Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| association of the presence of helicobacter in gallbladder tissue with cholelithiasis and cholecystitis. | the presence of helicobacter dna species has been investigated in the biliary epithelium of patients with biliary diseases. however, conflicting results have been observed that may have been due to the small number of subjects studied, difficulty in obtaining a healthy control group, absence of controlling for confounding factors, or differences among populations. therefore, we investigated the presence of helicobacter species by culture and nested pcr of 16s rrna genes in gallbladder tissue and ... | 2003 | 14662950 |
| expression of cd3 epsilon subunit in gastric parietal cells: a possible role in signal transduction? | cd3 antigen, formerly thought to be specific for t lymphocytes, has been found on gastric parietal cells in animals and humans. the common anti-cd3 antibodies recognize the epsilon subunit, which has a role in signal transduction. the aim of this study was to immunostain stomach specimens from humans and different animal species for cd3 antigen to determine if cd3 antigen is conserved across species and if cd3 antigen expression is altered in humans by use of certain drugs or the presence of gas ... | 2003 | 12812314 |
| molecular mimicry in atherosclerosis: a role for heat shock proteins in immunisation. | atherosclerosis has long been recognised as having an inflammatory component, and this has a particularly important bearing on to its clinical complications as it may result in plaque instability. results of recent epidemiological studies have reinforced the potential importance of this aspect of the disease. positive associations have been reported between exposure to several specific pathogens, and future risk of coronary heart disease (chd). whilst it is possible that each individual organism ... | 2003 | 12818399 |
| gastric transitional zones, areas where helicobacter treatment fails: results of a treatment trial using the sydney strain mouse model. | current combination therapies cure helicobacter pylori infection in 75 to 85% of cases. however, many treatment failures are not explained by antibiotic resistance. our goal was to explore treatment failures under in vivo conditions by using the h. pylori sydney strain (ss1) mouse model. mice infected with h. pylori (ss1) were treated with monotherapies or combination therapies used in human trials. bacterial levels and distribution of organisms within the stomach were assessed 24 h after treatm ... | 2003 | 12821476 |
| [chronic urticaria and infectious diseases]. | infectious diseases are often considered as a classic cause of chronic urticaria. nevertheless, laboratory investigations greatly vary from one centre to the other and the link between the infection and skin signs does not rely on hard data. the purpose of this work was a systematic analysis of the published cases of urticaria associated with infection. | 2003 | 12843809 |
| idiopathic interstitial pneumonia following stem cell transplantation. | idiopathic interstitial pneumonia (iip) can occur after stem cell transplantation, but the aetiology is unknown. based on the association between angiitis syndrome and helicobacter pylori infection, we identified possible risk factors common to these two conditions. among 83 patients who underwent stem cell transplantation, four developed iip. we elucidated various parameters and clinical features in four patients with iip and 79 patients without, after allogeneic stem cell transplantation. in a ... | 2003 | 12868990 |
| occurrence of a nontypable helicobacter pylori strain lacking lewis blood group o antigens and dd-heptoglycan: evidence for the role of the core alpha1,6-glucan chain in colonization. | the cell envelope of helicobacter pylori contains a lipopolysaccharide (lps) essential for the physical integrity and functioning of the bacterial cell membrane. the o-chain of this lps frequently expresses type 2 lewis x (lex) and lewis y (ley) blood group antigens that mimic human gastric mucosal cell-surface glycoconjugates. this article describes the isolation and structural analysis of the lps from a clinical isolate of h. pylori strain pj2 that lacks le antigens but is still capable of col ... | 2003 | 12907691 |
| recent advances in helicobacter pylori infection in children: from the petri dish to the playground. | helicobacter pylori infection is acquired in childhood, plays a causative role in chronic gastritis and peptic ulcer disease, and is associated with the development of gastric cancer. the present review focuses on recent advances in the scientific knowledge of h pylori infection in children, including clinical sequelae, diagnosis and treatment. in addition, recent insights regarding both bacterial and host factors that mediate human diseases associated with h pylori infection are discussed. | 2003 | 12915920 |
| human leukocyte antigen class ii genes and helicobacter pylori infection: does genotype overwhelm environmental exposure? | we investigated associations between human leukocyte antigen class ii genes, environmental exposures, and helicobacter pylori infection. | 2003 | 12921878 |
| il-1 rn 2/2 genotype and simultaneous carriage of genotypes il-1 rn 2/2 and il-1beta-511 t/t associated with oesophagitis in helicobacter pylori-negative patients. | interleukin-1 receptor antagonist genotype 2/2 is associated with a prolonged and enhanced inflammatory response. it is suspected of being a risk factor for atrophic gastritis and gastric cancer and for some autoimmune diseases. no specific genetic risk factors for oesophagitis have been identified so far and there are no reports of il-1 polymorphism in relation to oesophageal disease. | 2003 | 14750640 |
| review article: clinical significance of mucosal-protective agents: acid, inflammation, carcinogenesis and rebamipide. | while a great deal of clinical evidence has been found regarding anti-acids for the treatment of gastric disorders including peptic ulcers, not all disorders can be explained only by the hyperfunction of acid secretion. especially in the asian region, glandular atrophy is more prominent than in western countries, therefore low acid output is often observed in these patients. improvement of mucosal protection is rational therapy for these patients; this is the reason for use of these agents in as ... | 2003 | 12925154 |
| treatment of helicobacter pylori infection using a novel antiadhesion compound (3'sialyllactose sodium salt). a double blind, placebo-controlled clinical study. | 3' sialyllactose sodium salt (3'sl) is an oligosaccharide that occurs naturally in human and bovine milk. it can inhibit the adhesion of h. pylori to human epithelial cells in vitro. the aim of this study was to test whether this oligosaccharide can suppress or cure h. pylori colonization in vivo and to determine its safety in humans. | 2003 | 12950597 |
