutagens-carcinogens because they have shown a stable chromosome number and stable frequency of spontaneous sce for more than two years in culture. their spontaneous and induced sce frequencies were practically the same as those of phytohemagglutinin-stimulated lymphocytes from the same blood donors. the sce responses of one established cell line, nl3, to 13 typ ...	1980	6254652
[the diagnosis of nasopharyngeal carcinoma (author's transl)].	nasopharyngeal carcinoma (npc) is etiologically closely connected to the epstein barr virus. npc is the only carcinoma in man that can be diagnosed serologically. in the serum of patients with npc characteristic antibody titres of certain viral antigens can be demonstrated during the disease. conclusions regarding the success of treatment and on possible relapse can be deduced from these antibody titres. criteria of histological differention for npc prognosis are described, and the possible mode ...	1980	6254933
[acute burkitt-cell b type lymphoid leukemia].		1980	6255394
frequency and levels of antibodies to epstein-barr virus-specific dnase are elevated in patients with nasopharyngeal carcinoma.	sera from healthy individuals and patients with infectious mononucleosis, burkitt lymphoma, nasopharyngeal carcinoma, or other malignancies were examined for their capacity to neutralize epstein-barr virus (ebv)-induced dnase activity. sera were found that neutralized the ebv dnase but not herpes simplex virus type 1 or type 2 dnases, and vice versa. sera from 46 of the 49 patients with nasopharyngeal carcinoma examined (94%) neutralized > 6 units of ebv dnase per ml of serum. in contrast, only ...	1980	6255477
epidemiology of hodgkin's disease.		1980	6255608
[antineoplasm immunity testing in health workers at the orl department of the postgraduate medical institute].		1980	6256089
the role of viruses in human tumors.		1980	6257083
molecular theology, immunophilosophy, and autoimmune disease. ii. speculation on the aetiology and pathogenesis of rheumatoid arthritis.		1980	6264588
nasopharyngeal carcinoma: epidemiology, histopathology and aetiology.	the highest incidence of nasopharyngeal carcinoma (around 20 per 100,000 per year) is found in chinese populations in many countries. elevated rates (5--15) are found in eskimos, and in several racial groups in south-east asia. the incidence rates in malta and in some parts of northern africa are also moderately elevated. the incidence rates are low (less than 1) in virtually all other parts of the world. in singapore, the incidence rates are high in chinese (18.4), intermediate in malays (4.7) ...	1980	7212609
etiological characterization of hepatitis b surface antigen-negative hepatitis among adult patients in athens, greece.	in a 4-month period, 216 cases of acute viral hepatitis were diagnosed in adults at the infectious diseases hospital, athens, greece. twenty-six percent of these were hepatitis b surface antigen negative. a full set of clinical specimens was obtained from 19 of these patients, who were studied in depth for the etiology of their hepatitis. a total of 7 of the 19 patients had serological evidence of hepatitis a virus infection, and 2 had evidence of recent hepatitis b virus infection. the remainin ...	1980	6769949
prevalence of type b and non-a, non-b hepatitis in hemophilia: relationship to chronic liver disease.	to assess the relationship of liver dysfunction and hepatitis markers in hemophilic patients treated with factor viii or ix concentrates, we studied 103 patients with hemophilia a and b for 6-36 mo. elevated serum alanine aminotransferase was noted in 79% of the patients, with 51% of the patients showing persistent elevation for longer than 6 mo. thirteen patients (12%) were hbsag-positive, with 8 patients showing persistence of hbsag and abnormal serum alanine aminotransferase for more than 6 m ...	1980	6777232
anti-rana antibody: a marker for seronegative and seropositive rheumatoid arthritis.	without prior knowledge of the diagnosis or clinical status, sera from patients with various arthritides were screened for antibody against rheumatoid-arthritis-associated nuclear antigen (rana) with indirect immunofluorescence on cytocentrifuged raji cell preparations. 93% of 103 seropositive rheumatoid patients had anti-rana antibody, in contrast to 16% of 50 normal controls and a mean of 19% (10-29%) of 122 patients with other arthritides. anti-rana antibody was also demonstrated in 95% of 21 ...	1980	6102183
cyclosporin a immunosuppression, epstein-barr antibody, and lymphoma.		1980	6102249
anti-rana antibody and rheumatoid arthritis.		1980	6102704
who will get leukaemia?		1980	6103331
epstein barr virus nuclear antigen positive lymphoma after cyclosporin a treatment in patient with renal allograft.		1980	6104142
cyclosporin a and epstein-barr virus.		1980	6105533
