Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| isolation and molecular analysis of the orotidine-5'-phosphate decarboxylase gene (pyrg) of phycomyces blakesleeanus. | the pyrg gene of phycomyces was isolated from a phycomyces genomic library, constructed in the cosmid phs255, by hybridization with a 170 bp fragment of the pyrg gene of aspergillus niger. this fragment includes a consensus sequence found in almost all species in which the orotidine-5'-phosphate decarboxylase (ompdecase) gene has been sequenced. the complete nucleotide sequence of the cloned pyrg gene from phycomyces was determined and the transcription start sites mapped. in the predicted amino ... | 1990 | 2277645 |
| preparation and characterization of monoclonal antibodies to human hexokinase type i. | 1. three different immunization protocols and several screening procedures were used to prepare seven mouse monoclonal antibodies to human placenta hexokinase type i. none of these monoclonals were able to recognize the native enzyme but all detected hexokinase when adsorbed onto polystyrene plates or on immunoblots after sds/polyacrylamide-gel electrophoresis. 2. all seven monoclonals recognize the two different subtypes of human hexokinase i equally well. limited tryptic digestion of hexokinas ... | 1990 | 2280763 |
| human xq24-xq28: approaches to mapping with yeast artificial chromosomes. | one hundred twenty-seven yeast strains with artificial chromosomes containing xq24-xqter human dna were obtained starting from a human/hamster somatic cell hybrid. the clones were characterized with respect to their insert size, stability, and representation of a set of xq24-xqter dna probes. the inserts of the clones add up to 19.3 megabase (mb) content, or about 0.4 genomic equivalents of that portion of the x chromosome, with a range of 40-650 kb in individual yacs. eleven clones contained mo ... | 1990 | 2294758 |
| isolation and sequencing of nop1. a yeast gene encoding a nucleolar protein homologous to a human autoimmune antigen. | we have identified the gene for the yeast nucleolar protein p38 and deduced the primary structure of p38 from its sequence. we propose the name nop1 (nucleolar protein 1) for this gene. nop1 encodes a 327 amino acid protein of 34,470 daltons and is flanked by potential promoter and polyadenylation sequences. blot analyses indicate that the mrna transcribed from nop1 is approximately 1.3 kilobases in size and that there is one nop1 gene per haploid genome. the amino-terminal sequence of p38 is ho ... | 1990 | 2298745 |
| reactivation of neutron killed mammalian cells by gamma irradiation: the observations, possible mechanism and implication. | we have observed that combinations of neutron plus gamma ray exposure can significantly increase the colony forming ability of monkey and human cell cultures over the neutron dose alone. the "reactivation" of neutron killed mammalian cells by gamma rays is analogous to observations made in lower eukaryotic organisms and fits the pattern termed "t repair" previously postulated for yeast and protozoans. | 1990 | 2300886 |
| deficient lysosomal carboxypeptidase activity in galactosialidosis. | in the lysosome, the glycosidases neuraminidase (ec 3.2.1.18) and beta-galactosidase (ec 3.2.1.23) are associated to a 52 kda "protective protein" to form a large multi-enzymatic complex. deficient synthesis or inactivation of this protective protein causes galactosialidosis, a lysosomal storage disorder in man in which both neuraminidase and beta-galactosidase activities are deficient. since the protective protein possesses extensive sequence homology with carboxypeptidase y (carb y) and the ke ... | 1990 | 2328002 |
| nuclear proteins from hela cells are immunoreactive with anti-yeast yp20 ras related antibodies. | immunoblot analysis using antibody specific to yeast ras-related protein yp20 revealed a 21kda protein associated with hela cell chromatin which immunologically cross-reacted with this ras-related protein. the 21kda protein can be rapidly released from the hela nuclei by micrococcal nuclease digestion. it seems to be associated with long mononucleosomes as determined by separation of chromatin subunits on sucrose gradients. we suggest that the 21kda hela protein may be the human homologue of yea ... | 1990 | 2334423 |
| transcriptional activation domain of the muscle-specific gene-regulatory protein myf5. | the human muscle determination factor myf5, like myod and other members of the family of skeletal muscle-specific regulatory proteins, contains a highly conserved putative helix-loop-helix domain. in myod this motif is required for the initiation of myogenesis in c3h mouse 10t1/2 fibroblasts and other non-muscle cells as well as for transcriptional activation of muscle genes. high affinity dna binding of myod to regulatory dna elements in muscle genes requires the formation of heterodimers with ... | 1990 | 2385294 |
| [circulating immune complexes and myasthenia gravis (analysis of 78 cases)]. | for the sake of studying the circulating immune complexes (cic) of 78 patients with myasthenia gravis (mg), the agglutination assay of c3b sensitized with yeast cell (c3bsyca) was used. the anti human c3 elisa was also used for 21 out of the 78 patients. the positive results in the entire group of the patients studied were found to reach 82% and the igg and igm cic were detected also with anti-human c3 elisa. the titres of cic thus determined decreased as the clinical conditions improved. it was ... | 1990 | 2390882 |
| occurrence of s-(1,2-dicarboxyethyl)-cysteine at position 77 in mutant human lysozyme secreted by saccharomyces cerevisiae. | a mutant human lysozyme p110, in which val110 was replaced with pro, was secreted by saccharomyces cerevisiae; modification of the cysteine residue at position 77 was found in a purified mutant protein (p110-b) upon primary structure analysis. a peptide fragment containing 15 amino acid residues from thr70 to leu84 was obtained by proteolytic digestion of the protein and subsequently isolated by reverse-phase hplc. this fragment was analyzed by high-resolution fast-atom-bombardment (fab) mass sp ... | 1990 | 2404763 |
| generation of deletion derivatives by targeted transformation of human-derived yeast artificial chromosomes. | mammalian dna segments cloned as yeast artificial chromosomes (yacs) can be manipulated by dna-mediated transformation when placed in an appropriate yeast genetic background. a "fragmenting vector" has been developed that can introduce a yeast telomere and selectable marker into human-derived yacs at specific sites by means of homologous recombination, deleting all sequences distal to the recombination site. a powerful application of the method uses a human alu family repeat sequence to target r ... | 1990 | 2406718 |
| protein n-myristoylation in escherichia coli: reconstitution of a eukaryotic protein modification in bacteria. | protein n-myristoylation refers to the covalent attachment of a myristoyl group (c14:0), via amide linkage, to the nh2-terminal glycine residue of certain cellular and viral proteins. myristoyl-coa:protein n-myristoyltransferase (nmt) catalyzes this cotranslational modification. we have developed a system for studying the substrate requirements and biological effects of protein n-myristoylation as well as nmt structure-activity relationships. expression of the yeast nmt1 gene in escherichia coli ... | 1990 | 2406721 |
