Publications

cdna and deduced amino acid sequence of a mouse dna repair enzyme (apex nuclease) with significant homology to escherichia coli exonuclease iii.	we purified a mouse dna repair enzyme having apurinic/apyrimidinic endonuclease, dna 3'-phosphatase, 3'-5'-exonuclease and dna 3' repair diesterase activities, and designated the enzyme as apex nuclease. a cdna clone for the enzyme was isolated from a mouse spleen cdna library using probes of degenerate oligonucleotides deduced from the n-terminal amino acid sequence of the enzyme. the complete nucleotide sequence of the cdna (1.3 kilobases) was determined. northern hybridization using this cdna ...	1991	1939131
molecular cloning and expression of a mouse muscle cdna encoding adenylosuccinate synthetase.	adenylosuccinate synthetase (ec 6.3.4.4) catalyzes the first step in formation of amp from imp. at least two isozymes exist in vertebrate tissue. an acidic form, present in most tissues, has been suggested to be involved in de novo biosynthesis while a basic isozyme, which predominates in muscle, appears to function in the purine nucleotide cycle. antibodies specific for the basic isozyme detect a single protein in mouse tissues with highest levels in skeletal muscle, tongue, esophagus, and hear ...	1991	1939273
gene expression of the a- and b-chain of mouse c1q in different tissues and the characterization of the recombinant a-chain.	immunoscreening of a mouse macrophage cdna library with an anti-mouse c1q-antibody resulted in the isolation of cdna clones. the deduced amino acid sequence was homologous to the a-chain of human c1q. homology on the dna level was found to be 76% and on the protein level 72% thus it appeared the clones coded for the mouse c1q a-chain. an immunoblot of murine serum c1q separated by sds-page was detected with an a-chain specific antibody that had been affinity purified on recombinant mouse c1q a-c ...	1991	1940381
characteristics of alpha-hemolytic strains of escherichia coli isolated from dogs with gastroenteritis.	twenty-four hemolysin producing (hly+) strains of escherichia coli isolated from dogs with gastroenteritis were investigated for their virulence markers and their phenotypic properties. the strains were distributed over eleven known e. coli o-serogroups and most of them were heterogeneous for their phenotypes. all strains were found to produce alpha-hemolysin which was detected by southern hybridization and colony immunoblotting using a specific gene probe and a monoclonal antibody. eight strain ...	1991	1949555
integration of pharmacokinetics and pharmacodynamics of imipenem in a human-adapted mouse model.	the relationship between the pharmacokinetics and bactericidal activity of imipenem against pseudomonas aeruginosa and escherichia coli was investigated in a neutropenic mouse thigh infection model. to circumvent the problem of short elimination time in small animals, imipenem was administered in fractionized, decreasing doses such that the pharmacokinetic profiles as observed in humans after intravenous and intramuscular injections were approximated in mice. the human-simulated kinetic profile ...	1991	1952865
catalytic antibodies: contributions from engineering and expression in escherichia coli.	antibodies have been raised against the transition state of many reactions and shown to catalyse the relevant reaction. their moderate catalytic efficiencies can be increased by protein engineering, if ways can be found to express the engineered antibody. we have developed a system by which fully functional fv and fab fragments can be expressed in escherichia coli. the fv fragment dissociates at low concentrations; we therefore devised methods to stabilize the fragment. we showed that the fv fra ...	1991	1959443
functional analysis of an oxygen-regulated transcriptional enhancer lying 3' to the mouse erythropoietin gene.	erythropoietin, the major hormone controlling red-cell production, is regulated in part through oxygen-dependent changes in the rate of transcription of its gene. using transient transfection in hepg2 cells, we have defined a dna sequence, located 120 base pairs 3' to the poly(a)-addition site of the mouse erythropoietin gene, that confers oxygen-regulated expression on a variety of heterologous promoters. the sequence has the typical features of a eukaryotic enhancer. approximately 70 base pair ...	1991	1961720
systemic delivery of human growth hormone by injection of genetically engineered myoblasts.	a recombinant gene encoding human growth hormone (hgh) was stably introduced into cultured myoblasts with a retroviral vector. after injection of genetically engineered myoblasts into mouse muscle, hgh could be detected in serum for 3 months. the fate of injected myoblasts was assessed by coinfecting the cells with two retroviral vectors, one encoding hgh and the other encoding beta-galactosidase from escherichia coli. these results provide evidence that myoblasts, which can fuse into preexistin ...	1991	1962213
disulfide assignments in recombinant mouse and human interleukin 4.	the disulfide pairings of mouse and human interleukin 4 (il-4) proteins have been determined. the purified proteins, synthesized by recombinant dna technology, are fully active as judged by their ability to stimulate an appropriate biological response in a variety of functional assays. peptide maps were produced by digesting the proteins with pepsin and separating the resulting fragments by reverse-phase hplc using linear acetonitrile-tfa gradients. cystine-containing peptides were identified by ...	1991	1993171
the presence of sialidase-sensitive sialosylgangliotetraosyl ceramide (gm1b) in stimulated murine macrophages. deficiency of gm1b in escherichia coli-activated macrophages from the c3h/hej mouse.	the stimulated murine macrophage was found to contain 11 major gangliosides of which 8 were determined to be monosialylated. the thin-layer chromatographic patterns were complicated by the presence of both sialic acid and ceramide fatty acid heterogeneity. n-glycolyl and n-acetylneuraminic acid-containing species were present for each ganglioside characterized. although c18 sphingosine was the only long chain base detected, ceramide fatty acid ranged from c16 to c24 carbon moieties. based on gas ...	1991	2005385
the c terminus of mouse ornithine decarboxylase confers rapid degradation on dihydrofolate reductase. support for the pest hypothesis.	several years ago, we proposed that polypeptide regions rich in proline (p), glutamic acid (e), serine (s), and threonine (t) (pest) target intracellular proteins for destruction (rogers, s., wells, r., and rechsteiner, m. (1986) science 234, 364-368). to test the pest hypothesis, we have produced chimeric proteins in which the n or c terminus of mouse dihydrofolate reductase is extended by the pest-containing c terminus of mouse ornithine decarboxylase. oligonucleotides encoding the 37 c-termin ...	1991	2040628
comparative efficacies of ticarcillin, ticarcillin/clavulanic acid, piperacillin and cefoxitin against polymicrobial infections in mice caused by escherichia coli and bacteroides fragilis.	a model of localised abscess formation was used to establish mixed infections caused by escherichia coli and bacteroides fragilis. the beta-lactamase producing, ticarcillin-resistant strains e. coli e96 and b. fragilis vpi 8708 were used to produce one infection, and in another infection, a beta-lactamase hyperproducing strain e. coli 41548 was combined with a ticarcillin-susceptible strain, b. fragilis b3. treatment, at doses producing clinically achievable concentrations in mouse serum, began ...	1991	2050417