binding of sv40 tumour antigen and epstein-barr-virus nuclear antigen to isolated acid-fixed nuclei.	sv40 tumour antigen (ta) bound to methanol-acetic-acid-fixed interphase nuclei and metaphase chromosomes. this reaction was visualized by the anti-complement immunofluorescence test. the reaction was negative when the nuclei had been treated with deoxyribonuclease prior to the addition of antigen, or when ta-antibody negative serum had been used. in parallel tests ta and epstein-barr virus nuclear antigen were examined. both the binding patterns and the optimum conditions for the reaction were s ...	1980	6107019
epstein-barr virus-induced lymphoblastoid cell lines from patients with primary immunodeficiency diseases.	peripheral lymphocytes from eight patients with congenital immunodeficiency diseases were infected with epstein-barr virus (ebv) in an attempt to establish b lymphoblastoid cell lines (lcl). these patients included three boys with congenital agammaglobulinaemia, two girls with hypogammaglobulinaemia, one boy with common variable immunodeficiency, one boy with severe combined immunodeficiency with adenosine deaminase deficiency, and one boy with digeorge syndrome. five of the patients bore no sur ...	1980	9537041
adult t-cell leukemia: antigen in an atl cell line and detection of antibodies to the antigen in human sera.	indirect immunofluorescence of certain human sera demonstrated an antigen(s) in the cytoplasm of 1--5% of the cells of a t-cell line, mt-1, from a patient with adult t-cell leukemia (atl), which is endemic in southwestern japan. the antigen was not detected in other human lymphoid cell lines, including six t-cell lines, seven b-cell lines, and four non-t non-b cell lines. the antigen did not show cross antigenicity with that of herpesviruses, including epstein--barr virus, herpes simplex virus, ...	1981	7031654
kawasaki disease and epstein-barr virus.		1981	6112339
treatment of childhood combined epstein-barr virus/cytomegalovirus infection with oral bovine transfer factor.	an illness lasting for two years, with recurrent fever, rash, abdominal pain, and arthralgia, developed in a four year old boy. he was found to have a combined epstein-barr virus and cytomegalovirus (cmv) infection. his symptoms, cmv in his urine, and an absent in vitro lymphocyte response to cmv antigen persisted for two years. after treatment with orally administered bovine transfer factor clinical symptoms and viruria disappeared and specific immunity to cmv developed. evaluation of this trea ...	1981	6113484
effects of hydrocortisone on the synthesis of epstein-barr virus antigens in p3hr-1 cells.	hydrocortisone was capable of enhancing the synthesis of epstein-barr virus antigens in producer p3hr-1 cells which had been induced with a 24-hr pulse of 5-iodo-2-deoxy-uridine. the effect of hydrocortisone was time dependent, suggesting that an early step in the induction process, and not the antigen synthesis itself, was influenced by the drug.	1981	6115566
manifold expression of new cellular genes in human lymphoid neoplasia.	previous studies performed in our laboratory have shown that a minor single-stranded dna component (ssdna) isolated from the bulk of double-stranded nuclear dna (ndna) of human, murine, or avian cells consisted mainly of active transcription sites. in the present work, ssdna isolated from human lymphoid cells, either malignant or not, amounted to 1.2-1.4% of the total ndna. this was labeled with 125i and utilized as a probe in rna-driven iterative hybridization and cross-hybridization assays. in ...	1981	6947241
human erythroid cell lines derived from a patient with acute erythremia.	three continuous human cell lines, designated kmoe, derived from a patient with acute erythremia (di guglielmo's disease) are reported. the cell lines are the cultures of (1) bone marrow cells, (2) peripheral blood cells, and (3) cells from a tumor developed into an athymic nude mouse after transplantation of the cultured bone marrow cells. cells of all three lines show morphology of immature erythroblast and have i(17q) marker chromosome. they are negative for both philadelphia chromosome and e ...	1981	6949905
cytogenetic studies on african burkitt's lymphoma cell lines: t(8;14), t(2;8) and t(8;22) translocations.	cytogenetic studies on ten african epstein-barr virus (ebv) positive burkitt's lymphoma (bl) cell lines were performed. the usual translocation t(8;14) (q24;q32) was found in five of them, a deletion del(8) (q24 leads to qter) in another one, while four variants were observed, two of these having a t(2;8) (p12;q24) translocation and two a t(8;22) (q24;q11) translocation. other chromosome abnormalities were seen in seven of the cell lines, but these varied from one cell line to another. thus, var ...	1981	7260888