| integration of heterologous genes into the chromosome of saccharomyces cerevisiae using a delta sequence of yeast retrotransposon ty. | distribution of a delta (delta) sequence of the ty element on a chromosome of the yeast saccharomyces cerevisiae was analysed by pulsed-field gel electrophoresis. more than 100 copies of the delta sequence were nonrandomly distributed on the chromosome. using the delta sequence as a recombination site, mouse alpha-amylase and human beta-endorphin genes were introduced into the chromosomal dna. the integration occurred on a particular chromosome in each case and the copy number was estimated as t ... | 1990 | 1369269 |
| progress toward the isolation and characterization of the genes causing neurofibromatosis. | neurofibromatosis 1 and neurofibromatosis 2 are clinically distinct autosomal dominant disorders that affect an estimated 1.5 million individuals throughout the world. the genetic defect in each disorder has been mapped to different chromosomes, nf1 to chromosome 17 and nf2 to chromosome 22. progress towards the cloning of the nf1 gene has proceeded rapidly. the nf1 locus was bracketed using genetic linkage analysis on nf1 affected pedigrees. physical mapping methods were then used to precisely ... | 1990 | 1669691 |
| cloning and characterisation of the saccharomyces cerevisiae glycerol-3-phosphate dehydrogenase (gut2) promoter. | the saccharomyces cerevisiae glycerol-3-phosphate dehydrogenase (gut2) promoter and part of the protein-coding region have been isolated on a 6.3-kb genomic dna fragment. nucleotide sequence analysis shows that the promoter has many structural features in common with yeast glycolytic enzyme promoters. chromosomal mapping indicates that this genomic fragment is located on chromosome xii. the gut2 promoter has been used to construct a recombinant human albumin (reha) secretion vector; yeast transf ... | 1991 | 1676389 |
| human vntr sequences in porcine htf-islands. | mapping of complex genomes has been influenced substantially by the isolation of locus-specific, but repetitive dna elements known as vntrs. since a high gc-content is characteristic of most of these elements one might expect them to be clustered at least partially in cpg-islands. to address this question we have constructed a porcine liver dna noti-linkage library in puc18 using isolated htf-islands. hpaii tiny fragments ranging from less than 100 bp to 1 kb in length were randomly selected and ... | 1991 | 1678358 |
| signal transduction and transcriptional regulation of interferon-alpha-stimulated genes. | interferon-alpha (ifn-alpha) stimulates the expression of a number of genes in a pathway that begins with binding to specific high-affinity plasma membrane receptors. all ifn-alpha-stimulated genes cloned thus far are characterized by the presence of a dna element, termed interferon-stimulated response element (isre), usually in the 5' upstream region of the genes. the isre binds a nuclear factor(s) following ifn-receptor triggered signal transduction and provides a convenient assay for the rapi ... | 1991 | 1680934 |
| synthesis of dna by dna polymerase epsilon in vitro. | the isolation of dna polymerase (pol) epsilon from extracts of hela cells is described. the final fractions contained two major subunits of 210 and 50 kda which cosedimented with pol epsilon activity, similar to those described previously (syvaoja, j., and linn, s. (1989) j. biol. chem. 264, 2489-2497). the properties of the human pol epsilon and the yeast pol epsilon were compared. both enzymes elongated singly primed single-stranded circular dna templates. yeast pol epsilon required the presen ... | 1991 | 1682323 |
| failure to detect integrins of the beta-2 subclass on the human sperm surface. | several cell-to-cell recognition systems utilize the tripeptide asp-gly-arg (rgd) as a cell surface signaling sequence. mac-1, a member of the integrin subfamily defined by the beta-2 subunit, recognizes c3bi by means of an rgd sequence. because c3 complement components have been detected on the gamete surfaces and rgd has been demonstrated to be involved in sperm-egg interaction, we explored the possibility that a member of the b2 integrin subfamily was present on the sperm surface and involved ... | 1991 | 1686173 |
| production of a site specifically cleavable p-glycoprotein-beta-galactosidase fusion protein. | we have fused full length and the carboxyl-half of human mdr1 cdna with the e. coli lacz gene via a collagen linker and allowed their expression in yeast saccharomyces cerevisiae. using antibodies against beta-galactosidase we partially purified the fusion proteins by immunoprecipitation and show here that the full length fusion protein has atpase activity. by contrast, the fusion protein containing the carboxyl-half of p-glycoprotein did not show atpase activity, indicating that both domains of ... | 1991 | 1369454 |
| expression of the human blood coagulation protein factor xiiia in saccharomyces cerevisiae: dependence of the expression levels from host-vector systems and medium conditions. | the human blood coagulation protein factor xiiia (fxiiia) was expressed in saccharomyces cerevisiae employing escherichia coli-yeast shuttle vectors based on a 2-mu plasmid. several factors affecting high production yield of recombinant fxiiia were analysed. the use of the regulatable gal-cyc1 hybrid promoter resulted in higher fxiiia expression when compared with the constitutive adci promoter. screening for suitable yeast strains for expression of fxiiia under the transcriptional control of th ... | 1991 | 1369455 |
| expression of human p-glycoprotein in yeast cells--effects of membrane component sterols on the activity of p-glycoprotein. | a human mdr1 cdna was introduced into yeast cells. immunoblot analysis and indirect immunostaining showed that some of the p-glycoprotein produced was situated in its native orientation in the yeast plasma membrane. drug-binding activities of the recombinant p-glycoproteins were markedly decreased compared to that of the authentic p-glycoprotein. to identify the bases of decreased binding we studied the effects of membrane component sterols on the azidopine binding and found that ergosterol, whi ... | 1991 | 1369463 |
| striking structural and functional similarities suggest that intestinal sucrase-isomaltase, human lysosomal alpha-glucosidase and schwanniomyces occidentalis glucoamylase are derived from a common ancestral gene. | sequence comparison of the primary structure of the yeast schwanniomyces occidentalis glucoamylase (gam) with gams in different microorganisms did not reveal significant similarities. by contrast, striking similarities were, surprisingly, found with 3 mammalian secretory and integral membrane proteins: the 2 subunits of intestinal brush border sucrase-isomaltase and human lysosomal alpha-glucosidase. the similarities among these proteins are found as clusters of up to 8 amino acids and distribut ... | 1991 | 1743281 |
| coactivators for a proline-rich activator purified from the multisubunit human tfiid complex. | the mechanisms of transcriptional activation directed by sequence-specific regulators is central to understanding gene regulation. here, we report the isolation of coactivators responsible for mediating transcriptional activation by gal4-pro, a hybrid regulator containing the proline-rich activation domain of human ctf/nfi. chromatographic studies indicate that endogenous human tfiid consists of a multisubunit complex containing the tata-binding protein (tbp), coactivators, and other associated ... | 1991 | 1748279 |
| purification, characterization, cloning, and amino acid sequence of the bifunctional enzyme 5,10-methylenetetrahydrofolate dehydrogenase/5,10-methenyltetrahydrofolate cyclohydrolase from escherichia coli. | we have purified the enzyme 5,10-methylenetetrahydrofolate dehydrogenase (ec 1.5.1.5) from escherichia coli to homogeneity by a newly devised procedure. the enzyme has been purified at least 2,000-fold in a 31% yield. the specific activity of the enzyme obtained is 7.4 times greater than any previous preparation from this source. the purified enzyme is specific for nadp. the protein also contains 5,10-methenyltetrahydrofolate cyclohydrolase (ec 3.5.4.9) activity. sodium dodecyl sulfate-polyacryl ... | 1991 | 1748668 |