a chimeric mammalian transactivator based on the lac repressor that is regulated by temperature and isopropyl beta-d-thiogalactopyranoside.	lap267 is a laci activator protein (lap) containing an insertion of the transcriptional activation domain of the herpes simplex virus virion protein 16 within the inducer-binding and dimerization domain of the lac repressor protein. lap267 strongly induces expression in a conditional manner from a minimal simian virus 40 early promoter linked to lac operator sequences. lap267 is temperature-sensitive, activating expression at 32 degrees c but not at 39.5 degrees c. it is allosterically regulated ...	1991	2052587
influence of the oral administration of excess copper on the immune response.	we have studied the influence of the oral administration of excess copper (cu) on the immune response. with this aim, mice maintained on standard laboratory diet received 50, 100, 200, or 300 ppm of cu as copper sulfate in the drinking water during 3 to 10 weeks. inhibition of the proliferative response to concanavalin a was observed in mice exposed to 100 ppm of cu for 8 weeks and to 200 ppm of cu for either 3 or 8 weeks. conversely, a significant increase in the proliferative response to esche ...	1991	2055356
comparison of biologic activities of synthetic lipopentapeptide analogs of bacterial lipoprotein in mice.	mitogenicity, lethal toxicity, induction of tumor necrotizing factor (tnf), and antitumor activity against meth a fibrosarcoma of four chemically synthesized lipopentapeptide analogs, s-[2,3-bis(palmitoyloxy)-2r (designated as kab-1), -2s(kab-3)-propyl]-n-palmitoyl-(r)-cysteinyl-(s)-seryl- (s)-seryl-(s)-asparaginyl-(s)-alanine, s-[2,3-bis(palmitoyloxy)-2r(kab-2), and -2s(kab-4)-propyl]-n-[(2,2,2)-trichloroethoxycarbonyl]-(r)- cysteinyl-(s)-seryl-(s)-seryl-(s)-asparaginyl-(s)-alanine, of bacteria ...	1991	2069759
bacterial adhesion to mucosal surfaces.	bacteria utilize one or more of several mechanisms to adhere to mucosal surfaces. members of the enterobacteriaceae and related bacteria typically elaborate adhesive filamentous appendages from their surfaces termed fimbriae (or pili). vibrio cholerae expresses at least three types of these structures that are likely involved in various stages of colonizing the intestine. so far one of these, termed tcp for toxin coregulated pilus, has been shown to be essential for colonization and virulence in ...	1991	12041763
histopathologic-microbiologic correlates of invasiveness in a mouse model of ascending unobstructed urinary tract infection.	to clarify the usefulness of histopathology in evaluating invasiveness during acute cystitis and pyelonephritis in a mouse model of urinary tract infection, findings from bladder and kidney sections of mice inoculated transurethrally with escherichia coli were compared with results of bladder, kidney, spleen, and blood cultures and with changes in peripheral blood leukocyte counts. all of the 14 bladder histopathologic abnormalities evaluated were significantly associated with a positive bladder ...	1992	1730896
a comparative study of specific gene probes and standard bioassays to identify diarrhoeagenic escherichia coli in paediatric patients with diarrhoea in bangladesh.	we compared the usefulness of gene probes with standard bioassays to identify diarrhoeagenic escherichia coli amongst isolates from bangladeshi children under 1 year of age with diarrhoea. e. coli isolates were analysed with specific gene probes for localised adhesiveness (la), diffuse adhesiveness (da), heat-labile toxin (lt), heat-stable toxin (st), shiga-like toxins (slt i and slt ii), and enteroinvasiveness, and in bioassays for production of enterotoxins and cytotoxins, and for cell adheren ...	1992	1731056
cloning and expression of the variable regions of mouse myeloma protein mopc315 in e. coli: recovery of active fv fragments.	recombinant dna techniques were used to clone and express the fv portion of mopc315, a mouse myeloma protein with a high affinity for 2,4-dinitrophenyl (dnp). the fv fragment consists of a heterodimer of heavy and light chain variable domains (vh and vl). two separate bacterial plasmid constructs, containing either a variable region cdna for the light chain or a variable region synthetic gene for the heavy chain demonstrated high levels of expression (150-200 mg/l) under control of the bacteriop ...	1992	1731188
a fully active variant of dihydrofolate reductase with a circularly permuted sequence.	the amino acid sequence of mouse dihydrofolate reductase was permuted circularly at the level of the gene. by transposing the 3'-terminal half of the coding sequence to its 5' terminus, the naturally adjacent amino and carboxyl termini of the native protein were fused, and one of the flexible peptide loops at the protein surface was cleaved. the steady-state kinetic constants, the dissociation constants of folate analogues, and the degree of activation by both mercurials and salt as well as the ...	1992	1737018
development and characterization of a panel of monoclonal antibodies against the novel subtilisin-like proprotein processing enzyme furin.	monoclonal antibodies were raised against the recently discovered subtilisin-like proprotein processing enzyme furin. as immunogen, a bacterially expressed hybrid protein was used which consisted of glutathione s-transferase fused to almost the entire human furin protein. ten monoclonal antibodies were obtained and these could be divided into four categories on the basis of their reactivity towards a number of bacterially expressed hybrid proteins, each of which contained a different portion of ...	1992	1737642
synthesis of a functional anti-phytochrome single-chain fv protein in transgenic tobacco.	we have expressed a synthetic gene that encodes an antigen-binding single-chain fv protein (scfv) in transgenic tobacco plants. the scfv gene was created by polymerase chain reaction (pcr) amplification of the variable domain coding regions from a mouse monoclonal hybridoma cell line. the monoclonal cell line secretes an igg1 antibody that binds to the plant regulatory photoreceptor protein, phytochrome. the cloned scfv gene was expressed initially in escherichia coli and shown to produce a 28 k ...	1992	1368269
overproduction of biologically-active human nerve growth factor in escherichia coli.	a gene coding for human nerve growth factor (hngf) was constructed for expression under control of the trp promoter in e. coli. the plasmid ptrsngf contained a synthetic hngf gene fused, in frame, to the region encoding the beta-lactamase signal peptide. the plasmid ptrlngf contained the same coding sequence as hngf attached downstream from the n-terminal fragment of the trp l gene. e. coli cells harboring ptrsngf produced an amount of hngf constituting 4% of the total cellular protein, and remo ...	1992	1369095
expression of escherichia coli homoserine kinase in mouse 3t3 cells.	the escherichia coli gene for homoserine kinase (thrb) has been cloned into a simian-virus-40-based eukaryotic expression vector which also includes a neomycin-resistance gene. mouse 3t3 cells transfected with this plasmid were selected for resistance and screened for homoserine kinase activity. it has thus been possible to isolate clones which are capable of accumulating homoserine o-phosphate when supplied with homoserine. in broken-cell preparations the kinetic constants for the production of ...	1992	1371387
purification and characterization of soluble forms of human and rat stem cell factor recombinantly expressed by escherichia coli and by chinese hamster ovary cells.	stem cell factor (scf) is a novel, early-acting hematopoietic factor. it was isolated from the medium of a rat cell line in a soluble, processed form (zsebo et al., 1990, cell 63, 195). the cloned human and rat genes encode the soluble form plus additional c-terminal amino acids including a hydrophobic transmembrane domain (martin et al., 1990, cell 63, 203). we have recombinantly expressed forms of human and rat scf corresponding to the soluble, processed form in escherichia coli and in chinese ...	1992	1374224
