Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
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| palladium(ii) complexes of os donor n-(di(butyl/phenyl)carbamothioyl)benzamide and their antiamoebic activity. | two promising palladium(ii) compounds of general formula, cis-[pd(l-o,s)2] [where hl-o,s = n-(di(butyl/phenyl)carbamothioyl)benzamide] as metal based antiamoebic drug candidates, have been synthesized. both complexes are characterized in the solid state by ft-ir spectroscopy, tga and single crystal x-ray study, as well as in solution by other spectroscopic techniques such as (1)h and (13)c nmr, and uv-visible. all these studies confirm the coordination of ligands through oxygen and sulphur atoms ... | 2015 | 26005916 |
| parasitism in children aged three years and under: relationship between infection and growth in rural coastal kenya. | parasitic infections, which are among the most common infections worldwide, disproportionately affect children; however, little is known about the impact of parasitic disease on growth in very early childhood. our objective was to document the prevalence of parasitic infections and examine their association with growth during the first three years of life among children in coastal kenya. | 2015 | 25996157 |
| a rare case of multilocus brain abscess due to entamoeba histolytica infection in a child. | 2015 | 25987125 | |
| entamoeba mitosomes play an important role in encystation by association with cholesteryl sulfate synthesis. | hydrogenosomes and mitosomes are mitochondrion-related organelles (mros) that have highly reduced and divergent functions in anaerobic/microaerophilic eukaryotes. entamoeba histolytica, a microaerophilic, parasitic amoebozoan species, which causes intestinal and extraintestinal amoebiasis in humans, possesses mitosomes, the existence and biological functions of which have been a longstanding enigma in the evolution of mitochondria. we previously demonstrated that sulfate activation, which is not ... | 2015 | 25986376 |
| amoeboma-lest we forget!!! | amoebiasis is an infection with the parasitic intestinal protozoan entamoeba histolytica (e. histolytica). most infections are usually asymptomatic, but the disease spectrum can range from dysentery to extraintestinal infections, including liver abscesses. the management of a 68-year-old male with amoeboma, a rare manifestation of intestinal amoebiasis, is described along with a review of literature. | 2015 | 25972693 |
| [coecum amoebiasis can be a rare manifestation of entamoeba histolytica infection]. | a 58-year-old danish male was admitted to hospital with acute abdominal pain. laparoscopy showed a normal appendix. however, a tumour in the ascending colon was found and hemicolectomy was performed. pathological examination showed deep ulcerations with undermined edges and massive inflammation and necrosis. periodic acid schiff (pas)-positive amoebae trophozoites were identified, and the patient was diagnosed with and treated for amoebiasis. the patient had not been abroad for 14 years but he w ... | 2015 | 25967092 |
| iron status of schoolchildren (6-15 years) and associated factors in rural nigeria. | schoolchildren are vulnerable to anaemia because of their higher iron need to meet the demands of puberty and adolescence. | 2015 | 25952679 |
| structure of an adp-ribosylation factor, arf1, from entamoeba histolytica bound to mg(2+)-gdp. | entamoeba histolytica is the etiological agent of amebiasis, a diarrheal disease which causes amoebic liver abscesses and amoebic colitis. approximately 50 million people are infected worldwide with e. histolytica. with only 10% of infected people developing symptomatic amebiasis, there are still an estimated 100,000 deaths each year. because of the emergence of resistant strains of the parasite, it is necessary to find a treatment which would be a proper response to this challenge. adp-ribosyla ... | 2015 | 25945714 |
| structures of a histidine triad family protein from entamoeba histolytica bound to sulfate, amp and gmp. | three structures of the histidine triad family protein from entamoeba histolytica, the causative agent of amoebic dysentery, were solved at high resolution within the seattle structural genomics center for infectious disease (ssgcid). the structures have sulfate (pdb entry 3oj7), amp (pdb entry 3omf) or gmp (pdb entry 3oxk) bound in the active site, with sulfate occupying the same space as the α-phosphate of the two nucleotides. the c(α) backbones of the three structures are nearly superimposabl ... | 2015 | 25945711 |
| characterization of ph-induced transitions of entamoeba histolytica d-phosphoglycerate dehydrogenase. | entamoeba histolytica d-phosphoglycerate dehydrogenase (ehpgdh) exists as a functionally active homodimer at ph 7. our earlier studies have shown that ionic interactions are essentially required for the oligomeric status and activity of the protein. present study focuses on ph associated structural modulations of ehpgdh. far-uv cd spectra showed loss in the secondary structure of the protein as a function of low ph, however, the protein was not completely unfolded even at ph 2. energy minimized ... | 2015 | 25944370 |
| amino acid residues leu135 and tyr236 are required for rna binding activity of cfim25 in entamoeba histolytica. | pre-mrna 3' end processing in the nucleus is essential for mrna stability, efficient nuclear transport, and translation in eukaryotic cells. in human, the cleavage/polyadenylation machinery contains the 25 kda subunit of the cleavage factor im (cfim25), which specifically recognizes two ugua elements and regulates the assembly of polyadenylation factors, poly(a) site selection and polyadenylation. in entamoeba histolytica, the protozoan parasite responsible for human amoebiasis, ehcfim25 has bee ... | 2015 | 25941172 |
| detection and molecular characterisation of giardia duodenalis, cryptosporidium spp. and entamoeba spp. among patients with gastrointestinal symptoms in gambo hospital, oromia region, southern ethiopia. | to assess the prevalence and genetic diversity of the enteric protozoa species g. duodenalis, cryptosporidium spp. and entamoeba histolytica in individuals with gastrointestinal symptoms compatible with infections by these pathogens seeking medical attention in a rural area in southern ethiopia. | 2015 | 25939247 |
| atg8 is involved in endosomal and phagosomal acidification in the parasitic protist entamoeba histolytica. | autophagy is one of two major bulk protein degradation systems and is conserved throughout eukaryotes. the protozoan entamoeba histolytica, which is a human intestinal parasite, possesses a restricted set of autophagy-related (atg) proteins compared with other eukaryotes and thus represents a suitable model organism for studying the minimal essential components and ancestral functions of autophagy. e. histolytica possesses two conjugation systems: atg8 and atg5/12, although a gene encoding atg12 ... | 2015 | 25923949 |