| transfer of yeast artificial chromosomes from yeast to mammalian cells. | human dna can be cloned as yeast artificial chromosomes (yacs), each of which contains several hundred kilobases of human dna. this dna can be manipulated in the yeast host using homologous recombination and yeast selectable markers. in relatively few steps it is possible to make virtually any change in the cloned human dna from single base pair changes to deletions and insertions. in order to study the function of the cloned dna and the effects of the changes made in the yeast, the human dna mu ... | 1991 | 1755830 |
| dna sequences of chromosome 21-specific yac detect the t(8;21) breakpoint of acute myelogenous leukemia. | the t(8;21)(q22;q22) is a nonrandom translocation specifically marking blasts of acute myelogenous leukemia (aml) with undifferentiated phenotype. the breakpoint on chromosome 21 involved by this rearrangement has been precisely localized relative to cloned dna markers by physical and genetic linkage analysis enabling the use of positional cloning for its isolation. yeast artificial chromosome (yac) clones for loci proximal (d21s65) and distal (erg) to the (21q22) breakpoint have been developed ... | 1991 | 1756477 |
| [cryptococcal meningitis in aids: successful long-term prophylaxis with fluconazole]. | a 30-year-old, hiv-positive, man who had been repeatedly treated with amphotericin b for oral thrush, developed headaches, fever up to 38.5 degrees c, dizzy spells with falling tendency, as well as disorder of speech and word finding. cerebrospinal fluid (csf) contained 5700/3 cells, of which 90% were encapsulated yeast-fungus. cryptococcal antigen titres were elevated both in serum (1:256) and csf (1:1024), providing the diagnosis of cryptococcal meningitis. intravenous treatment was started wi ... | 1991 | 1756695 |
| variability in commercial histoplasma complement fixation antigens. | using immuno-mycologics (immy; norman, okla.) histoplasmal yeast (hy) and mycelial (hm) antibody complement fixation test antigens, we retested 1,386 samples that were initially tested with meridian diagnostics, inc. (cincinnati, ohio), antigens. histoplasma antibody was identified (greater than or equal to 1:16) in 20% of hy and 5% of hm samples reported to have titers of less than 1:8 with meridian reagents. immy titers were at least fourfold higher than meridian titers in 39% of hy and 54% of ... | 1991 | 1761695 |
| dna polymerase delta: gene sequences from plasmodium falciparum indicate that this enzyme is more highly conserved than dna polymerase alpha. | genes encoding proteins homologous to the catalytic subunits of dna polymerase alpha and delta have been cloned from the human malaria parasite plasmodium falciparum. these are among the first cellular replicative dna polymerase genes to be cloned and their sequences allow us to make new statements about the relative degrees of conservation of these two enzymes. the most important finding was that p. falciparum pol delta showed considerable homology to the only other pol delta enzyme for which p ... | 1991 | 1762904 |
| molecular linkage of the hla-dr, hla-dq, and hla-do genes in yeast artificial chromosomes. | eight major histocompatibility complex (mhc) class ii loci and the newly defined y3/ring 4 locus were isolated in overlapping yeast artificial chromosome (yac) clones defining a 420-kb segment of human chromosome 6p21.3. yac b1d12 spanning 320 kb contained seven of these loci from hla-dra to hla-dqb2. a 330-kb yac, a148a7, spanned from the hla-dqa1 locus through the y3/ring 4 locus and extended at least 130 kb centromeric of yac b1d12. southern blotting demonstrated that yac b1d12 derived from t ... | 1991 | 1774062 |
| application of an indirect immunofluorescence test for detection of mycoplasma pneumoniae in respiratory exudates. | we prepared polyclonal antibody specific to mycoplasma pneumoniae and examined the conditions influencing the ability of an indirect immunofluorescence test to detect the specific antigen in respiratory exudates. the antibody did not cross-react with normal human serum or with respiratory exudates from 10 healthy persons. cross-reactivity of the antibody with species of mycoplasmas other than m. genitalium was fully diminished when absorbed with horse serum and yeast extract, components of the c ... | 1991 | 1774328 |
| nucleotide sequence of a human heart cdna encoding the mitochondrial phosphate carrier. | we have isolated and characterized a full length cdna clone encoding the precursor of the human heart mitochondrial phosphate carrier protein. the entire clone is 1330 bp in length with 5'- and 3'-untranslated regions of 48 and 184 bp, respectively. the open reading frame encodes the mature protein consisting of 312 amino acids, preceded by a presequence of 49 amino acids. the amino acid sequence of the mature human phosphate carrier is 93.6, 94.2 and 33.6% identical to that of the phosphate car ... | 1991 | 1777677 |
| structure comparison and evolutionary relations between elongation factors ef-tu (ef-1 alpha) and sup 2 proteins. | on the basis of high homology and structural similarity, three genes, sup2 saccharomyces cerevisiae, sup2 pichia pinus and gst1 homo sapiens, might be considered as members of one family named sup2. comparison of the primary structure of sup2 proteins and elongation factors ef-tu(ef-1 alpha) from 19 different species was performed. it was found that sup2 proteins bear more homology to eukaryotic elongation factor than to procaryotic ef-tu, though the degree of sequence conservation in sup2 prote ... | 1991 | 1778473 |
| genetic and physical mapping around the properdin p gene. | a ca repeat has been found on the human x chromosome within 16 kb of the gene encoding properdin p factor (pfc) and has been shown to be a highly informative marker. two more polymorphic ca repeats were found in a cosmid containing dxs228. the ca repeats, and other markers from proximal xp, were mapped genetically in ceph families and the likely order of markers was established as xpter-(dxs7, mao-a, dxs228)-(pfc, dxs426)-(timp, oatl1)-dxs255-xcen. this places pfc in the region xp11.3-xp11.23, t ... | 1991 | 1783405 |
| [the occurrence of cryptococcus neoformans in fecal samples from birds kept in human living areas]. | with help of guizotia creatinine agar (syn. bird seed agar) cryptococcus (cr.) neoformans var. neoformans was isolated from 71 (7.7%) of 925 investigated droppings of birds kept within human living area. cr. neoformans was detected in droppings of 1.2% from 164 psittacines, in droppings of 1.7% from 118 budgerigars and in droppings of 18.4% from (carrier) pigeons. this yeast was not isolated from droppings of 13 small parrots, 10 eclectus parrots, 13 rosellas, 21 cockatiels, 6 polytelidus sp., 3 ... | 1991 | 1786044 |
| assessment of lymphocyte and phagocytic functions in goats treated with glucan. | the effect of glucan, a biological response modifier of yeast origin, on different immune functions was studied after the intramuscular application in goats. the simultaneous administration of glucan with human serum albumin or tetanus toxoid significantly stimulated the antibody production to both antigens differing in their thymus dependency. similarly, the phagocytizing activity of the blood polymorphonuclear leukocytes measured by the reductase colorimetric assay significantly increased one ... | 1991 | 1789024 |