detection of specific iga antibodies in serum of patients with varicella and zoster infections.	a sensitive solid-phase, enzyme-linked immunosorbent assay (elisa) was developed for detection of serum iga antibodies to varicella-zoster virus (vzv). the antigen consisted of a sonically disrupted extract of vzv-infected human embryo cells. rabbit antihuman iga peroxidase conjugate was used to detect human iga bound to viral antigens. in parallel, igm and igg antibodies to vzv were studied by elisa and by an immunoperoxidase antibody to membrane antigen technique, respectively. vzv iga antibod ...	1981	7298297
human post-thymic precursor cells in health and disease. iii. role of the autologous rosette-forming t cells in the generation of spontaneous killer cells in the autologous mixed lymphocyte reaction.	adult peripheral blood t lymphocytes activated in autologous mixed lymphocyte reactions (amlr) exerted cytotoxicity on both phytohaemagglutinin-stimulated cells and epstein-barr virus-transformed cells. when autologous rosette-forming t cells were removed from total t lymphocytes, there was no generation of cytotoxicity. re-addition of autologous rosette-forming t cells (tar cells) to a population of t cells depleted of tar cells restored the cytotoxic activity. treatment of tar cells with mitom ...	1981	6458877
early changes in the glycopeptides of human b-lymphocytes after epstein-barr virus infection in vitro.	human b lymphocytes obtained from healthy donors were infected with epstein-barr virus in vitro. from the initiation of infection to the final establishment of a permanent lymphoblastoid cell line, fucosyl glycopeptides of the cell surface were investigated. in order of appearance the following events took place: expression of epstein-barr virus-determined nuclear antigen, mitotic activity of the cells and specific glycopeptide alterations on the cell surface. this specific alteration in glycope ...	1981	6972922
ultraviolet inactivation of epstein--barr virus-induced nuclear antigen (ebna), dna and igm synthesis in human b lymphocytes.		1981	6264682
examination of epstein-barr virus and c-type proviral sequences in american and african lymphomas and derivative cell lines.	while epstein-barr viral (ebv) dna is nearly always detectable in african burkitt's lymphoma (bl), the relatively low frequency of bl in ebv-positive african children and the infrequent finding of ebv in american bl suggest that other cofactors may contribute to the malignant transformation. among these possible cofactors are type c oncornaviruses. to evaluate this possibility, we screened the cellular dna from 16 lymphomas (2 african, 14 american) and the dna from 20 lymphoma-derived cell lines ...	1981	6265077
[hepatitis caused by known viruses (other than a and b viruses)].		1981	6279919
[serological investigation of epstein-barr virus antibodies and heterophile antibodies of childhood in kumamoto prefecture (author's transl)].		1981	6279939
an ebv-associated, swedish case of burkitt-type malignant lymphoma.	morphological, serological, dna-homology and immunofluorescence studies of a rapidly fatal malignant lymphoma in a 25-year-old sweden male revealed most of the characteristics of an ebv-associated burkitt lymphoma. the various findings are discussed in relation to previously reported observations on endemic (african) and non-endemic burkitt lymphomas.	1981	6278823
[hepatitis in infectious mononucleosis].		1981	6280643
cyclosporin a abrogates proliferation of t cells and generation of suppressor and cytotoxic t-cell function induced by epstein-barr virus.	cyclosporin a (cya) promotes the outgrowth in vitro of epstein-barr-virus(ebv)-infected cells of immune donors. in the present study, the effects of cya on the t-cell responses developed to an in-vitro ebv infection were studied. cyclosporin a, by acting on the responder cells and not on stimulator cells, strongly inhibited the proliferation of t cells normally induced by ebv-infected autologous cells. moreover, t cells from cultures not exposed to cya exerted suppression on both alloantigen-ind ...	1981	6276295
epstein-barr virus susceptibility of b cells correlates to the presence of complement receptors but not surface immunoglobulins.	nuclear antigen (ebna) inducibility by epstein-barr virus (ebv) was assessed in relation to the presence of complement (c3) receptors and surface immunoglobulins (sig) on leukemic lymphocytes. when erythrocyte-antibody-complement complex(eac)-positive, sig-negative b cells were exposed to ebv, ebna synthesis occurred with a high frequency. in contrast, no significant ebna induction was evident in sig-positive, eac-negative b cells. cells possessing both markers were ebv-infectable, in proportion ...	1981	6276327