| [amoebic liver abscess in a patient initially diagnosed with pneumonia: case report and discussion of relevant literature]. | in one-third of the patients with amoebiasis, amoebic liver abscess (ala) may occur after the penetration of amoebic trophozoites through the intestinal wall. ala is seen mostly among men aged 20-45 years with a serious clinical outcome, with fever and abdominal pain on the right upper quadrant. most patients have no recent history of amoebic colitis; indeed, they have neither gastrointestinal complaints nor entamoeba histolytica (e. histolytica) cysts/trophozoites in their stools. therefore, ul ... | 2015 | 25917589 |
| identification of entamoeba histolytica by molecular method in surface water of rasht city, iran. | this study was conducted with the aim of determining surface water contamination with cysts of entamoeba histolytica using pcr in rasht city, northern iran. | 2015 | 25905058 |
| entamoeba histolytica: protein arginine transferase 1a methylates arginine residues and potentially modify the h4 histone. | in eukaryotes, histone arginine methylation associates with both active and repressed chromatin states depending on the residues involved and the status of methylation. even when the amino-terminus of entamoeba histolytica histones diverge from metazoan sequences, these regions contain arginine residues that are potential targets for methylation. however, histone arginine methylation as well as the activity of arginine methyltransferases (prmts) has not been studied in this parasite. the aim of ... | 2015 | 25889855 |
| entamoeba histolytica rhomboid protease 1 has a role in migration and motility as validated by two independent genetic approaches. | rhomboid proteins represent a recently discovered family of intramembrane proteases present in a broad range of organisms and with increasing links to human diseases. the enteric parasite entamoeba histolytica has evolved multiple mechanisms to adapt to the human host environment and establish infection. our recent studies identified ehrom1 as a functional e. histolytica rhomboid protease with roles in adhesion to and phagocytosis of host cells. since those studies were performed in a non-virule ... | 2015 | 25889553 |
| chemokine levels and parasite- and allergen-specific antibody responses in children and adults with severe or uncomplicated plasmodium falciparum malaria. | chemokine and antibody response profiles were investigated in children and adults with severe or uncomplicated plasmodium falciparum malaria; the aim was to reveal which profiles are associated with severe disease, as often seen in nonimmune children, or with mild and uncomplicated disease, as seen in semi-immune adults. blood samples were obtained from children under 5 years of age as well as adults with falciparum malaria. classification of malaria was performed according to parasite densities ... | 2015 | 25883801 |
| seroepidemiology of entamoeba histolytica infection in general population in rural durango, mexico. | the seroepidemiology of entamoeba histolytica in mexico has been scantily studied. the aim of the study was to determine the seroprevalence and correlates of e. histolytica antibodies in adults in rural areas in durango, mexico. | 2015 | 25883706 |
| assessments of immunomodulatory and inflammatory effects against induction of entamoeba histolytica (hm1 ims strain) crude extract antigen in complete freund's adjuvant induced rheumatoid arthritis female wistar rats. | today it is well known about mechanisms of cell communication, how the cells that mediate immune response and tissue injury accumulate in tissues but the aetiology of rheumatoid arthritis (ra) is still unknown. this study was to evaluate immunomodulatory effects of crude entamoeba histolytica (hm1 ims strain) antigen in complete freund's adjuvant female wistar rats by studying the alterations in humoral and cell mediated immune responses and also the inflammatory effects by evaluating the change ... | 2015 | 25881902 |
| antiprotozoal nitazoxanide derivatives: synthesis, bioassays and qsar study combined with docking for mechanistic insight. | in view of the serious health problems concerning infectious diseases in heavily populated areas, we followed the strategy of lead compound diversification to evaluate the near-by chemical space for new organic compounds. to this end, twenty derivatives of nitazoxanide (ntz) were synthesized and tested for activity against entamoeba histolytica parasites. to ensure drug-likeliness and activity relatedness of the new compounds, the synthetic work was assisted by a quantitative structure-activity ... | 2015 | 25872791 |
| roles and regulation of the mucus barrier in the gut. | the gastrointestinal tract is coated by a thick layer of mucus that forms the front line of innate host defense. mucus consists of high molecular weight glycoproteins called mucins that are synthesized and secreted by goblet cells and functions primarily to lubricate the epithelium and protect it from damage by noxious substances. recent studies have also suggested the involvement of goblet cells and mucins in complex immune functions such as antigen presentation and tolerance. under normal phys ... | 2015 | 25838985 |
| correction: crystal structure of calcium binding protein-5 from entamoeba histolytica and its involvement in initiation of phagocytosis of human erythrocytes. | 2015 | 25816121 | |
| pyrazolo[3,4-d]pyrimidines as novel inhibitors of o-acetyl-l-serine sulfhydrylase of entamoeba histolytica: an in silico study. | amoebiasis, a worldwide explosive epidemic, caused by the gastrointestinal anaerobic protozoan parasite entamoeba histolytica, infects the large intestine and, in advance stages, liver, kidney, brain and lung. metronidazole (mnz)-the first line medicament against amoebiasis-is potentially carcinogenic to humans and shows significant side-effects. pyrazolo[3,4-d]pyrimidine compounds have been reported to demonstrate antiamoebic activity. in silico molecular docking simulations on nine pyrazolo[3, ... | 2015 | 25799964 |
| homology modeling of mdr1 gene mdr1_enthi of e. histolytica & its molecular docking with anti-entamoeba histolytica agents. | to investigate the theoretical model of the three-dimensional structure of multidrug resistance protein 1(p-glycoprotein, expressed by mdr1 gene of e. histolytica) and its molecular docking with anti-amoebic agents. the theoretical model of multidrug resistance protein 1(p-glyoprotein, expressed by mdr1 gene of e. histolytica) was predicted by homology modeling on the structure of the 3g61. docking studies were performed to investigate the interaction of p-glycoprotein with antiamoebic gents. mr ... | 2015 | 25786505 |
| functional correlation of medicinal chemistry and entamoeba histolytica treatment: an emphasis on the past, present and future chemotherapy. | entamoeba being an internal parasite is the causative organism of many human and animal infections with lethal consequences. chemotherapy against entamoeba was well established by both synthetic and natural products. vigorous attainments were made by different researchers all over the world for discovering better chemical entities and the potent formulations. preexisting compounds were developed into non-cytotoxic and with an enhanced therapeutic compounds by usage of multiparametric optimizatio ... | 2015 | 25769970 |