| cytoskeletal rearrangements in gastric epithelial cells in response to helicobacter pylori infection. | helicobacter pylori causes host epithelial cell cytoskeletal rearrangements mediated by the translocation and tyrosine phosphorylation of an outer-membrane protein, caga, and by the vacuolating cytotoxin, vaca. however, the mechanisms by which h. pylori mediates cytoskeletal rearrangements in infected host cells need to be more clearly defined. the aim of this study was to determine the effects of h. pylori isolates from children on the architecture of host gastric epithelial cells. gastric epit ... | 2003 | 12972579 |
| acid-adaptive genes of helicobacter pylori. | helicobacter pylori is the only neutralophile that has been able to colonize the human stomach by using a variety of acid-adaptive mechanisms. one of the adaptive mechanisms is increased buffering due to expression of an acid-activated inner membrane urea channel, urei, and a neutral ph-optimum intrabacterial urease. to delineate other possible adaptive mechanisms, changes in gene expression in response to acid exposure were examined using genomic microarrays of h. pylori exposed to different le ... | 2003 | 14500513 |
| cutting edge: cyclooxygenase-2 activation suppresses th1 polarization in response to helicobacter pylori. | helicobacter pylori infection causes a th1-driven mucosal immune response. cyclooxygenase (cox)-2 is up-regulated in lamina propria mononuclear cells in h. pylori gastritis. because cox-2 can modulate th1/th2 balance, we determined whether h. pylori activates cox-2 in human pbmcs, and the effect on cytokine and proliferative responses. there was significant up-regulation of cox-2 mrna and pge(2) release in response to h. pylori preparations. addition of cox-2 inhibitors or an anti-pge(2) ab resu ... | 2003 | 14530307 |
| the muc5ac glycoprotein is the primary receptor for helicobacter pylori in the human stomach. | helicobacter pylori shows a characteristic tropism for the mucus-producing gastric epithelium. in infected patients, h. pylori colocalizes in situ with the gastric secretory mucin muc5ac. the carbohydrate blood-group antigen lewis b (leb) was deemed responsible for the adherence of h. pylori to the gastric surface epithelium. we sought to determine if muc5ac is the carrier of leb, and thus if muc5ac is the underlying gene product functioning as the main receptor for h. pylori in the stomach. | 2003 | 14535999 |
| inhibition of helicobacter pylori-induced nuclear factor-kappa b activation and interleukin-8 gene expression by ecabet sodium in gastric epithelial cells. | helicobacter pylori stimulates nuclear factor-kappa b (nf-kappa b) activation and chemokine interleukin-8 (il-8) expression in gastric epithelial cells. ecabet sodium (ecabet), a locally acting antiulcer drug, is known to have anti-h. pylori activity. however, there is little understanding of how ecabet induces anti-inflammatory activity in gastric epithelial cells infected with h. pylori. the aim of this study was to investigate the effects of ecabet on il-8 gene expression and nf-kappa b activ ... | 2003 | 14536001 |
| [relationship between helicobacter pylori infection and expression of c-myc, bcl-2, and bax protein in different gastric mucosa lesions]. | helicobacter pylori (hp) has been believed to be a carcinogen of gastric carcinoma. however, its mechanism was yet not clearly understood. this study was designed to investigate the relationship between hp infection and gastric epithelial cell proliferation as well as apoptosis in different gastric mucosa lesions and elucidate the probable mechanism of gastric carcinogenesis relating with hp infection. | 2003 | 14558945 |
| [disturbance of intracellular signaling by helicobacter pylori infection]. | 2003 | 14577356 | |
| remission of severe anemia persisting for over 20 years after eradication of helicobacter pylori in cases of ménètrier's disease and atrophic gastritis: helicobacter pylori as a pathogenic factor in iron-deficiency anemia. | a man with a 20-year history of recurrent iron-deficiency anemia complicated by helicobacter pylori-positive ménètrier's disease was observed over a 10-year clinical course, during which time he was successfully treated for the anemia and a gastric helicobacter pylori (h. pylori) infection through eradication. considering the satisfactory therapeutic results in this case, we performed eradication therapy on another h. pylori-positive atrophic gastritis case with a 24-year history of iron-deficie ... | 2003 | 14606710 |
| micropneumatosis coexistent with helicobacter pylori and its improvement. | micropneumatosis intestinalis is the occurrence of gas-filled circular voids with diameter of 20-200 microns, not lined with epithelium. we report a 39-year-old man with superficial gastritis and helicobacter pylori infection who also had gastric, duodenal and colonic micropneumatosis. endoscopic biopsy after treatment for h. pylori gastritis showed no micropneumatosis in gastric, duodenal or colonic mucosal sections. we suggest that h. pylori may be one of the causative factors for micropneumat ... | 2003 | 14658539 |
| api2-malt1 fusion gene in colorectal lymphoma. | the api2-malt1 fusion gene was originally identified from a t(11;18)(q21;q21) translocation, a specific chromosomal abnormality that is found in mucosa-associated lymphoid tissue (malt) lymphoma. gastric malt lymphomas positive for the api2-malt1 fusion gene do not respond to helicobacter pylori-eradication therapy, but otherwise, the incidence and clinicopathological behavior of colorectal malt lymphoma with this genetic abnormality are unclear. we examined the api2-malt1 fusion by multiplex rt ... | 2003 | 14681324 |
| effect of helicobacter pylori eradicated therapy on water gastric emptying in patients with active duodenal ulcer. | it remains debatable if duodenal ulcer (du) or helicobacter pylori infection has a definite impact on human gastric emptying (ge). we explored the nature of water ge in active du patients before and after ulcer healing and the influence of h. pylori eradication on ge. | 2003 | 14535981 |
| evaluation of helicobacter species in inflammatory bowel disease. | bacteria have been implicated in the pathogenesis of inflammatory bowel disease. helicobacter species have been shown to cause colitis in animal models and have been identified in human diarrhoeal illness and crohn's disease. | 2003 | 12950420 |
| coexpression of helicobacter pylori's proteins caga and hspb induces cell proliferation in ags gastric epithelial cells, independently from the bacterial infection. | adenocarcinoma of the stomach is the second most common cause of cancer mortality in the world. the purpose of this study was to evaluate the potential role in carcinogenesis of two secreted helicobacter pylori's proteins, caga and hspb, both shown to increase the risk of gastric carcinoma in patients infected with h. pylori-positive strain. the effects of these two proteins on cell kinetics and the ability to selectively affect the expression of cell cycle-related proteins by transfection of a ... | 2003 | 14559823 |