molecular and immunological characterization of adp-ribosylarginine hydrolases.	mono-adp-ribosylation is a reversible modification of proteins with nad:arginine adp-ribosyltransferases and adp-ribosylarginine hydrolases catalyzing the forward and reverse reactions, respectively. hydrolase activities were present in a variety of animal species, with the highest specific activities found in rat and mouse brain, spleen, and testis. rat and mouse hydrolases were dithiothreitol- and mg(2+)-dependent, whereas the bovine and guinea pig enzymes were dithiothreitol-independent. a ra ...	1992	1375222
a molecular defect in human protoporphyria.	protoporphyria is generally an autosomal dominant disease that is characterized clinically by photosensitivity and hepatobiliary disease and that is characterized biochemically by elevated protoporphyrin levels. the enzymatic activity of ferrochelatase, which catalyzes the last step in the heme biosynthetic pathway, is deficient in all tissues of patients with protoporphyria. in this study, sequencing of ferrochelatase cdnas from a patient with protoporphyria revealed a single point mutation in ...	1992	1376018
monoclonal antibodies against bacterial glucosamine 6-phosphate synthase: production and use for structural studies.	fifteen mouse x rat hybridoma cell lines producing rat monoclonal antibodies (mabs) directed to escherichia coli glucosamine 6-p synthase (glms) were established and characterized. most of them (13/15) are igg2a while 2 were typed as igg1. their kaff ranged from 1.5 x 10(6) to 9.6 x 10(8) m-1 as determined by beatty et al. (1). the epitopes recognized by these mabs were assigned to one of the two catalytical domains of the enzyme (ct1 and ct2) as demonstrated both by elisa and western-blotting u ...	1992	1376719
directly acting geno- and cytotoxic agents from a wild mushroom dermocybe sanguinea.	the wild mushroom, dermocybe sanguinea, contains several anthraquinone pigments, of which emodin (1,3,8-trihydroxy-6-methylanthraquinone) is quantitatively the most important. in our preliminary tests, dermocybe sanguinea extracts were genotoxic without metabolic activation. the ethanol extract of dermocybe sanguinea was fractionated by flash chromatography, and the emodin contents of the fractions were determined by hplc. their genotoxicities were assayed using a bacterial repair assay and sist ...	1992	1381468
flow cytometric detection of drugs altering the dna methylation pattern.	we have developed a model system for assessing the demethylating potential of external agents. disruption in the dna methylation pattern was evaluated at the translational level of the escherichia coli beta-galactosidase coding gene (lacz). we have constructed a clonal cell line (a4/4 cells) derived from the adenovirus-transformed human embryonic kidney 293 strain. the a4/4 cells contain the e. coli lacz gene under the control of the mouse metallothionein 1 promoter which is down-regulated by a ...	1992	1382840
reactivity of monoclonal antibody e5 with endotoxin. i. binding to lipid a and rough lipopolysaccharides.	the murine igm monoclonal antibody (mab) e5 was produced by a hybridoma derived from spleen cells of a mouse immunized with the j5 rough mutant of escherichia coli o111:b4. in a multicenter randomized placebo-controlled clinical trial, e5 has been shown to reduce significantly the mortality and morbidity of patients with gram-negative sepsis. the characteristics of e5 binding to endotoxin were studied in vitro. we report here the results of binding to an extensive panel of rough lipopolysacchari ...	1992	1394865
analysis of human/mouse interleukin-6 hybrid proteins: both amino and carboxy termini of human interleukin-6 are required for in vitro receptor binding.	the multifunctional cytokine interleukin-6 (il-6) is a single polypeptide chain consisting of 184 amino acids in man and 187 amino acids in mouse. despite the relatively high degree of sequence similarity of these two molecules (about 57%), the biological activity in mouse and human il-6 shows species specificity. starting with this observation, we constructed interspecies hybrids with the goal of defining which segments of the human il-6 molecule are involved in human receptor binding. in this ...	1992	1396966
some properties of purified escherichia coli heat-stable enterotoxin ii.	we examined the biological properties of purified escherichia coli heat-stable enterotoxin ii (stii) using mouse intestinal loop assays and compared these properties with those of heat-stable enterotoxin i (sti) and cholera toxin (ct). the action of stii over time differed from those of sti and ct. stii did not alter cyclic gmp or cyclic amp levels in intestinal mucosal cells. our results supported the idea that the mechanism of action of stii in inducing fluid secretion is different from the me ...	1992	1398961
localization of infection using streptavidin and biotin: an alternative to nonspecific polyclonal immunoglobulin.	since favorable images of infection are obtained with radio-labeled nonspecific igg, streptavidin has been considered as an alternative protein in this investigation. the advantage of streptavidin is that once localized it may be targeted with radiolabeled biotin. studies were conducted in a mouse model with an escherichia coli infection in one thigh. indium-111-labeled streptavidin showed equivalent localization to the infection as that obtained with 111in-labeled polyclonal nonspecific igg, ho ...	1992	1403148
antibacterial effect of bovine milk antibody against escherichia coli in a mouse indigenous infection model.	a skim-milk fraction and a whey-protein concentrate (wpc) fraction were prepared from the cows that had been immunized with e. coli isolated from the mouse intestine. the antibacterial effect of these fractions against e. coli was examined. they contained antibody with a high affinity for e. coli strain 48, a representative strain in the mouse intestine, which is composed of a large amount of igg and smaller amounts of iga and igm. although these fractions showed no bactericidal or bacteriostati ...	1992	1406460
precursor structure, expression, and tissue distribution of human guanylin.	heat-stable enterotoxins (sta) are small, cysteine-rich peptides secreted by escherichia coli that are able to induce diarrhea through the stimulation of an intestine-specific receptor-guanylyl cyclase known as star. a 15-amino acid peptide, guanylin, was recently purified from rat jejunum and proposed to be a potential endogenous activator of this receptor. we describe here the cloning and characterization of human and mouse cdnas encoding precursor proteins of 115 and 116 amino acids, respecti ...	1992	1409606
bacterial single-chain antibody fragments, specific for carcinoembryonic antigen.	we have produced single-chain antibody (scfv) fragments in bacteria specific for carcinoembryonic antigen (cea). polymerase chain reaction (pcr) was used for the cloning and modification of the heavy and light variable regions (vh and vl) of the mouse monoclonal antibody (mab) cb-cea.1. a 14-amino acid linker was used in the synthesis of the scfv gene. the vh and vl regions were amplified from cdna by pcr using 5' end fr1 and 3' end constant region primers, and then sequenced. vh was then amplif ...	1992	1418981