| enteric pathogens of food sellers in rural gambia with incidental finding of myxobolus species (protozoa: myxozoa). | ongoing surveillance of enteric pathogens of public health significance among casual food sellers is undertaken in many resource-limited countries. we report the results of a survey in kiang west province, the gambia, and provide an exemplar methodology for such surveys in resource-limited laboratories. | 2015 | 25758855 |
| a rare case of multilocus brain abscess due to entamoeba histolytica infection in a child. | brain abscess due to entamoeba histolytica (e. histolytica) may pose a diagnostic problem or a therapeutic challenge, as evidenced by the paucity of papers reporting complete recovery after treatment. an 11-year-old girl presented with progressive drowsiness, diminished movements of the left upper limb, and swallowing problems. cranial mri showed multiple, contrast-dense masses with fluid content. she was started on meropenem. surgical drainage was performed. no bacterial or fungal growth was ob ... | 2015 | 25737180 |
| small gtpase rab21 mediates fibronectin induced actin reorganization in entamoeba histolytica: implications in pathogen invasion. | the protozoan parasite entamoeba histolytica causes a wide spectrum of intestinal infections. in severe cases, the trophozoites can breach the mucosal barrier, invade the intestinal epithelium and travel via the portal circulation to the liver, where they cause hepatic abscesses, which can prove fatal if left untreated. the host extra cellular matrix (ecm) plays a crucial role in amoebic invasion by triggering an array of cellular responses in the parasite, including induction of actin rich adhe ... | 2015 | 25730114 |
| new enzymatic pathways for the reduction of reactive oxygen species in entamoeba histolytica. | entamoeba histolytica, an intestinal parasite that is the causative agent of amoebiasis, is exposed to elevated amounts of highly toxic reactive oxygen and nitrogen species during tissue invasion. a flavodiiron protein and a rubrerythrin have been characterized in this human pathogen, although their physiological reductants have not been identified. | 2015 | 25725270 |
| a novel mitosomal β-barrel outer membrane protein in entamoeba. | entamoeba possesses a highly divergent mitochondrion-related organelle known as the mitosome. here, we report the discovery of a novel protein in entamoeba, which we name mitosomal β-barrel outer membrane protein of 30 kda (mbomp30). initially identified through in silico analysis, we experimentally confirmed that mbomp30 is indeed a β-barrel protein. circular dichroism analysis showed mbomp30 has a predominant β-sheet structure. localization to entamoeba histolytica mitosomes was observed throu ... | 2015 | 25711150 |
| comparison of the anaerocult a and the oil blocking methods for the in vitro cultivation of entamoeba histolytica. | entamoeba histolytica, the causative agent for human amoebiasis, is among the most deadly parasites, accounting for the second highest mortality rate among parasitic diseases. because this parasite dwells in low oxygen tension, for its cultivation, microaerophilic conditions are required to mimick the human gut environment. several methods developed for optimal growth environment are commercially available and some are conventionally modified in-house which include the anaerocult a and oil block ... | 2015 | 26712674 |
| immune evasion mechanisms of entamoeba histolytica: progression to disease. | entamoeba histolytica (eh) is a protozoan parasite that infects 10% of the world's population and results in 100,000 deaths/year from amebic dysentery and/or liver abscess. in most cases, this extracellular parasite colonizes the colon by high affinity binding to muc2 mucin without disease symptoms, whereas in some cases, eh triggers an aggressive inflammatory response upon invasion of the colonic mucosa. the specific host-parasite factors critical for disease pathogenesis are still not well cha ... | 2015 | 26696997 |
| first report of genitourinary amoebiasis in thailand. | genitourinary amoebiasis is a rare form of kidney and urinary tract infection caused by entamoeba histolytica. here, we describe the first case of unusual infection caused by e. histolytica in bueng kan province, northeast, thailand. a 63-year-old female patient was diagnosed as acute pyelonephritis. e. histotolytica trophozoites were accidentally found in urine collected from a catheter. | 2015 | 26695217 |
| scrotal abscess, a rare case of extra intestinal amoebiasis. | the majority of amoeba infection are asymptomatic, but clinically intestinal amoebiasis or extra intestinal amoebiasis may result. genital amoebiasis is very rare manifestation of extra intestial amoebiasis, but a case of amoebic scrotal abscess, seen in surabaya. the invasive form of entamoeba histolytica trophozoite was seen in giemsa stained aspirate of the abscess. in case of an abscess bacteria are primarly considered, but the case presented here shows that amoeba can be the cause, although ... | 2015 | 26695210 |
| the entamoeba histolytica, arp2/3 complex is recruited to phagocytic cups through an atypical kinase ehak1. | the parasite entamoeba histolytica is the etiological agent of amoebiasis and phagocytosis plays a key role in virulence of this organism. signaling pathways involved in activation of cytoskeletal dynamics required for phagocytosis remain to be elucidated. phagocytosis is initiated with sequential recruitment of ehc2pk, ehcabp1, ehcabp3 and an atypical kinase ehak1 after particle attachment. here we show that eharpc1, an essential subunit of the actin branching complex arp 2/3 is recruited to th ... | 2015 | 26646565 |
| ent-kaurene glycosides from ageratina cylindrica. | the aqueous extract of the leaves of ageratina cylindrica afforded six new ent-kaurenoic acid glycosides together with the known diterpenoid paniculoside v, the flavonoid astragalin, chlorogenic acid, and l-chiro-inositol. the structures were elucidated mainly by nmr and ms methods, and the absolute configuration was established by vibrational circular dichroism spectroscopy. the new compounds showed moderate antiprotozoal activity against entamoeba histolytica and giardia lamblia trophozoites. | 2015 | 26517282 |
| determining intestinal parasitic infections (ipis) in inmates from kajang prison, selangor, malaysia for improved prison management. | the prison management in malaysia is proactively seeking to improve the health status of the prison inmates. intestinal parasitic infections (ipis) are widely distributed throughout the world and are still gaining great concern due to their significant morbidity and mortality among infected humans. in malaysia, there is a paucity of information on ipis among prison inmates. in order to further enhance the current health strategies employed, the present study aims to establish firm data on the pr ... | 2015 | 26511347 |