| helicobacter pylori-induced homotypic phagosome fusion in human monocytes is independent of the bacterial vaca and cag status. | following reports that a vaca+cag+ toxigenic but not a vaca-cag- non-toxigenic helicobacter pylori strain induced homotypic phagosome fusion in murine macrophages, we addressed that phenomenon in human cells. mononuclear phagocytes and epitheloid cells were challenged with h. pylori strains of different vaca and cag genotypes and with vaca- and cag- isogenic mutants, and chased in the absence or presence of signal transduction modulators. electron microscopy revealed that, in monocytes: (i) homo ... | 2003 | 14641174 |
| effect of omeprazole 10 mg on intragastric ph in three different cyp2c19 genotypes, compared with omeprazole 20 mg and lafutidine 20 mg, a new h2-receptor antagonist. | omeprazole 10 mg is used as maintenance therapy for gastro-oesophageal reflux disease, but previous reports have not mentioned the potency of its acid suppression. | 2003 | 14653835 |
| expression of heat shock protein 32 (hemoxygenase-1) in the normal and inflamed human stomach and colon: an immunohistochemical study. | heat shock protein 32 (hsp32, hemoxygenase-1) is induced by reactive oxygen metabolites (rom) and degrades heme leading to the formation of antioxidant bilirubin. increased mucosal generation of rom occurs in gastritis and inflammatory bowel disease. we aimed to assess mucosal expression of hsp32 in normal stomach and colon and to test the hypothesis that disease-related differential expression occurs in inflamed tissue. gastric body and antral mucosal biopsies were obtained from 33 patients com ... | 2003 | 15115285 |
| high-dose rabeprazole/amoxicillin therapy as the second-line regimen after failure to eradicate h. pylori by triple therapy with the usual doses of a proton pump inhibitor, clarithromycin and amoxicillin. | some patients are refractory to the usual triple therapy for eradication of helicobacter pylori, consisting of a proton pump inhibitor, amoxicillin and clarithromycin, so there needs to be an alternative strategy for retreatment after failure to eradicate the infection. | 2003 | 14696516 |
| extracts of spice and food plants from thai traditional medicine inhibit the growth of the human carcinogen helicobacter pylori. | helicobacter pylori (hp) is a gramnegative bacterium and well recognized as being the primary etiological agent responsible for the development of gastritis, dyspepsia, peptic ulcer disease and gastric cancer. in developing countries, a high prevalence of hp infection is associated with an increased incidence of gastric cancer. thailand, however, while having a high prevalence of hp infections, has a lower than expected gastric cancer rate than other developing countries. it has been suggested t ... | 2003 | 14758718 |
| expression of nuclear factor-kappa b and target genes in gastric precancerous lesions and adenocarcinoma: association with helicobactor pylori caga (+) infection. | to examine the expression of nuclear factor kappab (nf-kappab) and its target genes in intestinal metaplasia (im), dysplasia (dys) and gastric carcinoma (gc) infected with helicobacter pylori (h pylori) and to investigate the mechanism underlying h pylori cytotoxin associated gene a (cag a) infection leading to gastric adenocarcinoma. | 2004 | 14966904 |
| [are cox-2 inhibitors truly able to prevent nsaids-associated ulcer?]. | non-steroidal anti-inflammatory drugs(nsaids) have a potential to cause mucosal injury in the gastrointestinal tract. inhibitions of cyclooxygenase, one of the targets of nsaids and direct cytotoxic effects of nsaids, are reported to be involved in nsaids-related mucosal damage. it is estimated that 15-30% of patients taking nsaids develop gastroduodenal ulcers, 2% of patients have life-threatening complications. normal gastroduodenal mucosa expresses only cox-1, and it is reported that nsaids a ... | 2004 | 15038104 |
| receptor-mediated targeting of lipobeads bearing acetohydroxamic acid for eradication of helicobacter pylori. | in the present context, phosphatidyl ethanolamine (pe) liposomes anchored polyvinyl alcohol (pva) xerogel beads (lipobeads) bearing acetohydroxamic acid (aha) was developed as a receptor-mediated drug delivery system for use in blocking adhesion of helicobacter pylori and thereby preventing the sequelae of chronic gastric infections. pva beads containing aha were prepared by emulsification followed by low temperature crystallization method. surface acylation with fatty acid chain was accomplishe ... | 2004 | 15342178 |
| helicobacter pylori-mediated gastritis induces local downregulation of secretory leukocyte protease inhibitor in the antrum. | helicobacter pylori-infected subjects exhibited a strong decline in antral secretory leukocyte protease inhibitor (slpi) levels compared to h. pylori-negative subjects and subjects from whom h. pylori had been eradicated (p = 0.002). this reduction was specific for the antrum, whereas slpi expression in corpus and duodenum was not affected. antral slpi levels were inversely correlated with inflammatory scores of antrum-predominant gastritis. | 2004 | 15039364 |
| the future of gastric cancer prevention. | despite advances in surgical treatment and chemotherapy, gastric cancer remains a major global health burden. the most recent estimates show that it is the fourth most common cancer and the second most common cause of cancer deaths worldwide. various etiologic factors have been linked with the disease. it is widely accepted that helicobacter pylori infection and high salt intake are positively associated with this neoplastic process. controversial associations have been found with smoking or dri ... | 2004 | 15052434 |
| genetic pathways of two types of gastric cancer. | multiple genetic and epigenetic alterations in oncogenes, tumour-suppressor genes, cell-cycle regulators, cell adhesion molecules, dna repair genes and genetic instability as well as telomerase activation are implicated in the multistep process of human stomach carcinogenesis. however, particular combinations of these alterations differ in the two histological types of gastric cancer, indicating that well-differentiated or intestinal-type and poorly differentiated or diffuse-type carcinomas have ... | 2004 | 15055305 |
| helicobacter pylori associated antigastric autoantibodies: role in sjögren's syndrome gastritis. | previous studies have shown that helicobacter pylori seroprevalence in sjögren's syndrome is comparable with that of the general population. however, the origin of the chronic gastropathy associated with this syndrome and the role of local autoimmunity--possibly triggered by bacterial infection--in its pathogenesis remain unclear. | 2004 | 15156903 |
| revisiting the pestilence of helicobacter pylori: insights into geographical genomics and pathogen evolution. | helicobacter pylori causes chronic gastritis and plays important roles in peptic ulcer disease, gastric carcinoma, and mucosa-associated lymphoid tissue (malt) lymphoma. it is believed that h. pylori infects over 50% of the worlds' population. however, only a small subset of infected people experience h. pylori-associated illnesses. associations with disease-specific factors remain enigmatic. the contribution of comparative genomics to our understanding of the genome organisation and diversity o ... | 2004 | 15157625 |