| prohormone-converting enzymes: regulation and evaluation of function using antisense rna. | several putative peptide-processing endoproteases have been identified by homology to the yeast kex2 endoprotease, including furin, pc2, and pc1. however, the question is still open as to which might be involved in peptide posttranslational processing. to enable detailed comparisons of physiological changes in peptide processing with biochemical and molecular biological studies, we cloned rat pituitary cdnas for pc1 and pc2. the amino acid sequence homologies among rat, human, and mouse pc1, pc2 ... | 1991 | 1791845 |
| 1h and 15n nmr study of human lysozyme. | the 15n signal assignment of human lysozyme was carried out by using 1h-1h and 1h-15n two dimensional experiments. to solve the severe overlap problem of the nh signals, uniform labeling of the protein with 15n was introduced. the uniformly 15n labeled protein was prepared using a high-expression system of saccharomyces cerevisiae. from the analyses of 1h and 15n nmr spectra, all of the backbone 15n signals of the molecule were assigned to each specific residue in the amino acid sequence. recent ... | 1991 | 1794972 |
| stimulation of human neutrophil phagocytosis of candida albicans by a beta-glucan synthase inhibitor, cilofungin. | the effect of a lipopeptide antifungal agent, cilofungin, on serum opsonization and phagocytosis of candida albicans yeast phase cells in human neutrophil monolayer assays was investigated. simultaneous addition of fungicidal concentrations of cilofungin did not enhance or inhibit phagocytosis of c. albicans. pretreatment of candida blastospores with cilofungin in the absence of serum complement for 1 h did not affect phagocytosis. however, pretreatment of blastospores with cilofungin and comple ... | 1991 | 1797051 |
| generation of 5-fluorouracil from 5-fluorocytosine by monoclonal antibody-cytosine deaminase conjugates. | cytosine deaminase (cdase) catalyzes the conversion of cytosine to uracil and is also able to convert the clinically used antifungal agent 5-fluorocytosine (5fc) into the anticancer drug 5-fluorouracil (5fu). the enzyme was purified from bakers' yeast in a six-step procedure. studies indicated that bakers' yeast cdase had a molecular weight of approximately 32 kda and was composed of two subunits of equal molecular weights. monoclonal antibodies were covalently attached to cdase, forming conjuga ... | 1991 | 1805942 |
| [regulation of ca(2+)-dsrna for proliferation and terminal differentiation processes of human fibroblasts and hela cells]. | ca2+ complexes of dsrna, poly(da) and poly(dt) of yeast low molecular weight rna produce a pronounced mitogenic effect on human fibroblasts at early stages of fibroblast proliferation in culture. at later stages of cell cultivation ca(2+)-dsrna stimulates terminal differentiation by inducing the synthesis of proteins characteristic of the postmitotic population of human fibroblasts undergoing terminal differentiation. ca(2+)-dsrna produces a stimulating effect on c-fos and c-jun gene transcripti ... | 1991 | 1805979 |
| ultrastructural changes induced by alpha-sarcin in a human pulmonary tumor grown in naked mice. | alpha-sarcin is a cytotoxic polypeptide produced by aspergillus giganteus. it suppresses protein synthesis in yeast and wheat germ extracts and has a purine-specific rnase activity. the substance has been tested for its antitumor properties in a series of induced tumor systems in mice such as sarcoma and carcinoma among others. although some of the in vitro effects of alpha-sarcin on certain cellular components have been elucidated, the biological effects leading to cellular damage are still obs ... | 1991 | 1806047 |
| yeast artificial chromosome (yac) clones and sequence tagged site (sts)markers anchored at human chromosome 21. | 1991 | 1811099 | |
| partial purification of a mannosyltransferase involved in the o-mannosylation of glycoproteins from saccharomyces cerevisiae. | the mannosyltransferase that catalyses the transfer of mannose from dolichyl-phosphate-mannose (dol-p-man) to the hydroxyl group of serine/threonine residues in the acceptor peptide (tyr-asn-pro-thr-ser-val) was partially purified approximately 150-fold from the microsomal membrane fraction of saccharomyces cerevisiae. the membrane-bound enzyme was solubilized with 0.5% triton x-100 at a protein:detergent ratio of 2:1, and was then purified by ion-exchange chromatography on deae-cellulose, follo ... | 1991 | 1820198 |
| pas1, a yeast gene required for peroxisome biogenesis, encodes a member of a novel family of putative atpases. | pas genes are required for peroxisome biogenesis in the yeast s. cerevisiae. here we describe the cloning, sequencing, and characterization of the pas1 gene. its gene product, pas1p, has been identified as a rather hydrophilic 117 kd polypeptide. the predicted pas1p sequence contains two putative atp-binding sites and reveals a structural relationship to three other groups of proteins associated with different biological processes such as vesicle-mediated protein transport (nsf and sec18p), cont ... | 1991 | 1825027 |
| a novel cyclin encoded by a bcl1-linked candidate oncogene. | we have previously identified a candidate oncogene (prad1 or d11s287e) on chromosome 11q13 which is clonally rearranged with the parathyroid hormone locus in a subset of benign parathyroid tumours. we now report that a cloned human placental prad1 complementary dna encodes a protein of 295 amino acids with sequence similarities to the cyclins. cyclins can form a complex with and activate p34cdc2 protein kinase, thereby regulating progress through the cell cycle. prad 1 messenger rna levels vary ... | 1991 | 1826542 |
| annexin iii translocates to the periphagosomal region when neutrophils ingest opsonized yeast. | after phagocytosis, killing and digestion of ingested microorganisms depend on fusion of phagocytic vesicle membranes with membranes of intracellular vesicles (azurophil and specific granules). there is considerable evidence that phagosome-granule membrane fusion is regulated by transient increases in intracellular ionized ca2+. in previous studies, we found that a cytosolic ca2(+)-dependent membrane-binding protein, annexin iii, represents over 1% of the total protein of human neutrophils and p ... | 1991 | 1826705 |
| dephosphorylation and activation of a p34cdc2/cyclin b complex in vitro by human cdc25 protein. | oocytes arrested in the g2 phase of the cell cycle contain a p34cdc2/cyclin b complex which is kept in an inactive form by phosphorylation of its p34cdc2 subunit on tyrosine, threonine and perhaps serine residues. the phosphatase(s) involved in p34cdc2 dephosphorylation is unknown, but the product of the fission yeast cdc25+ gene, and its homologues in budding yeast and drosophila are probably positive regulators of the transition from g2 to m phase. we have purified the inactive p34cdc2/cyclin ... | 1991 | 1828290 |
| direct interaction between adenovirus e1a protein and the tata box binding transcription factor iid. | adenovirus e1a has long been known to activate/repress cellular and viral transcription. the transcriptional activity of nuclear extracts was depleted after chromatography on immobilized e1a protein columns that specifically retained the transcription factor (tf) iid. stronger direct interactions between e1a and human tfiid than between e1a and yeast tfiid suggest that the unique sequences of the human protein may be involved. we have demonstrated that this interaction occurs directly between ba ... | 1991 | 1828892 |