| calpain-like: a ca(2+) dependent cystein protease in entamoeba histolytica cell death. | entamoeba histolytica programmed cell death (pcd) induced by g418 is characterized by the release of important amounts of intracellular calcium from reservoirs. nevertheless, no typical caspases have been detected in the parasite, the pcd phenotype is inhibited by the cysteine protease inhibitor e-64. these results strongly suggest that ca(2+)-dependent proteases could be involved in pcd. in this study, we evaluate the expression and activity of a specific dependent ca(2+) protease, the calpain- ... | 2015 | 26496790 |
| new drug target in protozoan parasites: the role of thioredoxin reductase. | amebiasis causes approximately 70,000 deaths annually and is the third cause of death due to parasites worldwide. it is treated primarily with metronidazole, which has adverse side effects, is mutagenic and carcinogenic, and emergence of resistance is an increasing concern. unfortunately, better therapeutic alternatives are lacking. re-purposing of older fda approved drugs is advantageous to drug discovery since safety and pharmacokinetic effects in humans are already known. in high throughput s ... | 2015 | 26483758 |
| [mean platelet volume in children with acute gastroenteritis caused by entamoeba histolytica]. | mean platelet volume (mpv) is a marker of platelet activation, which is a determinant of inflammation. the first aim of the present study was to investigate the mpv levels in children with amebiasis and compare the mpv levels with healthy controls. the second aim of this study was to evaluate the relationship between mpv and other acute phase reactants. | 2015 | 26470926 |
| [evaluation of two methods (nativ-lugol preparation and enzyme-linked immunosorbent assay) for detection of entamoeba histolytica in stool samples]. | this study aims to compare the performance of native-lugol examination and eia antigen detection test using stool samples obtained from patients diagnosed as clinical gastroenteritis and submitted to the parasitology laboratory in uludağ university between january 2010 and february 2011. | 2015 | 26470922 |
| molecular epidemiology of entamoeba: first description of entamoeba moshkovskii in a rural area from central colombia. | entamoeba histolytica, e. dispar and e. moshkovskii are the most frequent species described in human infection where e. histolytica is the only true pathogen. the epidemiology of this infection is complex due to the absence of a routine exam that allows a correct discrimination of the entamoeba species complex. therefore, molecular methods appear as the unique epidemiological tool to accomplish the species discrimination. herein, we conducted a cross-sectional study to determine the frequency of ... | 2015 | 26465744 |
| amoeboid movement in protozoan pathogens. | entamoeba histolytica, the causative agent of amoebiasis, is a protozoan parasite characterised by its amoeboid motility, which is essential to its survival and invasion of the human host. elucidating the molecular mechanisms leading to invasion of human tissues by e. histolytica requires a quantitative understanding of how its cytoskeleton deforms and tailors its mode of migration to the local microenvironment. here we review the wide range of methods available to extract biophysical informatio ... | 2015 | 26459974 |
| identification of natural inhibitors of entamoeba histolytica cysteine synthase from microbial secondary metabolites. | amebiasis is a common worldwide diarrheal disease, caused by the protozoan parasite, entamoeba histolytica. metronidazole has been a drug of choice against amebiasis for decades despite its known side effects and low efficacy against asymptomatic cyst carriers. e. histolytica is also capable of surviving sub-therapeutic levels of metronidazole in vitro. novel drugs with different mode of action are therefore urgently needed. the sulfur assimilatory de novo l-cysteine biosynthetic pathway is esse ... | 2015 | 26441896 |
| characterization of the protein tyrosine phosphatase prl from entamoeba histolytica. | protein tyrosine phosphatase of regenerating liver (prl) is a group of phosphatases that has not been broadly studied in protozoan parasites. in humans, prls are involved in metastatic cancer, the promotion of cell migration and invasion. ptps have been increasingly recognized as important effectors of host-pathogen interactions. we characterized the only putative protein tyrosine phosphatase prl (ptp ehprl) in the eukaryotic human intestinal parasite entamoeba histolytica. here, we reported tha ... | 2015 | 26431820 |
| intestinal amoebiasis in a patient with acute graft-versus-host disease after allogeneic bone marrow transplantation successfully treated by metronidazole. | amoebiasis has rarely been reported in patients undergoing hematopoietic stem cell transplantation, although it is a world-wide infection and extremely common. we present a case of intestinal amoebiasis unexpectedly revealed by colonoscopy after allogeneic bone marrow transplantation from a human leukocyte antigen-mismatched unrelated donor for acute myeloid leukemia arising from chronic myelomonocytic leukemia and successfully treated by metronidazole. | 2015 | 26426525 |
| entamoeba histolytica dmc1 catalyzes homologous dna pairing and strand exchange that is stimulated by calcium and hop2-mnd1. | meiosis depends on homologous recombination (hr) in most sexually reproducing organisms. efficient meiotic hr requires the activity of the meiosis-specific recombinase, dmc1. previous work shows dmc1 is expressed in entamoeba histolytica, a eukaryotic parasite responsible for amoebiasis throughout the world, suggesting this organism undergoes meiosis. here, we demonstrate dmc1 protein is expressed in e. histolytica. we show that purified ehdmc1 forms presynaptic filaments and catalyzes atp-depen ... | 2015 | 26422142 |
| the amoebicidal effect of ergosterol peroxide isolated from pleurotus ostreatus. | dysentery is an inflammation of the intestine caused by the protozoan parasite entamoeba histolytica and is a recurrent health problem affecting millions of people worldwide. because of the magnitude of this disease, finding novel strategies for treatment that does not affect human cells is necessary. ergosterol peroxide is a sterol particularly known as a major cytotoxic agent with a wide spectrum of biological activities produced by edible and medicinal mushrooms. the aim of this report is to ... | 2015 | 26392373 |
| is procalcitonin increased in cases of invasive amoebiasis? a retrospective, observational study. | procalcitonin (pct) levels are commonly used for diagnostic guidance in routine bacterial infections. by contrast, little data are currently available regarding pct in parasitic diseases, and its role in cases of invasive amoebiasis has not yet been described. for this purpose, 35 adult patients with a proven diagnosis of invasive or digestive amoebiasis were included in a 4-year study period. serum pct was retrospectively assessed. results were analysed with regard to the usual inflammatory bio ... | 2015 | 26388549 |