| cathepsins k, l, b, x and w are differentially expressed in normal and chronically inflamed gastric mucosa. | the expression of cathepsins k, l, b, x and w was studied by quantitative rt-pcr in normal and inflamed gastric mucosa (antrum, corpus, cardia). cathepsins b, l, k and x were expressed ubiquitously. in contrast, cathepsin w was expressed at very low levels. infection by helicobacter pylori caused a significant induction of cathepsin x (p<0.008), whereas the other cathepsins were not or only locally affected by h. pylori infection or reflux disease. immunohistochemistry revealed specific expressi ... | 2004 | 15196006 |
| gastric autoimmunity: the role of helicobacter pylori and molecular mimicry. | pathogens can induce autoreactive t cells to initiate autoimmune disease by several mechanisms. pathogen-induced inflammation results in the enhanced presentation of self antigens, which causes the expansion of the activated autoreactive t cells that are required for disease onset. alternatively, a pathogen might express antigens with epitopes that are structurally similar to epitopes of autoantigens, resulting in a mechanism of molecular mimicry. this is the case for helicobacter pylori-associa ... | 2004 | 15242679 |
| use of a novel enzyme immunoassay based on detection of circulating antigen in serum for diagnosis of helicobacter pylori infection. | recently, noninvasive diagnostic tests for helicobacter pylori infection have gained in significance. we have developed a sensitive and specific noninvasive immunoassay based on the detection of an h. pylori circulating antigen (hpca) in sera from h. pylori-infected individuals. monospecific antibody and western blot analyses were used to demonstrate the presence of the target antigen in h. pylori cell lysate and serum samples. a novel enzyme-linked immunosorbent assay (elisa) was developed for ... | 2004 | 15242956 |
| inhibition of helicobacter pylori adhesion to human gastric adenocarcinoma epithelial cells by acidic polysaccharides from artemisia capillaris and panax ginseng. | helicobacter pylori specifically adheres to host cells, mainly based on carbohydrate-mediated cell-cell interactions. previously, we investigated the anti-adhesive effect of polysaccharide fractions from artemisia capillaris and panax ginseng, using hemagglutination and enzyme-linked glycosorbent assays. in the present study, each active polysaccharide fraction was further purified, resulting in a single peak (fraction f2) using gel filtration fplc, in which no protein content was detectable. us ... | 2004 | 15254854 |
| elevated concentrations of alpha-defensins in gastric juice of patients with helicobacter pylori infection. | defensins (alpha- and beta-defensins) are endogenous antimicrobial peptides. little is known about alpha-defensins during helicobacter pylori infection. | 2004 | 15447750 |
| a confirmatory report for the close interaction of helicobacter pylori with gastric epithelial muc5ac expression. | helicobacter pylori (h. pylori) infection is associated with the development of gastritis and peptic ulcer and is presumed to be a risk factor for low-grade b-cell lymphoma and gastric cancer. h. pylori also causes critical alterations in gastric mucin structure. our aim was to determine the effect of h. pylori on muc1, muc2, and muc5ac expression. | 2004 | 15220684 |
| increased oxidative dna damage, inducible nitric oxide synthase, nuclear factor kappab expression and enhanced antiapoptosis-related proteins in helicobacter pylori-infected non-cardiac gastric adenocarcinoma. | several epidemiological studies have demonstrated a close association between helicobacter pylori (h pylori) infection and non-cardiac carcinoma of the stomach. h pylori infection induces active inflammation with neutrophilic infiltrations as well as production of oxygen free radicals that can cause dna damage. the dna damage induced by oxygen free radicals could have very harmful consequences, leading to gene modifications that are potentially mutagenic and/or carcinogenic. the aims of the pres ... | 2004 | 15259072 |
| specific immunoglobulin a antibodies in maternal milk and delayed helicobacter pylori colonization in gambian infants. | immunoglobulin a (iga) in maternal milk may protect gambian infants from early helicobacter pylori colonization. this study sought evidence that this protection could be due to specific iga antibodies. | 2004 | 15486839 |
| nod1 responds to peptidoglycan delivered by the helicobacter pylori cag pathogenicity island. | epithelial cells can respond to conserved bacterial products that are internalized after either bacterial invasion or liposome treatment of cells. we report here that the noninvasive gram-negative pathogen helicobacter pylori was recognized by epithelial cells via nod1, an intracellular pathogen-recognition molecule with specificity for gram-negative peptidoglycan. nod1 detection of h. pylori depended on the delivery of peptidoglycan to host cells by a bacterial type iv secretion system, encoded ... | 2004 | 15489856 |
| chemotactic response of helicobacter pylori to human plasma and bile. | to clarify further the role of chemotaxis in helicobacter pylori colonization, the in vitro bacterium response to human plasma and bile (secretions containing chemoeffector compounds that are present in the gastric mucus layer) was examined. human plasma, after dilution to 1 % (v/v) with buffer, was found to be a chemoattractant for the motile bacillus. human gall-bladder bile, after dilution to 2 % (v/v) with buffer, was found to be a chemorepellent, but did not cause the motility of the bacill ... | 2004 | 15272070 |
| helicobacter pylori in children and adolescents. | there is now considerable evidence that suggests that the h. pylori organism isa human pathogen. the strong association between h. pylori and gastroduodenal disease is well documented. a number of hypotheses have been suggested for the pathogenic mechanisms of h. pylori-induced gastroduodenal disease, including the presence of bacterial virulence factors, the production of inflammatory mediators, disregulation of acid secretion, and the host immune response. at the present time, treatment with a ... | 2004 | 15272256 |
| gastric epithelial cell cxc chemokine secretion following helicobacter pylori infection in vitro. | helicobacter pylori infection of the stomach is commonly associated with infiltration of neutrophils. gastric epithelial cells are recognized as central mediators of tissue responses to this organism. the aim of the present study was to ascertain patterns of production of three neutrophil chemoattractant chemokines following infection of gastric epithelial cells with h. pylori in vitro. | 2004 | 15304113 |
| sensitivity of helicobacter pylori to an innate defence mechanism, the lactoperoxidase system, in buffer and in human whole saliva. | helicobacter pylori has frequently been isolated from human dental plaque, and oral spread via saliva is thought to be one of its principal modes of transmission. among other innate defence systems human saliva contains peroxidase enzymes and lysozyme. the sensitivity of h. pylori to physiological concentrations of lactoperoxidase and its salivary substrate thiocyanate, and different amounts of hydrogen peroxide (h(2)o(2)) was investigated in buffer and in human whole saliva. the effect of lysoz ... | 2004 | 15314191 |