unexpected position-dependent expression of h-2 and beta 2-microglobulin/lacz transgenes.	in order to study sequences involved in the developmentally regulated and tissue-specific expression of the class i major histocompatibility complex (mhc) genes, we have constructed several h-2/lacz transgenic lines in which the 5' regulatory sequences of the h-2kb gene are linked to the escherichia coli beta-galactosidase (lacz) gene. in five h-2/lacz lines, the pattern of lacz expression, detected histochemically varied greatly from line to line. none of the h-2/lacz transgenes were transcribe ...	1992	1418983
biochemical and immunological characterization of the dna binding protein (rbp-j kappa) to mouse j kappa recombination signal sequence.	we have investigated whether j kappa recombination signal sequence (rs) binding protein (rbp-j kappa) has any partial catalytic activities involved in the vdj recombination reaction, such as cleavage, ligation, and bending of dna. murine rbp-j kappa protein purified by j kappa-rs affinity chromatography did not show dna cleavage activities but contained a strong dna ligase activity. to obtain a large amount of purified rbp-j kappa protein, recombinant rbp-j kappa was synthesized in escherichia c ...	1992	1429518
alanine scanning mutagenesis identifies surface amino acids on domain ii of pseudomonas exotoxin required for cytotoxicity, proper folding, and secretion into periplasm.	pseudomonas exotoxin a (pe) is a single polypeptide chain that contains 613 amino acids and is arranged into three major structural domains. domain ia is responsible for cell recognition, domain ii for translocation of pe across the membrane, and domain iii for adp-ribosylation of elongation factor 2. recombinant pe can be produced in escherichia coli and is efficiently secreted into the periplasm when an ompa signal sequence is present. to investigate the role of the amino acids located on the ...	1992	1429683
[significance of phenotypical properties in extra-intestinal e. coli infections].	virulence factors (serogroup, haemolysis, haemagglutination, antigens k1, k5, colicinogenicity) and their association with diseases of 3334 escherichia coli strains isolated from different clinical specimens between 1979-1990 were analysed. strains, that were isolated from cerebrospinal fluid of newborns under one month were characterized by certain serogroups (o7, o18, o45, o78, o83), possession of antigen k1 and production of colicin. on the basis of their ld50 they belonged to the pathogenic ...	1992	1437114
rfp is a dna binding protein associated with the nuclear matrix.	we reported that the rfp gene encodes a protein with putative zinc finger domains and was involved in the activation of the ret proto-oncogene. to further characterize the rfp protein, we developed a polyclonal antibody against the product synthesized from a fragment of the rfp cdna expressed in escherichia coli. western blot analysis showed that rfp was identified as a 58 kda protein in cell lysates from four human and rodent cell lines and from mouse testis. in addition, a unique 68 kda protei ...	1992	1437549
genetic control of resistance to enterotoxigenic escherichia coli in infant mice.	dba/2 and cba infant mice orally challenged with bovine enterotoxigenic escherichia coli (etec) strain b80 presented resistance and susceptibility respectively, as measured by mortality rates 6 days after inoculation. serum antibodies agglutinating etec strain b80 had very low titers in both mouse strains. mendelian analysis of resistance on f1 and on segregating back-crosses showed that resistance is genetic and dominant. dominance may be explained either by a mixed control with an overdominant ...	1992	1453928
site-directed mutation makes rabbit calcyclin dimer.	unlike human, rat and mouse calcyclin, purified rabbit calcyclin did not form a dimer on tricine sds-page under non-reduced conditions. based on the internal peptide sequence of rabbit calcylin, we isolated and sequenced a cdna clone encoding calcyclin. the sequence of this clone (pcalc) is 629 bp long and codes 90 amino acid residues of a protein with a molecular mass of 10,153 da. by northern blot analysis, a major band of 0.9 kbp and a minor band of 2.6 kbp were detected in the lung. the reco ...	1992	1459239
high affinity ligand binding is not essential for granulocyte-macrophage colony-stimulating factor receptor activation.	the high affinity receptor of the cytokine granulocyte-macrophage colony-stimulating factor (gm-csf) is a heterodimer composed of two members of the cytokine receptor superfamily. gm-csf binds to the alpha-subunit (gm-r alpha) with low affinity and to the receptor alpha beta complex (gm-r alpha beta) with high affinity. the gm-csf.gm-r alpha beta complex is responsible for biological activity. interactions of the n-terminal helix of mouse gm-csf with mgm-r alpha beta were examined by introducing ...	1992	1460041
[virulence factors in strains of escherichia coli isolated in urinary tract infections].	in 21 strains of e. coli isolated from patients with cystitis and asymptomatic bacteriuria the authors assessed the group, mannososensitive and mannoresistant agglutination of human, bovine, chick, guinea pig, sheep and pig erythrocytes, the production of haemolysis, colicins, aerobactin, the capacity of strains to induce oedema on mouse paws, the lethal effect in mice, the resistance to tetracycline, streptomycin, chloramphenicol, kanamycin, ampicillin and sulfamethoxidine, the transmission of ...	1992	1464082
targeting sequences of the two major peroxisomal proteins in the methylotrophic yeast hansenula polymorpha.	dihydroxyacetone synthase (das) and methanol oxidase (mox) are the major enzyme constituents of the peroxisomal matrix in the methylotrophic yeast hansenula polymorpha when grown on methanol as a sole carbon source. in order to characterize their topogenic signals the localization of truncated polypeptides and hybrid proteins was analysed in transformed yeast cells by subcellular fractionation and electron microscopy. the c-terminal part of das, when fused to the bacterial beta-lactamase or mous ...	1992	1465101
[adherence of e. coli to bladder epithelia in compromised mice and plasma endotoxin level].	the role of bacterial adherence in association with complicated urinary tract infections (uti) and the correlation between bacterial adherence and plasma endotoxin (et) levels were experimentally investigated by using mouse uti models. mice with foreign bodies induced in the bladder or voiding dysfunction were more susceptible to uti than untreated mice. but diabetic or granulocytopenic mice were little susceptible to uti in comparison with other two models. adherence activities of 6 strains of ...	1992	1474705
molecular analysis of the aroa gene of pasteurella multocida and vaccine potential of a constructed aroa mutant.	the aroa gene from pasteurella multocida was cloned by complementation of the escherichia coli aroa mutant ab2829 with a dna library constructed in puc18. the nucleotide sequence of the p. multocida aroa gene indicated an open reading frame encoding a protein of 441 amino acids, which showed a high degree of homology with the amino acid sequences of various other bacterial aroa proteins. the cloned p. multocida aroa gene was inactivated by insertion of a kanamycin-resistance gene and reintroduce ...	1992	1474900
high affinity dna-microtubule interactions: evidence for a conserved dna-map interaction involving unusual high cscl density repetitious dna families.	we have examined high affinity interactions of chick brain microtubule proteins with 35s labelled tracer dnas from chick, mouse and d. melanogaster under equilibrium conditions by the nitrocellulose filter binding technique. ternary reaction mixtures of the above two components and a third component, an excess of unlabelled competitor dna from either e. coli., mouse, d. melanogaster or chick, were used to measure small fractions of dna in each case (1-4%) bound to microtubule protein under high ...	1992	1488054