| evidence that the entamoeba histolytica mitochondrial carrier family links mitosomal and cytosolic pathways through exchange of 3'-phosphoadenosine 5'-phosphosulfate and atp. | entamoeba histolytica, a microaerophilic protozoan parasite, possesses mitosomes. mitosomes are mitochondrion-related organelles that have largely lost typical mitochondrial functions, such as those involved in the tricarboxylic acid cycle and oxidative phosphorylation. the biological roles of entamoeba mitosomes have been a long-standing enigma. we previously demonstrated that sulfate activation, which is not generally compartmentalized to mitochondria, is a major function of e. histolytica mit ... | 2015 | 26385892 |
| prevalence of entamoeba histolytica/entamoeba dispar in the city of campina grande, in northeastern brazil. | there is a clear need to perform epidemiological studies to find the true prevalence of entamoeba histolytica around the world. the evaluation of this prevalence has been hindered by the existence of two different species which are morphologically identical, but genetically different, namely e. histolytica, which causes amebiasis, and e. dispar, which is non-pathogenic. in brazil, the e. dispar has been detected in communities in the southeastern (se) and northeastern (ne) regions with poor sani ... | 2015 | 25229229 |
| bioconjugated fluorescent silica nanoparticles for the rapid detection of entamoeba histolytica. | rapid detection of entamoeba histolytica based on fluorescent silica nanoparticle (fsnp) indirect immunofluorescence microscopy was evaluated. silica nanoparticles were synthesized using stöber's method, with their surface activated to covalently bind to, and immobilize, protein a. for biolabeling, fsnp was added to conjugated e. histolytica trophozoites with monoclonal anti-e. histolytica igg1 for microscopic observation of fluorescence. fluorescent silica nanoparticle sensitivity was determine ... | 2015 | 25697865 |
| entamoeba infections in different populations of dogs in an endemic area of lahore, pakistan. | entamoeba histolytica, a protozoan parasite that affects humans and other primates all over the world. it is a common waterborne pathogen in endemic areas that have fecal oral transmission cycle. the aim of the present study was to examine the prevalence of e. histolytica and other entamoeba species cysts in three different dog populations. fecal samples from 600 dogs were collected and processed to detect entamoeba cysts using the triple fecal test (light microscopy) and fecal antigens of e. hi ... | 2015 | 25557213 |
| [investigation on entamoeba histolytica infection in diarrhea patients from general hospitals in shanghai city]. | to understand the status of entamoeba histolytica infection in diarrhea patients in general hospitals, so as to provide the evidences for the prevention and control of the disease. | 2015 | 27097477 |
| heavy burden of intestinal parasite infections in kalena rongo village, a rural area in south west sumba, eastern part of indonesia: a cross sectional study. | intestinal parasitic infections (ipis) are one of the major public health problems, especially in the rural area of developing countries with low socio-economic status and poor sanitation. the study was aimed to determine the prevalence of ipis among the inhabitants of a rural area in south west sumba, eastern part of indonesia. | 2015 | 26702820 |
| coinfection with malaria and intestinal parasites, and its association with anaemia in children in cameroon. | the purpose of this study was to determine the prevalence of coinfection with malaria and intestinal parasites, as well as to determine its association with anaemia in children aged 10 years and below in muyuka, cameroon. | 2015 | 26445484 |
| infection by intestinal parasites, stunting and anemia in school-aged children from southern angola. | intestinal parasites are responsible for morbidity in children worldwide, especially in low income countries. in the present study we determine the prevalence of intestinal parasites and explore its association with anemia and stunting in school-aged children. | 2015 | 26371758 |
| pervasiveness of intestinal protozoan and worm incursion in idp's (north waziristan agency, kpk-pakistan) children of 6-16 years. | to evaluate the prevalence of intestinal protozoan and worm infestation in children of families having migrated due to military operation. | 2015 | 26338737 |
| [intestinal parasitism in terena indigenous people of the province of mato grosso do sul, brazil]. | considering that intestinal protozoans and helminths infect more than half of the world population, with high prevalence in the poorest regions, the objective of this study was to conduct parasitological research among indigenous terena people established in the state of matogrosso do sul. an inquiry was performed to find the incidence of parasitism in these communities. | 2015 | 26120856 |
| predicting frequency distribution and influence of sociodemographic and behavioral risk factors of schistosoma mansoni infection and analysis of co-infection with intestinal parasites. | geospatial analysis was used to study the epidemiology of schistosoma mansoni, intestinal parasites and co-infections in an area (ilha das flores) in sergipe, brazil. we collected individually georeferenced sociodemographic, behavioral and parasitological data from 500 subjects, analyzed them by conventional statistics, and produced risk maps by kernel estimation. the prevalence rates found were: s. mansoni (24.0%), trichuris trichiura (54.8%), ascaris lumbricoides (49.2%), hookworm (17.6%) and ... | 2015 | 26054512 |
| dramatic decrease in prevalence of soil-transmitted helminths and new insights into intestinal protozoa in children living in the chaco region, bolivia. | we assessed the prevalence of intestinal parasites among 268 2-12-year-old children living in rural areas, small villages, and semi-urban areas of the chaco region, south-eastern bolivia. the overall parasitism was 69%. only protozoa, helminths, or co-infections were observed in 89.2%, 5.9%, or 4.9% of the positive children, respectively. a significant progressive increase in overall parasite prevalence was found when passing from rural areas to small villages and semi-urban areas. the most comm ... | 2015 | 25711609 |
| cellular cytokine and chemokine responses to parasite antigens and fungus and mite allergens in children co-infected with helminthes and protozoa parasites. | in sub-saharan africa poly-parasite infections are frequently observed in children, and with poly-parasitism modulating immune mechanisms, mediated by cytokines and chemokines, are required to prevent overwhelming inflammation and host tissue damage. we analyzed in children co-infected with helminthes and protozoan parasites their cellular production of regulatory and pro-inflammatory cytokines and chemokines in response to parasite antigens and allergens. | 2015 | 25698903 |