| differential expression of bax and bcl-2 in gastric mucosa. | 2004 | 15326840 | |
| downregulation of cxcr1 and cxcr2 expression on human neutrophils by helicobacter pylori: a new pathomechanism in h. pylori infection? | in helicobacter pylori gastritis, neutrophil activation and migration, which play central roles in the pathogenesis of the disease, are regulated by the neutrophil attractant chemokines interleukin 8 (il-8) and groalpha, whose secretion is induced by h. pylori. however, the modulation of the corresponding chemokine receptors cxcr1 and cxcr2 on human neutrophils under the influence of h. pylori has not been investigated. incubation of neutrophils with cag(+) and cag deletion h. pylori strains res ... | 2004 | 15557597 |
| influence of gastritis on cyclooxygenase-2 expression before and after eradication of helicobacter pylori infection. | helicobacter pylori infection causes chronic gastritis and induces cyclooxygenase (cox)-2 expression. the relationship between gastritis and cox-2 expression is not well understood, especially long after the organism has been eradicated. we designed a study to elucidate this relationship. | 2004 | 15371919 |
| cyp2c19 polymorphism is a major predictor of treatment failure in white patients by use of lansoprazole-based quadruple therapy for eradication of helicobacter pylori. | proton pump inhibitors, metabolized by the polymorphic enzyme cytochrome p450 (cyp) 2c19, are essential drugs for helicobacter pylori eradication. it was reported that patients with cyp2c19 wild type in asia had lower eradication rates. this study tests the hypothesis that cyp2c19 wild type ( wt/wt ) in white patients is also associated with a higher probability of treatment failure. | 2004 | 15371981 |
| essential domain of receptor tyrosine phosphatase beta (rptpbeta) for interaction with helicobacter pylori vacuolating cytotoxin. | helicobacter pylori produces a potent exotoxin, vaca, which causes progressive vacuolation as well as gastric injury. although vaca was able to interact with two receptor-like protein tyrosine phosphatases, rptpbeta and rptpalpha, rptpbeta was found to be responsible for gastric damage caused by vaca. to define the region of rptpbeta involved in vaca binding, we made mutants of human cdna rptpbeta-b, a short receptor form of rptpbeta. immunoprecipitation experiments to assess vaca binding to rpt ... | 2004 | 15383529 |
| nutrients released by gastric epithelial cells enhance helicobacter pylori growth. | helicobacter pylori survives and proliferates in the human gastric mucosa. in this niche, h. pylori adheres to the gastric epithelial cells near the tight junctions. in vitro, h. pylori proliferated well in tissue-culture medium near gastric epithelial cells. however, in the absence of epithelial cells, growth of h. pylori could only be established in tissue-culture medium when, prior to the experiment, it was preincubated near gastric epithelial cells. therefore, we aimed to determine whether d ... | 2004 | 15610074 |
| protective mechanism of epigallocatechin-3-gallate against helicobacter pylori-induced gastric epithelial cytotoxicity via the blockage of tlr-4 signaling. | helicobacter pylori infection leads to gastric mucosal damage by several mechanisms including the direct effect of virulence factors produced by h. pylori, propagation of inflammation, oxidative stress, dna damage, and induction of apoptosis. (-)-epigallocatechin-3-gallate (egcg), one of the green tea catechins, is known to suppress h. pylori-induced gastritis through its antioxidative and antibacterial actions. in this study, we evaluated the protective mechanism of egcg against h. pylori-induc ... | 2004 | 15610077 |
| solving the riddle of codon usage preferences: a test for translational selection. | translational selection is responsible for the unequal usage of synonymous codons in protein coding genes in a wide variety of organisms. it is one of the most subtle and pervasive forces of molecular evolution, yet, establishing the underlying causes for its idiosyncratic behaviour across living kingdoms has proven elusive to researchers over the past 20 years. in this study, a statistical model for measuring translational selection in any given genome is developed, and the test is applied to 1 ... | 2004 | 15448185 |
| [genetic susceptibility and risk of gastric cancer in a human population of cauca, colombia]. | gastric cancer (gc) is the main cause of mortality by cancer in colombia. glutathione s-transferase (gst) enzymes are involved in the detoxification of many environmental carcinogens. the homozygous deletions of glutathione s-transferase m1 (gstm1-0) and glutathione s-transferase t1 (gstt1-0) have been associated with several types of cancer. the risk to develop gc has been associated with environmental factors and helicobacter pylori infection. the tumor necrosis factor (tnf-alpha) and its leve ... | 2004 | 15495595 |
| detection of cytomegalovirus, helicobacter pylori and chlamydia pneumoniae dna in carotid atherosclerotic plaques by the polymerase chain reaction. | cytomegalovirus (cmv), helicobacter pylori (h. pylori) and chlamydia pneumoniae (c. pneumoniae) have been associated with human atherosclerosis. the reported rates of detection within atherosclerotic lesions by pcr vary widely for all of these pathogens. we investigated their presence in carotid atherosclerotic plaques. | 2004 | 15636450 |
| review article: relationship between the metabolism and efficacy of proton pump inhibitors--focus on rabeprazole. | proton pump inhibitors are now considered the mainstay of treatment for acid-related disease. although all proton pump inhibitors are highly effective, the antisecretory effects of different drugs in this class are not completely consistent across patients. one reason for this is the acid-suppressing effect of helicobacter pylori infection, which may augment the actions of proton pump inhibitors. a second important reason for interpatient variability of the effects of proton pump inhibitors on a ... | 2004 | 15496214 |
| functional adaptation of baba, the h. pylori abo blood group antigen binding adhesin. | adherence by helicobacter pylori increases the risk of gastric disease. here, we report that more than 95% of strains that bind fucosylated blood group antigen bind a, b, and o antigens (generalists), whereas 60% of adherent south american amerindian strains bind blood group o antigens best (specialists). this specialization coincides with the unique predominance of blood group o in these amerindians. strains differed about 1500-fold in binding affinities, and diversifying selection was evident ... | 2004 | 15273394 |