effect of serum amyloid p component level on transthyretin-derived amyloid deposition in a transgenic mouse model of familial amyloidotic polyneuropathy.	to elucidate the pathogenesis of amyloid deposition associated with familial amyloidotic polyneuropathy (fap), we developed several transgenic mouse lines carrying the human mutant transthyretin (ttr) gene. we found that human ttr and mouse serum amyloid p component (sap) are deposited as amyloid in tissues of these mouse lines. because sap is a major acute-phase reactant in mice, we asked whether repeated injections of escherichia coli lipopolysaccharide (lps) would enhance the amyloid depositi ...	1992	1497094
effect of ambient temperature and e. coli endotoxin upon the plasma iron level in wild house mice in winter season.	the effects of ambient temperatures of 10 degrees c and 30 degrees c and of e. coli endotoxin on brain temperature and plasma iron level were investigated in unrestrained wild house mice, mus musculus. in control animals (i.p. saline-injected) exposed to cold environment the brain temperature decreased and plasma iron levels were lower than those observed under thermoneutral conditions (30 degrees c). animals injected i.p. with endotoxin (0.5 micrograms.kg-1) and placed at 30 degrees c showed a ...	1992	1506489
crystallization studies of the catalytic subunit of camp-dependent protein kinase: crystals of murine recombinant catalytic subunit and a mutant, cys 343----ser, diffract to 2.7 a resolution.	the recombinant mouse catalytic subunit of camp-dependent protein kinase, expressed and purified from e. coli, has been successfully cocrystallized as a binary complex with an inhibitor peptide and as a ternary complex with an inhibitor peptide and mgatp. in contrast to the catalytic subunit obtained from porcine heart, the recombinant catalytic subunit lacks a myristoyl group at the amino terminus and differs in sequence at nine positions out of 350 amino acids. the catalytic activities of the ...	1992	1515113
response elements of the androgen-regulated c3 gene.	intron and 5'-flanking regions of the androgen-regulated c3 subunit gene contain potential cis-acting transcription control sequences including several 15-base pair (bp) partial palindromes resembling response elements for glucocorticoid (gre) and progesterone (pre) receptors. specific dna binding of the androgen receptor (ar) and androgen-dependent activation of transcription indicate that some of these gre/pre-like sequences are capable of functioning as androgen response elements (are). a 0.3 ...	1992	1537831
a streptavidin-metallothionein chimera that allows specific labeling of biological materials with many different heavy metal ions.	we have designed a streptavidin-metallothionein chimeric protein in which the streptavidin moiety provides a means of binding the metallothionein moiety tightly to specific biological targets. a gene fusion of streptavidin with mouse metallothionein i was efficiently expressed in escherichia coli, and the expressed chimeric protein was purified to homogeneity by a simple procedure. the purified chimera, consisting of four identical subunits, bound one biotin and approximately seven cd2+ ions per ...	1992	1542645
high-fat diets and the immune response of c57bl mice.	as a basis for studies of the influence of lipids on the immune response and health, adult c57bl mice were fed for 10 weeks or longer on one of the following diets: high (200 g/kg) polyunsaturated fatty acid, high (200 g/kg) saturated fatty acid and low (50 g/kg) polyunsaturated fatty acid purified diets and a standard commercial diet. the three test-fat diets were compounded to have approximately the same energy content and the mice of each group maintained similar body-weights. high-fat diets ...	1992	1547199
detailed histopathological examination contributes to the assessment of escherichia coli urovirulence.	to determine the usefulness of detailed histopathological evaluation in the assessment of urovirulence of different escherichia coli strains in a mouse model of ascending, unobstructed urinary tract infection, and to evaluate the relationship between susceptibility to urinary tract infection and renal levels of p fimbrial receptor glycolipids in different mouse strains, female swiss webster and balb/c mice were inoculated transurethrally with one of four different well-characterized wild type e. ...	1992	1552614
high-level temperature-induced synthesis of an antibody vh-domain in escherichia coli using the pelb secretion signal.	we have constructed a temperature-inducible escherichia coli expression vector (ppow) for enhanced secretion of antibody (ab) domains and other foreign proteins. the vector contains the lambda prpl promoters in tandem, and the ci857 gene encoding the temperature-sensitive repressor which provide tight control over protein production. the pelb secretion signal directs the synthesized foreign protein through the cytoplasmic membrane. a mouse ab fragment (the variable heavy (vh) domain of nc41) was ...	1992	1563636
immune response of cattle to haemophilus somnus lipid a-protein conjugate vaccine and efficacy in a mouse abortion model.	immunogenicity of the lipid a component of haemophilus somnus lipooligosaccharide in cattle and mice was examined after purification, detoxification, and covalent conjugation to a protein carrier. after 2 inoculations, a substantial antibody response was induced in most cattle to lipid a and the protein carrier. to determine whether antibodies to lipid a would be protective, 5 x 10(7) colony-forming units of h somnus strain 649 were administered iv to endotoxin-responsive (c3h/hen) mice. in one ...	1992	1575380
engineered disulfide bond greatly increases specific activity of recombinant murine interferon-beta.	unlike other species of interferon-beta (ifn-beta) mouse (mu) ifn-beta has no naturally occurring intramolecular disulfide bond. when expressed in escherichia coli, muifn-beta appears to exhibit instability and low activity. to increase its activity, we engineered a pair of cysteines into recombinant muifn-beta to test whether this change would improve its antiviral activity. in the absence of detailed structural data, the optimal placement of cysteines was determined by sequence comparison with ...	1992	1578187
lps genotype in the c57 black mouse background and its influence on the interleukin-6 response to e. coli urinary tract infection.	the present study examined the influence of the mouse lps genotype on the interleukin-6 (il-6) and polymorphonuclear leucocyte (pmn) responses to mucosal escherichia coli infection. lipopolysaccharide (lps) responder c57bl/6j (lpsn, lpsn) and lps non-responder c57bl/10sccr (lpsd, lpsd) mice were inoculated intravesically with escherichia coli hu734. the secretion of il-6, the recruitment of pmns into urine, and the bacterial clearance from the kidneys and bladders were compared between the two m ...	1992	1579857
plasmodium falciparum: the repetitive msa-1 surface protein of the ro-71 isolate is recognized by mouse antibody against the nonrepetitive repeat block of ro-33.	we have expressed in escherichia coli the nonrepetitive repeat zone of the msa-1 surface protein of the ro-33 isolate of plasmodium falciparum. the recombinant protein was used to immunize mice and the resulting ro-33 monospecific serum was used to screen our p. falciparum strain collection in order to recover additional alleles lacking tripeptide repeats in block 2 of msa-1. only 1 (ro-71) out of 30 isolates tested reacted strongly with the serum by indirect immunofluorescence assay. surprising ...	1992	1592091