| identification of human intestinal parasites affecting an asymptomatic peri-urban argentinian population using multi-parallel quantitative real-time polymerase chain reaction. | in resource-limited countries, stool microscopy is the diagnostic test of choice for intestinal parasites (soil-transmitted helminths and/or intestinal protozoa). however, sensitivity and specificity is low. improved diagnosis of intestinal parasites is especially important for accurate measurements of prevalence and intensity of infections in endemic areas. | 2015 | 26183074 |
| anaerobic respiration: in vitro efficacy of nitazoxanide against mitochondriate acanthamoeba castellanii of the t4 genotype. | acanthamoeba is an opportunistic protist pathogen that is responsible for serious human and animal infection. being one of the most frequently isolated protists from the environment, it is likely that it readily encounters microaerophilic environments. for respiration under anaerobic or low oxygen conditions in several amitochondriate protists, decarboxylation of pyruvate is catalyzed by pyruvate ferredoxin oxidoreductase instead of pyruvate dehydrogenase. in support, nitazoxanide, an inhibitor ... | 2015 | 26297676 |
| a comparative study of bacterial and parasitic intestinal infections in india. | infectious diarrhea causes a major health problem in developing countries with significant morbidity and mortality. very often, rehydration therapy alone does not suffice, mandating the use of antimicrobial agents. however, rapidly decreasing antimicrobial susceptibility is complicating the matters. | 2015 | 25954615 |
| molecular and biochemical characterization of entamoeba histolytica fructokinase. | entamoeba histolytica is the causative agent of amoebic dysentery and liver abscess. the medium for its axenic culture contains glucose as energy source, and we addressed the question whether e. histolytica can also use fructose instead. as the amoebic hexokinases do not phosphorylate fructose, a separate fructokinase is essential. the genome project revealed a single candidate gene encoding an e. histolytica homolog of bacterial fructokinases. this gene was cloned, and the recombinant enzyme ha ... | 2015 | 25700717 |
| multicenter evaluation of the biofire filmarray gastrointestinal panel for etiologic diagnosis of infectious gastroenteritis. | the appropriate treatment and control of infectious gastroenteritis depend on the ability to rapidly detect the wide range of etiologic agents associated with the disease. clinical laboratories currently utilize an array of different methodologies to test for bacterial, parasitic, and viral causes of gastroenteritis, a strategy that suffers from poor sensitivity, potentially long turnaround times, and complicated ordering practices and workflows. additionally, there are limited or no testing met ... | 2015 | 25588652 |
| serological investigation to identify risk factors for post-flood infectious diseases: a longitudinal survey among people displaced by typhoon morakot in taiwan. | after typhoon morakot struck taiwan in 2009, thousands of taiwanese citizens were displaced to shelters for several weeks. others were placed in urban communities where they had family members. this study aimed to investigate serological status in both groups and identify risk factors associated with seroconversion of infectious diseases. | 2015 | 25976763 |
| structure of uridine diphosphate n-acetylglucosamine pyrophosphorylase from entamoeba histolytica. | uridine diphosphate n-acetylglucosamine pyrophosphorylase (uap) catalyzes the final step in the synthesis of udp-glcnac, which is involved in cell-wall biogenesis in plants and fungi and in protein glycosylation. small-molecule inhibitors have been developed against uap from trypanosoma brucei that target an allosteric pocket to provide selectivity over the human enzyme. a 1.8 å resolution crystal structure was determined of uap from entamoeba histolytica, an anaerobic parasitic protozoan that c ... | 2015 | 25945709 |
| microbial aetiology of acute diarrhoea in children under five years of age in khartoum, sudan. | diarrhoea is one of leading causes of morbidity and mortality worldwide. recent estimations suggested the number of deaths is close to 2.5 million. this study examined the causative agents of diarrhoea in children under 5 years of age in suburban areas of khartoum, sudan. a total of 437 stool samples obtained from children with diarrhoea were examined by culture and pcr for bacteria, by microscopy and pcr for parasites and by immunoassay for detection of rotavirus a. of the 437 samples analysed, ... | 2015 | 25713206 |
| prevalence of anal human papillomavirus infection and risk factors among hiv-positive patients in tokyo, japan. | oncogenic human papillomavirus (hpv) infection, particularly multiple hpv types, is recognized as a necessary cause of anal cancer. however, a limited number of studies have reported the prevalence of anal hpv infection in asia. we determined the prevalence, genotypes, and risk factors for anal hpv infection in japanese hiv-positive men who have sex with men (msm), heterosexual men, and women. | 2015 | 26368294 |
| phenotypic and transcriptional profiling in entamoeba histolytica reveal costs to fitness and adaptive responses associated with metronidazole resistance. | antimicrobial chemotherapy is critical in the fight against infectious diseases caused by entamoeba histolytica. among the drugs available for the treatment of amebiasis, metronidazole (mtz) is considered the drug of choice. recently, in vitro studies have described mtz resistance and the potential mechanisms involved. costs to fitness and adaptive responses associated with resistance, however, have not been investigated. in this study we generated an hm-1 derived strain resistant to 12 μm mtz ( ... | 2015 | 25999919 |
| the nlrp3 inflammasome is a pathogen sensor for invasive entamoeba histolytica via activation of α5β1 integrin at the macrophage-amebae intercellular junction. | entamoeba histolytica (eh) is an extracellular protozoan parasite of humans that invades the colon to cause life-threatening intestinal and extra-intestinal amebiasis. colonized eh is asymptomatic, however, when trophozoites adhere to host cells there is a considerable inflammatory response that is critical in the pathogenesis of amebiasis. the host and/or parasite factors that trigger the inflammatory response to invading eh are not well understood. we recently identified that eh adherence to m ... | 2015 | 25955828 |
| discovery of ppi-type phosphoenolpyruvate carboxykinase genes in eukaryotes and bacteria. | phosphoenolpyruvate carboxykinase (pepck) is one of the pivotal enzymes that regulates the carbon flow of the central metabolism by fixing co2 to phosphoenolpyruvate (pep) to produce oxaloacetate or vice versa. whereas atp- and gtp-type pepcks have been well studied, and their protein identities are established, inorganic pyrophosphate (ppi)-type pepck (ppi-pepck) is poorly characterized. despite extensive enzymological studies, its protein identity and encoding gene remain unknown. in this stud ... | 2015 | 26269598 |