epstein-barr virus dna. x. direct repeat within the internal direct repeat of epstein-barr virus dna.	the 3,360-base-pair internal direct repeat (ir) in epstein-barr virus dna separates the short and long unique dna domains. ir has a single bamhi site. the juncture between the short unique domain and ir has been mapped by restriction endonucleases and is less than 2,600 nucleotides before the bamhi site in ir. the junction between ir and the long unique domain has been sequenced and is approximately 650 nucleotides after the bamhi site in ir. thus, relative to the start of ir at the juncture wit ...	1981	6275099
[eb virus receptor test].		1981	6275145
[seroepidemiology of epstein-barr virus].		1981	6275491
a sequential study of human b lymphocyte function from birth to two years of age.	the immunoglobulin synthesizing capacity of individual lymphocytes from newborns and infants aged 2, 6, 12 and 24 months respectively has been studied. the technique has been to expose purified lymphocytes to a t lymphocyte dependent (pokeweed mitogen) or a t lymphocyte independent (epstein-barr virus) activator of b lymphocytes. activation has been measured as immunoglobulin secretion of individual b lymphocytes using a hemolytic plaque assay. b lymphocytes from newborns can be made to synthesi ...	1981	6275657
[hepatitis caused by cytomegalovirus].	epidemical, clinical, biochemical, cytological and immunological aspects are studied in ten cases of hepatitis occurred endemically in a chirurgical ward between cancerous operated patients. three were hbsag positive. none of the others seven had clinical or serological dates enough to attribute them to a nor b virus hepatitis. the clinical and serological data exclude also epstein-barr virus or toxoplasma aethiology. nevertheless, the objective results of the study have entity enough to classif ...	1981	6278554
cloning of functional human t lymphocytes by limiting dilution: impact of filler cells and interleukin 2 sources on cloning efficiencies.	conditions for the cloning of alloactivated human lymphocytes by limiting dilution in the presence of interleukin 2 (il 2) and filler cells have been investigated. cloning efficiencies were extremely high (at least 40%) when il 2 derived from pooled peripheral blood mononuclear cells (pbm), stimulated with phytohaemagglutinin in the presence of irradiated epstein-barr virus-transformed lymphoid cell lines (lcl), was used in combination with irradiated pooled pbm as filler cells. cloning efficien ...	1981	6278580
estimation of b-cells transformed by epstein-barr virus in patients with congenital agammaglobulinemia.	in vitro immunoglobulin synthesis was measured in lymphocytes from four patients with congenital agammaglobulinemia (ca gamma) stimulated by two different polyclonal b-cell activators, pokeweed mitogen (pwm) and epstein-barr virus (ebv). in pwm-stimulated cultures, patient t-cells treated with mitomycin c (mmc) were able to help the immunoglobulin (ig) synthesis of normal b-cells. patient b-cell-enriched fraction not containing surface ig positive cells did not produce ig in combination with mmc ...	1981	6278674
epstein-barr virus, clinical symptomatology and immunodeficiency in non-hodgkin's lymphoma patients.		1981	6285121
the spectrum of epstein-barr virus disease.		1981	6283398
hla antigens in adults negative for antibody to epstein-barr virus (ebv).	evidence for a genetic component in susceptibility to epstein-barr virus (ebv) infection was sought by comparing hla a and b phenotypes of ebv antibody-negative scottish medical students and randomly chosen antibody-positive controls. no statistically significant differences were observed, but three antigens, (a10, a29 and b15), were relatively underrepresented in the ebv seronegative group; findings which agree with data previously reported from a similar study in los angeles. a strong associat ...	1981	6283690
[lymph node lesions caused by virus infections].		1981	6289546
eb-virus negative infectious mononucleosis.		1981	6309972
age-associated changes in epstein-barr virus-induced human lymphocyte autoantibody responses.	in the present investigation we have studied the capacity of the epstein-barr virus (ebv), a ubiquitous human b lymphocyte polyclonal activator, to induce cultured peripheral blood lymphocytes from umbilical cords, young adults (20 to 40 yr old), and elderly adults (75 to 90 yr old) to form igm antibodies to human igg or human thyroglobulin. the ebv preparation used was shown to exert its b cell stimulatory effect independently of t cell-suppressor effects. the cultures were studied in limiting ...	1981	6257787
fisher's syndrome associated with epstein-barr virus.		1981	6258542
[effect of age and sex on antibody formation].		1981	6258817