gas2, a growth arrest-specific protein, is a component of the microfilament network system.	in this report we analyze the protein product of a growth arrest-specific gene, gas2, by means of an affinity-purified antibody raised against the protein produced in bacteria. the regulation of gas2 biosynthesis reflects the pattern of mrna expression (schneider, c., r. king, and l. philipson. 1988. cell. 54:787-793): its relative level is tightly associated with growth arrest. gas2 seems to be regulated also at the posttranslational level via a phosphorylation mechanism. gas2 is well conserved ...	1992	1607387
enhanced growth restriction of legionella pneumophila in endotoxin-treated macrophages.	macrophages from a/j mice are permissive for growth of legionella pneumophila, an intracellular opportunistic pathogen that grows preferentially in macrophages. macrophages from other mouse strains are highly resistant to growth of legionella. in the present study, it was found that macrophages from a/j mice are readily activated by pretreatment with lipopolysaccharide (lps), so that the cells do not permit legionella to replicate in vitro, as occurs when untreated macrophages from a/j mice are ...	1992	1615009
rat tumor necrosis factor-alpha. transcription in rat kupffer cells and in vitro posttranslational processing based on a pcr-derived cdna.	a dna fragment with a reading frame of 708 basepairs coding for tnf-alpha from rat liver was cloned by the polymerase chain reaction. using this species-specific cdna a biotin-labelled antisense (-)rna was transcribed. this probe was used for northern blot analysis of tnf-alpha gene activation. exposure of rat kupffer cells to lps led to a time-dependent change of tnf-alpha-mrna expression with a maximum between one and two hours after stimulation. in vitro translation was carried out with sense ...	1992	1627266
shigella-type pathomechanism in the "mouse model".	a strain of escherichia coli k-12 carrying the 140-megadalton virulence plasmid of the enteroinvasive e. coli--j53(pspl)--showed high virulence in the "mouse model", in chick embryos, but not in the serény test. it expresses the outer membrane proteins thermoregulatedly, encoded also by the virulence plasmid. in orally infected streptomycin-pretreated mice this strain infects only the large bowel, shows adherence to the epithelial surface, but in its first step preferentially to the mucus excret ...	1992	1632198
mapping of a region of dengue virus type-2 glycoprotein required for binding by a neutralizing monoclonal antibody.	envelope glycoprotein e of flaviviruses is exposed at the surface of the virion, and is responsible for eliciting a neutralizing antibody (ab) response, as well as protective immunity in the host. in this report, we describe a method for the fine mapping of a linear sequence of the e protein of dengue virus type-2 (den-2), recognized by a type-specific and neutralizing monoclonal ab (mab), 3h5. first, an escherichia coli expression vector containing a heat-inducible lambda pl promoter was used t ...	1992	1634111
lipopolysaccharide priming of alveolar macrophages for enhanced synthesis of prostanoids involves induction of a novel prostaglandin h synthase.	we report here that lipopolysaccharide (lps) priming of rabbit alveolar macrophages leads to amplified synthesis of prostanoids, at least in part, by induction of a novel prostaglandin h synthase (pgh synthase). rabbit alveolar macrophages were cultured with or without added lps derived from escherichia coli 0111:b4 for 4 h and then stimulated with opsonized zymosan (opz). lps priming of alveolar macrophages resulted in enhanced release of thromboxane (tx) upon stimulation with opz, when compare ...	1992	1634505
t and b cell epitope mapping of sm23, an integral membrane protein of schistosoma mansoni.	sm23 is an integral membrane protein of the blood-vessel dwelling parasitic worm schistosoma mansoni. this protein has been detected with antibodies in all stages of the parasite found in the human host, notably the lung stage, and therefore is of interest as a vaccine candidate. in addition sm23 has been shown to be a member of a proposed new superfamily of membrane proteins whose structures do not conform to the previously known classifications. to date there are 13 members including me491 (cd ...	1992	1281197
cloning of the vaccinia virus ribonucleotide reductase small subunit gene. characterization of the gene product expressed in escherichia coli.	during its infectious cycle, vaccinia virus expresses a virus-encoded ribonucleotide reductase which is distinct from the host cellular enzyme (slabaugh, m.b., and mathews, c.k. (1984) j. virol. 52, 501-506; slabaugh, m.b., johnson, t.l., and mathews, c.k. (1984) j. virol. 52, 507-514). we have cloned the gene for the small subunit of vaccinia virus ribonucleotide reductase (designated vvr2) into escherichia coli and expressed the protein using a t7 rna polymerase plasmid expression system. afte ...	1992	1309792
regulation of the escherichia coli lac operon expressed in human cells.	we have investigated the use of various epstein-barr virus (ebv)-based vectors bearing the two components of the escherichia coli lac operator-repressor (laco, laci) complex. our aim was to develop a model system of gene expression by looking at the transcription of the bacterial beta-galactosidase coding gene (lacz) in 293 human embryonic kidney cells. several vectors have been built carrying different promoters upstream of the laci and lacz genes and in which natural or synthetic operator sequ ...	1992	1311956
termination complex in escherichia coli inhibits sv40 dna replication in vitro by impeding the action of t antigen helicase.	dna replication terminus (ter)-binding protein (tbp) in escherichia coli binds specifically to the terminus (ter) site, and the resulting complex severely blocks dna replication in an unique orientation by inhibiting the action of helicases. to generalize the intrinsic nature of the orientated ter-tbp complex against various helicases, we tested the potential of the complex to inhibit the action of three helicases, dna helicase i, simian virus 40 (sv40) large tumor (t) antigen, and helicase b, d ...	1992	1312088
a mouse cdc25 homolog is differentially and developmentally expressed.	the timing and activation of the p34cdc2 kinase in mammals is associated with dephosphorylation of phosphotyrosine and phosphothreonine residues on the p34cdc2 kinase. for fission yeast, the timing of mitosis is regulated by cyclic accumulation of cdc25, which promotes dephosphorylation of p34cdc2 and concomitant protein kinase activation. we report the identification and characterization of a structural and functional mouse homolog, cdc25m2, of the cdc25 phosphatase. cdc25m2 shows high sequence ...	1992	1313771
in vitro isoprenylation and membrane association of mouse rod photoreceptor cgmp phosphodiesterase alpha and beta subunits expressed in bacteria.	we investigated the specificity of caax box-related isoprenylation of rod photoreceptor cgmp phosphodiesterase (pde) subunits expressed in bacteria and the consequences of this modification on rod disk membrane association. full-length cdna sequences of the alpha and beta subunits of mouse pde, inserted into bacterial pet expression vectors, were overexpressed as fusion proteins containing 28 (bmp-alpha) and 26 (bmp-beta) additional amino acid residues at their n termini. both fusion proteins we ...	1992	1314827
expression and purification of mouse timp-1 from e. coli.	tissue inhibitors of metalloproteinases (timps) constitute a family of secreted glycoproteins involved in regulating extracellular matrix degradation in both normal and malignant tissues. we have expressed a cdna clone of mouse timp-1 as a 22-kda protein with 12 cysteine residues in e. coli and purified protein that shows inhibitory activity against collagenase following renaturation by chemical means. the low specific activity and circular dichroism measurements suggest, however, that the renat ...	1992	1322839