| molecular epidemiology of amoebiasis: a cross-sectional study among north east indian population. | epidemiological studies carried out using culture or microscopy in most of the amoebiasis endemic developing countries, yielded confusing results since none of these could differentiate the pathogenic entamoeba histolytica from the non-pathogenic entamoeba dispar and entamoeba moshkovskii. the northeastern part of india is a hot spot of infection since the climatic conditions are most conducive for the infection and so far no systemic study has been carried out in this region. | 2015 | 26633890 |
| evaluation of factors affecting real-time pcr performance for diagnosis of entamoeba histolytica and entamoeba dispar in clinical stool samples. | although pcr offers the potential for sensitive detection of parasites there are several pitfalls for optimal performance, especially when dna is extracted from a complex sample material such as stool. with the aid of a sensitive inhibitor control in a duplex real-time pcr (qpcr) for identification of entamoeba histolytica and entamoeba dispar we have evaluated factors that influenced the performance of the qpcr and have suggested a rationale to be used in the analysis of clinical samples. pre-p ... | 2015 | 26296348 |
| cell-matrix interactions of entamoeba histolytica and e. dispar. a comparative study by electron-, atomic force- and confocal microscopy. | invasion of tissues by entamoeba histolytica is a multistep process that initiates with the adhesion of the parasite to target tissues. the recognition of the non-invasive entamoeba dispar as a distinct, but closely related protozoan species raised the question as to whether the lack of its pathogenic potential could be related to a weaker adhesion due to limited cytoskeleton restructuring capacity. we here compared the adhesion process of both amebas to fibronectin through scanning, transmissio ... | 2015 | 26231438 |
| intestinal amebae. | among the entamoeba species that infect humans, entamoeba histolytica causes diseases, entamoeba dispar is a harmless commensal, entamoeba moshkovskii seems to be a pathogen, and the pathogenicity of entamoeba bangladeshi remains to be investigated. species-specific detection needed for treatment decisions and for understanding the epidemiology and pathogenicity of these amebae. antigen-based detection methods are needed for e dispar, e moshkovskii, and e bangladeshi; and molecular diagnostic te ... | 2015 | 26004649 |
| maldi-tof mass spectrometry for the detection and differentiation of entamoeba histolytica and entamoeba dispar. | detection of entamoeba histolytica and its differentiation from entamoeba dispar is an important goal of the clinical parasitology laboratory. the aim of this study was the identification and differentiation of e. histolytica and e. dispar by maldi-tof ms, in order to evaluate the application of this technique in routine diagnostic practice. maldi-tof ms was applied to 3 amebic reference strains and to 14 strains isolated from feces that had been differentiated by molecular methods in our labora ... | 2015 | 25874612 |
| [environmental conditions and prevalence of parasitic infection in xukuru-kariri indigenous people, caldas, brazil]. | to describe the environmental conditions and the parasitic infection status of xukuru-kariri individuals living in the municipality of caldas, state of minas gerais, brazil. | 2015 | 26506320 |
| intestinal parasites in patients with chronic abdominal pain. | information about intestinal parasites in sohag (upper egypt) in patients with chronic abdominal pain is scarce. this study determined the intestinal parasites symptoms in 130 patients with chronic abdominal pain and cross-matched 20 healthy persons. parasitic infection was confirmed by stool analysis.the most commonest clinical data with stool analysis was as following: 1-entamoeba histolytica associated with nausea 20 (3 7.74%) followed by anorexia 19 (35.85%), 2-entamoeba coli associated with ... | 2015 | 26485858 |
| evolution of the prevalence of intestinal parasitosis in the region of tunis from 1996 at 2012. | the prevalence of intestinal parasitosis is very different according to countries. therefore, it is always interesting to update the data in tunisia to better direct control measures. | 2015 | 27126425 |
| [intestinal parasitosis among non-permanent resident students in tunisia: a review of 23 years of monitoring in the department of parasitology-mycology at the rabta hospital of tunisia]. | in order to fight digestive parasitism in tunisia, a national program of surveillance of non-permanent resident students in tunisia has been found to detect these parasitosis in this target population. | 2015 | 26757499 |
| prevalence of intestinal protozoa among saudi patients with chronic renal failure: a case-control study. | it has been hypothesized that chronic renal failure (crf) predisposes patients to infection with intestinal protozoa. we tested this hypothesis with a matched case-control study to determine the prevalence of these protozoa and their diarrhea associated symptoms among 50 patients with crf (cases) from taif, western saudi arabia. fifty diarrheal patients without crf were recruited in the study as controls. participants were interviewed by a structured questionnaire and stool samples were collecte ... | 2015 | 26491455 |
| prevalence of intestinal opportunistic parasites infections in the university hospital of bobo-dioulasso, burkina faso. | gastrointestinal parasites infections are widespread in africa and their prevalence infections vary from country to country. this study aimed at assessing the prevalence of opportunistic intestinal parasites infection and other gastrointestinal parasites infection among patients attending the laboratory of parasitology and mycology of the university hospital souro sanou of bobo-dioulasso. | 2015 | 26217488 |
| prevalence of intestinal protozoan infections among individuals living with hiv/aids at felegehiwot referral hospital, bahir dar, ethiopia. | hiv infection continues to pose a serious challenge to global health by predisposing patients to opportunistic infections. a cross-sectional study was conducted from december 2012 to february 2013 to assess the enteric protozoan infection status among individuals living with hiv/aids in felegehiwot referral hospital, bahir dar, northwest ethiopia. | 2015 | 25917962 |
| improved orthologous databases to ease protozoan targets inference. | homology inference helps on identifying similarities, as well as differences among organisms, which provides a better insight on how closely related one might be to another. in addition, comparative genomics pipelines are widely adopted tools designed using different bioinformatics applications and algorithms. in this article, we propose a methodology to build improved orthologous databases with the potential to aid on protozoan target identification, one of the many tasks which benefit from com ... | 2015 | 26416523 |