in vitro induction of hla-restricted cytotoxic t lymphocytes against autologous epstein-barr virus transformed b lymphoblastoid cell line.	cytotoxic t lymphocytes (ctl) against autologous ebv-transformed b lymphoblastoid cell line (lcl) were induced in vitro by culturing peripheral blood lymphocytes (pbl) of healthy donors together with mitomycin c-treated autologous lcl for 6 days. the cytotoxic cells developed only from the e-rosette-positive fraction but not from the negative fraction of pbl. these ctl killed autologous lcl but not pwm-stimulated autologous pbl. in addition, the ctl killed allogeneic lcl when at least 1 of the h ...	1981	6163815
interferon production by a human lymphoblastoid cell line (dg-75) free of the epstein-barr genome.	a new lymphoblastoid cell line, dg-75, was investigated for its ability to produce interferon. dg-75 cells, previously shown to be free of epstein-barr virus genome and receptors, could be grown in submerged culture and could produce interferon in titers comparable to interferon produced by namalva cells. the interferon produced was similar in size to the namalva interferon as determined by gel filtration in ultrogel aca54. the dg-75 cells present a new source of large quantities of interferon w ...	1981	6169305
iga antibody, antibody-dependent cellular cytotoxicity and prognosis in patients with nasopharyngeal carcinoma.	immunoglobulin fractions containing antibodies to the epstein-barr virus (ebv) were isolated from different sera and tested in the antibody-dependent cellular cytotoxicity (adcc) assay. all cytotoxic activity resided in the igg fraction. iga antibodies were not cytotoxic in this assay against cells expressing the ebv-induced membrane antigen complex. however, iga antibodies were able to block the igg-mediated adcc reaction, indicating that the iga and igg antibodies recognized the same evb-speci ...	1981	6169655
reactivation of epstein-barr virus-specific cytotoxic t cells by in vitro stimulation with the autologous lymphoblastoid cell line.	unfractionated mononuclear (um) cells and t cells freshly prepared from the blood of adult donors were co-cultivated in microtest plate wells with progressively lower numbers of cells from the autologous eb-virus-transformed b-cell line. the fresh cells present in co-cultures from eb virus antibody-negative (seronegative) donors regularly facilitated autologous cell line outgrowth, monitored after 4 weeks, whereas outgrowth was markedly inhibited in the corresponding co-cultures from seropositiv ...	1981	6169663
lysis of human b-lymphocyte-derived lymphoma/leukemia cells of established cell lines by interferon-activated natural killer (nk) cells.	cultured cell lines derived from malignant epstein-barr virus (ebv)-negative b cells, and representative of the more common types of naturally occurring non-hodgkin lymphomas and related leukemias, were found to be sensitive to lysis by human natural killer (nk) cells. the observed lysis of such cell lines was mediated by a population of interferon-augmentable, fcr-positive, non-adherent lymphoid cells, which were also able to kill the "standard" nk targets k562 and molt-4. when the nk susceptib ...	1981	6171529
collinear relationships of herpesvirus papio dna to epstein--barr virus dna.		1981	6259833
seroepidemiological study of relationships between epstein-barr virus and rheumatoid arthritis.	to elucidate the relationship between epstein-barr virus (ebv) and rheumatoid arthritis (ra), we measured antibodies to ra-associated nuclear antigen (anti-rana) and three other ebv-related antigens in the sera of ra patients and controls. our study groups consisted of 89 patients with definite or classical ra, mean age 56, male/female ratio 47:42; and 53 normal and osteoarthritis controls, mean age 51, male/female ratio 25:28. in addition to anti-rana, we measured antibodies to viral capsid ant ...	1981	6259207
very late relapses in patients with burkitt's lymphoma: clinical and serologic studies.	of 117 patients in remission for at least 12 months after chemotherapy for confirmed burkitt's lymphoma, 14 subsequently relapsed. frequency of ever relapsing in this group varied from 12% 2 years after chemotherapy to 3-8% 3-6 years after chemotherapy. risk of very late relapse (vlr) increased with the occurrence of meningeal disease and/or relapse before a remission of 12 months or more was achieved. the use of combination chemotherapy and especially prophylactic intrathecal methotrexate signi ...	1981	6259398
[leukocyte migration inhibition evoked by epstein barr virus antigen. i. immune response in healthy donors].		1981	6273776
burkitt's lymphoma in japan: clinicopathological features of twenty-two patients.	the clinicopathological features of 22 japanese patients with burkitt's lymphoma were reviewed. there were 14 males and 8 females with a mean age of 9.5 years. jaw tumors dominated the clinical picture at the time of diagnosis with abdominal tumors second in frequency. the central nervous system and bone marrow were involved during the course of the disease in 32% of the patients. the anti-epstein-barr (eb) virus antibody titers in japanese patients were comparable to those found in control grou ...	1981	6274060