| diagnosis of helicobacter pylori infection in dyspeptic patients by stool antigen detection usefulness of a new monoclonal enzyme immunoassay test. | helicobacter pylori antigens can be measured in human stools with an enzyme immunoassay, which may prove to be a valuable non-invasive diagnostic tool. aim. to evaluate the usefulness of a new monoclonal enzyme immunoassay for detecting h. pylori antigens in dyspeptic patients' faeces (femtolab h. pylori, connex, martinsried, germany). | 2004 | 15285523 |
| human polymeric iga is superior to igg and single-chain fv of the same monoclonal specificity to inhibit urease activity associated with helicobacter pylori. | helicobacter-induced gastritis is considered nowadays an epidemic, the prevalence of which is one of the highest world-wide (70%), with as much as 40% of the population in industrialized countries. helicobacter pylori (h. pylori) antigens (ag) capable to elicit a protective immune response in animal models have been identified, but these antigens have not been shown to be strongly immunogenic when administered to humans. due to their stability in the gastric environment and avidity, passive admi ... | 2004 | 15302163 |
| [probiotics: from metchnikoff to the current preventive and therapeutic possibilities]. | about a century ago, metchnikoff first hypothesised that some intestinal bacteria "produce compounds useful against a premature ageing". since then, studies progressed over last century, leading to a remarkable improvement of the knowledge about the role of intestinal micro-organisms. nowadays a number of different micro-organisms satisfying certain requisites are named probiotics and are produced on a large scale. at present, a rational use of probiotics with preventive and therapeutic purposes ... | 2004 | 15529809 |
| regression of gastric malt-lymphoma under specific therapy may be predict by endoscopic ultrasound. | mucosa-associated lymphoid tissue (malt) lymphomas represent a relatively new described class of rare lymphomas, characterized by an indolent course and favourable outcome with specific therapy. gastric malt lymphomas are associated with chronic helicobacter pylori (hp) infection. we report the case of a 67 year old man admitted for an 8-month history of epigastric pain, anorexia and progressive weight loss. he was diagnosed with low-grade primary gastric malt lymphoma by endoscopy, histopatholo ... | 2004 | 15229778 |
| novel 180- and 480-base-pair insertions in african and african-american strains of helicobacter pylori. | helicobacter pylori is a genetically diverse bacterial species that chronically infects human stomachs and sometimes causes severe gastroduodenal disease. studies of polymorphic dna sequences can suggest geographic origins of individual strains. here, we describe a 180-bp insertion (ins180), which is just after the translation stop of a gene of unknown function, near the promoter of jhp0152-jhp0151 two-component signal transduction genes in strain j99, and absent from this site in strain 26695. ... | 2004 | 15583296 |
| oral broccoli sprouts for the treatment of helicobacter pylori infection: a preliminary report. | we sought to determine whether orally consumed broccoli sprouts could eradicate helicobacter pylori infection in infected human volunteers. helicobacter pylori-positive patients were identified by stool antigen testing or gastric biopsies. patients consumed broccoli sprouts (14, 28, or 56 g) twice daily for 7 days. we performed stool antigen testing immediately following the completion of treatment (day 8) and at day 35. urea breath testing was performed on those patients who remained negative a ... | 2004 | 15387326 |
| the diurnal profile of gastric pepsin activity is reduced with helicobacter pylori infection. | both helicobacter pylori and pepsin are proven mucosal damaging agents and implicated in the aetiology of peptic ulcer disease. historically studies of pepsin over time have proved methodologically difficult, and as a result little work has been done on the effect of h. pylori on luminal pepsin secretion. our objectives were to determine pepsin activity over 24 hr in normal human subjects and to examine luminal pepsin activity in relation to h. pylori infection. twenty-seven healthy volunteers h ... | 2004 | 15387329 |
| experimental helicobacter pylori infection in humans: a multifaceted challenge. | 2004 | 15306572 | |
| challenge model for helicobacter pylori infection in human volunteers. | a reliable challenge model is needed to evaluate helicobacter pylori vaccine candidates. | 2004 | 15306577 |
| extracellular signal-regulated protein kinase mediates interleukin 17 (il-17)-induced il-8 secretion in helicobacter pylori-infected human gastric epithelial cells. | helicobacter pylori-induced mucosal inflammation results in high production of interleukin 17 (il-17), a potent inducer of il-8 in gastric epithelial cells. the aim of this study was to investigate signaling pathways by which il-17 regulates il-8 production in human gastric epithelial cells. activation of mitogen-activated protein (map) kinases in both il-17-stimulated mkn28 cells and epithelial cells isolated from h. pylori-colonized gastric mucosa was assessed by western blotting. in il-17-sti ... | 2004 | 15321994 |
| one patient, two lymphomas. simultaneous primary gastric marginal zone lymphoma and primary duodenal follicular lymphoma. | we report the case of a 73-year-old man who presented with a 2- to 3-month history of epigastric discomfort and guaiac-positive stool. an upper gastrointestinal endoscopy revealed a diffuse erythematous nodular mucosa and submucosal thickening in the stomach. diffuse mucosal nodularity was also found in the second portion of the duodenum. a complete workup with histologic, immunohistochemical, and molecular studies revealed 2 distinct, apparently unrelated lymphomas, namely, a gastric marginal z ... | 2004 | 15335250 |
| [pathogenesis and treatment of refractory gastroesophageal reflux disease in japanese patients]. | prevalence of refractory gastroesophageal reflux disease (gerd) defined as a patient who have persistent gerd symptoms during treatment with proton pump inhibitor (ppi) is rare in japanese patinets. pathogenesis of refractory gerd is associated with several factors including dysfunction of esophageal motility, presence of severe hiatal hernia, complication such as stricture and short esophagus, extensive metabolizer of cyp2c19 genotype, nocturnal gastric acid breakthrough, absence of h. pylori i ... | 2004 | 15344542 |
| effect of h. pylori on the expression of trail, fasl and their receptor subtypes in human gastric epithelial cells and their role in apoptosis. | in the human stomach expression of tnf-related apoptosis inducing ligand (trail) and its receptors and the modulatory role of helicobacter pylori are not well described. therefore, we investigated the effect of h. pylori on the expression of trail, fasl and their receptors (trail-r1-r4, fas) in gastric epithelial cells and examined their role in apoptosis. | 2004 | 15361075 |
| geranylgeranylacetone attenuates suppression by helicobacter pylori extract of human umbilical vein epithelial cell growth. | helicobacter pylori infection delays gastric ulcer healing. angiogenesis is important for the healing of gastric ulcers. therefore, the effects of h. pylori water extract and a novel antiulcer drug, geranylgeranylacetone, on the viability of human umbilical vein epithelial cells (huvecs) were investigated. | 2004 | 15362801 |