| both oct-1 and oct-2a contain domains which can activate the ubiquitously expressed u2 snrna genes. | the u2 snrna genes, which are transcribed by rna polymerase ii at high levels in all tissues examined, require both a distal and a proximal sequence element for efficient expression. the distal sequence element which has many properties in common with transcriptional enhancers contains, in addition to sp1 binding sites, an octamer binding site which mediates activation through interactions with the ubiquitous transcription factor oct-1. in the present study we have attempted to answer the questi ... | 1991 | 1829677 |
| enhanced phagocytosis and intracellular killing of pityrosporum ovale by human neutrophils after exposure to ketoconazole is correlated to changes of the yeast cell surface. | in seborrhoeic dermatitis an inflammatory response occurs secondary to large numbers of pityrosporum yeasts appearing within and beneath the epidermis. to study the interaction between human neutrophils and p. ovale and any immunomodulating effect of antifungal agents, the yeast was exposed to ketoconazole and then incorporated into neutrophil monolayer assays. phagocytosis was complement dependent and reached a maximum after 40 min. ketoconazole at 25, 50 and 100 mg l-1 had no significant effec ... | 1991 | 1833641 |
| cloning of all-1, the locus involved in leukemias with the t(4;11)(q21;q23), t(9;11)(p22;q23), and t(11;19)(q23;p13) chromosome translocations. | chromosomal region 11q23 participates in a number of reciprocal translocations with specific regions of chromosomes 4, 9, 19, and others. these translocations are associated with acute lymphocytic leukemia and acute myelomonocytic, monocytic, and myelogenous leukemia. from a yeast artificial chromosome containing human dna derived from 11q23 we cloned a dna fragment which can be used as a probe to detect rearrangements in leukemic cells from the majority of patients with the t(4;11), t(9;11), an ... | 1991 | 1835902 |
| mitogenic effect of lysosomal hydrolases on bovine tracheal myocytes in culture. | studies were conducted to assess the mitogenic effect of lysosomal hydrolases, enzymes known to have an association with allergen- or ozone-induced airway hyperreactivity, on bovine tracheal myocytes in culture. addition of purified human placental beta-hexosaminidase and partially purified bovine liver beta-glucuronidase resulted in the doubling of cell count after 4 d of incubation in medium m199 with 0.4% fbs. unstimulated cells remained quiescent without a significant increase of cell count. ... | 1991 | 1836469 |
| phosphorylation of replication protein a: a role for cdc2 kinase in g1/s regulation. | 1991 | 1840253 | |
| screening a yeast promoter library leads to the isolation of the rp29/l32 and snr17b/rpl37a divergent promoters and the discovery of a gene encoding ribosomal protein l37. | two promoters (a7 and a23), isolated at random from the saccharomyces cerevisiae genome by virtue of their capacity to activate transcription, are identical to known intergenic bidirectional promoters. sequence analysis of the genomic dna adjacent to the a7 promoter identified a split gene encoding ribosomal (r) protein l37, which is homologous to the trna-binding r-proteins, l35a (from human and rat) and l32 (from frogs). | 1991 | 1840541 |
| torulopsis candida (candida famata) endophthalmitis simulating propionibacterium acnes syndrome. | four months after undergoing extracapsular cataract extraction with implantation of a posterior chamber intraocular lens, a 74-year-old woman developed granulomatous anterior uveitis. although she initially responded well to corticosteroid therapy, she experienced multiple recurrences on discontinuation of this therapy. slit-lamp examination showed the ocular inflammation to be associated with white cortical material within the lens capsular sac. she underwent removal of the implant as well as t ... | 1991 | 1841584 |
| furin: the prototype mammalian subtilisin-like proprotein-processing enzyme. endoproteolytic cleavage at paired basic residues of proproteins of the eukaryotic secretory pathway. | furin, the translational product of the recently discovered fur gene, appears to be the first known mammalian member of the subtilisin family of serine proteases and the first known mammalian proprotein-processing enzyme with cleavage selectivity for paired basic amino acid residues. structurally and functionally, it resembles the prohormone-processing enzyme, kexin (ec 3.4.21.61), which is encoded by the kex2 gene of yeast saccharomyces cerevisiae. most likely, furin is primarily involved in th ... | 1991 | 1843280 |
| increase in the expression of a family of small guanosine triphosphate-binding proteins, rab proteins, during induced phagocyte differentiation. | rab is a newly identified family of small g-proteins that share 35-70% homology with the yeast sec4p and ypt1p involved in the regulation of the secretory pathway. mature phagocytes display functions requiring organized intracellular traffic and, for this reason, we questioned whether phagocyte differentiation could correlate with the increased expression of rab proteins. rabbit antisera raised against the recombinant proteins rab1ap, 2p, 4p, and 6p were able to detect the corresponding proteins ... | 1991 | 1847940 |
| expression of human papillomavirus proteins in yeast saccharomyces cerevisiae. | the l1 and l2 proteins of human papillomavirus (hpv) types 1, 6, and 16 and the e6 and e7 proteins of hpv 16 were expressed in saccharomyces cerevisiae. the yeast expressed proteins were readily detected by immune blotting and were generally intact. the hpv 1 l1 and l2 proteins expressed in yeast were indistinguishable from the major and minor capsid proteins purified from hpv 1 virions as judged by gel electrophoresis and immunoblotting. the hpv 6 and hpv 16 l2 proteins and hpv 16 e7 proteins w ... | 1991 | 1850917 |
| activation of the camp cascade by steroidogenic hormones and glucagon in the pathogenic fungus candida albicans. | incubation of candida albicans yeast cells with human luteinizing hormone (hlh), human chorionic gonadotrophin (hcg) or glucagon produced a significant rise in camp total levels. the effect of these hormones in permeabilized cells of the fungus produced a 2-3 fold increase in the mg2+, gtp-dependent adenylyl cyclase activity as well as full activation of the camp-dependent protein kinase (pka) activity. these results indicate that the interaction of the mammalian hormones with the fungus trigger ... | 1991 | 1851467 |
| chromatography of selenoproteins in human serum using matrix-bound heparin. | since previous experiments indicated that a major selenoprotein in human serum interacts with heparin, chromatography of serum on matrix-bound heparin was studied. when human serum was applied to heparin-agarose columns, approximately half of the applied selenium was not retained on the columns. approx. 40% of the selenium could then be eluted either with increasing concentrations of heparin or ammonium acetate. using a scaled-down version of this procedure, selenoproteins from 0.5 ml serum were ... | 1991 | 1856049 |
| phagocytosis of opsonized yeast induces tumor necrosis factor-alpha mrna accumulation and protein release by human polymorphonuclear leukocytes. | in this report we show that phagocytosis of yeast particles opsonized with igg (y-igg) by human polymorphonuclear cells (pmn) results in the selective induction of tumor necrosis factor (tnf-alpha) messenger rna (mrna) and release of its mature protein. lipopolysaccharide (lps) was also found able to induce tnf-alpha secretion by pmn, but was a less potent stimulus compared with y-igg. there was no evidence of interleukin-6 (il-6) gene expression in pmn after phagocytosis of y-igg or in response ... | 1991 | 1856593 |