| antiparasitic activity of silver and copper oxide nanoparticles against entamoeba histolytica and cryptosporidium parvum cysts. | nanoparticles (nps) have received more attention as antiparasitic agents. in the present study, silver and copper nanoparticles were synthesized and characterized using scanning electron microscopy (sem), transmission electron microscope (tem) and x-ray fluorescence (xrf). the antiparasitic activity of ag and cuo nanoparticles were tested against two of the most environmentally spread parasites in egypt (entamoeba histolytica and cryptosporidium parvum). the average sizes of synthesized ag nps a ... | 2015 | 26939237 |
| intestinal parasites in diabetic patients in sohag university hospitals, egypt. | intestinal parasites usually create benign diseases, though they may induce complications with high morbidity and mortality to the immunocompromised, including diabetic patients. the study detected the prevalence of intestinal parasitic infections in diabetic patients, comparing to non-diabetic controls and other parameters. a total of 100 fecal samples were collected from diabetic patients at the outpatient clinic of sohag university hospitals and another 100 from cross matched controls. the sa ... | 2015 | 26485865 |
| prevalence of cryptosporidium parvum/hominis, entamoeba histolytica and giardia lamblia among young children with and without diarrhea in dar es salaam, tanzania. | although enteroparasites are common causes of diarrheal illness, few studies have been performed among children in tanzania. this study aimed to investigate the prevalence of cryptosporidium parvum/hominis, entamoeba histolytica and giardia lamblia among young children in dar es salaam, tanzania, and identify risk factors for infection. | 2015 | 26452235 |
| burden of major diarrheagenic protozoan parasitic co-infection among amoebic dysentery cases from north east india: a case report. | intestinal diarrheagenic polyparasitic infections are among the major public health concerns in developing countries. here we examined stool specimens by microscopy, dna dot blot and polymerase chain reaction (pcr) to evaluate the co-infection of four principal protozoans among amoebic dysentery cases from northeast indian population. the multiplex pcr confirmed entamoeba histolytica (8.1%), entamoeba dispar (4.8%) and mixed infection of both the parasites (3.4%) in 68 of 356 stool specimens tha ... | 2015 | 26099490 |
| [evaluation of the new immunocard stat!® cge test for the diagnosis of amebiasis]. | for many years, microscopic examination of stool samples has been considered to be the "gold standard" for diagnosis of intestinal parasites although the polymerase chain reaction (pcr) analysis is increasingly utilized due to its high accuracy. recently, pcr has been approved by the world health organization as the current method of choice for the diagnosis of entamoeba histolytica infection. in this study we evaluated a novel immunochromatographic antigen detection rapid test, immunocardstat c ... | 2015 | 26018388 |
| in vitro studies of chromone-tetrazoles against pathogenic protozoa, bacteria, and fungi. | in vitro studies to fourteen previously synthesized chromone-tetrazoles and four novel fluorine-containing analogs were conducted against pathogenic protozoan (entamoeba histolytica), pathogenic bacteria (pseudomonas aeruginosa, and staphylococcus aureus), and human fungal pathogens (sporothrix schenckii, candida albicans, and candida tropicalis), which have become in a serious health problem, mainly in tropical countries. | 2015 | 26184131 |
| diversity and prevalence of gastrointestinal parasites in seven non-human primates of the taï national park, côte d'ivoire. | parasites and infectious diseases are well-known threats to primate populations. the main objective of this study was to provide baseline data on fecal parasites in the cercopithecid monkeys inhabiting côte d'ivoire's taï national park. seven of eight cercopithecid species present in the park were sampled: cercopithecus diana, cercopithecus campbelli, cercopithecus petaurista, procolobus badius, procolobus verus, colobus polykomos, and cercocebus atys. we collected 3142 monkey stool samples betw ... | 2015 | 25619957 |
| amoebiasis masquerading as inflammatory bowel disease. | a 60-year-old japanese man presented with bloody diarrhoea. he stated that he had been diagnosed with ulcerative colitis (uc) 3 years prior, but discontinued follow-up care as treatment was ineffective. one year later, he came to our hospital with anorexia and weight loss. the abdomen was soft and flat without tenderness. laboratory tests were unremarkable; faecal culture and clostridium difficile toxin were negative. findings and biopsy from a subsequent colonoscopy reconfirmed his diagnosis of ... | 2015 | 26607188 |
| metronidazole-triazole conjugates: activity against clostridium difficile and parasites. | metronidazole has been used clinically for over 50 years as an antiparasitic and broad-spectrum antibacterial agent effective against anaerobic bacteria. however resistance to metronidazole in parasites and bacteria has been reported, and improved second-generation metronidazole analogues are needed. the copper catalysed huigsen azide-alkyne 1,3-dipolar cycloaddition offers a way to efficiently assemble new libraries of metronidazole analogues. several new metronidazole-triazole conjugates (mtz- ... | 2015 | 26117821 |
| dbdiasnp: an open-source knowledgebase of genetic polymorphisms and resistance genes related to diarrheal pathogens. | diarrhea is a highly common infection among children, responsible for significant morbidity and mortality rate worldwide. after pneumonia, diarrhea remains the second leading cause of neonatal deaths. numerous viral, bacterial, and parasitic enteric pathogens are associated with diarrhea. with increasing antibiotic resistance among enteric pathogens, there is an urgent need for global surveillance of the mutations and resistance genes primarily responsible for resistance to antibiotic treatment. ... | 2015 | 25978092 |
| [the application and epidemiological research of xtag gpp multiplex pcr in the diagnosis of infectious diarrhea]. | to investigate the application value of xtag (®) gastrointestinal pathogen panel (xtag9(®) gpp) multiplex pcr in the early diagnosis of infectious diarrhea, and understand the epidemiology of intestinal diarrhea pathogens. | 2015 | 25916780 |
| spectrum of enteropathogens detected by the filmarray gi panel in a multicentre study of community-acquired gastroenteritis. | the european, multicentre, quarterly point-prevalence study of community-acquired diarrhoea (eucodi) analysed stool samples received at ten participating clinical microbiology laboratories (austria, finland, france, germany, greece, ireland, italy, portugal, romania, and the uk) in 2014. on four specified days, each local laboratory submitted samples from ≤20 consecutive patients to the austrian study centre for further testing with the filmarray gi panel (biofire diagnostics, salt lake city, ut ... | 2015 | 25908431 |