epstein-barr virus-induced deoxynuclease and the reutilization of host-cell dna degradation products in viral dna replication.		1981	6274091
studies on the toxicity and binding kinetics of abrin in normal and epstein barr virus-transformed lymphocyte culture-i: experimental results - 3.	the effects of treatment with varying doses of abrin, a d-galactose binding lectin, on dna and protein synthesis of normal and epstein barr virus-transformed lymphocytes have been investigated. using data on ebv-transformed lymphocyte cell density as a function of time and dose of abrin, one can demonstrate that the mean number of sites bound/ebv-lymphocyte needed to exert a biological influence upon the cell dna synthesis lies between 59,264 and 370,000 sites/cell. using a simple packing model, ...	1981	6274718
some recent trends in studies of human lymphoid cells: b-cells, epstein-barr virus, and transformation.		1981	6274744
establishment of human cytotoxic t-cell lines specific for epstein-barr virus-transformed autologous cells.	ebv-specific cytotoxic t cells (tc) induced in vitro have continuously proliferated in vitro for over 9 months. the long-term maintenance of the tc growth was dependent on periodic supplementation of both irrediated ebv-transformed autologous lymphoblastoid cell line (lcl) cels and conditioned medium. the latter was derived from supernatants of human spleen-cell cultures stimulated with phytohemagglutinin. the cultured tc maintained significant cytotoxic activity to autologous lcl cells but not ...	1981	6274810
two small rnas encoded by epstein-barr virus and complexed with protein are precipitated by antibodies from patients with systemic lupus erythematosus.	primate cells harboring the epstein-barr virus (ebv) genome synthesize large amounts of two small rnas:eber 1 and eber 2 (ebv-encoded rna). these rnas are approximately 180 nucleotides long, possess 5' pppa termini, and lack poly(a). they have different t1 and pancreatic rnase digestion fingerprints. they are not found in normal b lymphocytes, in transformed b lymphocytes that lack ebv dna, in t lymphocytes transformed by herpesvirus ateles, or in a variety of other nonlymphoid mammalian cells. ...	1981	6262773
induction of replication of epstein-barr virus dna by 12-o-tetradecanoyl-phorbol-13-acetate. ii. inhibition by retinoic acid and 9-(2-hydroxyethoxymethyl) guanine.		1981	6263008
a defect in the formation of uropod-bearing lymphocytes (hand-mirror cells) in patients with the wiskott-aldrich syndrome.	the formation of a uropod by lymphocytes (hand-mirror cells) represents a morphologic stage of immune activation and motility in lymphocytes. immune complexes have been previously shown to induce hand-mirror-cell (hmc) formation in human lymphocytes and have been associated with increased number of hmc in acute leukemia. we studied the ability of immune complexes to induce hmc in patients with wiskott-aldrich syndrome (was), a disease of cellular immune deficiency. our findings indicate that lym ...	1981	6263088
malignant b-cell lymphoma following and associated with infectious mononucleosis. a comparison of two cases.	the present report describes two young males with clinically diagnosed infectious mononucleosis (im) who subsequently were diagnosed as having malignant b-cell lymphoma (i.e., immunoblastic sarcoma of b-cells). despite these apparent similarities, there were fundamental differences between the two cases. the first patient, who lymphoma was diagnosed 9 months after im, was one of a well-described kindred with the x-linked lymphoproliferative syndrome (xlp) in which affected young males lack the a ...	1981	6263125
antibodies to epstein-barr virus, herpes simplex type 1, cytomegalovirus and measles virus in psychiatric patients.	distribution of antibodies to herpes simplex type 1 (hsv1), epstein-barr virus (ebv), cytomegalovirus (cmv) and measles virus (mv) was studied in sera and cerebrospinal fluids (csf) of 41 patients with schizophrenia, 27 patients with primary affective disorders and 25 control patients with neurological diseases. no significant differences in distribution and mean geometric titers (gmt) of antibodies to hsv1 between the psychiatric and control groups were found. distribution and gmt of antibodies ...	1981	6263228