| separation of human liver microsomal tolbutamide hydroxylase and (s)-mephenytoin 4'-hydroxylase cytochrome p-450 enzymes. | purification and immunoinhibition studies have suggested that the hydroxylations of (s)-mephenytoin and tolbutamide are catalyzed by rather similar forms of human liver cytochrome p-450 (p-450). however, the two activities are not well correlated in vivo; sulfaphenzaole is a selective inhibitor of tolbutamide hydroxylation, and expression of p-450 2c10 cdna in yeast yields a protein that hydroxylates tolbutamide but not (s)-mephenytoin. the p-450 2c8, 2c9, and 2c10 cdnas have all been isolated, ... | 1991 | 1857342 |
| characterization of a maize cdna that complements an enolase-deficient mutant of escherichia coli. | a cdna encoding maize enolase (2-phospho-d-glycerate hydrolase) was purified by functional genetic complementation using an enolase deficient mutant of escherichia coli, df261. this cdna, pzm245, was characterized by restriction mapping and dna sequence analysis. the cdna contained an open reading frame encoding a protein of 446 amino acids with a high degree of similarity to enolase sequences from other organisms (72% identity to yeast enolase and 82% identity to human enolase). the pzm245 cont ... | 1991 | 1859865 |
| cryosurvival of trichomonas vaginalis during cryopreservation of human semen. | despite a 90% cryosurvival of trichomonas vaginalis in their growth medium trypticase yeast maltose (tym) with dmso, none of these parasites have previously been observed to survive during cryopreservation of infected human semen with glycerol (andrologia 18, 323 (1986)). this could have been due to the failure of the culture method used to detect low numbers of survivors. the prospects of possible transmission of t. vaginalis by artificial insemination with cryobanked (-196 degrees c) semen pro ... | 1991 | 1864083 |
| phylogenetic analysis of five medically important candida species as deduced on the basis of small ribosomal subunit rna sequences. | the classification of species belonging to the genus candida berkhout is problematic. therefore, we have determined the small ribosomal subunit rna (srrna) sequences of the type strains of three human pathogenic candida species; candida krusei, c. lusitaniae and c. tropicalis. the srrna sequences were aligned with published eukaryotic srrna sequences and evolutionary trees were inferred using a matrix optimization method. an evolutionary tree comprising all available eukaryotic srrna sequences, ... | 1991 | 1865186 |
| a case of trichosporon pullulans infection of the lung with adult t-cell leukemia. | fungal infections are often reported, but trichosporon infection is very rare. a 78-year-old man with adult t-cell leukemia complicated with pulmonary infections is presented. bronchial exudate culture revealed many yeast-like colonies, which were morphologically and biochemically identified as trichosporon pullulans. | 1991 | 1865584 |
| analysis of yeast prp20 mutations and functional complementation by the human homologue rcc1, a protein involved in the control of chromosome condensation. | mutations in the prp20 gene of yeast show a pleiotropic phenotype, in which both mrna metabolism and nuclear structure are affected. srm1 mutants, defective in the same gene, influence the signal transduction pathway for the pheromone response. the yeast prp20/srm1 protein is highly homologous to the rcc1 protein of man, hamster and frog. in mammalian cells, this protein is a negative regulator for initiation of chromosome condensation. we report the analysis of two, independently isolated, rece ... | 1991 | 1865879 |
| human infection caused by exophiala pisciphila: case report and review. | one year after receiving a liver transplant and 2 months after treatment with high doses of steroids and monoclonal anti-cd3 for an episode of rejection, a 38-year-old woman developed a skin papule above the left medial malleolus. the papule, which at first had an annular shape, evolved into a pustule, ulcerated, drained, and assumed a crusted verrucous appearance. multiple satellite papules appeared around the lesion, which was incompletely excised and thought to represent squamous cell carcino ... | 1991 | 1866539 |
| secretion of n-glycosylated interleukin-1 beta in saccharomyces cerevisiae using a leader peptide from candida albicans. effect of n-linked glycosylation on biological activity. | human interleukin-1 beta (il-1 beta) is expressed in activated monocytes as a 31-kda precursor protein which is processed and secreted as a mature, unglycosylated 17-kda carboxyl-terminal fragment, despite the fact that it contains a potential n-linked glycosylation site near the nh2 terminus (-asn7-cys8-thr9-). cdna coding for authentic mature il-1 beta was fused to the signal sequence from the candida albicans glucoamylase gene, two amino acids downstream from the signal processing site. upon ... | 1991 | 1869556 |
| [1-14c]oleate-labeled autoclaved yeast: a membranous substrate for measuring phospholipase a2 activity in vitro. | radiolabeled, autoclaved yeast were tested as a substrate for mammalian phospholipase a2 activity because the only other membranous substrate used for this purpose, autoclaved escherichia coli, totally lacks a major mammalian phospholipid, phosphatidylcholine. candida albicans were grown in the presence of [1-14c]oleate and then autoclaved. sixty three percent of the incorporated label was in yeast phospholipid, and more than 95% of that was in the 2-acyl position. the distribution of label in t ... | 1991 | 1872466 |
| radiochemical assay of adenylosuccinase: demonstration of parallel loss of activity toward both adenylosuccinate and succinylaminoimidazole carboxamide ribotide in liver of patients with the enzyme defect. | a radiochemical assay for adenylosuccinase, an enzyme which intervenes twice in the biosynthesis of adenine nucleotides, has been developed. the two substrates of the enzyme, succinylaminoimidazole carboxamide ribotide (saicar) and adenylosuccinate (s-amp), were synthesized in radioactive form by incubating [2,3-14c]fumarate and, respectively, aicar and amp with partially purified adenylosuccinase from yeast. enzyme activities were determined by measuring the release of labeled fumarate after it ... | 1991 | 1872474 |
| a wild-type dna ligase i gene is expressed in bloom's syndrome cells. | alteration of dna ligase i activity is a consistent biochemical feature of bloom's syndrome (bs) cells. dna ligase i activity in bs cells either is reduced and abnormally thermolabile or is present in an anomalously dimeric form. to assess the role of dna ligase function in the etiology of bs, we have cloned the dna ligase i cdna from normal human cells by a pcr strategy using degenerate oligonucleotide primers based on conserved regions of the saccharomyces cerevisiae and schizosaccharomyces po ... | 1991 | 1881902 |
| sar1, a gene from schizosaccharomyces pombe encoding a protein that regulates ras1. | proper ras1 function is required for normal sexual function in the yeast schizosaccharomyces pombe. we have found a gene in s. pombe, sar1, that encodes a product capable of regulating ras1 function. sar1 is a member of an expanding family of ras gtpase-activating proteins (gaps) that includes mammalian gap, the yeast saccharomyces cerevisiae ira proteins, and the product of the human neurofibromatosis locus, nf1 sar1, like these other proteins, can complement the loss of ira function in s. cere ... | 1991 | 1883874 |