molecular cloning of human macrophage capping protein cdna. a unique member of the gelsolin/villin family expressed primarily in macrophages.	macrophage capping protein (mcp) is a ca(2+)-sensitive protein which reversibly blocks the barbed ends of actin filaments but does not sever preformed actin filaments. the human cdna for mcp has been cloned and sequenced. the derived amino acid sequence predicts a polypeptide of 38.4 kda. human mcp expressed in escherichia coli using a pet12a vector was functionally identical to the native protein purified from rabbit alveolar macrophages with respect to ca2+ sensitivity and ability to block mon ...	1992	1322908
establishment of a mouse model of cystitis and roles of type 1 fimbriated escherichia coli in its pathogenesis.	the role of type 1 fimbriae in promoting bladder colonization and the course of escherichia coli cystitis were examined with type 1 fimbriated strains of clinically isolated e. coli. in the experiments of mice in vivo, intact bladder epithelium showed natural resistance to the adherence of type 1 fimbriated and non-fimbriated e. coli. however, the exfoliation of bladder superficial cells by trypsinization before the bacterial inoculation promoted the adhesion and colonization of type 1 fimbriate ...	1992	1351243
the beta 1 integrin distal promoter is developmentally regulated in transgenic mice.	transgenic mice harbouring 5' flanking sequences of the human beta 1 integrin gene linked to the escherichia coli lacz gene have been generated to examine spatial and temporal distribution of the promoter activity during development. our previous data showed that this regulatory region is composed by two promoters, called distal and proximal, located closely on the human genome. to determine the role of each promoter region during development we generated transgenic mice using these two sequence ...	1993	7521754
embryonic expression of human keratin 18 and k18-beta-galactosidase fusion genes in transgenic mice.	during embryogenesis, endob, the mouse form of human keratin 18 (k18), is expressed in a complex spatial and temporal pattern in various embryonic epithelia. we have compared the expression of transgenic human k18 to the endogenous mouse homolog and to the coexpressed, complementary keratin 8 homolog, endoa, during postimplantation mouse embryogenesis and fetal development in order to determine the developmental expression pattern of the human gene in a mouse environment. the tissue distribution ...	1993	7504637
epitope mapping and accessibility of immunodominant regions of yeast plasma membrane h(+)-atpase.	immunodominant regions of yeast plasma membrane h(+)-atpase have been mapped by two different approaches. a rabbit polyclonal antibody was used to screen a library of random fragments of the atpase gene in a bacterial expression plasmid. in addition, the epitopes recognized by a panel of mouse monoclonal antibodies against the atpase were mapped by reactions with defined fragments of the enzyme expressed in escherichia coli. both methodologies indicated that two regions within the amino-terminal ...	1993	7681777
assembly of gabaa receptor subunits: analysis of transient single-cell expression utilizing a fluorescent substrate/marker gene technique.	gabaa receptor channels (gabars) composed of varying combinations of alpha 1, beta 1, and gamma 2s subunits were transiently expressed in mammalian cell lines. the whole-cell patch-clamp recording technique was used to determine which combinations of gabar subunits produced functional receptor channels and whether assembly of gabar subunits into receptor channels followed a random or preferred sequence. to identify rapidly cells expressing gabars, mammalian cell lines were cotransfected with com ...	1993	7681869
cdna for r-cognin: homology with a multifunctional protein.	retina cognin (r-cognin) is a developmentally regulated 50-kda protein that was isolated from chicken embryo retina cell membranes. it mediates the adhesion and reaggregation in vitro of retina cells from chicken and mouse embryos, but not of cells from other tissues, and may be involved in neuronal differentiation. we report here the cloning of a cdna for r-cognin. a chicken embryo retina cdna library was constructed in lambda gt11 vector and was screened with polyclonal r-cognin antiserum, yie ...	1993	7681992
translational repression of endogenous thymidine kinase mrna in differentiating and arresting mouse cells.	we observed that decline of thymidine kinase (tk) enzyme activity was severalfold faster than the decay of full length tk mrna during growth arrest of 3t6 mouse fibroblasts or during differentiation of myoblasts (c2cl12) or f9 embryonal carcinoma cells. in order to study the molecular mechanism of this disparate behavior, a polyclonal antiserum against mouse tk was raised in rabbit. high level expression of mouse tk polypeptide in escherichia coli was achieved with a t7 rna polymerase-directed e ...	1993	7684382
differences in cytokine response and induction of nitric oxide synthase in endotoxin-resistant and endotoxin-sensitive mice after intravenous gram-negative infection.	previous reports have suggested that the endotoxin-resistant c3h/hej strain of mouse is more susceptible to infection than is the endotoxin-sensitive parent strain, c3h/hen, although they have never been compared in an i.v. model of sepsis. we therefore have used these mouse strains in an i.v. model of gram-negative sepsis to compare their sensitivities to infection, their cytokine responses, and the levels of induction of the enzyme nitric oxide synthase assayed in their livers. by using i.v. i ...	1993	7684416
biological activity of recombinant human myelin basic protein.	using an inducible expression vector in escherichia coli, we have expressed, purified, and biologically characterized recombinant human myelin basic protein (r-mbp). the recombinant protein binds in cation-exchange chromatography with similar affinity to purified, human mbp, and elutes as a single, 18.5-kda species as judged by sds-page. the recombinant protein exhibits similar conformation to native mbp, as demonstrated by elisa reactivity with a panel of six monoclonal antibodies directed agai ...	1993	7685037
rnase mrp and rnase p share a common substrate.	rnase mrp is a site-specific ribonucleoprotein endoribonuclease that processes rna from the mammalian mitochondrial displacement loop containing region. rnase p is a site-specific ribonucleoprotein endoribonuclease that processes pre-trnas to generate their mature 5'-ends. a similar structure for the rnase p and rnase mrp rnas and a common cleavage mechanism for rnase mrp and rnase p enzymes have been proposed. experiments with protein synthesis antibiotics have shown that both rnase mrp and rna ...	1993	7688115
ntp technical report on the toxicity studies of a chemical mixture of 25 groundwater contaminants administered in drinking water to f344/n rats and b6c3f(1) mice.	toxicity studies were performed with a chemically defined mixture of 25 groundwater contaminants, using dose levels considered to have environmental relevance. the mixture contained 19 organic compounds and six metals (shown below); the selection of these compounds was based primarily on the frequency of their occurrence in united states environmental protection agency surveys of groundwater contamination in the vicinity of hazardous waste disposal sites. this report focuses primarily on 26-week ...	1993	12209189