genome of a mononucleosis epstein-barr virus contains dna fragments previously regarded to be unique to burkitt's lymphoma isolates.	we wished to learn whether the genomes of strains of emb isolated from patients with infectious mononucleosis are consistently distinguishable from those of strains from burkitt's lymphoma. the genome of a new transforming strains (ff41) of ebv isolated from saliva of a patient with uncomplicated infectious mononucleosis was compared with the dna of b95-8, the only other available virus from mononucleosis. it had been found previously that b95-8 has a deletion of about 8 md in the region of the ...	1981	6263500
pathophysiologic analysis of peripheral blood lymphocytes from patients with primary immunodeficiency. i. ig synthesis by peripheral blood lymphocytes stimulated with either pokeweed mitogen or epstein-barr virus in vitro.	in the present study 5 patients with common variable hypogammaglobulinemia (cvh) and 4 patients with selective iga deficiency (iga-d) were analyzed for the cellular defects responsible for impaired ig synthesis with use of peripheral blood lymphocytes stimulated with either pwm or ebv in vitro. by the use of co-culture with pwm, all the patients examined had intrinsic b cell defects restricted to the synthesis of ig class corresponding to the low or absent ig class(es) in the sera. two types of ...	1981	6263975
sera of patients with hairy cell leukemia immunoprecipitate ebv-related antigens.		1981	6264236
embryonic erythroid differentiation in the human leukemic cell line k562.	k562 human leukemia cells synthesize embryonic hemoglobins after culture in the presence of hemin. we have rigorously identified these hemoglobins by globin chain analysis and peptide mapping. no adult hemoglobin could be detected, and beta-globin synthesis was less than 2 ppm of total protein synthesis. persistent embryonic globin gene expression is known to occur as a consequence of globin gene deletions. however, restriction endonuclease mapping showed that the globin gene complexes in k562 c ...	1981	6264439
ebv infection of mitogen-stimulated human b lymphocytes.	b lymphocytes from human blood were treated with the mitogen protein a (staph, aureus), which induces dna synthesis in, as well as differeniation of, the b lymphocytes. the cells were subsequently exposed to epstein-barr virus (ebv). ebv-infected and mitogen-stimulated cells were detected simultaneously, by using a combination of immunofluorescence (for ebna) and autoradiography (for dna-synthesis). in the initial phase (1-2 days) after addition of the mitogen, stimulated cells were as susceptib ...	1981	6270012
immunofluorescent staining of nuclear antigen in lymphoid cells transformed by herpesvirus papio (hvp).	an improved fixation method for antigen detection in lymphoblastoid cells is described. herpesvirus papio nuclear antigen (hupna) could be stained in several transformed lymphoid cell lines by anti-complement immunofluorescence (acif). antibody to hupna was detected in many human sera containing antibodies to epstein-barr virus capsid and nuclear antigen (ebna). rheumatoid arthritis sera showed a high incidence of both anti-ebna and anti-hupna antibodies.	1981	6270210
the spontaneous and induced synthesis of epstein-barr virus antigens in raji cells immobilized on surface coated with anti-lymphocyte globulin.	immobilization of raji cells on surface coated with anti-lymphocyte globulin (alg) at low cell densities lead to the synthesis of epstein-barr virus (ebv) early antigen (ea) in up to 5% of the cells. at higher cell densities the percentage of antigen-positive cells decreased and at confluency no antigen synthesis was observed. addition of iododeoxyuridine (idurd) to low density cultures increased the expression of ea to 20%, whereas in confluent cultures the cells could not be induced to synthes ...	1981	6270256
epstein-barr virus-induced proteins. iv. characterization of an ebv-associated phosphopolypeptide.	cells of the epstein--barr virus (ebv) non-producing lines nc37 and raji were induced by the tumour promoter tpa and were labelled with 32p. the analysis by immunoprecipitation with human vca+ea+ sera revealed that a major phosphopolypeptide with a mol. wt. of 58 000 was specifically precipitated. in addition, a phosphopolypeptide of the same size was the dominant phosphopolypeptide detected in ebv-producing b95-8 and p3hr-1 cells as well as in p3hr-1-superinfected nc37 cells. this phosphopolype ...	1981	6270267
sequence complexity of circular epstein-bar virus dna in transformed cells.	a simplified procedure, based on several methods previously used to isolate circular dna molecules from bacteria, was derived for the preparation of covalently closed circular viral dna molecules from large quantities of lymphocytes transformed by epstein-barr virus. the protocol can be applied both to virus nonproducer lines and to lines containing cells activated to virus production. sufficient amounts o highly purified viral dna of intracellular origin were obtained from b95-8 and raji cells ...	1981	6270367