| human uracil-dna glycosylase complements e. coli ung mutants. | we have previously isolated a cdna encoding a human uracil-dna glycosylase which is closely related to the bacterial and yeast enzymes. in vitro expression of this cdna produced a protein with an apparent molecular weight of 34 k in agreement with the size predicted from the sequence data. the in vitro expressed protein exhibited uracil-dna glycosylase activity. the close resemblance between the human and the bacterial enzyme raised the possibility that the human enzyme may be able to complement ... | 1991 | 1886771 |
| mitochondrial activity and cytotoxicity of vitamin a (retinol) in yeast and human cell cultures. protective effect of antioxidants. | vitamin a inhibited the growth of yeast and human cells in a dose-dependent but selective manner in cultures utilizing a non-fermentable carbon and energy source. at sub-inhibitory concentrations in yeast cultures (approximately 100 micrograms/ml), the vitamin had a stimulatory effect on the mitochondrial system, foreshortening the lag phase in the adaptation to non-fermentable substrate. at inhibitory concentrations, vitamin a depressed mitochondrial protein synthesis relative to cytoplasmic pr ... | 1991 | 1888332 |
| rapid screening of a yac library by pulsed-field gel southern blot analysis of pooled yac clones. | a new method for screening of yac libraries is described. individual yacs were pooled into groups of 384 clones and prepared as samples suitable for pulsed-field gel electrophoresis. a five hit human yac library (brownstein et al., 1989) containing approximately 60,000 clones was condensed into 150 such pools and chromosomal dnas in each sample were separated on three pulsed field gels containing 50 samples each. southern blots prepared from these gels were hybridized with probes of interest to ... | 1991 | 1889812 |
| [therapy of hiv infection (aids)]. | the stages of human immunodeficiency viruses (hiv) life cycle are described as guide to therapeutic intervention. practical therapeutic recommendations are given. they should be directed to viruses as the causal agent and to the features of opportunistic infections as well as of associated malignant tumors. recently 3 progresses could be reached: (1) the application of azidothymidine in the latency phase, when the number of cd4 positive cells decreases below 500/mm3, whereby the progression of t ... | 1991 | 1897284 |
| molecular characterization of two galactosemia mutations: correlation of mutations with highly conserved domains in galactose-1-phosphate uridyl transferase. | galactosemia is an autosomal recessive disorder of human galactose metabolism caused by deficiency of the enzyme galactose-1-phosphate uridyl transferase (galt). the molecular basis of this disorder is at present not well understood. we report here two missense mutations which result in low or undetectable enzymatic activity. first, we identified at nucleotide 591 a transition which substitutes glutamine 188 by arginine. the mutated glutamine is not only highly conserved in evolution (conserved ... | 1991 | 1897530 |
| inhibition of intracellular growth of histoplasma capsulatum yeast cells by cytokine-activated human monocytes and macrophages. | human monocytes/macrophages (m psi) were infected with histoplasma capsulatum yeast cells, and intracellular growth was quantified after 24 h of incubation in medium alone or in medium containing cytokines. yeast cells multiplied within freshly isolated monocytes, cultured m psi, and alveolar m psi with intracellular generation times of 14.2 +/- 1.4, 18.5 +/- 2.1, and 19.9 +/- 1.9 h (mean +/- standard error of the mean), respectively. monocytes and m psi inhibited the intracellular growth of yea ... | 1991 | 1898916 |
| the yeast homolog to mouse tcp-1 affects microtubule-mediated processes. | a saccharomyces cerevisiae homolog to drosophila melanogaster and mouse tcp-1 encoding tailless complex polypeptide 1 (tcp1) has been identified, sequenced, and mapped. the mouse t complex has been under scrutiny for six decades because of its effects on embryogenesis and sperm differentiation and function. tcp1 is an essential gene in yeast cells and is located on chromosome 4r, linked to pet14. the tcp1-encoded proteins in yeast, drosophila, and mouse cells share between 61 and 72% amino acid ... | 1991 | 1901944 |
| analyzing the substrate specificity of saccharomyces cerevisiae myristoyl-coa:protein n-myristoyltransferase by co-expressing it with mammalian g protein alpha subunits in escherichia coli. | a dual plasmid system was used to examine the protein and acyl-coa specificities of saccharomyces cerevisiae myristoyl-coa:protein n-myristoyltransferase (nmt) by co-expressing it in escherichia coli with each of four homologous alpha subunits of the signal-transducing, heterotrimeric g proteins. exogenous [3h]myristate was incorporated into rat gi alpha 1 and rat go alpha but not into bovine gs alpha or human gz alpha. oxygen for methylene group substitutions in myristate result in analogs with ... | 1991 | 1903791 |
| sera from patients with colon, breast and lung cancer induce resistance to lysis mediated by nk cytotoxic factors (nkcf). | natural killer (nk) cells are involved in the antitumoral immunologic mechanism. these cells act through the release of cytotoxic molecules defined as nk cytotoxic factors (nkcf). inhibitory factors of nk and nkcf mediated lysis have been described in in vitro assays. this study evaluates the induction of resistance to nkcf cytotoxicity by sera from 27 patients with colon, breast and lung cancer. addition of these sera to the cytolytic assay where k562 cells and concentrated nkcf were used, indu ... | 1991 | 1906292 |
| resolution of a low molecular weight g protein in neutrophil cytosol required for nadph oxidase activation and reconstitution by recombinant krev-1 protein. | activation of the membrane-associated nadph oxidase in intact human neutrophils requires a receptor-associated heterotrimeric gtp-binding protein that is sensitive to pertussis toxin. activation of this nadph oxidase by arachidonate in a cell-free system requires an additional downstream pertussis toxin-insensitive g protein (gabig, t. g., english, d., akard, l. p., and schell, m. j. (1987) (j. biol. chem. 262, 1685-1690) that is located in the cytosolic fraction of unstimulated cells (gabig, t. ... | 1991 | 1906890 |
| assessment of the transcriptional activation potential of the hmg chromosomal proteins. | chromosomal proteins hmg-14, hmg-17, and hmg-1 are among the most abundant, ubiquitous, and evolutionarily conserved nonhistone proteins. analysis of their structure reveals features which are similar to those of certain transcription factors. the distribution of charged amino acid residues along the polypeptide chains is asymmetric: positive charges are clustered toward the n-terminal region, while negative charges are clustered toward the c-terminal region. the residues in the c-terminal regio ... | 1991 | 1908554 |
| rsr1 and rap1 gtpases are activated by the same gtpase-activating protein and require threonine 65 for their activation. | the rsr1 protein of saccharomyces cerevisiae has been shown to be essential for bud site selection (bender, a., and pringle, j. (1989) proc. natl. acad. sci. u.s.a. 86, 9976-9980). this protein of 272 amino acids shares approximately 50% sequence identity with both ras and rap gtpases. however, neither gtp binding nor gtpase activity of the rsr1 protein has been reported. the rsr1 protein shares with human rap1 gtpases the four specific motifs, i.e. gly-12, residues 32-40, ala-59, and residues 6 ... | 1991 | 1910037 |
| why yeast? | yeast is an apparently strange "model" for the human genome, but it works because of the high degree of conservation in evolution between the primitive eukaryote and mammals. moreover, the genic concentration in yeast--with almost no noncoding introns--packs the entire yeast genome into 16 chromosomes with only 10% of the dna of one human chromosome. | 1991 | 1918194 |