[study on infectious mechanism of type 1 fimbriated escherichia coli in experimental cystitis of mice].	the course of experimental cystitis in mice with escherichia coli was studied by electron microscopy. the inoculated e. coli did not adhere to the intact bladder epithelium. however, when the mouse bladder was treated with trypsin, superficial cells were exfoliated, and the inoculated type 1 fimbriated e. coli adhered to the exposed intermediate cells and caused cystitis. the nonfimbriated e. coli showing no adherence to the superficial cells or intermediate cells and failed to cause cystitis. t ...	1993	7909502
absence of persistence and transfer of genetic material by recombinant escherichia coli in conventional, antibiotic-treated mice.	strain bst-1 is a derivative of escherichia coli k-12 that carries a plasmid designated pura-4 and is the expression system used by the upjohn company in the production of recombinant bovine somatotropin (rbst). this plasmid also encodes an ampicillin resistance gene. the plasmidless carrier strain, bst-1c, contains a gene for tetracycline resistance which is provided by the chromosomal insertion of the transposon tn10. therefore, bst-1 is resistant to ampicillin and tetracycline, while bst-1c i ...	1993	7763898
safety assessment of the neomycin phosphotransferase ii (nptii) protein.	two approaches were used to assess the safety of the nptii protein for human consumption using purified e. coli produced nptii protein that was shown to be chemically and functionally equivalent to the nptii protein produced in genetically engineered cotton seed, potato tubers and tomato fruit. the nptii protein was shown, as expected, to degrade rapidly under simulated mammalian digestive conditions. an acute mouse gavage study confirmed that the nptii protein caused no deleterious effects when ...	1993	7764244
isolation of the chicken nf-kappa b p65 subunit-encoding cdna and characterization of its products.	nf-kappa b is a heterodimeric transcription factor consisting of subunits of 50 kda (p50) and 65 kda (p65). cdna clones encoding the chicken nf-kappa b p65 subunit were isolated. sequence analysis showed that chicken p65 is approximately 55% identical to the mouse and human p65 proteins, and contains the rel homology domain (rhd) in its n-terminal 286 amino acids (aa) and the putative transactivation domain in its c-terminal region. the rhd is particularly highly conserved between the chicken an ...	1993	7916720
utilization of the mouse large intestine to select an escherichia coli f-18 dna sequence that enhances colonizing ability and stimulates synthesis of type 1 fimbriae.	escherichia coli f-18, a normal human fecal isolate, is an excellent colonizer of the streptomycin-treated mouse large intestine. e. coli f-18 col-, a derivative of e. coli f-18 which no longer makes the e. coli f-18 colicin, colonizes the large intestine as well as e. coli f-18 when fed to mice alone but is eliminated when fed together with e. coli f-18. random sequences of e. coli f-18 dna were cloned into prlb2, a par-b-stabilized derivative of phc79. the entire gene library was transformed i ...	1993	8095923
ferrochelatase structural mutant (fechm1pas) in the house mouse.	the molecular basis of an inherited defect of ferrochelatase in mouse (fechm1pas/fechm1pas, described by tutois et al., 1991, j. clin. invest. 88:1730-1736) was investigated. cdna clones encoding ferrochelatase, isolated by amplification of the mrna from the liver of a mutant mouse using the polymerase chain reaction, were sequenced by the dideoxynucleotide chain-termination method. all the clones carried a t to a transversion at nucleotide 293, leading to a methionine to lysine substitution at ...	1993	8325637
interleukin-1 receptor antibody (il-1rab) protection and treatment against lethal endotoxemia in mice.	interleukin-1 (il-1) is a mediator of endotoxin shock and il-1 receptor blockade has been shown to have therapeutic efficacy against endotoxic shock and sepsis in laboratory models. the current studies were designed to characterize the efficacy of a murine monoclonal il-1 receptor antibody (il-1rab) against endotoxin (lps) lethality and to investigate whether combined anticytokine therapy using the il-1rab and a highly specific polyclonal rabbit anti-mouse tnf antibody (tnf ab) could provide add ...	1993	8331925
comparison of the relative toxicities of shiga-like toxins type i and type ii for mice.	in earlier studies using a streptomycin-treated mouse model of infection caused by enterohemorrhagic escherichia coli (ehec), animals fed shiga-like toxin type ii (slt-ii)-producing strains developed acute renal cortical necrosis and died, while mice fed shiga-like toxin type i (slt-i)-producing clones did not die (e. a. wadolkowski, l. m. sung, j. a. burris, j. e. samuel, and a. d. o'brien, infect. immun. 58:3959-3965, 1990). to examine the bases for the differences we noted between the two tox ...	1993	8335369
the post-antibiotic effect of antimicrobial combinations in a neutropenic murine thigh infection model.	the post-antibiotic effect (pae) may allow for more widely spaced dosing of antibiotics than is currently employed without loss of efficacy. antimicrobial combinations are widely used in clinical medicine. however, dosing schedules are usually based on pharmacological profiles of the drugs used alone. previously we have demonstrated significant prolongation of the pae induced by antimicrobial combinations in vitro as compared to paes induced by the agents alone. we examined this issue further in ...	1993	8335520
the 28-kda n-terminal domain of mouse stromelysin-3 has the general properties of a weak metalloproteinase.	the putative matrix metalloproteinase mouse stromelysin-3 was expressed from escherichia coli and from a mouse myeloma cell line. in the former case a single major protein of 58-kda was detectable by immunoblotting, but no proteolytic activity could be elicited by zymography or trypsin or organomercurial treatment as would be expected for a typical matrix metalloproteinase. in the latter case immunodetectable proteins of 55-58 and 27-28-kda were produced. the effect of trypsin or organomercurial ...	1993	8340372
o6-methylguanine-dna methyltransferase protects against nitrosamine-induced hepatocarcinogenesis.	we previously generated transgenic c3h/hen mice by introducing the escherichia coli o6-methylguanine-dna methyltransferase (mgmt, dna-o6-methylguanine:protein-l-cysteine s-methyltransferase, ec2.1.1.63) gene, ada, attached to the chinese hamster metallothionein i gene promoter. one transgenic mouse line expressing both ada-specific mrna and ada protein could be propagated over many generations in a homozygous state with respect to the integrated dna. liver extracts from transgenic homozygous mic ...	1993	8341657
identification of the product of the murine st2 gene.	the murine st2 gene is expressed in growth-stimulated balb/c-3t3 cells. this gene encodes a protein that is similar to the extracellular portions of the interleukin-1 receptors (types 1 and 2). in this study, we prepared a polyclonal antibody against the recombinant st2 protein produced in escherichia coli. this antibody detected recombinant st2 protein in the culture fluid of cos7 cells transfected with a mammalian expression vector (pef-bos) carrying st2 cdna. using this antibody, we could det ...	1993	8347678
identification and characterization of heat-stable enterotoxin ii-producing escherichia coli from patients with diarrhea.	stock strains of escherichia coli isolated from patients with traveller's diarrhea were examined for production of heat-stable enterotoxin ii (stii). of 400 strains examined, 3 were found to produce stii. the nucleotide sequence of the stii gene of these human strains was shown to be identical to that of porcine strains. cultured cells of these strains induced fluid accumulation in ligated mouse intestinal loops and the activity was neutralized by anti-stii antiserum. these results suggest that ...	1993	8355624