defective ebv-specific suppressor t-cell function in rheumatoid arthritis.	several lines of evidence, including high antibody titers to epstein-barr virus (ebv)-associated antigens and rapid transformation of b cells into lymphoblastoid cells lines, suggest an association between ebv and rheumatoid arthritis. when lymphocytes from normal immune donors were infected with ebv in culture, they produced an exponentially increasing number of immunoglobulin-secreting cells for eight to 10 days. thereafter, there was a marked late suppression of their response, mediated by im ...	1981	6270556
establishment and characterization of permanent cell lines from patients with acute and hairy cell leukemia.	four cell lines were established from peripheral blood of patients with leukemia. all lines express the epstein--barr virus nuclear antigen (ebna) and therefore should be classified as lymphoblastoid cell lines. however, one of the lines uhkt-5 established from a patient with acute myelomonocytic leukemia has some features not typical for lymphoblastoid cell lines. the cells resemble to macrophages with the phagocytic ability for yeasts, strong alpha-naphthylacetate esterase positivity in phagoc ...	1981	6270579
analysis of restriction endonuclease fragments of intracellular epstein-barr virus dna and isoenzymes indicate a common origin of the raji, nc-37, and f-265 human lymphoid cell lines.		1981	6270894
variant translocation in a non endemic case of burkitt's lymphoma: t (8;22) in an epstein--barr virus negative tumour and in a derived cell line.		1981	6271555
impaired regulation of epstein-barr virus-induced lymphocyte proliferation in rheumatoid arthritis is due to a t cell defect.	the rate of outgrowth of ebv-infected b lymphocytes is regulated by normal t lymphocytes. removal of t cells from normal whole lymphoid populations (pbm) markedly shortens the outgrowth time of the remaining b lymphocytes. there is little difference in the much more rapid outgrowth of rheumatoid pbm after the removal of t cells, which suggests that ra lymphoid cells are unable to regulate this process. to determine whether ra t cells are defective, or ebv-infected ra b cells are unresponsive to ...	1981	6271870
on the dynamics of abrin binding to receptor sites in normal and epstein barr virus transformed lymphocyte cell cultures.	the effects of treatment with varying doses of abrin, a d-galactose binding lectin, on dna an protein synthesis of normal and epstein barr virus transformed lymphocytes has been investigated. activation, stimulation, and relative toxicity factor indices are studied, as well as possible relationships between dna and protein synthesis rates, as measured by simultaneous tritiated thymidine (3h-tdr) and 14c-leucine uptake. studies of the two new indices, the metabolic self and cross coupling indices ...	1981	6271962
analysis of the transformation of human lymphocytes by epstein-barr virus iii. induction of early events by p3hr-1 strain without subsequent immortalization.	t-cell depleted human cord blood lymphocytes were exposed to p3hr-1 strain of epstein-barr virus (ebv) and simultaneous observations of immunofluorescence, cellular morphology, and autoradiography were carried out in individual cells. soon after infection, nuclear antigen (ebna) synthesis, blastogenesis, and dna synthesis occurred, as was previously observed b95-8 strain ebv infection. although mitosis followed with characteristic cell aggregate formation, the cell proliferation was temporary an ...	1981	6272076
retinoid-induced growth inhibition of herpesvirus-transformed marmoset lymphoblastoid cell lines.	the in vitro proliferation of herpesvirus-transformed marmoset lymphoblastoid cell lines (lcl) was inhibited by retinoic acid and retinyl acetate. both herpesvirus-ateles-and herpesvirus-saimiri-transformed lcl with t-cell characteristics and epstein-barr-transformed lcl with b-cell markers were more sensitive to retinoic acid than to retinyl acetate. inhibition of lcl proliferation was dependent on retinoid concentration and became apparent only after 3-4 days of exposure. in vitro assays for m ...	1981	6272171
a new view of the etiology of nasopharyngeal carcinoma.		1981	6272265
serological follow-up of children with infectious mononucleosis caused by epstein-barr virus.	ninety children, most under 6 years of age, 73 of whom were suffering from a disease suspect of infectious mononucleosis, were examined serologically for ebv infection. acute ebv infection was demonstrated in 40 cases. these children were followed up until the end of the second year by examining serum samples taken every month of every third month for anti-vca-igm, anti-vca-igg, anti-vca-iga anti-ebna, and for the specific antibodies to the components d and r of the early antigen. heterophil ant ...	1981	6272540