| [mass spectrometric structure analysis of fatty acid mixtures from biological material after gas capillary chromatographical separation]. | the identification of mixtures of fatty acids from biological materials is possible by electron impact ionization mass spectra of methyl esters after their capillary gas chromatographic separation. mass spectra of pyrrolidine derivatives are used for the determination of double bond positions in unsaturated fatty acids. 97 different fatty acids (saturated, unsaturated, branched, cyclic, hydroxy, oxo, epoxy and methoxy) and other compounds (alkanes, halogens, phthalates, ketones, aldehydes) were ... | 1991 | 1922276 |
| differential expression of two types of the neurofibromatosis type 1 (nf1) gene transcripts related to neuronal differentiation. | a 360 residue region encoded by the neurofibromatosis type 1 (nf1) gene shows significant homology to the catalytic domains of both mammalian gtpase-activating proteins (gap) and yeast ira proteins. this gap-related domain of the nf1 gene (nf1-grd), like the gap and ira protein, has been reported to mediate hydrolysis of ras-bound gtp to gdp, resulting in inactivation of ras protein. in the present study, we identified two different types of nf1-grd cdna. one (type i) is identical to the previou ... | 1991 | 1923522 |
| a rat brain mrna encoding a transcriptional activator homologous to the dna binding domain of retroviral integrases. | we have isolated a rat cdna, named fe65, hybridizing to an mrna of about 2,300 nucleotides present in rat brain, undetectable in rat liver and very poorly represented in other tissues. an mrna of the same size is present in human neuroblastoma cells and is absent from other human cell lines. the fe65 cdna contains an open reading frame (orf) coding for a polypeptide of 499 amino acids in which 143 residues can be aligned with the dna binding domain of the integrases encoded by mammalian immunode ... | 1991 | 1923810 |
| the human homologous pairing protein hpp-1 is specifically stimulated by the cognate single-stranded binding protein hrp-a. | homologous pairing and strand exchange of dna are catalyzed by the human homologous pairing protein hpp-1 in a magnesium-dependent, atp-independent reaction that requires homologous dna substrates and stoichiometric quantities of hpp-1. here we show that the addition of the purified human single-strand binding (ssb) protein hrp-a to the reaction mixture stimulates the rate of homologous pairing 70-fold and reduces the amount of hpp-1 required for the reaction at least 10-fold. the identification ... | 1991 | 1924369 |
| cloning and characterization of a 3-methyladenine dna glycosylase cdna from human cells whose gene maps to chromosome 16. | we described previously the isolation of a saccharomyces cerevisiae 3-methyladenine (3-meade) dna glycosylase repair gene (mag) by its expression in glycosylase-deficient escherichia coli alka tag mutant cells and its ability to rescue these cells from the toxic effects of alkylating agents. here we extend this cross-species functional complementation approach to the isolation of a full-length human 3-meade dna glycosylase cdna that rescues alka tag e. coli from killing by methyl methanesulfonat ... | 1991 | 1924375 |
| sequence requirements for proteolytic cleavage of precursors with paired basic amino acids. | when expressed in cos cells, human prorenin was secreted into the medium without being processed to an active renin. co-expression of furin, a mammalian homologue of the yeast kex2 gene product, did not affect proteolytic processing of prorenin. a mutant proreninr-4 constructed by site-directed mutagenesis of pro (-4) to arg was not cleaved by an endoprotease in the cos cell. however, proreninr-4 was detectably cleaved to yield the active renin upon co-transfection with furin dna, indicating tha ... | 1991 | 1930163 |
| phosphoglucoisomerase-catalyzed interconversion of hexose phosphates. a model for d-[2-3h]glucose metabolism in human erythrocytes. | when d-[2-3h]glucose 6-phosphate mixed with the unlabeled ester is converted to d-[1-3h]fructose 6-phosphate and 3hoh in the phosphoglucoisomerase reaction and then to d-[1-3h]fructose 1,6-bisphosphate in the phosphofructokinase reaction, the specific radioactivity of the latter metabolite and the production of 3hoh relative to the total generation of tritiated end products are both inversely related to the concentration of phosphofructokinase. in human erythrocytes, the modeling of d-[2-3h]gluc ... | 1991 | 1931158 |
| human recombinant granulocyte-macrophage colony-stimulating factor augments viability and cytotoxic activities of human monocyte-derived macrophages in long-term cultures. | in the present study we investigated the effect of human recombinant granulocyte-macrophage csf (gm-csf) on the in vitro maturation of human monocytes into macrophages, and followed the biochemical and functional changes in the cells during this process. adherent human peripheral blood monocytes cultured for up to 3 weeks in the presence of gm-csf were examined for viability and adherence, beta-glucosaminidase activity, oxidative burst activity, expression of fucosyl-mannosyl receptors, and tnf- ... | 1991 | 1932369 |
| low-usage codons in escherichia coli, yeast, fruit fly and primates. | codon usage is compared between four classes of species, with an emphasis on characterization of low-usage codons. the classes of species analyzed include the bacterium escherichia coli (eco), the yeast saccharomyces cerevisiae (ysc), the fruit fly drosophila melanogaster (dro), and several species of primates (pri) (taken as a group; includes eleven species for which nucleotide sequence data have been reported to genbank, however, greater than 90% of the sequences were from homo sapiens). the n ... | 1991 | 1937008 |
| alteration of n-terminal residues of mature human lysozyme affects its secretion in yeast and translocation into canine microsomal vesicles. | signal sequences play a central role in the initial membrane translocation of secretory proteins. their functions depend on factors such as hydrophobicity and conformation of the signal sequences themselves. however, some characteristics of mature proteins, especially those of the n-terminal region, might also affect the function of the signal sequences. to examine this possibility, several mutants of human lysozyme modified in the n-terminal region of the mature protein were constructed, and th ... | 1991 | 1939091 |
| catalysis of guanine nucleotide exchange on ran by the mitotic regulator rcc1. | the product of the gene rcc1 (regulator of chromosome condensation) in a bhk cell line is involved in the control of mitotic events. homologous genes have been found in xenopus, drosophila and yeast. a human genomic dna fragment and complementary dna that complement a temperature-sensitive mutation of rcc1 in bhk21 cells encode a protein of relative molecular mass 45,000 (mr 45k) which is located in the nucleus and binds to chromatin. we have recently isolated a protein from hela cells that stro ... | 1991 | 1944575 |
| ocular histoplasmosis with retinitis in a patient with acquired immune deficiency syndrome. | disseminated histoplasmosis is one of the life-threatening opportunistic infections associated with acquired immune deficiency syndrome (aids). a 29-year-old man with aids and disseminated histoplasmosis complained of a hazy spot in the vision of his left eye. results of examination showed distinct creamy white intraretinal and subretinal infiltrates in both eyes. the patient died within a month from pulmonary infection with histoplasma capsulatum and cytomegalovirus. examination with light micr ... | 1991 | 1945308 |