| helicobacter pylori infection in pediatric population: epidemiology, pathophysiology, and therapy. | helicobacter pylori, one of the most commonly encountered human pathogens, has been recognized in more than half of adults and children with chronic nonspecific gastritis, and nearly all children with peptic ulcer disease demonstrate antral infection with h. pylori. a high prevalence and early colonization of h. pylori infection in childhood was described in developing countries in contrast to developed countries. the current diagnostic methods include histology, microbiological culture, urease ... | 2004 | 15371120 |
| pathogen burden, inflammation, proliferation and apoptosis in human in-stent restenosis. tissue characteristics compared to primary atherosclerosis. | pathogenic events leading to in-stent restenosis (isr) are still incompletely understood. among others, inflammation, immune reactions, deregulated cell death and growth have been suggested. therefore, atherectomy probes from 21 patients with symptomatic isr were analyzed by immunohistochemistry for pathogen burden and compared to primary target lesions from 20 stable angina patients. while cytomegalovirus, herpes simplex virus, epstein-barr virus and helicobacter pylori were not found in isr, a ... | 2004 | 15528935 |
| spermine oxidation induced by helicobacter pylori results in apoptosis and dna damage: implications for gastric carcinogenesis. | oxidative stress is linked to carcinogenesis due to its ability to damage dna. the human gastric pathogen helicobacter pylori exerts much of its pathogenicity by inducing apoptosis and dna damage in host gastric epithelial cells. polyamines are abundant in epithelial cells, and when oxidized by the inducible spermine oxidase smo(paoh1) h(2)o(2) is generated. here, we report that h. pylori up-regulates mrna expression, promoter activity, and enzyme activity of smo(paoh1) in human gastric epitheli ... | 2004 | 15574757 |
| the adherent gastric mucous layer is composed of alternating layers of muc5ac and muc6 mucin proteins. | mucin-type glycoproteins are the major structural proteins in gastric mucus. stomach mucin proteins include muc5ac, synthesized by surface foveolar or pit cells, and muc6, synthesized by neck and gland cells. the aim of this study was to determine the spatial distribution of these mucin proteins within the extracellular mucous coat. double-labeling immunoflourescence/confocal microscopy was used in histologically normal surgical resection specimens. intralumenal mucin within antral glands consis ... | 2004 | 15573912 |
| helicobacter-induced intestinal metaplasia in the stomach correlates with elk-1 and serum response factor induction of villin. | chronic helicobacter pylori infection results in serious sequelae, including atrophy, intestinal metaplasia, and gastric cancer. intestinal metaplasia in the stomach is defined by the presence of intestine-like cells expressing enterocyte-specific markers, such as villin. in this study, we demonstrate that villin is expressed in intestine-like cells that develop after chronic infection with h. pylori in both human stomach and in a mouse model. transfection studies were used to identify specific ... | 2004 | 15576363 |
| gastric malt lymphomas are divided into three groups based on responsiveness to helicobacter pylori eradication and detection of api2-malt1 fusion. | gastric malt lymphoma shows unique features including regression by helicobacter pylori eradication and api2-malt1 fusion. we performed a molecular and clinicopathologic study for 115 cases. all eradication-responsive cases were devoid of api2-malt1 fusion. all tumors positive for the fusion and all negative for h. pylori infection were nonresponsive to the eradication. consequently, gastric malt lymphomas were divided into three groups: eradication-responsive and fusion-negative (group a, n = 7 ... | 2004 | 15577674 |
| the roles of cytokines, inflammation and immunity in vascular diseases. | recent findings regarding the roles of cytokines, inflammation and immunity during the development of atherosclerosis were reviewed. especially, the relationships among pro-inflammatory cytokines such as interleukin (il)-1, il-18 and osteopontin, and anti-inflammatory cytokines such as il-1 receptor antagonist, il-10 and il-18 binding protein to inflammation and atherosclerosis were investigated and are described in detail. in addition, helicobacter pylori and c pneumoniae infections to inflamma ... | 2004 | 15644584 |
| upregulated akt signaling adjacent to gastric cancers: implications for screening and chemoprevention. | most gastric adenocarcinomas arise as a longterm complication of helicobacter pylori infection of the stomach, but the high prevalence of this infection limits the cost-effectiveness of antibiotic eradication as a cancer prevention strategy. here we have used phosphorylation-specific antibodies against the akt kinase consensus sequence to detect downstream substrates of this oncogenic signaling pathway in normal and malignant gastric tissues. in vitro studies confirm that phosphorylation of akt ... | 2004 | 15922857 |
| induction of peripheral lymph node addressin in human gastric mucosa infected by helicobacter pylori. | helicobacter pylori infects over half the world's population and is a leading cause of peptic ulcer and gastric cancer. h. pylori infection results in chronic inflammation of the gastric mucosa, and progression of chronic inflammation leads to glandular atrophy and intestinal metaplasia. however, how this chronic inflammation is induced or maintained is not well known. here, we show that chronic inflammation caused by h. pylori infection is highly correlated with de novo synthesis of peripheral ... | 2004 | 15591109 |
| role of the receptor-mediated apoptosis in helicobacter pylori in gastric epithelial cells. | two major pathways leading to apoptosis have been described. it has been shown that helicobacter pylori-mediated apoptosis is mainly effected through the mitochondrial pathway (type ii). the role of the type i pathway, including the death receptors, has been discussed controversially. therefore, we investigated the role of fas ligand (fasl) and trail in h. pylori-mediated apoptosis by overexpressing antiapoptotic proteins in the human gastric epithelial cell line ags. | 2004 | 15557763 |
| gene expression in gastric biopsies from patients infected with helicobacter pylori. | helicobacter pylori infection has protean effects on gene expression in the host gastric mucosa, which have been investigated by gene chip analysis in vitro. in this study the effects of h. pylori infection on host gene expression in the gastric antral mucosa in patients were examined. | 2004 | 15742995 |
| the usefulness of stable isotopes in nutrition and human health: the application of mass spectrometry and 13c-breath tests to detect helicobacter pylori infection. | the interest in helicobacter pylori has escalated in recent years. h. pylori may produce a chronic gastric infection which is usually life-long and many epidemiological studies have shown that this is the most common bacterial infection throughout the world involving 50% of the world population. thus, it is clear that the diagnosis of h. pylori infection represents at least a key step in the management of many of the patients referred to the gastroenterologist. additionally, due to the wide rang ... | 2004 | 16119720 |
| the selective cyclooxygenase-2 inhibitor nimesulide prevents helicobacter pylori-associated gastric cancer development in a mouse model. | helicobacter pylori infection can lead to gastric cancer, and cyclooxygenase-2 (cox-2) is overexpressed in the stomach during h. pylori infection. therefore, we investigated whether nonsteroidal anti-inflammatory drugs might protect against this form of cancer. specifically, we examined the chemopreventive effect of the cox-2 inhibitor nimesulide on h. pylori-associated gastric carcinogenesis in mice. | 2004 | 15585646 |
| helicobacter pylori causes gastric cancer by hijacking cell growth signaling. | extract: infection with certain strains (carrying the caga gene) of the bacterium helicobacter pylori (h. pylori) is associated with the development of gastric carcinoma. upon introduction into the gastric epithelial cells and its subsequent modification by phosphorylation (adding a phosphate group) at the tyrosine amino acid residue, caga binds specifically to a cellular oncoprotein (shp-2) and causes the oncoprotein's phosphatase activity (ridding is a phosphate group from a molecule) to misbe ... | 2004 | 20704951 |
| the association of helicobacter pylori infection with coronary artery disease: fact or fiction? | helicobacter pylori (h. pylori) infection is an important cause of peptic ulcer disease and other gastrointestinal disorders. since its discovery, a number of extra-gastrointestinal diseases have been reported to be associated with h. pylori infection. recently, several epidemiological and clinical studies suggested that h. pylori infection has been associated with an increased risk of developing coronary artery disease (cad). evidence from animal studies showed that h. pylori plays an important ... | 2004 | 19861836 |
| herpes simplex virus 1 induced lox-1 expression in an endothelial cell line, ecv 304. | infections, such as by chlamydophilia pneumoniae, cytomegalovirus, herpes simplex virus, and helicobacter pylori, have been shown to be involved in atherogenesis. herpes simplex virus i (hsv-1) could infect vascular endothelial cells, and it has been shown that, when endothelial cells were activated with oxidized ldl (oxldl), a number of cellular events are occurred, leading to endothelial cell dysfunction. since lox-1 is a major receptor for oxldl on endothelial cells and its expression was inc ... | 2004 | 15279708 |
| inhibition of primary human t cell proliferation by helicobacter pylori vacuolating toxin (vaca) is independent of vaca effects on il-2 secretion. | recent evidence indicates that the secreted helicobacter pylori vacuolating toxin (vaca) inhibits the activation of t cells. vaca blocks il-2 secretion in transformed t cell lines by suppressing the activation of nuclear factor of activated t cells (nfat). in this study, we investigated the effects of vaca on primary human cd4(+) t cells. vaca inhibited the proliferation of primary human t cells activated through the t cell receptor (tcr) and cd28. vaca-treated jurkat t cells secreted markedly d ... | 2004 | 15128946 |
| transcription profile of helicobacter pylori in the human stomach reflects its physiology in vivo. | little is known about levels of expression of helicobacter pylori genes in the human host. we therefore developed a quantitative real-time reverse-transcriptase polymerase chain reaction (qrt-pcr) assay to measure transcript profiles of h. pylori in the human stomach. | 2004 | 15295700 |
| development of two pcr-based techniques for detecting helical and coccoid forms of helicobacter pylori. | the primary mode of transmission of helicobacter pylori, a human pathogen carried by more than half the population worldwide, is still unresolved. some epidemiological data suggest water as a possible transmission route. h. pylori in the environment transforms into a nonculturable, coccoid form, which frequently results in the failure to detect this bacterium in environmental samples by conventional culture techniques. to overcome limitations associated with culturing, molecular approaches based ... | 2004 | 15297506 |
| analysis of geospecific markers for helicobacter pylori variants in patients from japan and nigeria by triple-locus nucleotide sequence typing. | human migrations and geographical separation over long periods may have resulted in ecologically distinct populations of helicobacter pylori infecting individuals in different continents. this study used nucleotide sequence analysis with the aim of defining population-specific genomic motifs in isolates from east asian and african dyspeptic patients. sequences of internal fragments (542-627 bp) of three housekeeping genes (urei, ahpc and atpa) were analysed for 85 isolates from individuals in ja ... | 2004 | 14702408 |
| infiltration of cd8+ t cells containing rantes/ccl5+ cytoplasmic granules in actively inflammatory lesions of human chronic gastritis. | chronic gastritis is frequently associated with infection of helicobacter pylori and characterized by tissue infiltration of neutrophils, lymphocytes, and plasma cells. to address the mechanism of lymphocyte infiltration in chronic gastritis, we examined the expression of chemokines and their receptors using frozen sections of chronic gastritis, obtained from 23 patients who underwent gastrectomy for gastric cancer. by immunohistochemistry, lymphocytes in inflamed gastric mucosa expressed ccr5 a ... | 2004 | 14704722 |
| oxidative-stress-related proteome changes in helicobacter pylori-infected human gastric mucosa. | helicobacter pylori infection leads to gastroduodenal inflammation, peptic ulceration and gastric carcinoma. proteomic analysis of the human gastric mucosa from the patients with erosive gastritis, peptic ulcer or gastric cancer, which were either infected or not with h. pylori, was used to determine the differentially expressed proteins by h. pylori in the human gastric mucosa in order to investigate the pathogenic mechanism of h. pylori -induced gastric diseases. prior to the experiment, the e ... | 2004 | 14711373 |
| helicobacter pylori and gastroesophageal reflux disease. | since the rediscovery of helicobacter pylori two decades ago, it has become increasingly clear that the true relationships between this organism and diseases of the upper gastrointestinal tract are highly complex. h. pylori colonization is a strong risk factor for peptic ulceration and distal gastric cancer; however, gastritis has no adverse consequences for most hosts, and the prevalence of h. pylori is inversely related to gastroesophageal reflux disease (gerd) and its sequelae, which include ... | 2004 | 14723839 |